CA2488842A1 - Carbocyclic nucleoside analogs as rna-antivirals - Google Patents

Abstract

The present invention provides carbocyclic nucleoside compounds and certain derivatives thereof which are inhibitors of RNA-dependent RNA viral polymerase. These compounds are inhibitors of RNA-dependent RNA viral replication and are useful for the treatment of RNA-dependent RNA viral infection. They are particularly useful as inhibitors of hepatitis C virus (HCV) NS5B polymerase, as inhibitors of HCV replication, and/or for the treatment of hepatitis C infection. The invention also describes pharmaceutical compositions containing such carbocyclic nucleoside compounds alone or in combination with other agents active against RNA-dependent RNA viral infection, in particular HCV infection. Also disclosed are methods of inhibiting RNA-dependent RNA polymerase, inhibiting RNA-dependent RNA viral replication, and/or treating RNA-dependent RNA viral infection with the carbocyclic nucleoside compounds of the present invention

Description

TITLE OF THE INVENTION
CARBOCYCLIC NUCLEOSIDE DERIVATIVES AS INHIBITORS OF RNA-DEPENDENT RNA VIRAL POLYMERASE
FIELD OF THE INVENTION
The present invention is concerned with carbocyclic nucleoside compounds and certain derivatives thereof, their synthesis, and their use as inhibitors of RNA-dependent RNA viral polymerase. The compounds of the present invention are inhibitors of RNA-dependent RNA viral replication and are useful for the treatment of RNA-dependent RNA viral infection. They are particularly useful as inhibitors of hepatitis C virus (HCV) NSSB polymerase, as inhibitors of HCV
replication, and for the treatment of hepatitis C infection.
BACKGROUND OF THE INVENTION
Hepatitis C virus (HCV) infection is a major health problem that leads to chronic liver disease, such as cirrhosis and hepatocellular carcinoma, in a substantial number of infected individuals, estimated to be 2-15% of the world's population. There are an estimated 4.5 million infected people in the United States alone, according to the U.S. Center for Disease Control. According to the World Health Organization, there are more than 200 million infected individuals worldwide, with at least 3 to 4 million people being infected each year. Once infected, about 20%
of people clear the virus, but the rest harbor HCV the rest of their lives.
Ten to twenty percent of chronically infected individuals eventually develop liver-destroying cirrhosis or cancer. The viral disease is transmitted parenterally by contaminated blood and blood products, contaminated needles, or sexually and vertically from infected mothers or carrier mothers to their off-spring. Current treatments for HCV
infection, which are restricted to immunotherapy with recombinant interferon-a or pegylated interferon-a, alone or in combination with the nucleoside analog ribavirin, are effective in only about 50°Io of the infected population (J.
Gillis, "Doctors Close in on Hepatitis C Suppression, Data Show," Washington Post, Thursday, April 18, 2002). Moreover, there is no established vaccine for HCV. Consequently, there is a continuing need for improved therapeutic agents that effectively combat chronic HCV
infection. The state of the art in the treatment of HCV infection has been reviewed, and reference is made to the following publications: B. Dymock, et al., "Novel approaches to the treatment of hepatitis C virus infection," Antiviral Chemistry &

Chemotherapy, 11: 79-96 (2000); H. Rosen, et al., "Hepatitis C virus: current understanding and prospects for future therapies," Molecular Medicine Today, 5: 393-399 (1999); D. Moradpour, et al., "Current and evolving therapies for hepatitis C,"
European J. Gastroenterol. He ap tol., 11: 1189-1202 (1999); R.
Bartenschlager, "Candidate Targets for Hepatitis C Virus-Specific Antiviral Therapy,"
Intervirolo~y, 40: 378-393 (1997); G.M. Lauer and B.D. Walker, "Hepatitis C Virus Infection,"
N.
Engl. J. Med., 345: 41-52 (2001); B.W. Dymock, "Emerging therapies for hepatitis C
virus infection," Emerging Drugs, 6: 13-42 (2001); and C. Crabb, "Hard-Won Advances Spark Excitement about Hepatitis C," Science: 506-507 (2001); the contents of all of which are incorporated by reference herein in their entirety.
Different approaches to HCV therapy have been taken, which include the inhibition of viral serine proteinase (NS3 protease), helicase, and RNA-dependent RNA polymerise (NSSB), and the development of a vaccine.
The HCV virion is an enveloped positive-strand RNA virus with a single oligoribonucleotide genomic sequence of about 9600 bases which encodes a polyprotein of about 3,010 amino acids. The protein products of the HCV gene consist of the structural proteins C, E1, and E2, and the non-structural proteins NS2, NS3, NS4A and NS4B, and NSSA and NSSB. The nonstructural (NS) proteins are believed to provide the catalytic machinery for viral replication. The NS3 protease releases NSSB, the RNA-dependent RNA polymerise from the polyprotein chain.
HCV NSSB polymerise is required for the synthesis of a double-stranded RNA
from a single-stranded viral RNA that serves as a template in the replication cycle of HCV.
NSSB polymerise is therefore considered to be an essential component in the HCV
replication complex [see K. Ishi, et al., "Expression of Hepatitis C Virus Protein: Characterization of Its RNA Polymerise Activity and RNA Binding,"
Hepatology, 29: 1227-1235 (1999) and V. Lohmann, et al., "Biochemical and Kinetic Analyses of NSSB RNA-Dependent RNA Polymerise of the Hepatitis C Virus,"
Virolo~y, 249: 108-118 (1998)]. Inhibition of HCV NSSB polymerise prevents formation of the double-stranded HCV RNA and therefore constitutes an attractive approach to the development of HCV-specific antiviral therapies.
It has now been found that carbocyclic nucleoside compounds of the present invention and certain derivatives thereof are potent inhibitors of RNA-dependent RNA viral replication and in particular HCV replication. The 5'-triphosphate derivatives of these carbocyclic nucleoside compounds are inhibitors of RNA-dependent RNA viral polymerise and in particular HCV NSSB polymerise.
_2_ The instant carbocyclic nucleoside compounds and derivatives thereof are useful to treat RNA-dependent RNA viral infection and in particular HCV infection.
It is therefore an object of the present invention to provide carbocyclic nucleoside compounds and certain derivatives thereof which are useful as inhibitors of RNA-dependent RNA viral polymerase and in particular as inhibitors of HCV
NSSB polymerase.
It is another object of the present invention to provide carbocyclic nucleoside compounds and certain derivatives thereof which are useful as inhibitors of the replication of an RNA-dependent RNA virus and in particular as inhibitors of the replication of hepatitis C virus.
It is another object of the present invention to provide carbocyclic nucleoside compounds and certain derivatives thereof which are useful in the treatment of RNA-dependent RNA viral infection and in particular in the treatment of HCV infection.
It is another object of the present invention to provide pharmaceutical compositions comprising the carbocyclic nucleoside compounds of the present invention in association with a pharmaceutically acceptable carrier.
It is another object of the present invention to provide pharmaceutical compositions comprising the carbocyclic nucleoside compounds and derivatives thereof of the present invention for use as inhibitors of RNA-dependent RNA
viral polymerase and in particular as inhibitors of HCV NSSB polymerase.
It is another object of the present invention to provide pharmaceutical compositions comprising the carbocyclic nucleoside compounds and derivatives thereof of the present invention for use as inhibitors of RNA-dependent RNA
viral replication and in particular as inhibitors of HCV replication.
It is another object of the present invention to provide pharmaceutical compositions comprising the carbocyclic nucleoside compounds and derivatives thereof of the present invention for use in the treatment of RNA-dependent RNA
viral infection and in particular in the treatment of HCV infection.
It is another object of the present invention to provide pharmaceutical compositions comprising the carbocyclic nucleoside compounds and derivatives thereof of the present invention in combination with other agents active against an RNA-dependent RNA virus and in particular against HCV.

It is another object of the present invention to provide methods for the inhibition of RNA-dependent RNA viral polymerase and in particular for the inhibition of HCV NSSB polymerase.
It is another object of the present invention to provide methods for the inhibition of RNA-dependent RNA viral replication and in particular for the inhibition of HCV replication.
It is another object of the present invention to provide methods for the treatment of RNA-dependent RNA viral infection and in particular for the treatment of HCV infection.
It is another object of the present invention to provide methods for the treatment of RNA-dependent RNA viral infection in combination with other agents active against RNA-dependent RNA virus and in particular for the treatment of HCV
infection in combination with other agents active against HCV.
It is another object of the present invention to provide carbocyclic nucleoside compounds and certain derivatives thereof and their pharmaceutical compositions for use as a medicament for the inhibition of RNA-dependent RNA
viral replication and/or the treatment of RNA-dependent RNA viral infection and in particular for the inhibition of HCV replication and/or the treatment of HCV
infection.
It is another object of the present invention to provide for the use of the carbocyclic nucleoside compounds and certain derivatives thereof of the present invention and their pharmaceutical compositions for the manufacture of a medicament for the inhibition of RNA-dependent RNA viral replication and/or the treatment of RNA-dependent RNA viral infection and in particular for the inhibition of HCV
replication and/or the treatment of HCV infection.
These and other objects will become readily apparent from the detailed description which follows.
SUMMARY OF THE INVENTION
The present invention relates to compounds of structural formula I of the indicated stereochemical configuration:

R~ X Rs or a pharmaceutically acceptable salt thereof; wherein B is wN Y wN
or R8--<~
W ~ N R11 ;
X is CH2, CHF, CF2, or C=CHI;
YisNorC-R9;
WisOorS;
R1 is C~_q. alkenyl, C2_4 alkynyl, or C1_4 alkyl, wherein alkyl is unsubstituted or substituted with hydroxy, amino, C1_q. allcoxy, C1_q. allcylthio, or one to three fluorine atoms;
R~ is hydrogen, fluorine, amino, hydroxy, mercapto, C1_q. alkoxy, C1_g alkylcarbonyloxy, or C1_4 alkyl;
R3 and R4 are each independently selected from the group consisting of hydrogen, cyano, azido, halogen, hydroxy, mercapto, amino, C1_4 alkoxy, C1_g alkylcarbonyloxy, C~_4 alkenyl, C2_q. alkynyl, and C1_4 alkyl, wherein alkyl is unsubstituted or substituted with hydroxy, amino, C1_q. alkoxy, C1_q.
alkylthio, or one to three fluorine atoms;
R5 is hydrogen, C1_10 alkylcarbonyl, P309H4, P20(H3, or P(O)R13R14;
R6 and R~ are each independently hydrogen, methyl, hydroxymethyl, or fluoromethyl;
R~ is hydrogen, C1_4 alkyl, C~_q. alkynyl, halogen, cyano, carboxy, C1_4 allcyloxycarbonyl, azido, amino, C1_4 alkylamino, di(C1_q. alkyl)amino, hydroxy, C1_6 alkoxy, C1_6 alkylthio, C1_6 alkylsulfonyl, or (C1_4 alkyl)0_~
aminomethyl;
R9 is hydrogen, halogen, cyano, nitro, NHCONH2~ CONR1~R12, CSNR1~R12, COOR 1 ~, C(=NH)NH2, hydroxy, C 1 _3 alkoxy, amino, C 1 _4 alkylamino, di(C 1 _4 _5_ alkyl)amino, or C1_3 alkyl, wherein alkyl is unsubstituted or substituted with one to three groups independently selected from halogen, amino, hydroxy, carboxy, and C1_3 alkoxy;
R10 and R16 are each independently hydrogen, hydroxy, mercapto, halogen, C1-4 allcoxy, C1_q. alkylthio, C1_g alkylcarbonyloxy, C3_6 cycloalkylcarbonyloxy, C1_g alkyloxycarbonyloxy, C3_6 cycloalkyloxycarbonyloxy, -OCH2CH2SC(=O)C1_4 alkyl, -OCH20(C=O)C1_q. alkyl, -OCH(C1_q. alkyl)O(C=O)C1_q. alkyl, amino, C1_4 alkylamino, di(C1_q. alkyl)amino, C3_6 cycloalkylamino, di(C3_6 cycloalkyl)amino, or an amino acyl residue having structural formula R2o O R2o O
~N OR1~ °r ~N NR18R1s H ° H n ;
n is 0, 1, or 2;
R11 is hydrogen, hydroxy, halogen, C1_q. alkoxy, amino, C1_q. alkylamino, di(C1_q. alkyl)amino, C3_6 cycloalkylamino, or di(C3_6 cycloalkylamino);
each R1~ is independently hydrogen or C1_6 alkyl;
R1~, Rlg, and R19 are each independently hydrogen or C1_6 alkyl;
R13 and R14 are each independently hydroxy, -OCH~CHZSC(=O)C1_q. alkyl, -OCH~O(C=O)OC1_q. alkyl, -NHCHMeCO~Me, -OCH(Cl_q. alkyl)O(C=O)C1_q.
alkyl, O~S~C~"'~2~11CH3 ~\~~S~CH2~17CH3 IO~CH2)gCH3 Of OCO~C~"~2)14CH3 R15 is hydrogen, C1_6 alkyl, C2_6 alkenyl, C2_6 alkynyl, Cl_q. alkylamino, CF3, or halogen; and R~0 is hydrogen, C1_q. alkyl, or phenyl Cp_~ alkyl;
with the proviso that when B is R~ o R8~/ I ~ N
N N~R11;
X is CH2; Y is N; R10 is NH2; R2 and R3 are a,-OH; and R4, R5, R6, R~, Rg, and R11 are hydrogen, then R1 is not (3-methyl.
The compounds of formula I are useful as inhibitors of RNA-dependent RNA viral polymerise and in particular of HCV NSSB polymerise. They are also inhibitors of RNA-dependent RNA viral replication and in particular of HCV
replication and are useful for the treatment of RNA-dependent RNA viral infection and in particular for the treatment of HCV infection.
Also encompassed within the present invention are pharmaceutical compositions containing the compounds alone or in combination with other agents active against RNA-dependent RNA virus and in particular against HCV as well as methods for the inhibition of RNA-dependent RNA viral replication and for the treatment of RNA-dependent RNA viral infection.
DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to compounds of structural formula I of the indicated stereochemical configuration:

R~ X Rs R~ R1 or a pharmaceutically acceptable salt thereof; wherein n is 0, l, or 2;
B is ~N Y ~N
or R$-C~
N R11;
X is CH2, CHF, CF2, or C=CH2;
Y is N or C-R9;
WisOorS;
R1 is C2_q. alkenyl, C2_q. alkynyl, or C1_q. alkyl, wherein alkyl is unsubstituted or substituted with hydroxy, amino, C 1 _q. alkoxy, C 1 _q. alkylthio, or one to three fluorine atoms;
R2 is hydrogen, fluorine, amino, hydroxy, mercapto, C1_q. alkoxy, C1_g alkylcarbonyloxy, or C1_q. alkyl;
R3 and R4 are each independently selected from the group consisting of hydrogen, cyano, azido, halogen, hydroxy, mercapto, amino, C1_q. alkoxy, C1_g alkylcarbonyloxy, CZ_q. alkenyl, C2_q. alkynyl, and C1_q. alkyl, wherein alkyl is unsubstituted or substituted with hydroxy, amino, C1_q. alkoxy, C1_q.
alkylthio, or one to three fluorine atoms;
R5 is hydrogen, 01_10 alkylcarbonyl, P3O9Hq., P20(H3, or P(O)R13R14;
R6 and R~ are each independently hydrogen, methyl, hydroxymethyl, or fluoromethyl;
Rg is hydrogen, C1_q. alkyl, C2_q. alkynyl, halogen, cyano, carboxy, C1_4 alkyloxycarbonyl, azido, amino, C1_q. alkylamino, di(C1_q. alkyl)amino, hydroxy, C1_6 alkoxy, C1_6 allcylthio, C1_6 alkylsulfonyl, or (C1_q. alkyl)p_2 aminomethyl;
R9 is hydrogen, halogen, cyano, nitro, NHCONH2~ CONR12R12, CSNR12R12, COOR12, C(=NH)NH2, hydroxy, C1_3 alkoxy, amino, C1_q. alkylamino, di(C1_4 alkyl)amino, or C1_3 alkyl, wherein alkyl is unsubstituted or substituted with one to three groups independently selected from halogen, amino, hydroxy, carboxy, and C1_3 alkoxy;
R10 and R16 are each independently hydrogen, hydroxy, mercapto, halogen, C1_4 alkoxy, C1_q. alkylthio, C1_g alkylcarbonyloxy, C3_6 cycloalkylcarbonyloxy, C1_g alkyloxycarbonyloxy, C3_6 cycloalkyloxycarbonyloxy, -OCH2CH2SC(=O)C1_4 alkyl, -OCH20(C=O)C1_q. alkyl, -OCH(C1_q. alkyl)O(C=O)C1_q. alkyl, amino, C1_4 alkylamino, di(C1_q. alkyl)amino, C3_6 cycloalkylamino, di(C3_6 cycloallcyl)amino, or an amino aryl residue having structural formula _g_ R2o O R2o O
~N OR1~ °r ~N NR18R1s R11 is hydrogen, hydroxy, halogen, C1_q. alkoxy, amino, C1_q. alkylamino, di(C1_q. alkyl)amino, C3_6 cycloalkylamino, or di(C3_g cycloalkylamino);
each R12 is independently hydrogen or C1_6 alkyl;
R1~, Rlg, and R19 are each independently hydrogen or C1_6 alkyl;
R13 and R14 are each independently hydroxy, -OCHZCHZSC(=O)C1_q. alkyl, -OCH20(C=O)OC1_q. alkyl, -NHCHMeC02Me, -OCH(C1_4 alkyl)O(C=O)C1_4 alleyl, ~~O~S(CH2)11CH3 ~~O~S(CH2)I~CHs O(CH~)9CH3 °r OCO(CH2)14~H3 R15 is hydrogen, C1_6 alkyl, CZ_6 alkenyl, C~_6 alkynyl, C1_q. alkylamino, CF3, or halogen; and R20 is hydrogen, C1_q. alkyl, or phenyl CO_~ alkyl;
with the proviso that when B is R1o R8~/ ~ ~ N
N N~R11;
X is CH2; Y is N; R10 is NHS; R~ and R3 are a-OH; and R4, R5, R6, R~, R8, and R11 are hydrogen, then R1 is not (3-methyl.
The compounds of formula I are useful as inhibitors of RNA-dependent RNA viral polymerase. They are also inhibitors of RNA-dependent RNA viral replication and are useful for the treatment of RNA-dependent RNA
viral infection.
In one embodiment of the compounds of structural formula I, B is R1o R8~% w N
N N~R11 In a class of this embodiment are compounds of structural formula II:
R~ o N N
R50 R8~/
N N~R11 wherein R1 is C1_3 alkyl, wherein alkyl is unsubstituted or substituted with one to three fluorine atoms;
R2 is hydroxy, fluoro, C1_3 alkoxy, or C1_g alkylcarbonyloxy;
R3 is hydrogen, halogen, hydroxy, amino, C1_3 alkoxy, or C1_g allcylcarbonyloxy;
R5 is hydrogen, C1_g alkylcarbonyl, P3O9Hq., P20(H3, or P03H2;
R$ is hydrogen, amino, or C1_q. alkylamino; and R10 and R11 are each independently hydrogen, halogen, hydroxy, amino, C1_q. alkylamino, di(C1_q. alkyl)amino, or C3_6 cycloalkylamino;
with the proviso that when R10 is NH2, R2 and R3 are a-OH, and R5, Rg, and R11 are hydrogen, then R1 is not (3-methyl.
In a subclass of this class, R1 is methyl, fluoromethyl, difluoromethyl, or trifluoromethyl;
R2 is hydroxy, fluoro, or rnethoxy;
R3 is hydrogen, fluoro, hydroxy, amino, or methoxy;
R5 is hydrogen or P309Hq.;
Rg is hydrogen or amino; and R10 and Rl1 are each independently hydrogen, fluoro, hydroxy, or amino;

with the proviso that when R10 is NH2, R2 and R3 are a-OH, and R5, R$, and R11 are hydrogen, then R1 is not [3-methyl.
In another class of this embodiment are compounds of structural formula III:

N
R5~ Rg 5 (III) wherein R1 is C1_3 alkyl, wherein alkyl is unsubstituted or substituted with one to three fluorine atoms;
R2 is hydroxy, fluoro, C1_3 alkoxy, or C1_g alkylcarbonyloxy;
10 R3 is hydrogen, halogen, hydroxy, amino, C1_3 alkoxy, or C1_g alkylcarbonyloxy;
R5 is hydrogen, C1_g alkylcarbonyl, P309Hq., P2O(H3, or P03H2;
Rg is hydrogen, amino, or C1_q. alkylamino;
R9 is hydrogen, cyano, methyl, halogen, CONH2 or CSNH2; and R10 and R11 are each independently hydrogen, halogen, hydroxy, amino, C1_q. alkylamino, di(C1_q. allcyl)amino, or C3_6 cycloalkylamino.
In a subclass of this class, Rl is methyl, fluoromethyl, difluoromethyl, or trifluoromethyl;
R2 is hydroxy, fluoro, or methoxy;
R3 is hydrogen, fluoro, hydroxy, amino, or methoxy;
R5 is hydrogen or P309Hq.;
R~ is hydrogen or amino;
R9 is hydrogen, cyano, methyl, halogen, CONH2 or CSNH2; and R10 and R11 are each independently hydrogen, fluoro, hydroxy, or amino.
In a third class of this embodiment are compounds of structural formula IV:

R1o R8 \N w N
R5p N N~R11 (IV) wherein R1 is C1_3 alkyl, wherein alkyl is unsubstituted or substituted with one to three fluorine atoms;
RZ is hydroxy, fluoro, C1_3 alkoxy, or C1_g alkylcarbonyloxy;
R3 is hydrogen, halogen, hydroxy, amino, C1_3 alleoxy, or C1_g alkylcarbonyloxy;
R5 is hydrogen, C1_g alkylcarbonyl, P309Hq., P~06H3, or P03H~;
R$ is hydrogen, amino, or C1_q. alkylamino; and R1o and R11 are each independently hydrogen, halogen, hydroxy, amino, C1_q. alkylamino, di(C1_q. alkyl)amino, or C3_6 cycloalkylamino.
In a subclass of this class R1 is methyl, fluoromethyl, difluoromethyl, or trifluoromethyl;
R2 is hydroxy, fluoro, or methoxy;
R3 is hydrogen, fluoro, hydroxy, amino, or methoxy;
R5 is hydrogen or P309Hq.;
R~ is hydrogen or amino; and R1~ and R11 are each independently hydrogen, fluoro, hydroxy, or amino.
In a fourth class of this embodiment are compounds of structural formula V:

R9 R1o R$ ~ I ~~N
R50 N~N~R11 (V) wherein R1 is C1_3 alkyl, wherein alkyl is unsubstituted or substituted with one to three fluorine atoms;
R2 is hydroxy, fluoro, C1_3 alkoxy, or C1_g alkylcarbonyloxy;
R3 is hydrogen, halogen, hydroxy, amino, C1_3 alkoxy, or C1_g alkylcarbonyloxy;
R5 is hydrogen, C1_g alkylcarbonyl, P309H4, P20gH3, or P03H2;
Rg is hydrogen, amino, or C1_q. alkylamino;
R9 is hydrogen, cyano, methyl, halogen, CONH2 or CSNH2; and R1~ and R11 are each independently hydrogen, halogen, hydroxy, amino, C1_q. alkylamino, di(C1_q. alkyl)amino, or C3_~ cycloalkylamino.
In a subclass of this class, R1 is methyl, fluoromethyl, difluoromethyl, or trifluoromethyl;
R2 is hydroxy, fluoro, or methoxy;
R3 is hydrogen, fluoro, hydroxy, amino, or methoxy;
R5 is hydrogen or P3O9H4;
Rg is hydrogen or amino;
R9 is hydrogen, cyano, methyl, halogen, CONH2 or CSNH2;
R1~ and R11 are each independently hydrogen, fluoro, hydroxy, or amino.

Illustrative, but nonlimiting, examples of compounds of the present invention of structural formula I which are useful as inhibitors of RNA-dependent RNA viral polymerase are the following:

2-amino-7-[(1(3,2aOH,3a,4(3)-2,3-dihydroxy-4-hydroxymethyl-2-methyl-5-methylenecyclopentyl]-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one;

2-amino-7-[(1R,2S,3R,4R)-2,3-dihydroxy-4-hydroxymethyl-2-methyl-5-methylenecyclopentyl]-3,7-dihydro-4H-pyrrolo [2,3-d]pyrimidin-4-one;
( 1 aOH,2a,3 (3,5 (3)-5-(4-amino-7H-pyrrolo [2,3-d]pyrimidin-7-yl)-3-hydroxymethyl-1-methyl-4- methylenecyclopentane-1,2-diol;
(1S,2R,3R,5R)-5-(4-amino-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-3-hydroxymethyl-1-methyl-4- methylenecyclopentane-1,2-diol;
(1 (3,2aOH,3a,4[3)-2-amino-9-[2,3-dihydroxy-4-(hydroxymethyl)-2-methyl-5-methylenecyclopentyl]-1,9-dihydro-6H-purin-6-one;
2-amino-9-[(1R,2S,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)-2-methyl-5-methylenecyclopentyl]-1,9-dihydro-6H-purin-6-one;
(1S,2R,3R,5R)-5-(6-amino-9H purin-9-yl)-3-(hydroxymethyl)-1-methyl-4-methylenecyclopentane-1,2-diol;
(laOH,2a,3[i,5[3)-5-(6-amino-9H-purin-9-yl)-3-(hydroxymethyl)-1-methyl-4-methylenecyclopentane-1,2-diol;
(1RS,2R,3R,5R)-5-(4-amino-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-3-(hydroxymethyl)-1-methylcyclopentanediol-1,2-diol;
( 1 S,2R,3R,5R)-5-(4-amino-7H-pyrrolo [2,3-d]pyrimidin-7-yl)-3-(hydroxymethyl)-methylcyclopentanediol-1,2-diol;
(1RS,2R,3R,5R)-5-(6-amino-9H purin-9-yl)-3-(hydroxymethyl)-1-methylcyclopentanediol-1,2-diol;
( 1 S,2R,3R,5R)-5-(6-amino-9H-purin-9-yl)-3-(hydroxymethyl)-1-methylcyclopentanediol-1,2-diol;

2-amino-9-[(1R,2RS,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)-2-methylcyclopentyl]-1,9-dihydro-6H-purin-6-one;
2-amino-9-[( 1R,2S,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)-2-methylcyclopentyl]-1,9-dihydro-6H-purin-6-one;
2-amino-7-[( 1R,2RS,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)-2-methylcyclopentyl]-3,7-dihydro-4H pyrrolo[2,3-d]pyrimidin-4-one; and 2-amino-7-[(1R,2S,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)-2-methylcyclopentyl]-3,7-dihydro-4H-pyrrolo [2, 3-d] pyrimidin-4-one;
and the corresponding 5'-triphosphates;
or a pharmaceutically acceptable salt thereof.
In one embodiment of the present invention, the carbocyclic nucleoside compounds of the present invention are useful as inhibitors of positive-sense single-stranded RNA-dependent RNA viral polymerise, inhibitors of positive-sense single-stranded RNA-dependent RNA viral replication, and/or for the treatment of positive-sense single-stranded RNA-dependent RNA viral infection. In a class of this embodiment, the positive-sense single-stranded RNA-dependent RNA virus is a Flaviviridae virus or a Picorv~aviridae virus. In a subclass of this class, the Picowaviridae virus is a rhinovirus, a poliovirus, or a hepatitis A virus. In a second subclass of this class, the Flaviviridae virus is selected from the group consisting of hepatitis C virus, yellow fever virus, dengue virus, West Nile virus, Japanese encephalitis virus, Banzi virus, and bovine viral diarrhea virus (BVDV). In a subclass of this subclass, the Flaviviridae virus is hepatitis C virus.
Another aspect of the present invention is concerned with a method for inhibiting RNA-dependent RNA viral polymerise, a method for inhibiting RNA-dependent RNA viral replication, and/or a method for treating RNA-dependent RNA
viral infection in a mammal in need thereof comprising administering to the mammal a therapeutically effective amount of a compound of structural formula I.
In one embodiment of this aspect of the present invention, the RNA-dependent RNA viral polymerise is a positive-sense single-stranded RNA-dependent RNA viral polymerise. In a class of this embodiment, the positive-sense single-stranded RNA-dependent RNA viral polymerise is a Flaviviridae viral polymerise or a Picornaviridae viral polymerase. In a subclass of this class, the Piconiaviridae viral polymerise is rhinovirus polymerise, poliovirus polymerise, or hepatitis A
virus polymerise. In a second subclass of this class, the Flaviviridae viral polymerise is selected from the group consisting of hepatitis C virus polymerise, yellow fever virus polymerise, dengue virus polymerise, West Nile virus polymerise, Japanese encephalitis virus polymerise, Banzi virus polymerise, and bovine viral diarrhea virus (BVDV) polymerise. In a subclass of this subclass, the Flaviviridae viral polymerise is hepatitis C virus polymerise.
In a second embodiment of this aspect of the present invention, the RNA-dependent RNA viral replication is a positive-sense single-stranded RNA-dependent RNA viral replication. In a class of this embodiment, the positive-sense single-stranded RNA-dependent RNA viral replication is Flaviviridae viral replication or PiconZaviridae viral replication. In a subclass of this class, the Piconzaviridae viral replication is rhinovirus replication, poliovirus replication, or hepatitis A virus replication. In a second subclass of this class, the Flaviviridae viral replication is selected from the group consisting of hepatitis C virus replication, yellow fever virus replication, dengue virus replication, West Nile virus replication, Japanese encephalitis virus replication, Banzi virus replication, and bovine viral diarrhea virus replication. In a subclass of this subclass, the Flaviviridae viral replication is hepatitis C virus replication.
In a third embodiment of this aspect of the present invention, the RNA-dependent RNA viral infection is a positive-sense single-stranded RNA-dependent viral infection. In a class of this embodiment, the positive-sense single-stranded RNA-dependent RNA viral infection is Flaviviridae viral infection or Picornaviridae viral infection. In a subclass of this class, the Picoraaviridae viral infection is rhinovirus infection, poliovirus infection, or hepatitis A virus infection. In a second subclass of this class, the Flaviviridae viral infection is selected from the group consisting of hepatitis C virus infection, yellow fever virus infection, dengue virus infection, West Nile virus infection, Japanese encephalitis virus infection, Banzi virus infection, and bovine viral diarrhea virus infection. In a subclass of this subclass, the Flaviviridae viral infection is hepatitis C virus infection.
Throughout the instant application, the following terms have the indicated meanings:
The alkyl groups specified above are intended to include those alkyl groups of the designated length in either a straight or branched configuration.

Exemplary of such alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, tertiary butyl, pentyl, isopentyl, hexyl, isohexyl, and the like.
The term "alkenyl" shall mean straight or branched chain alkenes of two to six total carbon atoms, or any number within this range (e.g., ethenyl, propenyl, butenyl, pentenyl, etc.).
The term "alkynyl" shall mean straight or branched chain alkynes of two to six total carbon atoms, or any number within this range (e.g., ethynyl, propynyl, butynyl, pentynyl, etc.).
The term "cycloalkyl" shall mean cyclic rings of alkanes of three to eight total carbon atoms, or any number within this range (i.e., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, or cyclooctyl).
The term "cycloheteroalkyl" is intended to include non-aromatic heterocycles containing one or two heteroatoms selected from nitrogen, oxygen and sulfur. Examples of 4-6-membered cycloheteroalkyl include azetidinyl, pyrrolidinyl, piperidinyl, morpholinyl, thiamorpholinyl, imidazolidinyl, tetrahydrofuranyl, tetrahydropyranyl, tetrahydrothiophenyl, piperazinyl, and the like.
The term "alkoxy" refers to straight or branched chain alkoxides of the number of carbon atoms specified (e.g., C1_q. alkoxy), or any number within this range [i.e., methoxy (MeO-), ethoxy, isopropoxy, etc.].
The term "alkylthio" refers to straight or branched chain alkylsulfides of the number of carbon atoms specified (e.g., C1_q. alkylthio), or any number within this range [i.e., methylthio (MeS-), ethylthio, isopropylthio, etc.].
The term "alkylamino" refers to straight or branched alkylamines of the number of carbon atoms specified (e.g., C1_q. alkylamino), or any number within this range [i.e., methylamino, ethylamino, isopropylamino, t-butylamino, etc.].
The term "cycloalkylamino" refers to saturated aminohydrocarbons containing one ring of the number of carbon atoms specified (e.g., C3-6 cycloalkylamino), or any number within this range [i.e., cyclopropylamino, cyclobutylamino, cyclopentylamino, and cyclohexylamino].
The term "allcylsulfonyl" refers to straight or branched chain alkylsulfones of the number of carbon atoms specified (e.g., C1_6 alkylsulfonyl), or any number within this range [i.e., methylsulfonyl (MeS02-), ethylsulfonyl, isopropylsulfonyl, etc.].
The term "alkyloxycarbonyl" refers to straight or branched chain esters of a carboxylic acid derivative of the present invention of the number of carbon atoms specified (e.g., C1_q. alkyloxycarbonyl), or any number within this range [i.e., methyloxycarbonyl (MeOCO-), ethyloxycarbonyl, or butyloxycarbonyl].
The term "aryl" includes both phenyl, naphthyl, and pyridyl. The phenyl, naphthyl, or pyridyl group wherever it occurs in the compounds of the present invention is optionally substituted with one to three groups independently selected from C1_q. alkyl, halogen, cyano, nitro, trifluoromethyl, C1_q. alkoxy, and C1-alkylthio.
The term "halogen" is intended to include the halogen atoms fluorine, chlorine, bromine and iodine.
The term "substituted" shall be deemed to include multiple degrees of substitution by a named substituent. Where multiple substituent moieties are disclosed or claimed, the substituted compound can be independently substituted by one or more of the disclosed or claimed substituent moieties, singly or plurally.
The term "amino acyl residue" refers to an a-, (3-, or y-amino acyl group of structural formula R2o O R2o O
~N OR1~ °r ~N NRl8Ris H n H n wherein n is 0, 1, or 2 and R1~, R18, R19, and R20 are as defined hereinabove.
When R20 is not hydrogen, the amino acyl residue contains an asymmetric center and is intended to include the individual R- and S-enantioners as well as RS-racemic mixtures.
The term "5'-triphosphate" refers to a triphosphoric acid ester derivative of the 5'-hydroxyl group of a carbocyclic nucleoside compound of the present invention having the following general structural formula:
O O O
HO~ P~O~ P~O~ P~O
OH OH O R~ X B Rs wherein B and R1-R11 are as defined above. The compounds of the present invention axe also intended to include pharmaceutically acceptable salts of the triphosphate ester as well as pharmaceutically acceptable salts of 5'-monophosphate and 5'-diphosphate ester derivatives of the structural formulae A and B, respectively, n n HO~P~O
O R~ X B Rs HO O O OHO X B
R~ R6 R4 R1 Ra. R1 (A) The term "5'-(S-acyl-2-thioethyl)phosphate" or "SATE" refers to a mono- or di-ester derivative of a 5'-monophosphate carbocyclic nucleoside derivative of the present invention of structural formulae C and I~, respectively, as well as pharmaceutically acceptable salts of the mono-ester, O
C1 ~S~O~P~O
O OH ~ X B s R R

to O
n C1 _4~5~0~ PLO
B
O O R~ X Rs R R
Rs R2 O
C1 4 (D) The term "composition", as in "pharmaceutical composition," is intended to encompass a product comprising the active ingredients) and the inert ingredients) that make up the can-ier, as well as any product which results, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients, or from dissociation of one or more of the ingredients, or from other types of reactions or interactions of one or more of the ingredients. Accordingly, the pharmaceutical compositions of the present invention encompass any composition made by admixing a compound of the present invention and a pharmaceutically acceptable carrier.
The terms "administration of ' and "administering a" compound should be understood to mean providing a compound of the invention or a prodrug of a compound of the invention to the individual in need.
Another aspect of the present invention is concerned with a method of inhibiting HCV NSSB polymerase, inhibiting HCV replication, or treating HCV
infection with a compound of the present invention in combination with one or more agents useful for treating HCV infection. Such agents active against HCV
include, but are not limited to, ribavirin, levovirin, viramidine, thymosin alpha-l, interferon-~3, interferon-a, pegylated interferon-a (peginterferon-a), a combination of interferon-a and ribavirin, a combination of peginterferon-a and ribavirin, a combination of interferon-a and levovirin, and a combination of peginterferon-a and levovirin.
Interferon-a includes, but is not limited to, recombinant interferon-a2a (such as Roferon interferon available from Hoffmann-LaRoche, Nutley, NJ), pegylated interferon-a2a (PegasysTM), interferon-a2b (such as Intron-A interferon available from Schering Corp., Kenilworth, NJ), pegylated interferon-a2b (PegIntron~), a recombinant consensus interferon (such as interferon alphacon-1), and a purified interferon-a product. Amgen's recombinant consensus interferon has the brand name Infergen0. Levovirin is the L-enantiomer of ribavirin which has shown immunomodulatory activity similar to ribavirin. Viramidine represents an analog of ribavirin disclosed in WO 01160379 (assigned to ICN Pharmaceuticals). In accordance with this method of the present invention, the individual components of the combination can be administered separately at different times during the course of therapy or concurrently in divided or single combination forms. The instant invention is therefore to be understood as embracing all such regimes of simultaneous or alternating treatment, and the term "administering" is to be interpreted accordingly. It will be understood that the scope of combinations of the compounds of this invention with other agents useful for treating HCV infection includes in principle any combination with any pharmaceutical composition for treating HCV infection.
When a compound of the present invention or a pharmaceutically acceptable salt thereof is used in combination with a second therapeutic agent active against HCV, the dose of each compound may be either the same as or different from the dose when the compound is used alone.
For the treatment of HCV infection, the compounds of the present invention may also be administered in combination with an agent that is an inhibitor of HCV NS3 serine protease. HCV NS3 serine protease is an essential viral enzyme and has been described to be an excellent target for inhibition of HCV
replication.
Both substrate and non-substrate based inhibitors of HCV NS3 protease inhibitors are disclosed in WO 98/22496, WO 98/46630, WO 99/07733, WO 99/07734, WO
99/38888, WO 99/50230, WO 99/64442, WO 00/09543, WO 00/59929, and GB-2337262. HCV NS3 protease as a target for the development of inhibitors of HCV
replication and for the treatment of HCV infection is discussed in B.W.
Dymock, "Emerging therapies for hepatitis C virus infection," Emer.~ing Drugs, 6: 13-(2001).
Ribavirin, levovirin, and viramidine may exert their anti-HCV effects by modulating intracellular pools of guanine nucleotides via inhibition of the intracellular enzyme inosine monophosphate dehydrogenase (M'DH). IIvVIPDH is the rate-limiting enzyme on the biosynthetic route in de novo guanine nucleotide biosynthesis. Ribavirin is readily phosphorylated intracellularly and the monophosphate derivative is an inhibitor of )IVVIPDH. Thus, inhibition of IMPDH
represents another useful target for the discovery of inhibitors of HCV
replication.
Therefore, the compounds of the present invention may also be administered in combination with an inhibitor of )IVVIPDH, such as VX-497, which is disclosed in WO
97/41211 and WO 01/00622 (assigned to Vertex); another M'DH inhibitor, such as that disclosed in WO 00/25780 (assigned to Bristol-Myers Squibb); or mycophenolate mofetil [see A.C. Allison and E.M. Eugui, Agents Action, 44 (Suppl.): 165 (1993)].
For the treatment of HCV infection, the compounds of the present invention may also be administered in combination with the antiviral agent amantadine (1-aminoadamantane) [for a comprehensive description of this agent, see J. I~irschbaum, Anal. Profiles Dru Subs. 12: 1-36 (1983)].
The compounds of the present invention may also be combined for the treatment of HCV infection with antiviral 2'-C-branched ribonucleosides disclosed in R. E. Harry-O'kuru, et al., J. Ors. Chem., 62: 1754-1759 (1997); M. S. Wolfe, et al., Tetrahedron Lett., 36: 7611-7614 (1995); U.S. Patent No. 3,480,613 (Nov. 25, 1969);

International Publication Number WO 01/90121 (29 November 2001); International Publication Number WO 01/92282 (6 December 2001); and International Publication Number WO 02/32920 (25 April 2002); the contents of each of which are incorporated by reference in their entirety. Such 2'-C-branched ribonucleosides include, but are not limited to, 2'-C-methyl-cytidine, 2'-C-methyl-uridine, 2'-C-methyl-adenosine, 2'-C-methyl-guanosine, and 9-(2-C-methyl-(3-D-ribofuranosyl)-2,6-diaminopurine.
By "pharmaceutically acceptable" is meant that the carrier, diluent, or excipient must be compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
Also included within the present invention are pharmaceutical compositions comprising the carbocyclic nucleoside compounds and derivatives thereof of the present invention in association with a pharmaceutically acceptable Garner. Another example of the invention is a pharmaceutical composition made by combining any of the compounds described above and a pharmaceutically acceptable carrier. Another illustration of the invention is a process for making a pharmaceutical composition comprising combining any of the compounds described above and a pharmaceutically acceptable carrier.
Also included within the present invention are pharmaceutical compositions useful for inhibiting RNA-dependent RNA viral polymerase in particular HCV NSSB polymerase comprising an effective amount of a compound of the present invention and a pharmaceutically acceptable carrier.
Pharmaceutical compositions useful for treating RNA-dependent RNA viral infection in particular HCV infection are also encompassed by the present invention as well as a method of inhibiting RNA-dependent RNA viral polymerise in particular HCV NSSB
polymerise and a method of treating RNA-dependent viral replication and in particular HCV replication. Additionally, the present invention is directed to a pharmaceutical composition comprising a therapeutically effective amount of a compound of the present invention in combination with a therapeutically effective amount of another agent active against RNA-dependent RNA virus and in particular against HCV. Agents active against HCV include, but are not limited to, ribavirin, levovirin, viramidine, thymosin alpha-1, an inhibitor of HCV NS3 serine protease, interferon-a, pegylated interferon-a (peginterferon-a), a combination of interferon-a and ribavirin, a combination of peginterferon-a and ribavirin, a combination of interferon-a and levovirin, and a combination of peginterferon-a and levovirin.

W terferon-a includes, but is not limited to, recombinant interferon-a2a (such as Roferon interferon available from Hoffmann-LaRoche, Nutley, NJ), interferon-a2b (such as Intron-A interferon available from Schering Corp., Kenilworth, NJ), a consensus interferon, and a purified interferon-a product. For a discussion of ribavirin and its activity against HCV, see J.O. Saunders and S.A. Raybuck, "Inosine Monophosphate Dehydrogenase: Consideration of Structure, Kinetics, and Therapeutic Potential," Ann. Rep. Med. Chem., 35: 201-210 (2000).
Another aspect of the present invention provides for the use of the carbocyclic nucleoside compounds and derivatives thereof and their pharmaceutical compositions for the manufacture of a medicament for the inhibition of RNA-dependent RNA viral replication, in particular HCV replication, and/or the treatment of RNA-dependent RNA viral infection, in particular HCV infection. Yet a further aspect of the present invention provides for the carbocyclic nucleoside compounds and derivatives thereof and their pharmaceutical compositions for use as a medicament for the inhibition of RNA-dependent RNA viral replication, in particular HCV replication, and/or for the treatment of RNA-dependent RNA viral infection, in particular HCV infection.
The pharmaceutical compositions of the present invention comprise a compound of structural formula I as an active ingredient or a pharmaceutically acceptable salt thereof, and may also contain a pharmaceutically acceptable carrier and optionally other therapeutic ingredients.
The compositions include compositions suitable for oral, rectal, topical, parenteral (including subcutaneous, intramuscular, and intravenous), ocular (ophthalmic), pulmonary (nasal or buccal inhalation), or nasal administration, although the most suitable route in any given case will depend on the nature and severity of the conditions being treated and on the nature of the active ingredient.
They may be conveniently presented in unit dosage form and prepared by any of the methods well-known in the art of pharmacy.
In practical use, the compounds of structural formula I can be combined as the active ingredient in intimate admixture with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques. The carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral (including intravenous). In preparing the compositions for oral dosage form, any of the usual pharmaceutical media may be employed, such as, for example, water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents and the like in the case of oral liquid preparations, such as, for example, suspensions, elixirs and solutions; or carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents and the like in the case of oral solid preparations such as, for example, powders, hard and soft capsules and tablets, with the solid oral preparations being preferred over the liquid preparations.
Because of their ease of administration, tablets and capsules represent the most advantageous oral dosage unit form in which case solid pharmaceutical carriers are obviously employed. If desired, tablets may be coated by standard aqueous or nonaqueous techniques. Such compositions and preparations should contain at least 0.1 percent of active compound. The percentage of active compound in these compositions may, of course, be varied and may conveniently be between about 2 percent to about 60 percent of the weight of the unit. The amount of active compound in such therapeutically useful compositions is such that an effective dosage will be obtained. The active compounds can also be adnunistered intranasally as, for example, liquid drops or spray.
The tablets, pills, capsules, and the like may also contain a binder such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, lactose or saccharin. When a dosage unit form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier such as a fatty oil.
Various other materials may be present as coatings or to modify the physical form of the dosage unit. For instance, tablets may be coated with shellac, sugar or both. A syrup or elixir may contain, in addition to the active ingredient, sucrose as a sweetening agent, methyl and propylparabens as preservatives, a dye and a flavoring such as cherry or orange flavor.
Compounds of structural formula I may also be administered parenterally. Solutions or suspensions of these active compounds can be prepared in water suitably mixed with a surfactant such as hydroxy-propylcellulose.
Dispersions can also be prepared in glycerol, liquid polyethylene glycols and mixtures thereof in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.
The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases, the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g. glycerol, propylene glycol and liquid polyethylene glycol), suitable mixtures thereof, and vegetable oils.
Any suitable route of administration may be employed for providing a mammal, especially a human with an effective dosage of a compound of the present invention. For example, oral, rectal, topical, parenteral, ocular, pulmonary, nasal, and the like may be employed. Dosage forms include tablets, troches, dispersions, suspensions, solutions, capsules, creams, ointments, aerosols, and the like.
Preferably compounds of structural formula I are administered orally.
For oral administration to humans, the dosage range is 0.01 to 1000 mg/kg body weight in divided doses. In one embodiment the dosage range is 0.1 to 100 mg/kg body weight in divided doses. In another embodiment the dosage range is 0.5 to 20 mg/kg body weight in divided doses. For oral administration, the compositions are preferably provided in the form of tablets or capsules containing 1.0 to 1000 milligrams of the active ingredient, particularly, 1, 5, 10, 15, 20, 25, 50, 75, 100, 150, 200, 250, 300, 400, 500, 600, 750, 800, 900, and 1000 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated.
The effective dosage of active ingredient employed may vary depending on the particular compound employed, the mode of administration, the condition being treated and the severity of the condition being treated. Such dosage may be ascertained readily by a person skilled in the art. This dosage regimen may be adjusted to provide the optimal therapeutic response.
The compounds of the present invention contain one or more asymmetric centers and can thus occur as racemates and racemic mixtures, single enantiomers, diastereomeric mixtures and individual diastereomers. The present invention is meant to comprehend carbocyclic nucleoside compounds having the stereochemical configuration for the five-membered carbocycle depicted in the structural formula below, that is, carbocyclic nucleoside compounds in which the substituents at the positions denoted as 1 and 4 in the formula below have a cis relative configuration.

R~ X Rs The stereochemistry of the R1-R4 substituents on the cyclopentane ring of the compounds of the present invention of structural formula I above is denoted by squiggly lines which signifies that substituents R1, R~, R3 and R4 can have either the a (substituent "down") or (3 (substituent "up") configuration independently of one another.
Some of the compounds described herein contain olefinic double bonds, and unless specified otherwise, are meant to include both E and Z
geometric isomers.
Some of the compounds described herein may exist as tautomers such as keto-enol tautomers. The individual tautomers as well as mixtures thereof are encompassed with compounds of structural formula I. Example of keto-enol tautomers which are intended to be encompassed within the compounds of the present invention are illustrated below:
OH O
~ N R8~ NH
R5 R ~ I ~ 11 R50 N N~R11 X N R6 N R R~ X Rs R4 R1 R4 ",.r R1 ~~N ' ~NH
R50 X N ~W - R50 X N ~W
R~ R6 ~ R~ R6 Compounds of structural formula I may be separated into their individual diastereoisomers by, for example, fractional crystallization from a suitable solvent, for example methanol or ethyl acetate or a mixture thereof, or via chiral chromatography using an optically active stationary phase.
Alternatively, any stereoisomer of a compound of the structural formula I may be obtained by stereospecific synthesis using optically pure starting materials or reagents of known configuration.
The compounds of the present invention may be administered in the form of a pharmaceutically acceptable salt. The term "pharmaceutically acceptable salt" refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids including inorganic or organic bases and inorganic or organic acids.
Salts of basic compounds encompassed within the term "pharmaceutically acceptable salt"
refer to non-toxic salts of the compounds of this invention which are generally prepared by reacting the free base with a suitable organic or inorganic acid.
Representative salts of basic compounds of the present invention include, but are not limited to, the following: acetate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, camsylate, carbonate, chloride, clavulanate, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, iodide, isothionate, lactate, lactobionate, laurate, malate, maleate, mandelate, mesylate, methylbromide, methylnitrate, methylsulfate, mucate, napsylate, nitrate, N-methylglucamine ammonium salt, oleate, oxalate, pamoate (embonate), palmitate, pantothenate, phosphateldiphosphate, polygalacturonate, salicylate, stearate, sulfate, subacetate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide and valerate. Furthermore, where the compounds of the invention carry an acidic moiety, suitable pharmaceutically acceptable salts thereof include, but are not limited to, salts derived from inorganic bases including aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic, mangamous, potassium, sodium, zinc, and the like. Particularly preferred are the ammonium, calcium, magnesium, potassium, and sodium salts. Salts derived from pharmaceutically acceptable organic non-toxic bases include salts of primary, secondary, and tertiary amines, cyclic amines, and basic ion-exchange resins, such as arginine, betaine, caffeine, choline, N,N-dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine, and the like.
Also, in the case of a carboxylic acid (-COOH) or alcohol group being present in the compounds of the present invention, pharmaceutically acceptable esters of carboxylic acid derivatives, such as methyl, ethyl, or pivaloyloxymethyl, or acyl derivatives of alcohols, such as acetate, octanoate, or maleate, can be employed.
Included are those esters and acyl groups lcnown in the art for modifying the solubility or hydrolysis characteristics for use as sustained-release or prodrug formulations.
Preparation of the Compounds of the Invention The compounds of the present invention can be prepared following modifications of procedures described by Bindu Madhavan et al. in J. Org.
Chem., 51:
1287-1293 (1986) and J. Med. Chem., 31: 1798-1804 (1988) as well as synthetic methodologies well-established in the practice of nucleoside and nucleotide chemistry, as described in "Chemistry of Nucleosides and Nucleotides," L.B.
Townsend, ed., Vols. 1-3, Plenum Press, 1988, which is incorporated by reference herein in its entirety.
Abbreviations TJsed in the Description of the Preparation of the Compounds of the Present Invention:

BC13 Boron trichloride BOM-Cl Benzyl chloromethyl ether BuLi n-Butyl lithium CH2C1~ Dichloromethane DCC 1,3-Dicyclohexylcarbodiimide DIPEA N,N-Diisopropylethylamine DMA N,N-Dimethylacetamide DMF N,N-Dimethylformamide DMSO Dimethyl sulfoxide ESMS Electrospray mass spectrum EtOAc Ethyl acetate HPLC High-performance liquid chromatography LiBH4 Lithium borohydride LAH Lithium aluminum hydride MCPBA meta-Chloroperbenzoic acid MMT p-Methoxyphenyldiphenylmethyl (p-anisyldiphenylmethyl) MS Mass spectral NMR Nuclear magnetic resonance POC13 Phosphorus oxychloride TDA-1 Tris[2-(2-methoxyethoxy)ethyl]amine THF Tetrahydrofuran TIPDS (1,1,3,3-Tetraisopropyldisiloxanylidene) TLC Thin-layer chromatography TREATHF Triethylamine trihydrofluoride Reaction Schemes A-B illustrate the methods employed in the synthesis of the compounds of the present invention of structural formula I.
All substituents are as defined above unless indicated otherwise.
A representative general method for the preparation of compounds of the present invention wherein X is C=CH2 is outlined in Scheme A below. This Scheme illustrates the synthesis of compounds of the present invention of structural formula A-5. The starting material is the known oxirane of structural formula A-1, whose synthesis has been described in J. Med. Chem., 31: 1798-1804 (1988). The carbocyclic "nucleosidic" linkage is constructed by opening of the oxirane in A-1 with the metal salt (such as lithium, sodium, or potassium) of an appropriately substituted purine or 7-deaza-purine A-6, such as an appropriately substituted 4-halo-1H-pynolo[2,3-el]pyrimidine, which can be generated ifa situ by treatment with an alkali hydride (such as sodium hydride), an alkali hydroxide (such as potassium hydroxide), an alkali carbonate (such as potassium carbonate), or an alkali hexamethyldisilazide (such as NaHMDS) in a suitable anhydrous organic solvent, such as acetonitrile, tetrahydrofuran, 1-methyl-2-pyrrolidinone, N,N-dimethylformamide (DMF) or N,N-dimethylacetamide (DMA). The ring-opening reaction can be catalyzed by using a phase-transfer catalyst, such as TDA-1 or triethylbenzylammonium chloride, in a two-phase system (solid-liquid or liquid-liquid). The cyclopentanol hydroxyl group in A-2 is then oxidized with a suitable oxidizing agent, such as a chromium trioxide or chromate reagent, Dess-Martin periodinane, or by Swern oxidation, to afford a cyclopentanone of structural formula A-3. Addition of a Grignard reagent, such as an alkyl, alkenyl, or alkynyl magnesium halide (for example, MeMgBr, EtMgBr, vinylMgBr, allylMgSr, and ethynylMgBr) or an alkyl, alkenyl, or alkynyl lithium, such as MeLi, across the carbonyl double bond of A-3 in a suitable organic solvent, such as tetrahydrofuran, diethyl ether, and the like, affords the tertiary cyclopentanol of structural formula A-4. The optional protecting groups in the protected carbocyclic nucleoside of structural formula A-4 are then cleaved following established deprotection methodologies, such as those described in T.W. Greene and P.G.M.
Wuts, "Protective Groups in Organic Synthesis," 3rd ed., John Wiley & Sons, 1999.
Optional introduction of an amino group at the 4-position of the 7-deaza-purine nucleus (or 6-position of a purine nucleus) is effected by treatment of a 4-halo intermediate A-5 (Z = Cl, Br, or I) with the appropriate amine, such as alcoholic ammonia or liquid ammonia, to generate a primary amine at the C-4 position (-NHZ), an alkylamine to generate a secondary amine (-NHR), or a dialkylamine to generate a tertiary amine (-NRR'). A 7H-pyrrolo[2,3-d]pyrimidin-4.(3H)one or 1,9-dihydro-purin-6-one compound may be derived by hydrolysis of A-5 (Z = Cl, Br, or I) with aqueous base, such as aqueous sodium hydroxide. Alcoholysis (such as methanolysis) of A-5 (Z = Cl, Br, or I) affords a C-4 alkoxide (-OR), whereas treatment with an alkyl mercaptide affords a C-4 alkylthio (-SR) derivative. Subsequent chemical manipulations well-known to practitioners of ordinary skill in the art of organic/medicinal chemistry may be required to attain the desired compounds of the present invention.

Scheme A
Z
Z
~N Y ~N
Bn0 N N'/\ R11 I
M Bn0 N N R1~
v0 A_6 ~O~
M = Li, Na, or K
MMT'O
A-1 Z = CI, Br, or I MMT~~ OH

Bn = benzyl MMT = p-anisyldiphenylmethyl Z Z
Y ~N ~Y I ~N
Bn0 ~N I ~ 11 Bn0 N
N R N R
Ri MgCI, Br or l R1 MMT~O ~ or RiLi MMT~O OH

R1o ~Y I ~ N
1. remove Bn and MMT
protecting groups HO N N~R11 2. optional displacement R1 or hydrolysis of Z
HO OH

A representative general method for the preparation of compounds of the present invention wherein X is CH2 is outlined in Scheme B below. This Scheme illustrates the synthesis of compounds of the present invention of structural formula B-7. A useful starting material is the aminocyclopentanetriol of structural formula B-2, which is prepared from commercially available (1R)-(-)-2-azabicyclo[2.2.1]hept-5-en-3-one (B-) in a similar fashion as that described in J. Or..g Chem., 46:

(1981) for the preparation of the corresponding racemic form. Elaboration of the amino functionality in B-2 into a substituted purine or 7-deaza-purine is carried out by methods analogous to those described in J. Med. Chem., 27: 534 (1984); J. Ors.
Chem., 51: 1289 (1986); and J. Med. Chem., 31: 1798 (1988); and references cited therein. The 1,3-diol in the derived intermediate B-3 is protected in the form of its (1,1,3,3-tetraisopropyldisiloxanylidene) (TIPDS) derivative B-4. The cyclopentanol hydroxyl group in B-4 is then oxidized with a suitable oxidizing agent, such as a chromium trioxide or chromate reagent, Dess-Martin periodinane, or by Swern oxidation, to afford a cyclopentanone of structural formula B-5. Addition of a Grignard reagent, such as an alkyl, alkenyl, or alkynyl magnesium halide (for example, MeMgBr, EtMgBr, vinylMgBr, allylMgBr, and ethynylMgBr) or an alkyl, alkenyl, or alkynyl lithium, such as MeLi, across the carbonyl double bond of B-5 in a suitable organic solvent, such as tetrahydrofuran, diethyl ether, and the like, affords the tertiary cyclopentanol of structural formula B-6. The T1PDS protecting group in the protected carbocyclic nucleoside of structural formula B-6 is then cleaved following established deprotection methodologies, such as by treatment with tetrabutylammonium fluoride in THF or triethylamine dihydrogen fluoride in THF.
Optional introduction of an amino group at the 4-position of the 7-deaza-purine nucleus (or 6-position of a purine nucleus) is effected by treatment of a 4-halo intermediate B-7 (Z = Cl, Br, or I) with the appropriate amine, such as alcoholic ammonia or liquid ammonia, to generate a primary amine at the C-4 position (-NHZ), an alkylamine to generate a secondary amine (-NHR), or a dialkylamine to generate a tertiary amine (-NRR'). A 7H-pyrrolo[2,3-d]pyrimidin-4(3H)one or 1,9-dihydro-purin-6-one compound may be derived by hydrolysis of B-7 (Z = Cl, Br, or I) with aqueous base, such as aqueous sodium hydroxide. Alcoholysis (such as methanolysis) of B-7 (Z = Cl, Br, or I) affords a C-4 alkoxide (-OR), whereas treatment with an alkyl mercaptide affords a C-4 alkylthio (-SR) derivative. Subsequent chemical manipulations well-known to practitioners of ordinary skill in the art of organiclmedicinal chemistry may be required to attain the desired compounds of the present invention. Mixtures of diastereoisomers at the stereogenic tertiary alcohol center in B-7 may be resolved by chromatographic methods, such as HPLC on a suitable solid support.
Scheme B
O HO NH2.HC1 NH
purine ring construction HO OH

Z
Z
~N
~N
N ~ ~ 11 p I N N R11 HO N R TI P SC 2, ~ O
pyridine Si /
v -O' ~~OH
HO OH O~Si B-3 ~ ~ B-4 Z
//Y ~ ~ N
\N N~R1~
O
Si R1 MgCI, Br, or I
O~Si-O O R1 Li Z
/j ~ ~ N
\N N~R~1 O 1. remove protecting groups Si R1 2. optional displacement or O~Si~O OH h drol sis of Z
Y Y

Z
~N
HO \N N"R11 HO' OH

The examples below provide citations to literature publications, which contain details for the preparation of intermediates employed in the preparation of final compounds of the present invention. The compounds of the present invention were prepared according to procedures detailed in the following examples. The examples are not intended to be limitations on the scope of the instant invention in any way, and they should not be so construed. Those skilled in the art of organic synthesis will readily appreciate that known variations of the conditions and processes of the following preparative procedures can be used to prepare these and other compounds of the present invention. All temperatures are degrees Celsius unless otherwise noted.

Scheme 1 TI OBn Bn0 1. photochemical O
H
BOM-C_I \ / 102, Rose Bengal 2. thiourea OH

Bn0 Bn0 MCPBA ~ MMT-CI H IO Moffatt -T O ~O oxidation' OH MMT'O 1-5 CI
O ~ ~N
Bn0 Bn0 O MePPh3+B~ O H N NH2 BuLi MMT'O 1-6 MMT'O 1-~ NaH
CI CI
~~ \~N . /
Bn0 N' \N' \NH2 BnO N~N~ NH2 Dess-Martin periodinane MMT~O OH MMT'O O
1_8 1-99 CI
~N
Bn0 N'\
N NH2 °
1. 80 /° Ac0 H
MeMgBr, THF Me MMT~O OH 2. BCI3 CI O
N
HO N~ ~ H HO N N~ NH2 N N 2 4N NaOH, dioxane, reflux Me Me -HO OH HO OH

(~)-2-Amino-7-[(1[3 2aOH 3a 4(3 -2,3-dihydrox -y 4-hydroxymethyl-2-methyl-5-methxlene-c~pentyll-3 7-dihydro-4H-pyrrolo f 2,3-dlpyrimidin-4-one (1-12) Step A: Vila 2j3 3a)-2-(Benzyloxymetlryl)-c~pent-4-ene-1,3-diol (1-3) Compound 1-33 was synthesized by modification of the procedure of Bindu Madhavan, G.V. et al., in J. Org. Clzer~z. 51: 1287-1293 (1986). Benzyl chloromethyl ether (BOM-Cl) (90%, 23.8 mL, 154.61 mmol) was added dropwise to a vigorously stirred suspension of cyclopentadienyl thallium (50 g, 185.53 mmol) in 50 mL of anhydrous diethyl ether at -20 °C. The resulting mixture was then stirred at -°C for 24 h. The mixture was filtered through a fritted funnel pre-cooled to -20 °C
15 into a pre-cooled round-bottom flask. The excess benzyl chloromethyl ether and the solvent were removed by evaporation under diminished pressure at -10 °C. The residue was dissolved in pre-cooled (-20 °C) methanol (100 mL). The resulting solution was added to a solution of Rose Bengal (316 mg), sodium acetate (732 mg), and thiourea (13.26 g) in 500 mL of methanol which had been pre-saturated with oxygen and cooled to -10 °C. The reaction vessel was illuminated with two 100-watt flood lamps and stirred at -10 °C for 24 h with continuous bubbling of oxygen. The solvent was then removed by evaporation under diminished pressure and the residue taken up in ethyl acetate (1500 mL). The ethyl acetate solution was washed twice with water (1000 mL) and dried over anhydrous sodium sulfate. The solvent was removed by evaporation under diminished pressure and the residue purified by flash chromatography on silica gel (first using a 7:2:1 dichloromethane/acetone/hexane system and then 2:1 ethyl acetate/hexane system as eluant). The fractions containing the product were concentrated under diminished pressure to give the title compound 1-33 (7 g), whose proton and C-13 NMR spectral data were identical to those given in the Bindu Madhavan publication.
Step B: (la 2a 3~3 4a Sa)-3-(Benzz~ymethyl)-6-oxabic~[3.1.0]hexane-2,4-diol 1-4 Compound 1-33 from Step A (2.0 g, 9.1 mmol) was dissolved in 50 mL
of dichloromethane and cooled to 0 °C. To this was added rneta-chloroperoxybenzoic acid (MCPBA) (77%, 3.5 g, 15.64 mmol) in portions. The resulting solution was stirred at room temperature for 2 d at which point the product and meta-chlorobenzoic acid precipitated out. The solvent was removed by evaporation under diminished pressure, and the resulting crude product was purified by flash chromatography on silica gel using 3:1 ethyl acetate/hexanes as eluant to afford the title compound 1-44 (2.15 g), whose proton and C-13 NMR spectral data were identical to those given in J.
Org. Cherr2. 51: 1287-1293 (1986).
St~ ep C: (~l-(la 2a 3~i 4a 5a)-(3-(Benzyloxymeth~)-4-(p-anis~diphenylmethoxy)-6-oxabicyclof3.l.Olhexan-2-of (1-5) A solution of 1-44 (260 mg, 1.1 mmol) and p-anisylchloro-diphenylmethane (460 mg, 1.49 mmol) in anhydrous pyridine (6.5 mL) was stirred under argon at room temperature for 2 d. Excess pyridine was removed by evaporation under diminished pressure. The residue was taken up in ethyl acetate (30 mL), washed twice with water (20 mL), twice with saturated sodium bicarbonate solution, and dried over anhydrous sodium sulfate. The solvent was removed by evaporation under diminished pressure and the residue purified by flash chromatography on silica gel using 4:1 hexane/ethyl acetate as eluant to give the title compound 1-55 (260 mg), whose proton and carbon-13 NMR spectral data matched those given in Bindu Madhavan, G.V. et al., J. Med. Chem., 31: 1798-1804 (1988).
Step D: (~)-(la 3~3 4a 5a)-3=(Benzyloxymethyl)-4-(p-anisyldiphenylmethoxy)-6-oxa-bicyclo f 3.l.Olhexan-2-one (1-6) Methylphosphonic acid (7 mg, 0.05 mmol) was added to a solution of 1-55 (260 mg, 0.52 mmol) and 1,3-dicyclohexylcarbodiimide (420 mg, 2.03 mmol) in methylsulfoxide (2.5 mL) cooled to 0 °C. After the mixture had stirred at room temperature for 16 h, a solution of oxalic acid (335 mg in 3.35 mL of water) was added and stirring was continued for an additional 2 h. The mixture was filtered and the filtrate diluted with ethyl acetate (30 mL). The resulting solution was extracted three times with brine (10 mL). The ethyl acetate layer was dried over anhydrous sodium sulfate and evaporated under diminished pressure. The residue was purified by flash chromatography on silica gel using 4:1 hexane/ethyl acetate as eluant to give the title compound 1-66 (135 mg), whose proton and carbon-13 NMR spectral data matched those given in Bindu Madhavan, G.V. et al., J. Med. Chem., 31: 1798-(1988).
Step E: (~)-(la 2a 3 j3 5a)-3-(Benzyloxymethyl)-2-(~p-anisyldiphenylmethoxy)-4-met~lene-6-oxa-bicyclo(3.l.Olhexane (1-7) To a solution of methyltriphenylphosphonium bromide (193 mg, 0.54 mmol) in anhydrous THF (2.66 mL) at -78 °C under argon was added n-butyllithium (0.375 mL of a 1.6 M solution in hexanes, 0.6 mmol). The solution was allowed to come to room temperature, stirred for 20 min, and then re-cooled to -78 °C. To this mixture was added a solution of 1-66 (135 mg, 0.27 mmol) in 1.5 mL THF. The resulting solution was allowed to come to room temperature and stirred overnight.
The reaction mixture was diluted with water (30 mL) and extracted three times with diethyl ether (60 mL). The combined ether extracts were dried over anhydrous sodium sulfate and evaporated under diminished pressure. The residue was purified by flash chromatography on silica gel using 5:1 hexanes/ethyl acetate as eluant to give title compound 1-77 (130 mg), whose proton and carbon-13 NMR spectral data matched those given in Bindu Madhavan, G.V. et al., J. Med. Chem. 1988, 31, 1804.

Step F: (~)-(la 2~3 4.~i 5al-2~2-Amino-4-chloro-7H-pyrrolof2,3-dlp~rimidin-7--4-(benzyloxymethyl)-5-(p-anis~diphenylmethoxy)-3-methylene-~clopentanol (1-8) Sodium hydride (14.4 mg of a 60% suspension, 0.36 mmol) and 2-amino-4-chloro-7H pyrrolo[2,3-d]pyrimidine (62 mg, 0.36 mmol) were dissolved in anhydrous DMF (5 mL) and stirred at 120 °C for 15 min. A solution of 1-77 in 1 mL
DMF was added and the reaction was stirred overnight under argon at 120 °C. The solvent was evaporated under diminished pressure and the residue was talcen up in dichloromethane (20 mL). The organic layer was washed twice with water (15 mL) and dried over anhydrous Na2S04. The solvent was removed by evaporation under diminished pressure and the residue purified by flash chromatography on silica gel using 1:1 hexanes/EtOAc as eluant to give 25 mg of title compound 1-88 as a white foam.
1H NMR (CDC13 ): ~ 7.2-7.6 (m, 17H), 6.8 (m, 4H), 6.2 (d, 1H), 5.49 (d, 1H), 4.27 (s, 2H), 4.08 (d, 1H), 3.75 (s, 3H), 3.4 (br, 2H), 3.2 (br, 1H).
Step G: (~)-(~ 4~3 5a)-2-(2-Amino-4-chloro-7H-pyrrolo(2,3-dlpyrimidin-7-yl)-4-(benz ~~lo~methyl)-5-(p-anisyldiphenylmethoxy)-3-meth l~nec~pentanone ( 1-9) Compound 1-88 (1 eq) is oxidized by dissolving it in anhydrous dichloromethane and adding the solution to an ice-cold suspension of Dess Martin periodinane (4 eq) in anhydrous dichloromethane under argon. The solution after stirring at room temperature for 4 d is diluted with ethyl acetate and poured into a solution of sodium thiosulfate in saturated sodium bicarbonate solution. The organic layer is separated and dried over anhydrous Na2S04. The residue is purified by flash chromatography on a silica gel column to give the title compound 1-99.
Step H: ~~)-(laOH 2J3 4~3 5a)-2-(2-Amino-4-chloro-7H pyrrolof2 3-dlpyrimidin-7-~)-(4-benzyloxymeth ly )-5-(p-anisyldiphenylmethoxy)-1-methyl-3-methylenec~pentanol (1-10) Compound 1-99 from Step G is dissolved in anhydrous THF and the solution is added to a solution of methylmagnesium bromide (4 eq) in anhydrous THF
at -78 °C. The resulting mixture is stirred overnight at -70 °C
to -50 °C. The reaction mixture is quenched with saturated NH4Cl solution and the resulting slurry filtered through a pad of celite. The residue is washed with ethyl acetate and the combined filtrate and washings are transferred to a separatory funnel. After separating the organic layer, it is washed with saturated aqueous NHq.CI solution followed by water and then brine. After drying the organic layer over anhydrous Na2SO4, the filtrate is evaporated under diminished pressure followed by purification of the residue by flash chromatography on silica gel to furnish the title compound 1-10.
Step I: ~~)-(laOH 2a 3~3 5~3)-5-(2-Amino-4-chloro-7H-pyrrolof2 3-dlpyrimidin-7 yl)-3-(benzyloxymeth~)-1-meth-4-methylenec~pentane-1,2-diol This compound is prepared by dissolving compound 1-10 in 80°Io acetic acid and stirring overnight. The solvent is removed by evaporation under diminished pressure and the residue coevaporated twice with toluene. The residue is purified by chromatography on silica gel to furnish the title compound.
Step J: (~)-(laOH 2a 3(3 5 i~-5-(2-Amino-4-chloro-7H-pyrrolof2,3-dlpyrimidin-7-yl)-3-(hydroxymethyl)-1-methyl-4-methylenecyclopentane-1,2-diol (1-11) This compound is prepared by treating a solution of the compound from Step I in anhydrous dichloromethane with boron trichloride at -70 °C for several h. The reaction is quenched with ammonia in methanol and the solvents are removed by evaporation under diminished pressure. Purification of the residue on a silica gel column affords the desired product 1-11.
Step I~: (~)-2-Amino-7-[(1 Q 2aOH 3a 4~3 -'~ 3-dihydrox -y 4-hydroxymethyl-2-methyl-5-meth len~e-c~~entyll-3 7-dihydro-4H-pyrrolo~2,3-dlpyrimidin-4-one (1-12) The title compound is obtained from compound 1-11 by dissolving it in 1,4-dioxane and treating the solution with 4N NaOH at reflux temperature for several h. After cooling to room temperature, the reaction mixture is neutralized with 4N HCI, the mixture evaporated and the crude product purified by silica gel chromatography.

Scheme 2 CI CI
Bn0 / I ~ N / I ~ N
N~N~ Bn0 N/\NJ
H
MMT'O 1-~ NaH
MMT~O OH

CI CI
N
Bn0 N~N~ Bn0 N~N
Dess-Martin _ MeMgBr_ periodinane THF Me MMT~O O MMT~O OH

N ~ J
HO N
1. 80% AcOH HO N N N
liquid NH3 2. BC13 Me Me HO OH HO OH

(~)-(laOH 2a 3[3 5~3)-5-(4-Amino-7H-~yrrolof2 3-dlpyrimidin-7-yl)-3-hydroxymethyl-1-methyl-4- methylenec~~entane-1 2-diol (2-5) Step A: ~~)-(la 2a 3~~3 5~3)-3 ~Benzylox~methyl)-5-(4-chloro-7H pyrrolo~2,3-dl~yrimidin-7-yl)-2-(p-anis~d~henylmethox~-4-methylenec~clopentanol (2-1) Sodium hydride (3 eq) and 4-chloro-7H pyrrolo[2,3-d]pyrimidine (3 eq) are dissolved in anhydrous DMF and stirred at 120 °C for 15 min. A
solution of 1-77 in anhydrous DMF is added and the reaction stirred overnight under argon atmosphere at 120 °C. The solvent is removed in vacuo and the residue taken up in dichloromethane. The organic layer is then washed with water and dried over anhydrous Na2SOq.. After removing the solvent by evaporation under diminished pressure, the residue is purified by flash chromatography on silica gel to give the title compound 2-11.
Step B: (~)-(2a 3~ 5(3)-3-(Benzyloxymethyl)-5-(4-chloro-7H-pyrrolof2,3 dl~~rimidin-7-~-2-(p-anisyldiphenylmethoxy)-4 meth~enecyclopentanone (2-2) The product from Step A is processed according to the procedure detailed in Step G of Example 1 to give the title compound.
Step C: ~~)-(laOH 2a 3J3 5~3)-3-(Benz~ymeth~)-5-(4-chloro-7H-pyrrolof2 3-dl~yrimidin-7-~ -) 2-(p-anis~phenylmethoxy)-1-methyl-4-meth l~yclopentanol (2-3) The product from Step B is processed according to the procedure detailed in Step H of Example 1 to give the title compound.
Step D: (~)-(laOH 2a 3~3 5~3)-3-(Benzyloxymethyl~4-chloro-7H-~yrrolof2 3-dlpyrimidin-7 yl)-1-methyl-4-methylenecyclopentane-1,2-diol This compound is obtained from the product of Step C by utilizing a similar procedure described in Step I of Example 1 to give the title compound.
Step E: (~)-(laOH 2a~3 5~3)-5-(4-Chloro-7H-p~rrolof2 3-dlpyrimidin-7-yl)-3-~hydrox~h~l)-1-methyl-4-methylenec~lopentane-1,2-diol (2-4) This compound is obtained from the product of Step D by utilizing a similar procedure described in Step J of Example 1.

Step F: ~~)-(laOH 2a 3j3 5~3)-5-(4-Amino-7H-pyrrolof2 3-dlpyrimidin-7-yl)-3-(hydroxymet~l)-1-methyl-4-methylenecyclopentane-1,2-diol (2-5) The title compound is obtained by dissolving 2-44 in liquid ammonia and heating it at 100 °C in a steel bomb overnight. After evaporation, the remaining solid is washed with anhydrous THF. The solvent is then removed under reduced pressure and the residue purified by column chromatography.
Scheme 3 CI CI
N ~N
Bn0 I N
_ N N"NH2 Bn0 N N' \NH2 O H
MMT'O 1-77 NaH
MMTrO OH

CI
~N I ~ N
Bn0 N N' \NH2 Met Dess-Martin _ THF
periodinane MMT~O O

CI
~N I ~ N
Bn0 \N N' \NH2 1. 80% AcOH
Me 2. BC13 MMT~O OH

C
N ~ N ~N ~ N H
HO -N I HO N N' \NH2 N NHS 4N NaOH _ dioxane, reflux Me Me HO OH HO OH

~~)-(1Q 2aOH 3a 4(3)-2-Amino-9-f2 3-dihydroxy-4-(hydroxymethyl)-2-methyl-5-methylenecyclopentyll-1 9-dihydro-6H-purin-6-one (3-5) St_ ep A: (~)- (la 2(3 4j3 5a~ 2-(2-Amino-6-chloro-9H-purin-9-yl)-4-benzvloxvmethvl)-5-(p-anisyldi~henylmethoxy)-3-methylenecyclo~entanol (3-1) Sodium hydride (67.2 mg of a 60°70 suspension, 1.68 mmol) and 2-amino-6-chloro-purine (285 mg, 1.68 mmol) were dissolved in anhydrous DMA (5 mL) and stirred at 120 °C for 15 min. A solution of compound 1-77 (280 mg, 0.56 mmol) in 1 mL DMA was added and the reaction was stirred overnight under argon at 120 °C. The solvent was removed by evaporation under diminished pressure and the residue was taken up in dichloromethane (20 mL). The organic layer was washed twice with water (15 mL) and dried over anhydrous Na2SO4. The solvent was removed by evaporation under diminished pressure and the residue purified by flash chromatography on silica gel (1:1 hexaneslEtOAc) to give 80 mg of the title compound 3-11 as a white foam.
St. ep B: ,(~~~3 4~3 5a)-2-(2-Amino-6-chloro-9H-purin-9-yl)-4-(benzyloxymeth~l)-5-(p-anisyldiphenylmethoxy)-3-methylenec~clopentanone (3-2) Compound 3-22 is obtained by taking the compound from Step A in anhydrous dichloromethane and adding the solution to an ice-cold suspension of Dess-Martin periodinane (4 eq) in anhydrous dichloromethane under argon. After stirring the solution at room temperature for 4 d, the mixture is diluted with ethyl acetate and poured into a solution of sodium thiosulfate in saturated sodium bicarbonate solution. The organic layer is separated and dried over anhydrous sodium sulfate. After evaporation, the residue is purified by flash chromatography on silica gel.
Step C: (~)-(laOH~3 4~fi 5a)-2-(2-Amino-6-chloro-9H-purin-9-yl)-4-(benzyloxymethyl)-5-(p-anisyldiphenylmethoxy)-1-methyl-3-methylenec~clopentanol (3-3) Intermediate 3-22 is dissolved in anhydrous THF and then added to a solution of methylmagnesium bromide (4 eq) in anhydrous THF at -78 °C.
The resulting mixture after stirring overnight at -70 °C to -50 °C
is quenched with saturated NHq.CI solution. The resulting slurry is filtered through a celite pad. The residue on the pad is washed with ethyl acetate and the combined filtrate and washings transferred to a separatory funnel. The organic layer is washed with saturated aqueous NH4Cl solution, water, and brine. It is then dried over anhydrous Na2S04, and concentrated under diminished pressure. The residue is purified by flash chromatography to give the title compound 3-33.
Ste~D: (~)-(laOH 2a 3J3 5~3)-5-~-Amino-6-chloro-9H-purin-9-yl)-3-(~ ~~~ -yiVxymethyl)-t-methyl-4-methylenec~lopentane-1,2-diol This compound is obtained from the product of Step C by utilizing a similar procedure described in Step I of Example 1 to give the title compound.
Step E: (~)-( 1 aOH 2a 3 ~3 5 (3)-5 ~2-Amino-6-chloro-9H purin-9- 1 (hydroxymethyl)-1-methyl-4-meth l~ec~pentane-1,2-diol (3-4) This compound is obtained from the product of Step D by utilizing a similar procedure described in Step J of Example 1.
Step F: (~)- (~3 2aOH 3a 4~3)-2-Amino-9-[2 3-dihydroxy-4-(hydrox~yl)-2-methyl-5-methXlenec~o~pentyll-1 9-dihydro-6H-purin-6-one (3-5) This compound is obtained from the product of Step E by utilizing a similar procedure described in Step I~ of Example 1.

Scheme 4 O O
NH NH
Os04~ 1. aa. HCI
N O 2. MeOH/HCI
HO '~~OH

Me02C NH2.HC1 HO NH2.HC1 LiBH4 . .; ~ ' J~OH
Hp ~OH HO

St, ep A: (1R 4S 5R 6S)-5 6-Dihydroxx-2-azabicyclo f 2.2.llheptan-3-one (4-2) To a mixture of (1R)-(-)- 2-azabicyclo[2.2.1]hept-5-en-3-one 4-1) (10.9 g, 99.8 mmol) in dioxane was added 4-methylmorpholine-N-oxide (17.4 g, 148.52 mmol) and the reaction mixture was cooled in an ice-bath. To this solution was added osmium tetroxide (30 mL, 4% solution in water) and the mixture was stirred at room temperature for 3 h. Sodium bisufite (17.0 g) was added and the residue was filtered through celite, concentrated in vacuo and passed through a short column of silica gel using CH2C12/MeOH (95:5) as eluent to afford the title compound as colorless solid;
yield 7.5 g. The proton NMR spectral data in D20 were found to be identical to those given in J.Org.Chem. 46: 3268 (1981).
Ste~B: Methyl (1S 2R 3S 4R)-4-amino-2,3-dihydroxy-~clopentanecarboxylate hydrochloride (4-3) This compound was prepared following the procedure described by B.
L. Kam and N. J. Oppenheimer in J. Ors. Chem. 46: 3268 (1981) for the corresponding racemic compound.
Step C: (1R 2S 3R 5R)-3-Amino-5-(hydro~meth~yclopentane-1,2-diol hydrochloride (4-4) To a mixture of 4-33 (2.1 g, 9.9 mmol) in THF (10 mL) was added lithium borohydride (0.32 g, 14.6 mmol) under cooling to ice temperature and the reaction mixture was stirred at room temperature overnight, evaporated in vacuo and treated with methanol. The mixture was cooled in an ice-bath and acidified with O.1N
HCl. The solvent was removed in vacuo and triturated with acetone. The residual oil was dried in vacuo and used without further purification as shown in Scheme 6 in the synthesis of 6-66.
Scheme 5 O CI
N, /
H ~ ~ POC13 _ ' ~ Os04, NMO
\N OH diethylaniline N CI Na104/H~O
5_1 5-22 CI CI
N ~ I ECHO pyridinium N ~ I ~CH(OEt)2 'N CI P-toluenesulfona~, ~N CI
EtOH

Step A: 5-All-4 6-dichloropyrimidine (5-2) A mixture of 5-allyl-4,6-dihydroxypyrimidine (5-1) [prepared following the procedure described in J. Med. Chem., 10: 665 (1967)] (6.0 g, 39.4 mmol), diethylaniline (7.5 mL, 46 rnmol ), benzyltriethylammonium chloride (18 g, 79 mmol) and POCl3 (20 mL) in acetonitrile (100 mL) was heated at 110 °C with stirring overnight. The reaction mixture was cooled and poured onto crushed ice, and extracted with ethyl acetate. The organic layer was washed with water (20 mL) dried over anhdydrous Na2S04 and concentrated to an oil which was passed through a short band of silica gel using CH2C1~ as eluent; yield 3.3 g.
1H NMR (CDCl3): 8 3.67 (m, 2H, CHI), 5.14 (m, 2H, CH2), 5.89 (m, 1H, CH), 8.66 (s, 1H, H-2).
Step B: (4 6-Dichloropyrimidine-5-yl)acetaldehyde (5-3) This compound was prepared by modification of the procedure described in J. Med. Chem.l0: 665 (1967). A solution of 5-22 (3.0 g, 15.7 mmol) in dioxane (20 mL) was stirred with 4-methylmorpholine-N-oxide (2.8 g, 24 mmol) and osmium tetroxide (4°7o solution in water, 6.2 mL) for one h. Sodium bisulfite (2.6 g) was added to the mixture and the precipitated solid was removed by filtration through celite and the filtrate was concentrated in vacuo to a solid which was dissolved in CH2C12 (50 mL). Sodium periodate on silica gel (10°70 by weight, 50 g) was added to the solution. The reaction mixture was stirred at room temperature for 10 min.
Silica gel was removed by filtration and the filtrate was washed with 5°7o aqueous sodium thiosulphate solution, dried over anhydrous Na2S04 and concentrated in vacuo.
The residue was dissolved in CH2Cl2 and passed through a short column of silica gel using 0.5% methanol/CH2Cl2 as eluent; yield 2.5 g.
1H NMR (CDC13): b 4.14 (s,lH, CH2), 8.73 (s,lH, H-2), 9.80 (s, 1H, CHO).
Step C: 4 6-Dichloro-5-(2 2-diethoxyeth~pyrimidine (5-4) This compound was prepared from 5-33 following the procedure described in J. Med. Chem., 10: 665 (1967).
1H NMR (CDC13): 8 1.54 (2t, 6H, 2CH3), 3.27 (d, 2H, J=5.8Hz, CH2) , 3.59 and 3.73 (2m, 4H, 2 x OCH2), 4.82 (t, 1H, J=5.8 Hz and 11.4Hz, CH), 8.65 (s, 1H, H-2).
Scheme 6 CI HO NH2.HC1 N ° CH(OEt)2 ~ Et3N, EtOH
N CI Hp ~OH
5-,4 4-4 CI CI
Et0 Et0 ~ \ N / J
HO HN NJ 1 N HCI, HO N N TIPDSC12 dioxane ~ pyridine n HO ~OH HO ~~OH

CI
J J
N~
O N ~ O
Moffatt _ SO, O~Si-O ~OH oxidati n 6-3 ~ ~ 6-44 CI CI
N / wN
N-~ J
O N
MeMg6r S Si OOH
O. .~~' OH ~ O~Si'O Me Si 6-5b 6-5a TREATHF
CI CI
~N ~ I ~N
N HO N N
HO N
OH
Me ; ;
HO Me HO OH
6-6a 6-6b liq. NH3 ~ ~N J
N~N~ H
HO
Me H4' ~Me HO pH
6-7a 6-7b ~l 2R 3R 5R)-5-(4-Amino-7H-~yrrolo f 2 3-dlpyrimidin-7-yl)-3-(hydroxymethyl)-1-meth~cyclopentanediol-1,2-diol (6-7a) (1 2R 3R 5R)-5-(4-Amino-7H-pyrrolof2 3-dlpyrimidin-7-yl)-3-(hydroxymethyl)-1-methylcyclopentanediol-1,2-diol (6-7b) Step A: ~1R 2S 3R 5R)-3-~ f 6-chloro-5-(2 2-diethoxyethyl)pyrimidin-4-yllamino~-5-(hydroxymeth~ cyclopentane-1,2-diol (6-1) This compound was prepared from 5-44 and 4-44 following the procedure described in J. Med. Chem., 27: 534 (1984).
St_ ep B: (1R 2S 3R 5R)-3-(4-Chloro-7H-pyrrolof2 3-dlpyri~din-7-yl)-5-(hydroxymethyl)cycloRentane-1 2-diol (6-2) To a mixture of 6-11 (1.0 g, 2.6 mmol) in dioxane (15 mL) was added 1N HCl (4 mL) and the reaction mixture was stirred at room temperature for 24 h.
The mixture was then cooled in an ice-bath and neutralized with ammonium hydroxide solution, and concentrated in vacuo. The residue was treated with EtOH
and precipitated salts were removed by filtration. The filtrate was evaporated and the residue was purified by flash chromatography over silica gel using 10%
MeOH/CH2C12 as eluent to furnish the title compound 6-22 as a colorless oil;
yield 0.42 g. The proton NMR spectrum was identical to the one reported in J. Med.
Chem.
27: 534 (1984).
Step C: (6aR 8R 9S 9aR)-8-(4-Chloro-7H=pyrrolof2 3-dlpyrimidin-7-yl)-2 2 4 4-tetraiso~propylhexahydrocycloRentafflf 1 3 5 2 4ltrioxadisilocin-9-0l 6-3 A mixture of 6-22 (0.4 g, 1.4 mmol), TIPDS-dichloride (0.52 mL) and pyridine was stirred at room temperature for 1.5 h, diluted with water and extracted with ethyl acetate (2 x 50 mL). The organic layer was washed with water and dried over Na2S04. The crude product (0.68 g) after evaporation was purified by column chromatography over silica gel using 5% MeOHlCH2C12 as eluent to furnish the title compound 6-33 as a colorless foam; yield 513 mg.
1H NMR (CDC13): 8 0.955 (m, 28H, CH(CH3)2), 2.00 and 2.24 (2 m, 3H, 4'-H and CHZ), 2.98 (d, 1H, J=3.2Hz), 3.83 and 4.03 (2m, 2H, 2H-5'), 4.32 (m, 1H, 3'-H), 4.67 (m,lH, 2'-H), 4.82 (m, 1H, 1'-H), 6.60 (d, 1H, J=3.6Hz, 5-H), 7.27 (d, 1H, 6-H), 8.56 (s, 1H, 2-H).
Step D: ~6aR 8R 9aR)-8-(4-Chloro-7H-pyrrolof2 3-dlpyrimidin-7-yl)-2,2,4,4-tetraisopro~ 1hY exahydrocyclopentafflf 1 3 5 2 4ltrioxadisilocin-9(6H)-one 6-4 A mixture of 6-33 (0.5 g, 0.98 mmol), DCC (0.563 g, 2.7 mmol) and phosphoric acid (0.045 g, 0.45 mmol) in DMSO (5.0 mL) was stirred at room temperature overnight. The residue was dissolved in a mixture of 2%
MeOH/CH2C12 and purified by column chromatography over silica gel using 2% MeOH/CH2C12 as eluent to furnish the title compound 6-44 as a colorless solid.

Step E: (6aR 8R 9S 9aR)-8-(4-Chloro-7H-pyrrolof2 3-dlpyrimidin-7-yl)-2 2 4 4-tetraisopropyl-9-methylhexahydrocyclopenta-~fl f 1 3 5 2 4ltrioxadisilocin-9-of (6-5a) and ~6aR 8R 9R 9aR)-8-(4-chloro-7H-pyTOlo~2 3-dlpyrimidin-7-yl)-2,2 4 4-tetraisopropyl-9-methylhexahydrocyclopenta-ffl~l 3 5 2 4ltrioxadisilocin-9-of (6-5b) To a cooled solution of 6-44 (0.4 g, 0.7 mmol) in toluene under argon cooled to -10 °C was added methylmagnesium bromide (3M soln.in ether, 0.5 mL, 1.4 mmol) and the reaction mixture was stirred at room temperature for 6 h. To this solution was added an additional methylmagnesium bromide (0.25 mL, 0.7 mmol) and the reaction mixture was stirred overnight. It was then cooled to 0°C and poured into ice water and extracted with ethyl acetate (3 x 50 mL). The organic layer was washed with water (2 x 15 mL) and dried over Na2S04 and concentrated to an oil (0.4 g) which was purified by column chromatography over silica gel using 1-5%
dichloromethane-acetone as eluant to furnish a-methyl isomer 6-5b (90 mg) followed by the (3-methyl isomer 6-5a (25 mg).
6-5b: 1I~TMR (CDC13): 8 1.01-1.12 (m, 28H), 1.26(s, 3H), 2.08-2.16 (m, 3H), 2.51 (s, 1H), 3.76-3.85 (m, 1H), 4.00-4.15 (m, 2H), 4.90-4.99 (m, 1H), 6.61 (d, 1H, J = 3.6 Hz), 7.46 (d, 1H, J= 3.6 Hz), 8.60 (s, 1H); ESMS (CzsH4zC1N304Siz, 540.24 M+1).
6-5a: 1H NMR (CDCl3): 8 0.78 (s, 3H), 1.01-1.18 (m, 28H), 2.30-2.36 (m, 3H), 2.87 (s, 1H), 3.86-3.92 (m, 1H), 4.04-4.18 (m, 2H), 5.01-5.10 (m, 1H), 6.61 (d, 1H, J = 3.6 Hz), 7.28 (d, 1H, J = 3.6 HZ), 8.62 (s, 1H), ESMS (Cz5H4zC1N3O4Siz, 540.24 M+1).
Step F: ~1R 2R 3R 5R)-5-(4-Chloro-7H=pyrrolof2 3-dlpyrimidin-7-yl)-3-~hydroxymeth~ -1-meth~yclopentane-1 2-diol (6-6b) To a solution of 6-5b (24 mg, 0.45 mmol) in anhydrous THF (1 mL) was added triethylamine (0.03 mL) and TREATHF (0.06 mL) and reaction mixture was stirred at room temperature overnight. It was concentrated in vacuo and co-evaporated with toluene and purified by column chromatography over silica gel using 10% MeOH in dichloromethane as eluent to furnish 6-6b (13 mg).
1H NMR (DMSO-d6): b 0.99 (s, 3H), 1.89-2.05 (m, 2H), 2.04-2.11 (m, 1H), 3.44-3.52 (m, 2H), 3.55-3.60 (m,lH), 4.75 (t,lH), 4.81 (bs, 1H), 5.00-5.07 (m, 2H), 6.62 (d,lH, J = 3.6 Hz), 7.80 (d,lH, J = 3.6 HZ), 8.60 (s,lH); ESMS (C13HI~C1N3O3, 298.0 M+1).
~1S,2R,3R,5R)-5-(4-Chloro-7H pyrrolo f 2 3-dlRyrimidin-7-yl)-3-~hydrox~methyl)-I-methylcyclopentane-12-diol (6-6a) Compound 6-5a (25 mg) was treated with TREATHF under identical experimental conditions as with 6-5b to furnish 6-6a in 60% yield.
1HNMR (DMSO-d~) ~ 0.57 (s, 3H), 1.9-1.97 (m,. IH), 2.05-2.12 (m, 1H), 2.36-2.44 (m, 1H), 3.61-3.65 (m, 3H), 4.52 (bs, 1H), 4.73 (d, 1H, J = 6.8 Hz), 4.80 (t, 1H), 5.03-5.07 (m, 1H), 6.65 (d, 1H, J = 3.6 Hz), 7.91 (d, 1H, J = 3.6 Hz), 8.62 (s, 1H); ESMS
(C13H1~C1N303, 298.0 M+1).
Step G: (1R,2R,3R,5R)-5-(4-Amino-7H-pyrrolof2 3-dlpyrimidin-7-yl)-3-(hydroxymethyl)-1-methylcyclopentane-12-diol (6-7b) A mixture of 6-6b (2.0 mg) and liquid ammonia is heated in a steel bomb at 120 °C overnight. The residue was purified by reverse phase HPLC to afford 6-7b.
(1S,2R,3R,5R)-5-(4-Amino-7H-pyrrolo f 2 3-dlpyrimidin-7-yl)-3-(hydroxyl)-1-methylcyclopentane-1 2-diol (6-7a) A mixture of 6-6a (2.0 mg) and liquid ammonia is heated in a steel bomb at 120 °C overnight. The residue was purified by reverse phase HPLC to afford 6-7a.
Examples 1, 2, and 3 can also prepared according to procedures depicted in Schemes 7, 8, and 9, respectively.
Scheme 7 Bn0 N3 Dess-Martin BnO Na periodinane MeMgBr, --~-MMT~O OH MMT'O O TiCl4, THF
7_1 7-22 CI
O Et N~
Bn0 N3 Bn0 NH2 1. ~ ~ pEt LAH H N N CI
Me ~ Me MMT~O OH MMT~O OH EtsN, EtOH
2. 1 N HCI, dioxane CI O
N
HO N~N~NH2 HO N N NHS
4N NaOH _ Me Me dioxane, reflux HO OH HO OH

Scheme 8 MMT~O OH 2, 1N HCI, dioxane HO OH
CI CI
N ~ OEt NH2 ~ I ~ t Bn0 ~N CI HO N N
Me Et3N, EtOH Me 7-44 8_1 ~~ N
J
N
NH3 _ ~Me heat HO OH

Scheme 9 CI
CI
~N
Bn0 NHS N ~
Bn0 HN N NH2 Me H2N N CI
MMT'O OH Me t-butanol, DIPEA
MMT'O OH

N
CI ~ ~ N2+CI- Bn0 N NH2 Me AcOH, NaOAc 10% Pd(C), MMT'O OH MeOH

CI CI
H2N / N <N ~ N
i~ 1. 80% AcOH 'N

Me 2. HC(OEt)3, Me aq. HCI
MMT'O OH 3. BC13, CH2CI2 HO OH
9_3 9-44 CI /
CI
N=N
/~
HN ~

Ci N I ~ N ~N ~ NH
HO N N"NH2 HO \N N! 'NH2 4N NaOH, Me dioxane, reflux Me HO OH HO OH
g_5 3~5 BIOLOGICAL ASSAYS
The assays employed to measure the inhibition of HCV NSSB
polymerase and HCV replication are described below.
The effectiveness of the compounds of the present invention as inhibitors of HCV NSSB RNA-dependent RNA polymerase (RdRp) was measured in the following assay.
A. Assay for Inhibition of HCV NSSB Polymerase_ This assay was used to measure the ability of the carbocyclic nucleoside derivatives of the present invention to inhibit the enzymatic activity of the RNA-dependent RNA polymerase (NSSB) of the hepatitis C virus (HCV) on a heteromeric RNA template.
Procedure:
Assay Buffer Conditions: (50 ~.L -total/reaction) mM Tris, pH 7.5 50 wM EDTA
20 5 mM DTT
2 mM MgCl2 80 mM KCl 0.4 U/~,L RNAsin (Pxomega, stock is 40 units/p.L) 0.75 ~,g t500 (a 500-nt RNA made using T7 runoff transcription with a sequence from the NS2/3 region of the hepatitis C genome) 1.6 p,g puxified hepatitis C NSSB (form with 21 amino acids C-terminally truncated) 1 ~,M A,C,U,GTP (Nucleoside triphosphate mix) [alpha-32P]-GTP or [alpha-33P]-GTP
The compounds were tested at various concentrations up to 100 ~.M
final concentration.
An appropriate volume of reaction buffer was made including enzyme and template t500. Carbocyclic nucleoside derivatives of the present invention were pipetted into the wells of a 96-well plate. A mixture of nucleoside triphosphates (NTP's), including the radiolabeled GTP, was made and pipetted into the wells of a 96-well plate. The reaction was initiated by addition of the enzyme-template reaction solution and allowed to proceed at room temperature for 1-2 h.
The reaction was quenched by addition of 20 ~,L O.SM EDTA, pH 8Ø
Blank reactions in which the quench solution was added to the NTPs prior to the addition of the reaction buffer were included.
50 ~,L of the quenched reaction were spotted onto DE81 filter disks (Whatman) and allowed to dry for 30 min. The filters were washed with 0.3 M
ammonium formate, pH 8 (150 mL/wash until the cpm in 1 mL wash is less than 100, usually 6 washes). The filters were counted in 5-mL scintillation fluid in a scintillation counter.
The percentage of inhibition was calculated according to the following equation: °IoInhibition = [1-(cpm in test reaction - cpm in blank) /
(cpm in control reaction - cpm in blank)] x 100.
Representative compounds tested in the HCV NSSB polymerase assay exhibited IC$o's less than 100 micromolar.
B. Assay for Inhibition of HCV RNA Replication:
The compounds of the present invention were also evaluated for their ability to affect the replication of Hepatitis C Virus RNA in cultured hepatoma (HuH-7) cells containing a subgenomic HCV Replicon. The details of the assay are described below. This Replicon assay is a modification of that described in V.
Lohmann, F. Korner, J-O. Koch, U. Herian, L. Theilmann, and R. Bartenschlager, "Replication of a Sub-genomic Hepatitis C Virus RNAs in a Hepatoma Cell Line,"
Science 285:110 (1999).
Protocol:
_57_ The assay was an in situ Ribonuclease protection, Scintillation Proximity based-plate assay (SPA). 10,000 - 40,000 cells were plated in 100-200 p.L
of media containing 0.8mg/mL 6418 in 96-well cytostar plates (Amersharn).
Compounds were added to cells at various concentrations up to 100 ~,M in 1 %
DMSO
at time 0 to 18 h and then cultured for 24-96 h. Cells were fixed (20 min, 10%
formalin), permeabilized (20 min, 0.25% Triton X-100/PBS) and hybridized (overnight, 50°C) with a single-stranded 33P RNA probe complementary to the (+) strand NSSB (or other genes) contained in the RNA viral genome. Cells were washed, treated with RNAse, Washed, heated to 65°C and counted in a Top-Count.
Inhibition of replication was read as a decrease in counts per minute (cpm).
Human HuH-7 hepatoma cells, which were selected to contain a subgenomic replicon, carry a cytoplasmic RNA consisting of an HCV 5' non-translated region (NTR), a neomycin selectable marker, an EMCV IRES (internal ribosome entry site), and HCV non-structural proteins NS3 through NSSB, followed by the 3' NTR.
Representative compounds tested in the replication assay exhibited ECSn's less than 100 micromolar.
The carbocyclic nucleoside derivatives of the present invention were also evaluated for cellular toxicity and anti-viral specificity in the counterscreens described below.
C. COUNTERSCREENS:
The ability of the carbocyclic nucleoside derivatives of the present invention to inhibit human DNA polymerases was measured in the following assays.
a. Inhibition of Human DNA Polymerases alpha and beta' Reaction Conditions:
50 ~uL reaction volume Reaction buffer components:
20 mM Tris-HCI, pH 7.5 200 p,g/mL bovine serum albumin 100 mM KCl 2 mM (3-mercaptoethanol mM MgCl2 1.6 ,uM dA, dG, dC, dTTP
a-33P-dATP

Enzyme and template:
0.05 mg/mL gapped fish sperm DNA template 0.01 U/,uL DNA polymerase oc or (3 10 Preparation of gapped fish sperm DNA template' Add 5 ~,L 1M MgClz to 500 ~,L activated fish sperm DNA (USB 70076);
Warm to 37°C and add 30 ,uL of 65 U/p,L of exonuclease III (GibcoBRL
18013-011);
Incubate 5 min at 37°C;
Terminate reaction by heating to 65 °C for 10 min;
Load 50-100 ~L aliquots onto Bio-spin 6 chromatography columns (Bio-Rad 732-6002) equilibrated with 20 mM Tris-HCl, pH 7.5;
Elute by centrifugation at 1,000Xg for 4 min;
Pool eluate and measure absorbance at 260 nm to determine concentration.
The DNA template was diluted into an appropriate volume of 20 mM
Tris-HCI, pH 7.5 and the enzyme was diluted into an appropriate volume of 20 mM
Tris-HCI, containing 2 mM (3-mercaptoethanol, and 100 mM KCI. Template and enzyme were pipetted into microcentrifuge tubes or a 96 well plate. Blank reactions excluding enzyme and control reactions excluding test compound were also prepared using enzyme dilution buffer and test compound solvent, respectively. The reaction was initiated with reaction buffer with components as listed above. The reaction was incubated for 1 hour at 37°C. The reaction was quenched by the addition of 20 ~tL
0.5M EDTA. 50 p,L of the quenched reaction was spotted onto Whatman DE81 filter disks and air dried. The filter disks were repeatedly washed with 150 mL 0.3M
ammonium formate, pH 8 until 1 mL of wash is < 100 cpm. The disks were washed twice with 150 mL absolute ethanol and once with 150 mL anhydrous ether, dried and counted in 5 mL scintillation fluid.

The percentage of inhibition was calculated according to the following equation: % inhibition = [1-(cpm in test reaction - cpm in blank)/(cpm in control reaction - cpm in blank)] x 100.
b Inhibition of Human DNA Polymerise Gamma The potential for inhibition of human DNA polymerise gamma was measured in reactions that included 0.5 ng/ ~,L enzyme; 10 ~,M dATP, dGTP, dCTP, and TTP; 2 ~,Ci/reaction [~-33P]-dATP, and 0.4 ~,g/q.L activated fish sperm DNA
(purchased from US Biochemical) in a buffer containing 20 mM Tris pHB, 2 mM ~3-mercaptoethanol, 50 mM I~Cl, 10 mM MgCl2, and 0.1 q,g/~,L BSA. Reactions were allowed to proceed for 1 h at 37°C and were quenched by addition of 0.5 M EDTA to a final concentration of 142 mM. Product formation was quantified by anion exchange filter binding and scintillation counting. Compounds were tested at up to 50 ~,M.
The percentage of inhibition was calculated according to the following equation: % inhibition = [1-(cpm in test reaction - cpm in blanlc)l(cpm in control reaction - cpm in blank)] x 100.
The ability of the carbocyclic nucleoside derivatives of the present invention to inhibit HIV infectivity and HIV spread was measured in the following assays.
c. HIV InfectivityAssay Assays were performed with a variant of HeLa Magi cells expressing both CXCR4 and CCRS selected for low background j3-galactosidase ((3-gal) expression. Cells were infected for 48 h, and (i-gal production from the integrated HIV-1 LTR promoter was quantified with a chemiluminescent substrate (Galactolight Plus, Tropix, Bedford, MA). Inhibitors were titrated (in duplicate) in twofold serial dilutions starting at 100 ~,M; percent inhibition at each concentration was calculated in relation to the control infection.
d. Inhibition of HIV Spread The ability of the compounds of the present invention to inhibit the spread of the human immunedeficiency virus (HIV) was measured by the method described in U.S. Patent No. 5,413,999 (May 9, 1995), and J.P.Vacca, et al., Proc.

Natl. Acad. Sci., 91: 4096-4100 (1994), which are incorporated by reference herein in their entirety.
The carbocyclic nucleoside derivatives of the present invention were also screened for cytotoxicity against cultured hepatoma (HuH-7) cells containing a subgenomic HCV Replicon in an MTS cell-based assay as described in the assay below. The HuH-7 cell line is described in H. Nakabayashi, et al., Cancer Res., 42:
3858 (1982).
e. Cytotoxicity assay:
Cell cultures were prepared in appropriate media at concentrations of approximately 1.5 x 105 cells/mL for suspension cultures in 3 day incubations and 5.0 x 104 cells/mL for adherent cultures in 3 day incubations. 99 p,L of cell culture was transferred to wells of a 96-well tissue culture treated plate, and 1 ~,L of 100-times final concentration of the test compound in DMSO was added. The plates were incubated at 37°C and 5% COZ for a specified period of time. After the incubation period, 20 ~,L of CellTiter 96 Aqueous One Solution Cell Proliferation Assay reagent (MTS) (Promega) was added to each well and the plates were incubated at 37°C and 5% C02 for an additional period of time up to 3 h. The plates were agitated to mix well and absorbance at 490 nm was read using a plate reader. A standard curve of suspension culture cells was prepared with known cell numbers just prior to the addition of MTS reagent. Metabolically active cells reduce MTS to formazan.
Formazan absorbs at 490 nm. The absorbance at 490 nm in the presence of compound was compared to absorbance in cells without any compound added.
Reference: Cory, A. H. et al., "Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture," Cancer Commun. 3: 207 (1991).
The following assays were employed to measure the activity of the compounds of the present invention against other RNA-dependent RNA viruses:
a. Determination of In Vitro Antiviral Activity of Compounds Against Rhinovirus (Cytopathic Effect Inhibition Assay:
Assay conditions are described in the article by Sidwell and Huffman, "Use of disposable microtissue culture plates for antiviral and interferon induction studies," Appl. Microbiol. 22: 797-801 (1971).

Viruses:
Rhinovirus type 2 (RV-2), strain HGP, was used with KB cells and media (0.1%
NaHC03, no antibiotics) as stated in the Sidwell and Huffman reference. The virus, obtained from the ATCC, was from a throat swab of an adult male with a mild acute febrile upper respiratory illness.
Rhinovirus type 9 (RV-9), strain 211, and rhinovirus type 14 (RV-14), strain Tow, were also obtained from the American Type Culture Collection (ATCC) in Rockville, MD. RV-9 was from human throat washings and RV-14 was from a throat swab of a young adult with upper respiratory illness. Both of these viruses were used with HeLa Ohio-1 cells (Dr. Fred Hayden, Univ. of VA) which were human cervical epitheloid carcinoma cells. MEM (Eagle's minimum essential medium) with 5% Fetal Bovine serum (FBS) and 0.1% NaHCO3 was used as the growth medium.
Antiviral test medium for all three virus types was MEM with 5% FBS, 0.1%
NaHC03, 50 ~,g gentamicin/mL, and 10 mM MgCl2.
2000 ~glmL was the highest concentration used to assay the compounds of the present invention. Virus was added to the assay plate approximately 5 min after the test compound. Proper controls were also run. Assay plates were incubated with humidified air and 5% C02 at 37°C. Cytotoxicity was monitored in the control cells microscopically for morphologic changes. Regression analysis of the virus CPE
data and the toxicity control data gave the ED50 (50% effective dose) and CC50 (50%
cytotoxic concentration). The selectivity index (SI) was calculated by the formula: SI
= CC50 = ED50.
b. Determination of In Vitro Antiviral Activity of Compounds Against Dengue Banzi, and Yellow Fever (CPE Inhibition Assay) Assay details are provided in the Sidwell and Huffman reference above.
Viruses:
Dengue virus type 2, New Guinea strain, was obtained from the Center for Disease Control. Two lines of African green monkey kidney cells were used to culture the virus (Vero) and to perform antiviral testing (MA-104). Both Yellow fever virus, 17D
strain, prepared from infected mouse brain, and Banzi virus, H 336 strain, isolated from the serum of a febrile boy in South Africa, were obtained from ATCC. Vero cells were used with both of these viruses and for assay.

Cells and Media:
MA-104 cells (BioWhittaker, Inc., Walkersville, MD) and Vero cells (ATCC) were used in Medium 199 with 5% FBS and 0.1% NaHC03 and without antibiotics.
Assay medium for dengue, yellow fever, and Banzi viruses was MEM, 2% FBS, 0.18% NaHC03 and 50 ~,g gentamicin/mL.
Antiviral testing of the compounds of the present invention was performed according to the Sidwell and Huffman reference and similar to the above rhinovirus antiviral testing. Adequate cytopathic effect (CPE) readings were achieved after 5-6 days for each of these viruses.
c. Determination of In Vitro Antiviral Activity of Compounds Against West Nile Virus (CPE Inhibition Assay) Assay details are provided in the Sidwell and Huffman reference cited above.
West Nile virus, New York isolate derived from crow brain, was obtained from the Center for Disease Control. Vero cells were grown and used as described above. Test medium was MEM, 1% FBS, 0.1°70 NaHC03 and 50 p.g gentamicin/mL.
Antiviral testing of the compounds of the present invention was performed following the methods of Sidwell and Huffman which are similar to those used to assay for rhinovirus activity. Adequate cytopathic effect (CPE) readings were achieved after 5-6 days.
d. Determination of In Vitro Antiviral Activity of Compounds Against rhino elf fever, dengue, Banzi, and West Nile Viruses (Neutral Red Uptake Assays After performing the CPE inhibition assays above, an additional cytopathic detection method was used which is described in "Microtiter Assay for Interferon: Microspectrophotornetric Quantitation of Cytopathic Effect," Appl.
Environ. Microbiol. 31: 35-38 (1976). A Model EL309 microplate reader (Bio-Tek Instruments Inc.) was used to read the assay plate. ED50's and CD50's were calculated as above.
EXAMPLE OF A PHARMACEUTICAL FORMULATION
As a specific embodiment of an oral composition of a compound of the present invention, 50 mg of the compound of Example 1 or Example 2 is formulated with sufficient finely divided lactose to provide a total amount of 580 to 590 mg to fill a size O hard gelatin capsule.
While the invention has been described and illustrated in reference to specific embodiments thereof, those skilled in the art will appreciate that various changes, modifications, and substitutions can be made therein without departing from the spirit and scope of the invention. For example, effective dosages other than the preferred doses as set forth hereinabove may be applicable as a consequence of variations in the responsiveness of the human being treated for severity of the HCV
infection. Likewise, the pharmacologic response observed may vary according to and depending upon the particular active compound selected or whether there are present pharmaceutical carriers, as well as the type of formulation and mode of administration employed, and such expected variations or differences in the results are contemplated in accordance with the objects and practices of the present invention. It is intended therefore that the invention be limited only by the scope of the claims which follow and that such claims be interpreted as broadly as is reasonable.

Claims (21)

WHAT IS CLAIMED IS:

1. A compound of structural formula I:
or a pharmaceutically acceptable salt thereof; wherein n is 0, 1, or 2;
B is X is CH2, CHF, CF2, or C=CH2;
Y is N or C-R9;
W is O or S;
R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl, wherein alkyl is unsubstituted or substituted with hydroxy, amino, C1-4 alkoxy, C1-4 alkylthio, or one to three fluorine atoms;
R2 is hydrogen, fluorine, amino, hydroxy, mercapto, C1-4 alkoxy, C1-8 alkylcarbonyloxy, or C1-4 alkyl;
R3 and R4 are each independently selected from the group consisting of hydrogen, cyano, azido, halogen, hydroxy, mercapto, amino, C1-4 alkoxy, C1-8 alkylcarbonyloxy, C2-4 alkenyl, C2-4 alkynyl, and C1-4 alkyl, wherein alkyl is unsubstituted or substituted with hydroxy, amino, C1-4 alkoxy, C1-4 alkylthio, or one to three fluorine atoms;
R5 is hydrogen, C1-10 alkylcarbonyl, P3O9H4, P2O6H3, or P(O)R13R14;

R6 and R7 are each independently hydrogen, methyl, hydroxymethyl, or fluoromethyl;
R8 is hydrogen, C1-4 alkyl, C2-4 alkynyl, halogen, cyano, carboxy, C1-4 alkyloxycarbonyl, azido, amino, C1-4 alkylamino, di(C1-4 alkyl)amino, hydroxy, C1-6 alkoxy, C1-6 alkylthio, C1-6 alkylsulfonyl, or (C1-4 alkyl)p-2 aminomethyl;
R9 is hydrogen, halogen, cyano, nitro, NHCONH2, CONR12R12, CSNR12R12, COOR12, C(=NH)NH2, hydroxy, C1-3 alkoxy, amino, C1-4 alkylamino, di(C1-4 alkyl)amino, or C1-3 alkyl, wherein alkyl is unsubstituted or substituted with one to three groups independently selected from halogen, amino, hydroxy, carboxy, and alkoxy;
R10 and R16 are each independently hydrogen, hydroxy, mercapto, halogen, C1-4 alkoxy, C1-4 alkylthio, C1-8 alkylcarbonyloxy, C3-6 cycloalkylcarbonyloxy, C1-alkyloxycarbonyloxy, C3-6 cycloalkyloxycarbonyloxy, -OCH2CH2SC(=O)C1-4 alkyl, -OCH2O(C=O)C1-4 alkyl, -OCH(C1-4 alkyl)O(C=O)C1-4 alkyl, amino, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, di(C3-6 cycloalkyl)amino, or an amino acyl residue having structural formula R11 is hydrogen, hydroxy, halogen, C1-4 alkoxy, amino, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, or di(C3-6 cycloalkylamino);
each R12 is independently hydrogen or C1-6 alkyl;
R17, R18, and R19 are each independently hydrogen or C1-6 alkyl;
R13 and R14 are each independently hydroxy, -OCH2CH2SC(=O)C1-4 alkyl, -OCH2O(C=O)OC1-4 alkyl, -NHCHMeCO2Me, -OCH(C1-4 alkyl)O(C=O)C1-4 alkyl, R15 is hydrogen, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen; and R20 is hydrogen, C1-4 alkyl, or phenyl C0-2 alkyl;
with the proviso that when B is X is CH2; Y is N; R10 is NH2; R2 and R3 are a-OH; and R4, R5, R6, R7, R8 and R11 are hydrogen, then R1 is not .beta.-methyl.

2. The compound of Claim 1 wherein B is

3. The compound of Claim 2 of structural formula II:
wherein R1 is C1-3 alkyl, wherein alkyl is unsubstituted or substituted with one to three fluorine atoms;
R2 is hydroxy, fluoro, C1-3 alkoxy, or C1-8 alkylcarbonyloxy;
R3 is hydrogen, halogen, hydroxy, amino, C1-3 alkoxy, or C1-8 alkylcarbonyloxy;
R5 is hydrogen, C1-8 alkylcarbonyl, P3O9H4, P2O6H3, or PO3H2;
R8 is hydrogen, amino, or C1-4 alkylamino; and R10 and R11 are each independently hydrogen, halogen, hydroxy, amino, C1-4 alkylamino, di(C1-4 alkyl)amino, or C3-6 cycloalkylamino;
with the proviso that when R10 is NH2, R2 and R3 are .alpha.-OH, and R5, R8, and R11 are hydrogen, then R1 is not .beta.-methyl.

4. The compound of Claim 3 wherein R1 is methyl, fluoromethyl, difluoromethyl, or trifluoromethyl;
R2 is hydroxy, fluoro, or methoxy;
R3 is hydrogen, fluoro, hydroxy, amino, or methoxy;
R5 is hydrogen or P3O9H4;
R8 is hydrogen or amino; and R10 and R11 are each independently hydrogen, fluoro, hydroxy, or amino;
with the proviso that when R10 is NH2, R2 and R3 are .alpha.-OH, and R5, R8, and R11 are hydrogen, then R1 is not .beta.-methyl.

5. The compound of Claim 2 of structural formula III:
wherein R1 is C1-3 alkyl, wherein alkyl is unsubstituted or substituted with one to three fluorine atoms;
R2 is hydroxy, fluoro, C1-3 alkoxy, or C1-8 alkylcarbonyloxy;
R3 is hydrogen, halogen, hydroxy, amino, C1-3 alkoxy, or C1-8 alkylcarbonyloxy;
R5 is hydrogen, C1-8 alkylcarbonyl, P3O9H4, P2O6H3, or PO3H2;
R8 is hydrogen, amino, or C1-4 alkylamino;
R9 is hydrogen, cyano, methyl, halogen, CONH2 or CSNH2; and R10 and R11 are each independently hydrogen, halogen, hydroxy, amino, C1-4 alkylamino, di(C1-4 alkyl)amino, or C3-8 cycloalkylamino.

6. The compound of Claim 5 wherein R1 is methyl, fluoromethyl, difluoromethyl, or trifluoromethyl;
R2 is hydroxy, fluoro, or methoxy;
R3 is hydrogen, fluoro, hydroxy, amino, or methoxy;
R5 is hydrogen or P3O9H4;
R8 is hydrogen or amino;
R9 is hydrogen, cyano, methyl, halogen, CONH2 or CSNH2; and R10 and R11 are each independently hydrogen, fluoro, hydroxy, or amino.

7. The compound of Claim 2 of structural formula IV:
wherein R1 is C1-3 alkyl, wherein alkyl is unsubstituted or substituted with one to three fluorine atoms;
R2 is hydroxy, fluoro, C1-3 alkoxy, or C1-8 alkylcarbonyloxy;
R3 is hydrogen, halogen, hydroxy, amino, C1-3 alkoxy, or C1-8 alkylcarbonyloxy;
R5 is hydrogen, C1-8 alkylcarbonyl, P3O9H4, P2O6H3, or PO3H2;
R8 is hydrogen, amino, or C1-4 alkylamino; and R10 and R11 are each independently hydrogen, halogen, hydroxy, amino, C1-4 alkylamino, di(C1-4 alkyl)amino, or C3-6 cycloalkylamino.

8. The compound of Claim 7 wherein R1 is methyl, fluoromethyl, difluoromethyl, or trifluoromethyl;
R2 is hydroxy, fluoro, or methoxy;

R3 is hydrogen, fluoro, hydroxy, amino, or methoxy;
R5 is hydrogen or P309H4;
R8 is hydrogen or amino; and R10 and R11 are each independently hydrogen, fluoro, hydroxy, or amino.

9. The compound of Claim 2 of structural formula V:
wherein R1 is C1-3 alkyl, wherein alkyl is unsubstituted or substituted with one to three fluorine atoms;
R2 is hydroxy, fluoro, C1-3 alkoxy, or C1-8 alkylcarbonyloxy;
R3 is hydrogen, halogen, hydroxy, amino, C1-3 alkoxy, or C1-8 alkylcarbonyloxy;
R5 is hydrogen, C1-8 alkylcarbonyl, P3O9H4, P2O6H3, or PO3H2;
R8 is hydrogen, amino, or C1-4 alkylamino;
R9 is hydrogen, cyano, methyl, halogen, CONH2 or CSNH2; and R10 and R11 are each independently hydrogen, halogen, hydroxy, amino, C1-4 alkylamino, di(C1-4 alkyl)amino, or C3-6 cycloalkylamino.

10. The compound of Claim 9 wherein R1 is methyl, fluoromethyl, difluoromethyl, or trifluoromethyl;
R2 is hydroxy, fluoro, or methoxy;
R3 is hydrogen, fluoro, hydroxy, amino, or methoxy;
R5 is hydrogen or P3O9H4;
R8 is hydrogen or amino;
R9 is hydrogen, cyano, methyl, halogen, CONH2 or CSNH2; and R10 and R11 are each independently hydrogen, fluoro, hydroxy, or amino.

11. The compound of Claim 2 selected from the group consisting of:

2-amino-7-[(1.beta.,2.alpha.OH,3.alpha.,4.beta.)-2,3-dihydroxy-4-hydroxymethyl-2-methyl-5-methylenecyclopentyl]-3,7-dihydro-4H-pyrrolo [2,3-d]pyrimidin-4-one;

2-amino-7-[(1R,2S,3R,4R)-2,3-dihydroxy-4-hydroxymethyl-2-methyl-5-methylenecyclopentyl]-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one;

(1.alpha.OH,2.alpha.,3.beta.3,5.beta.)-5-(4-amino-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-3-hydroxymethyl-1-methyl-4-methylenecyclopentane-1,2-diol;

(1S,2R,3R,5R)-5-(4-amino-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-3-hydroxymethyl-1-methyl-4- methylenecyclopentane-1,2-diol;

(1.beta.,2.alpha.OH,3.alpha.,4.beta.)-2-amino-9-[2, 3-dihydroxy-4-(hydroxymethyl)-2-methyl-5-methylenecyclopentyl]-1,9-dihydro-6H-purin-6-one;

2-amino-9-[(1R,2S,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)-2-methyl-5-methylenecyclopentyl]-1,9-dihydro-6H-purin-6-one;

(1S,2R,3R,5R)-5-(6-amino-9H-purin-9-yl)-3-(hydroxymethyl)-1-methyl-4-methylenecyclopentane-1,2-diol;

(1.alpha.OH,2.alpha.,3.beta., 5.beta.)-5-(6-amino-9H-purin-9-yl)-3-(hydroxymethyl)-1-methyl-4-methylenecyclopentane-1,2-diol;

(1RS,2R,3R,5R)-5-(4-amino-7H-pyrrolo[2,3-d]pyrimidin-7-yl)-3-(hydroxymethyl)-1-methylcyclopentanediol-1,2-diol;

(1S,2R,3R,5R)-5-(4-amino-7H pyrrolo[2,3-d]pyrimidin-7-yl)-3-(hydroxymethyl)-1-methylcyclopentanediol-1,2-diol;

(1RS,2R,3R,5R)-5-(6-amino-9H-purin-9-yl)-3-(hydroxymethyl)-1-methylcyclopentanediol-1,2-diol;

(1S,2R,3R,5R)-5-(6-amino-9H-purin-9-yl)-3-(hydroxymethyl)-1-methylcyclopentanediol-1,2-diol;

2-amino-9-[(1R,2RS,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)-2-methylcyclopentyl]-1,9-dihydro-6H-purin-6-one;

2-amino-9-[(1R,2S,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)-2-methylcyclopentyl]-1,9-dihydro-6H-purin-6-one;

2-amino-7-[(1R,2RS,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)-2-methylcyclopentyl]-3,7-dihydro-4H pyrrolo[2,3-d]pyrimidin-4-one; and 2-amino-7-[(1R,2S,3R,4R)-2,3-dihydroxy-4-(hydroxymethyl)-2-methylcyclopentyl]-3,7-dihydro-4H pyrrolo[2,3-d]pyrimidin-4-one;

and the corresponding 5'-triphosphates;
or a pharmaceutically acceptable salt thereof.

12. A pharmaceutical composition comprising a compound of Claim 1 and a pharmaceutically acceptable carrier.

13. A method of treating RNA-dependent RNA virus infection comprising administering to a mammal in need of such treatment a therapeutically effective amount of a compound according to Claim 1.

14. The method of Claim 13 wherein said RNA-dependent RNA
virus infection is a hepatitis C virus (HCV) infection.

15. The method of Claim 14 in combination with a therapeutically effective amount of another agent active against HCV.

16. The method of Claim 15 wherein said agent active against HCV is a 2'-C-Me-ribonucleoside; ribavirin; levovirin; thymosin alpha-1;
interferon-.beta.; an inhibitor of NS3 serine protease; an inhibitor of inosine monophosphate dehydrogenase; interferon-.alpha. or pegylated interferon-.alpha., alone or in combination with ribavirin or levovirin.

17. The method of Claim 16 wherein said agent active against HCV is interferon-.alpha. or pegylated interferon-.alpha., alone or in combination with ribavirin.

18. Use of a compound of Claim 1 for treatment of RNA-dependent RNA virus infection in a mammal.

19. The use of Claim 18 wherein said RNA-dependent RNA virus infection is HCV infection.

20. Use of a compound of Claim 1 in the manufacture of a medicament for treatment of RNA-dependent RNA virus infection in a mammal.

21. The use of Claim 20 wherein said RNA-dependent RNA virus infection is HCV infection.