CA2482560A1 - Centrifugal cytology system, chamber block and method for the preparation of treated monolayers of sample material - Google Patents
Centrifugal cytology system, chamber block and method for the preparation of treated monolayers of sample material Download PDFInfo
- Publication number
- CA2482560A1 CA2482560A1 CA002482560A CA2482560A CA2482560A1 CA 2482560 A1 CA2482560 A1 CA 2482560A1 CA 002482560 A CA002482560 A CA 002482560A CA 2482560 A CA2482560 A CA 2482560A CA 2482560 A1 CA2482560 A1 CA 2482560A1
- Authority
- CA
- Canada
- Prior art keywords
- sample material
- treating agent
- receiving surface
- surface member
- chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 title claims abstract 21
- 238000000034 method Methods 0.000 title claims abstract 12
- 239000003795 chemical substances by application Substances 0.000 claims abstract 20
- 239000002356 single layer Substances 0.000 claims abstract 9
- 239000012120 mounting media Substances 0.000 claims 3
- 238000005119 centrifugation Methods 0.000 claims 2
- 238000007789 sealing Methods 0.000 claims 2
- 239000012530 fluid Substances 0.000 claims 1
- 239000000463 material Substances 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000013049 sediment Substances 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0803—Disc shape
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00495—Centrifuges
- G01N2035/00504—Centrifuges combined with carousels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0439—Rotary sample carriers, i.e. carousels
- G01N2035/0446—Combinations of the above
- G01N2035/0449—Combinations of the above using centrifugal transport of liquid
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Centrifugal Separators (AREA)
Abstract
An apparatus and method for the automated preparation of treated monolayers of sample material, comprising: a centrifuge having a rotor (12) carrying removable chamber blocks (14); sample and reagent dispensers (26) and contro l systems (20). First, centrifugal force sediments sample material discretely to form a monolayer onto a receiving surface member (32) on one of the chamber blocks (14), while the same centrifugal force opens a valve (48) in the chamber block (14) to drain sample material. Then, centrifugal force deliver s sequentially into discrete chamber blocks (14) discrete treating agents, during which time the sampler material monolayer is held in place on the receiving surface member (32) by centrifugal force. Then, each chamber block (14) is drained centrifugally through its already opened valve (48). Each treated sampler material is confined to an individual chamber block (14). Batch and random access delivery of treating agents can be employed. Each chamber block (14) includes separate inlets for the sample and treating agen ts.
Claims (18)
1. A method for the automated preparation of at least one treated monolayer of sample material on a receiving surface member, comprising the steps of:
Fixing the orientation of said receiving surface member;
sedimenting, by centrifugation, the sample material onto said receiving surface member as a monolayer; and then centrifuging at least one treating agent downfield and directly onto the monolayer of sample material;
said steps of sedimenting and centrifuging being accomplished by the same centrifuge device, without removal of said receiving surface member.
Fixing the orientation of said receiving surface member;
sedimenting, by centrifugation, the sample material onto said receiving surface member as a monolayer; and then centrifuging at least one treating agent downfield and directly onto the monolayer of sample material;
said steps of sedimenting and centrifuging being accomplished by the same centrifuge device, without removal of said receiving surface member.
2. A method according to Claim 1, including the sequential steps of:
feeding the sample material vertically into one inlet of the centrifuge device; and supplying the treating agent horizontally into another inlet of the centrifuge device.
feeding the sample material vertically into one inlet of the centrifuge device; and supplying the treating agent horizontally into another inlet of the centrifuge device.
3. A method according to Claim 1 including the sequential steps of:
feeding the sample material into said centrifuge device prior to that device attaining sedimenting rotational speed;
opening a centrifugally responsive drain valve as the monolayer is being formed; and centrifugally draining treating agent from said centrifuge device after said monolayer is formed and treated.
feeding the sample material into said centrifuge device prior to that device attaining sedimenting rotational speed;
opening a centrifugally responsive drain valve as the monolayer is being formed; and centrifugally draining treating agent from said centrifuge device after said monolayer is formed and treated.
4. A method according to Claim 2 including the steps of:
establishing a plurality of discrete, similar and removable chamber blocks within a rotor portion of said centrifuge device; and defining said one and said another inlets to be proximate to each other in said chamber block.
establishing a plurality of discrete, similar and removable chamber blocks within a rotor portion of said centrifuge device; and defining said one and said another inlets to be proximate to each other in said chamber block.
5. A method according to Claim 4, including the step of:
supporting said receiving surface member on said chamber block, down field of both said one and said another inlets, for direct centrifugal receipt of both said sample material and treating agent.
supporting said receiving surface member on said chamber block, down field of both said one and said another inlets, for direct centrifugal receipt of both said sample material and treating agent.
6. A method according to Claim 1, including the step of:
controlling the concentration of said sedimenting, which forms said monolayer;
whereby, at least two different monolayers are formed from the same sample material simultaneously on said receiving surface member.
controlling the concentration of said sedimenting, which forms said monolayer;
whereby, at least two different monolayers are formed from the same sample material simultaneously on said receiving surface member.
7. A method according to Claim 6, in which said step of controlling is accomplished by:
establishing more than one sedimenting path length for the sample material to follow in reaching said receiving surface member.
establishing more than one sedimenting path length for the sample material to follow in reaching said receiving surface member.
8. A method according to Claim 1, in which said step of centrifuging comprises:
employing at least two sequential steps, each with a different treating agent.
employing at least two sequential steps, each with a different treating agent.
9. A method according to Claim 1, including the steps of:
supplying by centrifugal force to said receiving surface member a mounting medium;
flattening said mounting medium onto said receiving surface member; and curing said mounting medium upon said receiving surface member.
supplying by centrifugal force to said receiving surface member a mounting medium;
flattening said mounting medium onto said receiving surface member; and curing said mounting medium upon said receiving surface member.
10. A method according to Claim 5, in which said step of supporting includes:
sealing temporarily said receiving surface member onto said chamber block and, after said steps of sedimenting and centrifuging, removing said receiving surface member.
sealing temporarily said receiving surface member onto said chamber block and, after said steps of sedimenting and centrifuging, removing said receiving surface member.
11. A chamber block for use in a centrifugally operating system for preparing treated monolayers of sample material, said chamber block comprising:
a centrifugal up field side;
a centrifugal down field side;
a top and a bottom;
said top, proximate said up field side, provided with a vertically facing sample material input and a horizontally facing treating agent input;
said down field side adapted to receive in fluid sealing engagement a removable receiving surface member;
an output drain port positioned proximate said bottom and down field of said up field side; and a centrifugally operated valve for opening said drain port.
a centrifugal up field side;
a centrifugal down field side;
a top and a bottom;
said top, proximate said up field side, provided with a vertically facing sample material input and a horizontally facing treating agent input;
said down field side adapted to receive in fluid sealing engagement a removable receiving surface member;
an output drain port positioned proximate said bottom and down field of said up field side; and a centrifugally operated valve for opening said drain port.
12. A chamber block according to Claim 11, in which:
said valve is constructed and arranged to open during a time when sample material is being sedimented by centrifugation, as a monolayer, upon said receiving surface member.
said valve is constructed and arranged to open during a time when sample material is being sedimented by centrifugation, as a monolayer, upon said receiving surface member.
13. A chamber block according to Claim 12, in which:
said valve is a plug inserted into said drain port.
said valve is a plug inserted into said drain port.
14. A chamber block according to Claim 11, further including:
a volume reducing element positioned within said block and defining at least two paths of different lengths between said sample material input and said receiving surface member.
a volume reducing element positioned within said block and defining at least two paths of different lengths between said sample material input and said receiving surface member.
15. A chamber block according to Claim 11, including:
a treating agent holding trough, positioned up field of said treating agent input and in treating agent centrifugal flow connection therewith.
a treating agent holding trough, positioned up field of said treating agent input and in treating agent centrifugal flow connection therewith.
16. A plurality of chamber blocks according to Claim 11, adapted by be removably arrayed circumferentially around the exterior of a rotor of a centrifugally operable system;
said chamber blocks being mechanically and fluidically isolated from one another;
whereby sample material fed into said sample material input of one of said chamber blocks will not be cross-contaminated with sample material in a different chamber block; and treating agent fed into said treating agent input will not be cross-contaminated with treating agent in a different chamber block.
said chamber blocks being mechanically and fluidically isolated from one another;
whereby sample material fed into said sample material input of one of said chamber blocks will not be cross-contaminated with sample material in a different chamber block; and treating agent fed into said treating agent input will not be cross-contaminated with treating agent in a different chamber block.
17. A plurality of chamber blocks according to Claim 11, adapted to be removably arrayed circumferentially around the exterior of a rotor of a centrifugally operated system;
said rotor having a plurality of radially positioned treating agent troughs, isolated from each other and each radially alignable up field of one of said treating agent inputs, for centrifugal transfer of treating agent into a respective one of said chamber blocks.
said rotor having a plurality of radially positioned treating agent troughs, isolated from each other and each radially alignable up field of one of said treating agent inputs, for centrifugal transfer of treating agent into a respective one of said chamber blocks.
18. A plurality of circumferentially arrayed chamber blocks and a rotor, according to Claim 17, in which:
indexing means is further part of said to:
align a specific one of said chamber blocks and its specific one of said treating agent troughs with a treating agent dispenser, prior to the centrifugal transfer of treating agent.
indexing means is further part of said to:
align a specific one of said chamber blocks and its specific one of said treating agent troughs with a treating agent dispenser, prior to the centrifugal transfer of treating agent.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37254902P | 2002-04-13 | 2002-04-13 | |
| US60/372,549 | 2002-04-13 | ||
| PCT/US2003/011394 WO2003104801A1 (en) | 2002-04-13 | 2003-04-14 | Centrifugal cytology system, chamber block and method for the preparation of treated monolayers of sample material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2482560A1 true CA2482560A1 (en) | 2003-12-18 |
| CA2482560C CA2482560C (en) | 2013-12-03 |
Family
ID=29736056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2482560A Expired - Fee Related CA2482560C (en) | 2002-04-13 | 2003-04-14 | Centrifugal cytology system, chamber block and method for the preparation of treated monolayers of sample material |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8501096B2 (en) |
| EP (1) | EP1504260A4 (en) |
| AU (1) | AU2003234730A1 (en) |
| CA (1) | CA2482560C (en) |
| WO (1) | WO2003104801A1 (en) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7758816B2 (en) * | 2007-03-01 | 2010-07-20 | Wescor Inc. | Large area cytocentrifuge sample chamber |
| FR2931084B1 (en) * | 2008-05-19 | 2010-08-20 | Reactifs Ral | DEVICE FOR COLORING BLADES FOR MICROSCOPE EXAMINATION |
| WO2009152569A1 (en) | 2008-06-16 | 2009-12-23 | Leica Biosystems Melbourne Pty Ltd | Improvements in staining instruments and methods |
| EP2244083A1 (en) * | 2009-04-24 | 2010-10-27 | Koninklijke Philips Electronics N.V. | Method, apparatus, vessel and cytocentrifuge for liquid based cell preparation |
| US8877485B2 (en) * | 2009-12-09 | 2014-11-04 | Dako Denmark A/S | Apparatus and method for processing biological samples |
| DE102010003223B4 (en) * | 2010-03-24 | 2014-09-18 | Albert-Ludwigs-Universität Freiburg | Device for insertion into a rotor of a centrifuge, centrifuge and method for fluidic coupling of cavities |
| CN104535570A (en) * | 2014-12-24 | 2015-04-22 | 天津大学 | Device for testing single molecule force spectroscopy based on centrifugal force |
| DE102016213000A1 (en) * | 2016-07-15 | 2018-01-18 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Handling of liquids using a fluidic module having a plane of fluidity inclined with respect to a plane of rotation |
| NL2021969B1 (en) | 2018-10-05 | 2020-05-12 | Illumina Inc | Multi-valve fluid cartridge |
| FR3102847B1 (en) * | 2019-11-06 | 2021-10-15 | Ilsa | Cytocentrifugation devices |
| CN112170413B (en) * | 2020-10-09 | 2023-07-18 | 嘉兴晶铸生物科技有限公司 | Sedimentation bin cleaning device and sedimentation bin cleaning method |
| CN113109124B (en) * | 2021-04-09 | 2022-04-01 | 兴宏业（武汉）科技有限公司 | Negative pressure balance centrifugal flaking method |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
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| GB1417852A (en) | 1972-03-21 | 1975-12-17 | Coulter Electronics | Automatic chemical analysis apparatus |
| US3856470A (en) | 1973-01-10 | 1974-12-24 | Baxter Laboratories Inc | Rotor apparatus |
| US4119407A (en) | 1976-03-08 | 1978-10-10 | Bio-Dynamics, Inc. | Cuvette with reagent release means |
| NL7613690A (en) | 1976-12-09 | 1978-06-13 | Cenco Instr Bv | CYTOCENTRIFUGE. |
| US4234539A (en) | 1979-08-23 | 1980-11-18 | Coulter Electronics, Inc. | Apparatus for monitoring chemical reactions and employing moving photometer means |
| US4423699A (en) | 1979-02-28 | 1984-01-03 | E. I. Du Pont De Nemours & Co. | Centrifuge rotor apparatus for preparing particle spreads |
| US4250830A (en) | 1979-10-03 | 1981-02-17 | Leif Robert C | Swinging buckets |
| US4314523A (en) | 1980-03-19 | 1982-02-09 | E. I. Du Pont De Nemours And Company | Centrifuge rotor apparatus for preparing particle spreads |
| CA1152353A (en) | 1980-05-05 | 1983-08-23 | Georges Revillet | Multicuvette rotor for analyser |
| US4306514A (en) | 1980-08-05 | 1981-12-22 | E. I. Du Pont De Nemours And Company | Chamber block with a removable supernatant collection vial |
| US4327661A (en) | 1980-08-05 | 1982-05-04 | E. I. Du Pont De Nemours And Company | Chamber block having a supernatant collection receptacle therein |
| US4395493A (en) | 1981-05-14 | 1983-07-26 | Coulter Electronics, Inc. | Monolayer device using filter techniques |
| ATE34618T1 (en) * | 1982-09-02 | 1988-06-15 | Hettich Andreas Fa | CENTRIFUGATION CHAMBERS FOR THE CYTODIAGNOSTIC PREPARATION OF EPITHELIAL CELLS AND THEIR USE. |
| US4428323A (en) | 1982-11-22 | 1984-01-31 | E. I. Du Pont De Nemours And Company | Chamber block having a sample dam and a supernatant reentry barrier therein |
| US4576110A (en) * | 1983-05-19 | 1986-03-18 | E. I. Du Pont De Nemours And Company | Rotor having a chamber block with an absorbant plug |
| US5188935A (en) | 1984-05-31 | 1993-02-23 | Coulter Electronics, Inc. | Reagent system and method for identification, enumeration and examination of classes and subclasses of blood leukocytes |
| US4574729A (en) * | 1984-08-06 | 1986-03-11 | E. I. Du Pont De Nemours & Co. | Chamber block for a cytocentrifuge having centrifugal force responsive supernatant withdrawal means |
| GB8514590D0 (en) * | 1985-06-10 | 1985-07-10 | Shandon Southern Prod | Centrifugation |
| US4853188A (en) * | 1985-11-14 | 1989-08-01 | Kabushiki Kaisha Tiyoda Seisakusho | Cell for placing solid matters on a slide glass under centrifugal force |
| US4847205A (en) * | 1987-04-08 | 1989-07-11 | Martin Marietta Energy Systems, Inc. | Device and method for automated separation of a sample of whole blood into aliquots |
| US5180606A (en) | 1989-05-09 | 1993-01-19 | Wescor, Inc. | Apparatus for applying a controlled amount of reagent to a microscope slide or the like |
| US5266495A (en) | 1990-03-02 | 1993-11-30 | Cytyc Corporation | Method and apparatus for controlled instrumentation of particles with a filter device |
| US5143627A (en) | 1990-07-09 | 1992-09-01 | Cytyc Corporation | Method and apparatus for preparing cells for examination |
| US5282978A (en) | 1990-07-09 | 1994-02-01 | Cytyc Corporation | Specimen processor method and apparatus |
| US5364597A (en) | 1991-03-13 | 1994-11-15 | Cytyc Corporation | Apparatus for collection and transfer of particles |
| US5252228A (en) | 1991-11-05 | 1993-10-12 | Wescor, Inc. | Cytocentrifugation device, apparatus, and method |
| US5578452A (en) | 1992-01-16 | 1996-11-26 | Biogenex Laboratories | Enhancement of immunochemical staining in aldehyde-fixed tissues |
| US5346831A (en) | 1992-09-29 | 1994-09-13 | Hoffman-La Roche Inc. | Cytorich process system |
| US5419279A (en) | 1992-09-29 | 1995-05-30 | Hoffmann-La Roche Inc. | Apparatus for depositing and staining cytological material on a microscope slide |
| US5492837A (en) | 1993-08-27 | 1996-02-20 | Biogenex Laboratories | Mounting medium for microscope slide preparations |
| US5480484A (en) | 1994-03-01 | 1996-01-02 | Statspin Technologies | Cytology centrifuge apparatus |
| US5474687A (en) * | 1994-08-31 | 1995-12-12 | Activated Cell Therapy, Inc. | Methods for enriching CD34+ human hematopoietic progenitor cells |
| US5784193A (en) | 1996-06-28 | 1998-07-21 | Ferguson; Gary W. | Microscope slide with removable layer and method |
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| US5942129A (en) * | 1997-07-17 | 1999-08-24 | Shandon, Inc. | Cytology chamber with separate leg for fixative |
| US6008009A (en) * | 1998-04-17 | 1999-12-28 | Universities Space Research Association | Centrifuge-operated specimen staining method and apparatus |
-
2003
- 2003-04-14 WO PCT/US2003/011394 patent/WO2003104801A1/en not_active Ceased
- 2003-04-14 AU AU2003234730A patent/AU2003234730A1/en not_active Abandoned
- 2003-04-14 CA CA2482560A patent/CA2482560C/en not_active Expired - Fee Related
- 2003-04-14 US US10/512,337 patent/US8501096B2/en not_active Expired - Fee Related
- 2003-04-14 EP EP03728386.8A patent/EP1504260A4/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003234730A1 (en) | 2003-12-22 |
| WO2003104801A1 (en) | 2003-12-18 |
| EP1504260A1 (en) | 2005-02-09 |
| CA2482560C (en) | 2013-12-03 |
| US8501096B2 (en) | 2013-08-06 |
| EP1504260A4 (en) | 2014-04-02 |
| US20050260100A1 (en) | 2005-11-24 |
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| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20170418 |