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CA2339420A1 - Evolution genique specifique du domaine - Google Patents

Evolution genique specifique du domaine Download PDF

Info

Publication number
CA2339420A1
CA2339420A1 CA002339420A CA2339420A CA2339420A1 CA 2339420 A1 CA2339420 A1 CA 2339420A1 CA 002339420 A CA002339420 A CA 002339420A CA 2339420 A CA2339420 A CA 2339420A CA 2339420 A1 CA2339420 A1 CA 2339420A1
Authority
CA
Canada
Prior art keywords
nucleic acid
sequence
target nucleic
library
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002339420A
Other languages
English (en)
Inventor
David Zarling
Gurucharan Reddy
Sushma Pati
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tapestry Pharmaceuticals Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/133,934 external-priority patent/US6074853A/en
Application filed by Individual filed Critical Individual
Publication of CA2339420A1 publication Critical patent/CA2339420A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

On décrit des compositions et des procédés de mise au point rapide de domaines protéiques spécifiques au moyen d'une recombinase et d'intermédiaires de recombinaison.
CA002339420A 1998-08-12 1999-08-12 Evolution genique specifique du domaine Abandoned CA2339420A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US9633098P 1998-08-12 1998-08-12
US60/096,330 1998-08-14
US09/133,934 US6074853A (en) 1997-03-21 1998-08-14 Sequence alterations using homologous recombination
US09/133,934 1998-08-14
PCT/US1999/018393 WO2000009755A2 (fr) 1998-08-12 1999-08-12 Evolution genique specifique du domaine

Publications (1)

Publication Number Publication Date
CA2339420A1 true CA2339420A1 (fr) 2000-02-24

Family

ID=26791582

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002339420A Abandoned CA2339420A1 (fr) 1998-08-12 1999-08-12 Evolution genique specifique du domaine

Country Status (5)

Country Link
EP (1) EP1105472A2 (fr)
JP (1) JP2002522096A (fr)
AU (1) AU772879B2 (fr)
CA (1) CA2339420A1 (fr)
WO (1) WO2000009755A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2401677A1 (fr) * 2000-03-03 2001-09-13 University Of Utah Research Foundation Procede de ciblage genique
EP1152058A1 (fr) * 2000-05-03 2001-11-07 Institut Curie Méthodes et compositions permettant d'effectuer la recombinaison homologue
CA2405520A1 (fr) * 2000-05-23 2001-11-29 California Institute Of Technology Recombinaison de genes et mise au point de proteines hybrides
AU2001266978A1 (en) * 2000-06-14 2001-12-24 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
JP4616988B2 (ja) * 2000-12-20 2011-01-19 株式会社アイシン・コスモス研究所 三本鎖ｄｎａ形成技術を応用したｄｎａ多型検出方法
WO2003087341A2 (fr) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Mutagenese chromosomique ciblee au moyen de nucleases en doigt a zinc
GB0218803D0 (en) * 2002-08-13 2002-09-18 Nl Kanker I Targeted gene modification by single-standed DNA oligonucleotides
JP2006502748A (ja) 2002-09-05 2006-01-26 カリフォルニア インスティテュート オブ テクノロジー 遺伝子ターゲッティングを誘発するキメラヌクレアーゼの使用方法
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US11136609B2 (en) * 2015-11-09 2021-10-05 National University Corporation Kyoto Institute Of Technology Separating agent for human serum-derived IgG polyclonal antibodies, and method for separating human serum-derived IgG polyclonal antibodies using same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022443A1 (fr) * 1992-04-24 1993-11-11 Sri International Ciblage de sequences homologues in vivo dans des cellules eukaryotiques
US5731411A (en) * 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
JPH08140685A (ja) * 1994-11-22 1996-06-04 Takara Shuzo Co Ltd 部位特異的変異導入方法
WO1998033890A1 (fr) * 1997-02-03 1998-08-06 Hepatix Inc. Serfine-1 (rasp-1) associee a la regeneration humaine et methodes d'utilisation
US5948653A (en) * 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination

Also Published As

Publication number Publication date
AU5774899A (en) 2000-03-06
WO2000009755A9 (fr) 2000-08-03
WO2000009755A3 (fr) 2000-06-08
WO2000009755A2 (fr) 2000-02-24
JP2002522096A (ja) 2002-07-23
AU772879B2 (en) 2004-05-13
EP1105472A2 (fr) 2001-06-13

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US6074853A (en) Sequence alterations using homologous recombination
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WO1998042727A9 (fr) Modifications de sequence par recombinaison homologue
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US20020061530A1 (en) Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation
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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued