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CA2333481A1 - Vecteurs lentiviraux - Google Patents

Vecteurs lentiviraux Download PDF

Info

Publication number
CA2333481A1
CA2333481A1 CA002333481A CA2333481A CA2333481A1 CA 2333481 A1 CA2333481 A1 CA 2333481A1 CA 002333481 A CA002333481 A CA 002333481A CA 2333481 A CA2333481 A CA 2333481A CA 2333481 A1 CA2333481 A1 CA 2333481A1
Authority
CA
Canada
Prior art keywords
vector
packaging
gene
cell
hiv
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002333481A
Other languages
English (en)
Inventor
Lung-Ji Chang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from PCT/US1999/011516 external-priority patent/WO2000000600A2/fr
Publication of CA2333481A1 publication Critical patent/CA2333481A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne un vecteur d'encapsidation qui comprend une séquence nucléotidique codant pour les protéines Gag et Pol d'un lentivirus de référence, ledit vecteur différant dudit lentivirus au moins par les caractéristiques suivantes : a) soit son site donneur d'épissage majeur est délété, soit il est présent, mais sa séquence, bien qu'il soit fonctionnel, diffère suffisamment de celle dudit lentivirus pour que ledit site donneur ne soit pas un site potentiel de recombinaison homologue entre ledit virus d'encapsidation et ledit vecteur de référence ; et b) il lui manque un signal d'encapsidation majeur fonctionnel. Ce vecteur, une fois introduit dans une cellule hôte appropriée, est capable de faire en sorte que ladite cellule, soit par expression à partir dudit vecteur seul, soit par coexpression à partir dudit vecteur et d'un second vecteur assurant l'expression d'une protéine d'enveloppe compatible, produise des particules de vecteur d'encapsidation renfermant des protéines Gag et Pol fonctionnelles et possédant une enveloppe normale ou pseudotypée, lesdites particules étant dépourvues de la forme ARN du vecteur d'encapsidation à l'issue de l'étape (b) ci-dessus. Ladite cellule, à l'issue de ladite expression ou coexpression, produit des particules qui encapsulent la forme ARN d'un vecteur de transduction possédant un signal d'encapsidation compatible et fonctionnel quand on introduit dans ladite cellule ledit vecteur de transduction.
CA002333481A 1998-05-26 1999-05-26 Vecteurs lentiviraux Abandoned CA2333481A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8663598P 1998-05-26 1998-05-26
US60/086,635 1998-05-26
PCT/US1999/011516 WO2000000600A2 (fr) 1997-09-22 1999-05-26 Vecteurs lentiviraux

Publications (1)

Publication Number Publication Date
CA2333481A1 true CA2333481A1 (fr) 2000-01-06

Family

ID=22199866

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002333481A Abandoned CA2333481A1 (fr) 1998-05-26 1999-05-26 Vecteurs lentiviraux

Country Status (4)

Country Link
EP (1) EP1082447A2 (fr)
AU (2) AU773015B2 (fr)
CA (1) CA2333481A1 (fr)
WO (1) WO1999061598A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6790657B1 (en) 1999-01-07 2004-09-14 The United States Of America As Represented By The Department Of Health And Human Services Lentivirus vector system
AU2495300A (en) * 1999-01-07 2000-07-24 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Lentivirus vector system
WO2001083730A2 (fr) * 2000-04-28 2001-11-08 The Trustees Of The University Of Pennsylvania Vecteurs lentiviraux de recombinaison pseudotypes dans des enveloppes contenant des domaines de liaison de filovirus
EP1468094A4 (fr) * 2001-12-24 2005-01-26 Es Cell Int Pte Ltd Procede de transduction de cellules es
US7399278B1 (en) 2003-05-05 2008-07-15 Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center Method and system for measuring amniotic fluid volume and/or assessing fetal weight
EP4103723A1 (fr) * 2020-02-13 2022-12-21 Oxford BioMedica (UK) Limited Production de vecteurs lentiviraux

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1645635A3 (fr) * 1989-08-18 2010-07-07 Oxford Biomedica (UK) Limited Retrovirus recombinants défectifs pour la réplication exprimant un palliatif
US5436146A (en) * 1989-09-07 1995-07-25 The Trustees Of Princeton University Helper-free stocks of recombinant adeno-associated virus vectors
WO1992007573A1 (fr) * 1990-10-31 1992-05-14 Somatix Therapy Corporation Modification genetique de cellules endotheliales
US6051427A (en) * 1993-06-11 2000-04-18 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
US6200811B1 (en) * 1996-04-02 2001-03-13 The Regents Of The University Of California Cell transformation vector comprising an HIV-2 packaging site nucleic acid and an HIV-1 GAG protein

Also Published As

Publication number Publication date
AU4312699A (en) 2000-01-17
WO1999061598A2 (fr) 1999-12-02
WO1999061598A3 (fr) 2000-04-13
AU4207899A (en) 1999-12-13
EP1082447A2 (fr) 2001-03-14
AU773015B2 (en) 2004-05-13

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued