CA2329975A1 - Procede d'obtention de cellules souches - Google Patents
Procede d'obtention de cellules souches Download PDFInfo
- Publication number
- CA2329975A1 CA2329975A1 CA002329975A CA2329975A CA2329975A1 CA 2329975 A1 CA2329975 A1 CA 2329975A1 CA 002329975 A CA002329975 A CA 002329975A CA 2329975 A CA2329975 A CA 2329975A CA 2329975 A1 CA2329975 A1 CA 2329975A1
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- Prior art keywords
- cell
- cells
- marker
- nucleus
- oocyte
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2517/00—Cells related to new breeds of animals
- C12N2517/04—Cells produced using nuclear transfer
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Developmental Biology & Embryology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un procédé permettant d'obtenir une cellule souche. Ce procédé consiste à obtenir une cellule somatique contenant un gène codant pour un marqueur qui est exprimé de manière différentielle dans (a) les cellules souches requises et (b) les cellules autres que les cellules souches requises. Ce procédé consiste ensuite à obtenir un ovocyte énucléé, à transférer le noyau de la cellule somatique dans l'ovocyte énucléé pour former un ovocyte transgénique puis à cultiver ce dernier ovocyte pour produire une culture de cellules dérivées de l'ovocyte transgénique. Ce procédé consiste enfin à identifier une cellule de la culture exprimant le marqueur, ce qui indique alors que le noyau de cette cellule a été reprogrammé. L'invention traite également de dosages pour des facteurs qui peuvent reprogrammer les noyaux et les cellules souches obtenus directement ou indirectement à l'aide des procédés de reprogrammation nucléaires selon l'invention
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9809178.8 | 1998-04-29 | ||
| GBGB9809178.8A GB9809178D0 (en) | 1998-04-29 | 1998-04-29 | Nuclear reprogramming of somatic cells |
| PCT/GB1999/001333 WO1999055841A2 (fr) | 1998-04-29 | 1999-04-29 | Procede d'obtention de cellules souches |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2329975A1 true CA2329975A1 (fr) | 1999-11-04 |
Family
ID=10831203
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002329975A Abandoned CA2329975A1 (fr) | 1998-04-29 | 1999-04-29 | Procede d'obtention de cellules souches |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP1073719A2 (fr) |
| JP (1) | JP2002512787A (fr) |
| AU (1) | AU3621299A (fr) |
| CA (1) | CA2329975A1 (fr) |
| GB (1) | GB9809178D0 (fr) |
| IL (1) | IL139322A0 (fr) |
| MX (1) | MXPA00010473A (fr) |
| WO (1) | WO1999055841A2 (fr) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6667176B1 (en) | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
| EP1196426A4 (fr) * | 1999-06-30 | 2003-09-03 | Advanced Cell Tech Inc | Transfert cytoplasmique permettant de reduire la differentiation de cellules receveuses |
| WO2001018193A1 (fr) * | 1999-09-07 | 2001-03-15 | Advanced Cell Technology, Inc. | Procede destine a produire des cellules et des tissus immunocompatibles par techniques de transfert nucleaire |
| WO2001018236A1 (fr) * | 1999-09-07 | 2001-03-15 | Advanced Cell Technology, Inc. | Methodes de reparation des sequences d'adn a repetition en tandem et d'extension de la duree de vie cellulaire par transfert nucleaire |
| CN1425064A (zh) * | 1999-12-20 | 2003-06-18 | 马萨诸塞大学 | 通过交叉物种核移植制备的胚胎或干样细胞 |
| US20020168763A1 (en) * | 2000-11-30 | 2002-11-14 | Yan Wen Liang | Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same |
| US20050130144A1 (en) * | 2001-09-21 | 2005-06-16 | Norio Nakatsuji | Method of screening reprogramming factor, reprogramming factor screened by the method, method of using the reprogramming factor, method of differentiating undifferentiated fused cells and method of constructing cell, tissues and organs |
| HUP0401692A3 (en) * | 2001-11-06 | 2005-02-28 | Sheng Huizhen | Preparing somatic embryo by utilizing rabbit oocyte |
| CN1683524A (zh) * | 2003-04-08 | 2005-10-19 | 东南大学 | 用体细胞核移植技术制备移核胚胎干细胞的方法 |
| US7682828B2 (en) | 2003-11-26 | 2010-03-23 | Whitehead Institute For Biomedical Research | Methods for reprogramming somatic cells |
| US7964401B2 (en) | 2004-02-19 | 2011-06-21 | Kyoto University | Screening method for somatic cell nuclear reprogramming substance affecting ECAT2 and ECAT3 |
| US20090227032A1 (en) | 2005-12-13 | 2009-09-10 | Kyoto University | Nuclear reprogramming factor and induced pluripotent stem cells |
| US8278104B2 (en) | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
| CN105861443A (zh) * | 2007-04-07 | 2016-08-17 | 怀特黑德生物医学研究所 | 体细胞重编程 |
| JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| ES2722198T3 (es) | 2008-05-02 | 2019-08-08 | Univ Kyoto | Método de reprogramación nuclear |
| CN105671065A (zh) | 2008-06-13 | 2016-06-15 | 怀特黑德生物医学研究所 | 细胞编程和重编程 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9308271D0 (en) * | 1993-04-21 | 1993-06-02 | Univ Edinburgh | Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method |
| GB9401380D0 (en) * | 1994-01-25 | 1994-03-23 | Pharmaceutical Proteins Ltd | Embryonic cell isolation |
| GB9517780D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| US6482937B1 (en) * | 1997-10-09 | 2002-11-19 | Biotransplant, Inc. | Porcine Oct-4 promoter |
-
1998
- 1998-04-29 GB GBGB9809178.8A patent/GB9809178D0/en not_active Ceased
-
1999
- 1999-04-29 WO PCT/GB1999/001333 patent/WO1999055841A2/fr not_active Ceased
- 1999-04-29 EP EP99918186A patent/EP1073719A2/fr not_active Withdrawn
- 1999-04-29 JP JP2000545985A patent/JP2002512787A/ja active Pending
- 1999-04-29 CA CA002329975A patent/CA2329975A1/fr not_active Abandoned
- 1999-04-29 MX MXPA00010473A patent/MXPA00010473A/es unknown
- 1999-04-29 AU AU36212/99A patent/AU3621299A/en not_active Abandoned
- 1999-04-29 IL IL13932299A patent/IL139322A0/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| MXPA00010473A (es) | 2002-04-24 |
| WO1999055841A3 (fr) | 2000-01-06 |
| GB9809178D0 (en) | 1998-07-01 |
| EP1073719A2 (fr) | 2001-02-07 |
| AU3621299A (en) | 1999-11-16 |
| JP2002512787A (ja) | 2002-05-08 |
| WO1999055841A2 (fr) | 1999-11-04 |
| IL139322A0 (en) | 2001-11-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |