CA2325562A1 - Complexes de proteine lyst et proteines a interaction lyst - Google Patents

Info

Publication number

CA2325562A1

CA2325562A1 CA002325562A CA2325562A CA2325562A1 CA 2325562 A1 CA2325562 A1 CA 2325562A1 CA 002325562 A CA002325562 A CA 002325562A CA 2325562 A CA2325562 A CA 2325562A CA 2325562 A1 CA2325562 A1 CA 2325562A1

Authority

CA

Canada

Prior art keywords

lyst

protein

complex

seq

derivative

Prior art date

1998-04-03

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Abandoned

Links

• Espacenet
• Global Dossier
• CIPO
• Discuss

• 102100034956 Poly(rC)-binding protein 4 Human genes 0.000 title claims abstract description 135
• 101000735365 Homo sapiens Poly(rC)-binding protein 4 Proteins 0.000 title claims abstract description 132
• 102000007474 Multiprotein Complexes Human genes 0.000 title description 3
• 108010085220 Multiprotein Complexes Proteins 0.000 title description 3
• 108090000623 proteins and genes Proteins 0.000 claims abstract description 407
• 101001018064 Homo sapiens Lysosomal-trafficking regulator Proteins 0.000 claims abstract description 358
• 102100033472 Lysosomal-trafficking regulator Human genes 0.000 claims abstract description 354
• 102000004169 proteins and genes Human genes 0.000 claims abstract description 313
• 235000018102 proteins Nutrition 0.000 claims abstract description 310
• 102100034114 DnaJ homolog subfamily C member 14 Human genes 0.000 claims abstract description 132
• 102100026302 Zinc finger protein 521 Human genes 0.000 claims abstract description 128
• 101000870166 Homo sapiens DnaJ homolog subfamily C member 14 Proteins 0.000 claims abstract description 127
• 101150098307 LIP3 gene Proteins 0.000 claims abstract description 127
• 102100027556 39S ribosomal protein L17, mitochondrial Human genes 0.000 claims abstract description 124
• 101100489464 Homo sapiens ZNF521 gene Proteins 0.000 claims abstract description 124
• 101100074851 Candida albicans (strain SC5314 / ATCC MYA-2876) LIP7 gene Proteins 0.000 claims abstract description 122
• 101100074853 Candida albicans (strain SC5314 / ATCC MYA-2876) LIP9 gene Proteins 0.000 claims abstract description 121
• 101000924474 Homo sapiens Annexin A2 Proteins 0.000 claims abstract description 121
• 101100009574 Oryza sativa subsp. japonica DHN1 gene Proteins 0.000 claims abstract description 121
• 239000012634 fragment Substances 0.000 claims abstract description 116
• YAFQFNOUYXZVPZ-UHFFFAOYSA-N liproxstatin-1 Chemical compound ClC1=CC=CC(CNC=2C3(CCNCC3)NC3=CC=CC=C3N=2)=C1 YAFQFNOUYXZVPZ-UHFFFAOYSA-N 0.000 claims abstract description 114
• 238000000034 method Methods 0.000 claims abstract description 109
• 102100023441 Centromere protein J Human genes 0.000 claims abstract description 96
• 101100343350 Candida albicans (strain SC5314 / ATCC MYA-2876) LIP10 gene Proteins 0.000 claims abstract description 95
• 101000907924 Homo sapiens Centromere protein J Proteins 0.000 claims abstract description 94
• 101000693082 Homo sapiens Serine/threonine-protein kinase 11-interacting protein Proteins 0.000 claims abstract description 93
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 81
• -1 Hrs Proteins 0.000 claims abstract description 72
• 102000004899 14-3-3 Proteins Human genes 0.000 claims abstract description 67
• 101710112812 14-3-3 protein Proteins 0.000 claims abstract description 55
• 102000000584 Calmodulin Human genes 0.000 claims abstract description 48
• 108010041952 Calmodulin Proteins 0.000 claims abstract description 48
• 102000012012 beta Karyopherins Human genes 0.000 claims abstract description 48
• 108010075890 beta Karyopherins Proteins 0.000 claims abstract description 48
• 108010032028 norbin Proteins 0.000 claims abstract description 47
• 102000052052 Casein Kinase II Human genes 0.000 claims abstract description 46
• 108010010919 Casein Kinase II Proteins 0.000 claims abstract description 46
• SLPJGDQJLTYWCI-UHFFFAOYSA-N dimethyl-(4,5,6,7-tetrabromo-1h-benzoimidazol-2-yl)-amine Chemical compound BrC1=C(Br)C(Br)=C2NC(N(C)C)=NC2=C1Br SLPJGDQJLTYWCI-UHFFFAOYSA-N 0.000 claims abstract description 46
• 102100033098 Neurochondrin Human genes 0.000 claims abstract description 45
• 101000679249 Homo sapiens 40S ribosomal protein S3a Proteins 0.000 claims abstract description 44
• 101001130763 Homo sapiens Protein OS-9 Proteins 0.000 claims abstract description 44
• 102100021070 Mediator of RNA polymerase II transcription subunit 12 Human genes 0.000 claims abstract description 44
• 101710179284 Mediator of RNA polymerase II transcription subunit 12 Proteins 0.000 claims abstract description 44
• 101100279264 Mus musculus Efs gene Proteins 0.000 claims abstract description 44
• 108010065729 Troponin I Proteins 0.000 claims abstract description 44
• 102000013394 Troponin I Human genes 0.000 claims abstract description 44
• 108010075803 neurochondrin Proteins 0.000 claims abstract description 44
• 102100022600 40S ribosomal protein S3a Human genes 0.000 claims abstract description 43
• 101000839071 Rattus norvegicus Heterogeneous nuclear ribonucleoprotein M Proteins 0.000 claims abstract description 43
• 102100024419 28S ribosomal protein S31, mitochondrial Human genes 0.000 claims abstract description 42
• 101710119973 28S ribosomal protein S31, mitochondrial Proteins 0.000 claims abstract description 42
• 101000723543 Homo sapiens 14-3-3 protein theta Proteins 0.000 claims abstract description 41
• 102100031492 Protein OS-9 Human genes 0.000 claims abstract description 40
• 201000010099 disease Diseases 0.000 claims abstract description 40
• 108090000806 Atrophin-1 Proteins 0.000 claims abstract description 39
• 102100040493 Centrobin Human genes 0.000 claims abstract description 39
• 101000926103 Homo sapiens Rab GDP dissociation inhibitor alpha Proteins 0.000 claims abstract description 39
• 101000749889 Homo sapiens Centrobin Proteins 0.000 claims abstract description 38
• 102100034335 Rab GDP dissociation inhibitor alpha Human genes 0.000 claims abstract description 38
• 101000950687 Homo sapiens Mitogen-activated protein kinase 7 Proteins 0.000 claims abstract description 36
• 102100037805 Mitogen-activated protein kinase 7 Human genes 0.000 claims abstract description 36
• 102100027831 14-3-3 protein theta Human genes 0.000 claims abstract description 35
• 101000827417 Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) 30S ribosomal protein S3 Proteins 0.000 claims abstract description 35
• 101000841231 Homo sapiens Elongation factor 1-alpha 2 Proteins 0.000 claims abstract description 35
• 101001009091 Homo sapiens Hematopoietic lineage cell-specific protein Proteins 0.000 claims abstract description 35
• 238000012216 screening Methods 0.000 claims abstract description 27
• 206010073391 Platelet dysfunction Diseases 0.000 claims abstract description 15
• 208000012657 Atopic disease Diseases 0.000 claims abstract description 12
• 208000023275 Autoimmune disease Diseases 0.000 claims abstract description 12
• 208000015122 neurodegenerative disease Diseases 0.000 claims abstract description 12
• 230000004770 neurodegeneration Effects 0.000 claims abstract description 11
• 206010028980 Neoplasm Diseases 0.000 claims abstract description 8
• 208000012641 Pigmentation disease Diseases 0.000 claims abstract description 8
• 201000011510 cancer Diseases 0.000 claims abstract description 4
• 101000649979 Homo sapiens Vacuolar protein sorting-associated protein VTA1 homolog Proteins 0.000 claims abstract 36
• 102100028298 Vacuolar protein sorting-associated protein VTA1 homolog Human genes 0.000 claims abstract 36
• 101000662028 Homo sapiens Ubiquitin-associated domain-containing protein 1 Proteins 0.000 claims abstract 30
• 102100037937 Ubiquitin-associated domain-containing protein 1 Human genes 0.000 claims abstract 30
• 101100289192 Pseudomonas fragi lips gene Proteins 0.000 claims description 98
• 150000007523 nucleic acids Chemical class 0.000 claims description 96
• 102100037279 Secretoglobin family 1D member 2 Human genes 0.000 claims description 91
• 101000739195 Homo sapiens Secretoglobin family 1D member 2 Proteins 0.000 claims description 84
• 102000039446 nucleic acids Human genes 0.000 claims description 84
• 108020004707 nucleic acids Proteins 0.000 claims description 84
• 210000004027 cell Anatomy 0.000 claims description 73
• 125000003729 nucleotide group Chemical group 0.000 claims description 69
• 239000002773 nucleotide Substances 0.000 claims description 67
• 150000001413 amino acids Chemical class 0.000 claims description 54
• 108020004414 DNA Proteins 0.000 claims description 42
• 208000035475 disorder Diseases 0.000 claims description 41
• 230000027455 binding Effects 0.000 claims description 40
• 241000282414 Homo sapiens Species 0.000 claims description 39
• 102000004321 Atrophin-1 Human genes 0.000 claims description 37
• 125000003275 alpha amino acid group Chemical group 0.000 claims description 36
• 230000000694 effects Effects 0.000 claims description 36
• 108091028043 Nucleic acid sequence Proteins 0.000 claims description 31
• 230000000295 complement effect Effects 0.000 claims description 29
• 239000000523 sample Substances 0.000 claims description 27
• 239000013598 vector Substances 0.000 claims description 27
• 241001465754 Metazoa Species 0.000 claims description 24
• 101710102461 Lipase member I Proteins 0.000 claims description 22
• 102100030659 Lipase member I Human genes 0.000 claims description 22
• 238000011282 treatment Methods 0.000 claims description 22
• 230000001594 aberrant effect Effects 0.000 claims description 19
• 230000015572 biosynthetic process Effects 0.000 claims description 19
• 230000005714 functional activity Effects 0.000 claims description 19
• 238000000338 in vitro Methods 0.000 claims description 16
• 239000008194 pharmaceutical composition Substances 0.000 claims description 16
• 108020001507 fusion proteins Proteins 0.000 claims description 14
• 102000037865 fusion proteins Human genes 0.000 claims description 14
• 238000012360 testing method Methods 0.000 claims description 13
• 108091026890 Coding region Proteins 0.000 claims description 11
• 208000036142 Viral infection Diseases 0.000 claims description 9
• 239000000126 substance Substances 0.000 claims description 9
• 230000009385 viral infection Effects 0.000 claims description 9
• 230000008859 change Effects 0.000 claims description 8
• 230000004071 biological effect Effects 0.000 claims description 7
• 238000006243 chemical reaction Methods 0.000 claims description 7
• 208000024891 symptom Diseases 0.000 claims description 7
• 230000000692 anti-sense effect Effects 0.000 claims description 5
• 208000031169 hemorrhagic disease Diseases 0.000 claims description 5
• 230000003463 hyperproliferative effect Effects 0.000 claims description 5
• 206010062713 Haemorrhagic diathesis Diseases 0.000 claims description 4
• 230000007423 decrease Effects 0.000 claims description 4
• 230000003247 decreasing effect Effects 0.000 claims description 4
• 230000001965 increasing effect Effects 0.000 claims description 4
• 230000009467 reduction Effects 0.000 claims description 4
• 108020004711 Nucleic Acid Probes Proteins 0.000 claims description 3
• 239000002853 nucleic acid probe Substances 0.000 claims description 3
• 230000019612 pigmentation Effects 0.000 claims description 3
• 230000037452 priming Effects 0.000 claims description 3
• 230000003321 amplification Effects 0.000 claims description 2
• 238000003199 nucleic acid amplification method Methods 0.000 claims description 2
• 239000003937 drug carrier Substances 0.000 claims 13
• 230000002265 prevention Effects 0.000 claims 12
• 101001088179 Homo sapiens DNA-directed RNA polymerases I, II, and III subunit RPABC1 Proteins 0.000 claims 4
• 101001081220 Homo sapiens RanBP-type and C3HC4-type zinc finger-containing protein 1 Proteins 0.000 claims 4
• 102100027716 RanBP-type and C3HC4-type zinc finger-containing protein 1 Human genes 0.000 claims 4
• 210000004748 cultured cell Anatomy 0.000 claims 4
• 108090000144 Human Proteins Proteins 0.000 claims 3
• 102000003839 Human Proteins Human genes 0.000 claims 3
• 230000001093 anti-cancer Effects 0.000 claims 3
• 238000002744 homologous recombination Methods 0.000 claims 3
• 230000006801 homologous recombination Effects 0.000 claims 3
• 230000035755 proliferation Effects 0.000 claims 3
• 230000004083 survival effect Effects 0.000 claims 3
• 230000004614 tumor growth Effects 0.000 claims 3
• 108700019146 Transgenes Proteins 0.000 claims 2
• 239000003795 chemical substances by application Substances 0.000 claims 2
• 230000000259 anti-tumor effect Effects 0.000 claims 1
• 238000002743 insertional mutagenesis Methods 0.000 claims 1
• 230000003211 malignant effect Effects 0.000 claims 1
• 238000012544 monitoring process Methods 0.000 claims 1
• 210000004881 tumor cell Anatomy 0.000 claims 1
• 231100000588 tumorigenic Toxicity 0.000 claims 1
• 230000000381 tumorigenic effect Effects 0.000 claims 1
• 108700026220 vif Genes Proteins 0.000 claims 1
• 208000037765 diseases and disorders Diseases 0.000 abstract description 3
• 230000003612 virological effect Effects 0.000 abstract description 3
• 238000003158 yeast two-hybrid assay Methods 0.000 abstract description 2
• 102100020741 Atrophin-1 Human genes 0.000 abstract 2
• 241001502050 Acis Species 0.000 abstract 1
• 229940024606 amino acid Drugs 0.000 description 55
• 235000001014 amino acid Nutrition 0.000 description 54
• 238000003556 assay Methods 0.000 description 28
• 102100029938 Serine/threonine-protein kinase SMG1 Human genes 0.000 description 27
• 230000014509 gene expression Effects 0.000 description 26
• 230000003993 interaction Effects 0.000 description 22
• 208000031879 Chédiak-Higashi syndrome Diseases 0.000 description 21
• 210000004556 brain Anatomy 0.000 description 21
• 238000009396 hybridization Methods 0.000 description 18
• 239000000047 product Substances 0.000 description 18
• 208000000398 DiGeorge Syndrome Diseases 0.000 description 15
• 239000002299 complementary DNA Substances 0.000 description 15
• 239000013604 expression vector Substances 0.000 description 15
• 230000028023 exocytosis Effects 0.000 description 14
• 230000006870 function Effects 0.000 description 13
• 238000004519 manufacturing process Methods 0.000 description 12
• 230000019491 signal transduction Effects 0.000 description 12
• 230000014616 translation Effects 0.000 description 12
• 230000001605 fetal effect Effects 0.000 description 11
• 108020004999 messenger RNA Proteins 0.000 description 11
• 230000001105 regulatory effect Effects 0.000 description 11
• 239000000243 solution Substances 0.000 description 11
• 230000032258 transport Effects 0.000 description 11
• 108020004705 Codon Proteins 0.000 description 10
• 241000894007 species Species 0.000 description 10
• 101001020310 Homo sapiens Liprin-alpha-1 Proteins 0.000 description 9
• 102100035684 Liprin-alpha-1 Human genes 0.000 description 9
• 108090000315 Protein Kinase C Proteins 0.000 description 9
• 102000003923 Protein Kinase C Human genes 0.000 description 9
• 230000002378 acidificating effect Effects 0.000 description 9
• 210000003712 lysosome Anatomy 0.000 description 9
• 230000001868 lysosomic effect Effects 0.000 description 9
• 239000000203 mixture Substances 0.000 description 9
• 238000013519 translation Methods 0.000 description 9
• 108700026244 Open Reading Frames Proteins 0.000 description 8
• 108020004511 Recombinant DNA Proteins 0.000 description 8
• 241000700605 Viruses Species 0.000 description 8
• 239000002253 acid Substances 0.000 description 8
• 102000053583 human ERD5 Human genes 0.000 description 8
• 108700013139 human ERD5 Proteins 0.000 description 8
• 230000002132 lysosomal effect Effects 0.000 description 8
• 210000001519 tissue Anatomy 0.000 description 8
• 230000028973 vesicle-mediated transport Effects 0.000 description 8
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 7
• 101150082107 LIP10 gene Proteins 0.000 description 7
• 101150115381 LIP9 gene Proteins 0.000 description 7
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 7
• 238000004458 analytical method Methods 0.000 description 7
• 239000011575 calcium Substances 0.000 description 7
• 229910052791 calcium Inorganic materials 0.000 description 7
• 239000013599 cloning vector Substances 0.000 description 7
• 101150031897 lipB gene Proteins 0.000 description 7
• 230000001404 mediated effect Effects 0.000 description 7
• 230000004048 modification Effects 0.000 description 7
• 238000012986 modification Methods 0.000 description 7
• 238000003752 polymerase chain reaction Methods 0.000 description 7
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 7
• 230000002441 reversible effect Effects 0.000 description 7
• 230000011664 signaling Effects 0.000 description 7
• 238000006467 substitution reaction Methods 0.000 description 7
• FUOOLUPWFVMBKG-UHFFFAOYSA-N 2-Aminoisobutyric acid Chemical compound CC(C)(N)C(O)=O FUOOLUPWFVMBKG-UHFFFAOYSA-N 0.000 description 6
• 108090000412 Protein-Tyrosine Kinases Proteins 0.000 description 6
• 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
• 238000013459 approach Methods 0.000 description 6
• 210000004899 c-terminal region Anatomy 0.000 description 6
• 230000007812 deficiency Effects 0.000 description 6
• 102000015694 estrogen receptors Human genes 0.000 description 6
• 108010038795 estrogen receptors Proteins 0.000 description 6
• 150000002632 lipids Chemical class 0.000 description 6
• 230000035790 physiological processes and functions Effects 0.000 description 6
• 239000013612 plasmid Substances 0.000 description 6
• 108090000765 processed proteins & peptides Proteins 0.000 description 6
• 108091008146 restriction endonucleases Proteins 0.000 description 6
• 230000001225 therapeutic effect Effects 0.000 description 6
• 108700020469 14-3-3 Proteins 0.000 description 5
• 208000010543 22q11.2 deletion syndrome Diseases 0.000 description 5
• 241000972773 Aulopiformes Species 0.000 description 5
• 241000282412 Homo Species 0.000 description 5
• 208000003367 Hypopigmentation Diseases 0.000 description 5
• 101150032410 LIP6 gene Proteins 0.000 description 5
• 101150094893 LIP7 gene Proteins 0.000 description 5
• 241000699670 Mus sp. Species 0.000 description 5
• 208000037273 Pathologic Processes Diseases 0.000 description 5
• 102000004022 Protein-Tyrosine Kinases Human genes 0.000 description 5
• 102000000583 SNARE Proteins Human genes 0.000 description 5
• 108010041948 SNARE Proteins Proteins 0.000 description 5
• 150000007513 acids Chemical class 0.000 description 5
• 125000000539 amino acid group Chemical group 0.000 description 5
• 210000001772 blood platelet Anatomy 0.000 description 5
• 231100000504 carcinogenesis Toxicity 0.000 description 5
• 238000010367 cloning Methods 0.000 description 5
• 230000009918 complex formation Effects 0.000 description 5
• 239000008187 granular material Substances 0.000 description 5
• 210000004408 hybridoma Anatomy 0.000 description 5
• 230000002209 hydrophobic effect Effects 0.000 description 5
• 230000003425 hypopigmentation Effects 0.000 description 5
• 238000003018 immunoassay Methods 0.000 description 5
• 230000005847 immunogenicity Effects 0.000 description 5
• 238000003780 insertion Methods 0.000 description 5
• 230000037431 insertion Effects 0.000 description 5
• 239000003550 marker Substances 0.000 description 5
• 238000010369 molecular cloning Methods 0.000 description 5
• 230000009054 pathological process Effects 0.000 description 5
• 238000012545 processing Methods 0.000 description 5
• 235000019515 salmon Nutrition 0.000 description 5
• 239000000758 substrate Substances 0.000 description 5
• 238000001086 yeast two-hybrid system Methods 0.000 description 5
• 208000005623 Carcinogenesis Diseases 0.000 description 4
• 201000008163 Dentatorubral pallidoluysian atrophy Diseases 0.000 description 4
• 238000002965 ELISA Methods 0.000 description 4
• 241000196324 Embryophyta Species 0.000 description 4
• 229920001917 Ficoll Polymers 0.000 description 4
• 241000238631 Hexapoda Species 0.000 description 4
• 101000648012 Homo sapiens Signal transducing adapter molecule 1 Proteins 0.000 description 4
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 4
• 241000699666 Mus <mouse, genus> Species 0.000 description 4
• 206010061309 Neoplasm progression Diseases 0.000 description 4
• 208000012902 Nervous system disease Diseases 0.000 description 4
• 102100025245 Signal transducing adapter molecule 1 Human genes 0.000 description 4
• 102000004183 Synaptosomal-Associated Protein 25 Human genes 0.000 description 4
• 108010057722 Synaptosomal-Associated Protein 25 Proteins 0.000 description 4
• 230000021736 acetylation Effects 0.000 description 4
• 238000006640 acetylation reaction Methods 0.000 description 4
• 238000007792 addition Methods 0.000 description 4
• 102000009899 alpha Karyopherins Human genes 0.000 description 4
• 108010077099 alpha Karyopherins Proteins 0.000 description 4
• 230000036952 cancer formation Effects 0.000 description 4
• 230000003915 cell function Effects 0.000 description 4
• 239000003153 chemical reaction reagent Substances 0.000 description 4
• XVOYSCVBGLVSOL-UHFFFAOYSA-N cysteic acid Chemical class OC(=O)C(N)CS(O)(=O)=O XVOYSCVBGLVSOL-UHFFFAOYSA-N 0.000 description 4
• 210000000805 cytoplasm Anatomy 0.000 description 4
• 230000007547 defect Effects 0.000 description 4
• 230000003412 degenerative effect Effects 0.000 description 4
• 230000001419 dependent effect Effects 0.000 description 4
• 230000004927 fusion Effects 0.000 description 4
• 230000013595 glycosylation Effects 0.000 description 4
• 238000006206 glycosylation reaction Methods 0.000 description 4
• 102000049826 human LYST Human genes 0.000 description 4
• 239000003112 inhibitor Substances 0.000 description 4
• 210000005061 intracellular organelle Anatomy 0.000 description 4
• 125000005647 linker group Chemical group 0.000 description 4
• 101150018767 lips gene Proteins 0.000 description 4
• 210000004185 liver Anatomy 0.000 description 4
• 210000004379 membrane Anatomy 0.000 description 4
• 239000012528 membrane Substances 0.000 description 4
• 230000034217 membrane fusion Effects 0.000 description 4
• 229930182817 methionine Natural products 0.000 description 4
• 238000003032 molecular docking Methods 0.000 description 4
• 238000007899 nucleic acid hybridization Methods 0.000 description 4
• 210000004940 nucleus Anatomy 0.000 description 4
• 201000007909 oculocutaneous albinism Diseases 0.000 description 4
• 230000026731 phosphorylation Effects 0.000 description 4
• 238000006366 phosphorylation reaction Methods 0.000 description 4
• 238000003259 recombinant expression Methods 0.000 description 4
• FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 4
• 238000012163 sequencing technique Methods 0.000 description 4
• 238000010561 standard procedure Methods 0.000 description 4
• 238000003786 synthesis reaction Methods 0.000 description 4
• 238000013518 transcription Methods 0.000 description 4
• 230000035897 transcription Effects 0.000 description 4
• 230000009466 transformation Effects 0.000 description 4
• 230000005751 tumor progression Effects 0.000 description 4
• 241000701447 unidentified baculovirus Species 0.000 description 4
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
• 206010001557 Albinism Diseases 0.000 description 3
• 108091033380 Coding strand Proteins 0.000 description 3
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
• 101100132878 Homo sapiens NCDN gene Proteins 0.000 description 3
• 241000713772 Human immunodeficiency virus 1 Species 0.000 description 3
• 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 3
• 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 3
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 3
• 101150041552 LIP4 gene Proteins 0.000 description 3
• 101001018066 Mus musculus Lysosomal-trafficking regulator Proteins 0.000 description 3
• 108091034117 Oligonucleotide Proteins 0.000 description 3
• 108091000080 Phosphotransferase Proteins 0.000 description 3
• 239000004365 Protease Substances 0.000 description 3
• 241000700159 Rattus Species 0.000 description 3
• 241000700618 Vaccinia virus Species 0.000 description 3
• 230000005856 abnormality Effects 0.000 description 3
• 208000006673 asthma Diseases 0.000 description 3
• 238000000376 autoradiography Methods 0.000 description 3
• 230000033228 biological regulation Effects 0.000 description 3
• 230000001413 cellular effect Effects 0.000 description 3
• 150000001875 compounds Chemical class 0.000 description 3
• 230000001086 cytosolic effect Effects 0.000 description 3
• 238000012217 deletion Methods 0.000 description 3
• 230000037430 deletion Effects 0.000 description 3
• 238000003745 diagnosis Methods 0.000 description 3
• 239000003623 enhancer Substances 0.000 description 3
• 230000012010 growth Effects 0.000 description 3
• 210000003630 histaminocyte Anatomy 0.000 description 3
• 230000008676 import Effects 0.000 description 3
• 238000001727 in vivo Methods 0.000 description 3
• 230000000977 initiatory effect Effects 0.000 description 3
• 230000003834 intracellular effect Effects 0.000 description 3
• 230000031146 intracellular signal transduction Effects 0.000 description 3
• 238000002955 isolation Methods 0.000 description 3
• 210000002510 keratinocyte Anatomy 0.000 description 3
• 210000000265 leukocyte Anatomy 0.000 description 3
• 101150077696 lip-1 gene Proteins 0.000 description 3
• 230000007246 mechanism Effects 0.000 description 3
• 210000002752 melanocyte Anatomy 0.000 description 3
• 230000007935 neutral effect Effects 0.000 description 3
• 210000003463 organelle Anatomy 0.000 description 3
• 102000020233 phosphotransferase Human genes 0.000 description 3
• 230000001323 posttranslational effect Effects 0.000 description 3
• 239000013615 primer Substances 0.000 description 3
• 230000008569 process Effects 0.000 description 3
• 238000004393 prognosis Methods 0.000 description 3
• 102000005969 steroid hormone receptors Human genes 0.000 description 3
• 108020003113 steroid hormone receptors Proteins 0.000 description 3
• 238000012546 transfer Methods 0.000 description 3
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 3
• 241000701161 unidentified adenovirus Species 0.000 description 3
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 2
• BVAUMRCGVHUWOZ-ZETCQYMHSA-N (2s)-2-(cyclohexylazaniumyl)propanoate Chemical class OC(=O)[C@H](C)NC1CCCCC1 BVAUMRCGVHUWOZ-ZETCQYMHSA-N 0.000 description 2
• MRTPISKDZDHEQI-YFKPBYRVSA-N (2s)-2-(tert-butylamino)propanoic acid Chemical class OC(=O)[C@H](C)NC(C)(C)C MRTPISKDZDHEQI-YFKPBYRVSA-N 0.000 description 2
• NPDBDJFLKKQMCM-SCSAIBSYSA-N (2s)-2-amino-3,3-dimethylbutanoic acid Chemical class CC(C)(C)[C@H](N)C(O)=O NPDBDJFLKKQMCM-SCSAIBSYSA-N 0.000 description 2
• 101150028074 2 gene Proteins 0.000 description 2
• 108091006112 ATPases Proteins 0.000 description 2
• 102000057290 Adenosine Triphosphatases Human genes 0.000 description 2
• 241001516864 Allende Species 0.000 description 2
• 239000004475 Arginine Substances 0.000 description 2
• 241000271566 Aves Species 0.000 description 2
• 241000894006 Bacteria Species 0.000 description 2
• 241000283690 Bos taurus Species 0.000 description 2
• 208000031229 Cardiomyopathies Diseases 0.000 description 2
• 208000012239 Developmental disease Diseases 0.000 description 2
• 102100024125 Embryonal Fyn-associated substrate Human genes 0.000 description 2
• 102100030013 Endoribonuclease Human genes 0.000 description 2
• 101710199605 Endoribonuclease Proteins 0.000 description 2
• 102000004190 Enzymes Human genes 0.000 description 2
• 108090000790 Enzymes Proteins 0.000 description 2
• 241000701959 Escherichia virus Lambda Species 0.000 description 2
• ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
• 241000233866 Fungi Species 0.000 description 2
• 241000287828 Gallus gallus Species 0.000 description 2
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
• 208000031886 HIV Infections Diseases 0.000 description 2
• 102100022557 Hepatocyte growth factor-regulated tyrosine kinase substrate Human genes 0.000 description 2
• PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 2
• 108090001061 Insulin Proteins 0.000 description 2
• 102000011781 Karyopherins Human genes 0.000 description 2
• 108010062228 Karyopherins Proteins 0.000 description 2
• SNDPXSYFESPGGJ-BYPYZUCNSA-N L-2-aminopentanoic acid Chemical compound CCC[C@H](N)C(O)=O SNDPXSYFESPGGJ-BYPYZUCNSA-N 0.000 description 2
• AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 2
• ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
• ZGUNAGUHMKGQNY-ZETCQYMHSA-N L-alpha-phenylglycine zwitterion Chemical class OC(=O)[C@@H](N)C1=CC=CC=C1 ZGUNAGUHMKGQNY-ZETCQYMHSA-N 0.000 description 2
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
• RHGKLRLOHDJJDR-BYPYZUCNSA-N L-citrulline Chemical class NC(=O)NCCC[C@H]([NH3+])C([O-])=O RHGKLRLOHDJJDR-BYPYZUCNSA-N 0.000 description 2
• SNDPXSYFESPGGJ-UHFFFAOYSA-N L-norVal-OH Natural products CCCC(N)C(O)=O SNDPXSYFESPGGJ-UHFFFAOYSA-N 0.000 description 2
• LRQKBLKVPFOOQJ-YFKPBYRVSA-N L-norleucine Chemical compound CCCC[C@H]([NH3+])C([O-])=O LRQKBLKVPFOOQJ-YFKPBYRVSA-N 0.000 description 2
• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
• 101150021155 LIP2 gene Proteins 0.000 description 2
• 101800000135 N-terminal protein Proteins 0.000 description 2
• RHGKLRLOHDJJDR-UHFFFAOYSA-N Ndelta-carbamoyl-DL-ornithine Natural products OC(=O)C(N)CCCNC(N)=O RHGKLRLOHDJJDR-UHFFFAOYSA-N 0.000 description 2
• 208000025966 Neurological disease Diseases 0.000 description 2
• 108010077850 Nuclear Localization Signals Proteins 0.000 description 2
• 102000007999 Nuclear Proteins Human genes 0.000 description 2
• 108010089610 Nuclear Proteins Proteins 0.000 description 2
• 108700020796 Oncogene Proteins 0.000 description 2
• AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 2
• UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 2
• 101800001452 P1 proteinase Proteins 0.000 description 2
• 108090000526 Papain Proteins 0.000 description 2
• 102000007982 Phosphoproteins Human genes 0.000 description 2
• 108010089430 Phosphoproteins Proteins 0.000 description 2
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 2
• 102000001253 Protein Kinase Human genes 0.000 description 2
• 101710150344 Protein Rev Proteins 0.000 description 2
• 108010077895 Sarcosine Proteins 0.000 description 2
• 101710113029 Serine/threonine-protein kinase Proteins 0.000 description 2
• 108010019040 Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Proteins 0.000 description 2
• 102000006384 Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Human genes 0.000 description 2
• 102000040945 Transcription factor Human genes 0.000 description 2
• 108091023040 Transcription factor Proteins 0.000 description 2
• 102000004903 Troponin Human genes 0.000 description 2
• 108090001027 Troponin Proteins 0.000 description 2
• 108091000117 Tyrosine 3-Monooxygenase Proteins 0.000 description 2
• 102000048218 Tyrosine 3-monooxygenases Human genes 0.000 description 2
• 208000024780 Urticaria Diseases 0.000 description 2
• 241000251539 Vertebrata <Metazoa> Species 0.000 description 2
• 101100166799 Xenopus laevis tnrc4-a gene Proteins 0.000 description 2
• HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
• 230000009471 action Effects 0.000 description 2
• 239000002671 adjuvant Substances 0.000 description 2
• 239000000556 agonist Substances 0.000 description 2
• QWCKQJZIFLGMSD-UHFFFAOYSA-N alpha-aminobutyric acid Chemical compound CCC(N)C(O)=O QWCKQJZIFLGMSD-UHFFFAOYSA-N 0.000 description 2
• 239000005557 antagonist Substances 0.000 description 2
• 239000003242 anti bacterial agent Substances 0.000 description 2
• 230000006907 apoptotic process Effects 0.000 description 2
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
• 210000003719 b-lymphocyte Anatomy 0.000 description 2
• UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 description 2
• 230000003197 catalytic effect Effects 0.000 description 2
• 230000024245 cell differentiation Effects 0.000 description 2
• 230000010261 cell growth Effects 0.000 description 2
• 230000002490 cerebral effect Effects 0.000 description 2
• 235000013330 chicken meat Nutrition 0.000 description 2
• 210000000349 chromosome Anatomy 0.000 description 2
• 229960002173 citrulline Drugs 0.000 description 2
• 235000013477 citrulline Nutrition 0.000 description 2
• 238000004440 column chromatography Methods 0.000 description 2
• 239000013601 cosmid vector Substances 0.000 description 2
• 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 2
• 230000002950 deficient Effects 0.000 description 2
• 238000011161 development Methods 0.000 description 2
• 230000018109 developmental process Effects 0.000 description 2
• 230000029087 digestion Effects 0.000 description 2
• PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 2
• 239000003814 drug Substances 0.000 description 2
• 239000012636 effector Substances 0.000 description 2
• 229940088598 enzyme Drugs 0.000 description 2
• 229940011871 estrogen Drugs 0.000 description 2
• 239000000262 estrogen Substances 0.000 description 2
• 230000022244 formylation Effects 0.000 description 2
• 238000006170 formylation reaction Methods 0.000 description 2
• BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 2
• 239000000499 gel Substances 0.000 description 2
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
• 230000001456 gonadotroph Effects 0.000 description 2
• 108010023829 hepatocyte growth factor-regulated tyrosine kinase substrate Proteins 0.000 description 2
• 229960002591 hydroxyproline Drugs 0.000 description 2
• 230000001900 immune effect Effects 0.000 description 2
• 230000001771 impaired effect Effects 0.000 description 2
• 230000001976 improved effect Effects 0.000 description 2
• 239000000411 inducer Substances 0.000 description 2
• 208000015181 infectious disease Diseases 0.000 description 2
• 230000002401 inhibitory effect Effects 0.000 description 2
• 239000003999 initiator Substances 0.000 description 2
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
• 239000003446 ligand Substances 0.000 description 2
• 210000004698 lymphocyte Anatomy 0.000 description 2
• 210000004962 mammalian cell Anatomy 0.000 description 2
• 210000003593 megakaryocyte Anatomy 0.000 description 2
• 210000002780 melanosome Anatomy 0.000 description 2
• 125000000250 methylamino group Chemical group [H]N(*)C([H])([H])[H] 0.000 description 2
• 238000002703 mutagenesis Methods 0.000 description 2
• 231100000350 mutagenesis Toxicity 0.000 description 2
• 230000035772 mutation Effects 0.000 description 2
• 210000000822 natural killer cell Anatomy 0.000 description 2
• 229930014626 natural product Natural products 0.000 description 2
• 230000000626 neurodegenerative effect Effects 0.000 description 2
• 230000014511 neuron projection development Effects 0.000 description 2
• 238000011275 oncology therapy Methods 0.000 description 2
• 229960003104 ornithine Drugs 0.000 description 2
• 230000003647 oxidation Effects 0.000 description 2
• 238000007254 oxidation reaction Methods 0.000 description 2
• 229940055729 papain Drugs 0.000 description 2
• 235000019834 papain Nutrition 0.000 description 2
• 230000037361 pathway Effects 0.000 description 2
• 239000002243 precursor Substances 0.000 description 2
• 230000013823 prenylation Effects 0.000 description 2
• 102000004196 processed proteins & peptides Human genes 0.000 description 2
• 108060006633 protein kinase Proteins 0.000 description 2
• 230000007398 protein translocation Effects 0.000 description 2
• 230000004850 protein–protein interaction Effects 0.000 description 2
• 238000000746 purification Methods 0.000 description 2
• 230000006798 recombination Effects 0.000 description 2
• 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
• 230000010076 replication Effects 0.000 description 2
• 230000004044 response Effects 0.000 description 2
• 229940043230 sarcosine Drugs 0.000 description 2
• 210000002966 serum Anatomy 0.000 description 2
• 238000012916 structural analysis Methods 0.000 description 2
• 210000002182 synaptic membrane Anatomy 0.000 description 2
• 230000002194 synthesizing effect Effects 0.000 description 2
• 210000001541 thymus gland Anatomy 0.000 description 2
• FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 2
• 230000002103 transcriptional effect Effects 0.000 description 2
• 238000010396 two-hybrid screening Methods 0.000 description 2
• 241001515965 unidentified phage Species 0.000 description 2
• 230000011215 vesicle docking Effects 0.000 description 2
• 238000005406 washing Methods 0.000 description 2
• 239000011701 zinc Substances 0.000 description 2
• 229910052725 zinc Inorganic materials 0.000 description 2
• ASWBNKHCZGQVJV-UHFFFAOYSA-N (3-hexadecanoyloxy-2-hydroxypropyl) 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(O)COP([O-])(=O)OCC[N+](C)(C)C ASWBNKHCZGQVJV-UHFFFAOYSA-N 0.000 description 1
• UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 description 1
• QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
• HNZZAEBYPPNVOF-UHFFFAOYSA-N 2-amino-3-methylbutanoic acid;2-amino-3-methylpentanoic acid Chemical compound CC(C)C(N)C(O)=O.CCC(C)C(N)C(O)=O HNZZAEBYPPNVOF-UHFFFAOYSA-N 0.000 description 1
• SLXKOJJOQWFEFD-UHFFFAOYSA-N 6-aminohexanoic acid Chemical compound NCCCCCC(O)=O SLXKOJJOQWFEFD-UHFFFAOYSA-N 0.000 description 1
• 102100023990 60S ribosomal protein L17 Human genes 0.000 description 1
• 208000030507 AIDS Diseases 0.000 description 1
• 101800000263 Acidic protein Proteins 0.000 description 1
• 108010085238 Actins Proteins 0.000 description 1
• 102000007469 Actins Human genes 0.000 description 1
• 229920000936 Agarose Polymers 0.000 description 1
• 108010088751 Albumins Proteins 0.000 description 1
• 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
• 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
• 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
• 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
• 208000035285 Allergic Seasonal Rhinitis Diseases 0.000 description 1
• 208000009115 Anorectal Malformations Diseases 0.000 description 1
• 206010002961 Aplasia Diseases 0.000 description 1
• 101001010152 Aplysia californica Probable glutathione transferase Proteins 0.000 description 1
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
• 206010003591 Ataxia Diseases 0.000 description 1
• 210000002237 B-cell of pancreatic islet Anatomy 0.000 description 1
• 101100048435 Caenorhabditis elegans unc-18 gene Proteins 0.000 description 1
• 102000005701 Calcium-Binding Proteins Human genes 0.000 description 1
• 108010045403 Calcium-Binding Proteins Proteins 0.000 description 1
• 101710116137 Calcium/calmodulin-dependent protein kinase II Proteins 0.000 description 1
• 241000282465 Canis Species 0.000 description 1
• 241000282472 Canis lupus familiaris Species 0.000 description 1
• 101710167800 Capsid assembly scaffolding protein Proteins 0.000 description 1
• 101710132601 Capsid protein Proteins 0.000 description 1
• 102000014914 Carrier Proteins Human genes 0.000 description 1
• 102000005403 Casein Kinases Human genes 0.000 description 1
• 108010031425 Casein Kinases Proteins 0.000 description 1
• 241000701489 Cauliflower mosaic virus Species 0.000 description 1
• 206010057248 Cell death Diseases 0.000 description 1
• 101710142763 Centrobin Proteins 0.000 description 1
• 241000282693 Cercopithecidae Species 0.000 description 1
• 206010008025 Cerebellar ataxia Diseases 0.000 description 1
• 206010008754 Choreoathetosis Diseases 0.000 description 1
• 108090000317 Chymotrypsin Proteins 0.000 description 1
• 102000005853 Clathrin Human genes 0.000 description 1
• 108010019874 Clathrin Proteins 0.000 description 1
• 241000186216 Corynebacterium Species 0.000 description 1
• 108010024986 Cyclin-Dependent Kinase 2 Proteins 0.000 description 1
• 108010025464 Cyclin-Dependent Kinase 4 Proteins 0.000 description 1
• 102100036239 Cyclin-dependent kinase 2 Human genes 0.000 description 1
• 102100036252 Cyclin-dependent kinase 4 Human genes 0.000 description 1
• 102000004127 Cytokines Human genes 0.000 description 1
• 108090000695 Cytokines Proteins 0.000 description 1
• 102000010831 Cytoskeletal Proteins Human genes 0.000 description 1
• 108010037414 Cytoskeletal Proteins Proteins 0.000 description 1
• 102000053602 DNA Human genes 0.000 description 1
• 239000003155 DNA primer Substances 0.000 description 1
• 206010012289 Dementia Diseases 0.000 description 1
• 108010053770 Deoxyribonucleases Proteins 0.000 description 1
• 102000016911 Deoxyribonucleases Human genes 0.000 description 1
• 101710156582 Embryonal Fyn-associated substrate Proteins 0.000 description 1
• 241000283073 Equus caballus Species 0.000 description 1
• 241000490229 Eucephalus Species 0.000 description 1
• 102000042493 Eukaryotic family Human genes 0.000 description 1
• 108091078334 Eukaryotic family Proteins 0.000 description 1
• 108010007457 Extracellular Signal-Regulated MAP Kinases Proteins 0.000 description 1
• 102000007665 Extracellular Signal-Regulated MAP Kinases Human genes 0.000 description 1
• 241000282324 Felis Species 0.000 description 1
• 108090000852 Forkhead Transcription Factors Proteins 0.000 description 1
• 102000001534 GDP dissociation inhibitor Human genes 0.000 description 1
• 102000018898 GTPase-Activating Proteins Human genes 0.000 description 1
• 108091006094 GTPase-accelerating proteins Proteins 0.000 description 1
• 108010001515 Galectin 4 Proteins 0.000 description 1
• 102100039556 Galectin-4 Human genes 0.000 description 1
• 102000042092 Glucose transporter family Human genes 0.000 description 1
• 108091052347 Glucose transporter family Proteins 0.000 description 1
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
• 102000005720 Glutathione transferase Human genes 0.000 description 1
• 108010070675 Glutathione transferase Proteins 0.000 description 1
• 239000004471 Glycine Substances 0.000 description 1
• 108010092964 Guanine Nucleotide Dissociation Inhibitors Proteins 0.000 description 1
• 108010067218 Guanine Nucleotide Exchange Factors Proteins 0.000 description 1
• 102000016285 Guanine Nucleotide Exchange Factors Human genes 0.000 description 1
• 102100021519 Hemoglobin subunit beta Human genes 0.000 description 1
• 108091005904 Hemoglobin subunit beta Proteins 0.000 description 1
• 101000818893 Homo sapiens 14-3-3 protein beta/alpha Proteins 0.000 description 1
• 101000689847 Homo sapiens 28S ribosomal protein S31, mitochondrial Proteins 0.000 description 1
• 101000650291 Homo sapiens 39S ribosomal protein L17, mitochondrial Proteins 0.000 description 1
• 101000882584 Homo sapiens Estrogen receptor Proteins 0.000 description 1
• 101000735358 Homo sapiens Poly(rC)-binding protein 2 Proteins 0.000 description 1
• 101000813777 Homo sapiens Splicing factor ESS-2 homolog Proteins 0.000 description 1
• 101001050288 Homo sapiens Transcription factor Jun Proteins 0.000 description 1
• 101000785690 Homo sapiens Zinc finger protein 521 Proteins 0.000 description 1
• 208000013038 Hypocalcemia Diseases 0.000 description 1
• XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
• 206010061598 Immunodeficiency Diseases 0.000 description 1
• 208000029462 Immunodeficiency disease Diseases 0.000 description 1
• 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
• 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
• 108700005091 Immunoglobulin Genes Proteins 0.000 description 1
• 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 1
• 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
• 208000022559 Inflammatory bowel disease Diseases 0.000 description 1
• 102000004877 Insulin Human genes 0.000 description 1
• 108010034219 Insulin Receptor Substrate Proteins Proteins 0.000 description 1
• 102000009433 Insulin Receptor Substrate Proteins Human genes 0.000 description 1
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
• HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
• KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
• 101150055724 LYST gene Proteins 0.000 description 1
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
• 102000003960 Ligases Human genes 0.000 description 1
• 108090000364 Ligases Proteins 0.000 description 1
• 208000005777 Lupus Nephritis Diseases 0.000 description 1
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
• 239000004472 Lysine Substances 0.000 description 1
• 102000043136 MAP kinase family Human genes 0.000 description 1
• 108091054455 MAP kinase family Proteins 0.000 description 1
• 241000124008 Mammalia Species 0.000 description 1
• PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
• 241000184321 Mazus Species 0.000 description 1
• 108090000157 Metallothionein Proteins 0.000 description 1
• 108700027650 Mitogen-Activated Protein Kinase 7 Proteins 0.000 description 1
• 102000046796 Mitogen-Activated Protein Kinase 7 Human genes 0.000 description 1
• 241000713333 Mouse mammary tumor virus Species 0.000 description 1
• 101100236342 Mus musculus Lyst gene Proteins 0.000 description 1
• 208000029578 Muscle disease Diseases 0.000 description 1
• 208000021642 Muscular disease Diseases 0.000 description 1
• 101710107068 Myelin basic protein Proteins 0.000 description 1
• 208000036572 Myoclonic epilepsy Diseases 0.000 description 1
• 208000000592 Nasal Polyps Diseases 0.000 description 1
• 208000008457 Neurologic Manifestations Diseases 0.000 description 1
• 108010066154 Nuclear Export Signals Proteins 0.000 description 1
• 108091005461 Nucleic proteins Proteins 0.000 description 1
• 241000283973 Oryctolagus cuniculus Species 0.000 description 1
• 238000012408 PCR amplification Methods 0.000 description 1
• 108010067372 Pancreatic elastase Proteins 0.000 description 1
• 102000057297 Pepsin A Human genes 0.000 description 1
• 108090000284 Pepsin A Proteins 0.000 description 1
• 108091005804 Peptidases Proteins 0.000 description 1
• 208000013544 Platelet disease Diseases 0.000 description 1
• 241000276498 Pollachius virens Species 0.000 description 1
• 102100034961 Poly(rC)-binding protein 2 Human genes 0.000 description 1
• 241000288906 Primates Species 0.000 description 1
• 101710130420 Probable capsid assembly scaffolding protein Proteins 0.000 description 1
• 208000033063 Progressive myoclonic epilepsy Diseases 0.000 description 1
• 101710151715 Protein 7 Proteins 0.000 description 1
• 108010029485 Protein Isoforms Proteins 0.000 description 1
• 102000001708 Protein Isoforms Human genes 0.000 description 1
• 102000052575 Proto-Oncogene Human genes 0.000 description 1
• 108700020978 Proto-Oncogene Proteins 0.000 description 1
• 102000007568 Proto-Oncogene Proteins c-fos Human genes 0.000 description 1
• 108010071563 Proto-Oncogene Proteins c-fos Proteins 0.000 description 1
• 102000001788 Proto-Oncogene Proteins c-raf Human genes 0.000 description 1
• 108010029869 Proto-Oncogene Proteins c-raf Proteins 0.000 description 1
• 108010010469 Qa-SNARE Proteins Proteins 0.000 description 1
• 108010005730 R-SNARE Proteins Proteins 0.000 description 1
• 102100033479 RAF proto-oncogene serine/threonine-protein kinase Human genes 0.000 description 1
• 101710141955 RAF proto-oncogene serine/threonine-protein kinase Proteins 0.000 description 1
• 101100177642 Rattus norvegicus Hgs gene Proteins 0.000 description 1
• 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
• 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
• 201000000582 Retinoblastoma Diseases 0.000 description 1
• 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
• 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
• 241000714474 Rous sarcoma virus Species 0.000 description 1
• 206010039491 Sarcoma Diseases 0.000 description 1
• 101710204410 Scaffold protein Proteins 0.000 description 1
• 238000012300 Sequence Analysis Methods 0.000 description 1
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
• 108091081024 Start codon Proteins 0.000 description 1
• 102000005465 Stathmin Human genes 0.000 description 1
• 108050003387 Stathmin Proteins 0.000 description 1
• 102000002215 Synaptobrevin Human genes 0.000 description 1
• 102000050389 Syntaxin Human genes 0.000 description 1
• 230000006044 T cell activation Effects 0.000 description 1
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
• 239000004473 Threonine Substances 0.000 description 1
• 102000006601 Thymidine Kinase Human genes 0.000 description 1
• 108020004440 Thymidine kinase Proteins 0.000 description 1
• 102100023132 Transcription factor Jun Human genes 0.000 description 1
• 108090000631 Trypsin Proteins 0.000 description 1
• 102000004142 Trypsin Human genes 0.000 description 1
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
• 102000005506 Tryptophan Hydroxylase Human genes 0.000 description 1
• 108010031944 Tryptophan Hydroxylase Proteins 0.000 description 1
• YJQCOFNZVFGCAF-UHFFFAOYSA-N Tunicamycin II Natural products O1C(CC(O)C2C(C(O)C(O2)N2C(NC(=O)C=C2)=O)O)C(O)C(O)C(NC(=O)C=CCCCCCCCCC(C)C)C1OC1OC(CO)C(O)C(O)C1NC(C)=O YJQCOFNZVFGCAF-UHFFFAOYSA-N 0.000 description 1
• 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 1
• 102000003425 Tyrosinase Human genes 0.000 description 1
• 108060008724 Tyrosinase Proteins 0.000 description 1
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
• 230000035508 accumulation Effects 0.000 description 1
• 238000009825 accumulation Methods 0.000 description 1
• 239000012190 activator Substances 0.000 description 1
• 239000013543 active substance Substances 0.000 description 1
• 230000001919 adrenal effect Effects 0.000 description 1
• 238000000246 agarose gel electrophoresis Methods 0.000 description 1
• 235000004279 alanine Nutrition 0.000 description 1
• 108010050122 alpha 1-Antitrypsin Proteins 0.000 description 1
• 108010026331 alpha-Fetoproteins Proteins 0.000 description 1
• 230000004075 alteration Effects 0.000 description 1
• WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
• 230000009435 amidation Effects 0.000 description 1
• 238000007112 amidation reaction Methods 0.000 description 1
• 229960002684 aminocaproic acid Drugs 0.000 description 1
• 238000010171 animal model Methods 0.000 description 1
• 229940088710 antibiotic agent Drugs 0.000 description 1
• 230000000890 antigenic effect Effects 0.000 description 1
• 238000003782 apoptosis assay Methods 0.000 description 1
• 235000009582 asparagine Nutrition 0.000 description 1
• 229960001230 asparagine Drugs 0.000 description 1
• 235000003704 aspartic acid Nutrition 0.000 description 1
• 230000001363 autoimmune Effects 0.000 description 1
• 230000006470 autoimmune attack Effects 0.000 description 1
• 230000001580 bacterial effect Effects 0.000 description 1
• 239000013602 bacteriophage vector Substances 0.000 description 1
• 230000006399 behavior Effects 0.000 description 1
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
• 108091008324 binding proteins Proteins 0.000 description 1
• 230000003115 biocidal effect Effects 0.000 description 1
• 230000005540 biological transmission Effects 0.000 description 1
• 230000000903 blocking effect Effects 0.000 description 1
• 210000004369 blood Anatomy 0.000 description 1
• 239000008280 blood Substances 0.000 description 1
• 230000014461 bone development Effects 0.000 description 1
• 210000001185 bone marrow Anatomy 0.000 description 1
• 230000004097 bone metabolism Effects 0.000 description 1
• 210000005013 brain tissue Anatomy 0.000 description 1
• 210000000481 breast Anatomy 0.000 description 1
• 239000000872 buffer Substances 0.000 description 1
• 230000000747 cardiac effect Effects 0.000 description 1
• 230000022900 cardiac muscle contraction Effects 0.000 description 1
• 150000003943 catecholamines Chemical class 0.000 description 1
• 150000001768 cations Chemical class 0.000 description 1
• 230000034303 cell budding Effects 0.000 description 1
• 230000022131 cell cycle Effects 0.000 description 1
• 210000000170 cell membrane Anatomy 0.000 description 1
• 210000003855 cell nucleus Anatomy 0.000 description 1
• 230000004663 cell proliferation Effects 0.000 description 1
• 230000010307 cell transformation Effects 0.000 description 1
• 210000003591 cerebellar nuclei Anatomy 0.000 description 1
• 238000012512 characterization method Methods 0.000 description 1
• 238000007385 chemical modification Methods 0.000 description 1
• 239000003638 chemical reducing agent Substances 0.000 description 1
• 210000003737 chromaffin cell Anatomy 0.000 description 1
• 230000002759 chromosomal effect Effects 0.000 description 1
• 229960002376 chymotrypsin Drugs 0.000 description 1
• 229930193282 clathrin Natural products 0.000 description 1
• 238000003776 cleavage reaction Methods 0.000 description 1
• 238000005094 computer simulation Methods 0.000 description 1
• 239000000470 constituent Substances 0.000 description 1
• 238000010276 construction Methods 0.000 description 1
• 230000008602 contraction Effects 0.000 description 1
• 230000000875 corresponding effect Effects 0.000 description 1
• 230000008878 coupling Effects 0.000 description 1
• 238000010168 coupling process Methods 0.000 description 1
• 238000005859 coupling reaction Methods 0.000 description 1
• 238000004132 cross linking Methods 0.000 description 1
• ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
• 235000018417 cysteine Nutrition 0.000 description 1
• 230000009089 cytolysis Effects 0.000 description 1
• 230000001461 cytolytic effect Effects 0.000 description 1
• 230000006378 damage Effects 0.000 description 1
• 230000034994 death Effects 0.000 description 1
• 210000004443 dendritic cell Anatomy 0.000 description 1
• 238000001212 derivatisation Methods 0.000 description 1
• 238000013461 design Methods 0.000 description 1
• 229960000633 dextran sulfate Drugs 0.000 description 1
• 206010012601 diabetes mellitus Diseases 0.000 description 1
• 238000002405 diagnostic procedure Methods 0.000 description 1
• 238000001085 differential centrifugation Methods 0.000 description 1
• 230000004069 differentiation Effects 0.000 description 1
• 238000006471 dimerization reaction Methods 0.000 description 1
• 238000009826 distribution Methods 0.000 description 1
• 231100000673 dose–response relationship Toxicity 0.000 description 1
• 238000004520 electroporation Methods 0.000 description 1
• 239000000839 emulsion Substances 0.000 description 1
• 210000001163 endosome Anatomy 0.000 description 1
• 210000002889 endothelial cell Anatomy 0.000 description 1
• 238000005516 engineering process Methods 0.000 description 1
• 230000002708 enhancing effect Effects 0.000 description 1
• 230000002255 enzymatic effect Effects 0.000 description 1
• 230000009144 enzymatic modification Effects 0.000 description 1
• 238000001976 enzyme digestion Methods 0.000 description 1
• 210000000981 epithelium Anatomy 0.000 description 1
• 210000003527 eukaryotic cell Anatomy 0.000 description 1
• 230000007717 exclusion Effects 0.000 description 1
• 230000001747 exhibiting effect Effects 0.000 description 1
• 238000002474 experimental method Methods 0.000 description 1
• 239000007850 fluorescent dye Substances 0.000 description 1
• 239000006260 foam Substances 0.000 description 1
• 238000005194 fractionation Methods 0.000 description 1
• 238000010230 functional analysis Methods 0.000 description 1
• 230000007849 functional defect Effects 0.000 description 1
• 229960003692 gamma aminobutyric acid Drugs 0.000 description 1
• 238000004817 gas chromatography Methods 0.000 description 1
• 238000001502 gel electrophoresis Methods 0.000 description 1
• 230000004190 glucose uptake Effects 0.000 description 1
• 235000013922 glutamic acid Nutrition 0.000 description 1
• 239000004220 glutamic acid Substances 0.000 description 1
• 210000004368 gonadotroph Anatomy 0.000 description 1
• 102000034345 heterotrimeric G proteins Human genes 0.000 description 1
• 108091006093 heterotrimeric G proteins Proteins 0.000 description 1
• 230000000971 hippocampal effect Effects 0.000 description 1
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
• 229920001519 homopolymer Polymers 0.000 description 1
• 230000003054 hormonal effect Effects 0.000 description 1
• 108091008039 hormone receptors Proteins 0.000 description 1
• 102000047635 human MRPS31 Human genes 0.000 description 1
• 230000000705 hypocalcaemia Effects 0.000 description 1
• 210000003016 hypothalamus Anatomy 0.000 description 1
• 238000003384 imaging method Methods 0.000 description 1
• 230000014726 immortalization of host cell Effects 0.000 description 1
• 208000003669 immune deficiency disease Diseases 0.000 description 1
• 230000028993 immune response Effects 0.000 description 1
• 230000003053 immunization Effects 0.000 description 1
• 238000002649 immunization Methods 0.000 description 1
• 230000007813 immunodeficiency Effects 0.000 description 1
• 230000016784 immunoglobulin production Effects 0.000 description 1
• 238000000126 in silico method Methods 0.000 description 1
• 238000000099 in vitro assay Methods 0.000 description 1
• 210000003000 inclusion body Anatomy 0.000 description 1
• 230000006882 induction of apoptosis Effects 0.000 description 1
• 208000030603 inherited susceptibility to asthma Diseases 0.000 description 1
• 230000005764 inhibitory process Effects 0.000 description 1
• 238000002347 injection Methods 0.000 description 1
• 239000007924 injection Substances 0.000 description 1
• 229910052500 inorganic mineral Inorganic materials 0.000 description 1
• 229940125396 insulin Drugs 0.000 description 1
• 210000004020 intracellular membrane Anatomy 0.000 description 1
• 230000029225 intracellular protein transport Effects 0.000 description 1
• 238000005342 ion exchange Methods 0.000 description 1
• 238000001155 isoelectric focusing Methods 0.000 description 1
• 231100000518 lethal Toxicity 0.000 description 1
• 230000001665 lethal effect Effects 0.000 description 1
• 239000007788 liquid Substances 0.000 description 1
• 230000004807 localization Effects 0.000 description 1
• 206010025135 lupus erythematosus Diseases 0.000 description 1
• 230000002101 lytic effect Effects 0.000 description 1
• 230000007257 malfunction Effects 0.000 description 1
• 230000036210 malignancy Effects 0.000 description 1
• 229910052748 manganese Inorganic materials 0.000 description 1
• 239000011572 manganese Substances 0.000 description 1
• 238000004949 mass spectrometry Methods 0.000 description 1
• 239000011159 matrix material Substances 0.000 description 1
• 230000010534 mechanism of action Effects 0.000 description 1
• 230000002503 metabolic effect Effects 0.000 description 1
• 230000004060 metabolic process Effects 0.000 description 1
• 244000005700 microbiome Species 0.000 description 1
• 230000029115 microtubule polymerization Effects 0.000 description 1
• 230000005012 migration Effects 0.000 description 1
• 238000013508 migration Methods 0.000 description 1
• 239000011707 mineral Substances 0.000 description 1
• 210000003470 mitochondria Anatomy 0.000 description 1
• 230000002438 mitochondrial effect Effects 0.000 description 1
• 239000003226 mitogen Substances 0.000 description 1
• 238000000302 molecular modelling Methods 0.000 description 1
• 201000006417 multiple sclerosis Diseases 0.000 description 1
• 210000003205 muscle Anatomy 0.000 description 1
• 210000000066 myeloid cell Anatomy 0.000 description 1
• 210000004165 myocardium Anatomy 0.000 description 1
• 108010065781 myosin light chain 2 Proteins 0.000 description 1
• 210000004897 n-terminal region Anatomy 0.000 description 1
• 210000000581 natural killer T-cell Anatomy 0.000 description 1
• 230000009826 neoplastic cell growth Effects 0.000 description 1
• 230000001613 neoplastic effect Effects 0.000 description 1
• 230000010309 neoplastic transformation Effects 0.000 description 1
• 210000004412 neuroendocrine cell Anatomy 0.000 description 1
• 210000002569 neuron Anatomy 0.000 description 1
• 230000007996 neuronal plasticity Effects 0.000 description 1
• 208000004235 neutropenia Diseases 0.000 description 1
• 108091008046 non-receptor tyrosine kinases Proteins 0.000 description 1
• 102000037979 non-receptor tyrosine kinases Human genes 0.000 description 1
• 230000025308 nuclear transport Effects 0.000 description 1
• 239000003921 oil Substances 0.000 description 1
• 235000019198 oils Nutrition 0.000 description 1
• 210000004248 oligodendroglia Anatomy 0.000 description 1
• 102000027450 oncoproteins Human genes 0.000 description 1
• 108091008819 oncoproteins Proteins 0.000 description 1
• 210000000056 organ Anatomy 0.000 description 1
• 230000005305 organ development Effects 0.000 description 1
• 208000028776 osteoblastic osteosarcoma Diseases 0.000 description 1
• 201000008968 osteosarcoma Diseases 0.000 description 1
• 230000008506 pathogenesis Effects 0.000 description 1
• 229940111202 pepsin Drugs 0.000 description 1
• 210000005259 peripheral blood Anatomy 0.000 description 1
• 239000011886 peripheral blood Substances 0.000 description 1
• 208000033808 peripheral neuropathy Diseases 0.000 description 1
• 239000012071 phase Substances 0.000 description 1
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
• 108010079892 phosphoglycerol kinase Proteins 0.000 description 1
• 230000000243 photosynthetic effect Effects 0.000 description 1
• 230000004962 physiological condition Effects 0.000 description 1
• 239000013600 plasmid vector Substances 0.000 description 1
• 208000004521 platelet storage pool deficiency Diseases 0.000 description 1
• 229920001983 poloxamer Polymers 0.000 description 1
• 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
• 229920000447 polyanionic polymer Polymers 0.000 description 1
• 229920005862 polyol Polymers 0.000 description 1
• 150000003077 polyols Chemical class 0.000 description 1
• 229920001184 polypeptide Polymers 0.000 description 1
• 239000011148 porous material Substances 0.000 description 1
• 230000007542 postnatal development Effects 0.000 description 1
• 230000002028 premature Effects 0.000 description 1
• 238000002360 preparation method Methods 0.000 description 1
• 230000005522 programmed cell death Effects 0.000 description 1
• 230000000750 progressive effect Effects 0.000 description 1
• 230000009465 prokaryotic expression Effects 0.000 description 1
• 230000002062 proliferating effect Effects 0.000 description 1
• 230000001737 promoting effect Effects 0.000 description 1
• 230000000644 propagated effect Effects 0.000 description 1
• 230000000069 prophylactic effect Effects 0.000 description 1
• 235000019419 proteases Nutrition 0.000 description 1
• 238000000159 protein binding assay Methods 0.000 description 1
• 235000004252 protein component Nutrition 0.000 description 1
• 108020001580 protein domains Proteins 0.000 description 1
• 230000006916 protein interaction Effects 0.000 description 1
• 230000026447 protein localization Effects 0.000 description 1
• 229940076155 protein modulator Drugs 0.000 description 1
• 238000001742 protein purification Methods 0.000 description 1
• 230000003161 proteinsynthetic effect Effects 0.000 description 1
• 230000002797 proteolythic effect Effects 0.000 description 1
• 230000006337 proteolytic cleavage Effects 0.000 description 1
• 210000000449 purkinje cell Anatomy 0.000 description 1
• 238000000163 radioactive labelling Methods 0.000 description 1
• 108091008598 receptor tyrosine kinases Proteins 0.000 description 1
• 102000005962 receptors Human genes 0.000 description 1
• 238000010188 recombinant method Methods 0.000 description 1
• 238000006722 reduction reaction Methods 0.000 description 1
• 230000025915 regulation of apoptotic process Effects 0.000 description 1
• 230000022983 regulation of cell cycle Effects 0.000 description 1
• 230000004161 regulation of exocytosis Effects 0.000 description 1
• 230000023604 regulation of skeletal muscle contraction Effects 0.000 description 1
• 239000003488 releasing hormone Substances 0.000 description 1
• 230000008521 reorganization Effects 0.000 description 1
• 206010039073 rheumatoid arthritis Diseases 0.000 description 1
• 206010039083 rhinitis Diseases 0.000 description 1
• 210000004708 ribosome subunit Anatomy 0.000 description 1
• 230000007017 scission Effects 0.000 description 1
• 230000003248 secreting effect Effects 0.000 description 1
• 230000028327 secretion Effects 0.000 description 1
• 210000004739 secretory vesicle Anatomy 0.000 description 1
• 102000034285 signal transducing proteins Human genes 0.000 description 1
• 108091006024 signal transducing proteins Proteins 0.000 description 1
• 238000002741 site-directed mutagenesis Methods 0.000 description 1
• 210000002027 skeletal muscle Anatomy 0.000 description 1
• 150000003384 small molecules Chemical class 0.000 description 1
• 239000011734 sodium Substances 0.000 description 1
• 239000012279 sodium borohydride Substances 0.000 description 1
• 229910000033 sodium borohydride Inorganic materials 0.000 description 1
• 238000000527 sonication Methods 0.000 description 1
• 230000009870 specific binding Effects 0.000 description 1
• 210000000952 spleen Anatomy 0.000 description 1
• 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
• 239000007858 starting material Substances 0.000 description 1
• 230000000638 stimulation Effects 0.000 description 1
• 230000035882 stress Effects 0.000 description 1
• 210000000225 synapse Anatomy 0.000 description 1
• 230000005062 synaptic transmission Effects 0.000 description 1
• 210000002504 synaptic vesicle Anatomy 0.000 description 1
• 108060008004 synaptotagmin Proteins 0.000 description 1
• 102000003137 synaptotagmin Human genes 0.000 description 1
• 208000011580 syndromic disease Diseases 0.000 description 1
• 230000009885 systemic effect Effects 0.000 description 1
• 210000001587 telencephalon Anatomy 0.000 description 1
• 230000002381 testicular Effects 0.000 description 1
• CBXCPBUEXACCNR-UHFFFAOYSA-N tetraethylammonium Chemical compound CC[N+](CC)(CC)CC CBXCPBUEXACCNR-UHFFFAOYSA-N 0.000 description 1
• 238000002560 therapeutic procedure Methods 0.000 description 1
• 230000004797 therapeutic response Effects 0.000 description 1
• 230000002992 thymic effect Effects 0.000 description 1
• 239000005495 thyroid hormone Substances 0.000 description 1
• 229940036555 thyroid hormone Drugs 0.000 description 1
• 238000001890 transfection Methods 0.000 description 1
• 230000001131 transforming effect Effects 0.000 description 1
• 230000009261 transgenic effect Effects 0.000 description 1
• 230000005945 translocation Effects 0.000 description 1
• 108010013477 troponin-tropomyosin complex Proteins 0.000 description 1
• 239000012588 trypsin Substances 0.000 description 1
• 229960001322 trypsin Drugs 0.000 description 1
• 229960004799 tryptophan Drugs 0.000 description 1
• ZHSGGJXRNHWHRS-VIDYELAYSA-N tunicamycin Chemical compound O([C@H]1[C@@H]([C@H]([C@@H](O)[C@@H](CC(O)[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C(NC(=O)C=C2)=O)O)O1)O)NC(=O)/C=C/CC(C)C)[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1NC(C)=O ZHSGGJXRNHWHRS-VIDYELAYSA-N 0.000 description 1
• MEYZYGMYMLNUHJ-UHFFFAOYSA-N tunicamycin Natural products CC(C)CCCCCCCCCC=CC(=O)NC1C(O)C(O)C(CC(O)C2OC(C(O)C2O)N3C=CC(=O)NC3=O)OC1OC4OC(CO)C(O)C(O)C4NC(=O)C MEYZYGMYMLNUHJ-UHFFFAOYSA-N 0.000 description 1
• 238000002424 x-ray crystallography Methods 0.000 description 1