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CA2321169C - Enzymes modifiees et leur utilisation pour la synthese peptidique - Google Patents

Enzymes modifiees et leur utilisation pour la synthese peptidique Download PDF

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Publication number
CA2321169C
CA2321169C CA2321169A CA2321169A CA2321169C CA 2321169 C CA2321169 C CA 2321169C CA 2321169 A CA2321169 A CA 2321169A CA 2321169 A CA2321169 A CA 2321169A CA 2321169 C CA2321169 C CA 2321169C
Authority
CA
Canada
Prior art keywords
enzyme
modified
esterase
amino acid
cysteine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2321169A
Other languages
English (en)
Other versions
CA2321169A1 (fr
Inventor
J. Bryan Jones
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of CA2321169A1 publication Critical patent/CA2321169A1/fr
Application granted granted Critical
Publication of CA2321169C publication Critical patent/CA2321169C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C07K1/023—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution using racemisation inhibiting agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06026—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atom, i.e. Gly or Ala
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06078—Dipeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06086—Dipeptides with the first amino acid being basic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06104—Dipeptides with the first amino acid being acidic
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/836—Bacillus licheniformis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

La présente invention concerne des enzymes modifiées, un ou plusieurs restes d'acides aminés issus d'une enzyme étant remplacés par des restes de cystéine. Dans ces enzymes, certains des restes de cystéine au moins sont modifiés en remplaçant un hydrogène du thiol dans le reste de cystéine par une chaîne latérale thiolique pour former une enzyme modifiée, l'enzyme modifiée présentant une grande activité estérasique et une faible activité amidasique. L'invention concerne également un procédé permettant de produire ces enzymes modifiées. La présente invention concerne enfin un procédé d'utilisation des enzymes dans la synthèse peptidique.
CA2321169A 1998-01-23 1999-01-21 Enzymes modifiees et leur utilisation pour la synthese peptidique Expired - Fee Related CA2321169C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US7226598P 1998-01-23 1998-01-23
US7235198P 1998-01-23 1998-01-23
US60/072,351 1998-01-23
US60/072,265 1998-01-23
PCT/US1999/001413 WO1999037324A1 (fr) 1998-01-23 1999-01-21 Enzymes modifiees et leur utilisation pour la synthese peptidique

Publications (2)

Publication Number Publication Date
CA2321169A1 CA2321169A1 (fr) 1999-07-29
CA2321169C true CA2321169C (fr) 2011-08-02

Family

ID=26753173

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2321169A Expired - Fee Related CA2321169C (fr) 1998-01-23 1999-01-21 Enzymes modifiees et leur utilisation pour la synthese peptidique

Country Status (9)

Country Link
US (2) US6395532B1 (fr)
EP (1) EP1047445B1 (fr)
JP (2) JP2002500874A (fr)
KR (1) KR20010040397A (fr)
AU (1) AU739432C (fr)
CA (1) CA2321169C (fr)
DK (1) DK1047445T3 (fr)
NZ (1) NZ506346A (fr)
WO (1) WO1999037324A1 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
KR20010034697A (ko) 1998-03-26 2001-04-25 데이비드 엠 모이어 아미노산 치환을 갖는 세린 프로테아제 변이체
AU749383B2 (en) 1998-07-02 2002-06-27 Genencor International, Inc. Chemically modified proteins with a carbohydrate moiety
AU772427B2 (en) * 1998-11-10 2004-04-29 Genencor International, Inc. Chemically modified mutant serine hydrolases show improved catalytic activity and chiral selectivity
MXPA01010896A (es) * 1999-04-28 2003-06-24 Genencor Int Antagonistas cataliticos especificamente dirigidos al objetivo y usos de los mismos.
US6627744B2 (en) 1999-07-02 2003-09-30 Genencor International, Inc. Synthesis of glycodendrimer reagents
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
BR0012660A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
CN1373802A (zh) 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
BR0012692A (pt) * 1999-07-22 2002-04-09 Procter & Gamble Conjugado de protease, composição de limpeza e composição de tratamento pessoal
BR0316488A (pt) * 2002-11-25 2005-10-11 Zealand Pharma As Métodos para modular uma comunicação de junção lacunar em uma população de células, e para tratar um paciente tendo ou sob risco de desenvolver uma condição patológica envolvendo comunicação de junção lacunar deficiente, uso de um peptìdeo, e, composição farmacêutica
US7087438B2 (en) * 2004-07-26 2006-08-08 Infineon Technologies Ag Encapsulation of conductive lines of semiconductor devices
EP2596087B1 (fr) 2010-07-22 2015-12-16 Unilever PLC Combinaisons de rhamnolipides et d'enzymes pour nettoyage amélioré

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58144105A (ja) * 1982-02-12 1983-08-27 Kurabo Ind Ltd スケ−ル除去獣毛繊維の製法
US5244791A (en) * 1984-05-29 1993-09-14 Genecor International, Inc. Methods of ester hydrolysis
US5155033A (en) 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
US5037741A (en) * 1986-08-18 1991-08-06 The Coca Cola Company Enzymatic method for the synthesis and separation of peptides
CN1056187C (zh) 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
DK97190D0 (da) 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
US5629173A (en) * 1990-08-09 1997-05-13 Genentech, Inc. Methods of use of serine protease variants having peptide ligase activity
EP0542931B1 (fr) * 1990-08-09 1999-12-08 Genentech, Inc. Variantes de serine-proteases presentant une activite de ligase peptidique
US5316935A (en) 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
GB9416841D0 (en) * 1994-08-19 1994-10-12 Finnfeeds Int Ltd An enzyme feed additive and animal feed including it
US5837516A (en) 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues

Also Published As

Publication number Publication date
US6395532B1 (en) 2002-05-28
JP2010029195A (ja) 2010-02-12
AU2337199A (en) 1999-08-09
EP1047445A1 (fr) 2000-11-02
NZ506346A (en) 2003-06-30
US6576454B2 (en) 2003-06-10
WO1999037324A1 (fr) 1999-07-29
AU739432C (en) 2002-05-16
AU739432B2 (en) 2001-10-11
DK1047445T3 (da) 2013-11-11
US20020137177A1 (en) 2002-09-26
CA2321169A1 (fr) 1999-07-29
EP1047445A4 (fr) 2001-09-12
KR20010040397A (ko) 2001-05-15
JP2002500874A (ja) 2002-01-15
EP1047445B1 (fr) 2013-08-28

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Effective date: 20170123