CA2301285A1 - Sequences genetiques, procedes et dispositifs de diagnostic et/ou de quantification pour l'identification de souches de staphylocoques - Google Patents
Sequences genetiques, procedes et dispositifs de diagnostic et/ou de quantification pour l'identification de souches de staphylocoques Download PDFInfo
- Publication number
- CA2301285A1 CA2301285A1 CA002301285A CA2301285A CA2301285A1 CA 2301285 A1 CA2301285 A1 CA 2301285A1 CA 002301285 A CA002301285 A CA 002301285A CA 2301285 A CA2301285 A CA 2301285A CA 2301285 A1 CA2301285 A1 CA 2301285A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleotide sequence
- base pairs
- seq
- fema
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241000295644 Staphylococcaceae Species 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 24
- 230000002068 genetic effect Effects 0.000 title claims description 14
- 238000011002 quantification Methods 0.000 title claims description 10
- 239000002773 nucleotide Substances 0.000 claims abstract description 57
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 57
- 108091034117 Oligonucleotide Proteins 0.000 claims abstract description 38
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims abstract description 18
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 39
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- 101150022703 femA gene Proteins 0.000 description 42
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- 239000013598 vector Substances 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 10
- 241000191963 Staphylococcus epidermidis Species 0.000 description 10
- 241000192087 Staphylococcus hominis Species 0.000 description 9
- 241001147691 Staphylococcus saprophyticus Species 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 241000191984 Staphylococcus haemolyticus Species 0.000 description 7
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- 108090000765 processed proteins & peptides Proteins 0.000 description 6
- 108091035707 Consensus sequence Proteins 0.000 description 5
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- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108091093088 Amplicon Proteins 0.000 description 4
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 4
- 241000191940 Staphylococcus Species 0.000 description 4
- 241001134656 Staphylococcus lugdunensis Species 0.000 description 4
- 238000003205 genotyping method Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 229960003085 meticillin Drugs 0.000 description 4
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 3
- 108010013639 Peptidoglycan Proteins 0.000 description 3
- 206010041925 Staphylococcal infections Diseases 0.000 description 3
- 241001147736 Staphylococcus capitis Species 0.000 description 3
- 241000192099 Staphylococcus schleiferi Species 0.000 description 3
- 241000191973 Staphylococcus xylosus Species 0.000 description 3
- 150000001413 amino acids Chemical group 0.000 description 3
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- 238000007403 mPCR Methods 0.000 description 3
- 101150008979 mecA gene Proteins 0.000 description 3
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- 238000012163 sequencing technique Methods 0.000 description 3
- 101100502610 Caenorhabditis elegans fem-2 gene Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
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- 229910019142 PO4 Inorganic materials 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
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- 238000010369 molecular cloning Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- MXHCPCSDRGLRER-UHFFFAOYSA-N pentaglycine Chemical group NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(O)=O MXHCPCSDRGLRER-UHFFFAOYSA-N 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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- 229960003165 vancomycin Drugs 0.000 description 1
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention se rapporte à des oligonucléotides pour l'identification spécifique d'espèces de staphylocoques dont la séquence nucléotide présente entre 15 et 350 paires de base, de préférence entre 15 et 45 paires de base, obtenues par "consensus" de la séquence nucléotide femA (CNS) de la figure ou de son brin complémentaire. L'invention concerne également un procédé et un dispositif de diagnostic utilisant lesdits oligonucléotides pour l'identification de divers types de souches d'espèces de staphylocoques.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97870146.4 | 1997-09-26 | ||
| EP97870146 | 1997-09-26 | ||
| PCT/BE1998/000141 WO1999016780A2 (fr) | 1997-09-26 | 1998-09-28 | Sequences genetiques, procedes et dispositifs de diagnostic et/ou de quantification pour l'identification de souches de staphylocoques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2301285A1 true CA2301285A1 (fr) | 1999-04-08 |
Family
ID=8231045
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002301285A Abandoned CA2301285A1 (fr) | 1997-09-26 | 1998-09-28 | Sequences genetiques, procedes et dispositifs de diagnostic et/ou de quantification pour l'identification de souches de staphylocoques |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1017850A2 (fr) |
| JP (1) | JP2001518283A (fr) |
| CA (1) | CA2301285A1 (fr) |
| WO (1) | WO1999016780A2 (fr) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1096024A1 (fr) * | 1999-10-28 | 2001-05-02 | Remacle, José | Méthode et trousse pour le criblage et/ou la quantification de séquences d' acides nucléiques multiples homologues avec des ensembles de sondes |
| US7205104B2 (en) * | 2000-03-24 | 2007-04-17 | Eppendorf Array Technologies Sa (Eat) | Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays |
| US7829313B2 (en) | 2000-03-24 | 2010-11-09 | Eppendorf Array Technologies | Identification and quantification of a plurality of biological (micro)organisms or their components |
| US7202026B2 (en) * | 2000-03-24 | 2007-04-10 | Eppendorf Array Technologies Sa (Eat) | Identification of a large number of biological (micro)organisms groups at different levels by their detection on a same array |
| EP1136566A1 (fr) * | 2000-03-24 | 2001-09-26 | Facultés Universitaires Notre-Dame de la Paix | Procédé et trousse pour la détection et/ou la quantification d'acides nucléiques homologues par un ensemble d'ADN |
| US7875442B2 (en) | 2000-03-24 | 2011-01-25 | Eppendorf Array Technologies | Identification and quantification of a plurality of biological (micro)organisms or their components |
| US7090997B2 (en) * | 2000-10-26 | 2006-08-15 | Eisai Co., Ltd. | Diagnostic agent and test method for colon cancer using tannase as index |
| AU2013231102B2 (en) * | 2001-03-02 | 2016-04-28 | Ibis Biosciences, Inc. | Methods for rapid identification of pathogens in humans and animals |
| US7666588B2 (en) | 2001-03-02 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy |
| JP2004531251A (ja) * | 2001-03-15 | 2004-10-14 | シュレンツェル，ジャック | メチシリン耐性黄色ブドウ球菌（ｍｒｓａ）の直接検出の方法 |
| CA2508726A1 (fr) | 2002-12-06 | 2004-07-22 | Isis Pharmaceuticals, Inc. | Procedes d'identification rapide de pathogenes chez l'homme et les betes |
| WO2005017202A2 (fr) | 2003-05-13 | 2005-02-24 | Gen-Probe Incorporated | Procede et trousse permettant l'identification de micro-organismes resistants aux antibiotiques |
| US7338763B2 (en) | 2004-06-02 | 2008-03-04 | Eppendorf Array Technologies S.A. | Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays |
| SG11202002860VA (en) * | 2017-10-12 | 2020-04-29 | Mitsui Chemicals Inc | mecA GENE AMPLIFICATION PRIMER PAIR, mecA GENE DETECTION KIT AND mecA GENE DETECTION METHOD |
| CN112195225A (zh) * | 2020-10-14 | 2021-01-08 | 南通大学附属医院 | 基于耐药基因MecA检测耐甲氧西林葡萄球菌的方法 |
| US20240197820A1 (en) | 2021-06-02 | 2024-06-20 | Syngulon S.A. | Bacteriocin for new application |
| US20240285726A1 (en) | 2021-06-02 | 2024-08-29 | Syngulon S.A. | Bacteriocin for new application |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2075423A1 (fr) * | 1991-08-13 | 1993-02-14 | Paul Luther Skatrud | Methode de detection rapide des staphylocoques resistant a la methicilline |
| EP0625575A3 (fr) * | 1993-04-30 | 1995-02-22 | Lilly Co Eli | Gène Fem A de staphylococcus épidermis, protéine Fem A, et vecteurs et micro-organismes contenant le gène Fem A. |
-
1998
- 1998-09-28 EP EP98944907A patent/EP1017850A2/fr not_active Withdrawn
- 1998-09-28 CA CA002301285A patent/CA2301285A1/fr not_active Abandoned
- 1998-09-28 WO PCT/BE1998/000141 patent/WO1999016780A2/fr not_active Ceased
- 1998-09-28 JP JP2000513862A patent/JP2001518283A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1017850A2 (fr) | 2000-07-12 |
| WO1999016780A2 (fr) | 1999-04-08 |
| WO1999016780A3 (fr) | 1999-08-05 |
| JP2001518283A (ja) | 2001-10-16 |
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