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CA2393016A1 - Triphasic bioreactor and process for gas effluent treatment - Google Patents

Triphasic bioreactor and process for gas effluent treatment Download PDF

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Publication number
CA2393016A1
CA2393016A1 CA002393016A CA2393016A CA2393016A1 CA 2393016 A1 CA2393016 A1 CA 2393016A1 CA 002393016 A CA002393016 A CA 002393016A CA 2393016 A CA2393016 A CA 2393016A CA 2393016 A1 CA2393016 A1 CA 2393016A1
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CA
Canada
Prior art keywords
reaction chamber
gas
liquid
biocatalysts
bioreactor according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002393016A
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French (fr)
Other versions
CA2393016C (en
Inventor
Carmen Parent
Frederic Dutil
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Saipem SpA
Original Assignee
Co2 Solutions Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002353307A external-priority patent/CA2353307A1/en
Application filed by Co2 Solutions Inc filed Critical Co2 Solutions Inc
Priority to CA2393016A priority Critical patent/CA2393016C/en
Priority to CA2714304A priority patent/CA2714304C/en
Priority to AT02787316T priority patent/ATE353245T1/en
Priority to DE60218071T priority patent/DE60218071D1/en
Priority to PCT/CA2002/002029 priority patent/WO2004007058A1/en
Priority to AU2002351643A priority patent/AU2002351643A1/en
Priority to EP02787316A priority patent/EP1521629B1/en
Publication of CA2393016A1 publication Critical patent/CA2393016A1/en
Publication of CA2393016C publication Critical patent/CA2393016C/en
Application granted granted Critical
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J8/00Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes
    • B01J8/18Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes with fluidised particles
    • B01J8/20Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes with fluidised particles with liquid as a fluidising medium
    • B01J8/22Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes with fluidised particles with liquid as a fluidising medium gas being introduced into the liquid
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/74General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84Biological processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J8/00Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes
    • B01J8/18Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes with fluidised particles
    • B01J8/20Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes with fluidised particles with liquid as a fluidising medium
    • B01J8/22Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes with fluidised particles with liquid as a fluidising medium gas being introduced into the liquid
    • B01J8/222Chemical or physical processes in general, conducted in the presence of fluids and solid particles; Apparatus for such processes with fluidised particles with liquid as a fluidising medium gas being introduced into the liquid in the presence of a rotating device only
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02Membranes; Filters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/02Percolation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/59Biological synthesis; Biological purification

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Combustion & Propulsion (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A triphasic bioreactor, for physico-chemically treating a gas is disclosed.
The triphasic bioreactor comprises a reaction chamber with a liquid and biocatalysts in suspension in the liquid, for catalyzing a reaction between the gas and the liquid to obtain a treated gas and a solution containing a reaction product. A gas bubbling means is provided in the reaction bubbling the gas to be treated into the liquid thereby dissolving the gas into the liquid and increasing a pressure inside the reaction chamber. The bioreactor further comprises a liquid inlet in fluid communication with the reaction chamber for receiving the liquid and filling the reaction chamber, a liquid outlet in fluid communication with the reaction chamber for releasing the solution and a gas outlet in fluid communication with the reaction chamber to release the treated gas. The bioreactor further comprises a retention device to retain the biocatalysts in the reaction chamber. The invention also concerns a process using the triphasic bioreactor. The triphasic bioreactor may advantageously be used for removing carbonic dioxide from a CO2-containing gas

Claims (21)

1. A triphasic bioreactor for physico-chemically treating a gas, comprising:
-a reaction chamber filled with a liquid;
-biocatalysts in suspension in the liquid for catalyzing a reaction between the gas and the liquid to obtain a treated gas and a solution containing a reaction product;
-a liquid inlet in fluid communication with the reaction chamber for receiving the liquid and filling the reaction chamber;
-a gas bubbling means in the reaction chamber for bubbling the gas to be treated into the liquid thereby dissolving the gas into the liquid and increasing a pressure inside the reaction chamber;
-a liquid outlet in fluid communication with the reaction chamber for releasing said solution containing the reaction product;
- a gas outlet in fluid communication with the reaction chamber to release said treated gas; and -a retention device to retain the biocatalysts in the reaction chamber.
2. A triphasic bioreactor according to claim 1, comprising a pressure regulating valve to control a pressure-created by the gas bubbled within the reaction chamber.
3. A triphasic bioreactor according to claim 1, wherein the pressure regulating valve is located in the gas outlet.
4. A triphasic bioreactor according claim 1, wherein the biocatalysts are selected from the group consisting of enzymes, liposomes, microoganisms, animal cells, plant cells and a combination thereof.
5. A triphasic bioreactor according to claim 4, wherein the retention device comprises a filter having pores with a smaller diameter than a diameter of the biocatalysts.
6. A triphasic bioreactor according to claim 5, wherein the filter is a membrane filter.
7. A triphasic bioreactor according to claim 6, wherein the membrane filter is located inside the reaction chamber upstream from the liquid outlet.
8. A triphasic bioreactor according to claim 7, comprising a closed loop circuit including a pump to circulate liquid tangentially to the membrane.
9. A triphasic bioreactor according to claim 6, wherein the membrane filter is located outside the reaction chamber and the retention device further comprises:
-a first piping means for piping liquid, which contains biocatalysts and reaction products, from the liquid outlet of the reaction chamber to the membrane filter where a permeate liquid containing the reaction products is separated from a retentate liquid containing the biocatalysts; and -a second piping means for piping the retentate liquid to the liquid inlet of the bioreactor.
10. A triphasic bioreactor according to claim 1, wherein the biocatalysts are present in the bioreactor as complexes of said biocatalysts.
11. A triphasic bioreactor according to claim 10, wherein said complexes of biocatalysts are selected from the group consisting of biocatalysts entrapped in a porous substrate, biocatalysts immobilized on a solid substrate and biocatalysts linked in a network of molecules in suspension.
12. A triphasic bioreactor according to claim 1, wherein the gas bubbling means comprise:
- a gas inlet of the reaction chamber to receive the gas effluent;
- a bubbler located in a bottom portion of the reaction chamber having:
a gas inlet connected to the gas inlet of the reaction chamber; and a plurality of gas outlets to diffuse the gas in the reaction chamber;
and - a pipe to connect the gas inlet of the reaction chamber to the gas inlet of the bubbler.
13. A triphasic bioreactor according to claim 1, wherein the gas effluent contains carbon dioxide, the liquid is an aqueous liquid and the biocatalysts are enzymes capable of catalyzing the chemical conversion of the dissolved carbon dioxide into an aqueous solution containing hydrogen ions and bicarbonate ions.
14. A triphasic bioreactor according to claim 13 wherein, the enzymes are carbonic anhydrase.
15. A triphasic bioreactor according to claim 1, comprising a valve at the liquid outlet and/or at the liquid inlet for regulating a flow of liquid in and out of the reaction chamber.
16. A bioreactor according to claim 1, comprising a sampling means for sampling and analyzing liquid from the reaction chamber.
17. A bioreactor according to claim 1, comprising an additional reaction chamber as defined in claim 1, in series with said reaction chamber, hereinafter referred as the first reaction chamber, to further treat said treated gas.
18. A bioreactor according to claim 17, wherein the biocatalysts filling the first reaction chamber are different from the biocatalysts filling the additional reaction chamber.
19. A triphasic process for physico-chemically treating a gas, comprising the steps of:
a) filling a reaction chamber with a liquid and biocatalysts suspended in the liquid;
b) bubbling the gas to be treated into the reaction chamber to dissolve the gas into the liquid and to increase a pressure within the reaction chamber, thereby promoting a catalyzed reaction between the liquid and the gas to obtain a treated gas and a solution containing a reaction product;
c) releasing by pressure the solution obtained in step b) from the reaction chamber whilst retaining the biocatalysts within the reaction chamber, d) during steps b) and c) controlling the pressure with in the reaction chamber and releasing the treated gas obtained in step b).
20. A process according to claim 19, wherein step c) of retaining comprises an ultrafiltration of the solution obtained in step b).
21. A process according claim 20, wherein the ultrafiltration is conducted inside or outside the reaction chamber.
CA2393016A 2001-07-13 2002-07-11 Triphasic bioreactor and process for gas effluent treatment Expired - Lifetime CA2393016C (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA2393016A CA2393016C (en) 2001-07-13 2002-07-11 Triphasic bioreactor and process for gas effluent treatment
CA2714304A CA2714304C (en) 2001-07-13 2002-07-11 Carbonic anhydrase bioreactor and process for co2-containing gas effluent treatment
PCT/CA2002/002029 WO2004007058A1 (en) 2002-07-11 2002-12-20 Triphasic bioreactor and process for gas effluent treatment
DE60218071T DE60218071D1 (en) 2002-07-11 2002-12-20 THREE PHASE BIOREACTOR AND METHOD FOR TREATING EXHAUST GASES
AT02787316T ATE353245T1 (en) 2002-07-11 2002-12-20 THREE-PHASE BIOREACTOR AND METHOD FOR TREATING EXHAUST GASES
AU2002351643A AU2002351643A1 (en) 2002-07-11 2002-12-20 Triphasic bioreactor and process for gas effluent treatment
EP02787316A EP1521629B1 (en) 2002-07-11 2002-12-20 Triphasic bioreactor and process for gas effluent treatment

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CA002353307A CA2353307A1 (en) 2001-07-13 2001-07-13 Device and procedure for processing gaseous effluents
CA2,353,307 2001-07-13
CA2393016A CA2393016C (en) 2001-07-13 2002-07-11 Triphasic bioreactor and process for gas effluent treatment

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2714304A Division CA2714304C (en) 2001-07-13 2002-07-11 Carbonic anhydrase bioreactor and process for co2-containing gas effluent treatment

Publications (2)

Publication Number Publication Date
CA2393016A1 true CA2393016A1 (en) 2003-01-13
CA2393016C CA2393016C (en) 2011-01-04

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CA2714304A Expired - Lifetime CA2714304C (en) 2001-07-13 2002-07-11 Carbonic anhydrase bioreactor and process for co2-containing gas effluent treatment
CA2393016A Expired - Lifetime CA2393016C (en) 2001-07-13 2002-07-11 Triphasic bioreactor and process for gas effluent treatment

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CA2714304A Expired - Lifetime CA2714304C (en) 2001-07-13 2002-07-11 Carbonic anhydrase bioreactor and process for co2-containing gas effluent treatment

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7521217B2 (en) 2005-04-21 2009-04-21 Co2 Solution, Inc. Carbonic anhydrase having increased stability under high temperature conditions
WO2014000113A1 (en) * 2012-06-29 2014-01-03 Co2 Solutions Inc. Techniques for biocatalytic treatment of co2-containing gas and for separation of biocatalyst from ion loaded streams
WO2014066999A1 (en) 2012-10-29 2014-05-08 Co2 Solutions Inc. Techniques for co2 capture using sulfurihydrogenibium sp. carbonic anhydrase
WO2016029316A1 (en) 2014-08-27 2016-03-03 Co2 Solutions Inc. Co2 capture methods using thermovibrio ammonificans carbonic anhydrase

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013067648A1 (en) * 2011-11-11 2013-05-16 Co2 Solutions Inc. Co2 capture with carbonic anhydrase and membrane filtration

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7521217B2 (en) 2005-04-21 2009-04-21 Co2 Solution, Inc. Carbonic anhydrase having increased stability under high temperature conditions
US8263383B2 (en) 2005-04-21 2012-09-11 CO2 Solutions, Inc. Carbonic anhydrase having increased stability under high temperature conditions
WO2014000113A1 (en) * 2012-06-29 2014-01-03 Co2 Solutions Inc. Techniques for biocatalytic treatment of co2-containing gas and for separation of biocatalyst from ion loaded streams
WO2014066999A1 (en) 2012-10-29 2014-05-08 Co2 Solutions Inc. Techniques for co2 capture using sulfurihydrogenibium sp. carbonic anhydrase
US9968885B2 (en) 2012-10-29 2018-05-15 Co2 Solutions Inc. Techniques for CO2 capture using sulfurihydrogenibium sp. carbonic anhydrase
WO2016029316A1 (en) 2014-08-27 2016-03-03 Co2 Solutions Inc. Co2 capture methods using thermovibrio ammonificans carbonic anhydrase
US10926220B2 (en) 2014-08-27 2021-02-23 Saipem S.P.A. CO2 capture methods using Thermovibrio ammonificans carbonic anhydrase
EP4011484A1 (en) 2014-08-27 2022-06-15 SAIPEM S.p.A. Co2 capture methods using thermovibrio ammonificans carbonic anhydrase

Also Published As

Publication number Publication date
CA2714304A1 (en) 2003-01-13
CA2393016C (en) 2011-01-04
CA2714304C (en) 2013-07-09

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