[go: up one dir, main page]

CA2381139A1 - Procede pour produire une proteine formant un canal - Google Patents

Procede pour produire une proteine formant un canal Download PDF

Info

Publication number
CA2381139A1
CA2381139A1 CA002381139A CA2381139A CA2381139A1 CA 2381139 A1 CA2381139 A1 CA 2381139A1 CA 002381139 A CA002381139 A CA 002381139A CA 2381139 A CA2381139 A CA 2381139A CA 2381139 A1 CA2381139 A1 CA 2381139A1
Authority
CA
Canada
Prior art keywords
gene
channel
mspa
aforementioned
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002381139A
Other languages
English (en)
Inventor
Michael Niederweis
Stefan Bossmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19943520A external-priority patent/DE19943520A1/de
Application filed by Individual filed Critical Individual
Publication of CA2381139A1 publication Critical patent/CA2381139A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé pour produire une protéine formant un canal, présente dans des bactéries à Gram positif. Selon ce procédé, cette protéine est obtenue par a) surexpression hétérologue ou b) purification de la protéine contenue dans des mycobactéries, la température d'extraction étant supérieure à 50 ·C.
CA002381139A 1999-08-31 2000-08-28 Procede pour produire une proteine formant un canal Abandoned CA2381139A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE19941416 1999-08-31
DE19941416.5 1999-08-31
DE19943520.0 1999-09-11
DE19943520A DE19943520A1 (de) 1999-08-31 1999-09-11 Verfahren zur Herstellung eines kanalbildenden Proteins
PCT/DE2000/002924 WO2001016327A2 (fr) 1999-08-31 2000-08-28 Procede pour produire une proteine formant un canal

Publications (1)

Publication Number Publication Date
CA2381139A1 true CA2381139A1 (fr) 2001-03-08

Family

ID=26054779

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002381139A Abandoned CA2381139A1 (fr) 1999-08-31 2000-08-28 Procede pour produire une proteine formant un canal

Country Status (6)

Country Link
EP (1) EP1208207A2 (fr)
JP (1) JP2003508054A (fr)
AU (1) AU7901300A (fr)
CA (1) CA2381139A1 (fr)
DE (1) DE10082576D2 (fr)
WO (1) WO2001016327A2 (fr)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9751915B2 (en) 2011-02-11 2017-09-05 Oxford Nanopore Technologies Ltd. Mutant pores
US9777049B2 (en) 2012-04-10 2017-10-03 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
US10006905B2 (en) 2013-03-25 2018-06-26 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10167503B2 (en) 2014-05-02 2019-01-01 Oxford Nanopore Technologies Ltd. Mutant pores
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
US10385389B2 (en) 2014-01-22 2019-08-20 Oxford Nanopore Technologies Ltd. Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
US10472673B2 (en) 2015-02-19 2019-11-12 Oxford Nanopore Technologies Ltd. Hetero-pores
US10976300B2 (en) 2015-12-08 2021-04-13 Katholieke Universiteit Leuven Modified nanopores, compositions comprising the same, and uses thereof
US10975428B2 (en) 2016-03-02 2021-04-13 Oxford Nanopore Technologies Ltd. Mutant pore
US11104709B2 (en) 2016-04-06 2021-08-31 Oxford Nanopore Technologies Ltd. Mutant pore
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US11307192B2 (en) 2015-02-19 2022-04-19 Oxford Nanopore Technologies Plc Method for producing a hetero-oligomeric pore comprising two different monomers in a specific stiochiometric ratio
US11572387B2 (en) 2017-06-30 2023-02-07 Vib Vzw Protein pores
US12024541B2 (en) 2017-05-04 2024-07-02 Oxford Nanopore Technologies Plc Transmembrane pore consisting of two CsgG pores
US12173364B2 (en) 2009-03-25 2024-12-24 Oxford University Innovation Limited Method for sequencing a heteropolymeric target nucleic acid sequence

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010034018A2 (fr) 2008-09-22 2010-03-25 University Of Washington Nanopores msp et procédés associés
US10781237B2 (en) 2014-04-16 2020-09-22 The Uab Research Foundation MSP nanopores and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU682538B2 (en) * 1993-11-16 1997-10-09 Becton Dickinson & Company Process for lysing mycobacteria
ID26327A (id) * 1997-12-23 2000-12-14 Genesis Res & Dev Corp Ltd Komposisi-komposisi turunan dari mycobacterium vaccae dan metoda untuk penggunaannya

Cited By (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12173364B2 (en) 2009-03-25 2024-12-24 Oxford University Innovation Limited Method for sequencing a heteropolymeric target nucleic acid sequence
US9751915B2 (en) 2011-02-11 2017-09-05 Oxford Nanopore Technologies Ltd. Mutant pores
US10882889B2 (en) 2012-04-10 2021-01-05 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
US9777049B2 (en) 2012-04-10 2017-10-03 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
US12258375B2 (en) 2012-04-10 2025-03-25 Oxford Nanopore Technologies Plc Mutant lysenin pores
US11845780B2 (en) 2012-04-10 2023-12-19 Oxford Nanopore Technologies Plc Mutant lysenin pores
US11761956B2 (en) 2013-03-25 2023-09-19 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10514378B2 (en) 2013-03-25 2019-12-24 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10802015B2 (en) 2013-03-25 2020-10-13 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10976311B2 (en) 2013-03-25 2021-04-13 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10006905B2 (en) 2013-03-25 2018-06-26 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10385389B2 (en) 2014-01-22 2019-08-20 Oxford Nanopore Technologies Ltd. Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US11725235B2 (en) 2014-01-22 2023-08-15 Oxford Nanopore Technologies Plc Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US10167503B2 (en) 2014-05-02 2019-01-01 Oxford Nanopore Technologies Ltd. Mutant pores
US11739377B2 (en) 2014-05-02 2023-08-29 Oxford Nanopore Technologies Plc Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10844432B2 (en) 2014-05-02 2020-11-24 Oxford Nanopore Technologies, Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US12275761B2 (en) 2014-09-01 2025-04-15 Vib Vzw Mutant CsgG pores
US11034734B2 (en) 2014-09-01 2021-06-15 Oxford Nanopore Technologies Ltd. Mutant pores
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
US11307192B2 (en) 2015-02-19 2022-04-19 Oxford Nanopore Technologies Plc Method for producing a hetero-oligomeric pore comprising two different monomers in a specific stiochiometric ratio
US10472673B2 (en) 2015-02-19 2019-11-12 Oxford Nanopore Technologies Ltd. Hetero-pores
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
US10976300B2 (en) 2015-12-08 2021-04-13 Katholieke Universiteit Leuven Modified nanopores, compositions comprising the same, and uses thereof
US11597970B2 (en) 2016-03-02 2023-03-07 Oxford Nanopore Technologies Plc Mutant pores
US12227800B2 (en) 2016-03-02 2025-02-18 Oxford Nanopore Technologies Plc Mutant pores
US10975428B2 (en) 2016-03-02 2021-04-13 Oxford Nanopore Technologies Ltd. Mutant pore
US11186868B2 (en) 2016-03-02 2021-11-30 Oxford Nanopore Technologies Plc Mutant pore
US11685949B2 (en) 2016-03-02 2023-06-27 Oxford Nanopore Technologies Plc Mutant pore
US10995372B2 (en) 2016-03-02 2021-05-04 Oxford Nanopore Technologies Ltd. Mutant pores
US12018326B2 (en) 2016-03-02 2024-06-25 Oxford Nanopore Technologies Plc Mutant pore
US11939359B2 (en) 2016-04-06 2024-03-26 Oxford Nanopore Technologies Plc Mutant pore
US11104709B2 (en) 2016-04-06 2021-08-31 Oxford Nanopore Technologies Ltd. Mutant pore
US12371458B2 (en) 2016-04-06 2025-07-29 Oxford Nanopore Technologies Plc Mutant pore
US12024541B2 (en) 2017-05-04 2024-07-02 Oxford Nanopore Technologies Plc Transmembrane pore consisting of two CsgG pores
US12084477B2 (en) 2017-06-30 2024-09-10 Vib Vzw Protein pores
US11945840B2 (en) 2017-06-30 2024-04-02 Vib Vzw Protein pores
US11572387B2 (en) 2017-06-30 2023-02-07 Vib Vzw Protein pores

Also Published As

Publication number Publication date
WO2001016327A2 (fr) 2001-03-08
DE10082576D2 (de) 2002-12-19
JP2003508054A (ja) 2003-03-04
EP1208207A2 (fr) 2002-05-29
WO2001016327A3 (fr) 2001-05-31
AU7901300A (en) 2001-03-26

Similar Documents

Publication Publication Date Title
CA2381139A1 (fr) Procede pour produire une proteine formant un canal
US12378604B2 (en) Alpha-hemolysin variants and uses thereof
Rehm et al. Overexpression of algE in Escherichia coli: subcellular localization, purification, and ion channel properties
US11261488B2 (en) Alpha-hemolysin variants
Nagamatsu et al. A toxic fragment from the entomocidal crystal protein of Bacillus thuringiensis
Niederweis et al. Cloning of the mspA gene encoding a porin from Mycobacterium smegmatis
US5965400A (en) DNA encoding a truncated pneumogoccal surface protein (PspA)
Kuwajima Construction of a minimum-size functional flagellin of Escherichia coli
US5571718A (en) Cloning and expression of soluble truncated variants of Borrelia OspA, OspB and Vmp7
IL98031A (en) A method of expanding the range of host proteins or increasing the toxicity of insecticidal proteins, proteins and their preparation
EP0641861B1 (fr) Procédé de production de polypeptides, proteines et peptides hydrophobiques
Beard et al. Detection of a protein, similar to the sex pilus subunit, in the outer membrane of Escherichia coli cells carrying a derepressed F-like R factor
Baty et al. A 136‐amino‐acid‐residue COOH‐terminal fragment of colicin A is endowed with ionophoric activity
WO2000059537A1 (fr) Systeme assurant la secretion efficace des proteines de recombinaison
Lux et al. Overproduced Salmonella typhimurium flagellar motor switch complexes
Schmitt et al. Manipulation of charge distribution in the arginine and glutamate clusters of the OmpG pore alters sugar specificity and ion selectivity
Nagahama et al. Clostridium perfringens β-toxin is sensitive to thiol-group modification but does not require a thiol group for lethal activity
Heinz et al. High-level expression of the mycobacterial porin MspA in Escherichia coli and purification of the recombinant protein
EP0620860A1 (fr) Preparation de proteines borrelia recombinees
Morrison et al. Cloning, expression, purification and properties of a putative multidrug resistance efflux protein from Helicobacter pylori
Kumar et al. Overexpression, refolding, and purification of the major immunodominant outer membrane porin OmpC from Salmonella typhi: characterization of refolded OmpC
Seol et al. Site-directed mutants of Escherichia coli. alpha.-ketoglutarate permease (KgtP)
Fujihira et al. Roles of acidic residues in the hydrophilic loop regions of metal-tetracycline/H+ antiporter Tet (K) of Staphylococcus aureus
JP2609786B2 (ja) 甲虫目昆虫の幼虫に対する殺虫性タンパク質、及びその殺虫性タンパク質をコードする新規ｄｎａ
EP4196608B1 (fr) Nucléosides -5 '-oligophosphates marqués avec des polymères à chargé positive, nanopores incorporant des charges négatives, et procédés et systèmes les utilisant

Legal Events

Date Code Title Description
FZDE Discontinued