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CA2379836A1 - Systeme de cytometrie par balayage, a laser de microvolume - Google Patents

Systeme de cytometrie par balayage, a laser de microvolume Download PDF

Info

Publication number
CA2379836A1
CA2379836A1 CA002379836A CA2379836A CA2379836A1 CA 2379836 A1 CA2379836 A1 CA 2379836A1 CA 002379836 A CA002379836 A CA 002379836A CA 2379836 A CA2379836 A CA 2379836A CA 2379836 A1 CA2379836 A1 CA 2379836A1
Authority
CA
Canada
Prior art keywords
particles
sample
pixel values
light
threshold
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002379836A
Other languages
English (en)
Inventor
Aaron B. Kantor
Louis J. Dietz
Chih-Hua Chung
Scott Norton
Shalom Tsur
Ian Walton
Byron Lee
James L. Winkler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alavita Pharmaceuticals Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2379836A1 publication Critical patent/CA2379836A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Signal Processing (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention porte sur un système intégré amélioré d'identification de marqueurs biologiques recourant à la MLSC (16) (microscopie à balayage à laser (11) de microvolume) pour mesurer les motifs d'expression de marqueurs biologiques dans des fluides (10) biologiques, et à une instrumentation améliorée pour exécuter la MLSC, ainsi qu'à des méthodes améliorées de détection des particules et d'analyse. Ledit système comporte une architecture informatique pour l'analyse des données obtenues par MLSC, en tandem avec celle d'autres informations à caractère médical.
CA002379836A 1999-07-21 2000-04-26 Systeme de cytometrie par balayage, a laser de microvolume Abandoned CA2379836A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14479899P 1999-07-21 1999-07-21
US60/144,798 1999-07-21
PCT/US2000/011133 WO2001008081A1 (fr) 1999-07-21 2000-04-26 Systeme de cytometrie par balayage, a laser de microvolume

Publications (1)

Publication Number Publication Date
CA2379836A1 true CA2379836A1 (fr) 2001-02-01

Family

ID=22510184

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002379836A Abandoned CA2379836A1 (fr) 1999-07-21 2000-04-26 Systeme de cytometrie par balayage, a laser de microvolume

Country Status (8)

Country Link
EP (1) EP1203339A4 (fr)
JP (1) JP2003505707A (fr)
KR (1) KR20020013970A (fr)
AU (1) AU4491100A (fr)
CA (1) CA2379836A1 (fr)
MX (1) MXPA01013398A (fr)
NZ (1) NZ516637A (fr)
WO (1) WO2001008081A1 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7192778B2 (en) 1999-10-06 2007-03-20 Natan Michael J Surface enhanced spectroscopy-active composite nanoparticles
US8497131B2 (en) 1999-10-06 2013-07-30 Becton, Dickinson And Company Surface enhanced spectroscopy-active composite nanoparticles comprising Raman-active reporter molecules
WO2002079764A1 (fr) 2001-01-26 2002-10-10 Nanoplex Technologies, Inc. Nanoparticules sandwich a spectrometrie active exaltees de surface
WO2002025280A1 (fr) 2000-09-20 2002-03-28 Surromed, Inc. Procede de surveillance de plaquettes au repos et activees dans des echantillons sanguins non fixes
US7572642B2 (en) 2001-04-18 2009-08-11 Ambrigen, Llc Assay based on particles, which specifically bind with targets in spatially distributed characteristic patterns
DE10291983D2 (de) * 2001-05-07 2004-04-15 Deutsches Krebsforsch Fluoreszenzfluktuationsmikroskop, -messmodul bzw.-scanmodul und Verfahren zur Fluoreszenzfluktuationsmessung sowie Verfahren und Vorrichtung zur Justage eines Fluoreszenzfluktuationsmikroskops
EP1889032A1 (fr) * 2005-05-25 2008-02-20 Stiftelsen Universitetsforskning Bergen Systeme de microscope et procede de criblage de medicaments, physiotherapies et biorisques
US8409863B2 (en) 2005-12-14 2013-04-02 Becton, Dickinson And Company Nanoparticulate chemical sensors using SERS
US7796251B2 (en) * 2006-03-22 2010-09-14 Itt Manufacturing Enterprises, Inc. Method, apparatus and system for rapid and sensitive standoff detection of surface contaminants
JP4909254B2 (ja) * 2007-12-26 2012-04-04 日本電信電話株式会社 浮遊粒子状物質測定装置
EP2425226B1 (fr) * 2009-04-27 2020-10-07 El Spectra, LLC Instrument à pipette
CN110031384B (zh) * 2012-05-30 2023-03-10 艾瑞斯国际有限公司 流式细胞仪
US9746412B2 (en) 2012-05-30 2017-08-29 Iris International, Inc. Flow cytometer
JP7201297B2 (ja) * 2018-09-26 2023-01-10 シスメックス株式会社 フローサイトメーター、データ送信方法及び情報処理システム
JP2022516556A (ja) * 2019-01-03 2022-02-28 ピクセル メディカル テクノロジーズ リミテッド 流体サンプルを分析するシステム及び方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE455646B (sv) * 1984-10-22 1988-07-25 Radians Innova Ab Fluorescensanordning
US5107422A (en) * 1989-10-02 1992-04-21 Kamentsky Louis A Method and apparatus for measuring multiple optical properties of biological specimens
US5127730A (en) * 1990-08-10 1992-07-07 Regents Of The University Of Minnesota Multi-color laser scanning confocal imaging system
JPH0599912A (ja) * 1991-10-11 1993-04-23 Union Shokai:Kk アナログフイルター装置
CA2130340C (fr) * 1992-02-18 2000-06-06 Shih-Jong James Lee Methode de reconnaissance d'objets par traitements de donnees
US5377003A (en) * 1992-03-06 1994-12-27 The United States Of America As Represented By The Department Of Health And Human Services Spectroscopic imaging device employing imaging quality spectral filters
US5889881A (en) * 1992-10-14 1999-03-30 Oncometrics Imaging Corp. Method and apparatus for automatically detecting malignancy-associated changes
US5556764A (en) * 1993-02-17 1996-09-17 Biometric Imaging, Inc. Method and apparatus for cell counting and cell classification
US5456252A (en) * 1993-09-30 1995-10-10 Cedars-Sinai Medical Center Induced fluorescence spectroscopy blood perfusion and pH monitor and method
JP3474612B2 (ja) * 1993-12-03 2003-12-08 浜松ホトニクス株式会社 光検出装置
JPH08313348A (ja) * 1995-05-16 1996-11-29 Kanagawa Kagaku Gijutsu Akad 赤外光時間応答測定装置
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system

Also Published As

Publication number Publication date
EP1203339A1 (fr) 2002-05-08
KR20020013970A (ko) 2002-02-21
MXPA01013398A (es) 2003-03-27
WO2001008081A1 (fr) 2001-02-01
AU4491100A (en) 2001-02-13
JP2003505707A (ja) 2003-02-12
NZ516637A (en) 2003-08-29
EP1203339A4 (fr) 2006-09-13

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Legal Events

Date Code Title Description
FZDE Discontinued