CA2375098A1 - Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires - Google Patents
Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires Download PDFInfo
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- CA2375098A1 CA2375098A1 CA002375098A CA2375098A CA2375098A1 CA 2375098 A1 CA2375098 A1 CA 2375098A1 CA 002375098 A CA002375098 A CA 002375098A CA 2375098 A CA2375098 A CA 2375098A CA 2375098 A1 CA2375098 A1 CA 2375098A1
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2710/10011—Adenoviridae
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Abstract
L'invention concerne une méthode de fabrication de virus recombinés nécessitant des virus auxiliaires pour l'encapsidation. La méthode fait intervenir un virus auxiliaire recombiné qui a été conçu pour pouvoir contenir au moins un site de restriction rare (par exemple, I-SceI), un vecteur viral qui dépend de l'auxiliaire pour l'encapsidation, et une cellule hôte recombinée pouvant exprimer un enzyme de restriction rare (par exemple, I-SceI). La méthode consiste à transfecter ou à infecter la cellule hôte avec le virus auxiliaire et le vecteur viral et à incuber la cellule dans des conditions qui permettent l'encapsidation du vecteur viral dans une capside d'adénovirus. Ensuite, le I-SceI exprimé par la cellule hôte digère le virus auxiliaire, ce qui permet une séparation immédiate des fragments digérés du virus auxiliaire d'avec le virus recombiné.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13710599P | 1999-06-02 | 1999-06-02 | |
| US60/137,105 | 1999-06-02 | ||
| PCT/US2000/000415 WO2000075353A1 (fr) | 1999-06-02 | 2000-01-07 | Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2375098A1 true CA2375098A1 (fr) | 2000-12-14 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002375098A Abandoned CA2375098A1 (fr) | 1999-06-02 | 2000-01-07 | Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1183380A1 (fr) |
| JP (1) | JP2003501067A (fr) |
| AU (1) | AU2409200A (fr) |
| CA (1) | CA2375098A1 (fr) |
| WO (1) | WO2000075353A1 (fr) |
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| US6924128B2 (en) | 1994-12-06 | 2005-08-02 | Targeted Genetics Corporation | Packaging cell lines for generation of high titers of recombinant AAV vectors |
| US6989264B2 (en) | 1997-09-05 | 2006-01-24 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
| US6346415B1 (en) | 1997-10-21 | 2002-02-12 | Targeted Genetics Corporation | Transcriptionally-activated AAV inverted terminal repeats (ITRS) for use with recombinant AAV vectors |
| US6759237B1 (en) | 1998-11-05 | 2004-07-06 | The Trustees Of The University Of Pennsylvania | Adeno-associated virus serotype 1 nucleic acid sequences, vectors and host cells containing same |
| US6893865B1 (en) | 1999-04-28 | 2005-05-17 | Targeted Genetics Corporation | Methods, compositions, and cells for encapsidating recombinant vectors in AAV particles |
| US6951715B2 (en) | 2000-10-30 | 2005-10-04 | Sru Biosystems, Inc. | Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements |
| US7153702B2 (en) | 2000-10-30 | 2006-12-26 | Sru Biosystems, Inc. | Label-free methods for performing assays using a colorimetric resonant reflectance optical biosensor |
| US7217574B2 (en) | 2000-10-30 | 2007-05-15 | Sru Biosystems, Inc. | Method and apparatus for biosensor spectral shift detection |
| US7023544B2 (en) | 2000-10-30 | 2006-04-04 | Sru Biosystems, Inc. | Method and instrument for detecting biomolecular interactions |
| US7094595B2 (en) | 2000-10-30 | 2006-08-22 | Sru Biosystems, Inc. | Label-free high-throughput optical technique for detecting biomolecular interactions |
| US7175980B2 (en) | 2000-10-30 | 2007-02-13 | Sru Biosystems, Inc. | Method of making a plastic colorimetric resonant biosensor device with liquid handling capabilities |
| US7202076B2 (en) | 2000-10-30 | 2007-04-10 | Sru Biosystems, Inc. | Label-free high-throughput optical technique for detecting biomolecular interactions |
| US7306827B2 (en) | 2000-10-30 | 2007-12-11 | Sru Biosystems, Inc. | Method and machine for replicating holographic gratings on a substrate |
| US7101660B2 (en) | 2000-10-30 | 2006-09-05 | Sru Biosystems, Inc. | Method for producing a colorimetric resonant reflection biosensor on rigid surfaces |
| US7264973B2 (en) | 2000-10-30 | 2007-09-04 | Sru Biosystems, Inc. | Label-free methods for performing assays using a colorimetric resonant optical biosensor |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5792632A (en) * | 1992-05-05 | 1998-08-11 | Institut Pasteur | Nucleotide sequence encoding the enzyme I-SceI and the uses thereof |
| CA2592997A1 (fr) * | 1992-12-03 | 1994-06-09 | Genzyme Corporation | Vecteurs pseudo-adenoviraux |
| EP0694070B1 (fr) * | 1993-09-15 | 2002-04-10 | Chiron Corporation | Vecteurs composes d'alphavirus recombinants |
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- 2000-01-07 CA CA002375098A patent/CA2375098A1/fr not_active Abandoned
- 2000-01-07 WO PCT/US2000/000415 patent/WO2000075353A1/fr not_active Ceased
- 2000-01-07 AU AU24092/00A patent/AU2409200A/en not_active Abandoned
- 2000-01-07 EP EP00902362A patent/EP1183380A1/fr not_active Withdrawn
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| JP2003501067A (ja) | 2003-01-14 |
| EP1183380A1 (fr) | 2002-03-06 |
| WO2000075353A1 (fr) | 2000-12-14 |
| AU2409200A (en) | 2000-12-28 |
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