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CA2370656A1 - Kit d'extraction d'adn magnetique pour plantes - Google Patents

Kit d'extraction d'adn magnetique pour plantes Download PDF

Info

Publication number
CA2370656A1
CA2370656A1 CA002370656A CA2370656A CA2370656A1 CA 2370656 A1 CA2370656 A1 CA 2370656A1 CA 002370656 A CA002370656 A CA 002370656A CA 2370656 A CA2370656 A CA 2370656A CA 2370656 A1 CA2370656 A1 CA 2370656A1
Authority
CA
Canada
Prior art keywords
dna
plant material
kit
lysis buffer
positively charged
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002370656A
Other languages
English (en)
Inventor
Mitchell T. Gore
Maria-Luisa Maccecchini
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Annovis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Annovis Inc filed Critical Annovis Inc
Publication of CA2370656A1 publication Critical patent/CA2370656A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention concerne un procédé et un kit pour l'extraction d'ADN de plantes, permettant de produire rapidement de l'ADN de plantes présentant un grand degré de pureté. Ledit procédé permet d'isoler de l'ADN (ADN génomique, de chloroplaste et/ou mitochondrien) au moyen de groupes anioniques immobilisés, de préférence sur un substrat chromatographique ou idéalement sur des billes magnétiques dérivées de groupes anioniques, tels que le diéthylaminoéthyle (DEAE), par une interaction à échange d'anions. L'ADN purifié est ensuite élué à l'aide d'ions (généralement une solution salée). L'ARN peut être supprimé par digestion à l'aide de Rnase.
CA002370656A 1999-04-21 2000-04-21 Kit d'extraction d'adn magnetique pour plantes Abandoned CA2370656A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13035399P 1999-04-21 1999-04-21
US60/130,353 1999-04-21
PCT/US2000/010834 WO2000063362A1 (fr) 1999-04-21 2000-04-21 Kit d'extraction d'adn magnetique pour plantes

Publications (1)

Publication Number Publication Date
CA2370656A1 true CA2370656A1 (fr) 2000-10-26

Family

ID=22444292

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002370656A Abandoned CA2370656A1 (fr) 1999-04-21 2000-04-21 Kit d'extraction d'adn magnetique pour plantes

Country Status (5)

Country Link
EP (1) EP1171584A1 (fr)
JP (1) JP2002541839A (fr)
AU (1) AU4367500A (fr)
CA (1) CA2370656A1 (fr)
WO (1) WO2000063362A1 (fr)

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US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US20040121311A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in livestock
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
DE60234464D1 (de) 2001-11-28 2009-12-31 Applied Biosystems Llc Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung
EP1578399A4 (fr) 2002-12-06 2007-11-28 Isis Pharmaceuticals Inc Procedes d'identification rapide de pathogenes chez l'homme et les betes
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) * 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
US8242254B2 (en) 2003-09-11 2012-08-14 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
JP4810533B2 (ja) 2004-05-24 2011-11-09 アイビス バイオサイエンシズ インコーポレイティッド ディジタルスレショルド化による選択的イオン濾過作用を用いた質量分光測定法
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (fr) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Procedes pour l'identification rapide d'organismes recombinants
CA2579804C (fr) 2004-09-16 2013-12-10 Cropdesign N.V. Evaluation de la croissance d'une racine
KR100601972B1 (ko) 2004-11-03 2006-07-18 삼성전자주식회사 레이저와 비드를 이용한 상 분리에 의한 핵산의 정제 장치및 방법
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US8182992B2 (en) 2005-03-03 2012-05-22 Ibis Biosciences, Inc. Compositions for use in identification of adventitious viruses
WO2007014045A2 (fr) 2005-07-21 2007-02-01 Isis Pharmaceuticals, Inc. Procede pour l'identification et la quantification rapide de variants d'acide nucleique
WO2008143627A2 (fr) 2006-09-14 2008-11-27 Ibis Biosciences, Inc. Procédé d'amplification ciblée de génome entier pour l'identification d'agents pathogènes
JP5680304B2 (ja) 2007-02-23 2015-03-04 アイビス バイオサイエンシズ インコーポレイティッド 迅速な法医学的ｄｎａ分析法
WO2008151023A2 (fr) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Procédés et compositions pour l'amplification par déplacement multiple d'acides nucléiques
EP2349549B1 (fr) 2008-09-16 2012-07-18 Ibis Biosciences, Inc. Cartouches de mélange, postes de mélange et kits, et système associé
CN105638027B (zh) * 2008-09-16 2019-09-27 巴斯夫植物科学有限公司 改善的植物育种方法
EP2347254A2 (fr) 2008-09-16 2011-07-27 Ibis Biosciences, Inc. Unités de traitement d'échantillons, systèmes et procédés associés
WO2010033625A1 (fr) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Systèmes de manipulation de microplaques et produits-programmes informatiques et procédés connexes
WO2010093943A1 (fr) 2009-02-12 2010-08-19 Ibis Biosciences, Inc. Ensembles sonde d'ionisation
WO2011008971A1 (fr) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Appareil de levage et de montage
WO2011008972A1 (fr) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Systèmes pour l'identification d'un bioagent
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
CN102533731A (zh) * 2012-01-19 2012-07-04 西北农林科技大学 一种番茄基因组总dna提取试剂盒及其提取方法
CN102618532A (zh) * 2012-05-02 2012-08-01 易春 基于磁珠法从植物叶片中提取基因组dna的试剂盒及其提取方法
CN102888397A (zh) * 2012-09-25 2013-01-23 杭州硕航生物科技有限公司 一种利用磁珠提取全血基因组dna的试剂盒及应用
CN110452905B (zh) * 2019-08-22 2023-06-02 云南省烟草农业科学研究院 一种提高烟草dna沉淀效率的提取方法及其应用
CN110669759A (zh) * 2019-10-28 2020-01-10 北京百迈客生物科技有限公司 一种适用于纳米孔测序的真菌高纯度长片段基因组dna提取方法
CN112921028A (zh) * 2019-12-06 2021-06-08 深圳华大基因科技服务有限公司 Dna纯化方法、基因组dna的提取方法、测序方法和试剂盒
CN115856285B (zh) * 2022-12-27 2025-06-24 太原理工大学 一种绿色制备免表面修饰的磁性粒子增强型生物分子检测方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5981235A (en) * 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
ATE386044T1 (de) * 1997-12-06 2008-03-15 Invitrogen Corp Isolierung von nukleinsäuren

Also Published As

Publication number Publication date
WO2000063362A1 (fr) 2000-10-26
JP2002541839A (ja) 2002-12-10
AU4367500A (en) 2000-11-02
EP1171584A1 (fr) 2002-01-16

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued