[go: up one dir, main page]

CA2365226A1 - Immobilisation de polynucleotides - Google Patents

Immobilisation de polynucleotides Download PDF

Info

Publication number
CA2365226A1
CA2365226A1 CA002365226A CA2365226A CA2365226A1 CA 2365226 A1 CA2365226 A1 CA 2365226A1 CA 002365226 A CA002365226 A CA 002365226A CA 2365226 A CA2365226 A CA 2365226A CA 2365226 A1 CA2365226 A1 CA 2365226A1
Authority
CA
Canada
Prior art keywords
polynucleotide
nucleotide
polynucleotides
solid support
support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002365226A
Other languages
English (en)
Inventor
Angelika Josefine Rosler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare UK Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2365226A1 publication Critical patent/CA2365226A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention concerne une méthode servant à immobiliser un nucléotide ou un polynucléotide sur un support solide, consistant à : fournir un support solide présentant une surface fonctionnalisée de formule B - S - S L, dans laquelle B est le support solide et L est un groupe partant ; fournir un nucléotide ou un polynucléotide comprenant au moins un groupe phosphorothioate ; et coupler les deux ensemble grâce à une réaction d'échange sulfurée. Le produit présente la structure B - S - S - P(O)R?1¿R?2¿, dans laquelle - POR?1¿R?2¿ représente un nucléotide ou un polynucléotide. Les ensembles de polynucléotides immobilisés sont utiles pour la mise en oeuvre de techniques APEX ou SBH.
CA002365226A 1999-03-09 2000-03-07 Immobilisation de polynucleotides Abandoned CA2365226A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP99301764 1999-03-09
EP99301764.9 1999-03-09
PCT/GB2000/000843 WO2000053616A2 (fr) 1999-03-09 2000-03-07 Immobilisation de polynucleotides

Publications (1)

Publication Number Publication Date
CA2365226A1 true CA2365226A1 (fr) 2000-09-14

Family

ID=8241258

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002365226A Abandoned CA2365226A1 (fr) 1999-03-09 2000-03-07 Immobilisation de polynucleotides

Country Status (5)

Country Link
EP (1) EP1259524A2 (fr)
JP (1) JP2003520766A (fr)
AU (1) AU2930800A (fr)
CA (1) CA2365226A1 (fr)
WO (1) WO2000053616A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030143331A1 (en) * 2001-10-03 2003-07-31 Micrologix Biotech Inc. Attachment of thiophosphate tethered oligonucleotides to a solid surface
DE10159904A1 (de) * 2001-12-06 2003-07-03 Adnagen Ag Oligonukleotidanordnung, Verfahren zum Nukleotidnachweis sowie Vorrichtung hierfür
GB0326073D0 (en) 2003-11-07 2003-12-10 Solexa Ltd Improvements in or relating to polynucleotide arrays
US8067164B2 (en) 2007-08-12 2011-11-29 Integrated Dna Technologies, Inc. Microarray system with improved sequence specificity

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990001564A1 (fr) * 1988-08-09 1990-02-22 Microprobe Corporation Procedes d'analyse a cibles multiples par hybridation d'acides nucleiques
US5837860A (en) * 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds

Also Published As

Publication number Publication date
EP1259524A2 (fr) 2002-11-27
JP2003520766A (ja) 2003-07-08
WO2000053616A3 (fr) 2002-09-12
WO2000053616A2 (fr) 2000-09-14
AU2930800A (en) 2000-09-28

Similar Documents

Publication Publication Date Title
EP1692152B1 (fr) Ameliorations apportees a des reseaux polynuceotidiques
US5919626A (en) Attachment of unmodified nucleic acids to silanized solid phase surfaces
US7033760B2 (en) Metallic solid supports modified with nucleic acids
CA2252911A1 (fr) Fixation covalente de molecules d'acide nucleique sur des phases solides au moyen de ponts disulfures
US6893822B2 (en) Enzymatic modification of a nucleic acid-synthetic binding unit conjugate
CA2477503A1 (fr) Modification de surface, attache de lieur, et procedes de polymerisation
US9266726B2 (en) Method for making biochips
US7049073B2 (en) Double stranded nucleic acid biochips
WO2005021725A2 (fr) Methodes d'analyse de l'acide nucleique effectuees dans de petits volumes de reaction
CA2365226A1 (fr) Immobilisation de polynucleotides
CA2381761A1 (fr) Procede d'ancrage d'oligonucleotides a un substrat
JPH08238100A (ja) 所定の配列を有する核酸を特異的に検出する方法、並びに該方法に使用されるプローブ及びキット
JPWO2007069608A1 (ja) プローブ固定化用担体の製造方法、ｄｎａアレイの製造方法及びプローブ固定化用担体
NZ536909A (en) Target nucleic acid conjugating system comprising a solid support having a tag oligonucleotide covalently bound to the surface of the solid support
Chernov et al. Double stranded nucleic acid biochips
AU2003229125B2 (en) A nucleic acid anchoring system comprising covalent linkage of an oligonucleotide to a solid support
EP1660515A2 (fr) Methodes d'analyse de l'acide nucleique effectuees dans de petits volumes de reaction

Legal Events

Date Code Title Description
FZDE Discontinued