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CA2357011A1 - Procede multiplex de detection d'acides nucleiques - Google Patents

Procede multiplex de detection d'acides nucleiques Download PDF

Info

Publication number
CA2357011A1
CA2357011A1 CA002357011A CA2357011A CA2357011A1 CA 2357011 A1 CA2357011 A1 CA 2357011A1 CA 002357011 A CA002357011 A CA 002357011A CA 2357011 A CA2357011 A CA 2357011A CA 2357011 A1 CA2357011 A1 CA 2357011A1
Authority
CA
Canada
Prior art keywords
nucleic acid
seq
probe
probes
nucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002357011A
Other languages
English (en)
Inventor
Martin K. Lewis
Daniel Kephart
Richard B. Rhodes
John W. Shultz
Donna Leippe
Michelle Mandrekar
Christine Ann Andrews
James R. Hartnett
Trent Gu
Keith V. Wood
Roy Welch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/252,436 external-priority patent/US6159693A/en
Priority claimed from PCT/US1999/005304 external-priority patent/WO1999046409A1/fr
Priority claimed from US09/358,972 external-priority patent/US6235480B1/en
Priority claimed from US09/406,064 external-priority patent/US6270973B1/en
Application filed by Individual filed Critical Individual
Publication of CA2357011A1 publication Critical patent/CA2357011A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des procédés utilisant la dépolymérisation d'un hybride d'acide nucléique pour analyser qualitativement et quantitativement la présence de séquences cibles d'acides nucléiques prédéterminées au moyen d'une méthode de dosage multiplex. Les applications de ces procédés comprennent la détection de polymorphismes de nucléotides simples, l'identification de changements de bases simples, la spéciation, le génotypage, les diagnostics de marqueurs médicaux, et les applications associées.
CA002357011A 1999-02-18 2000-02-18 Procede multiplex de detection d'acides nucleiques Abandoned CA2357011A1 (fr)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US09/252,436 1999-02-18
US09/252,436 US6159693A (en) 1998-03-13 1999-02-18 Nucleic acid detection
PCT/US1999/005304 WO1999046409A1 (fr) 1998-03-13 1999-03-11 Detection d'acide nucleique
USPCT/US99/05304 1999-03-11
US09/358,972 1999-07-21
US09/358,972 US6235480B1 (en) 1998-03-13 1999-07-21 Detection of nucleic acid hybrids
US09/406,064 1999-09-27
US09/406,064 US6270973B1 (en) 1998-03-13 1999-09-27 Multiplex method for nucleic acid detection
PCT/US2000/004243 WO2000049181A1 (fr) 1999-02-18 2000-02-18 Procede multiplex de detection d'acides nucleiques

Publications (1)

Publication Number Publication Date
CA2357011A1 true CA2357011A1 (fr) 2000-08-24

Family

ID=27400552

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002357011A Abandoned CA2357011A1 (fr) 1999-02-18 2000-02-18 Procede multiplex de detection d'acides nucleiques

Country Status (5)

Country Link
EP (1) EP1155150A1 (fr)
JP (1) JP2003535568A (fr)
AU (1) AU774085B2 (fr)
CA (1) CA2357011A1 (fr)
WO (1) WO2000049181A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
WO2002063049A2 (fr) * 2001-02-02 2002-08-15 Genome Therapeutics Corporation Methodes de determination d'un nucleotide a un emplacement specifique dans une molecule d'acide nucleique
GB0111275D0 (en) * 2001-05-09 2001-06-27 Secr Defence Analytical method and kit
EP1550717B1 (fr) * 2002-05-31 2010-09-08 Nippon Steel Kankyo Engineering Co., Ltd. Nouveau procede de dosage d'acide nucleique au moyen d'un nucleotide marque
WO2014093291A1 (fr) * 2012-12-10 2014-06-19 Advandx, Inc. Utilisation de sondes pour l'identification par spectrométrie de masse de micro-organismes ou de cellules et de pathologies associées présentant un intérêt

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4735897A (en) * 1985-05-02 1988-04-05 Allied Corporation Method and kit for detecting polyriboadenosine segments and messenger RNA
US5004803A (en) * 1988-11-14 1991-04-02 Genetics Institute, Inc. Production of procoagulant proteins
AU5640090A (en) * 1989-03-21 1990-11-05 Collaborative Research Inc. A dna diagnostic test using an exonuclease activity
EP0663447B1 (fr) * 1993-12-28 2003-07-09 Eiken Chemical Co., Ltd. Procédé pour la détection de polynucléotides spécifiques
DK0807691T3 (da) * 1994-02-14 2002-02-04 Univ Leiden Fremgangsmåde til screening for tilstedeværelse af en genetisk defekt forbundet med trombose og/eller lav antikoagulant reaktion på aktiveret protein C
WO1996021144A1 (fr) * 1994-12-30 1996-07-11 Georgetown University Methode de detection fluorometrique de clivage d'acide nucleique
US5945284A (en) * 1997-05-27 1999-08-31 The Perkin-Elmer Corporation Length determination of nucleic acid repeat sequences by discontinuous primer extension
US6335162B1 (en) * 1998-03-13 2002-01-01 Promega Corporation Nucleic acid detection

Also Published As

Publication number Publication date
WO2000049181A1 (fr) 2000-08-24
EP1155150A1 (fr) 2001-11-21
JP2003535568A (ja) 2003-12-02
AU774085B2 (en) 2004-06-17
AU3001900A (en) 2000-09-04

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