CA2354030A1 - Genes de micromonospora echinospora codant pour la biosynthese de calicheamicine et auto-resistance a cette derniere - Google Patents
Genes de micromonospora echinospora codant pour la biosynthese de calicheamicine et auto-resistance a cette derniere Download PDFInfo
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- CA2354030A1 CA2354030A1 CA002354030A CA2354030A CA2354030A1 CA 2354030 A1 CA2354030 A1 CA 2354030A1 CA 002354030 A CA002354030 A CA 002354030A CA 2354030 A CA2354030 A CA 2354030A CA 2354030 A1 CA2354030 A1 CA 2354030A1
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- nucleic acid
- calicheamicin
- acid molecule
- gene
- isolated nucleic
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
- C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Cette invention concerne un groupe de gènes isolés de Micromonospora echinospora qui code pour la biosynthèse de calichéamicine. Ce groupe de gènes biosynthétique contient des gènes codant pour les protéines et les enzymes que l'on utilise dans la production biosynthétique de calichéamicine, y compris un aryltetrasaccharide et un aglycone. Ce groupe de gènes comprend également un gène conférant une résistance à la calichéamicine. Cette invention concerne également des gènes isolés de ce groupe biosynthétique ainsi que leurs protéines correspondantes. Cette invention concerne en outre un ADN qui s'hybride au groupe de gènes de calichéamicine ainsi qu'aux gènes isolés de ce groupe. Cette invention concerne aussi des vecteurs d'expression contenant des gènes du groupe biosynthétique, ainsi que leurs variantes fonctionnelles. Cette invention concerne enfin des cellules hôtes qui sont conjuguées à l'ADN isolé à partir du génome de Micromonospora echinospora spp. calichensis.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11132598P | 1998-12-07 | 1998-12-07 | |
| US60/111,325 | 1998-12-07 | ||
| PCT/US1999/029110 WO2000037608A2 (fr) | 1998-12-07 | 1999-12-07 | Genes de $i(micromonospora echinospora) codant pour la biosynthese de calicheamicine et auto-resistance a cette derniere |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2354030A1 true CA2354030A1 (fr) | 2000-06-29 |
Family
ID=22337859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002354030A Abandoned CA2354030A1 (fr) | 1998-12-07 | 1999-12-07 | Genes de micromonospora echinospora codant pour la biosynthese de calicheamicine et auto-resistance a cette derniere |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1137796A4 (fr) |
| JP (1) | JP2002533067A (fr) |
| CA (1) | CA2354030A1 (fr) |
| WO (1) | WO2000037608A2 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6733998B1 (en) | 1998-12-07 | 2004-05-11 | Sloan-Kettering Institute For Cancer Research | Micromonospora echinospora genes coding for biosynthesis of calicheamicin and self-resistance thereto |
| US7257562B2 (en) | 2000-10-13 | 2007-08-14 | Thallion Pharmaceuticals Inc. | High throughput method for discovery of gene clusters |
| WO2002079465A2 (fr) * | 2000-11-28 | 2002-10-10 | Sloan-Kettering Institute For Cancer Research | Codage de genes de micromonospora echinospora pour la biosynthese de la calicheamicine, et autoresistance vis-a-vis de cette substance |
| CA2387401C (fr) | 2001-05-21 | 2004-10-12 | Ecopia Biosciences Inc. | Compositions, methodes et systemes pour la production d'enediynes |
| SI2029750T1 (sl) * | 2006-06-22 | 2011-11-30 | Dsm Ip Assets Bv | Proizvajanje pravastatina |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5276159A (en) * | 1990-08-01 | 1994-01-04 | The Scripps Research Institute | Dynemicin analogs: syntheses, methods of preparation and use |
| US5712146A (en) * | 1993-09-20 | 1998-01-27 | The Leland Stanford Junior University | Recombinant combinatorial genetic library for the production of novel polyketides |
-
1999
- 1999-12-07 CA CA002354030A patent/CA2354030A1/fr not_active Abandoned
- 1999-12-07 JP JP2000589664A patent/JP2002533067A/ja active Pending
- 1999-12-07 WO PCT/US1999/029110 patent/WO2000037608A2/fr not_active Ceased
- 1999-12-07 EP EP99972435A patent/EP1137796A4/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000037608A2 (fr) | 2000-06-29 |
| EP1137796A2 (fr) | 2001-10-04 |
| EP1137796A4 (fr) | 2005-05-25 |
| WO2000037608A3 (fr) | 2000-11-23 |
| JP2002533067A (ja) | 2002-10-08 |
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