CA2353920A1 - Systeme de test pour reconnaitre differents marqueurs, sa production et son utilisation - Google Patents
Systeme de test pour reconnaitre differents marqueurs, sa production et son utilisation Download PDFInfo
- Publication number
- CA2353920A1 CA2353920A1 CA002353920A CA2353920A CA2353920A1 CA 2353920 A1 CA2353920 A1 CA 2353920A1 CA 002353920 A CA002353920 A CA 002353920A CA 2353920 A CA2353920 A CA 2353920A CA 2353920 A1 CA2353920 A1 CA 2353920A1
- Authority
- CA
- Canada
- Prior art keywords
- natural
- nucleic acid
- substance
- recognition species
- marker
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 29
- 238000004519 manufacturing process Methods 0.000 title claims 2
- 238000001514 detection method Methods 0.000 claims abstract description 62
- 238000000034 method Methods 0.000 claims abstract description 47
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 7
- 241000894007 species Species 0.000 claims description 107
- 239000005445 natural material Substances 0.000 claims description 81
- 150000007523 nucleic acids Chemical class 0.000 claims description 78
- 108020004707 nucleic acids Proteins 0.000 claims description 77
- 102000039446 nucleic acids Human genes 0.000 claims description 77
- 239000003550 marker Substances 0.000 claims description 67
- 239000000126 substance Substances 0.000 claims description 51
- 239000000427 antigen Substances 0.000 claims description 13
- 102000036639 antigens Human genes 0.000 claims description 13
- 108091007433 antigens Proteins 0.000 claims description 13
- 239000000470 constituent Substances 0.000 claims description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 8
- 201000010099 disease Diseases 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 230000002285 radioactive effect Effects 0.000 claims description 6
- 238000010791 quenching Methods 0.000 claims description 5
- 230000000171 quenching effect Effects 0.000 claims description 5
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 claims description 4
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 claims description 4
- 239000012491 analyte Substances 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 claims description 4
- 230000003993 interaction Effects 0.000 claims description 4
- 239000003446 ligand Substances 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 238000012546 transfer Methods 0.000 claims description 4
- 241000700605 Viruses Species 0.000 claims description 3
- 230000002255 enzymatic effect Effects 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 244000052769 pathogen Species 0.000 claims description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 2
- 102000003886 Glycoproteins Human genes 0.000 claims description 2
- 108090000288 Glycoproteins Proteins 0.000 claims description 2
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 claims description 2
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 claims description 2
- 108090001090 Lectins Proteins 0.000 claims description 2
- 102000004856 Lectins Human genes 0.000 claims description 2
- 108010043958 Peptoids Proteins 0.000 claims description 2
- 101710172711 Structural protein Proteins 0.000 claims description 2
- 238000005411 Van der Waals force Methods 0.000 claims description 2
- 241000726445 Viroids Species 0.000 claims description 2
- 239000013566 allergen Substances 0.000 claims description 2
- 150000001720 carbohydrates Chemical class 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 239000000919 ceramic Substances 0.000 claims description 2
- 238000003891 environmental analysis Methods 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 229910052739 hydrogen Inorganic materials 0.000 claims description 2
- 239000001257 hydrogen Substances 0.000 claims description 2
- 230000002209 hydrophobic effect Effects 0.000 claims description 2
- 239000002523 lectin Substances 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 239000002502 liposome Substances 0.000 claims description 2
- 210000004698 lymphocyte Anatomy 0.000 claims description 2
- 239000003226 mitogen Substances 0.000 claims description 2
- 229910000510 noble metal Inorganic materials 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 229930000044 secondary metabolite Natural products 0.000 claims description 2
- 239000003053 toxin Substances 0.000 claims description 2
- 231100000765 toxin Toxicity 0.000 claims description 2
- 108700012359 toxins Proteins 0.000 claims description 2
- 210000000234 capsid Anatomy 0.000 claims 1
- 239000002178 crystalline material Substances 0.000 claims 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 3
- 108020004414 DNA Proteins 0.000 description 21
- 108091034117 Oligonucleotide Proteins 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 108010090804 Streptavidin Proteins 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 238000002821 scintillation proximity assay Methods 0.000 description 6
- 108091023037 Aptamer Proteins 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 241000283707 Capra Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 108091008102 DNA aptamers Proteins 0.000 description 2
- 108091008103 RNA aptamers Proteins 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000010414 supernatant solution Substances 0.000 description 2
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012875 competitive assay Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- -1 proteins Chemical class 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
L'invention concerne un système de test contenant au moins deux espèces de reconnaissance qui reconnaissent au moins deux marqueurs différents, en formant un complexe. L'invention concerne également la production de ce système de test et son utilisation dans un procédé de dépistage approprié.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19859912.9 | 1998-12-23 | ||
| DE19859912A DE19859912C2 (de) | 1998-12-23 | 1998-12-23 | Testsystem zur Erkennung verschiedener Marker, seine Herstellung sowie Verwendung |
| PCT/EP1999/010333 WO2000039581A2 (fr) | 1998-12-23 | 1999-12-22 | Systeme de test pour reconnaitre differents marqueurs, sa production et son utilisation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2353920A1 true CA2353920A1 (fr) | 2000-07-06 |
Family
ID=7892569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002353920A Abandoned CA2353920A1 (fr) | 1998-12-23 | 1999-12-22 | Systeme de test pour reconnaitre differents marqueurs, sa production et son utilisation |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1141725A2 (fr) |
| JP (1) | JP2002533724A (fr) |
| AU (1) | AU773046B2 (fr) |
| CA (1) | CA2353920A1 (fr) |
| DE (1) | DE19859912C2 (fr) |
| WO (1) | WO2000039581A2 (fr) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10018626B2 (en) | 2012-03-09 | 2018-07-10 | Invisible Sentinel, Inc. | Methods and compositions for detecting multiple analytes with a single signal |
| US10495638B2 (en) | 2009-10-09 | 2019-12-03 | Invisible Sentinel, Inc. | Device for detection of analytes and uses thereof |
| WO2020079638A1 (fr) * | 2018-10-17 | 2020-04-23 | Molecular Devices (Austria) GmbH | Dosages de buvardage de western en temps réel utilisant un transfert d'énergie par résonance de fluorescence (fret) |
| US10705084B2 (en) | 2009-07-31 | 2020-07-07 | Invisible Sentinel, Inc. | Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6673562B2 (en) * | 2000-08-24 | 2004-01-06 | Spectral Diagnostics, Inc. | Differential immunoassay |
| EP1947459A3 (fr) * | 2000-08-24 | 2008-09-24 | Nanogen, Inc. | Dosage immunologique différentiel |
| US6893822B2 (en) | 2001-07-19 | 2005-05-17 | Nanogen Recognomics Gmbh | Enzymatic modification of a nucleic acid-synthetic binding unit conjugate |
| US8012770B2 (en) | 2009-07-31 | 2011-09-06 | Invisible Sentinel, Inc. | Device for detection of antigens and uses thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4320109A (en) * | 1979-06-29 | 1982-03-16 | The University Of Southern California | Immunoradiometric assay employing terminal radionuclide labeling and synthesis of conjugates for such assay |
| JPH081438B2 (ja) * | 1986-05-13 | 1996-01-10 | 三洋化成工業株式会社 | 酵素免疫測定法 |
| US5296347A (en) * | 1991-02-08 | 1994-03-22 | Ciba Corning Diagnostics Corp. | Bridge immunoassay |
| EP0579767B1 (fr) * | 1991-04-11 | 2000-08-23 | Biosite Diagnostics Inc. | Nouveaux conjugues et dosages destines a la detection simultanee de ligands multiples |
| GB9624750D0 (en) * | 1996-11-28 | 1997-01-15 | Univ London | Capture assays |
-
1998
- 1998-12-23 DE DE19859912A patent/DE19859912C2/de not_active Expired - Fee Related
-
1999
- 1999-12-22 EP EP99968373A patent/EP1141725A2/fr not_active Withdrawn
- 1999-12-22 JP JP2000591429A patent/JP2002533724A/ja active Pending
- 1999-12-22 CA CA002353920A patent/CA2353920A1/fr not_active Abandoned
- 1999-12-22 AU AU25390/00A patent/AU773046B2/en not_active Ceased
- 1999-12-22 WO PCT/EP1999/010333 patent/WO2000039581A2/fr not_active Ceased
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10705084B2 (en) | 2009-07-31 | 2020-07-07 | Invisible Sentinel, Inc. | Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof |
| US10495638B2 (en) | 2009-10-09 | 2019-12-03 | Invisible Sentinel, Inc. | Device for detection of analytes and uses thereof |
| US10018626B2 (en) | 2012-03-09 | 2018-07-10 | Invisible Sentinel, Inc. | Methods and compositions for detecting multiple analytes with a single signal |
| AU2013230917B2 (en) * | 2012-03-09 | 2018-11-29 | Invisible Sentinel, Inc. | Methods and compositions for detecting multiple analytes with a single signal |
| EP3578980A1 (fr) * | 2012-03-09 | 2019-12-11 | Invisible Sentinel, Inc. | Procédés et compositions permettant de détecter plusieurs analytes à l'aide d'un seul signal |
| US10732177B2 (en) | 2012-03-09 | 2020-08-04 | Invisible Sentinel, Inc. | Methods and compositions for detecting multiple analytes with a single signal |
| AU2018264112B2 (en) * | 2012-03-09 | 2021-05-27 | Invisible Sentinel, Inc. | Methods and compositions for detecting multiple analytes with a single signal |
| AU2013230917C1 (en) * | 2012-03-09 | 2021-09-16 | Invisible Sentinel, Inc. | Methods and compositions for detecting multiple analytes with a single signal |
| WO2020079638A1 (fr) * | 2018-10-17 | 2020-04-23 | Molecular Devices (Austria) GmbH | Dosages de buvardage de western en temps réel utilisant un transfert d'énergie par résonance de fluorescence (fret) |
| US11604186B2 (en) | 2018-10-17 | 2023-03-14 | Molecular Devices (Austria) GmbH | Real time western blot assays utilizing fluorescence resonance energy transfer (FRET) |
Also Published As
| Publication number | Publication date |
|---|---|
| AU773046B2 (en) | 2004-05-13 |
| WO2000039581A2 (fr) | 2000-07-06 |
| DE19859912A1 (de) | 2000-07-06 |
| WO2000039581A3 (fr) | 2000-11-23 |
| AU2539000A (en) | 2000-07-31 |
| DE19859912C2 (de) | 2001-06-21 |
| EP1141725A2 (fr) | 2001-10-10 |
| JP2002533724A (ja) | 2002-10-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6682648B1 (en) | Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures | |
| EP1255861B1 (fr) | Procedes et trousses de detection de proximite | |
| JP2022522480A (ja) | 免疫測定法で使用するための電気化学発光標識プローブ、当該プローブを使用する方法および該プローブを含むキット | |
| US6265169B1 (en) | Method based on the use of bacteriophages for the detection biological molecules in biological samples | |
| US20110189674A1 (en) | Method for quantifying or detecting dna | |
| JP2016516997A (ja) | 改善されたアッセイ方法 | |
| WO2017181339A1 (fr) | Procédé et kit pour la détection simultanée d'un ligand de protéine et d'un gène | |
| KR20150139582A (ko) | 나노파티클-지원 신호 증폭을 이용한 rna 마이크로칩 검출 | |
| US20050136442A1 (en) | Three dimensional apparatus and method for integrating sample preparation and multiplex assays | |
| CN113195722B (zh) | 与基孔肯雅热病毒e2特异性结合的dna适体及其用途 | |
| AU8903998A (en) | Electrochemical reporter system for detecting analytical immunoassay and mol ecular biology procedures | |
| AU773046B2 (en) | Test system for detecting different markers, and production and use thereof | |
| WO2020014883A1 (fr) | Aptamère d'adn simple brin reconnaissant spécifiquement la tobramycine et application associée | |
| JP2008263963A (ja) | Ｄｎａメチル化測定方法 | |
| US20250354198A1 (en) | Methods for detecting and isolating extracellular vesicles | |
| US20070264652A1 (en) | Method for detection of a sequence | |
| CN100467612C (zh) | 同时检测杂交和免疫反应的方法以及其在诊断方面的应用 | |
| US20050239078A1 (en) | Sequence tag microarray and method for detection of multiple proteins through DNA methods | |
| US20230088664A1 (en) | Method of Detecting Analytes in a Sample | |
| JP4227139B2 (ja) | 生体試料の分析方法、反応液、及び分析装置 | |
| WO2024241162A1 (fr) | Peptide pour conjugaison d'anticorps spécifique à un site et procédé d'utilisation | |
| JPH04286957A (ja) | 遺伝子検出方法 | |
| JP5151709B2 (ja) | Ｄｎａを定量又は検出する方法 | |
| CN116500273A (zh) | 一种基于信号转换与核酸多重等温扩增的b7-h3蛋白检测方法 | |
| JPH06235726A (ja) | 核酸プローブ及び核酸検出方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |