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CA2294627A1 - Procede d'identification et/ou de dosage de substances biologiques, presentes dans un liquide conducteur, dispositif et capteur d'affinite utiles pour la mise en oeuvre de ce procede - Google Patents

Procede d'identification et/ou de dosage de substances biologiques, presentes dans un liquide conducteur, dispositif et capteur d'affinite utiles pour la mise en oeuvre de ce procede Download PDF

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Publication number
CA2294627A1
CA2294627A1 CA002294627A CA2294627A CA2294627A1 CA 2294627 A1 CA2294627 A1 CA 2294627A1 CA 002294627 A CA002294627 A CA 002294627A CA 2294627 A CA2294627 A CA 2294627A CA 2294627 A1 CA2294627 A1 CA 2294627A1
Authority
CA
Canada
Prior art keywords
pnc
identifying
conductive liquid
implementing
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002294627A
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English (en)
Other versions
CA2294627C (fr
Inventor
Jean-Rene Martin
Eliane Souteyrand
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Ecole Centrale de Lyon
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by Individual filed Critical Individual
Publication of CA2294627A1 publication Critical patent/CA2294627A1/fr
Application granted granted Critical
Publication of CA2294627C publication Critical patent/CA2294627C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/305—Electrodes, e.g. test electrodes; Half-cells optically transparent or photoresponsive electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Electrochemistry (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention a pour but de fournir un procédé perfectionné d'identification et/ou de dosage de polynucléotides PN présents dans un milieu liquide conducteur. Ce procédé consiste à faire intervenir un capteur d'affinité comprenant une structure multicouches semi-conducteur/isolant/sondes = polynucléotides PNc complémentaires aux PN du milieu. Selon ce procédé, on sélectionne des sondes PNc non marquées, on polarise le semi-conducteur, on l'éclaire périodiquement, on mesure les variations du potentiel de bande plate induites par un phénomène d'effet de charge directement et essentiellement lié aux appariements spécifiques entre PN et PNc, et enfin on interprète les signaux recueillis en terme d'identification et/ou de dosage des PN. L'invention concerne également le dispositif et le capteur utile pour la mise en oeuvre de ce procédé. Application: analyse en biologie moléculaire, e.g. diagnostic de maladies virales ou génétiques.
CA002294627A 1997-06-11 1998-06-11 Procede d'identification et/ou de dosage de substances biologiques, presentes dans un liquide conducteur, dispositif et capteur d'affinite utiles pour la mise en oeuvre de ce procede Expired - Fee Related CA2294627C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9707530A FR2764702B1 (fr) 1997-06-11 1997-06-11 Procede d'identification et/ou de dosage de substances biologiques, presentes dans un liquide conducteur, dispositif et capteur d'affinite utiles pour la mise en oeuvre de ce procede
FR97/07530 1997-06-11
PCT/FR1998/001213 WO1998057157A1 (fr) 1997-06-11 1998-06-11 Procede d'identification et/ou de dosage de substances biologiques, presentes dans un liquide conducteur, dispositif et capteur d'affinite utiles pour la mise en oeuvre de ce procede

Publications (2)

Publication Number Publication Date
CA2294627A1 true CA2294627A1 (fr) 1998-12-17
CA2294627C CA2294627C (fr) 2009-06-09

Family

ID=9508097

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002294627A Expired - Fee Related CA2294627C (fr) 1997-06-11 1998-06-11 Procede d'identification et/ou de dosage de substances biologiques, presentes dans un liquide conducteur, dispositif et capteur d'affinite utiles pour la mise en oeuvre de ce procede

Country Status (7)

Country Link
US (1) US6475728B1 (fr)
EP (1) EP0988531A1 (fr)
JP (1) JP2002503343A (fr)
AU (1) AU8112898A (fr)
CA (1) CA2294627C (fr)
FR (1) FR2764702B1 (fr)
WO (1) WO1998057157A1 (fr)

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JP2004532382A (ja) * 2000-09-27 2004-10-21 サントル・ナショナル・ドゥ・ラ・ルシェルシュ・シアンティフィク−シーエヌアールエス 複数の異なるポリヌクレオチドおよび／またはペプチド配列を保有する支持体を製造するための方法および装置
US7090757B2 (en) * 2002-02-15 2006-08-15 Ut-Battelle Llc Photoelectrochemical molecular comb
DE10224567B4 (de) * 2002-06-03 2014-10-23 Boehringer Ingelheim Vetmedica Gmbh Sensor-Anordnung und Verfahren zum Betreiben einer Sensor-Anordnung
US8053245B1 (en) 2003-07-29 2011-11-08 Nanotel Biotechnologies, Inc. System and method for detecting biochemicals using hydrated substrates based on liquid crystal polymers
US20050056867A1 (en) * 2003-09-16 2005-03-17 Krzysztof Nauka Surface photovoltage-based sensing of molecules
JP3903183B2 (ja) * 2004-02-03 2007-04-11 独立行政法人物質・材料研究機構 遺伝子検出電界効果デバイスおよびこれを用いた遺伝子多型解析方法
JP2007271266A (ja) * 2004-05-28 2007-10-18 Osaka Univ 光電流による高感度一塩基多型の検出方法
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US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
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AU2007334393A1 (en) 2006-12-14 2008-06-26 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
CN102203282B (zh) 2008-06-25 2014-04-30 生命技术公司 使用大规模fet阵列测量分析物的方法和装置
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
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US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
AU2011226767B1 (en) 2010-06-30 2011-11-10 Life Technologies Corporation Ion-sensing charge-accumulation circuits and methods
CN103080739B (zh) 2010-06-30 2016-12-21 生命科技公司 用于测试isfet阵列的方法和装置
US8487790B2 (en) 2010-06-30 2013-07-16 Life Technologies Corporation Chemical detection circuit including a serializer circuit
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
TWI527245B (zh) 2010-07-03 2016-03-21 生命技術公司 具有微摻雜汲極之化學感測器
EP2617061B1 (fr) 2010-09-15 2021-06-30 Life Technologies Corporation Procédés et appareil de mesure d'analytes
US8685324B2 (en) 2010-09-24 2014-04-01 Life Technologies Corporation Matched pair transistor circuits
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
CN105264366B (zh) 2013-03-15 2019-04-16 生命科技公司 具有一致传感器表面区域的化学传感器
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Also Published As

Publication number Publication date
JP2002503343A (ja) 2002-01-29
US6475728B1 (en) 2002-11-05
FR2764702A1 (fr) 1998-12-18
AU8112898A (en) 1998-12-30
FR2764702B1 (fr) 1999-09-03
WO1998057157A1 (fr) 1998-12-17
CA2294627C (fr) 2009-06-09
EP0988531A1 (fr) 2000-03-29

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Effective date: 20140611