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CA2283251A1 - Device and method for carrying out fluorescence immunoassays - Google Patents

Device and method for carrying out fluorescence immunoassays Download PDF

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Publication number
CA2283251A1
CA2283251A1 CA002283251A CA2283251A CA2283251A1 CA 2283251 A1 CA2283251 A1 CA 2283251A1 CA 002283251 A CA002283251 A CA 002283251A CA 2283251 A CA2283251 A CA 2283251A CA 2283251 A1 CA2283251 A1 CA 2283251A1
Authority
CA
Canada
Prior art keywords
light
base plate
boundary surface
light source
disposed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002283251A
Other languages
French (fr)
Other versions
CA2283251C (en
Inventor
Markus Meusel
Dieter Trau
Andreas Katerkamp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PES GESELLSCHAFT fur MEDIZINISCHE DIAGNOSE-SYSTEME MBH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2283251A1 publication Critical patent/CA2283251A1/en
Application granted granted Critical
Publication of CA2283251C publication Critical patent/CA2283251C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
  • Luminescent Compositions (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The invention relates to a device and a method for carrying out quantitative fluorescence immunoassays by means of evanescent field excitation. Various known biochemical assays of general receptor-ligand systems can be used. For this purpose, light of at least one light source is directed at an angle .alpha. onto the boundary surface of two media which have different refractive indices. A light source is selected which emits substantially monochromatic light with a wavelength which is suitable for exciting a marking substance. The light is directed onto the boundary surface which is disposed between an optically transparent base plate made of a material where the refractive index n1 is greater than the refractive index n2 of the material above the boundary surface, and a cuvette-shaped receiving region for the sample. The receiving region is covered with a covering plate on the side disposed opposite the base plate.
Arranged between base plate and covering plate is at least one functional layer and a detector for detecting the fluorescent light being disposed on the same side of the base plate as the light source.
CA002283251A 1997-03-18 1998-03-11 Device and method for carrying out fluorescence immunoassays Expired - Fee Related CA2283251C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19711281.1 1997-03-18
DE19711281A DE19711281C1 (en) 1997-03-18 1997-03-18 Device for fluorescent specific-binding tests using evanescent field excitation
PCT/DE1998/000729 WO1998041843A2 (en) 1997-03-18 1998-03-11 Device and method for carrying out fluorescence immunotests

Publications (2)

Publication Number Publication Date
CA2283251A1 true CA2283251A1 (en) 1998-09-24
CA2283251C CA2283251C (en) 2007-11-27

Family

ID=7823792

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002283251A Expired - Fee Related CA2283251C (en) 1997-03-18 1998-03-11 Device and method for carrying out fluorescence immunoassays

Country Status (8)

Country Link
EP (1) EP0968412B1 (en)
JP (1) JP2001515592A (en)
AT (1) ATE227425T1 (en)
CA (1) CA2283251C (en)
DE (2) DE19711281C1 (en)
DK (1) DK0968412T3 (en)
RU (1) RU2194972C2 (en)
WO (1) WO1998041843A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11346839B2 (en) 2018-08-03 2022-05-31 Insingulo Ab Method for determining the interaction between a ligand and a receptor

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19933458B4 (en) * 1999-07-15 2015-08-20 Eppendorf Ag Equipment and systems for handling liquid samples
DE10001116C2 (en) * 2000-01-13 2002-11-28 Meinhard Knoll Device and method for the optical or electrochemical quantitative determination of chemical or biochemical substances in liquid samples
DE10058095C2 (en) * 2000-11-03 2003-12-18 Fraunhofer Ges Forschung Device for the determination of analytes by chemiluminescence
DE10105711B4 (en) * 2001-02-08 2005-03-10 Ibidi Gmbh Sample carriers for chemical and biological samples
DE10307802A1 (en) * 2003-02-24 2004-09-09 Advalytix Ag Apparatus for the analysis of specific binding reactions, between protein or DNA probe macro molecules and a fluid containing sample macro molecules, has a spot carrier which is transparent and non-luminescent
DE102004022263A1 (en) * 2004-05-06 2005-12-15 Clondiag Chip Technologies Gmbh Apparatus and method for detecting molecular interactions
RU2280247C1 (en) * 2005-06-30 2006-07-20 Общество с ограниченной ответственностью "Биочип-Аналитика" (ООО "Биочип-Аналитика") Bio-chip for fluorescent and luminescent analysis
JP2008032420A (en) * 2006-07-26 2008-02-14 National Institute Of Advanced Industrial & Technology Method and member for reducing background fluorescence in evanescent excitation fluorescence observation
DE102007019695B4 (en) * 2007-04-24 2009-08-13 Analytik Jena Ag Cuvette for the optical analysis of small volumes
DE102010038431A1 (en) 2010-07-26 2012-01-26 Diasys Diagnostic Systems Gmbh Measuring cassette and measuring device for the detection of target molecules in a liquid sample by measuring fluorescence emission after excitation in the evanescent field
DE102022207704A1 (en) 2022-07-27 2024-02-01 Robert Bosch Gesellschaft mit beschränkter Haftung Distributor unit for an analysis device for analyzing a sample contained in a microfluidic cartridge, analysis device and method for producing a body unit for a distribution unit

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3814370A1 (en) * 1988-04-28 1989-11-09 Boehringer Mannheim Gmbh TEST PROVIDER FOR THE ANALYSIS OF A SAMPLING FLUID, METHOD FOR CARRYING OUT SUCH ANALYSIS AND METHOD OF PRODUCTION
SE462408B (en) * 1988-11-10 1990-06-18 Pharmacia Ab OPTICAL BIOSENSOR SYSTEM USING SURFACE MONITORING RESONSE FOR THE DETECTION OF A SPECIFIC BIOMOLIC CYCLE, TO CALIBRATE THE SENSOR DEVICE AND TO CORRECT FOUND BASELINE OPERATION IN THE SYSTEM
GB8827853D0 (en) * 1988-11-29 1988-12-29 Ares Serono Res & Dev Ltd Sensor for optical assay
DE4121493A1 (en) * 1991-06-28 1993-01-07 Draegerwerk Ag ANALYSIS DEVICE FOR THE QUANTITATIVE, IMMUNOLOGICAL DETERMINATION OF POLLUTANTS
RU2039986C1 (en) * 1992-03-23 1995-07-20 Институт иммунобиотехнологии Immunofluorescent method for concurrently determining antigens
EP0700514B1 (en) * 1993-05-18 2001-11-28 University of Utah Research Foundation Apparatus and methods for multianalyte homogeneous fluoroimmunoassays
DE19611025A1 (en) * 1996-03-20 1997-09-25 Inst Chemo Biosensorik Optical fiber optic sensor based on the resonant optical excitation of surface plasma waves
DE19628002C1 (en) * 1996-07-11 1997-12-18 Inst Chemo Biosensorik Device and method for carrying out fluorescence immunoassays

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11346839B2 (en) 2018-08-03 2022-05-31 Insingulo Ab Method for determining the interaction between a ligand and a receptor

Also Published As

Publication number Publication date
CA2283251C (en) 2007-11-27
EP0968412A2 (en) 2000-01-05
WO1998041843A3 (en) 1998-11-19
ATE227425T1 (en) 2002-11-15
DK0968412T3 (en) 2003-03-10
WO1998041843A2 (en) 1998-09-24
RU2194972C2 (en) 2002-12-20
EP0968412B1 (en) 2002-11-06
DE59806188D1 (en) 2002-12-12
DE19711281C1 (en) 1998-04-16
JP2001515592A (en) 2001-09-18

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MKLA Lapsed

Effective date: 20160311

MKLA Lapsed

Effective date: 20160311