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CA2268004A1 - Systeme de transformation dans candida utilis - Google Patents

Systeme de transformation dans candida utilis Download PDF

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Publication number
CA2268004A1
CA2268004A1 CA002268004A CA2268004A CA2268004A1 CA 2268004 A1 CA2268004 A1 CA 2268004A1 CA 002268004 A CA002268004 A CA 002268004A CA 2268004 A CA2268004 A CA 2268004A CA 2268004 A1 CA2268004 A1 CA 2268004A1
Authority
CA
Canada
Prior art keywords
candida utilis
gene
yeast
defective
host
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002268004A
Other languages
English (en)
Inventor
Luis Rodriguez Menocal
Francisco Pablo Chavez Espinoza
Maria Elena Gonzalez Martinez
Tanilo Rivero Baeza
Liliana Besabe Tuero
Edenia Paifer Reyes
Julio Marcos Delgado Boada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centro de Ingenieria Genetica y Biotecnologia CIGB
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2268004A1 publication Critical patent/CA2268004A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un système de transformation utile pour exprimer des protéines hétérologues dans la levure Candida utilis, ce système de transformation se fondant sur l'obtention de mutants auxotrophiques de cette espèce ainsi que sur l'isolement de différents gènes, à partir d'une bibliothèque génomique, qui complètent lesdites auxotrophies. Le système de transformation consiste à utiliser en tant que hôtes de nouveaux mutants auxotrophiques obtenus à partir de la levure NRRL Y-1084 de Candida utilis, lesquels sont déficients essentiellement dans les voies biosynthétiques de uracyl et histidine, lesquels sont transformés avec des plasmides qui contiennent en tant que marqueurs de sélection les gènes $(URA3) et HIS3 de $(Candida utilis). Un autre aspect de l'invention concerne l'isolement du gène codant pour l'enzyme sucrose invertase ou .beta.-fructofuranosidase de Candida utilis, ainsi que l'identification de séquences de promotion, de signalisation de sécrétion et de terminaison dudit gène INV1. Ces séquences peuvent être utilisées de manière avantageuse pour effectuer l'expression de protéines hétérologues dans cette levure.
CA002268004A 1996-10-03 1997-10-03 Systeme de transformation dans candida utilis Abandoned CA2268004A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CU1996082A CU22722A1 (es) 1996-10-03 1996-10-03 Sistema de transformación para la expresión de genes heterólogos en la levadura candida utilis
CU82/96 1996-10-03
PCT/CU1997/000005 WO1998014600A1 (fr) 1996-10-03 1997-10-03 SYSTEME DE TRANSFORMATION DANS $i(CANDIDA UTILIS)

Publications (1)

Publication Number Publication Date
CA2268004A1 true CA2268004A1 (fr) 1998-04-09

Family

ID=5459318

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002268004A Abandoned CA2268004A1 (fr) 1996-10-03 1997-10-03 Systeme de transformation dans candida utilis

Country Status (9)

Country Link
EP (1) EP0956356A1 (fr)
JP (1) JP2001501475A (fr)
CN (1) CN1237208A (fr)
AU (1) AU744698B2 (fr)
BR (1) BR9713313A (fr)
CA (1) CA2268004A1 (fr)
CU (1) CU22722A1 (fr)
RU (1) RU2235127C2 (fr)
WO (1) WO1998014600A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003047471A (ja) * 2001-08-01 2003-02-18 Kirin Brewery Co Ltd 異種タンパク質の分泌生産に優れた改変酵母およびその酵母を用いた異種タンパク質の製造法
PE20031013A1 (es) 2002-03-26 2004-02-05 Ajinomoto Kk CANDIDA UTILIS QUE PRODUCE y-GLUTAMILCISTEINA
EP1505149B1 (fr) * 2002-04-26 2009-01-07 Kirin Pharma Kabushiki Kaisha Levure methylotrophe produisant une chaine de sucre de type mammifere
JP2005073638A (ja) 2003-09-02 2005-03-24 Ajinomoto Co Inc キャンディダ・ユティリスのグルタチオン合成酵素をコードする遺伝子
CN100347287C (zh) * 2004-09-30 2007-11-07 汪和睦 一种重组多形汉逊酵母菌及其构建方法与应用
JP5954178B2 (ja) 2010-10-05 2016-07-20 味の素株式会社 γ−Ｇｌｕ−Ａｂｕを含有する酵母及び酵母エキス、並びにそれらの製造方法
WO2015005378A1 (fr) 2013-07-12 2015-01-15 味の素株式会社 LEVURE À FORTE TENEUR EN Abu, γ-Glu-Abu, ET/OU γ-Glu-Abu-Gly
CN103468594B (zh) * 2013-07-31 2015-06-17 浙江科峰生物技术有限公司 一种产朊假丝酵母菌株及其应用
EP4317169A4 (fr) 2021-03-22 2025-12-17 Kaneka Corp Procédé de production d'une protéine étrangère à l'aide d'escherichia coli
JPWO2022210308A1 (fr) 2021-03-29 2022-10-06
KR102556901B1 (ko) * 2021-05-25 2023-07-17 국립낙동강생물자원관 담수에서 분리한 항균력 및 식물생장촉진능을 가지는 에드니아 속 nnibrfg15114 균주 및 이의 용도
CN117651776A (zh) 2021-07-16 2024-03-05 株式会社钟化 使用了大肠杆菌的质粒dna的制造方法
CN115838645B (zh) * 2022-09-15 2024-03-08 天津大学 一种高产乳清酸的酵母菌株及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59162884A (ja) * 1983-03-09 1984-09-13 Kohjin Co Ltd 新規な雑種プラスミドおよびそれを含有する微生物
NZ207925A (en) * 1983-04-25 1988-05-30 Genentech Inc Yeast expression vehicle consisting of a yeast promoter and signal peptide encoding region linked to a heterologus peptide coding region; expression and culture
US5204252A (en) * 1989-02-08 1993-04-20 Henkel Research Corporation Candida tropicalis transformation system
JPH08173170A (ja) * 1994-05-25 1996-07-09 Kirin Brewery Co Ltd キャンディダ・ユティリス酵母の形質転換系、およびそれによる異種遺伝子の発現

Also Published As

Publication number Publication date
RU2235127C2 (ru) 2004-08-27
BR9713313A (pt) 2000-10-24
CU22722A1 (es) 2002-02-28
EP0956356A1 (fr) 1999-11-17
AU4548597A (en) 1998-04-24
WO1998014600A1 (fr) 1998-04-09
CN1237208A (zh) 1999-12-01
AU744698B2 (en) 2002-02-28
JP2001501475A (ja) 2001-02-06

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued