CA2253872A1 - Procede et dispositif permettant de modifier la pression osmotique des leucocytes souches conserves par cryogenie - Google Patents
Procede et dispositif permettant de modifier la pression osmotique des leucocytes souches conserves par cryogenie Download PDFInfo
- Publication number
- CA2253872A1 CA2253872A1 CA002253872A CA2253872A CA2253872A1 CA 2253872 A1 CA2253872 A1 CA 2253872A1 CA 002253872 A CA002253872 A CA 002253872A CA 2253872 A CA2253872 A CA 2253872A CA 2253872 A1 CA2253872 A1 CA 2253872A1
- Authority
- CA
- Canada
- Prior art keywords
- aseptic
- bag
- stem cells
- white
- white stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 78
- 230000003204 osmotic effect Effects 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 8
- 230000002338 cryopreservative effect Effects 0.000 claims abstract description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 36
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 22
- 239000011780 sodium chloride Substances 0.000 claims description 18
- 210000004027 cell Anatomy 0.000 claims description 14
- 239000003638 chemical reducing agent Substances 0.000 claims description 10
- 239000002577 cryoprotective agent Substances 0.000 claims description 10
- 230000003750 conditioning effect Effects 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 4
- 238000010606 normalization Methods 0.000 claims description 4
- 229920002307 Dextran Polymers 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims description 3
- 230000001225 therapeutic effect Effects 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 2
- 230000009467 reduction Effects 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 238000010257 thawing Methods 0.000 claims description 2
- 238000005204 segregation Methods 0.000 claims 6
- 238000011010 flushing procedure Methods 0.000 claims 2
- 238000000926 separation method Methods 0.000 claims 1
- 239000012895 dilution Substances 0.000 abstract description 5
- 238000010790 dilution Methods 0.000 abstract description 5
- 239000003085 diluting agent Substances 0.000 description 18
- 239000000243 solution Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 210000001185 bone marrow Anatomy 0.000 description 2
- 238000005138 cryopreservation Methods 0.000 description 2
- 230000003169 placental effect Effects 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002433 mononuclear leukocyte Anatomy 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009919 sequestration Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La présente invention concerne un procédé, un appareil et un produit obtenu à partir de leucocytes souches conservés par cryogénie et convenant à la transfusion. On a réduit la pression osmotique de ces leucocytes souches en réalisant une dilution contrôlée d'un conservateur cryogénique provenant des leucocytes souches.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US81336497A | 1997-03-07 | 1997-03-07 | |
| US08/813,364 | 1997-03-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2253872A1 true CA2253872A1 (fr) | 1998-09-11 |
Family
ID=25212173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002253872A Abandoned CA2253872A1 (fr) | 1997-03-07 | 1998-03-06 | Procede et dispositif permettant de modifier la pression osmotique des leucocytes souches conserves par cryogenie |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0967928A4 (fr) |
| JP (1) | JP2001518079A (fr) |
| AU (1) | AU6538898A (fr) |
| CA (1) | CA2253872A1 (fr) |
| WO (1) | WO1998038940A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10117205A1 (de) * | 2001-04-06 | 2002-10-17 | Martin Winkemann | Vorrichtung zur Vorbereitung von Zellen für die Kryokonservierung |
| JP5802298B2 (ja) | 2014-03-25 | 2015-10-28 | テルモ株式会社 | 凍結保存細胞からの生細胞の回収方法およびシステム |
| US9587215B2 (en) * | 2014-08-07 | 2017-03-07 | General Electric Company | Devices, systems and methods for automated transfer of a sample |
| JP6486246B2 (ja) * | 2015-08-28 | 2019-03-20 | テルモ株式会社 | 凍結保存細胞からの生細胞の回収方法およびシステム |
| WO2021201029A1 (fr) * | 2020-03-31 | 2021-10-07 | Ｃｅｌｌ Ｅｘｏｓｏｍｅ Ｔｈｅｒａｐｅｕｔｉｃｓ株式会社 | Procédé de conservation de cellules |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4306556A (en) * | 1980-02-07 | 1981-12-22 | Rensselaer Polytechnic Institute | Method and apparatus for storing and preparing cryopreserved blood |
| US4900322A (en) * | 1986-09-22 | 1990-02-13 | Adams James D | Blood component pooling valve and kit |
| US5595866A (en) * | 1994-05-27 | 1997-01-21 | Methodist Hospital Of Indiana, Inc. | Step-wise method to remove cryoprotectant from sperm |
-
1998
- 1998-03-06 AU AU65388/98A patent/AU6538898A/en not_active Abandoned
- 1998-03-06 WO PCT/US1998/003936 patent/WO1998038940A1/fr not_active Ceased
- 1998-03-06 JP JP53862098A patent/JP2001518079A/ja active Pending
- 1998-03-06 EP EP98911437A patent/EP0967928A4/fr not_active Withdrawn
- 1998-03-06 CA CA002253872A patent/CA2253872A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998038940A1 (fr) | 1998-09-11 |
| AU6538898A (en) | 1998-09-22 |
| EP0967928A4 (fr) | 2003-05-21 |
| EP0967928A1 (fr) | 2000-01-05 |
| JP2001518079A (ja) | 2001-10-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |