CA2197493A1 - Methode de sequencage des proteines par classement des epitopes et cartographie de restriction des proteines - Google Patents
Methode de sequencage des proteines par classement des epitopes et cartographie de restriction des proteinesInfo
- Publication number
- CA2197493A1 CA2197493A1 CA 2197493 CA2197493A CA2197493A1 CA 2197493 A1 CA2197493 A1 CA 2197493A1 CA 2197493 CA2197493 CA 2197493 CA 2197493 A CA2197493 A CA 2197493A CA 2197493 A1 CA2197493 A1 CA 2197493A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleotide sequence
- sequence
- nucleotide
- modified
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 230000009145 protein modification Effects 0.000 description 1
- 238000000734 protein sequencing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 1
- 235000016491 selenocysteine Nutrition 0.000 description 1
- 229940055619 selenocysteine Drugs 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- TYFQFVWCELRYAO-UHFFFAOYSA-L suberate(2-) Chemical compound [O-]C(=O)CCCCCCC([O-])=O TYFQFVWCELRYAO-UHFFFAOYSA-L 0.000 description 1
- 230000019635 sulfation Effects 0.000 description 1
- 238000005670 sulfation reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 230000034512 ubiquitination Effects 0.000 description 1
- 238000010798 ubiquitination Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Peptides Or Proteins (AREA)
Abstract
La présente invention concerne des procédés d'identification d'une séquence d'acides aminés d'une protéine et de caractérisation des événements post-traduction survenant sur la protéine au cours du classement d'épitopes suivant une cartographie des sites de restriction. L'invention concerne également des anticorps modifiés contenant des sites d'enzymes de restriction, et qui sont capables de reconnaître des acides aminés et de se lier à eux en tant qu'épitope ou en tant qu'épitopes modifiés après traduction.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29413394A | 1994-08-22 | 1994-08-22 | |
| US08/294,133 | 1994-08-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2197493A1 true CA2197493A1 (fr) | 1996-02-29 |
Family
ID=23132029
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2197493 Abandoned CA2197493A1 (fr) | 1994-08-22 | 1995-08-22 | Methode de sequencage des proteines par classement des epitopes et cartographie de restriction des proteines |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0778777A4 (fr) |
| JP (1) | JPH10507344A (fr) |
| CA (1) | CA2197493A1 (fr) |
| WO (1) | WO1996005847A1 (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6821738B2 (en) | 1999-01-20 | 2004-11-23 | The Board Of Regents For Oklahoma State University | Broad spectrum bio-detection of nerve agents, organophosphates, and other chemical warfare agents |
| GB9904395D0 (en) * | 1999-02-25 | 1999-04-21 | Fluorescience Ltd | Protein assay |
| US6972198B2 (en) * | 1999-02-26 | 2005-12-06 | Cyclacel, Ltd. | Methods and compositions using protein binding partners |
| DE10054055A1 (de) * | 2000-10-31 | 2002-05-23 | Nmi Univ Tuebingen | Verfahren zur Analyse von Proteinen |
| US7618788B2 (en) | 2002-05-10 | 2009-11-17 | Millipore Corporation | Proteome epitope tags and methods of use thereof in protein modification analysis |
| US7460960B2 (en) | 2002-05-10 | 2008-12-02 | Epitome Biosystems, Inc. | Proteome epitope tags and methods of use thereof in protein modification analysis |
| US7855057B2 (en) | 2006-03-23 | 2010-12-21 | Millipore Corporation | Protein splice variant/isoform discrimination and quantitative measurements thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2107730C1 (ru) * | 1990-05-04 | 1998-03-27 | Чирон Корпорейшн | Молекулярный зонд |
| US5348633A (en) * | 1993-01-22 | 1994-09-20 | Northeastern University | Method for quantitating trace amounts of an analyte in a sample by affinity capillary electrophoresis |
-
1995
- 1995-08-22 JP JP8508272A patent/JPH10507344A/ja active Pending
- 1995-08-22 CA CA 2197493 patent/CA2197493A1/fr not_active Abandoned
- 1995-08-22 WO PCT/US1995/010668 patent/WO1996005847A1/fr not_active Ceased
- 1995-08-22 EP EP95930901A patent/EP0778777A4/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP0778777A4 (fr) | 1999-01-27 |
| EP0778777A1 (fr) | 1997-06-18 |
| WO1996005847A1 (fr) | 1996-02-29 |
| JPH10507344A (ja) | 1998-07-21 |
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