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CA2194177A1 - Procede de repliement de proteines telles que l'hirudine ou le facteur de croissance epidermique de recombinaison - Google Patents

Procede de repliement de proteines telles que l'hirudine ou le facteur de croissance epidermique de recombinaison

Info

Publication number
CA2194177A1
CA2194177A1 CA002194177A CA2194177A CA2194177A1 CA 2194177 A1 CA2194177 A1 CA 2194177A1 CA 002194177 A CA002194177 A CA 002194177A CA 2194177 A CA2194177 A CA 2194177A CA 2194177 A1 CA2194177 A1 CA 2194177A1
Authority
CA
Canada
Prior art keywords
protein
process according
proteins
correct
folded protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002194177A
Other languages
English (en)
Inventor
Jui Yoa Chang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2194177A1 publication Critical patent/CA2194177A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne un procédé permettant de préparer une protéine repliée correctement, biologiquement active, en présence d'un agent dénaturant tel que l'urée ou l'hydrochlorure de guanidine, et de séparer la protéine correctement repliée directement de celui-ci. Ce procédé peut être mis en oeuvre, par exemple, lors de la renaturation de protéines de recombinaison telles que l'hirudine ou le facteur de croissance épidermique.
CA002194177A 1994-07-25 1995-07-12 Procede de repliement de proteines telles que l'hirudine ou le facteur de croissance epidermique de recombinaison Abandoned CA2194177A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP94810437 1994-07-25
EP94810437.7 1994-07-25

Publications (1)

Publication Number Publication Date
CA2194177A1 true CA2194177A1 (fr) 1996-02-08

Family

ID=8218291

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002194177A Abandoned CA2194177A1 (fr) 1994-07-25 1995-07-12 Procede de repliement de proteines telles que l'hirudine ou le facteur de croissance epidermique de recombinaison

Country Status (9)

Country Link
EP (1) EP0804461A1 (fr)
JP (1) JPH11505507A (fr)
AU (1) AU3109795A (fr)
CA (1) CA2194177A1 (fr)
FI (1) FI970230A0 (fr)
IL (1) IL114699A0 (fr)
NO (1) NO970081D0 (fr)
WO (1) WO1996003425A1 (fr)
ZA (1) ZA956137B (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3734634B2 (ja) * 1999-03-03 2006-01-11 ヒゲタ醤油株式会社 タンパク質の活性化方法及び該装置
US7521479B2 (en) 2001-04-16 2009-04-21 Panacea Pharmaceuticals, Inc. Methods of treating prion disease in mammals
AU2003223936A1 (en) * 2002-05-17 2003-12-02 Kobenhavns Universitet Method for purifying denatured proteins having a desired disulfide bond configuration

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR79124B (fr) * 1982-12-22 1984-10-02 Genentech Inc
ES8800957A1 (es) * 1985-02-22 1987-12-01 Monsanto Co Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
WO1992014832A1 (fr) * 1991-02-26 1992-09-03 Ajinomoto Co., Inc. Procedes pour la purification de fdlb humains
US5290920A (en) * 1992-04-16 1994-03-01 Allelix Biopharmaceuticals Inc. Method of purifying human epidermal growth factor

Also Published As

Publication number Publication date
FI970230A7 (fi) 1997-01-20
JPH11505507A (ja) 1999-05-21
AU3109795A (en) 1996-02-22
FI970230L (fi) 1997-01-20
EP0804461A1 (fr) 1997-11-05
WO1996003425A1 (fr) 1996-02-08
FI970230A0 (fi) 1997-01-20
NO970081L (no) 1997-01-09
NO970081D0 (no) 1997-01-09
ZA956137B (en) 1996-03-07
IL114699A0 (en) 1995-11-27

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Legal Events

Date Code Title Description
FZDE Discontinued