[go: up one dir, main page]

CA2192324A1 - Recombinant virus and related method of quantitating same - Google Patents

Recombinant virus and related method of quantitating same

Info

Publication number
CA2192324A1
CA2192324A1 CA002192324A CA2192324A CA2192324A1 CA 2192324 A1 CA2192324 A1 CA 2192324A1 CA 002192324 A CA002192324 A CA 002192324A CA 2192324 A CA2192324 A CA 2192324A CA 2192324 A1 CA2192324 A1 CA 2192324A1
Authority
CA
Canada
Prior art keywords
quantitating
nucleic acid
tagged
same
recombinant virus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002192324A
Other languages
French (fr)
Other versions
CA2192324C (en
Inventor
Venkatachala Natarajan
Norman P. Salzman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Georgetown University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2192324A1 publication Critical patent/CA2192324A1/en
Application granted granted Critical
Publication of CA2192324C publication Critical patent/CA2192324C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • AIDS & HIV (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates generally to the quantitation of virus and viral nucleic acid. The invention relates to methods for quantitating an amount of virus present in a sample, comprising introducing into the sample a composition comprising a genetically tagged viral nucleic acid, isolating said wild type and said tagged nucleic acid, and quantitating said wild type and said tagged nucleic acid. The invention also relates to genetically tagged retroviral nucleic acid comprising a tag sequence, including insertions and deletions.
CA002192324A 1994-06-10 1994-06-10 Recombinant virus and related method of quantitating same Expired - Fee Related CA2192324C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1994/006631 WO1995034684A1 (en) 1994-06-10 1994-06-10 Recombinant virus and related method of quantitating same

Publications (2)

Publication Number Publication Date
CA2192324A1 true CA2192324A1 (en) 1995-12-21
CA2192324C CA2192324C (en) 2006-10-10

Family

ID=22242665

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002192324A Expired - Fee Related CA2192324C (en) 1994-06-10 1994-06-10 Recombinant virus and related method of quantitating same

Country Status (4)

Country Link
JP (1) JPH10505224A (en)
AU (1) AU692792B2 (en)
CA (1) CA2192324C (en)
WO (1) WO1995034684A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5939262A (en) * 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US6429007B1 (en) 1997-05-02 2002-08-06 BIOMéRIEUX, INC. Nucleic acid amplification reaction station for disposable test devices
US5989499A (en) * 1997-05-02 1999-11-23 Biomerieux, Inc. Dual chamber disposable reaction vessel for amplification reactions
US6410275B1 (en) 1997-05-02 2002-06-25 Biomerieux, Inc. Disposable test devices for performing nucleic acid amplification reactions
DE19737442C2 (en) * 1997-08-22 1999-06-24 Olfert Landt Tib Molbiol Synth Protein-coated polyribonucleic acids, a process for their preparation and their use
EP1012344A4 (en) * 1997-09-12 2004-08-11 Public Health Res Instituteof NON-COMPETITIVE CO-AMPLIFICATION PROCESSES
US8012719B2 (en) 1998-11-17 2011-09-06 Paolo Lusso Method for the quantitative detection of nucleic acids
IT1303767B1 (en) 1998-11-17 2001-02-23 San Raffaele Centro Fond METHOD OF QUANTIFICATION OF NUCLEIC ACIDS.
EP1194588A2 (en) * 1999-04-20 2002-04-10 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Diagnostic standards for virus detection and quantification
BRPI0600715B8 (en) * 2006-03-16 2021-07-27 Fundacao Oswaldo Cruz artificial calibrator virus (vca), method to ensure detection and/or quantification of viral load, viral detection kit and process for obtaining artificial calibrator virus
JPWO2009123347A1 (en) 2008-03-31 2011-07-28 日本たばこ産業株式会社 Virus quantification method
GB201002434D0 (en) 2010-02-12 2010-03-31 Plant Bioscience Ltd Non-infectious RNA source

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5256545A (en) * 1987-03-30 1993-10-26 Board Of Regents, The University Of Texas System Sterol Regulatory Elements
US5278056A (en) * 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
US5180666A (en) * 1991-06-27 1993-01-19 Wayne State University Method and cell line for testing mutagenicity of a chemical
US5262311A (en) * 1992-03-11 1993-11-16 Dana-Farber Cancer Institute, Inc. Methods to clone polyA mRNA

Also Published As

Publication number Publication date
AU7512394A (en) 1996-01-05
WO1995034684A1 (en) 1995-12-21
JPH10505224A (en) 1998-05-26
CA2192324C (en) 2006-10-10
AU692792B2 (en) 1998-06-18

Similar Documents

Publication Publication Date Title
CA2192324A1 (en) Recombinant virus and related method of quantitating same
WO1996022308A3 (en) Appetite suppression factor and related methods
AU4200797A (en) Cellulase variants
AU2929495A (en) Nucleic acid sequence amplification method
EP0745128B8 (en) Hepatitis b virus mutants, reagents and methods for detection
EP0756010A3 (en) Method for detecting, identifying, and quantitating organisms and viruses
NZ320276A (en) Method and preparations for stabilizing biological materials by drying methods without freezing
EP0656954A4 (en) Method for producing tagged genes, transcripts, and proteins.
CA2094144A1 (en) Methods and applications for efficient genetic suppressor elements
DE69939945D1 (en) METHOD OF ISOLATING STEM CELLS
AU4978496A (en) Encapsidated recombinant poliovirus nucleic acid
CA2166291A1 (en) Htlv-iinra compositions and assays for detecting htlv infection
EP1826271A3 (en) Thermophilic fungal expression system
EP1304383A3 (en) Sugarcane bacilliform virus promoter
AU1686395A (en) (deltex) proteins, nucleic acids, and antibodies, and related methods and compositions
CA2091181A1 (en) Cloning of chicken anaemia dna
CA2263713A1 (en) Lyophilization of cultured human cells to preserve rna and dna
WO1987001374A3 (en) Method and means for sorting and identifying biological information
WO1995015391A3 (en) Aspergillus expression system
AU6913394A (en) Nucleic acid tagged immunoassay
EP0357350A3 (en) Novel xanthene dyes
CA2220226A1 (en) Improved methods for transfecting t cells
AU2667995A (en) Chimerical peptide-nucleic acid fragment, process for producing the same and its use for appropriately introducing nucleic acids into cell organelles and cells
CA2199835A1 (en) Method for preparing nucleic acids for analysis and kits useful therefor
CA2231738A1 (en) Stress resistant plant in which cell death suppressing gene is introduced and method for producing the same

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20140610