CA2182164A1 - Method of preserving tissues and organs - Google Patents
Method of preserving tissues and organsInfo
- Publication number
- CA2182164A1 CA2182164A1 CA002182164A CA2182164A CA2182164A1 CA 2182164 A1 CA2182164 A1 CA 2182164A1 CA 002182164 A CA002182164 A CA 002182164A CA 2182164 A CA2182164 A CA 2182164A CA 2182164 A1 CA2182164 A1 CA 2182164A1
- Authority
- CA
- Canada
- Prior art keywords
- pressure
- space
- oxygen
- closed
- accordance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 31
- 210000000056 organ Anatomy 0.000 title claims abstract description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 57
- 239000001301 oxygen Substances 0.000 claims abstract description 57
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 57
- 239000012298 atmosphere Substances 0.000 claims abstract description 20
- 238000012432 intermediate storage Methods 0.000 claims abstract description 10
- 238000002054 transplantation Methods 0.000 claims abstract description 6
- 230000007613 environmental effect Effects 0.000 claims description 4
- 239000007789 gas Substances 0.000 claims description 2
- 230000009469 supplementation Effects 0.000 claims description 2
- 238000007664 blowing Methods 0.000 claims 1
- 229960003903 oxygen Drugs 0.000 claims 1
- 230000000694 effects Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 108010083687 Ion Pumps Proteins 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
- A01N1/165—Pressure processes, e.g. following predefined pressure changes over time
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A method is described for preserving tissues and organs, in particular of tissues and organs for transplantation, in which the tissue or organ to be treated is temporarily stored in a well-cooled state, in particular at a temperature in the range from -3°C to 3°C, for a predeterminable time in an oxygen atmosphere, in a space which can be closed off in air tight manner, wherein the oxygen atmosphere is built up in the space which can be sealed off in air tight manner with a pressure which lies above the atmospheric pressure, and wherein the pressure in the closed space is kept at a pressure lying above the atmospheric pressure for the whole of the further period of intermediate storage.
Description
2~2~ ~4 cell structure, even several days after ~emoval fro~ a body, and are thus still suitable for a transplantation.
The build-up of the oxygen atmosphere can take place in the method of the invention after the ga~ corresponding to the environmental or ambient atmosphe~e has been removed ~rom the closeable space in a preceding step. It is, however, also possible to blow oxygen directly with excess pressure into the space and to thereby displace the ga~ corresponding to the environmental atmosphere out of the space. It is important that an oxygen atmosphere is provided in some way or another in the closa~le space with a pressure which is elevated relative to the environment.
In this respect the oxygen atmosphere provided in the space should advantageously have a degree of purity of at least 50 ~, in particular of more than 80 % and prefera~ly of at least 93 %, whereby a situation is achieved in which an extre~ely pronounced preser~ation effect results for the treated tissue.
The method of the inven~ion can be carried out particularly effectively when the evacuation process is carrled out over a time interval of one hour, with the pressure within the closed space sinking to a depression of 50 to loO mbar, and if the build-up o~ the oxygen atmosphere within the closed space then takes place to a pre~sure o~ 6 to 11 bar.
During the period of intermediate storage a continuous or di~continuous supplementation of the oxygen atmosphere in the closed space can be effected, ;~ith oxygen preferably being continuously introduced into the closed space at a pressure which is higher than a predeterminable discharge pressure so that a continuous through-flow sets in as a result of the pressure di~ference. Through this preferably continuous supplementatlon of the oxygen in the closed-off ?
2~82164 The invention relates to a method of preserving tissues and organs, in particular of tissues and organ~ for transplan-tation.
With tissues and organs for transplantation the problem always exists that the transplantation must take place within a very short time, generally within a few hours after the removal of the tissue or organ from a body. An attempt is admittedly made to extend the time interval in which the tissue or organs can be transplanted into a new body by main-taining specific temperatures. However, this is only possible to an inadequate degree.
It is the object of the present invention to set ~orth a method by which tiSSIle and organs, in particular tissues and organs for transplantatlon, can ~e preserved over a long period of time without their cell structure changing.
This o~ject i~ satisfied in accordance with the invention essentially by a method in which the organs or tissues are te~porarily stored in a well-cooled state, in particular at a temperature in the range from -~C to 3~C, for a predeter-minable time in an oxygeli atmosphere, in a space which can be closed of~ in air tight ~anner, wherein the oxygen atmo-sphere is built up in the ~pace which can be sealed o~f in air tight manner with a pressure which lies above the atmo-spheric pressure, and wherein the pressure in the closed space is kept at a pressure lying a~ove the atmospheric pressure for the whole of the ~urther period of intermediate storage.
It has surprisingly been ~ound that tissues and organs which are treated by this method do not have any changes in their ; ' ~ , space the original oxygen content in the space can be maintained during the entire period of intermediate storage so that a particularly good preservation effect ~ets in.
Further advantageous embodiments of the invention are evident from the subordinate claims.
The in~ention will be explained in more detail by way o~
example in the followlng with reference to the drawinas in which are shown.
Figure 1 a schematic side-view o~ an apparatus ~or carrying - out the method o~ the invention, and Figure 2 a schematic illustration to ~a~e clear the ~anner of operation of the apparatus for carryinq the method o~ the invention.
~igure 1 shows an apparatus in accordance with the inven-tion, ~ith a housing of closed design consisting of a welded construction, having an opening 3 in order to place tissue or organs to be treated into the housing 1 in a manner such that oxygen has access to them from substantially all sides.
The opening 3 can be sealingly closed o~f by means of the housing door 4.
An oxygen generator 5, an evacuation pump 6 and also an oxyqen tank 7 and an f.~lectronic control unit 8 are mounted on the housing l The housing 1 furthermore has, as shown schematically in Figure 2, an evacuation opening 9 and an oxygen inlet opening 10 at the roof side and also an oxygen extraction opening 11 at the floor side.
.
.. .
2~ 8~1 64 The evacua~ion pump 6 is connected to the evacuation opening 9 via a pipe conduit or hose 14 closable by means of a solenoid valve 13.
A pipe conduit or a hose 15, which can likewise be closed by means of a solenoid valve 16, branc~es off fro~ the pipe conduit 14 between the solenoid valve 13 and the evacuation opening 9 and opens lnto the environmental atmosphPre.
The oxygen generator 5 is con~ected via a pipe conduit or a hose 17 to the inle~ opening 10, with the suction end of the pipe conduit 14 being closable by means of a solenoid valve 18.
A heat exchanger 19 in the form of a Peltier ele~ent is furthermore provided between the oxygen generator 5 and the inlet opening lO. Here the temperature of the oxygen ls measured and the oxygen is cooled down to ca. 0C. The cooling of the oxygen contribu~es significantly to the preservation of the organs and tissues.
A solenoid valve 20 is further~ore provided ~etween the oxygen generator 5 or the ~eat exc~anger 19 and the inlet opening lO.
A further pipe conduit or a hose 21, which can be closed by a solenoid valve 22 an~ leads to ~he oxygen tank 7, branches off from the pipe conduit 17 between the solenoid valve Z~
and the heat exchanger 19. The pipe ccnduit 17 terminates within the housinq 1 in an oxygen distribution device.
The extraction of the oxygen ~rom the inner space o~ the housing 1 takes place above a specific predeterminable pressure via a pipe conduit or a hose z~ which is closed b~low this presetta~le pressure by a pressure relief valve ,.,-"' , . "' ;:. : .
2 ~ 6 ~
24. The end of the pipe conduit 23 arranged within the housing 1 is turned back in an arc shape to the floor of the housing l in order to prevent the penetration of water or contamination.
This apparatus operates in the following way when carrying out the method or the invention:
After a tissue and organ to be treated has been laid into the housing l the housing door 4 is closed and locked. The control unit 8 now causes the filling of a non-illustrated pneumatic seal in the form of a hose which is laid in a closed path around the opening ~ with air at high press~re, whereby the diameter of the hose is ~roadened in order to seal off the doors 4 relative to ~he housing 1 in a gas and pressure tight manner.
In this initial state the so'enoid valves 13, 16, 18, Z0 and 21 are closed.
Thereafter the inner space of ths housing is evacuated for approximately one hour, with the colenoid valve 13 being opened via the control unit 8. At the end of the one hour evacuation time a vacuum has almost been achieved within the housing 1, i.e. a depression of 50 to 100 mbar prevails Approximately two hours before the start of the evacuation of the housing l the generation o~ oxygen has already started via the oxygen genera.tor 9, with the solenoid valves 18 and 22 being opened, the soienoid valve 20, however, still remains closed.
The oxygen thus flows after corresponding cleaning via a heat exchanger 19 and th~ pipe conduit 21 into t~e oxygen tank 7 in which an excess pressure of approxi~ately 6 bar 218h~.~4 prevails at the end of the one hour evacuation period.
After approximately one hour the evacuation ~ the interior space o~ the housing is terminated and the solenoid valve 13 is closed. The solenoid valve ZO is now opened so that the oxygen standing under excess pressure can ~low ~rom the oxygen tank 7 into the inner space of the housing 1. After approximately five mi~utes a pressure equalisation takes place between the oxygen tank 7 and the inner space o~ the hcusing 1 and lies approximately at O to C.6 bar excess pressure.
A~ter the oxygen at~osphere with~n the hous~ng 1 has been built up relatively rapidly, the solenoid valve 22 closes with pressure equalisation so that the oxygen ~rom the oxygen generator 5 is directed via a direct route into the interior of the housin~.
After a several hour long build up phase an oxyg~n pressure of approximately 10 to 11 bar has been achieved in t~e interior space of the housing, at which the pressure relief valve 24 open~. From this point in time onward the oxygen flows continuously ~rom the area o~ the inlet opening lO to the discharge opening 11 so that the interior space of the housing is continuously flooded with fresh oxygen. This state is reta~ned until the end of the intermediate storage time which can last from ~everal hours up to several days.
~ter the termination o~ ~he desired period of intermediate storage the oxygen generator S is switched off and the two solenoid ~alves la and 20 are closed. The solenoid valve 1 is opened for a ~ew minutes in order to achieve a pressure equalisation between the interior space of the housing and the ambient atmosphere.
21~2~64 Prior to opening the door 4 of the housing the air is necessarily let out of the pneumatic door seal ~ia a correspondingly controlled solenoid valve in order to avoid the door seal being damaged through the missing counter-pressure on opening the door.
The build-up of the oxygen atmosphere can take place in the method of the invention after the ga~ corresponding to the environmental or ambient atmosphe~e has been removed ~rom the closeable space in a preceding step. It is, however, also possible to blow oxygen directly with excess pressure into the space and to thereby displace the ga~ corresponding to the environmental atmosphere out of the space. It is important that an oxygen atmosphere is provided in some way or another in the closa~le space with a pressure which is elevated relative to the environment.
In this respect the oxygen atmosphere provided in the space should advantageously have a degree of purity of at least 50 ~, in particular of more than 80 % and prefera~ly of at least 93 %, whereby a situation is achieved in which an extre~ely pronounced preser~ation effect results for the treated tissue.
The method of the inven~ion can be carried out particularly effectively when the evacuation process is carrled out over a time interval of one hour, with the pressure within the closed space sinking to a depression of 50 to loO mbar, and if the build-up o~ the oxygen atmosphere within the closed space then takes place to a pre~sure o~ 6 to 11 bar.
During the period of intermediate storage a continuous or di~continuous supplementation of the oxygen atmosphere in the closed space can be effected, ;~ith oxygen preferably being continuously introduced into the closed space at a pressure which is higher than a predeterminable discharge pressure so that a continuous through-flow sets in as a result of the pressure di~ference. Through this preferably continuous supplementatlon of the oxygen in the closed-off ?
2~82164 The invention relates to a method of preserving tissues and organs, in particular of tissues and organ~ for transplan-tation.
With tissues and organs for transplantation the problem always exists that the transplantation must take place within a very short time, generally within a few hours after the removal of the tissue or organ from a body. An attempt is admittedly made to extend the time interval in which the tissue or organs can be transplanted into a new body by main-taining specific temperatures. However, this is only possible to an inadequate degree.
It is the object of the present invention to set ~orth a method by which tiSSIle and organs, in particular tissues and organs for transplantatlon, can ~e preserved over a long period of time without their cell structure changing.
This o~ject i~ satisfied in accordance with the invention essentially by a method in which the organs or tissues are te~porarily stored in a well-cooled state, in particular at a temperature in the range from -~C to 3~C, for a predeter-minable time in an oxygeli atmosphere, in a space which can be closed of~ in air tight ~anner, wherein the oxygen atmo-sphere is built up in the ~pace which can be sealed o~f in air tight manner with a pressure which lies above the atmo-spheric pressure, and wherein the pressure in the closed space is kept at a pressure lying a~ove the atmospheric pressure for the whole of the ~urther period of intermediate storage.
It has surprisingly been ~ound that tissues and organs which are treated by this method do not have any changes in their ; ' ~ , space the original oxygen content in the space can be maintained during the entire period of intermediate storage so that a particularly good preservation effect ~ets in.
Further advantageous embodiments of the invention are evident from the subordinate claims.
The in~ention will be explained in more detail by way o~
example in the followlng with reference to the drawinas in which are shown.
Figure 1 a schematic side-view o~ an apparatus ~or carrying - out the method o~ the invention, and Figure 2 a schematic illustration to ~a~e clear the ~anner of operation of the apparatus for carryinq the method o~ the invention.
~igure 1 shows an apparatus in accordance with the inven-tion, ~ith a housing of closed design consisting of a welded construction, having an opening 3 in order to place tissue or organs to be treated into the housing 1 in a manner such that oxygen has access to them from substantially all sides.
The opening 3 can be sealingly closed o~f by means of the housing door 4.
An oxygen generator 5, an evacuation pump 6 and also an oxyqen tank 7 and an f.~lectronic control unit 8 are mounted on the housing l The housing 1 furthermore has, as shown schematically in Figure 2, an evacuation opening 9 and an oxygen inlet opening 10 at the roof side and also an oxygen extraction opening 11 at the floor side.
.
.. .
2~ 8~1 64 The evacua~ion pump 6 is connected to the evacuation opening 9 via a pipe conduit or hose 14 closable by means of a solenoid valve 13.
A pipe conduit or a hose 15, which can likewise be closed by means of a solenoid valve 16, branc~es off fro~ the pipe conduit 14 between the solenoid valve 13 and the evacuation opening 9 and opens lnto the environmental atmosphPre.
The oxygen generator 5 is con~ected via a pipe conduit or a hose 17 to the inle~ opening 10, with the suction end of the pipe conduit 14 being closable by means of a solenoid valve 18.
A heat exchanger 19 in the form of a Peltier ele~ent is furthermore provided between the oxygen generator 5 and the inlet opening lO. Here the temperature of the oxygen ls measured and the oxygen is cooled down to ca. 0C. The cooling of the oxygen contribu~es significantly to the preservation of the organs and tissues.
A solenoid valve 20 is further~ore provided ~etween the oxygen generator 5 or the ~eat exc~anger 19 and the inlet opening lO.
A further pipe conduit or a hose 21, which can be closed by a solenoid valve 22 an~ leads to ~he oxygen tank 7, branches off from the pipe conduit 17 between the solenoid valve Z~
and the heat exchanger 19. The pipe ccnduit 17 terminates within the housinq 1 in an oxygen distribution device.
The extraction of the oxygen ~rom the inner space o~ the housing 1 takes place above a specific predeterminable pressure via a pipe conduit or a hose z~ which is closed b~low this presetta~le pressure by a pressure relief valve ,.,-"' , . "' ;:. : .
2 ~ 6 ~
24. The end of the pipe conduit 23 arranged within the housing 1 is turned back in an arc shape to the floor of the housing l in order to prevent the penetration of water or contamination.
This apparatus operates in the following way when carrying out the method or the invention:
After a tissue and organ to be treated has been laid into the housing l the housing door 4 is closed and locked. The control unit 8 now causes the filling of a non-illustrated pneumatic seal in the form of a hose which is laid in a closed path around the opening ~ with air at high press~re, whereby the diameter of the hose is ~roadened in order to seal off the doors 4 relative to ~he housing 1 in a gas and pressure tight manner.
In this initial state the so'enoid valves 13, 16, 18, Z0 and 21 are closed.
Thereafter the inner space of ths housing is evacuated for approximately one hour, with the colenoid valve 13 being opened via the control unit 8. At the end of the one hour evacuation time a vacuum has almost been achieved within the housing 1, i.e. a depression of 50 to 100 mbar prevails Approximately two hours before the start of the evacuation of the housing l the generation o~ oxygen has already started via the oxygen genera.tor 9, with the solenoid valves 18 and 22 being opened, the soienoid valve 20, however, still remains closed.
The oxygen thus flows after corresponding cleaning via a heat exchanger 19 and th~ pipe conduit 21 into t~e oxygen tank 7 in which an excess pressure of approxi~ately 6 bar 218h~.~4 prevails at the end of the one hour evacuation period.
After approximately one hour the evacuation ~ the interior space o~ the housing is terminated and the solenoid valve 13 is closed. The solenoid valve ZO is now opened so that the oxygen standing under excess pressure can ~low ~rom the oxygen tank 7 into the inner space of the housing 1. After approximately five mi~utes a pressure equalisation takes place between the oxygen tank 7 and the inner space o~ the hcusing 1 and lies approximately at O to C.6 bar excess pressure.
A~ter the oxygen at~osphere with~n the hous~ng 1 has been built up relatively rapidly, the solenoid valve 22 closes with pressure equalisation so that the oxygen ~rom the oxygen generator 5 is directed via a direct route into the interior of the housin~.
After a several hour long build up phase an oxyg~n pressure of approximately 10 to 11 bar has been achieved in t~e interior space of the housing, at which the pressure relief valve 24 open~. From this point in time onward the oxygen flows continuously ~rom the area o~ the inlet opening lO to the discharge opening 11 so that the interior space of the housing is continuously flooded with fresh oxygen. This state is reta~ned until the end of the intermediate storage time which can last from ~everal hours up to several days.
~ter the termination o~ ~he desired period of intermediate storage the oxygen generator S is switched off and the two solenoid ~alves la and 20 are closed. The solenoid valve 1 is opened for a ~ew minutes in order to achieve a pressure equalisation between the interior space of the housing and the ambient atmosphere.
21~2~64 Prior to opening the door 4 of the housing the air is necessarily let out of the pneumatic door seal ~ia a correspondingly controlled solenoid valve in order to avoid the door seal being damaged through the missing counter-pressure on opening the door.
Claims (13)
1. Method of preserving tissues and organs, in particular tissues and organs for transplantation in which the tissue and the organs are temporarily stored in a well-cooled state, in particular at a temperature in the range from -3°C to 3°C, for a predeterminable time in an oxygen atmosphere, in a space which can be closed off in air tight manner, wherein the oxygen atmosphere is built up in the space which can be sealed off in air tight manner with a pressure which lies above the atmospheric pressure, and wherein the pressure in the closed space is kept at a pressure lying above the atmospheric pressure for the whole of the further period of inter-mediate storage.
2. Method in accordance with claim 1, characterised in that the oxygen atmosphere is built up after extracting the gas corresponding to the environmental atmosphere from the closable space.
3. Method in accordance with claim 1, characterised in that the oxygen atmosphere is provided by blowing oxygen stan-ding under elevated pressure into the closed-off space.
4. Method in accordance with claim 1, characterised in that the oxygen atmosphere has a degree of purity of at least 50 %, in particular of more than 80 % and preferably of at least 93 %.
5. Method in accordance with claim 1, characterised in that the evacuation procedure is carried out during a time interval of at least minutes and up to six hours, in particular from one to three hours and preferably of one hour, with the pressure in the closed-off space being reduced to a pressure which lies substantially beneath the atmospheric pressure, in particular to a depression relative to atmospheric pressure of approximately 50 to 100 mbar.
6. Method in accordance with claim 1, characterised in that the evacuation process is effected with a steeper pressure reduction gradient in the first phase of the evacuation process than in the last phase of the evacua-tion process.
7. Method in accordance with claim 1, characterised in that oxygen is generated before and/or during the evacuation process and is stored outside of the closed space in an oxygen tank at excess pressure, preferably with 6 bar excess pressure relative to the atmospheric pressure;
and in that at least the initial build-up of the oxygen atmosphere in the closed-off space is carried out after the end of the evacuation process in an accelerated manner by means of the stored oxygen.
and in that at least the initial build-up of the oxygen atmosphere in the closed-off space is carried out after the end of the evacuation process in an accelerated manner by means of the stored oxygen.
8. Method in accordance with claim 1, characterised in that after the pressure equalisation between the oxygen tank and the closed-off space the connection between the oxy-gen tank and the closed-off space is interrupted and the oxygen required for the further build-up of the excess pressure is supplied to the closed-off space directly from the oxygen source.
9. Method in accordance with claim 8, characterised in that the pressure equalisation takes place at approximately 0 to 0.6 bar.
10. Method in accordance with claim 8, characterised in that the oxygen is supplied to the closed-off space from the oxygen source at a pressure which is somewhat higher than the pressure to be achieved in the closed-off space.
11. Method in accordance with claim 1, characterised in that the oxygen atmosphere in the closed-off space is brought to and kept at an excess pressure relative to the atmo-spheric pressure of 1 to 20 bar, and preferably of 6 to 11 bar during the period of intermediate storage.
12. Method in accordance with claim 1, characterised in that during the period of intermediate storage a continuous or discontinuous supplementation of the oxygen atmo-sphere in the closed space is effected, with oxygen preferably being continuously introduced into the closed space at a pressure which is higher than a predetermi-nable discharge pressure so that a continuous through-flow sets in as a result of the pressure difference.
13. Method in accordance with claim 1, characterised in that the period of intermediate storage lies in the range from several hours to several days.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19527734A DE19527734A1 (en) | 1995-07-28 | 1995-07-28 | Method and device for preserving tissues and organs, in particular transplant tissues and organs |
| DE19527734 | 1995-07-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2182164A1 true CA2182164A1 (en) | 1997-01-29 |
Family
ID=7768099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002182164A Abandoned CA2182164A1 (en) | 1995-07-28 | 1996-07-26 | Method of preserving tissues and organs |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0755625A1 (en) |
| JP (1) | JPH09104601A (en) |
| CA (1) | CA2182164A1 (en) |
| DE (1) | DE19527734A1 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6140123A (en) * | 1998-10-07 | 2000-10-31 | Cedars-Sinai Medical Center | Method for conditioning and cryopreserving cells |
| DE19915883A1 (en) * | 1999-04-08 | 2000-10-19 | Solich Gmbh | Process and device for the treatment of food, in particular meat, sausage and fish |
| DE19922310B4 (en) * | 1999-05-14 | 2006-01-19 | Roger Berg | Pressure-resistant container for the preservation of tissues and organs |
| JP6988286B2 (en) * | 2017-09-08 | 2022-01-05 | 株式会社ジェイ・エム・エス | Transport device for organs and biological tissues |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3468136A (en) * | 1964-08-25 | 1969-09-23 | Emil S Swenson | Method for maintaining organs in a completely viable state |
| US3772153A (en) * | 1969-04-02 | 1973-11-13 | Air Liquide | Apparatus for the preservation of animal or human organs in living condition |
| SU594984A1 (en) * | 1976-06-22 | 1978-02-28 | Второй Московский Ордена Ленина Государственный Медицинский Институт Им. Н.И.Пирогова | Kidney preservation method |
| US4837390A (en) * | 1983-05-11 | 1989-06-06 | Keyes Offshore, Inc. | Hyperbaric organ preservation apparatus and method for preserving living organs |
| JPS60197601A (en) * | 1984-03-16 | 1985-10-07 | Yasuhiro Fukui | Storage of excised organs and installation therefor |
| DE3581407D1 (en) * | 1985-09-06 | 1991-02-21 | Nestle Sa | STORAGE OF LIVING TISSUE. |
| US4879283A (en) * | 1985-10-03 | 1989-11-07 | Wisconsin Alumni Research Foundation | Solution for the preservation of organs |
| DE8533481U1 (en) * | 1985-11-28 | 1986-05-22 | Fresenius AG, 6380 Bad Homburg | Perfusion device |
| EP0347923A1 (en) * | 1988-06-23 | 1989-12-27 | Nicholas J. Perrotta | Portable pulsatile organ perfusion device and method |
| US5326706A (en) * | 1989-07-17 | 1994-07-05 | Research Foundation Of State University Of New York | Homeostatic organ preservation system |
| ES2093648T3 (en) * | 1989-07-27 | 1997-01-01 | Leonora I Jost | BIOLOGICAL SUPPORT SYSTEM, CONTAINER AND PROCEDURE FOR PRESERVING LIVING MAMMALS, EX VIVO. |
| EP0440925A1 (en) * | 1989-12-13 | 1991-08-14 | The Green Cross Corporation | Internal organ-preserving liquid and method for preserving internal organs |
| US5395314A (en) * | 1990-10-10 | 1995-03-07 | Life Resuscitation Technologies, Inc. | Brain resuscitation and organ preservation device and method for performing the same |
| DE59106737D1 (en) * | 1990-12-20 | 1995-11-23 | Ines Ag | METHOD AND DEVICE FOR TREATING FRESH MEAT. |
| US5217860A (en) * | 1991-07-08 | 1993-06-08 | The American National Red Cross | Method for preserving organs for transplantation by vitrification |
| DE4201259C2 (en) * | 1992-01-18 | 1994-12-08 | Sachs Elektronik Kg Hugo | Device for perfusion of the coronary arteries of an isolated heart |
| DE4201258A1 (en) * | 1992-01-18 | 1993-07-22 | Sachs Elektronik Kg Hugo | PERFUSION DEVICE |
| BE1007500A3 (en) * | 1992-09-18 | 1995-07-18 | Pasteur Merieux Serums Vacc | Solution infusion, conservation and organ reperfusion. |
| US5338662A (en) * | 1992-09-21 | 1994-08-16 | Bio-Preserve Medical Corporation | Organ perfusion device |
| US5362622A (en) * | 1993-03-11 | 1994-11-08 | Board Of Regents, The University Of Texas System | Combined perfusion and oxygenation apparatus |
| US5356771A (en) * | 1993-03-11 | 1994-10-18 | Board Of Regents, The University Of Texas System | Combined perfusion and oxygenation organ preservation apparatus |
| DE4407863C2 (en) * | 1993-06-07 | 1997-08-07 | Mayer Berndt Priv Doz Dr Med | Method and device for the preservation of organs, extremities and tissue flaps |
| DE4415184A1 (en) * | 1993-10-22 | 1995-04-27 | Hubert Verhaag | Process and apparatus for treating fresh meat and plants |
| DE4342728A1 (en) * | 1993-12-15 | 1995-06-22 | Thomae Gmbh Dr K | Aq. isotonic soln. for perfusion and preservation of explanted liver |
-
1995
- 1995-07-28 DE DE19527734A patent/DE19527734A1/en not_active Withdrawn
-
1996
- 1996-07-25 EP EP96112022A patent/EP0755625A1/en not_active Withdrawn
- 1996-07-26 JP JP8227294A patent/JPH09104601A/en active Pending
- 1996-07-26 CA CA002182164A patent/CA2182164A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JPH09104601A (en) | 1997-04-22 |
| EP0755625A1 (en) | 1997-01-29 |
| DE19527734A1 (en) | 1997-01-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20040726 |