CA2143831A1 - Polymeres hydrosolubles pour immunoessais et essais d'hybridation de l'adn - Google Patents
Polymeres hydrosolubles pour immunoessais et essais d'hybridation de l'adnInfo
- Publication number
- CA2143831A1 CA2143831A1 CA 2143831 CA2143831A CA2143831A1 CA 2143831 A1 CA2143831 A1 CA 2143831A1 CA 2143831 CA2143831 CA 2143831 CA 2143831 A CA2143831 A CA 2143831A CA 2143831 A1 CA2143831 A1 CA 2143831A1
- Authority
- CA
- Canada
- Prior art keywords
- specific binding
- polymer
- receptor
- solid phase
- indicator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000003556 assay Methods 0.000 title abstract description 47
- 238000003018 immunoassay Methods 0.000 title abstract description 16
- 229920003169 water-soluble polymer Polymers 0.000 title description 11
- 238000009396 hybridization Methods 0.000 title description 10
- 229920000642 polymer Polymers 0.000 claims abstract description 71
- 230000009870 specific binding Effects 0.000 claims abstract description 55
- 238000000034 method Methods 0.000 claims abstract description 40
- 230000027455 binding Effects 0.000 claims abstract description 39
- 239000007864 aqueous solution Substances 0.000 claims abstract description 11
- 239000007790 solid phase Substances 0.000 claims description 66
- 238000006243 chemical reaction Methods 0.000 claims description 59
- 239000012491 analyte Substances 0.000 claims description 46
- 238000000159 protein binding assay Methods 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000005859 coupling reaction Methods 0.000 claims description 18
- 230000001900 immune effect Effects 0.000 claims description 17
- 239000003365 glass fiber Substances 0.000 claims description 16
- 230000008878 coupling Effects 0.000 claims description 15
- 238000010168 coupling process Methods 0.000 claims description 15
- 239000002904 solvent Substances 0.000 claims description 13
- 239000011541 reaction mixture Substances 0.000 claims description 8
- 150000008064 anhydrides Chemical class 0.000 claims description 6
- 239000000427 antigen Substances 0.000 claims description 5
- 102000036639 antigens Human genes 0.000 claims description 5
- 108091007433 antigens Proteins 0.000 claims description 5
- 239000012634 fragment Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 4
- 102000014914 Carrier Proteins Human genes 0.000 claims description 3
- 229920002873 Polyethylenimine Polymers 0.000 claims description 3
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 3
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 3
- 230000003100 immobilizing effect Effects 0.000 claims description 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 2
- 108091008324 binding proteins Proteins 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 18
- 239000000203 mixture Substances 0.000 abstract description 8
- 239000002245 particle Substances 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000000523 sample Substances 0.000 description 32
- 239000000376 reactant Substances 0.000 description 27
- 108020004414 DNA Proteins 0.000 description 23
- 239000000243 solution Substances 0.000 description 23
- 102000004169 proteins and genes Human genes 0.000 description 15
- 108090000623 proteins and genes Proteins 0.000 description 15
- 239000000463 material Substances 0.000 description 13
- 239000011159 matrix material Substances 0.000 description 13
- 239000012530 fluid Substances 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- 239000000872 buffer Substances 0.000 description 10
- 238000010790 dilution Methods 0.000 description 10
- 239000012895 dilution Substances 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 10
- 238000000926 separation method Methods 0.000 description 10
- 239000007787 solid Substances 0.000 description 10
- 239000011859 microparticle Substances 0.000 description 9
- 239000000758 substrate Substances 0.000 description 7
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 6
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 239000007983 Tris buffer Substances 0.000 description 5
- 230000004071 biological effect Effects 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 239000004094 surface-active agent Substances 0.000 description 5
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 4
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 230000009871 nonspecific binding Effects 0.000 description 4
- 239000011148 porous material Substances 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 238000005192 partition Methods 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000012064 sodium phosphate buffer Substances 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- BCHIXGBGRHLSBE-UHFFFAOYSA-N (4-methyl-2-oxochromen-7-yl) dihydrogen phosphate Chemical compound C1=C(OP(O)(O)=O)C=CC2=C1OC(=O)C=C2C BCHIXGBGRHLSBE-UHFFFAOYSA-N 0.000 description 2
- 101710132601 Capsid protein Proteins 0.000 description 2
- 108010078791 Carrier Proteins Proteins 0.000 description 2
- 102000006395 Globulins Human genes 0.000 description 2
- 108010044091 Globulins Proteins 0.000 description 2
- -1 Intrinsic Factor Proteins 0.000 description 2
- 102000057297 Pepsin A Human genes 0.000 description 2
- 108090000284 Pepsin A Proteins 0.000 description 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
- 101001039269 Rattus norvegicus Glycine N-methyltransferase Proteins 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 2
- 239000013068 control sample Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- YYXLGGIKSIZHSF-UHFFFAOYSA-N ethene;furan-2,5-dione Chemical compound C=C.O=C1OC(=O)C=C1 YYXLGGIKSIZHSF-UHFFFAOYSA-N 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 229940029329 intrinsic factor Drugs 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000013610 patient sample Substances 0.000 description 2
- 229940111202 pepsin Drugs 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000012209 synthetic fiber Substances 0.000 description 2
- 229920002994 synthetic fiber Polymers 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000001685 thyroid gland Anatomy 0.000 description 2
- BQWBEDSJTMWJAE-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-[(2-iodoacetyl)amino]benzoate Chemical compound C1=CC(NC(=O)CI)=CC=C1C(=O)ON1C(=O)CCC1=O BQWBEDSJTMWJAE-UHFFFAOYSA-N 0.000 description 1
- MRAKLTZPBIBWFH-ARJAWSKDSA-N (z)-2-ethenylbut-2-enedioic acid Chemical compound OC(=O)\C=C(\C=C)C(O)=O MRAKLTZPBIBWFH-ARJAWSKDSA-N 0.000 description 1
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical group Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 1
- 108091005508 Acid proteases Proteins 0.000 description 1
- FBEHFRAORPEGFH-UHFFFAOYSA-N Allyxycarb Chemical compound CNC(=O)OC1=CC(C)=C(N(CC=C)CC=C)C(C)=C1 FBEHFRAORPEGFH-UHFFFAOYSA-N 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000006177 biological buffer Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 150000001793 charged compounds Chemical class 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 238000012875 competitive assay Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000007822 coupling agent Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 229920005684 linear copolymer Polymers 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000005291 magnetic effect Effects 0.000 description 1
- FPYJFEHAWHCUMM-UHFFFAOYSA-N maleic anhydride Chemical compound O=C1OC(=O)C=C1 FPYJFEHAWHCUMM-UHFFFAOYSA-N 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 108700010839 phage proteins Proteins 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Ces procédés et composés sont destinés aux dosages immunologiques dans lesquels un récepteur de liaison spécifique est couplé à un plymère dont le squelette est linéaire. Le complexe récepteur: polymère résultant est soluble dans une solution aqueuse. Ce complexe présente l'avantage de permettre l'exécution des dosages sans recourir à des réactifs de liaison secondaires ou sans recourir à des particules.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8828493A | 1993-07-06 | 1993-07-06 | |
| US08/088,284 | 1993-07-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2143831A1 true CA2143831A1 (fr) | 1995-01-19 |
Family
ID=22210481
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2143831 Abandoned CA2143831A1 (fr) | 1993-07-06 | 1994-07-06 | Polymeres hydrosolubles pour immunoessais et essais d'hybridation de l'adn |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0658251A1 (fr) |
| JP (1) | JPH08506902A (fr) |
| AU (1) | AU7255594A (fr) |
| CA (1) | CA2143831A1 (fr) |
| WO (1) | WO1995002184A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6150103A (en) * | 1997-07-22 | 2000-11-21 | Qiagen Genomics, Inc. | Polyethylenimine-based biomolecule arrays |
| EP0996500A1 (fr) | 1997-07-22 | 2000-05-03 | Rapigene, Inc. | Appareil et procedes pour realiser une dispersion matricielle d'une solution sur un support solide |
| US8048659B1 (en) * | 1999-10-08 | 2011-11-01 | Leif Robert C | Conjugated polymer tag complexes |
| JP2004286728A (ja) * | 2002-07-09 | 2004-10-14 | Sumitomo Bakelite Co Ltd | 水性組成物、水性液および生理活性物質の固定化方法 |
| US8404621B2 (en) | 2008-03-24 | 2013-03-26 | Fujifilm Corporation | Method for immobilization, physiologically active substance-immobilized carrier, carrier for immobilization, carrier, and process for producing carrier |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MC1637A1 (fr) * | 1983-11-10 | 1986-01-09 | Genetic Systems Corp | Composes polymerisables contenant des polypeptides comme partie integrante et leur utilisation dans des epreuves immunologiques de separation induite par polymerisation |
-
1994
- 1994-07-06 JP JP7504126A patent/JPH08506902A/ja active Pending
- 1994-07-06 WO PCT/US1994/007549 patent/WO1995002184A1/fr not_active Application Discontinuation
- 1994-07-06 AU AU72555/94A patent/AU7255594A/en not_active Abandoned
- 1994-07-06 CA CA 2143831 patent/CA2143831A1/fr not_active Abandoned
- 1994-07-06 EP EP94922092A patent/EP0658251A1/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JPH08506902A (ja) | 1996-07-23 |
| EP0658251A1 (fr) | 1995-06-21 |
| AU7255594A (en) | 1995-02-06 |
| WO1995002184A1 (fr) | 1995-01-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |