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CA2093927A1 - Methode immunofonctionnelle de dosage a mediation par un ligand d'une proteine liant une hormone - Google Patents

Methode immunofonctionnelle de dosage a mediation par un ligand d'une proteine liant une hormone

Info

Publication number
CA2093927A1
CA2093927A1 CA2093927A CA2093927A CA2093927A1 CA 2093927 A1 CA2093927 A1 CA 2093927A1 CA 2093927 A CA2093927 A CA 2093927A CA 2093927 A CA2093927 A CA 2093927A CA 2093927 A1 CA2093927 A1 CA 2093927A1
Authority
CA
Canada
Prior art keywords
binding protein
hormone
ligand
bound
growth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2093927A
Other languages
English (en)
Other versions
CA2093927C (fr
Inventor
Lena M. S. Carlsson
Ross G. Clark
Wai L. T. Wong
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA002291983A priority Critical patent/CA2291983C/fr
Publication of CA2093927A1 publication Critical patent/CA2093927A1/fr
Application granted granted Critical
Publication of CA2093927C publication Critical patent/CA2093927C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2869—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against hormone receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/61—Growth hormones [GH] (Somatotropin)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Neurology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Rheumatology (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Orthopedic Medicine & Surgery (AREA)

Abstract

Procédé de dosage immunofonctionnel induit par ligands (LIFA) permettant de détecter la présence et la concentration de protéines de liaison d'hormones polypeptidiques consistant à capturer la protéine de liaison à l'aide d'un premier anticorps lié à une phase solide, à saturer la protéine de liaison d'hormones avec l'hormone polypeptidique de ligand, et à détecter l'hormone polypeptidique de ligands liée à l'aide d'un second anticorps marqué de manière détectable et spécifique de l'hormone polypeptidique de ligands. En l'absence d'hormone polypeptidique de saturation ajoutée, le LIFA mesure la quantité de protéines de liaison d'hormones liées à l'hormone polypeptidique de ligands endogènes . Un dosage de protéines de liaison d'hormones de croissance illustre le procédé de l'invention. Les résultats du dosage LIFA indiquent qu'une protéine de liaison accrue augmente sensiblement l'activité de l'hormone de croissance. L'invention concerne également des procédés d'utilisation ainsi que des formulations de protéine de liaison d'hormone de croissance, d'hormone de croissance, de facteur-I de croissance analogue à l'insuline et de protéine de liaison de facteur de croissance analogue à l'insuline.
CA002093927A 1990-11-19 1991-11-19 Methode immunofonctionnelle de dosage a mediation par un ligand d'une proteine liant une hormone Expired - Lifetime CA2093927C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002291983A CA2291983C (fr) 1991-11-19 1991-11-19 Methode immunofonctionnelle de dosage a mediation par un ligand d'une proteine liant une hormone

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/615,538 1990-11-19
US07/615,538 US5210017A (en) 1990-11-19 1990-11-19 Ligand-mediated immunofunctional hormone binding protein assay method
PCT/US1991/008664 WO1992008985A2 (fr) 1990-11-19 1991-11-19 Procede de dosage de proteines de liaison d'hormones immunofonctionnel induit par ligands

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002291983A Division CA2291983C (fr) 1991-11-19 1991-11-19 Methode immunofonctionnelle de dosage a mediation par un ligand d'une proteine liant une hormone

Publications (2)

Publication Number Publication Date
CA2093927A1 true CA2093927A1 (fr) 1992-05-20
CA2093927C CA2093927C (fr) 2000-07-18

Family

ID=24465822

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002093927A Expired - Lifetime CA2093927C (fr) 1990-11-19 1991-11-19 Methode immunofonctionnelle de dosage a mediation par un ligand d'une proteine liant une hormone

Country Status (12)

Country Link
US (1) US5210017A (fr)
EP (3) EP0681842B1 (fr)
JP (2) JP2977280B2 (fr)
AT (3) ATE138739T1 (fr)
AU (1) AU657109B2 (fr)
CA (1) CA2093927C (fr)
DE (3) DE69119911T2 (fr)
DK (3) DK0558647T3 (fr)
ES (3) ES2171478T3 (fr)
GR (1) GR3020505T3 (fr)
HK (1) HK1007351A1 (fr)
WO (1) WO1992008985A2 (fr)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866350A (en) * 1985-03-19 1999-02-02 Helen Hwai-An Lee Method for the immunological determination of a biological material in a sample
US5981485A (en) 1997-07-14 1999-11-09 Genentech, Inc. Human growth hormone aqueous formulation
US5593844A (en) * 1990-11-19 1997-01-14 Genentech, Inc. Ligand-mediated immunofunctional hormone binding protein assay method
US6326154B1 (en) * 1990-11-19 2001-12-04 Genentech, Inc. Ligand-mediated immunofunctional hormone binding protein assay method
US5464757A (en) * 1991-01-15 1995-11-07 The Salk Institute For Biological Studies DNA encoding CRF binding protein
DE69334134T2 (de) * 1992-07-31 2007-12-20 Genentech, Inc., South San Francisco Wässrige Formulierung enthaltend Menschliches Wachstumshormon
US6087188A (en) * 1992-11-13 2000-07-11 Alk A/S Two-site immunoassay for an antibody with chemiluminescent label and biotin bound ligand
US5981252A (en) * 1993-06-25 1999-11-09 Smithkline Beecham Lipoprotein associated phospholipase A2, inhibitors thereof and use of the same in diagnosis and therapy
US6267958B1 (en) 1995-07-27 2001-07-31 Genentech, Inc. Protein formulation
AUPN801296A0 (en) * 1996-02-12 1996-03-07 Csl Limited Stabilised growth hormone formulation and method of preparation thereof
US6121416A (en) 1997-04-04 2000-09-19 Genentech, Inc. Insulin-like growth factor agonist molecules
US6420518B1 (en) 1997-04-04 2002-07-16 Genetech, Inc. Insulin-like growth factor agonist molecules
ES2234560T3 (es) * 1999-01-06 2005-07-01 Genentech, Inc. Variante mutante del factor de crecimiento de tipo insulina (igf-i).
DE60133271T2 (de) * 2000-05-16 2009-04-23 Genentech, Inc., South San Francisco Behandlung von knorpelerkrankungen
US7108993B2 (en) * 2002-07-19 2006-09-19 Bayer Healthcare Llc Use of dual conjugated labels in the elimination of serum interference in immunochromatographic assays
SE0300959D0 (sv) * 2002-12-19 2003-04-02 Pharmacia Ab Methods for predicting therapeutic response to agents acting on the growth hormone receptor
US7920906B2 (en) 2005-03-10 2011-04-05 Dexcom, Inc. System and methods for processing analyte sensor data for sensor calibration
HUE027218T2 (en) 2003-09-12 2016-10-28 Ipsen Biopharmaceuticals Inc Methods for Treatment of IGF-1 (Insulin-like Growth Factor 1) Deficiency
US9247900B2 (en) 2004-07-13 2016-02-02 Dexcom, Inc. Analyte sensor
WO2006127694A2 (fr) 2004-07-13 2006-11-30 Dexcom, Inc. Detecteur d'analyte
US7654956B2 (en) 2004-07-13 2010-02-02 Dexcom, Inc. Transcutaneous analyte sensor

Family Cites Families (17)

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DE263537C (fr) *
NL7501215A (nl) * 1975-02-01 1976-08-03 Akzo Nv Methode voor het aantonen en bepalen van een antigeen of antilichaam.
US4486530A (en) * 1980-08-04 1984-12-04 Hybritech Incorporated Immunometric assays using monoclonal antibodies
US4476228A (en) * 1982-11-08 1984-10-09 Abbott Laboratories Determination of unsaturated thyroxine binding protein sites using fluorescence polarization techniques
US4622293A (en) * 1983-07-05 1986-11-11 Miles Laboratories, Inc. Iodothyronine immunoassays employing HMS as TBP blocking agent
US4594327A (en) * 1983-11-02 1986-06-10 Syntex (U.S.A.) Inc. Assay method for whole blood samples
IL74201A0 (en) * 1984-01-30 1985-04-30 Icrf Patents Limited Polypeptides for the detection and control of mammalian cell growth
US4737456A (en) * 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
DD263537A1 (de) * 1987-09-01 1989-01-04 Akad Wissenschaften Ddr Verfahren zur herstellung von monoklonalen antikoerpern gegen humanes wachstumshormon
US4956302A (en) * 1987-09-11 1990-09-11 Abbott Laboratories Lateral flow chromatographic binding assay device
EP0404783A4 (en) * 1988-01-28 1991-01-23 E.I. Du Pont De Nemours And Company Immunoassays using monoclonal antibodies directed against natural binding proteins
DK131988A (da) * 1988-03-11 1989-09-12 Erasmus University Igf-bindingsprotein, dna-struktur, der koder for igf-bindingsproteinet og vektor indeholdende denne dna-struktur
AU1955388A (en) * 1988-04-12 1989-11-03 Synergen, Inc. Method for potentiating and inhibiting insulin-like growth factor activity
WO1990000569A1 (fr) * 1988-07-15 1990-01-25 Central Sydney Area Health Service Sous-unite labile aux acides d'un complexe proteique de liaison du facteur de croissance analogue a l'insuline
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Also Published As

Publication number Publication date
EP0681842B1 (fr) 2002-02-13
ATE138739T1 (de) 1996-06-15
JPH06502724A (ja) 1994-03-24
DK0681842T3 (da) 2002-06-03
EP0995445A3 (fr) 2004-01-07
CA2093927C (fr) 2000-07-18
JP2977280B2 (ja) 1999-11-15
WO1992008985A3 (fr) 1992-10-29
JP3159966B2 (ja) 2001-04-23
HK1007351A1 (en) 1999-04-09
EP0995445A2 (fr) 2000-04-26
EP0995445B1 (fr) 2009-03-18
DE69133614D1 (de) 2009-04-30
EP0558647A1 (fr) 1993-09-08
DK0558647T3 (da) 1996-08-19
ES2090598T3 (es) 1996-10-16
DE69119911T2 (de) 1996-11-21
JPH11346765A (ja) 1999-12-21
DE69132931T2 (de) 2002-11-28
EP0681842A3 (fr) 1998-06-10
ES2171478T3 (es) 2002-09-16
DE69119911D1 (de) 1996-07-04
AU9106791A (en) 1992-06-11
ES2324322T3 (es) 2009-08-04
EP0681842A2 (fr) 1995-11-15
DE69132931D1 (de) 2002-03-21
GR3020505T3 (en) 1996-10-31
EP0558647B1 (fr) 1996-05-29
DK0995445T3 (da) 2009-07-06
ATE425764T1 (de) 2009-04-15
ATE213165T1 (de) 2002-02-15
US5210017A (en) 1993-05-11
AU657109B2 (en) 1995-03-02
WO1992008985A2 (fr) 1992-05-29

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