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CA2075493A1 - Amorces pcr pour la detection de l'espece legionella et methodes permettant de regler l'intensite visuelle dans les essais d'hybridation - Google Patents

Amorces pcr pour la detection de l'espece legionella et methodes permettant de regler l'intensite visuelle dans les essais d'hybridation

Info

Publication number
CA2075493A1
CA2075493A1 CA002075493A CA2075493A CA2075493A1 CA 2075493 A1 CA2075493 A1 CA 2075493A1 CA 002075493 A CA002075493 A CA 002075493A CA 2075493 A CA2075493 A CA 2075493A CA 2075493 A1 CA2075493 A1 CA 2075493A1
Authority
CA
Canada
Prior art keywords
nucleic acid
primers
primer
probes
capture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002075493A
Other languages
English (en)
Inventor
Teresa K. H. Picone
Theresa Mccallum
Michael A. Zoccoli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2075493A1 publication Critical patent/CA2075493A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/166Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002075493A 1990-12-20 1991-12-19 Amorces pcr pour la detection de l'espece legionella et methodes permettant de regler l'intensite visuelle dans les essais d'hybridation Abandoned CA2075493A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US63089990A 1990-12-20 1990-12-20
US630,899 1990-12-20
PCT/US1991/009688 WO1992011273A1 (fr) 1990-12-20 1991-12-19 Amorces d'amplification enzymatique du genome pour la detection des especes legionella et procedes de controle de l'intensite d'un signal visuel dans des dosages d'hybridation

Publications (1)

Publication Number Publication Date
CA2075493A1 true CA2075493A1 (fr) 1992-06-21

Family

ID=24529011

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002075493A Abandoned CA2075493A1 (fr) 1990-12-20 1991-12-19 Amorces pcr pour la detection de l'espece legionella et methodes permettant de regler l'intensite visuelle dans les essais d'hybridation

Country Status (5)

Country Link
EP (1) EP0533854A4 (fr)
JP (1) JPH05507206A (fr)
AU (1) AU657286B2 (fr)
CA (1) CA2075493A1 (fr)
WO (1) WO1992011273A1 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5470723A (en) * 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
US6174668B1 (en) * 1993-05-14 2001-01-16 Johnson & Johnson Clinical Diagnostics, Inc. Diagnostic compositions, elements, methods and test kits for amplification and detection of two or more target DNA's using primers having matched melting temperatures
EP0630973A3 (fr) * 1993-05-14 1995-04-26 Eastman Kodak Co Compositions de diagnostic, éléments, procédés et kits pour tests d'amplification et de détection de deux ou plusieurs ADN's utilisant des amorces à températures de fusion-adaptés.
US20020055101A1 (en) 1995-09-11 2002-05-09 Michel G. Bergeron Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
DE19515891C2 (de) * 1995-04-29 1999-10-28 Roche Diagnostics Gmbh Gattungs- und speziesspezifische Identifizierung von Legionellen
US20030049636A1 (en) 1999-05-03 2003-03-13 Bergeron Michel G. Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
US20100267012A1 (en) 1997-11-04 2010-10-21 Bergeron Michel G Highly conserved genes and their use to generate probes and primers for detection of microorganisms
US5968739A (en) * 1996-12-10 1999-10-19 Abbott Laboratories Nucleic acid primers and probes for detecting Legionella pneumophila
FR2775002B1 (fr) * 1998-02-19 2003-01-10 France Etat Procede de diagnostic d'agents pathogenes respiratoires par biologie moleculaire
EP2322668A3 (fr) 1999-09-28 2011-10-05 Geneohm Sciences Canada Inc. GENE A FORT POUVOIR DE CONSERVATION ET l'UTILISATION POUR PRODUIRE DES SONDES D'ACIDE NUCLEIQUE SPECIFIQUES A L'ESPECE, SPECIFIQUES AU GENE, SPECIFIQUES A LA FAMILLE, SPECIFIQUES AU GROUPE ET UNIVERSELLES ET DES SONDES D'AMPLIFICATION, EN VUE DE DETECTER ET D'IDENTIFIER RAPIDEMENT DES MICRO-ORGANISMES
FR2801677B1 (fr) * 1999-11-30 2003-04-25 Ulp Ct D Analyses Et De Rech S Procede d'analyse d'un echantillon pour la presence eventuelle de legionelles comprenant une etape d'immunocapture
US7368246B2 (en) 2005-09-15 2008-05-06 Primera Biosystems, Inc. Quantitative gene expression profiling
EP1939304A1 (fr) * 2006-12-29 2008-07-02 Nederlandse Organisatie voor Toegepast-Natuuurwetenschappelijk Onderzoek TNO Procédé de détection de souches de légionellose pathogéniques
EP2385990A4 (fr) * 2008-12-18 2012-08-01 Us Gov Health & Human Serv Détection de réaction en chaine par polymérase en temps réel de legionella pneumophila et forme de différenciation d'autres espèces de legionella
FR2960552B1 (fr) * 2010-06-01 2015-08-07 Ct Europ D Expertise Et De Rech Sur Les Agents Microbiens Ceeram Procede de genotypage de legionella pneumophila
DE102018213027A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Reaktionsgemisch, Verfahren und Kit zur Durchführung einer quantitativen Echtzeit-PCR
CN115029452B (zh) * 2021-11-16 2024-03-22 江汉大学 一种军团菌属的mnp标记位点、引物组合物、试剂盒及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
AU616646B2 (en) * 1986-11-24 1991-11-07 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US5004682A (en) * 1987-11-02 1991-04-02 Olin Corporation Method and kit for detecting live microorganisms in chlorine- or bromine-treated water
US4902624A (en) * 1987-11-23 1990-02-20 Eastman Kodak Company Temperature cycling cuvette
CA2033718A1 (fr) * 1990-01-19 1991-07-20 Ronald M. Atlas Procede de detection d'agents pathogenes microbiens dans l'eau et indicateurs de contamination par des matieres fecales humaines dans des echantillons d'eau et trousses de detection connexes

Also Published As

Publication number Publication date
EP0533854A4 (en) 1994-06-08
WO1992011273A1 (fr) 1992-07-09
EP0533854A1 (fr) 1993-03-31
JPH05507206A (ja) 1993-10-21
AU9156791A (en) 1992-07-22
AU657286B2 (en) 1995-03-09

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