CA2059114C - Methode et appareil de chromatographie liquide a haut rendement - Google Patents
Methode et appareil de chromatographie liquide a haut rendementInfo
- Publication number
- CA2059114C CA2059114C CA 2059114 CA2059114A CA2059114C CA 2059114 C CA2059114 C CA 2059114C CA 2059114 CA2059114 CA 2059114 CA 2059114 A CA2059114 A CA 2059114A CA 2059114 C CA2059114 C CA 2059114C
- Authority
- CA
- Canada
- Prior art keywords
- segment
- upstream
- sample
- segments
- downstream
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 42
- 238000000926 separation method Methods 0.000 title claims description 14
- 238000002086 displacement chromatography Methods 0.000 title description 2
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 46
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 41
- 150000001875 compounds Chemical class 0.000 claims abstract description 37
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 36
- 230000005526 G1 to G0 transition Effects 0.000 claims abstract description 35
- 239000000463 material Substances 0.000 claims abstract description 31
- 238000010828 elution Methods 0.000 claims abstract description 25
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 24
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 60
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 15
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- 239000012156 elution solvent Substances 0.000 claims description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 7
- 239000003480 eluent Substances 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 125000001183 hydrocarbyl group Chemical group 0.000 claims description 4
- 239000000377 silicon dioxide Substances 0.000 claims description 3
- 239000003960 organic solvent Substances 0.000 claims description 2
- 239000012530 fluid Substances 0.000 abstract description 8
- 238000009738 saturating Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 80
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 18
- 238000011068 loading method Methods 0.000 description 15
- 239000000203 mixture Substances 0.000 description 15
- 239000002904 solvent Substances 0.000 description 13
- 230000000694 effects Effects 0.000 description 7
- 239000007787 solid Substances 0.000 description 7
- 238000012856 packing Methods 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 230000000717 retained effect Effects 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 3
- 239000000356 contaminant Substances 0.000 description 3
- 238000011067 equilibration Methods 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 238000005086 pumping Methods 0.000 description 3
- 238000005194 fractionation Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000003607 modifier Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 108010091867 peptide P Proteins 0.000 description 2
- -1 peptides Chemical class 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000011877 solvent mixture Substances 0.000 description 2
- 101001006370 Actinobacillus suis Hemolysin Proteins 0.000 description 1
- 101150007148 THI5 gene Proteins 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000009699 differential effect Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002953 preparative HPLC Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2059114 CA2059114C (fr) | 1992-01-09 | 1992-01-09 | Methode et appareil de chromatographie liquide a haut rendement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2059114 CA2059114C (fr) | 1992-01-09 | 1992-01-09 | Methode et appareil de chromatographie liquide a haut rendement |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2059114A1 CA2059114A1 (fr) | 1993-07-10 |
| CA2059114C true CA2059114C (fr) | 1998-08-25 |
Family
ID=4149065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2059114 Expired - Fee Related CA2059114C (fr) | 1992-01-09 | 1992-01-09 | Methode et appareil de chromatographie liquide a haut rendement |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2059114C (fr) |
-
1992
- 1992-01-09 CA CA 2059114 patent/CA2059114C/fr not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2059114A1 (fr) | 1993-07-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |