CA2051071A1 - Production d'ammoniac - Google Patents
Production d'ammoniacInfo
- Publication number
- CA2051071A1 CA2051071A1 CA 2051071 CA2051071A CA2051071A1 CA 2051071 A1 CA2051071 A1 CA 2051071A1 CA 2051071 CA2051071 CA 2051071 CA 2051071 A CA2051071 A CA 2051071A CA 2051071 A1 CA2051071 A1 CA 2051071A1
- Authority
- CA
- Canada
- Prior art keywords
- gene
- nifl
- mutant
- bacteria
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 title claims abstract description 15
- 229910021529 ammonia Inorganic materials 0.000 title claims abstract 5
- 238000004519 manufacturing process Methods 0.000 title claims abstract 5
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 74
- 230000001105 regulatory effect Effects 0.000 claims abstract description 4
- 101150004139 nifL gene Proteins 0.000 claims abstract 12
- 101150035327 nifA gene Proteins 0.000 claims abstract 6
- 101150108916 nifA1 gene Proteins 0.000 claims abstract 6
- 241000589152 Azotobacter chroococcum Species 0.000 claims abstract 5
- 108010020943 Nitrogenase Proteins 0.000 claims abstract 3
- 230000000694 effects Effects 0.000 claims abstract 3
- 230000004048 modification Effects 0.000 claims abstract 2
- 238000012986 modification Methods 0.000 claims abstract 2
- 230000035772 mutation Effects 0.000 claims abstract 2
- 241000196324 Embryophyta Species 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 23
- 241000894006 Bacteria Species 0.000 claims description 21
- 230000012010 growth Effects 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 241000589151 Azotobacter Species 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 239000000443 aerosol Substances 0.000 claims description 2
- 241000589180 Rhizobium Species 0.000 claims 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims 1
- 241000589149 Azotobacter vinelandii Species 0.000 claims 1
- 108090001090 Lectins Proteins 0.000 claims 1
- 102000004856 Lectins Human genes 0.000 claims 1
- 241000219793 Trifolium Species 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 239000002523 lectin Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 239000002689 soil Substances 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 239000012634 fragment Substances 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 10
- 108020004414 DNA Proteins 0.000 description 8
- 238000011144 upstream manufacturing Methods 0.000 description 7
- ODPOAESBSUKMHD-UHFFFAOYSA-L 6,7-dihydrodipyrido[1,2-b:1',2'-e]pyrazine-5,8-diium;dibromide Chemical compound [Br-].[Br-].C1=CC=[N+]2CC[N+]3=CC=CC=C3C2=C1 ODPOAESBSUKMHD-UHFFFAOYSA-L 0.000 description 6
- 239000005630 Diquat Substances 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 2
- 239000005977 Ethylene Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- HSFWRNGVRCDJHI-UHFFFAOYSA-N alpha-acetylene Natural products C#C HSFWRNGVRCDJHI-UHFFFAOYSA-N 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 125000002534 ethynyl group Chemical group [H]C#C* 0.000 description 2
- 241000192542 Anabaena Species 0.000 description 1
- 102100027674 CTD small phosphatase-like protein Human genes 0.000 description 1
- 241000511343 Chondrostoma nasus Species 0.000 description 1
- 241000518994 Conta Species 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 101100285518 Drosophila melanogaster how gene Proteins 0.000 description 1
- 101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 1
- 101100007969 Homo sapiens CTDSPL gene Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 101150062031 L gene Proteins 0.000 description 1
- 101100270435 Mus musculus Arhgef12 gene Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 101150065098 glnG gene Proteins 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- -1 n1trogen Chemical compound 0.000 description 1
- 101150012415 ntrC gene Proteins 0.000 description 1
- 125000001820 oxy group Chemical group [*:1]O[*:2] 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 101150011750 rpoN gene Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2051071 CA2051071A1 (fr) | 1991-09-10 | 1991-09-10 | Production d'ammoniac |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2051071 CA2051071A1 (fr) | 1991-09-10 | 1991-09-10 | Production d'ammoniac |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2051071A1 true CA2051071A1 (fr) | 1993-03-11 |
Family
ID=4148339
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2051071 Abandoned CA2051071A1 (fr) | 1991-09-10 | 1991-09-10 | Production d'ammoniac |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2051071A1 (fr) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10384983B2 (en) | 2015-07-13 | 2019-08-20 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
| US10662432B2 (en) | 2011-06-16 | 2020-05-26 | The Regents Of The University Of California | Synthetic gene clusters |
| US11479516B2 (en) | 2015-10-05 | 2022-10-25 | Massachusetts Institute Of Technology | Nitrogen fixation using refactored NIF clusters |
| US11565979B2 (en) | 2017-01-12 | 2023-01-31 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
| US11678668B2 (en) | 2018-06-27 | 2023-06-20 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
| US11946162B2 (en) | 2012-11-01 | 2024-04-02 | Massachusetts Institute Of Technology | Directed evolution of synthetic gene cluster |
| US11993778B2 (en) | 2017-10-25 | 2024-05-28 | Pivot Bio, Inc. | Methods and compositions for improving engineered microbes that fix nitrogen |
| US12151988B2 (en) | 2017-10-25 | 2024-11-26 | Pivot Bio, Inc. | Gene targets for nitrogen fixation targeting for improving plant traits |
| US12281980B2 (en) | 2020-05-01 | 2025-04-22 | Pivot Bio, Inc. | Measurement of nitrogen fixation and incorporation |
| US12281299B2 (en) | 2019-03-19 | 2025-04-22 | Massachusetts Institute Of Technology | Control of nitrogen fixation in rhizobia that associate with cereals |
| US12391624B2 (en) | 2018-07-11 | 2025-08-19 | Pivot Bio, Inc. | Temporally and spatially targeted dynamic nitrogen delivery by remodeled microbes |
| US12421519B2 (en) | 2019-01-07 | 2025-09-23 | Pivot Bio, Inc. | Plant colonization assays using natural microbial barcodes |
| US12478068B2 (en) | 2020-05-01 | 2025-11-25 | Pivot Bio, Inc. | Stable liquid formulations for nitrogen-fixing microorganisms |
| US12497342B2 (en) | 2023-06-30 | 2025-12-16 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
-
1991
- 1991-09-10 CA CA 2051071 patent/CA2051071A1/fr not_active Abandoned
Cited By (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10662432B2 (en) | 2011-06-16 | 2020-05-26 | The Regents Of The University Of California | Synthetic gene clusters |
| US12209245B2 (en) | 2011-06-16 | 2025-01-28 | The Regents Of The University Of California | Synthetic gene clusters |
| US11946162B2 (en) | 2012-11-01 | 2024-04-02 | Massachusetts Institute Of Technology | Directed evolution of synthetic gene cluster |
| US10556839B2 (en) | 2015-07-13 | 2020-02-11 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
| US10919814B2 (en) | 2015-07-13 | 2021-02-16 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
| US10934226B2 (en) | 2015-07-13 | 2021-03-02 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
| US10384983B2 (en) | 2015-07-13 | 2019-08-20 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
| US11739032B2 (en) | 2015-07-13 | 2023-08-29 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
| US11479516B2 (en) | 2015-10-05 | 2022-10-25 | Massachusetts Institute Of Technology | Nitrogen fixation using refactored NIF clusters |
| US11565979B2 (en) | 2017-01-12 | 2023-01-31 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
| US11993778B2 (en) | 2017-10-25 | 2024-05-28 | Pivot Bio, Inc. | Methods and compositions for improving engineered microbes that fix nitrogen |
| US12151988B2 (en) | 2017-10-25 | 2024-11-26 | Pivot Bio, Inc. | Gene targets for nitrogen fixation targeting for improving plant traits |
| US11678667B2 (en) | 2018-06-27 | 2023-06-20 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
| US11678668B2 (en) | 2018-06-27 | 2023-06-20 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
| US12268212B2 (en) | 2018-06-27 | 2025-04-08 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
| US12471599B2 (en) | 2018-06-27 | 2025-11-18 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
| US11963530B2 (en) | 2018-06-27 | 2024-04-23 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
| US12290074B2 (en) | 2018-06-27 | 2025-05-06 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
| US12391624B2 (en) | 2018-07-11 | 2025-08-19 | Pivot Bio, Inc. | Temporally and spatially targeted dynamic nitrogen delivery by remodeled microbes |
| US12421519B2 (en) | 2019-01-07 | 2025-09-23 | Pivot Bio, Inc. | Plant colonization assays using natural microbial barcodes |
| US12281299B2 (en) | 2019-03-19 | 2025-04-22 | Massachusetts Institute Of Technology | Control of nitrogen fixation in rhizobia that associate with cereals |
| US12281980B2 (en) | 2020-05-01 | 2025-04-22 | Pivot Bio, Inc. | Measurement of nitrogen fixation and incorporation |
| US12478068B2 (en) | 2020-05-01 | 2025-11-25 | Pivot Bio, Inc. | Stable liquid formulations for nitrogen-fixing microorganisms |
| US12497342B2 (en) | 2023-06-30 | 2025-12-16 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
Similar Documents
| Publication | Publication Date | Title |
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| CA2051071A1 (fr) | Production d'ammoniac | |
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| Stewart et al. | N2-fixing cyanobacteria and their potential applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |