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CA2045614A1 - Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe - Google Patents

Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe

Info

Publication number
CA2045614A1
CA2045614A1 CA2045614A CA2045614A CA2045614A1 CA 2045614 A1 CA2045614 A1 CA 2045614A1 CA 2045614 A CA2045614 A CA 2045614A CA 2045614 A CA2045614 A CA 2045614A CA 2045614 A1 CA2045614 A1 CA 2045614A1
Authority
CA
Canada
Prior art keywords
cell sample
proliferation
cell
images
substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2045614A
Other languages
English (en)
Other versions
CA2045614C (fr
Inventor
James W. Bacus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cell Analysis Systems Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/315,289 external-priority patent/US5086476A/en
Application filed by Individual filed Critical Individual
Publication of CA2045614A1 publication Critical patent/CA2045614A1/fr
Application granted granted Critical
Publication of CA2045614C publication Critical patent/CA2045614C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1765—Method using an image detector and processing of image signal
    • G01N2021/177—Detector of the video camera type

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Sustainable Development (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Méthode et appareil de traitement des images (10) donnant un indice de prolifération d'un échantillon de cellules par coloration des cellules à l'aide d'un chromogène comme substance indicative de la prolifération et par contre-coloration pour le dénombrement des noyaux des cellules. Le chromogène est activé par un conjugué anticorps-enzyme qui se lie à la substance de prolifération pour produire un échantillon de cellules colorées. L'échantillon de cellules colorées est observé à l'aide d'un microscope optique (12), faisant partie intégrante de l'appareil, qui donne une image agrandie de l'échantillon de cellules. L'appareil filtre optiquement (18) l'image de l'échantillon et produit une paire d'images optiquement améliorées de la substance de prolifération et du noyau des cellules. Les images améliorées sont analysées électroniquement pour déterminer le nombre de noyaux de cellules et la quantité de substance de prolifération apparaissant dans les images respectivement. Les résultats sont alors comparés pour créer un indice de prolifération pour la portion de l'échantillon de cellules apparaissant dans l'image de l'échantillon.
CA002045614A 1989-02-24 1990-02-23 Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe Expired - Fee Related CA2045614C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US315,289 1989-02-24
US07/315,289 US5086476A (en) 1985-11-04 1989-02-24 Method and apparatus for determining a proliferation index of a cell sample

Publications (2)

Publication Number Publication Date
CA2045614A1 true CA2045614A1 (fr) 1990-08-25
CA2045614C CA2045614C (fr) 1997-09-30

Family

ID=23223738

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002045614A Expired - Fee Related CA2045614C (fr) 1989-02-24 1990-02-23 Appareil servant a determiner l'indice de proliferation d'un echantillon de cellules et methode connexe

Country Status (3)

Country Link
EP (1) EP0460071A4 (fr)
CA (1) CA2045614C (fr)
WO (1) WO1990010277A1 (fr)

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US6026174A (en) * 1992-10-14 2000-02-15 Accumed International, Inc. System and method for automatically detecting malignant cells and cells having malignancy-associated changes
US5889881A (en) * 1992-10-14 1999-03-30 Oncometrics Imaging Corp. Method and apparatus for automatically detecting malignancy-associated changes
BRPI0507159A (pt) 2004-02-02 2007-06-26 Ambrx Inc polipeptìdeos em feixe de quatro hélices humanos modificados e seus usos
WO2006069220A2 (fr) 2004-12-22 2006-06-29 Ambrx, Inc. Hormone de croissance humaine modifiee
KR20070034512A (ko) 2004-06-18 2007-03-28 암브룩스, 인코포레이티드 신규 항원-결합 폴리펩티드 및 이의 용도
MX2007000728A (es) 2004-07-21 2007-03-15 Ambrx Inc Polipeptidos biosinteticos que utilizan amino acidos no naturalmente codificados.
US8080391B2 (en) 2004-12-22 2011-12-20 Ambrx, Inc. Process of producing non-naturally encoded amino acid containing high conjugated to a water soluble polymer
CA2590429C (fr) 2004-12-22 2014-10-07 Ambrx, Inc. Compositions de synthetase d'aminoacyl-arnt et utilisations correspondantes
CA2594561C (fr) 2004-12-22 2014-12-23 Ambrx, Inc. Formulations d'hormone de croissance humaine comportant un acide amine code de maniere non naturelle
GB2438982A (en) 2004-12-22 2007-12-12 Ambrx Inc Compostions containing, methods involving, and uses of non-natural amino acids and polypeptides
WO2006133088A2 (fr) 2005-06-03 2006-12-14 Ambrx, Inc. Molecules d'interferons humains ameliorees et utilisations de celles-ci
JP4829969B2 (ja) 2005-08-18 2011-12-07 アンブルックス，インコーポレイテッド ｔＲＮＡ組成物、およびその使用
JP2009520949A (ja) 2005-11-16 2009-05-28 アンブルックス，インコーポレイテッド 非天然アミノ酸を含んでいる組成物および方法
WO2008030558A2 (fr) 2006-09-08 2008-03-13 Ambrx, Inc. Polypeptide plasmatique humain modifié ou squelettes de fc et leurs utilisations
PT2061878E (pt) 2006-09-08 2014-04-22 Ambrx Inc Supressor híbrido arnt para células de vertebrados
ES2465473T3 (es) 2006-09-08 2014-06-05 Ambrx, Inc. Transcripción de arnt supresor en células de vertebrado
JP2010523084A (ja) 2007-03-30 2010-07-15 アンブルックス，インコーポレイテッド 修飾ｆｇｆ−２１ポリペプチド
WO2008137471A2 (fr) 2007-05-02 2008-11-13 Ambrx, Inc. Polypeptides d'interféron bêta modifiés et leurs utilisations
KR101656107B1 (ko) 2007-11-20 2016-09-08 암브룩스, 인코포레이티드 변형된 인슐린 폴리펩티드 및 이의 용도
CA2712606A1 (fr) 2008-02-08 2009-08-13 Ambrx, Inc. Polypeptides leptine modifies et leurs utilisations de marburg
NZ591235A (en) 2008-07-23 2012-07-27 Ambrx Inc Modified bovine g-csf polypeptides comprising non natural amino acid and their uses treating infections such as mastitis
BRPI0919403A2 (pt) 2008-09-26 2017-09-26 Ambrx Inc polipeptídeos modificados de eritropoetina animal e seus usos
DK2337846T3 (en) 2008-09-26 2018-03-12 Ambrx Inc NON-NATURAL AMINO ACID REPLICATION DEPENDENT MICROORGANISMS AND VACCINES
PE20121707A1 (es) 2009-12-21 2012-12-17 Ambrx Inc Polipeptidos de somatotropina bovina modificados y sus usos
BR112012015597A2 (pt) 2009-12-21 2017-01-31 Ambrx Inc peptídeos de somatotropina suínos modificados e seus usos
KR101963460B1 (ko) 2010-08-17 2019-03-28 암브룩스, 인코포레이티드 변형된 릴랙신 폴리펩타이드 및 그것의 용도
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
AR083006A1 (es) 2010-09-23 2013-01-23 Lilly Co Eli Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas
JP6355082B2 (ja) 2013-07-18 2018-07-11 パナソニックＩｐマネジメント株式会社 病理診断支援装置及び病理診断支援方法
CN107108710B (zh) 2014-10-24 2022-02-15 百时美施贵宝公司 修饰的fgf-21多肽及其用途
MX2019008449A (es) 2017-02-08 2019-09-09 Bristol Myers Squibb Co Polipetidos de relaxina modificada que comprenden un mejorador farmacocinetico y sus usos.
CN111413504B (zh) * 2020-04-03 2022-01-28 河北医科大学第四医院 一种辅助判读ki67增殖指数的标准比对卡

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4125828A (en) * 1972-08-04 1978-11-14 Med-El Inc. Method and apparatus for automated classification and analysis of cells
US3999047A (en) * 1972-09-05 1976-12-21 Green James E Method and apparatus utilizing color algebra for analyzing scene regions
US4199748A (en) * 1976-11-01 1980-04-22 Rush-Presbyterian-St. Luke's Medical Center Automated method and apparatus for classification of cells with application to the diagnosis of anemia
JPS5661650A (en) * 1979-10-24 1981-05-27 Omron Tateisi Electronics Co Analyzing device of cell
US4741043B1 (en) * 1985-11-04 1994-08-09 Cell Analysis Systems Inc Method of and apparatus for image analyses of biological specimens

Also Published As

Publication number Publication date
CA2045614C (fr) 1997-09-30
EP0460071A4 (en) 1993-02-03
WO1990010277A1 (fr) 1990-09-07
EP0460071A1 (fr) 1991-12-11

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