BRPI0900320A2 - product and process for revitalization of natural soil biota and plant growth - Google Patents

Abstract

THE PRODUCT AND PROCESS FOR REVITALIZING NATURAL SOIL BIOTA AND VEGETABLE GROWTH consists of primary and secondary macronutrients and micronutrients already known by the state of the art, suspending agents, emulsifying agents, protective agents for accelerated growth of microorganisms, allantoin, glycolic extract. seaweed, gelatin, sorbitol, glycerol, amino acids, natural gums, bentonite clay, charcoal and activated carbon, dehydrated milk, polyvinyl pyrrolidone (PVP-K), glucose, crustacean ground shells and hydrolyzate containing mixtures of collagen, blood and meat, sequestering or complexing agents, organic substrates, natural growth stimulant, cross-linking agent, gradual release compound for coating macronutrients and living microorganisms with nematicidal function. The PRODUCTION PROCESS is carried out through the composition preparation and the mixing procedure, being Phase A the preparation of growth culture, adaptation and selection of living organisms; Phase B of organic substrate preparation; Micronutrient preparation phase C; Phase D of the growth additive and Phase E of the preparation of controlled release macronutrients. The manufacturing procedure consists of mixing the Phase B components to the Phase E components, then adding the prepared nutrients by mixing the C and D phases. Then making the B, E, C and D mixture and allowing to compost for a long period. After optimum composting has occurred, inoculate this mixture with the phase A components, allowing to ferment controlled for a selected fermentation to occur. Finally, to have products in the form of powder or bran, the classification is made by vibrating sieves. For products that need to be marketed in granular form, the granulation process occurs after composting mixtures B, E, C and D in a traditional market granulator and dryer equipment. The product resulting from the mixing of the phase A components is inoculated into the ready granules, where they will be fermented for a predetermined period of time, after which the fertilizer is sent for commercialization.

Description

PRODUCT AND PROCESS FOR BIOTANATURAL REVITALIZATION OF SOILS AND PLANT GROWTH

APPLICATION FIELD

The present invention, pertaining to the fertilizer sector, relates to a product consisting of primary and secondary macronutrients, micronutrients, "living" microorganisms (selected bacteria and fungi) and selected organic matter, called agricultural biochemical complex, developed for soil application to crops. annual and perennial, developed to replace traditional fertilizers and provide greater agronomic efficiency when applied to degraded and phytononematoid-infested soils and their production process.

GOAL

The product is designed to perform several functions:

1. Restore, prevent and control the cover of degraded soil caused by successive and intensive agricultural activities and phytomatode infestations;

2. Fertilize macro and micronutrients with slow release, providing better utilization of nutrients, less environmental damage to the water table by leaching effect and reduction of losses due to evaporation;

3. Promote or naturally stimulate vegetative growth with natural substances, similar to natural hormones;

4. Being a product of specific application in the soil will be used, to be formulated after chemical analysis of the soil and prior identification of the phytomatode species present.5. It can be applied with any kind of equipment available on the market, that is, it can be produced in powder, bran and granular form.

SUMMARY OF THE INVENTION

"NATURAL BIOTA REVITALIZATION PRODUCT DESOLOS AND VEGETABLE GROWTH" is made up of primary and secondary micronutrient macronutrients, known from the state of the art, suspending agents (xanthan gum, modified clay and cellulose esters or mixtures thereof) emulsifying agents (esters). sorbitan, ethoxylated desorbitan esters and gelatin or mixtures thereof, protective agents for accelerated growth of microorganisms (starches and modified starches, sugarcane bagel, rice bran, polyethylene glycol, aloe vera (aloe vera), allantoin, glycolic extract). seaweed, gelatin, sorbitol, glycerol, amino acids, natural gums, bentonite clay, charcoal and activated carbon, dihydrate, polyvinyl pyrrolidone (PVP-K), glucose, hydrolyzed crustacean shells containing mixtures of collagen, blood and meat or mixtures thereof ), sequestering or complexing agents (citric acid, ascorbic acid, EDTA1 HEDTA and Iignosulfonates or mixtures thereof), organic substrates (biofertilizers, seedling production substrates, soil conditioners, filter cake, crop residues, bark and remnants of wood, sugarcane bagasse, animal manure, cellulose , peat, composted fruit peels, cereal peels, activated charcoal, composted sewage sludge and starches or mixtures thereof, natural growth stimulant (metalloid iodine, potassium hydroxide, stevia oustevioside, zinc acetate, sodium citrate, amino acids containing tryptophan, tryptophan, isopropyl alcohol, ascorbic acid, anhydrous citric acid, lactic acid, oxalic acid, salicylic acid, formic acid, butyric acid, propionic acid, sucrose, mannitol, sorbitol, water soluble B-complex vitamins, B-complex vitamins, vitamin E, Vitamin E acetate or mixtures thereof), cross-linking agent (formaldehyde and glutaraldehyde or mixture thereof) (ra), Gradual release compound for the coating of macronutrients (sodium and potassium stearate, sodium sticate, rosin, polyterpenes of vegetable origin, vegetable oils and animal tallow or mixtures thereof) and living microorganisms with nematicidal function (bacteria, viruses, fungi or mixtures thereof).

The "PRODUCTION PROCESS" is performed through the stages of preparation of the composition and the mixing procedure, being the Stage A preparation of growth culture, adaptation and selection of living organisms, Phase B of preparation of the organic substrate; Phase C of micronutrient preparation; Phase D of the growth additive and Phase E of the preparation of controlled release macronutrients. The manufacturing procedure consists of mixing the Phase B components to the Phase E components, then adding the nutrients prepared by mixing the CeD phases. Then mix B1E1C and D and let compost for a long time. After optimal conjugation has occurred, inoculate this mixture with the components of phase A, allowing to ferment controlled to occur a selected fermentation.

Finally, to have products in the form of powder or bran, the classification is made through vibrating sieves. For products that need to be marketed in granular form, the granulation process takes place after composting mixtures B, E, C and D in a traditional granulator and dryer equipment. The product resulting from the mixing of the phase A components is inoculated into the ready granules, where they will be fermented for a predetermined period of time, after which the fertilizer is sent to the market.

TECHNICAL STATE

"Society's concern about the impact of agriculture on the environment and the contamination of the food chain with pesticides has been changing the agricultural scene, resulting in the presence of market segments eager for differentiated products, both those produced without the use of pesticides, such as those bearing stamps. These pressures have led to the development of more sustainable crop systems and therefore less dependent on pesticide use. The concept of sustainable agriculture involves the proper management of natural resources, avoiding environmental degradation to meet the needs This approach alters the priorities of conventional farming systems in relation to the use of non-renewable sources, especially energy, and shifts the view on the appropriate balance between food production and environmental impacts.

The changes imply a reduction in the dependence on chemicals and other energy inputs and the greater use of biological processes in agricultural systems.

Plant protection in conventional methods through the use of pesticides has extremely attractive features such as simplicity, predictability and the need for little understanding of the basic agroecosystem processes for its application. For example, to be successful with the application of a broad spectrum herbicide, knowledge of how to apply the product is important and little information is required on the ecology and physiology of species. Many biological control studies take a similar approach, where the encounter between pathogen antagonist or predator prey is emphasized. Such a strategy is appropriate for relatively aggressive and specific predators, but is of less value in more complex situations. In such cases, after the introduction of, for example, a microbially controlled microbial agent, it will be established in a niche, followed by interaction with the target organism and other species of organisms. These complex interactions are critical to successful control and should be holistically analyzed and considered in the long term rather than in the short term. "

"In contrast to conventional agriculture, alternative systems seek to take advantage of naturally occurring interactions. Alternative systems emphasize the management of biological relationships, such as those between pests and predators, and natural processes such as nitrogen fixation rather than chemical methods. The goal is to increase the sustainability of the biological interactions on which agricultural production is based, rather than reducing and simplifying these interactions.

One of the main problems of sustainable agriculture concerns the control of diseases, pests and invasive plants. Several techniques used to minimize the damage caused by these phytosanitary problems contaminate the environment or cause changes that compromise the sustainability of the system. "

The intensive use of pesticides in agriculture has been recognized to have promoted several environmental problems, such as contamination of food, soil, water and animals; the intoxication of farmers; resistance of pathogens, pests and weeds to certain pesticides; the biological balance, altering the cycling of nutrients and organic matter, the elimination of beneficial organisms; and the reduction of biodiversity.

Much of the pesticides applied in the field are lost. It is estimated that about 90% of the pesticides applied do not reach the target, being dissipated to the environment and having as a final point water reservoirs and, mainly, the soil. Losses are generally due to improper application, both in terms of technology and timing. In some cases, because the application was made to protect against a pest or pathogen that is not present in the area. This is because calendars-based sprays are still performed and not when the problem occurs. The use of a significant amount of chemicals would be avoided if control measures were taken only when economic damage was achieved. "

The continued and exclusive use of pesticides has resulted in the occurrence of pests or pathogens resistant to certain products, which are always diagnosed. Thus, these pesticides continue to be applied, even though their efficiency is compromised by resistance in the target organism. The effects of these applications on non-target organisms can also cause serious agro-ecosystem imbalances. The emergence of iatrogenic diseases (those that occur due to pesticide use) is an example of problems that can occur. "

"Plant diseases occur in nature with the objective, in particular, to maintain the biological balance and nutrient cycling, and, from the point of view, are beneficial. What is observed is that diseases and pests occur in endemic form. They do not occur. epidemics that could destroy plant species, as it would endanger the survival of pathogens and pests, but epidemics are frequent in agricultural ecosystems Human interference, altering the balance of nature, results in the occurrence of epidemics. Increased population of pathogens and pests epidemically is the cultivation of genetically homogeneous plants, which is contrary to the diversity of varieties. "

"Techniques such as biological and physical control are also being developed and many are being used such as: thermotherapy of fruits and seeds; solar energy for soil phytopathogen control (solarization); ultraviolet radiation for postharvest pathogen control ; the use in curtain greenhouses that filter out certain wavelengths with consequent disease and pest control; citrus plants against the sadness of citrus and pumpkin plants against common mosaic; Baculovirus anticarsia for the control of soybean; of the wood wasp with the nematode Delademussiricidicola, the Bacillus thuringiensis for the control of larvae of lepidoptera, the control of the sugarcane borer with Cotesia flavipes, the control of numerous pests with neem oil and extract (Azadirachta indica) ·, milk raw cow meat for the control of powdery mildew, the control of pathogens carried by the causative soil tumbling smoke with Trichoderma et al. Also integrated management techniques and ecological management of pests and diseases lead to significant reductions in pesticide use, with environmental and economic advantages. "

"Another important aspect is the nutritional balance of plants. Fertilization is usually based on the needs of NPK1 not considering micronutrients and other elements that may be beneficial to plants. Several studies show the effects of nutrients on plant diseases, and consequently the reduction of nutrient content. need for control with balanced plant nutrition. "

The use of organic matter, either by incorporation into the soil or after transformation for later use, should be considered as an alternative method of controlling diseases, pests and invasive plants. One of the known transformations is anaerobic or aerobic digestion, the product of which is called biofertilizer, and can be used for foliar sprays or direct soil applications. One of the main characteristics of biofertilizer is the presence of microorganisms responsible for the decomposition of organic matter, gas production and release of metabolites, among them antibiotics and hormones. Thus, the more active and diversified the biofertilizer raw material, the greater the possibility of release of different organic substances. In addition, biofertilizer acts due to the considerable effect on plants, due to the presence of macro and micronutrients. Thus, the joint action of several mechanisms is responsible for the control obtained.

The effect of organic matter sources on the severity of plant diseases depends on the type of material used, the C: N ratio and the time taken for incorporation. In general, suppressive soils show greater microbiota activity than conducive soils. Thus, the regular addition of adequate sources of organic matter can induce suppressivity by stimulating the activity of primary decomposers, mainly bacteria, fungi and other organisms such as mites, nematodes and arthropods, such as Collembola, which may play an important role in the control of phytopathogens. Primary decomposers may act as antagonists of phytopathogens by pornutrient competition, antibiosis and parasitism, while micro and mesofaunas may contribute to predation control. A classic example of organ system disease control is the suppression of Phytophthora cinnamomi emabacate in Australia. High levels of organic matter were kept in the superficial layers of the orchards soils. The main mechanism of action was the increase of hyphae lysis by bacteria and actinomycetes. The predatory activity damicrofauna, in particular of several amoebas, was also increased in soils with high organic matter content. The leading soils contained amoebasimilar but less densely populated. "(BETTIOL, Wagner; GHINI, Raquel" Plant Protection in Alternative Agricultural Systems "in" Plant Protection in Sustainable Agriculture ", ISBN 85-87459-06-6, Sami J Michereff and Reginaldo Barros Editores, Federal Rural University of Pernambuco, Recife, PE, 2001).

"Biological control is defined by Cook & Baker as the reduction in the sum of inoculum or disease-determining activities caused by a pathogen by or through one or more non-human organisms. In disease-determining activities, growth is involved. infectivity, virulence, and other qualities of the pathogen, or processes that determine infection, the development of symptoms, and production.In organisms, individuals or virulent or hypovirulent populations are included within the pathogenic species, the genetically engineered host or cultural practices, or microorganisms for greater or more effective resistance against the pathogen, and pathogen antagonists, defined as the microorganisms that interfere with survival or disease-determining activities caused by lymphopathogens.Thus, according to these authors, biological control can be accompanied by: cultural practices to create an environment favorable to antagonists and host plant resistance or both; plant improvement to increase resistance to the pathogen or to adapt the host to antagonist activities; mass introduction of non-pathogenic antagonists and strains or other beneficial organisms or agents.

The definition of Cook & Baker is the most accepted by the scientific community. However, mass introduction of antagonists is the most practiced and studied form of biological control. Thus, many define biological control as the control of one microorganism through another microorganism. Commercially this is the definition used, and the following commercial products and their respective agents are available on the market: AQ10 (Ampelomycesquisqualis), Aspire (Candida oleophila), Binab T (Trichoderma harzianum and T.polysporum), Biofox C (Fusarium oxysporum - non-pathogenic), Bio-Fungus (Trichoderma), Bio-Save 100, Bio-Save 110 and Bio-Save 1000 (Pseudomonassyringae), BIightBan A506 (Pseudomonas fluorescens) , Blue Circle (Burkholderiacepacea), Conquer (Pseudomonas fluorescens), Contans (Coniothyriumminitans), Deny (Burkholderia cepacea), Epic (Bacillus subtilis) , Kodiak, Kodiak HB and Kodiak AT (Bacillus subtilis), Koni (Coniothyrium minitans), Mycostop (Streptomyces griseovirides), Nogall, Diegall (, Agrobacterium radiobacter), Norbac 84C (Agrobacterium radiobac) ter), Phagus (bacteriophages), Polygandron (Pythium oligandrum), Promote (Trichodermaharzianum and T. viride), PSSOL (Pseudomonas solanacearum - non-pathogenic), Rhizo-Plus and Rhizo-Plus Konz. (Bacillus subtilis), RootShieId T-22G (Trichodermaharzianum), Rotstop, e.g. (Phlebia gigantea), Serenade (Bacillus subtilis), SoiIGard (Gliocladium virens), Supresivit (Trichoderma harzianum), System 3 (Bacillus subtilis GB03), T-22G, T-22 Planter Box (Trichoderma harzianum), Triehodex (Trichoderma harzianum) , Trichopel, Trichoject, Trichodowels eTrichoseal (Trichoderma harzianum), Trichoderma 2000 (Trichoderma sp.) And Victus (Pseudomonas fluorescens) ". (BETTIOL, Wagner;" Alternative Methods for Plant Disease Control "in" Sustainable Plant Protection, " ISBN 85-87459-06-6, Sami J. Michereff and Reginaldo BarrosEditors, Federal Rural University of Pernambuco, Recife, PE 2001).

"Parasitic plant nematodes and arbuscular mycorrhizal fungi-AMF may be simultaneously associated with the roots, so the combined effect of both groups of organisms on plant development should be considered."

"Most of these interactions involve endoparasitemental nematodes, probably due to their worldwide importance as phytopathogens and because they are easily propagated. Several authors have confirmed that the mycorrhizal association leads to the reduction of damage caused by phytopathogenic nematodes in several crops of economic interest, including cotton. , cowpea, citrus, beans, tomatoes and soybeans However, in experiments with grapevines, increased damage caused by nematodes in mycorrhizal plants has been reported.

The effects of mycorrhization on the reproduction of pathogen nematodes in various host plants are shown in Table 1.

Table 1 - Effects of mycorrhization on reproduction pathogenic denematodes

<table> table see original document page 11 </column> </row> <table> <table> table see original document page 12 </column> </row> <table>

Fungi also play an important role in the biocontrol of insects, especially those with a sucking mouthpiece, such as Hyphomycetes, such as the genera Metarrhizium spp., Beauveriaspp., Nomurea riIey spp., Verticillum Iecanii, and Cladosporium spp.

PI0405316-8 reports predatory fungal (Arthrotrysspp., Duddingtonia spp. And Monacrosporium spp.) Formulations of nematodes for use in the control of verminosis in domestic animals without harming the agroecosystems in order to use them in the control of domestic gastrointestinal nematodesioses. .

In the agricultural area, various inoculant technologies have been developed, such as US 4,337,077 (agricultural inoculant composition including bacteria, peat, and calcium phosphate), BR PI8604810-4 (usable legaculture inoculant, using dried and disinfected peat or sterilized gamma irradiation). and mixed with Rhizobium sp.), BR PI8601804 (agricultural inoculant of lyophilized bacteria (Rhizobium sp or others) mixed with a chemically inert porous granular carrier), BR PI9103905 (Rhizobium spp. inoculant lyophilized with a cell protector in place of peat support); BR PI9903634-7 (organo-mineral compound containing various natural selections of fermented bacteria, fungi and algae and inoculated in phosphate rock and organic material for these microorganisms to solubilize the rock), BR PI9400025-5 (granular nutrient containing carboxylate magnesium and crystallized saccharide, indicated as soil pest controller such as nematodes and insects), BR Pl9500340-1 (formulation for plant parasitic nematode control, having Pasteuria spp, natural enemy of the nematode, suspended in water); MXPA04004414 (product consisting of amino acid and culture of the fungus Myrotheciumverrucaria or equivalent); CN 101165016 (preparation process comprising the following steps: 1- preparation of obiofertilizer materials; 2- high temperature fermentation and decay; 3- Paecilomyces Iilacinus addition and culture; 4- secondary fermentation; 5- palletizing; and 7- packing); CN 101125774 (biofertilizer composed of a mixture of evericin with ammonium sulfate, monoammonium phosphate, lemery, borax, zinc sulfate, green copperas, calcium powder, root agent, brown sugar, chicken, ground pepper, peanut butter or cake ); CN 1958529 (the product comprises the components Ν, P205 and K20, and secondary additives and trace elements and traditional Chinese medicines; traditional Chinese medicines contain a mixture of Stemona japonica, Glycyrrhiza uralensis and Wisteria sinensis for nematode cyst and fungal control in soybeans. ); US 2004242424 (Fertilizer includes at least phosphoric acid and phosphorous acid and the salts, salts of phosphorus, potassium, calcium, sodium, magnesium, manganese, zinc, copper and iron and a microorganism substance extracted from fungi, bacteria or viruses); and JP58023611 (a porous material - vermiculite or straw) is mixed with non-pathogenic germ to give a fertilizer, which is used as the soil bed, and nematode predators, for example, Dactylella ellipsospora, belonging to the Hyphomyeetes Fungos subclass Imperfeeti (Deuteromycetes) are mixed with the fertilizer in an amount of about 103-109 germs / g dry material).

The product object of this patent, which can be considered under the concept of fertilizer "organic mineral fertilizer with biological revitalizer" or "biofertilizer" is not yet in the list of products of the Ministry of Agriculture, Livestock and Supply - MAPA. The product was tested in trials in pots, greenhouse and agricultural areas of various regions of Brazil, and the agronomic results obtained are presented in the examples above.

Among the advantages obtained with the product object of this patent, in addition to the chemical and organic fertilization of the soil, with the consequent nutrient recoveries and the C / N ratio, the farmer can also treat phytomatode contaminated areas in a single operation, reducing application costs, replacing conventional pesticides with "environmentally friendly" biological control products, "restoring soil biotanatics" while fertilizing the soil.

DESCRIPTION OF THE FIGURES

Figure 1 shows the flowchart of the product production process in powder or bran form.

Figure 2 shows the flowchart of the grain production process.

DETAILED DESCRIPTION

PRODUCTION PROCESS

The mixing process described relates to product production and the amounts should be proportionally adjusted according to the interest of the farmer.

Phase A - Adaptation and Growth of Microorganisms

1- In an industrial blender or tank, preferably stainless steel, with stirrer a mixture (standard dispersion) is mixed in 10 to 20 L water of: modified starch (5 to 10 kg), rice bran (1 to 10 kg) ), sugarcane bagasse (10 to 20 kg), hydrolysed from collagen, beef and bovine blood mixtures (1 to 2 kg), PVP-K90 (2 to 5 g), allantoin (10 to 50 g), ground crustacean shells (1 to 5 kg), polyvinyl alcohol (2 to 10 g), glucose (10 to 100 g), Soy Iecithin (5 to 50 g), gelatin (10 to 150 g), sorbitan ester (20 to 100 g) , xanthan gum (2 to 50 g), ammonium phosphate (10 to 200 g), ground bone meal (1 to 2 kg) and phase B mixture substrates (5 to 10 kg);

2- Stir the mixture between 500 and 1000 rpm for 1 to 3 minutes;

3- Add selected matrices of hyphae or mycelium fungi and increase the rotation between 1500 to 2500 rpm for 5 minutes;

4- Separate the mixture into 1 to 2 kg plastic bags;

5- Leave in oven for approximately 7 days with controlled temperature between 20 ° to 30 ° C and in the absence of light.

Phase B - Organic Substrate Production

1- In a heated mixing equipment or box of 1,000 to 20,000L1 containing bottom-mounted high-speed dispersing stirrer, add in the following order: potassium hydroxide (1 to 5 kg) and / or calcium hydroxide (virgin lime) ( 5 to 10 kg), hydrolyzed from mixtures of collagen, beef and blood (2 to 5 L) and clean water (5 to 100 L), maintaining the temperature between 70 ° to 90 ° C and stirring between 200 to 300rpm;

2- Add charcoal (5 to 10 kg), fruit peels (5 to 10 kg), peat (50 to 100 kg) and composted organic materials (100 to 150 kg), stirring between 200 and 500 rpm for 2 hours;

3- Add industrial phosphoric acid with 50 to 55% P205 (1 to 5 kg) and stir at 100 to 500 rpm for 1 h;

4- Remove the mixture from the tank and dry, preferably in filter press, until humidity is between 10 and 20% by weight.

Phase C - Preparation of Micronutrients 1. In a small container of approximately 10 liters, with or with mechanical scrubber, add 5 to 7 liters of cold water, citric acid (10 to 20 g) and sodium molybdate (20 to 50 g) and proceed to mechanical agitation or manually until complete solubilization;

2- In a 20 L container or tank with or without mechanical stirrer, add 7 to 10 L of cold water, citric acid (5 to 10 g) or sodium EDTA (5 to 10 g) with iron sulphate Il (10 50 g) until completely dissolved, then add 200 ν hydrogen peroxide (20 to 50 ml) by gently stirring and add manganese sulfate (20 to 60 g), zinc sulfate (50 to 100 g), boric acid (20 to G) and shake the total dissolution;

3- Mix items 1 and 2 by shaking by hand or mechanically lowering the corresponding rotation until total phase dissolution.

D - Natural Stimulant

1- In a 5 L volumetric flask, provided with mechanical stirring, electric heater and reflux condenser, add 1 L of cold water;

2- Add isopropyl alcohol (50 to 100 mL) and metalloid iodine (1 to 2 g) and stir at 2000 at 3000 rpm for 10 minutes,

3- Add potassium hydroxide (20 to 30 g), stevioside (50 to 100 g) and boil for 2 hours by refluxing the condensate;

4- Add citric acid (10 to 20 g), oxalic acid (2 to 3 g), salicylic acid (2 to 3 g), lactic acid (0.5 to 1.0 g), formic acid (1 to 2 g ), amino acids (100 to 500 g) or tryptophan (0.1 to 0.2 g), zinc acetate (5 to 10 g) and sodium nitrate (50 to 80 g), allowing to stir between 500 and 1000 rpm for 2 hours at 60 to 80 ° C;

5- Add ethoxylated sorbitan esters 20 mo (10 to 20 g), butyric acid (1 to 5 g), propionic acid (2 to 5 g) and stir between 1000 to 2000rpm, lowering and maintaining the temperature between 20 to 25 ° C 6- Add sucrose (1 to 2 kg), mannitol (20 to 35 g), sorbitol (1 to 50 g), B-complex vitamins (0.5 to 10 g), ascorbic acid (1 to 50 g) evitamin E acetate (5 to 10 g);

7. Correct the pH with citric acid and / or ammonia or triethanolaminane in the range of 6.00 to 7.00 and keep the mixture under stirring at 500 to 1000 rpm for 12 hours at room temperature.

Phase E - Macronutrients and Release Coating CompoundGradual

1 - In a 50 L tank with stirring and heating, add 5 or 10 L hydrolyzate containing mix of collagen, blood and meat, formaldehyde (1 to 5 L), sodium and potassium stearate (5 kg), disodium silicate (1 5 kg), pitch (1 to 2 kg), polyterpenes of plant origin (0.5 to 1 kg), vegetable oils (0.5 to 1 L) and beef tallow (1 to 5 kg).

2- Heat between 70 and 80 ° C, with stirring between 500 and 1000 rpm, stirring for 2 hours and then cool to room temperature.

3- Transfer to spray equipment and apply thin mist solution under a mixture of secondary primary macronutrients in a dryer, preferably fluidized bed.

Order of mixtures for the manufacture of the Product, as can be seen in Figures 1 and 2, for ease of understanding:

1- Mix Phase B with Phase E evenly in a mixer equipment;

2- Mix Phase C with Phase D evenly in a tank mixer;

3- Mix items 1 (Β + E) and 2 (C + D) above and allow to compost for a period of approximately 7 to 20 days; 4- After this period, inoculate with phase A product (5 to 10%). mix) and mix evenly by letting it ferment for 5 to 50 days under strict temperature (20 to 50 ° C) and relative humidity (25 to 50%) control and away from sunlight;

5- For products in the form of powder or bran, the classification is preferably made in granulometric sieves;

6- For granulated products, the granulation process takes place after the composting of the (B + E) + (C + D) mixture which passes through the sieve and goes directly to a granulator equipment;

7- After granulation, the mixture should be inoculated with the phase A product (5 to 10% by mass), then going to a solar shelter with relative humidity control (15 to 20%);

8- Product packaging for marketing PRODUCT

Table 2 presents the generic composition of the product.

Table 2: Generic product composition (% by mass)

<table> table see original document page 18 </column> </row> <table>

There is no preferred value for product components.

Primary and secondary macronutrients and micronutrients are those normally used by the art, provided that they are compatible with the product, in a dosage to be defined according to the culture and the need for the soil for that culture.

Organic material, expressed as a percentage of mass, is composed of organic substrate, natural growth stimulant, macronutrient coating, living microorganisms.

"NATURAL BIOTA REVITALIZATION PRODUCT DESOLOS AND VEGETABLE GROWTH" is made up of primary and secondary micronutrient macronutrients, known from the state of the art, suspending agents (xanthan gum, modified clay and cellulose esters or mixtures thereof) emulsifying agents (esters). sorbitan, ethoxylated desorbitan esters and gelatin or mixtures thereof, protective agents for accelerated growth of microorganisms (starches and modified starches, sugarcane bagel, rice bran, polyethylene glycol, aloe vera (aloe vera), allantoin, glycolic extract). seaweed, gelatin, sorbitol, glycerol, amino acids, natural gums, bentonite clay, charcoal and activated carbon, dihydrate, polyvinyl pyrrolidone (PVP-K), glucose, hydrolyzed crustacean shells containing mixtures of collagen, blood and meat or mixtures thereof ), sequestering or complexing agents (citric acid, ascorbic acid, EDTA, HEDTA and Iignosulfonates or mixtures thereof), organic substrates (biofertilizers, substrates for seedling production, soil conditioners, filter cake, composted bark and scraps, castor cake, sugarcane bagasse, animal manure, cellulose, peat, composite fruit peels, cereal husks, charcoal or activated carbon, composted sludge and starches or mixtures thereof, natural growth stimulant (iodomethoid, potassium hydroxide, stevia or stevioside, zinc acetate, sodium citrate, tryptophan-containing amino acids, tryptophan , isopropyl alcohol, ascorbic acid, anhydrous citric acid, lactic acid, oxalic acid, salicylic acid, formic acid, butyric acid, propionic acid, sucrose, mannitol, sorbitol, water-soluble B-complex vitamins, Vitamin E, Vitamin E acetate or B complex vitamins mixtures thereof), cross-linking agent (formaldehyde and glutaraldehyde or their mixture), gradual use for the coating of macronutrients (sodium and potassium stearate, sodium silicate, rosin, polyterpenes of vegetable origin, vegetable oils, gelatin, animal-based polyhydroxyalkanoates and mixtures thereof) and living microorganisms with nematicide function (bacteria, viruses, fungi or their mixtures). As the product is a "live microorganism fertilizer", after mixing is completed during fermentation, the likelihood of fungal growth, ie mass increase, will be inevitable. Therefore, at the time of product marketing, chemical analysis and supplementation should be made to adjust the macro and micronutrient warranties.

The application of the product may be in powder or bran or granular form or mixtures thereof and may be carried out manually or mechanized. Table 3 presents suggestions for generic fertilization for planting several crops with conventional fertilizer, in the dosage 03-12-06, where it is recommended to prepare soil-specific biofertiizer after the analysis of nutrients and contamination by phytomatodes.

Table 3 - Recommendations for no-till and conventional tillage:

<table> table see original document page 20 </column> </row> <table> <table> table see original document page 21 </column> </row> <table>

EXAMPLE

Production of 1000 Kg of NPK 03-12-06 biofertilizer containing micronutrients and 10% of living microorganisms.

Table 4 - Macronutrient Mass Balance

<table> table see original document page 21 </column> </row> <table>

Table 5 - Micronutrient Mass Balance

<table> table see original document page 21 </column> </row> <table> <table> table see original document page 22 </column> </row> <table>

Table 6 - Organic material and microorganisms

<table> table see original document page 22 </column> </row> <table>

Claims (20)

1. "PRODUCT FOR REVITALIZATION OF NATURAL SOIL BIOTA VEGETABLE GROWTH" consisting of secondary and micronutrient primary macronutrients, characterized in that it contains suspending agents, emulsifying agents, protective agents for accelerated growth of microorganisms, sequestering or complexing agents, natural growth stimulants , crosslinking agent, compound of gradual release for the coating of macronutrients and live microorganisms with nematicidal function. 2. "PRODUCT FOR REVITALIZING NATURAL VEGETABLE SOIL BIOTA" according to claim 1, characterized in that the suspending agents are xanthan gum, gellan, clay-modified gum and cellulose esters or mixtures thereof. "PRODUCT FOR REVITALIZING NATURAL VEGETABLE SOIL BIOTA" according to claim 1, characterized in that the emulsifying agents are sorbitan esters, sorbitanethoxylated esters, gelatin or mixtures thereof. 4. "PRODUCT FOR REVITALIZATION OF NATURAL VEGETABLE SOIL NATURAL BIOTA" according to claim 1, characterized in that the protective agents for accelerated growth of microorganisms are starch and modified starch, rice bran, soybean iecithin, polyethylene glycol, aloe vera (aloe vera), allantoin, seaweed extratoglycolic, gelatin, sorbitol, glycerol, amino acids, natural gums, bentonite clay, charcoal and activated charcoal, dehydrated milk, polyvinyl pyrrolidone (PVP-K), glucose, and shellfish containing hydrolyzed mixtures of collagen, blood and meat or mixtures thereof. 5. "PRODUCT FOR REVITALIZING NATURAL BIOTA FROM SOIL AND VEGETABLE GROWTH" according to claim 1, characterized in that the sequestering or complexing agents are citric acid, ascorbic acid, etidronic acid, EDTA, HEDTA and lignosulfonates or mixtures thereof. 6. "PRODUCT FOR REVITALIZATION OF NATURAL SOIL BIOTA AND VEGETABLE GROWTH" according to claim 1, characterized in that the organic substrates are biofertilizers, substrates for seedling production, soil conditioners, filter cake, crop residues, cascase composted woods, castor cake, sugarcane bagasse, animal manure, cellulose, peat, composted fruit peels, decereal peels, charcoal or activated charcoal, composted sewage sludge and starches or their mixtures. 7. "PRODUCT FOR REVITALIZING NATURAL SOIL BIOTA VEGETABLE GROWTH" according to claim 1, characterized in that the natural growth stimulants are metalloid iodine, potassium hydroxide, stevia or stevioside, zinc acetate, sodium citrate, amino acids containing tryptophan, tryptophan, isopropyl alcohol, ascorbic acid, anhydrous citric acid, lactic acid, oxalic acid, salicylic acid, formic acid, butyric acid, propionic acid, sucrose, mannitol, sorbitol, water soluble B vitamins, B complex vitamins, vitamin 8. A "PRODUCT FOR REVITALIZING NATURAL VEGETABLE SOIL BIOTA" according to claim 1, characterized in that the cross-linking agents are formaldehyde, glutaraldehyde or a mixture thereof. 9. "PRODUCT FOR REVITALIZATION OF NATURAL VEGETABLE SOIL BIOTA" according to claim 1, characterized in that the gradual release compounds for coating of macronutrients are sodium and potassium stearate, rosin, polyterpenes of plant origin, vegetable oils, gelatin, polyhydroxyalkanoates and tallow of animal origin or mixtures thereof). 10. "PRODUCT FOR REVITALIZING NATURAL BIOTA OF VEGETABLE SOIL AND SOIL" according to claim 1, characterized in that the living micro-organisms with nematicidal function are bacteria, viruses, fungi or mixtures thereof. 11. "PRODUCTION PROCESS" characterized in that it is carried out through the stages of preparation of the composition and the mixing procedure, being Stage A of preparation of growth culture, adaptation and selection of living organisms; Phase B of organic substrate preparation; Phase C micronutrient preparation; Phase D of the Growth Additive and Edo Phase Preparation of the controlled release macronutrients, and the manufacturing procedure consists of mixing the Phase B components with the Phase E components, then adding the prepared nutrients to the CeDe phases mixture and then mixing the resulting products. and allow composting for a period of time until optimum compost is injected into this mixture with the Phase A components, allowing fermentation to be controlled to a selected fermentation and finally grading the product for marketing. 12. "PRODUCTION PROCESS" according to Claim 11, characterized in that Phase A - Adaptation and Growth of Microorganisms is a mixture of modified starch (5 to 10 kg), rice bran (1 to 10 kg), bagacinho. sugarcane (10 to 20 kg), hydrolysed from collagen, beef and blood mixtures (1 to 2 kg), PVP-K90 (2 to 5 g), allantoin (10 to 50 g), ground crustacean shells (1 to 5 Kg), polyvinyl alcohol (2 to 10 g), glucose (10 to 100 g), Soy Iecithin (5 to 50 g), gelatin (10 to 150 g), sorbitan ester (20 to 100 g), xanthan gum ( 2 to 50g), ammonium phosphate (10 to 200g), ground bone meal (1 to 2kg) and phase B mixture substrates (5 to 10kg); stirring the mixture at 500 to 1000 rpm for 1 to 3 minutes; add the selected hyphae or mycelium arrays of nematophagous fungi, bacteria and viruses and increase the rotation between 1500 to 2500 rpm for 5 minutes; Separate the mixture into portions of 1 to 2 kg and leave in oven for approximately 7 days with controlled temperature between 20 ° to 30 ° C and in the absence of light. 13. "PRODUCTION PROCESS" according to Claim 11, characterized in that Phase B - Organic Substrate Production consists of mixing potassium hydroxide (1 to 5 kg) or calcium hydroxide (5 to 10 kg). mixtures of collagen, beef and blood (2 to 5 L) and clean water (5 to 100 L), maintaining the temperature between 70 ° to 90 ° C and agitation between 200 to 300 rpm; then add charcoal (5 to 10 kg), fruit peels (5 to 10 kg), peat (50 to 100 kg) and composite organic materials (100 to 150 kg), stirring 200 to 500 rpm for 2 hours; industrial phosphoric acid with 50 to 55% P205 (1 to 5 kg) and stir 100 to 500 rpm for 1 h; and remove the mixture from the tank and wipe until moisture is 10 to 20% by weight. 14. "PRODUCTION PROCESS" according to Claim 11, characterized in that Step C - Preparation of Micronutrients consists of preparing item 1 by mixing cold water (5 to 7 L), citric acid (10 to 20 g. ) and sodium molybdate (20 to 50 g) and mechanical or manual stirring until complete solubilization; prepare item 2 by mixing cold water (7 to 10 L), citric acid (5 to 10 g) or sodium EDTA (5 to 10 g) with iron sulphate Il (10 to 50 g) until completely dissolved, and then add 200 ν hydrogen peroxide (20 to 50 mL) and manganese sulfate (20 to 60 g), zinc sulfate (50 to 100 g), boric acid (20 to 50 g) while stirring until completely dissolved, and without mixing items 1 and 2 by shaking manually or mechanically at the corresponding low rotation until the phase is completely dissolved. 15. "Production process" according to Claim 11, characterized in that Step D - Natural Stimulant consists of adding alcoholic propyl alcohol (50 to 100 mL) and metalloid iodine (1 to 2 g) in cold water (1 L) and stirring. 2000 at 3000 rpm for 10 minutes; then add potassium hydroxide (20 to 30 g), stevioside (50 to 100 g) and boil for 2 hours refluxing the condensate; add citric acid (10 to 20 g), oxalic acid (2 to 3 g), salicylic acid (2 to 3 g), lactic acid (0.5 to 1.0 g), formic acid (1 to 2 g), amino acids ( 100 to 500 g) or tryptophan (0.1 to 0.2 g), zinc acetate (5 to 10 g) and sodium citrate (50 to 80 g), allowing to stir between 500 and 1000 rpm for 2 hours at room temperature. 60 to 80 ° C, add 20 mo ethoxylated sorbitan esters (10 to 20 g), butyric acid (1 to 5 g), propionic acid (2 to 5 g) and stir at 1000 to 2000 rpm, lowering keeping the temperature between 20 to 25 ° C; add sucrose (1 to 2 kg), mannitol (20 to 35 g), sorbitol (1 to 50 g), B-complex vitamins (0.5 to 10 g), ascorbic acid (1 to 50 g) and vitamin E acetate (5 to 10 g); make the pH correction with citric acid and / or ammonia or triethanolamine in the range of 6.00 to -7.00 and keep the mixture under stirring at 500 to 1000 rpm for 12 hours at room temperature. 16. "Production process" according to Claim 11, characterized in that Step E - Macronutrients and Gradual Release Coating Compound consists of mixing formaldehyde (1 to 5L), sodium and potassium stearate (5 kg), silicate. sodium (1 to 5 kg), pitch (1 to 2 kg), polyterpenes of plant origin (0.5 to 1 kg), vegetable oils (0.5 to 1 L) and beef tallow (1 to 5 kg) in hydrolyzate containing mix of collagen, blood and meat (5 or 10 L); heat at 70 to 80 ° C under stirring at 500 to 1000 rpm and keep under stirring for 2 hours and cool to ambient temperature; then transfer to a sprayer and apply fine mist solution over a mixture of primary and secondary macronutrients in a dryer. 17. "Production process" according to Claim 11, characterized in that Stage E - Macronutrients and Gradual Release Coat Compound consists of spraying fine mist gelatin or polyhydroxyalkanoates onto a mixture of primary and secondary macronutrients in a dryer. 18. "Production process" according to Claim 11, characterized in that the mixing order for the manufacture of the powder or bran product is: -1- Mixing Phase B with Phase E evenly; - Mix Phase C with Phase D - 3 - Mix items 1 (Β + E) and 2 (C + D) above and leave in Ieiras for approximately 7 to 20 days - 4- Inoculate with product Phase A (5 to 10% by mass) mix evenly and allow to ferment for 5 to 50 days under strict temperature control (20 to 50 ° C) and relative humidity (25 to 50%) and protected from sunlight; classification for products in powder or crumbly form; - 6- Pack for marketing 19 "PRODUCTION PROCESS" according to claim 11, characterized in that the mixing order for the manufacture of the formagranulated product is: - 1 - Mixing Phase B with Phase E evenly; Phase D; - 3- Mix items 1 (Β + E) and 2 (C + D) above and leave to compose in Ieiras for approximately 7 to 20 days; - 4- Proceed to classification, referral to granulator equipment and - 5- Inoculate with phase A product (5 to 10% by mass) and mix uniformly - 6- Ferment for 5 to 50 days under strict control of temperature (20 to 50 ° C) and relative humidity (25 to 50 %) and to the shelter of sunlight; - 7- Pack for commercialization.