[go: up one dir, main page]

BRPI0819495A2 - "fragmento de ácido nucleico isolado, construção recombinante, célula de planta e método para reduzir a expressão de uma sequência alvo" - Google Patents

"fragmento de ácido nucleico isolado, construção recombinante, célula de planta e método para reduzir a expressão de uma sequência alvo"

Info

Publication number
BRPI0819495A2
BRPI0819495A2 BRPI0819495-5A2A BRPI0819495A BRPI0819495A2 BR PI0819495 A2 BRPI0819495 A2 BR PI0819495A2 BR PI0819495 A BRPI0819495 A BR PI0819495A BR PI0819495 A2 BRPI0819495 A2 BR PI0819495A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
target sequence
plant cell
acid fragment
reducing expression
Prior art date
Application number
BRPI0819495-5A2A
Other languages
English (en)
Inventor
Brian Mcgonigle
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BRPI0819495A2 publication Critical patent/BRPI0819495A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
BRPI0819495-5A2A 2007-12-18 2008-12-17 "fragmento de ácido nucleico isolado, construção recombinante, célula de planta e método para reduzir a expressão de uma sequência alvo" BRPI0819495A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1451007P 2007-12-18 2007-12-18
PCT/US2008/087136 WO2009079548A2 (en) 2007-12-18 2008-12-17 Down-regulation of gene expression using artificial micrornas

Publications (1)

Publication Number Publication Date
BRPI0819495A2 true BRPI0819495A2 (pt) 2015-03-10

Family

ID=40428299

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0819495-5A2A BRPI0819495A2 (pt) 2007-12-18 2008-12-17 "fragmento de ácido nucleico isolado, construção recombinante, célula de planta e método para reduzir a expressão de uma sequência alvo"

Country Status (9)

Country Link
US (5) US8115055B2 (pt)
EP (4) EP2222858B1 (pt)
CN (2) CN101903525B (pt)
BR (1) BRPI0819495A2 (pt)
CA (5) CA2895044C (pt)
RU (2) RU2492239C2 (pt)
UA (1) UA101823C2 (pt)
WO (1) WO2009079548A2 (pt)
ZA (1) ZA201004123B (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9062317B2 (en) 2011-05-09 2015-06-23 E I Du Pont De Nemours And Company Methods and compositions for silencing gene families using artificial microRNAs
IN2014DN02042A (pt) 2011-10-28 2015-05-15 Du Pont
AU2013212260A1 (en) 2012-01-23 2014-08-28 H. Damude Modifying the fatty acid profile of Camelina sativa oil
ES2635115T3 (es) 2012-01-23 2017-10-02 E. I. Du Pont De Nemours And Company Regulación por disminución de expresión génica utilizando micro ARN artificiales para silenciar genes biosintéticos de ácidos grasos
EP2836240B1 (en) 2012-04-10 2019-03-13 Georgia State University Research Foundation, Inc. Compositions and methods for treating otitis media and other conditions with inhibitors of cyld
CN105473722A (zh) 2013-03-11 2016-04-06 先锋国际良种公司 用于改善植物中化学信号扩散的方法及组合物
US20160024518A1 (en) 2013-03-13 2016-01-28 Brian McGonigle Production of small interfering rnas in planta
WO2014153254A2 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International Inc. Compositions and methods to control insect pests
EP3137607B1 (en) 2014-04-30 2020-12-02 Fondazione Edmund Mach Plant srna extract or plant mirna for use as immunosuppressive agent
US20170247719A1 (en) 2014-09-17 2017-08-31 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
MX2017016119A (es) 2015-06-16 2018-03-07 Pioneer Hi Bred Int Composiciones y metodos para controlar plagas de insectos.
WO2017218207A1 (en) 2016-06-16 2017-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CA3029666A1 (en) * 2016-06-30 2018-01-04 Cold Spring Harbor Laboratory Control of meiotic crossover in maize
US20210292778A1 (en) 2016-07-12 2021-09-23 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
WO2018148001A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International Inc Insecticidal combinations of plant derived insecticidal proteins and methods for their use
US20200165626A1 (en) 2017-10-13 2020-05-28 Pioneer Hi-Bred International, Inc. Virus-induced gene silencing technology for insect control in maize
EP4314277A1 (en) 2021-03-26 2024-02-07 Flagship Pioneering Innovations VII, LLC Compositions and methods for producing circular polyribonucleotides
WO2022204466A1 (en) 2021-03-26 2022-09-29 Flagship Pioneering Innovations Vii, Llc Production of circular polyribonucleotides in a prokaryotic system
WO2022204464A1 (en) 2021-03-26 2022-09-29 Flagship Pioneering Innovations Vii, Llc Production of circular polyribonucleotides in a eukaryotic system

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
WO1990011361A1 (en) 1989-03-17 1990-10-04 E.I. Du Pont De Nemours And Company External regulation of gene expression
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
DE69133128T2 (de) 1990-04-12 2003-06-18 Syngenta Participations Ag, Basel Gewebe-spezifische Promotoren
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
US5604121A (en) 1991-08-27 1997-02-18 Agricultural Genetics Company Limited Proteins with insecticidal properties against homopteran insects and their use in plant protection
ZA927576B (en) 1991-10-04 1993-04-16 Univ North Carolina State Pathogen-resistant transgenic plants.
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5428148A (en) 1992-04-24 1995-06-27 Beckman Instruments, Inc. N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
WO1994002620A2 (en) 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5470353A (en) 1993-10-20 1995-11-28 Hollister Incorporated Post-operative thermal blanket
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5750868A (en) 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
AU3495297A (en) 1996-07-08 1998-02-02 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
DE69838613T2 (de) 1997-07-22 2008-08-07 Pioneer Hi-Bred International, Inc. Gene, welche den phytat-metabolismus kontrollieren und daraus entstehende anwendungen
WO1999043797A2 (en) 1998-02-26 1999-09-02 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
EP1056862A1 (en) 1998-02-26 2000-12-06 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
EP1104469B1 (en) 1998-08-20 2005-11-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
AU5788299A (en) 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from (end) genes
RU2164944C1 (ru) * 1999-12-09 2001-04-10 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ изменения генетических свойств организма
US6512165B1 (en) 2000-07-10 2003-01-28 Pioneer Hi-Bred International, Inc. Methods for enhancing plant transformation frequencies
WO2002059324A2 (en) 2001-01-12 2002-08-01 Pioneer Hi-Bred International, Inc. Novel inositol polyphosphate kinase genes and uses thereof
WO2003027243A2 (en) 2001-09-27 2003-04-03 Pioneer Hi-Bred International, Inc. Phytate polynucleotides and methods of use
US20040268441A1 (en) 2002-07-19 2004-12-30 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
US20050144669A1 (en) 2003-07-01 2005-06-30 Whitehead Institute For Biomedical Research MicroRNAs in plants
WO2005047505A2 (en) 2003-08-07 2005-05-26 Whitehead Institute For Biomedical Research Methods and products for expression of micro rnas
WO2005035769A2 (en) * 2003-10-09 2005-04-21 E. I. Du Pont De Nemours And Company Gene silencing by using micro-rna molecules
BRPI0516874A (pt) * 2004-10-12 2008-09-23 Univ Rockefeller micrornas
US20060200878A1 (en) * 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
AU2006203892B2 (en) 2005-01-07 2011-03-24 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University Method to trigger RNA interference
EP1874935B2 (en) 2005-04-04 2023-10-18 E. I. du Pont de Nemours and Company Polynucleotides and methods for making plants resistant to fungal pathogens
US20100163019A1 (en) 2008-10-10 2010-07-01 Michel Chornet Conversion of cellulosic biomass to sugar

Also Published As

Publication number Publication date
CA2895049A1 (en) 2009-06-25
US8415526B2 (en) 2013-04-09
EP2573180B1 (en) 2015-03-25
US20120107939A1 (en) 2012-05-03
CN103898114B (zh) 2016-06-29
RU2492239C2 (ru) 2013-09-10
CA2894910C (en) 2016-10-11
EP2573180A2 (en) 2013-03-27
CA2709333C (en) 2016-06-21
CA2894910A1 (en) 2009-06-25
EP2573181A3 (en) 2013-06-26
WO2009079548A2 (en) 2009-06-25
EP2573182A2 (en) 2013-03-27
US20120115234A1 (en) 2012-05-10
CN101903525B (zh) 2014-04-23
CN101903525A (zh) 2010-12-01
CA2895048C (en) 2016-10-11
US8273951B2 (en) 2012-09-25
WO2009079548A3 (en) 2009-09-17
CA2895048A1 (en) 2009-06-25
US20110033937A1 (en) 2011-02-10
EP2222858B1 (en) 2013-02-13
ZA201004123B (en) 2011-08-31
EP2222858A2 (en) 2010-09-01
CA2895044C (en) 2016-07-05
EP2573180A3 (en) 2013-06-26
CA2895044A1 (en) 2009-06-25
US8536405B2 (en) 2013-09-17
EP2573181B1 (en) 2015-03-25
RU2013126413A (ru) 2014-12-20
CA2709333A1 (en) 2009-06-25
EP2573181A2 (en) 2013-03-27
US8115055B2 (en) 2012-02-14
UA101823C2 (uk) 2013-05-13
RU2010129428A (ru) 2012-01-27
US20120329159A1 (en) 2012-12-27
CN103898114A (zh) 2014-07-02
EP2573182A3 (en) 2013-06-26
US20090155909A1 (en) 2009-06-18
EP2573182B1 (en) 2015-03-25
CA2895049C (en) 2016-10-11

Similar Documents

Publication Publication Date Title
BRPI0819526A2 (pt) "fragmento de ácido nucléico isolado, construção recombinante, célula de planta e método para reduzir a expressão de uma sequência alvo"
BRPI0819495A2 (pt) "fragmento de ácido nucleico isolado, construção recombinante, célula de planta e método para reduzir a expressão de uma sequência alvo"
BRPI0820647A2 (pt) Polipeptídeo isolado, polinucleotídeo isolado, construção de ácido nucleico, célula hospedeira recombinante, métodos para produzir o polipeptídeo e um mutante de uma célula percursora, célula mutante, planta transgênica, parte de planta ou célula de planta, molécula de rna inibidora de filamento duplo, e, métodos para inibir a expressão de um polipeptídeo um uma célula e para produzir uma proteína e para degradar um xilano
BR112012002137A2 (pt) método para um polipeptídeo e/ou vírus de interesse em uma cultura de célula contínua,e, composição
BRPI0909611A2 (pt) ''polinucleotídeo isolado, construção de dna recombinante, célula, oleaginosa transgênica, método de aumento do teor total de ácido graxo de oleginosas, método de aumento do teor total de ácido graxo de uma célula de oleaginosa, fragmento de ácido nucleico, fragmento de ácido nucleico isolado e célula fúngica''
BRPI0814459A2 (pt) Célula de inseto, método para produzir um vírion parvoviral recombinante em uma célula de inserto, e, construção de ácido nucleico.
BRPI0820613A2 (pt) Polipeptídeo isolado, construção de ácido nucléico, célula hospedeira recombinante, métodos de produzir o polipeptídeo, de produzir um mutante de uma célula precursora, de inibir a expressão de um polipeptídeo tendo atividade de endoglicanase em uma célula, de degradar ou converter um material celulósico, de produzir um produto de fermentação e de produzir uma proteína, planta transgênica, parte de planta ou célula vegetal, molécula de rna inibidora de filamento duplo, construção de ácido nucléico, e, célula hospedeira recombinante
BRPI0817471A2 (pt) Polipeptídeo isolado, polinucleotídeo isolado, construção de ácido nucleico, célula hospedeira recombinante, métodos para produzir o polipeptídeo para produzir um mutante de uma célula precursora, para inibir a expressão de um polipeptídeo em uma célula, para produzir uma proteína, e para degradar um xilano acetilado, planta transgênica, parte de planta ou célula de planta, e , moléculas de rna inibidoras de filamento duplo
BRPI0819541A2 (pt) "enzima mutante cetoácido reductoisomerase, molécula de ácido nucléico, célula recombinante e métodos para evolução e a identificação de uma enzima cetoácido reductoisomerase e para a produção de isobutanol"
BRPI0820615A2 (pt) Construto de ácido nucleico, célula microbiana hospedeira recombinante, métodos para produzir um polipeptídeo tendo atividade de acetil-xilano esterase, para produzir um mutante de uma célula precursora, de inibir a expressão de um polipeptídeo, para produzir uma proteína e para degradar um material contendo xilano
BRPI0811364A2 (pt) "separador de aço inoxidável para uma célula de combustível e método para manufaturar o separador de aço inoxidável para célula de combustível"
BRPI0821088A2 (pt) construção de ácido nucléico, célula hospedeira microbiana transgênica, e, métodos para produzir um polipeptídeo tendo atividade de feruloil esterase, para produzir um mutante de uma célula-mãe, para inibir a expressão de um polipeptídeo tendo atividade de feruloil esterase em uma célula, para produzir uma proteína, e, para degradar um material contendo xilano.
DK2459715T3 (da) Fremgangsmåde til at oprense rekombinant adamts13 og andre proteiner og sammensætninger deraf
CL2012003373A1 (es) Huesped recombinante que comprende uno o mas genes de la biosintesis de esteviol; ácido nucleico aislado que comprende dichos genes; metodo para producir esteviol, un glucósido de esteviol o una composicion de glucósido de esteviol; cepa de saccharomyces recombinante; composicion de glucósido de esteviol.
BR112012031844A2 (pt) cepa bacteriana recombinante, processo para aperfeiçoar a utilização de xilose em uma cepa bacteriana recombinante e método para produção de etanol
BRPI1008634A2 (pt) "um meio de conexão, um método de fabricação do mesmo e uma conexão de material".
CL2013000338A1 (es) Proteina de union que tiene la capacidad de unirse a interleuquina 1beta y a la interleuquina 17; conjugado de proteina de union cristalizada; acido nucleico, vector, célula huesped; metodo para producir una proteina de unión; composicion farmacéutica que comprende la proteina de unión.
DK2152892T3 (da) Fremgangsmåde til indføring af nukleinsyrer i fungale celler
WO2010127304A3 (en) Sequencing methods
BRPI0906104A2 (pt) "derivado de ácido nuclêico peptídico, composição farmacêutica contendo uma quantidade "terapeuticamente eficaz do derivado de ácido nuclêico peptídico, composição farmacêutica contendo uma quantidade terapeuticamente eficaz do derivado de ácido nuclêico peptídico,método de utilização do derivado de ácido nuclêico peptídico e composto"
EP2470642A4 (en) CELL EXPANSION METHOD
BRPI1010623A2 (pt) composição e método para o controle de doenças vegetais
ITPC20070012A1 (it) Impianto e metodo per la vinificazione
BRPI0810418A2 (pt) Membros de ligação alvo, ácido nucléico isolado, vetor de expressão, célula hospedeira, composição, métodos para produzir um membro de ligação alvo, para o tratamento ou prevenção de asma e para produzir um anticorpo contra il-25
BR112012006670A2 (pt) célula bacteriana gram-negativa recombinante, e, método para produzir uma proteína recombinante

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Technical examination (opinion): publication of technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]