BRPI0619242A2 - protein, methods for increasing seed production and / or increasing plant growth rate and growth rate, plant cell construction, and methods for producing a transgenic plant to improve plant growth characteristics and growth characteristics relative to corresponding wild type plants, to increase crop yield, plant seed count and plant seed yield - Google Patents

Abstract

PROTEIN, METHODS TO INCREASE SEED PRODUCTION AND / OR INCREASE PLANT GROWTH RATE AND GROWTH RATE, PLANT CELL, CONSTRUCTION, AND METHODS TO PRODUCE A TRANSGENIC PLANT, TO IMPROVE CHARACTERISTIC CHARACTERISTICS OF CHARACTERISTICS. CORRESPONDENT WILD TYPE PLANTS, TO INCREASE VEGETABLE PRODUCTION, NUMBER OF SEEDS IN PLANTS AND SEED PRODUCTION IN PLANTS. The present invention generally relates to the field of molecular biology and relates to a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GRP (Growth-Related Protein). The present invention also relates to plants having modulated expression of a nucleic acid encoding a GRP, whose plants have improved growth characteristics compared to corresponding wild-type plants or other control plants. The invention also provides constructs useful in the methods of the invention. GRP can be one of the following: Seed Production Regulator (SYR), FG-GAP, CYP90B, CDC27, AT-hook transcription factors, DOF transcription factors and Cycline Dependent Kinase Inhibitors (CKIs).

Description

"PROTEIN, METHODS FOR INCREASING SEED PRODUCTION AND / OR INCREASING PLANT GROWTH RATE AND GROWTH RATE, VEGETABLE CELL, CONSTRUCTION, AND METHODS FOR PRODUCING A TRANSGENIC PLANT FEATURES OF CROSS GROWTH CHARACTERISTIC CHARACTERISTICS MATCHING WILD TYPE PLANTS TO INCREASE VEGETABLE PRODUCTION, NUMBER OF PLANT SEEDS AND PLANT SEED PRODUCTION "

The present invention generally relates to the field of molecular biology and relates to a method for enhancing various plant growth characteristics by modulating expression in a plant by providing nucleic acid encoding a GRP (Growth Related Protein).

The present invention also relates to plants having modulated expression of a GRP-encoding nucleic acid whose plants have improved growth characteristics over corresponding wild-type plants or other control plants. The invention also provides constructs useful in the methods of the invention.

Given the ever-growing world population, and the diminished land area available for agriculture, remains a major research objective to improve agricultural efficiency and improve plant diversity in horticulture. Conventional means for cultural and horticultural breeding use selective breeding techniques to identify plants having desirable characteristics. However, such selective breeding techniques have several disadvantages, in other words that these techniques are typically labor intensive and result in plants that often contain heterogeneous genetic complements that may not always result in the desirable trait being passed on from parent plants. Advances in molecular biology have allowed humans to manipulate the germplasm of animals and plants. Plant genetic engineering requires the isolation and manipulation of genetic material (typically in the form of DNA or RNA) and the subsequent introduction of such material; genetic in a plant. Such technology has led to the development of plants having various improved economic, agronomic or horticultural characteristics. Characteristics of particular economic interest are growth characteristics such as high production. Production is usually defined as the measurable economic value production of a crop. This can be defined in terms of quantity and / or quality. Yield is directly dependent on several factors, for example organ number and size, plant architecture (eg number of branches), seed yield and more. Root development, nutrient absorption and stress tolerance can also be important factors in determining yield.

Seed production is a particularly important feature, since the seeds of many plants are important for human and animal nutrition. Crops such as corn, rice, wheat, canola and soya account for more than half of total human caloric consumption, either through consumption of their own seeds or through consumption of meat products created from processed seeds. They are also a source of sugars, oils and many types of metabolites used in industrial processes. Seeds contain an embryo (the source of new shoots and roots) and an endosperm (the source of nutrients for embryo growth during germination and during early seedling growth). Seed development involves many genes, and requires the transfer of metabolites from the roots, leaves and stems to the developing seed. Endosperm, in particular, assimilates the metabolic precursors of carbohydrates, oils and proteins and synthesizes them into storage macromolecules to fill the grain.

Another important feature for many crops is early vigor. Improving early vigor is an important objective of modern rice breeding programs in both temperate and tropical rice cultivars. Long roots are important for proper ground anchorage in water-sown rice. Where rice is sown directly in flooded fields, and where plants must emerge rapidly through water, larger shoots are associated with vigor. Where furrow sowing is practiced, larger mesocotyls and coleoptils are important for good seedling emergence. Early vigor can also result from increased plant fitness because, for example, plants are better adapted to their environment (i.e. being better able to cope with various abiotic or biotic stressors). Plants having early vigor also show better crop establishment (with the crop growing more evenly, i.e. with most plants reaching various stages of development at substantially the same time), and show better growth and often better yield.

An additional important feature is that of improved abiotic stress tolerance. Abiotic stress is a primary cause of crop loss around the world, reducing average yields for most major cultivars by more than 50% (Wang et al, Planta (2003) 218: 1-14). Abiotic stresses can be caused by drought, salinity, temperature extremes, chemical toxicity and oxidative stress. The ability to improve plant tolerance to abiotic stress would be of great economic advantage to farmers around the world and would allow crop cultivation during adverse conditions and in territories where crop cultivation may not otherwise be possible.

Crop yield can therefore be increased by optimizing one of the factors mentioned above.

Depending on use, modification of certain production characteristics may be favored over others. For example for applications such as forage or wood production, or biofuel resource, an increase in the leafy parts of a plant may be desirable, and for applications such as wheat, starch or oil production, an increase in seed parameters may be desirable. particularly desirable. Even among seed parameters, some may be favored over others, depending on the application. Various mechanisms can contribute to increased seed yield, either in the form of increased seed size or increased seed number.

One approach to increasing (seed) production in plants may be by modifying the inherent growth mechanisms of a plant. One such mechanism is the cell cycle.

It has now been found that various growth traits can be improved in plants by modulating expression in a plant of a nucleic acid encoding a GRP (Growth Related Protein) in a plant. GRP can be one of the following: Seed Production Regulator (SYR), FG-GAP, CYP90B, CDC27, AT-hook transcription factors, DOF transcription factors, and Cycline Dependent Kinase Inhibitors (CKIs).

BACKGROUND Seed Production Regulator (SYR)

There is a continuing need to find new genes for seed production stimulation and several approaches have been used so far, for example through manipulation of plant hormone levels (WO 03/050287), through cell cycle manipulation (WO 2005/061702 ) by manipulating genes involved in response to saline stress (WO 2004/058980) among other strategies.

SYR is a new protein that has not been characterized so far. SYR shows some homology (about 48% DNA level sequence identity, about 45% protein level) with an Arabidopsis protein called ARGOS (Hu et al, Plant Cell 15, 1951-1961, 2003; Patent 2005/0108793). Hu et al. postulated that ARGOS is a protein of exclusive function and is encoded by a single gene. The main phenotypes of ARGOS overexpression in Arabidopsis are increased leaf biomass and delayed flowering. FG-GAP

FG-GAP proteins are putative transmembrane proteins. They are characterized by the presence of one or more FG-GAP domains (Pfam accession number PFO1839) and the presence of an N-terminal signal peptide and a transmembrane domain in the C-terminal half of the protein.

One such protein, DEX1, has been isolated from Arabidopsis and has been reported to play a role during pollen development (Paxson-Sowders et al. Plant physiol. 127, 1739-1749, 2001). Mutant Dexl plants are found to be defective in standard pollen wall formation. The DEX1 gene encodes an 896 amino acid protein that is predicted to localize the plasma membrane, with residues 1 to 860 being located outside the cell, residues 880 to 895 on the cytoplasmic side of the membrane, and amino acids 861 to 879 representing a potential transmembrane domain. Twelve potential N-glycosylation sites are present in DEX1. Therefore, the protein has the potential to be strongly modified and to interact with various cell wall components. DEX1 shows the greatest sequence similarity to a V. cholerae hemolysin-like protein, whereas an approximately 200 amino acid segment of DEX1 (amino acids 439-643) also shows limited similarity to the alpha-integrin calcium binding domain. In this region are at least two sets of putative calcium binding binders that are also present in a predicted Arabidopsis calmodulin protein (AC009853). Therefore, it appears that DEX1 may be a calcium binding protein. DEXl appears to be a unique plant protein; counterparts are not present in bacteria, fungi, or animals.

The changes observed in dexl plants, as well as the predicted structure of DEX1, generate several possibilities for the role of protein in pollen wall formation (Paxson-Sowders et al., 2001):

(a) DEX1 may be a protein ligand. It may be associated with the microspore membrane and participate in coupling of either primexin or sporopolenin to the plasma membrane. Absence of microspore surface protein may result in structural changes in primexin. Numerous potential N-glycosylation sites are consistent with DEX1 coupling to the callose wall, the intin, or both.

(b) DEX1 may be a component of the primexin matrix and plays a role in the initial polymerization of primexin. Changes in Ca + 2 ion concentrations appear to be important for pollen wall synthesis; Beta-glucan synthase is activated by micromolar Ca + 2 concentrations during callose wall formation.

(c) DEX1 may be part of the crude ER and may be involved in processing and / or transport of primexin precursors to the membrane. Delayed onset and general changes in primexin are consistent with a general absence of primexin precursors. The primexin matrix is initially composed of polysaccharides, proteins, and cellulose, followed by incorporation of more resistant materials. Therefore, DEX1 may participate in forming or transporting any number of different components.

CYP90B

Brassinosteroids (BRs) are a class of plant hormones that are important for promoting plant growth, division and development. The term BR collectively refers to more than forty naturally occurring polyhydroxylated sterol derivatives with structural similarity to animal steroid hormones. Of these, brassinolide was found to be the most biologically active (for review, Clouse (2002) Brassinosteroids. The Arabidopsis Book: 1-23).

The BR biosynthetic pathway was elucidated using biochemical and mutational analyzes. BRs are synthesized through at least two branched biochemical pathways starting from the same initial precursor, campesterol (Fujioka et al. (1997) physiol Plant 100: 710-715). The verified BR biosynthesis genes were found mainly encoding cytochrome P450 monooxygenases (CYP) (Bishop and Yokota (2001) Plant Cell physiol 42: 114-120). CYP superfamily of enzymes catalyzes oxidation of many chemicals, and in the present case more specifically catalyzes essential oxidative reactions in BRs biosynthesis. One of the important steps identified is the steroid side chain hydroxylation of campestanol and 6-oxocampestanol BR intermediates to form 6-deoxocatasterone and catasterone respectively. These two parallel oxidative steps are also collectively called the initial C-22 steroid alpha-hydroxylation step (Choe et al. (1998) Plant Cell 10: 231-243). In Arabidopsis, a specific CYP enzyme, CYP90B1 or DWF4, performs this step (for general reference in nomenclature of plant CYP, Nelson et al. (2004) Plant pH ys 135: 756-772). Arabidopsis mutant plants lacking steroidal alpha hydroxylase 22 activity due to insertion of a T-DNA into the DWF4 locus showed a withered phenotype due to lack of cell prolongation (Choe et al. (1998) Plant Cell 10: 231-243 ). Biochemical feeding studies with BR biosynthesis intermediates showed that all of the above compounds rescued the phenotype, while known precursors failed to do so.

Transgenic Arabidopsis and tobacco plants, both dicotyledons, were generated, which ectopically overexpressed an Arabidopsis DWF4 genomic fragment using the cauliflower mosaic virus 35S promoter (Choe et al. (2001) Plant J 26 (6): 573 -582). Phenotypic characterization of the plants showed that the hypocotyl length, plant height at maturity, total number of branches and total number of seeds were increased in transgenic plants compared to control plants. Choe et al. found that increased seed yield was due to a higher number of seeds per plant, increased seed size being within the standard deviation range. These experiments are further described in WOOO / 47715.

U.S. Patent 6,545,200 refers to isolated nucleic acid fragments encoding sterol biosynthetic genes, and more specifically claims a nucleotide sequence encoding a polypeptide having C-8.7 sterol isomerase activity. Partial nucleotide sequences encoding DWF4 are described.

U.S. Patent 2004/0060079 refers to a method of producing a modified monocotyledonous plant having a desired trait. An example is provided in which the nucleotide sequence encoding rice DWF4 (referred to as either OsDWF4 or CYP90B2) is placed under the control of a constitutive promoter, the rice actin promoter. Fourteen of the thirty-six transgenic rice plants expressing the chimeric construct show an increased number of seeds per stake compared to untransformed control plants. According to the applicants, the yield increase in GMOs compared to wild type is due to an increase in the total number of seeds, as no significant difference is found in the "weight of 10 grains".

CDC27

Depending on use, modification of certain production characteristics may be favored over others. For example for applications such as forage or wood production, or biofuel resource, an increase in the leafy parts of a plant may be desirable, and for applications such as wheat, starch or oil production, an increase in seed parameters may be desirable. particularly desirable. Even among seed parameters, some may be favored over others, depending on the application. Various mechanisms can contribute to increased seed yield, either in the form of increased seed size or increased seed number. One such mechanism is the cell cycle.

Progression through the cell cycle is critical to the growth and development of all multicellular organisms and is crucial to cell proliferation. The major components of the cell cycle are highly conserved in yeast, mammals, and plants. The cell cycle is typically divided into the following sequential phases: G0 - Gl - S - G2 - M. DNA replication or synthesis usually occurs during S phase ("S" is for DNA synthesis) and mitotic segregation of chromosomes occurs during phase M (the "M" is for mitosis), with intervening interval phases, Gl (during which cells grow before DNA replication) and G2 (a period after DNA replication during which the cell prepares for division ). Cell division is completed after cytokinesis, the last stage of phase Μ. Cells that have come out of the cell cycle and become quiescent are said to be in the GO phase. Cells in this phase can be stimulated to maintain the cell cycle in the Gl phase. The "G" in Gl, G2 and GO represents "range". Completion of the cell cycle process allows each daughter cell during cell division to receive a complete copy of the parental genome.

Cell division is controlled by two major cell cycle events, in other words beginning of DNA synthesis and beginning of mitosis. Each transition to each of these key events is controlled by a checkpoint represented by specific protein complexes (involved in DNA replication and division). The expression of genes required for DNA synthesis at the Gl / S limit is regulated by the E2F family of transcription factors in mammals and plant cells (La Thangue, 1994; Muller et al., 2001; De Veylder et al, 2002). Cell cycle entry is regulated / triggered by an E2F / Rb complex that integrates signals and enables transcription activation of cell cycle genes. The transition between the different phases of the cell cycle, and hence progression through the cell cycle, is driven by the formation and activation of different serine / threonine heterodimeric protein kinases, commonly referred to as cyclin dependent kinases (CDKs). A prerequisite for activity of these kinases is their physical association with a specific cyclin, the timing of activation being largely dependent on cyclin expression. Cyclin binding induces conformational changes in the associative CDK N-terminal lobe and contributes to the localization and substrate specificity of the complex. Monomeric CDKs are activated when they are associated with cyclins and thus have kinase activity. Cyclin protein levels fluctuate in the cell cycle and therefore represent a major factor in determining CDK activation time. Periodic activation of these cyclin and CDK-containing complexes during cell cycle mediates the temporal regulation of cell cycle transitions (checkpoints). Mechanisms exist to ensure that DNA replication occurs only once during the cell cycle. For example, CDC16, CDC23 and CDC27 proteins are part of a high molecular weight complex known as the anaphase promoter (APC) or cyclosome complex, (see Romanowski and Madine, Trends in Cell Biology 6, 184-188, 1996, and Wuarin and Nurse, Cell 85, 785-787 (1996) The yeast complex is made up of at least eight proteins, TPR (tetratricopeptide repeat) containing proteins CDC16, CDC23 and CDC27, and five other subunits called APC1, APC2, APC4, APC5 and APC7 (Peters et al. 1996, Science 274, 1199-1201) APC directs its substrates for proteolytic degradation by catalyzing the binding of ubiquitin molecules to these substrates APC-dependent proteolysis is required for chromid separation sisters in transition from metaphase to anaphase and to final mitosis output. Among the APC substrates are the anaphase inhibitory protein Pdslp and mitotic cyclins such as cyclin B, respectively (Ciosk et al. 1998, Cell 93, 10 Cohen-Fix et al 1996, Genes Dev 10, 3081-3093; Sudakin et al. 1995, Mol Biol Cell 6, 185-198; Jorgensen et al. 1998, Mol Cell Biol 18, 468-476; Townsley and Ruderman 1998, Trends Cell Biol 8, 238-244). To become active as an ubiquitin ligase, at least CDC16, CDC23 and CDC27 need to be phosphorylated at phase M (Ollendorf and Donoghue 1997, J Biol Chem 272, 32011-32018). Activated APC persists throughout Gl of the subsequent cell cycle to prevent premature appearance of tipo-type cyclins, which would result in uncontrolled S-phase entry (Irniger and Nasmyth 1997, J Cell Sci 110, 1523-1531). It has been shown in yeast that mutations in any of at least two of the APC components, CDC16 and CDC27, can result in DNA over-replication without intervening M-phase passages (Heichman and Roberts 1996, Cell 85, 39-48). This process of nuclear DNA replication without subsequent mitosis and cell division is called DNA endoreduplication, and leads to increased cell size.

CDC16, CDC23 and CDC27 are all proteins containing tetratricopeptide repeats (TPR; 34 amino acids in length). A suggested minimum consensus sequence of the TPR motif is as follows: where X is any amino acid (Lamb et al.) (X3-W-X2-LG-X2-Y-Xg-A-X2-A-X4-P-X2). 1994, EMBO J 13, 4321-4328). Consensus residues may exhibit significant degeneracy and little or no homology is present in non-consensus residues. It is the hydrophobicity and size of the consensus waste, rather than its identity, that seems to be of importance. TPR motifs are present in a wide variety of yeast functional proteins and higher mitosis eukaryotes (including the APC protein components CDC16, CDC23 and CDC27), transcription, processing, protein importation and neurogenesis (Goebl and Yanagida 1991, Trends Biochem Sci 16, 173-177). TPR forms a helical structure; Tandem repeats are organized into a superhelical structure ideally suited as interfaces for protein recognition (Groves and Barford 1999, Curr Opin Struct Biol 9, 383-389). Within the α-helix, two amphipathic domains are usually present, one in the terminal NH2 region and the other near the terminal COOH region (Sikorski et al. 1990, Cell 60, 307-317).

CDC27 (also known as Hobbit; other names include CDC27, BimA, Nuc2 or makos) has been isolated from various organisms including Aspergillus nidulans, yeast, drosophila, human and various plants (such as Arabidopsis thaliana and Oryza sativa). The gene encoding CDC27 is present as a single copy in most genomes, but two copies can be exceptionally found within the same genome, for example in Arabidopsis thaliana. The two genes encoding CDC27 proteins were named CDC27A and CDC27B (MIPS references At3gl6320 and At2g20000 respectively). Published International Patent Application WOO1 / 02430 describes sequences of CDC27A (CDC27A1 and CDC27A2) and CDC27B. Also described herein is a truncated CDC27B amino acid sequence in which 161 amino acids are absent from the terminal NH2 region. Reference is made herein to GenBank accession number AC006081 for the CDC27B gene encoding a truncated CDC27B polypeptide in the terminal NH2 region. The document reports the terminal NH2 region as being conserved in CDC27 homologs of different origin. The CDC27 sequences mentioned in WO01 / 02430 are described as being useful for modifying endoreduplication.

DNA enduplication occurs naturally in flowering plants, for example during seed development. Endoreduplication leads to enlarged nuclei with high DNA content. It has been suggested that increased DNA content during endoreduplication may support increased gene expression during endosperm development and seed filling, since it coincides with increased enzyme activity and protein accumulation at this time (Kowles et al., (1992) Genet. Eng 14: 65-88). In cereal species, cellular endosperm stores seed reserves during the endoreduplication phase. The magnitude of DNA endoreduplication is highly correlated with fresh endosperm weight, which implies an important role of DNA endoreduplication in determining endosperm mass (Engelen-Eigles et al. (2000) Plant Cell Environ. 23: 657-663 ). In maize, for example, endosperm constitutes 70 to 90% of seed mass; Thus, factors that mediate endosperm development to a large degree also determine maize grain yield through individual seed weight. Increased enduplication is therefore typically indicative of increased seed biomass but is by no means related to increased seed number. AT-hook transcription factor

An AT-hook domain is found in polypeptides belonging to a family of transcription factors associated with Chromatin remodeling. The AT-hook motif consists of 13 or more (sometimes about 9) amino acids that participate in DNA binding and have a preference for A / T-rich regions. In Arabidopsis there are at least 34 proteins containing AT-hook domains. These proteins share homology throughout most of the sequence, with the AT-hook domain being a particularly highly conserved region.

International Patent Application WO 2005/030966 describes various plant transcription factors comprising AT-hook domains and the use of these transcription factors to produce plants having increased biomass and increased stress tolerance. The application relates to G1073 members of transcription factors and states that, "Use of specific or inducible tissue promoters mitigates undesirable morphological effects that may be associated with constitutive overexpression of Gl073 members (eg, when increased size is undesirable). . " The data provided in this application refer to dicotyledonous plants. In contrast to these instructions, it has now been found that expression in a monocotyledonous plant of a polynucleic acid encoding an AT-hook transcription factor comprising a DUF296 domain (which includes Gl073 cedium members), generates plants having little or no increase in biomass compared with suitable control plants, regardless of whether such expression is driven by a constitutive promoter or in a tissue specific manner. This suggests that instructions regarding the expression of such transcription factors in dicotyledons may not be readily applicable for monocotyledons. It has now also been found that the degree or nature of any increase in seed yield obtained is dependent on the specific tissue promoter used.

DOF transcription factors

Dof domain proteins are plant specific transcription factors with a highly conserved single-finger zinc C2-C2 DNA binding domain. Over the past decade, numerous Dof domain proteins have been identified in both monocotyledon and dicotyledonous including maize, barley, wheat, rice, tobacco, Arabidopsis, squash, potatoes, and peas. Dof domain proteins have been shown to function as transcriptional activators or repressors in various plant specific biological processes.

Cycline Dependent Kinase Inhibitors (CKT)

The ability to increase plant seed production, whether by seed number, seed biomass, seed development, seed filling or any other seed-related trait, would have many applications in agriculture, and even many non-agricultural uses as in biotechnological production of substances such as pharmaceuticals, antibodies or vaccines. One approach to increasing seed production in plants may be by modifying the inherent growth mechanisms of a plant.

The inherent growth mechanisms of a plant reside in a highly ordered sequence of events collectively known as the 'cell cycle'. Progression through the cell cycle is critical to the growth and development of all multicellular organisms and is crucial to cell proliferation. The major components of the cell cycle are highly conserved in yeast, mammals, and plants. The cell cycle is typically divided into the following sequential phases: GO - Gl - S - G2 - M. DNA replication or synthesis usually occurs during S phase ("S" is for DNA synthesis) and mitotic segregation of chromosomes occurs during phase M (the "M" is for mitosis), with intervening interval phases, Gl (during which cells grow before DNA replication) and G2 (a period after DNA replication during which the cell prepares for division ). Cell division is completed after cytokinesis, the last stage of the M phase. Cells that have left the cell cycle and become quiescent are said to be in the GO phase. Cells in this phase can be stimulated to maintain the cell cycle in the Gl phase. The "G" in Gl, G2 and GO represents "gap". Completion of the cell cycle process allows each daughter cell during cell division to receive a complete copy of the parental genome.

Cell division is controlled by two major cell cycle events, in other words beginning of DNA synthesis and beginning of mitosis. Each transition to each of these key events is controlled by a checkpoint represented by specific protein complexes (involved in DNA replication and division). Gene expression required for DNA synthesis at the Gl / S limit is regulated by the E2F family of transcription factors in mammals and plant cells (La Thangue, 1994; Muller et al., 2001; De Veylder et al., 2002) . Cell cycle entry is regulated / triggered by an E2F / Rb complex that integrates signals and enables transcription activation of cell cycle genes. The transition between the different phases of the cell cycle, and hence progression through the cell cycle, is driven by the formation and activation of different serine / threonine heterodimeric protein kinases, commonly referred to as cyclin dependent kinases (CDKs). A prerequisite for activity of these kinases is their physical association with a specific cyclin, the timing of activation being largely dependent on cyclin expression. Cyclin binding induces conformational changes in the associative CDK N-terminal lobe and contributes to the localization and substrate specificity of the complex. Monomeric CDKs are activated when they are associated with cyclins and thus have kinase activity. Cyclin protein levels typically fluctuate in the cell cycle and therefore represent a major factor in determining CDK activation timing. Periodic activation of these cyclin and CDK-containing complexes during cell cycle mediates the temporal regulation of cell cycle transitions (checkpoints).

Other factors regulating CDK activity include Cycline Dependent Kinase Inhibitors (CKIs or ICKs, KIPs, CIPs, INKs), CDK Activating Kinases (CAKs), a CDK Phosphatase (Cdc25), and a CDK Subunit (CKS) (Mironov et al. 1999, Reed 1996).

The existence of a mitotic CDK inhibitor was inferred from experiments with corn seed endosperm (Grafi and Larkins (1995) Science 269, 1262-1264). Since then, several CKIs have been identified in various plant species, such as Arabidopsis (Wang et al. (1997) Nature 386 (6624): 451-2; De Veylder et al. (2001) Plant Cell 13: 1653-1668; Lui et al. (2000) Plant J 21: 379-385), tobacco (Jasinski et al. (2002) Plant physiol 2002 130 (4): 871-82), Chenopodium rubrum (Fountain et al. (1999) Plant pH ys 120: 339) or corn (Coelho et al. (2005) Plant physiol 138: 2323-2336). The encoded proteins are characterized by a stretch of approximately 45 carboxy terminal amino acids showing homology to the cyclin / Cdk amino terminal binding domain of p21Cipl / p27Kipl / p57Kip2 animal CKIs. Outside this carboxy-terminal region, plant CKIs show little homology.

Published International Patent Application WO 2005/007829 in the name of Monsanto Technology LLC describes various isolated nucleic acid molecules encoding polypeptides having cyclin-dependent kinase inhibitor activity.

Published International Patent Applications, WO 02/28893 and WO 99/14331, both in the name of CropDesign N.V., describe various plant Cycline Dependent Kinase Inhibitors. The use of these inhibitors to increase production is mentioned in these applications. SUMMARY OF THE INVENTION

It has now surprisingly been found that increasing activity of a SYR protein and / or expression of a nucleic acid encoding a SYR protein in plants results in plants having increased seed production and or increased growth rate relative to corresponding wild type plants. It has also been surprisingly found now that SYR overexpression in mice primarily increases seed yield, whereas leaf biomass and flowering time are obviously not affected (in contrast to the major ARGOS overexpression phenotypes in Arabidopsis, which have been shown to have biomass of increased leaves and delayed flowering (Hu et al., Plant Cell 15, 1951-1961, 2003; US 2005/0108793)).

According to one embodiment of the present invention there is provided a method for increasing seed production and / or growth rate of a plant comprising increasing activity of a SYR polypeptide or homologue thereof on a plant and / or expression of a nucleic acid. encoding such a protein; and optionally selecting plants having improved growth characteristics.

Advantageously, performance of the methods of the invention insofar as they relate to SYR results in plants having a variety of improved growth characteristics, such as increased seed yield without effect on plant vegetative biomass when compared to plants of matched control plants, and a comparable life cycle to matched control plants without delay in flowering season. Further advantageously, performance of the methods according to the present invention results in plants having improved tolerance for abiotic stress relative to corresponding wild type (or other control) plants.

It has now surprisingly been found that modulating activity of an FG-GAP protein and / or expression of a nucleic acid encoding an FG-GAP protein in plants results in plants having improved growth characteristics, and in particular increased yield, relative to wild type plants. corresponding.

According to another embodiment of the present invention there is provided a method for enhancing plant growth characteristics comprising modulating activity of an FG-GAP polypeptide or homologue thereof and / or modulating expression of a nucleic acid encoding an FG-GAP polypeptide or a homologue thereof on a plant and optionally select plants having improved growth characteristics.

Advantageously, performance of the methods according to the present invention, as they relate to an FG-GAP polypeptide or homologue thereof, results in plants having a variety of improved growth characteristics, such as improved growth, improved yield, improved biomass, improved architecture, or improved cell division, each relative to corresponding wild type plants. Preferably, the improved growth characteristics comprise at least increased yield over corresponding wild type plants.

It has now surprisingly been found that increasing non-constitutive expression in a plant of a nucleic acid encoding a CYP90B polypeptide or a homologue thereof generates plants having increased production relative to appropriate control plants.

According to a further embodiment of the present invention, there is provided a method for enhancing plant yield comprising increasing non-constitutive expression in a plant of a nucleic acid encoding a CYP90B polypeptide or homologue thereof.

It has now been found that preferentially increasing expression in plant bud apical meristem tissue of a nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the terminal NH2 region of the polypeptide generates plants having increased number of seeds relative to control plants. appropriate.

The invention therefore provides a method for increasing the number of plant seeds over that of suitable control plants, comprising preferably increasing tissue expression of plant shoot apical meristem of a nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain. in the NH2 terminal region of the polypeptide.

It has now been found that preferentially enhancing expression of a polypeptide-encoding nucleic acid comprising an AT-hook domain and a DUF296 domain in endosperm tissue of a monocotyledonous plant generates plants having increased seed yield over appropriate control plants.

A further embodiment of the present invention therefore provides a method for increasing seed production in monocotyledonous plants over suitable control plants, comprising preferably increasing endosperm tissue expression of a monocotyledonous plant encoding a polypeptide domain comprising a domain. AT-hook is a DUF296 domain.

It has now been found that increasing expression in a plant of a nucleic acid encoding a DOF transcription factor polypeptide generates plants having increased production relative to appropriate control plants.

According to a further embodiment of the present invention, there is provided a method for enhancing plant production comprising increasing expression in a plant of a nucleic acid encoding a DOF transcription factor polypeptide. It has now been found that preferential reduction in expression of an endogenous CKI gene in endosperm tissue of a plant generates plants with better seed yield than seed production in plants where there is no preferential reduction in expression of an endogenous CKI gene in endosperm tissue. of plant. The present invention therefore provides a method for increasing plant seed production over suitable control plants, comprising preferably reducing expression of an endogenous CKI gene in endosperm tissue of a plant.

DETAILED DESCRIPTION OF THE INVENTION

The term "increased yield" as defined herein is taken to mean an increase in biomass (weight) of one or more parts of a plant (particularly harvestable parts) relative to corresponding wild type or other control plants whose increase in biomass can be above ground or underground. An increase in underground biomass may be due to an increase in biomass of plant parts such as tubers, rhizomes, bulbs etc. Particularly preferred is an increase in any one or more of the following: increased root biomass, increased root volume, increased root number, increased root diameter, and increased root length. The term increased production also encompasses an increase in seed production.

The term "increased seed production" as defined herein is taken to mean an increase in any or more of the following, each relative to corresponding wild type plants: (i) increased total seed production, which includes an increase in seed biomass (seed weight) and which may be an increase in seed weight per plant or on an individual seed basis; (ii) increased number of flowers ("rapiers") per panicle (iii) increased number of full seeds; (iv) increased seed size; (v) increased seed volume; (vi) increased individual seed area; (vii) increased individual seed length and / or width; (viii) increased harvest index, which is expressed as a proportion of the production of harvestable parts, such as seeds, by total biomass; (ix) increased fill rate, (which is the number of full grains divided by the total number of seeds and multiplied by 100); and (x) increased thousand seed weight (TKW), which is extrapolated from the number of full grains counted and their total weight. An increased TKW may result from increased seed size and / or seed weight. An increased TKW may result from an increase in embryo size and / or endosperm size.

Taking corn as an example, an increase in yield may be manifested as one or more of the following: an increase in the number of ears per plant, an increase in number of rows, number of seeds per row, seed weight, TKW, length / ear diameter, among others. Taking rice as an example, an increase in yield may be manifested by an increase in one or more of the following: number of panicuias per plant, number of spikelets per panicle, number of flowers per panicle, increase in seed fill rate, increase in TKW, among others. An increase in production may also result in modified architecture, or may occur as a result of modified architecture.

The improved growth characteristics obtained by carrying out the methods of the invention as they relate to the use of CDC27 result in plants having increased seed numbers. An increased seed number includes an increase in the total number of seeds and / or the number of full grains and / or an increase in the seed fill rate (which is the number of full grains divided by the total number of seeds multiplied by 100 ) each in relation to appropriate control plants, the increase of which may be per plant and / or per hectare or acre. Taking maize as an example, an increase in seed numbers is typically manifested by an increase in the number of ears per plant, an increase in number of rows, number of seeds per row, increase in seed fill rate, among others. Taking rice as an example, an increase in seed numbers is typically manifested by an increase in panicles per plant, number of spikelets per panicle, number of flowers (rapiers) per panicle (which is expressed as a proportion of the number of grains filled by the number of primary panicles), increased seed filling rate.

The invention therefore provides a method for increasing the number of plant seeds over that of suitable control plants, comprising preferably increasing tissue expression of plant shoot apical meristem of a nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain. in the NH2 terminal region of the polypeptide.

To the extent that the methods of the invention relate to SYR, preferably performance of the methods results in plants having increased seed yield. Even more preferably, the increased seed yield comprises an increase in one or more of (full) number of grains, total seed weight, seed size, thousand seed weight, fill rate and crop index, each relative to plants. of control. Therefore, according to the present invention there is provided a method for increasing plant seed production, which method comprises increasing activity of a SYR polypeptide and / or expression in a plant of a nucleic acid encoding a SYR polypeptide or homologue thereof.

To the extent that the methods of the invention relate to FG-GAP, preferably performance of the methods results in plants having increased yield and, more particularly, increased biomass and / or increased seed yield. Preferably, the increased seed yield comprises an increase in one or more of (full) grain number, total seed weight, seed size, thousand seed weight and harvest index, each relative to control plants. Therefore, according to the present invention there is provided a method for increasing crop yield, particularly increased biomass and / or increased seed yield, which method comprises modulating activity of an FG-GAP polypeptide and / or expression in a plant of a nucleic acid encoding an FG-GAP polypeptide or homologue thereof.

To the extent that the methods of the invention relate to CYP90B, preferably increased yield includes one or more of the following: increased HI, increased TKW, increased seed area and increased seed length, each relative to suitable control plants. Therefore, according to the present invention there is provided a method for increasing plant yield, particularly seed yield, relative to suitable control plants, which method comprises increasing non-constitutive expression in a plant of a nucleic acid encoding a CYP90B polypeptide or a counterpart of this.

As the methods of the invention relate to AT-hook transcription factors, seed production in monocotyledonous plants is increased. Therefore, a method for increasing seed production in monocotyledonous plants over suitable control plants is provided, preferably comprising increasing endosperm tissue expression of a monocotyledonous plant of a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain. .

To the extent that the methods of the invention relate to DOF transcription factors, preferably increased yield is increased seed yield. According to a preferred feature of the present invention, there is provided a method for increasing plant seed production over suitable control plant seed production, the method of which comprises increasing expression in a plant of a nucleic acid encoding a factor polypeptide. DOF transcript.

To the extent that the methods of the invention relate to CKIs, the improved growth characteristic is increased seed yield. The present invention therefore provides a method for increasing plant seed production over suitable control plants, comprising preferably reducing expression of an endogenous CKI gene in endosperm tissue of a plant.

Since the improved plants according to the present invention have increased yield (seed yield), it is likely that these plants will exhibit increased growth rate (over at least part of their life cycle) relative to the growth rate. corresponding wild-type plants at a corresponding stage in their life cycle. The increased growth rate may be specific to one or more parts or cell types of a plant (including seeds), or may be throughout the entire plant. Plants having an increased growth rate may have a shorter life cycle. The life cycle of a plant is adopted to mean the time required to grow from a dry mature seed to the stage where the plant produced dry mature seeds, similar to the initial material. This life cycle can be influenced by factors such as early vigor, growth rate, flowering time and seed maturation speed. An increase in growth rate can occur at one or more stages in a plant's life cycle or during substantially the entire plant life cycle. Increased growth rate during the early stages of a plant's life cycle may reflect stimulated vigor. Increasing growth rate can alter a plant's harvest cycle by allowing plants to be sown later and / or harvested earlier than otherwise possible. If the growth rate is sufficiently increased, it can allow additional seeds to be sown from the same plant species (eg sowing and harvesting rice plants followed by sowing and harvesting additional rice plants all within a conventional growing period). ). Similarly, if the growth rate is sufficiently increased, it may allow additional sowing of seeds of different plant species (for example sowing and harvesting of rice plants followed by, for example, optional sowing and harvesting of soybeans, potatoes or any other suitable plant). Additional harvest times from the same rootstock for some plants may also be possible. Changing the harvest cycle of a plant can lead to an increase in annual biomass production per acre (due to an increase in the number of times (say in a year) that any particular plant can be grown and harvested). An increase in growth rate may also allow the cultivation of transgenic plants in a larger geographical area than their wild type counterparts, as the territorial limitations to growing a crop are often determined by adverse environmental conditions or at the time of planting. early) or at the time of harvest (late cycle). Such adverse conditions can be avoided if the harvest cycle is shortened. Growth rate can be determined by deriving various parameters from growth curves by plotting growth experiments, such parameters can be: T-Mid (the time taken by plants to reach 50% of their maximum size) and T-90 (time taken by plants to reach 90% of their maximum size), among others. The term "flowering time" as used herein shall mean the time period between the onset of seed germination and the onset of flowering.

Performance of the methods of the invention generates plants having an increased growth rate. Therefore, according to the present invention there is provided a method for increasing plant growth rate, which method comprises increasing activity on a plant of a SYR polypeptide or homologue thereof and / or expression of a nucleic acid encoding such a protein. .

According to the present invention there is provided a method for increasing plant growth rate, which method comprises modulating (preferably increasing) activity on a plant of an FG-GAP polypeptide or a homologue thereof and / or modulating (preferably increasing) expression of a nucleic acid encoding such a protein.

In accordance with the present invention, there is provided a method for increasing plant growth rate which method comprises increasing non-constitutive expression in a plant of a nucleic acid encoding a CYP90B polypeptide or homologue thereof.

In accordance with the present invention there is provided a method for increasing plant growth rate, which method comprises increasing expression in a plant of a nucleic acid encoding a DOF transcription factor polypeptide.

According to the present invention, there is provided a method for increasing the plant growth rate relative to suitable control plants, which method comprises preferably reducing expression of an endogenous Cyclin Dependent Kinase (CKI) Inhibitor gene in human tissue. endosperm of a plant.

An increase in seed yield and / or seed yield and / or growth rate occurs if the plant is under stress free conditions or if the plant is exposed to various stresses compared to control plants. Plants typically respond to more slowly growing stress exposure. Under conditions of severe stress, the plant may even stop growing in general. Mild stress on the other hand is defined here as any stress to which a plant is exposed that does not result in the plant stopping to grow overall without the ability to restart growth. Mild stress in the sense of the invention leads to a reduction in stressed plant growth of less than 40%, 35% or 30%, preferably less than 25%, 20% or 15%, more preferably less than 14%. %, 12%, 11% or 10% or less compared to the control plant under stress free conditions. Due to advances in agricultural practices (irrigation, fertilization, pesticide treatments) severe stresses are not often encountered in cultivated cultivars. As a consequence, compromised growth induced by mild stress is often an undesirable feature for agriculture. Mild stresses are the typical stresses to which a plant can be exposed. These stresses can be the everyday biotic and / or abiotic (environmental) stresses to which a plant is exposed. Typical abiotic or environmental stresses include temperature stresses caused by atypical heat or cold / freezing temperatures; salt stress; water stress (drought or excess water), anaerobic stress, chemical toxicity and oxidative stress. Abiotic stress can be osmotic stress caused by water stress (particularly due to drought), saline stress, oxidative stress, or ionic stress. Chemicals can also cause abiotic stresses (eg very high or very low concentrations of minerals or nutrients). Biotic stresses are typically those caused by pathogens such as bacteria, viruses, fungi and insects. The term "stress free conditions" as used herein are those environmental conditions that do not go significantly beyond the daily climatic conditions and other abiotic conditions that plants may encounter, and which allow optimal plant growth. People skilled in the art are aware of normal soil conditions and weather conditions for a given geographical location.

To the extent that the methods of the invention relate to SYR3 performance of the methods results in plants having increased tolerance for abiotic stress. As reported in Wang et al. (Planta (2003) 218: 1-14), abiotic stress leads to a series of morphological, physiological, biochemical and molecular changes that adversely affect plant growth and yield. Drought, salinity, extreme temperatures and oxidative stress are known to be interconnected and can induce cell growth and damage through similar mechanisms. For example, drought and / or salinization are primarily manifested as osmotic stress, resulting in disruption of homeostasis and ionic distribution in the cell. Oxidative stress, which often accompanies high or low temperature, salinity or drought stress can cause functional and structural protein denaturation. As a consequence, these various environmental stresses often activate cellular signaling pathways and similar cellular responses, such as stress protein production, increased antioxidants, compatible solute accumulation, and growth arrest.

Since several environmental stresses activate similar pathways, the exemplification of the present invention with drought stress (insofar as the invention relates to the use of SYR polypeptides and their coding nucleic acids) should not be construed as limiting stress by dry, but more as a separation to indicate the involvement of SYR polypeptides or homologues thereof in general abiotic stresses. In addition, the methods of the present invention may be performed under stress free conditions or under mild dry conditions to generate plants having improved growth characteristics (particularly increased yield) over corresponding wild type or other control plants.

A particularly high degree of "interference" is reported between drought stress and high salinity stress (Rabbani et al. (2003) Plant physiol 133: 1755-1767). Therefore, it would be apparent that a SYR polypeptide or a counterpart thereof, along with its usefulness in conferring drought tolerance on plants, would also find use to protect the plant against various other abiotic stresses. Similarly, it would be apparent that a SYR protein (as defined herein), along with its utility for conferring salt tolerance on plants, would also find use to protect the plant against various other abiotic stresses. In addition, Rabbani et al. (2003, Plant physiol 133: 1755-1767) report that molecular stress tolerance mechanisms and similar responses exist between dicotyledons and monocotyledons. The methods of the invention are therefore advantageously applicable to any plant.

The term "abiotic stress" as defined herein is used to mean any or more of: water stress (due to drought or excess water), anaerobic stress, saline stress, temperature stress (due to heat, cold or freezing temperatures). ), chemical toxicity stress and oxidative stress. According to one aspect of the invention, abiotic stress is an osmotic stress, selected from water stress, saline stress, oxidative stress, and ionic stress. Preferably, water stress is drought stress. The term salt stress is not restricted to common salt (NaCl), but can be any or more of: NaCl, KCl, LiCl, MgCl2, CaCl2, among others.

Increased tolerance for abiotic stress is manifested by increased crop yield under abiotic stress conditions. To the extent that the invention relates to the use of SYR polypeptides and their encoding nucleic acids, such increased yield may include one or more of the following: increased number of full seeds, increased total seed production, increased number of flowers per panicle, increased seed filling rate, increased harvest index, increased one thousand seed weight, increased root length or increased root diameter, each relative to corresponding wild type plants.

Performance of the methods of the invention generates plants having increased tolerance for abiotic stress. Performance of the methods of the invention generates plants grown under stress free conditions or under mild drought conditions with improved growth characteristics (particularly increased yield and / or increased emergency vigor (or early vigor)) over corresponding wild type plants or other plants. grown under comparable conditions.

In accordance with the present invention, there is provided a method for increasing abiotic stress tolerance in plants whose method comprises modulating expression in a plant of a nucleic acid encoding a SYR polypeptide or homologue thereof. According to one aspect of the invention, abiotic stress is osmotic stress, selected from one or more of the following: water stress, saline stress, oxidative stress, and ionic stress. Preferably, water stress is drought stress.

The present invention also provides a method for improving abiotic stress tolerance in plants, comprising increasing plant activity of a SYR protein or a homologue thereof.

To the extent that the methods of the invention relate to DOF transcription factors, the methods may be performed under mildly dry conditions to generate plants having increased yields over suitable control plants. As reported in Wang et al. (Planta (2003) 218: 1-14), abiotic stress leads to a series of morphological, physiological, biochemical and molecular changes that adversely affect plant growth and yield. Drought, salinity, extreme temperatures and oxidative stress are known to be interconnected and can induce cell growth and damage through similar mechanisms. Rabbani et al. (Plant physiol (2003) 133: 1755-1767) describes a particularly high degree of "interference" between drought stress and high salinity stress. For example, drought and / or salinization are primarily manifested as osmotic stress, resulting in disruption of homeostasis and ionic distribution in the cell. Oxidative stress, which often accompanies high or low temperature, salinity or drought stress, can cause functional and structural protein denaturation. As a consequence, these various environmental stresses often activate cellular signaling pathways and similar cellular responses, such as stress protein production, increased antioxidants, compatible solute accumulation, and growth arrest.

Performance of the methods of the invention generates plants grown under mild dry conditions with increased yield over suitable control plants grown under comparable conditions. Therefore, according to the present invention there is provided a method for increasing production in plants grown under mild dry conditions, which method comprises increasing expression in a plant of a nucleic acid encoding a DOF transcription factor polypeptide.

The improved growth characteristics mentioned above can advantageously be improved on any plant. To the extent that the methods of the invention relate to the use of AT-hook transcription factors, the methods are applicable to monocotyledonous plants.

The term "plant" as used herein encompasses whole plants, ancestors and progeny of plants and plant parts, including seeds, buds, stems, leaves, roots (including tubers), flowers, and tissues and organs, characterized by the fact that each one of the above mentioned comprises the gene / nucleic acid of interest or the genetic modification in the gene / nucleic acid of interest. The term "plant" also encompasses plant cells, suspension crops, callus tissue, embryos, meristematic regions, gametophytes, sporophytes, pollen and microspores, again characterized by the fact that each of the above comprises the gene / nucleic acid of interest.

Plants which are particularly useful in the methods of the invention include all plants belonging to the Viridiplantae superfamily, in particular monocotyledonous and dicotyledonous plants including fodder or forage vegetables, ornamental plants, food crops, trees or shrubs selected from the list comprising Acer spp. , Actinidia spp., Abelmoschus spp., Agropyron spp., Allium spp., Amaranthus spp., Ananas eomosus, Annona spp., Apium graveolens, Araehis spp, Artoearpus spp., Asparagus officinalis, Avena spp. (e.g. Avena sativa, Avena fatua, Avena byzantina, Avena fatua var. sativa, Avena hybrida), Averrhoa earambola, Benineasa hispida, Bertholletia exeelsea, Beta vulgaris, Brassiea spp. (eg Brassiea napus, Brassiea rapa ssp. [canola, rapeseed, turnip]), Cadaba farinosa, Camellia sinensis, Canna indica, Capsicum spp. Carex elata, Caricapapaya, Carissa maerocarpa, Carya spp., Carthamus tinctorius, Castanea spp. Cichorium endivia, Cinnamomum spp., Citrullus lanatus, Citrus spp., Cocos spp., Coffea spp., Colocasia esculenta, Cola spp., Coriandrum sativum, Corylus spp., Crataegus spp., Crocus sativus, Cucurbita spp. Cynara spp., Daucus earota, Desmodium spp., Dimocarpus longan, Dioscorea spp., Diospyros spp., Echinochloa spp., Elaeis (eg Elaeis guineensis, Elaeis oleifera), Eleusine korea Fagus spp., Ficus carica, Fortunella spp., Fragaria spp., Ginkgo biloba, Glycine spp. (e.g. Glycine max, Soya hispida or Soy max), Gossypium hirsutum, Helianthus spp. (e.g. Helianthus annuus), Hemerocallis fulva, Hibiscus spp., Hordeum spp. (e.g. Hordeum vulgaré), Ipomoea potatoes, Juglans spp., Lactuca sativa, Lathyrus spp., Lens culinaris, Linum usitatissimum, Litehi chinensis, Lotus spp., Luffa acutangula, Lupinus spp., Luzula sylvatica, Lyeopersieon spp. (eg Lycopersicon esculentum, Lyeopersieon lycopersicum, Lyeopersieon pyriformé), Macrotyloma spp., Malus spp., Malpighia emarginata, Mammea americana, Maniif spp., Manilhotara spp. , Morus nigra, Musa spp., Nicotiana spp., Olea spp., Opuntia spp., Ornithopus spp., Oryza spp. (eg Oryza sativa, Oryza latifolia), Panicum miliaceum, Passiflora edulis, Pastinaea sativa, Persea spp., Petroselinum crispum, pH aseolus spp., pH ysalis spp., Pinus spp., Pistaeia vera, Pisum spp. , Poa spp., Populus spp., Prosopis spp., Prunus spp., Psidium spp., Punica granatum, Pyrus communis, Quereus spp., Raphanus sativus, Rheum rhabarbarum, Ribes spp., Ricinus communis, Rubus spp., Saecharum spp. ., Sambucus spp., Secale cereale, Sesamum spp., Sinapis sp., Solanum spp. (e.g. Solanum tuberosum, Solanum integrifolium or Solanum lycopersieum), Sorghum bicolor, Spinacia spp., Syzygium spp., Tagetes spp., Tamarindus indica, Theobroma cacao, Trifolium spp., Triticosecale rimpaui, Triticum spp. (eg Triticum aestivum, Triticum durum, Triticum turgidum, Triticum hybernum, Triticum macha, Triticum sativum or Triticum vulgaré), Tropaeolum minus, Tropaeolum majus, Vaccinium spp., Vicia spp. Zea mays, Zizania palustris, Ziziphus spp., Among others.

Preferably, the plant is a cultivable plant such as soybean, sunflower, canola, alfalfa, rapeseed, cotton, tomato, potato or tobacco. Even more preferably, the plant is a monocot plant, such as sugar cane. More preferably the plant is a cereal, such as rice, corn, wheat, barley, millet, rye, sorghum or oats.

Where the methods of the invention relate to the use of an AT-hook transcription factor, the monocot plant is a cereal such as rice, corn, sugar cane, wheat, barley, millet, rye, sorghum, grass or oats. .

DEFINITIONS

Polypeptide The terms "polypeptide" and "protein" are used interchangeably herein and refer to amino acids in a polymeric form of any length. The terms "polynucleotide (s)", "nucleic acid sequence (s)", "nucleotide sequence (s)" are used interchangeably herein and refer to nucleotides, or ribonucleotides or deoxyribonucleotides or a combination of both, in a polymeric form of any length.

Control Plant

The choice of suitable control plants is a routine part of an experimental structure and may include corresponding wild type plants or corresponding plants without the gene of interest. The control plant is typically of the same plant species or even the same variety as the plant to be verified. The control plant can also be a nullizigote of the plant to be verified. A "control plant" as used herein refers not only to whole plants, but also to plant parts, including seeds and parts of seeds.

Increase, Improve

The terms "increase", "improving" or "improvement" are used interchangeably here and are adopted to mean at least 5%, 6%, 7%, 8%, 9% or 10%, preferably at least 15% or 20%, more preferably 25%, 30%, 35% or 40% more production and / or growth compared to corresponding wild type or other control plants as defined herein.

Hybridization

The term "hybridization" as defined herein is a process characterized by the fact that substantially homologous complementary nucleotide sequences anneal each other. The hybridization process may occur entirely in solution, i.e. both complementary nucleic acids are in solution. The hybridization process may also occur with one of the complementary nucleic acids immobilized to a matrix such as magnetic microspheres, Sepharose microspheres or any other resin. The hybridization process may further occur with one of the complementary nucleic acids immobilized to a solid support such as nitrocellulose or nylon membrane or immobilized by eg photolithography to, for example, a silicon glass support (the latter known as arrays or microarrays). nucleic acid or as nucleic acid integrated circuits). In order to allow hybridization to occur, nucleic acid molecules are generally thermally or chemically denatured to melt a double strand into two single strands and / or to remove staple structures or other secondary strands of single stranded nucleic acids. Hybridization stringency is influenced by conditions such as temperature, salt concentration, ionic strength and hybridization buffer composition.

"Stringent hybridization conditions" and "Stringent hybridization wash conditions" in the context of nucleic acid hybridization experiments such as Southern and Northern hybridizations are sequence dependent and are different in different environmental parameters. The skilled artisan is aware of various parameters which may be changed during hybridization and washing and which will either maintain or change stringency conditions.

Tm is the set ion temperature and pH at which 50% of the target sequence hybridizes to a perfectly matched probe. Tm is dependent on solution conditions and ad base composition and probe length. For example, larger sequences hybridize specifically at higher temperatures. The maximum hybridization rate is obtained from about 16 ° C to 32 ° C below Tm. The presence of monovalent cations in the hybridization solution reduces electrostatic repulsion between the two nucleic acid strands thereby promoting hybrid formation; This effect is visible at sodium concentrations up to 0.4M. Formamide reduces the fusion temperature of DNA-DNA and DNA-RNA duplexes by 0.6 to 0.7 ° C for each percent formamide, and addition of 50% formamide allows hybridization to be performed at 30 to 45 ° C, although the hybridization rate is decreased. Incorrect base pairing reduces the hybridization rate and thermal stability of the duplexes. On average and for large probes, Tm decreases by about 1 ° C by% mismatch. Tm can be calculated using the following equations, depending on the types of hybrids:

• DNA-DNA Hybrids (Meinkoth and Wahl, Anal. Biochem., 138: 267-284, 1984):

Tm = 81.5 ° C + 16.6xlog [Na +] at + 0.41x% [G / C "b] -500x [L" c] -1-0.61x% formamide

• DNA-RNA or RNA-RNA hybrids:

Tm = 79.8 + 18.5 (log10 [Na +] a) +0.58 (% G / C "b) + 11.8 (% G / C" b) 2-820 / L "c

• Oligo-DNA or oligo-RNA "d hybrids:

For <20 nucleotides: Tm = 2 (ln)

For 20-35 nucleotides: Tm = 22 + 1.46 (ln) a or for another monovalent cation, but only accurate in the range 0.01-0.4 M.

b only accurate for% GC in the range of 30% to 75%.

c L = base pair duplex length.

d Oligo, oligonucleotide; ln, effective primer length = (G / C no.) + (A / T no.).

Note: for every 1% formamide, Tm is reduced by about 0.6 to 0.7 ° C, while the presence of 6M urea reduces Tm by about 30 ° C.

Hybridization specificity is typically the function of posthybridization washes. To remove background resulting from nonspecific hybridization, samples are washed with dilute saline. Critical factors of such washes include the ionic strength and temperature of the final wash: the lower the salt concentration and the higher the wash temperature, the greater the stringency of the wash. Washing conditions are typically performed at or below hybridization stringency. Generally, stringent conditions suitable for nucleic acid hybridization assays or gene amplification detection procedures are as set forth above. More or less stringent conditions may also be selected. Generally, low stringency conditions are selected to be about 50 ° C lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH. Medium stringency conditions are when the temperature is 20 ° C below Tm, and high stringency conditions are when the temperature is 10 ° C below Tm. For example, stringent conditions are those that are at least as stringent as, for example, conditions A-L; and reduced stringency conditions are at least as stringent as, for example, M-R conditions. Non-specific binding can be controlled using any of several known techniques such as, for example, blocking the membrane with protein-containing solutions, RNA additions, heterologous DNA, and SDS to the hybridization buffer, and Rnase treatment.

Examples of hybridization and wash conditions are listed in Table 1:

Table 1:

<table> table see original document page 39 </column> </row> <table> <table> table see original document page 40 </column> </row> <table>

* "Hybrid Length" is the anticipated length for hybridizing nucleic acid. When nucleic acids of known sequence are hybridized, the hybrid length can be determined by aligning the sequences and identifying the conserved regions described herein.

+ SSPE (1xSSPE is 0.15M NaCl, 10mM NaH2PO4, 1, 25mM EDTA, pH 7.4) can be replaced by SSC (1 * SSC is 0.15M NaCl and 15mM sodium citrate) in buffers. hybridization and washing; washes are performed for 15 minutes after hybridization is complete. Hybridizations and washes may additionally include 5 χ Denhardt reagent, 0.5-1.0% SDS, 100 μg / ml shredded salmon sperm DNA, 0.5% sodium pyrophosphate, and up to 50 % formamide.

* Tb-Tr: The hybridization temperature anticipated for hybrids to be less than 50 base pairs in length must be 5-10 ° C lower than the melt temperature Tm of hybrids; Tm is determined according to the equations mentioned above.

The present invention also encompasses the replacement of any one or more DNA or RNA hybrid partners or a PNA, or a modified nucleic acid.

For purposes of defining the stringency level, reference may conveniently be made to Sambrook et al. (2001) Molecular Cloning: a laboratory manual, 3rd Edition Cold Spring Harbor Laboratory Press, CSH, New York or to Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989).

T-DNA Activation Identification

T-DNA activation identification (Hayashi et al. Science (1992) 1350-1353) involves insertion of T-DNA, usually containing a promoter (may also be a translation enhancer or intron), into the genomic region of the interest or 10 kb before or after the coding region of a gene in a configuration such that the promoter drives expression of the directed gene. Typically, expression regulation of the gene driven by its natural promoter is disrupted and the gene falls under the control of the newly introduced promoter. The promoter is typically embedded in a T-DNA. This T-DNA is randomly inserted into the plant genome, for example through Agrobacterium infection and leads to overexpression of genes near the inserted T-DNA. The resulting transgenic plants show dominant phenotypes due to overexpression of genes near the introduced promoter. The promoter to be introduced may be any promoter capable of directing expression of a gene in the desired organism, in this case a plant. For example, constitutive, tissue preferred, cell type preferred and inducible promoters are all suitable for use in T-DNA activation. TILLING

TILLING is a mutagenesis technology useful for generating and / or identifying and / or eventually isolating mutagenized variant nucleic acids. TILLING also allows selection of plants carrying such mutant variants. These mutant variants may still exhibit greater activity than that exhibited by the gene in its natural form. TILLING combines high density mutagenesis with rapid screening methods. The steps typically followed in TILLING are: (a) EMS mutagenesis (Redei GP and Koncz C (1992) In Methods in Arabidopsis Research, Koncz C, Chua NH, Schell J, eds. Singapore, World Scientific Publishing Co, pp. 16- 82; Feldmann et al. (1994) In Meyerowitz EM, Somerville CR, eds, Arabidopsis Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 137-172; Lightner J and Caspar T (1998) In J Martinez- Zapater, J Salinas, eds, Methods on Molecular Biology, Vol. 82. Humana Press, Totowa, NJ, pp 91-104); (b) DNA preparation and combination of individuals; (c) PCR amplification of a region of interest; (d) denaturation and annealing to allow heteroduplex formation; (e) DHPLC, where the presence of a heteroduplex in a combination is detected as an extra peak in the chromatogram; (f) identification of the mutant individual; and (g) sequencing of the mutant PCR product. Methods for TLLLINiG are well known in the art (McCallum et al., (2000) Nat Biotechnol 18: 455-457; reviewed by Stemple (2004) Nat Rev Genet 5 (2): 145-50).

Site directed mutagenesis

Site directed mutagenesis can be used to generate SYR nucleic acid variants. Several methods are available to achieve site directed mutagenesis; the most common being PCR-based methods (Current Protocols in Molecular Biology. Wiley Eds. http://www.4ulr.com/products/currentprotocols/index.html).

Transposon mutagenesis

Transposon mutagenesis is a mutagenesis technique based on the insertion of transposons in genes, which often results in gene knockout. The technique has been used for several plant species, including rice (Greco et al., Plant physiol, 125, 1175-1177, 2001), corn (McCarty et al., Plant J. 44, 52-61, 2005) and Arabidopsis. (Parinov and Sundaresan, Curr. Opin. Biotechnol. 11, 157-161, 2000).

Direct evolution

Direct gene evolution or shuffling consists of DNA scrambling repeats followed by appropriate screening and / or selection to generate variant nucleic acids or portions thereof, or polypeptides or homologues thereof having a modified biological activity (Castle et al., (2004) Science 304 (5674): 1151-4; U.S. Patent Nos. 5,811,238 and 6,395,547).

Year Recombination

Homologous recombination allows introduction into a genome of a selected nucleic acid at a defined selected position. Homologous recombination is a standard technology routinely used in the life sciences for lower organisms such as yeast or pH yscomitrella moss. Methods for performing homologous recombination on plants have been described not only for model plants (Offringa et al. (1990) EMBO J 9 (10): 3077-84) but also for cultivars, for example rice (Terada et al. (2002) Nat. Biotech 20 (10): 1030-4; Iida and Terada (2004) Curr Opin Biotech 15 (2): 132-8). The nucleic acid to be targeted (which may be any of the nucleic acids or variants defined herein) must be directed to the particular gene locus. The nucleic acid to be targeted may be an improved allele used to replace the endogenous gene or may be introduced in addition to the endogenous gene.

Counterparts

"Protein homologues" include peptides, oligopeptides, polypeptides, proteins and enzymes having amino acid substitutions, deletions and / or insertions with respect to the unmodified protein in question and having similar biological and functional activity to the unmodified protein from which they are derived. . To produce such homologues, amino acids of the protein may be substituted for other amino acids having similar properties (such as similar hydrophobicity, hydrophilicity, antigenicity, propensity to form or break α-helical structures or β-leaf structures). Conservative substitution tables are well known in the art (see for example Creighton (1984) Proteins. W.H. Freeman and Company and Table 2 below).

Orthologists and Paralogues

Covered by the term "homologues" are orthologous sequences and paralog sequences, two special forms of homology that encompass evolutionary concepts used to describe ancestral gene relationships.

The term "paralogs" refers to gene duplications within the genome of a species leading to paralog genes. Paralogues can be easily identified by performing a BLAST analysis against a set of sequences of the same kind as the query sequence.

The term "orthologs" refers to homologous genes in different organisms due to speciation. Orthologists in, for example, dicotyledonous plant species can be easily found by performing a so-called reciprocal blast search. This can be done by a first blast involving overloading a query string (for example, SEQ ID NO: 1 or SEQ ID NO: 2) against any sequence database, such as the publicly available NCBI database that can be found at: http://www.ncbi.nlm.nih.gov. BLASTN or TBLASTX (using default default values) can be used when starting from a nucleotide sequence and BLASTP or TBLASTN (using default default values) can be used when starting from a protein sequence. BLAST results can optionally be filtered. Full length sequences of either filtered or unfiltered results are then BLASTed back (according to BLAST) against sequences from the organism from which the query sequence is derived (where the query sequence is SEQ ID NO: 1 or SEQ ID NO : 2 the second blast would therefore be against Oryza sativa sequences). The results of the first and second BLASTs are then compared. A paralog is identified if a high-ranking second blast hit is of the same kind from which the query sequence is derived; An ortholog is identified if a high ranking hit is not of the same kind from which the query sequence is derived. High ranking hits are those with a low E value. The lower the E value, the more significant the score (or in other words, the lower the chance that the hit will be found at random). E-value computing is well known in the art. For large families, ClustalW can be used, followed by a neighboring tree to help visualize related gene grouping and to identify orthologs and paralogues.

A homolog may be in the form of a "substitutional variant" of a protein, i.e. where at least one residue in an amino acid sequence has been removed and a different residue inserted in its place. Amino acid substitutions typically are from single residues, but may be grouped depending on functional restrictions placed on the polypeptide; Insertions will usually be on the order of about 1 to 10 amino acid residues. Preferably, amino acid substitutions comprise conservative amino acid substitutions. Less conservative substitutions may be made in case the amino acid properties mentioned above are not so critical. Conservative replacement tables are readily available in the art. The Table below provides examples of conservative amino acid substitutions.

Table 2: Examples of conserved amino acid substitutions:

<table> table see original document page 45 </column> </row> <table>

A homolog may also be in the form of an "insertional variant" of a protein, i.e. where one or more amino acid residues are introduced at a predetermined site in a protein. Insertions may comprise N-terminal and / or C-terminal fusions as well as single or multiple intra-sequence amino acid insertions. Generally, insertions within the amino acid sequence will be smaller than N- or C-terminal fusions of the order of about 1 to 10 residues. Examples of N- or C-terminal fusion proteins or peptides include the binding domain or activation domain of a transcriptional activator as used in the yeast two hybrid system, phage coat proteins, (histidine) -6 label, glutathione S-transferase tag, protein A, maltose binding protein, dihydrofolate reductase, epitope Tag · 100, c-myc epitope, FLAG® epitope, IacZ, CMP (calmodulin binding peptide), HA epitope, protein C epitope and VSV epitope.

Homologs in the form of "deletion variants" of a protein are characterized by the removal of one or more amino acids from a protein.

Amino acid variants of a protein can be readily made using synthetic peptide techniques well known in the art, such as solid phase peptide synthesis and the like, or by recombinant DNA manipulations. Methods for manipulating DNA sequences to produce substitution, insertion, or deletion variants of a protein are well known in the art. For example, techniques for making substitution mutations at predetermined DNA sites are well known to those skilled in the art and include M13 mutagenesis, T7-Gen in vitro mutagenesis (USB, Cleveland, OH), QuickChange site-directed mutagenesis (Stratagene, San Diego, CA), PCR-mediated site-directed mutagenesis or other site-directed mutagenesis protocols.

Derivatives

"Derivatives" are polypeptides or proteins which may comprise naturally modified and / or unnaturally modified amino acid residues compared to the amino acid sequence in a naturally occurring (i.e. not post-translationally modified) form of the protein, for example, as set forth in SEQ ID NO: 2. "Derivatives" of a protein encompass polypeptides or proteins which may comprise altered naturally occurring amino acid residues, glycosylated, acylated, prenylated or non-naturally occurring amino acid residues compared to the amino acid sequence of one form. naturally occuring of the polypeptide. A derivative may also comprise one or more non-amino acid substituents compared to the amino acid sequence from which it is derived, for example a reporter molecule or other linker, covalently or non-covalently linked to the amino acid sequence, such as a reporter molecule that is linked. for ease of detection, and unnaturally occurring amino acid residues relative to the amino acid sequence of a non-naturally occurring protein.

Alternative union variants

The term "alternative joining variant" as used herein encompasses variants of a nucleic acid sequence in which selected introns and / or exons have been cut, substituted or added, or in which introns have been shortened or lengthened. Such variants will be ones in which the biological activity of the protein is maintained, which can be achieved by selectively maintaining functional protein segments. Such union variants may be found in nature or may be man-made. Methods for making such joint variants are known in the art.

Allelic variant

Allelic variants exist in nature, and encompassed within the methods of the present invention is the use of these natural alleles. Allelic variants include Single Nucleotide Polymorphisms (SNPs) as well as Small Insert / Deletion Polymorphisms (INDELs). The size of INDELs is typically less than 100 bp. SNPs and INDELs form the largest set of sequence variants in naturally occurring polymorphic strains of most organisms.

District Attorney

The terms "regulatory element", "control sequence" and "promoter" are all used interchangeably herein and should be adopted in a broad context to refer to nucleic acid regulatory sequences capable of expressing the sequences to which they are linked. . Covered by the terms mentioned above are transcriptional regulatory sequences derived from a classic eukaryotic genomic gene (including the TATA box that is required for accurate transcription initiation, with or without a CCAAT box sequence) and additional regulatory elements (ie sequences above the promoters). stimulants and silencers) that alter gene expression in response to development and / or external stimuli, or in a specific tissue manner. Also included within the term is a transcriptional regulatory sequence of a classic prokaryotic gene, in which case it may include a -35 box sequence and / or additional -10 box regulatory sequences. The term "regulatory element" also encompasses a synthetic or derivative fusion molecule that confers, activates or stimulates expression of a nucleic acid molecule in a cell, tissue or organ. The term "operably linked" as used herein refers to a functional link between the promoter sequence and the gene of interest, such that the promoter sequence is capable of initiating transcription of the gene of interest.

The promoter may be an inducible promoter, i.e. having induced or enhanced transcription initiation in response to a development, chemical, environmental or physical stimulus.

A preferred tissue or tissue specific promoter is one that is capable of preferentially initiating transcription in certain tissues, such as leaves, roots, seed tissue etc, or even specific cells.

The term "constitutive" as defined herein refers to a promoter that is predominantly expressed in at least one tissue or organ and predominantly at any stage of plant life. Preferably the promoter is expressed predominantly throughout the plant.

Examples of other constitutive promoters are shown in Table 3 below.

Table 3: Examples of constitutive promoters

<table> table see original document page 49 </column> </row> <table>

Table 4: Examples of non-constitutive promoters

<table> table see original document page 49 </column> </row> <table> <table> table see original document page 50 </column> </row> <table>

Table 5: Examples of early sprout apical meristem promoters

<table> table see original document page 50 </column> </row> <table>

Table 6: Examples of specific endosperm promoters for use in the present invention.

<table> table see original document page 50 </column> </row> <table> <table> table see original document page 51 </column> </row> <table>

Table 7: Examples of specific seed promoters for use in the present invention.

<table> table see original document page 51 </column> </row> <table> <table> table see original document page 52 </column> </row> <table>

Terminator Sequence

The term "terminator" encompasses the control sequence which is a DNA sequence at the end of a transcriptional unit that signals 3 'processing and polyadenylation of a primary transcript and transcription termination. Additional regulatory elements may include transcriptional as well as translational enhancers. Those skilled in the art will be aware of terminator and enhancer sequences that may be suitable for use in carrying out the invention. Such sequences would be known or readily obtainable by one of ordinary skill in the art.

Selectable Marker

The term "selectable marker gene" as referred to herein includes any gene that confers a phenotype on a cell in which it is expressed to facilitate identification and / or selection of cells that are transfected or transformed with a nucleic acid construct of the invention. Suitable markers can be selected from markers that confer antibiotic or herbicide resistance, introduce a new metabolic trait, or allow visual selection. Examples of selectable marker genes include genes conferring antibiotic resistance (such as npt11 which phosphorylates neomycin and kanamycin, or hpt, phosphorylating hygromycin), herbicides (e.g. bar that provides resistance to Basta ™; aroA or gox providing resistance to glyphosate), or genes that provide a metabolic trait (such as manA that allows plants to use mannose as their sole carbon source). Visual marker genes result in color formation (e.g. β-glucuronidase, GUS), luminescence (such as luciferase) or fluorescence (Green Fluorescent Protein, GFP, and derivatives thereof).

Transformation

The term "transformation" as referred to herein encompasses the transfer of an exogenous polynucleotide to a host cell, regardless of the method used for transfer. Plant tissue capable of subsequent clonal propagation, either by organogenesis or embryogenesis, can be transformed with a genetic construct of the present invention and an entire plant regenerated therefrom. The particular tissue chosen will vary depending on the clonal propagation systems available for and best suited for the particular species being transformed. Exemplary tissue targets include leaf discs, pollen, embryos, cotyledons, hypocotyls, megagametophils, callus tissue, existing meristematic tissue (eg, apical meristem, axillary buds, and root meristems), and induced meristem tissue (eg, meristem of cotyledon and hypocotyl meristem). The polynucleotide may be transiently or stably introduced into a host cell and may be kept unintegrated, for example, as a plasmid. Alternatively, it can be integrated into the genome. host The resulting transformed plant cell can then be used to regenerate a transformed plant in a manner known to those skilled in the art.

Transformation of plant species is now a fairly routine technique. Advantageously any of the various transformation methods may be used to introduce the gene of interest into a suitable ancestral cell. Transformation methods include the use of liposomes, electroporation, chemicals that increase free DNA absorption, DNA injection directly into the plant, particle gun bombardment, virus or pollen transformation, and microprojection. Methods can be selected from the calcium / polyethylene glycol method for protoplasts (Krens, F.A. et al., (1982) Nature 296, 72-74; Negrutiu I et al. (1987) Plant Mol Biol 8: 363-373); protoplast electroporation (Shillito R.D. et al. (1985) Bio / Technol 3, 1099-1102); microinjection in plant material (Crossway A et al., (1986) Mol. Gen Genet 202: 179-185); DNA or RNA coated particle bombardment (Klein TM et al., (1987) Nature 327: 70) infection with (non-integrative) viruses and the like. Transgenic rice plants are preferably produced by Agrobacterium-mediated transformation using any of the well-known rice transformation methods as described in any of the following: European Patent Application published 1198985 Al, Aldemita and Hodges (Plant 199: 612-617 , 1996); Chan et al. (Plant Mol Biol 22 (3): 491-506, 1993), Hiei et al. (Plant J 6 (2): 271-282, 1994), the descriptions of which are incorporated by reference herein as if fully exposed. In the case of corn processing, the preferred method is as described or in Ishida et al. (Nat. Biotechnol 14 (6): 745-50, 1996) or Frame et al. (Plant physiol 129 (1): 13-22, 2002), the descriptions of which are incorporated by reference herein as if fully exposed.

Generally after transformation, plant cells or cell clusters are selected for the presence of one or more markers that are encoded by expressible genes in plants co-transferred with the gene of interest, following which the transformed material is regenerated into an entire plant.

Following DNA transfer and regeneration, putatively transformed plants can be evaluated, for example using Southern analysis, for the presence of the gene of interest, copy number and / or genomic organization. Alternatively or additionally, newly introduced DNA expression levels may be monitored using Northern and / or Western analysis, both techniques being well known to those of ordinary skill in the art.

The generated transformed plants can be propagated in a variety of ways, such as by clonal propagation or classical breeding techniques. For example, a first generation (or TI) transformed plant can be vegetatively reproduced and second generation (or T2) homozygous transformants selected, and T2 plants can then be further propagated by classical breeding techniques.

Generated transformed organisms can take a variety of forms. For example, they may be chimeras of transformed cells and untransformed cells; clonal transformants (e.g., all cells transformed to contain the expression drawer); transformed and unprocessed tissue grafts (e.g., in plants, a transformed rootstock grafted to an unprocessed offspring). Detailed Description Seed Production Regulator (SYR)

The activity of a SYR protein may be increased by increasing levels of the SYR polypeptide. Alternatively, activity may also be increased when there are no changes in levels of a SYR, or even when there is a reduction in levels of a SYR protein. This can occur when the intrinsic properties of the polypeptide are altered, for example by producing a mutant or selecting a variant that is more active than wild type.

The term "SYR protein or homologue thereof" as defined herein refers to a polypeptide of about 65 to about 200 amino acids, comprising (i) a leucine-rich domain that looks like a leucine zipper on the C-terminal half of the protein. , whose leucine-rich domain is (ii) preceded by a tripeptide with the sequence YFS (conserved motif la, SEQ ID NO: 6), or YFT (conserved motif lb, SEQ ID NO: 7), or YFG (conserved motif lc , SEQ ID NO: 8) or YLG (conserved motif ld, SEQ ID NO: 9), and (iii) followed by the conserved motif 2 ((V / A / I) LAFMP (T / S), SEQ ID NO: 10). Preferably the conserved motif 2 is (A / V) LAFMP (T / S), most preferably the conserved motif is VLAFMPT. The "SYR protein or homologue thereof" preferably also has a conserved C-terminus peptide ending with conserved motif 3 (SYL or PYL5 SEQ ID NO: 11).

The leucine-rich domain of the SYR protein or homologue thereof is about 38 to 48 amino acids in length, starting just behind the conserved motif 1 and stopping just before the conserved motif 2, and comprises at least 30% leucine. The Leu-rich domain preferably has a motif resembling the Leucine Zipper motif (L-X6-L-X6-L-X6-L, characterized by the fact that X6 is a sequence of 6 consecutive amino acids). A preferred example of a SYR protein is represented by SEQ ID NO: 2, an overview of its domains is given in Figure 1. It should be noted that the term "SYR protein or homologue thereof" does not encompass Arabidopsis thaliana ARGOS protein ( SEQ ID NO: 26).

Even more preferably, SYR proteins have two transmembrane domains, with the N-terminal part and C-terminal part of the protein located within and the part between the transmembrane domains located outside.

Alternatively, the counterpart of a SYR protein has in ascending order preferably at least 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38 %, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71% 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88 %, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% total amino acid sequence identity represented by SEQ ID NO: 2 as long as the homologous protein comprises conserved motifs 1 (a, b, c or d), 2 and 3, and leucine rich domain as described above. Total sequence identity is determined using a global alignment algorithm, such as the Needleman Wunsch algorithm in the GCG Wisconsin Package, Accelrys (GAP) program, preferably with default parameters.

The various structural domains in a SYR protein can be identified using specialized databases eg SMART (Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864; Letunic et al. (2002) Nucleic Acids Res 30, 242-244; http://smart.embl-heidelberg.de/), InterPro (Mulder et al., (2003) Nucl. Acids. Res. 31, 315-318; http: //www.ebi .ac.uk / interpro /), Prosite (Bucher and Bairoch (1994), A generalized profile syntax for biomolecular sequences motifs and its funetion in automatic sequence interpretation. (In) ISMB-94; Proceedings 2nd International Conference on Intelligent Systems for Molecular Altman R, Brutlag D., Karp P., Lathrop R., Searls D., Eds., Pp53-61, AAAIPress, Menlo Park; Hulo et al., Nucl. Acids Res. 32: D134-D137, (2004), http://www.expasy.org/prosite/) or Pfam (Bateman et al., Nucleic Acids Research 30 (l): 276-280 (2002), http://www.sanger.ac. uk / Software / Pfam /).

Methods for searching and identifying SYR homologs would be well within the skill of the art. Such methods include comparison of sequences represented by SEQ ID NO: 1 or 2, in a computer readable format, with sequences that are available in public databases such as MIPS (http://mips.gsf.de/), GenBank. (http://www.ncbi.nlm.nih.gov/Genbank/index.html) or EMBL Nucleotide Sequence Database (http://www.ebi.ac.uk/embl/index.html) , using algorithms well known in the art for sequence alignment or comparison, such as GAP (Needleman and Wunsch, J. Mol. Biol. 48; 443-453 (1970)), BESTFIT (using the Smith and Smith local homology algorithm). Waterman (Advances in Applied Mathematics 2; 482-489 (1981))), BLAST (Altschul, SF, Gish, W., Miller, W., Myers, EW & Lipman, DJ, J. Mol. Biol. 215: 403 -410 (1990)), FASTA and TFASTA (WR Pearson and DJ Lipman Proc.Natl.Acad.Sci. USA 85: 2444-2448 (1988)). The computer application for performing BLAST analysis is publicly available through the National Center for Biotechnology Information (NCBI).

Transmembrane domains are about 15 to 30 amino acids in length and are usually composed of hydrophobic residues that form an alpha helix. They are usually predicted on the basis of hydrophobicity (e.g. Klein et al., Biochim. Biophys. Acta 815, 468, 1985; or Sonnhammer et al., In J. Glasgow, T. Littlejohn, F. Major, R. Lathrop, D. Sankoff, and C. Sensen, editors, Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology, pages 175-182, Menlo Park, CA, 1998. AAAI Press.).

Examples of proteins falling under the definition of "SYR polypeptide or a homologue thereof" are listed in Table A of Example 1 and include sequences from various monocot plants such as rice (SEQ ID NO: 2, SEQ ID NO: 12 and SEQ ID NO: 13), maize (SEQ ID NO: 14 and SEQ ID NO: 44), wheat (SEQ ID NO: 15), barley (SEQ ID NO: 16), sugar cane (SEQ ID NO: 17 and SEQ ID NO: 18), sorghum (SEQ ID NO: 19); and dicotyledonous plants such as Arabidopsis (SEQ ID NO: 20 and SEQ ID NO: 21), grape (SEQ ID NO: 22), citrus (SEQ ID NO: 23) or tomato (SEQ ID NO: 24 and SEQ ID NO) : 25). It is contemplated that the Leu rich domain is important for protein function, so proteins with the Leu rich domain but without the conserved motifs 1 or 2 may also be useful in the methods of the present invention; Examples of such proteins are given in SEQ ID NO: 34 and 35.

It should be understood that the term "SYR polypeptide or homologue thereof" should not be limited to the sequence represented by SEQ LD NO: 2 or to homologues listed as SEQ ID NO: 12 to SEQ ID NO: 25, but that any polypeptide of about from 65 to about 200 amino acids meeting the criteria of comprising a leucine rich domain as defined above, preceded by the conserved tripeptide motif 1 (a, b, c or d) and followed by the conserved motif 2 and preferably also the conserved motif 3 ; or having at least 38% sequence identity to the sequence of SEQ ID NO: 2, may be suitable for use in the methods of the invention.

In another embodiment, the present invention provides a SYR protein selected from the group consisting of:

(a) a polypeptide as given in SEQ ID NO 44,

(b) a polypeptide having an amino acid sequence having at least, in ascending order of preference, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94% 95%, 96%, 97%, 98% or 99% sequence identity to amino acid sequence as given in SEQ ID NO 44,

(c) a derivative of a protein as defined in (a) or (b).

The sequence represented by SEQ ID NO: 43 was hitherto unknown as a gene encoding SYR. Therefore an isolated nucleic acid sequence is provided comprising:

(i) a nucleic acid sequence represented by SEQ ID NO: 43, or the complementary ribbon thereof;

(ii) a nucleic acid sequence encoding the amino acid sequence represented by SEQ ID NO: 44;

(iii) a nucleic acid sequence capable of hybridizing (preferably under stringent conditions) to a nucleic acid sequence of (i) or (ii) above, whose hybridizing sequence preferably encodes a SYR protein; (iv) a nucleic acid which is an allelic variant for nucleic acid sequences according to (i) or (ii);

(v) a nucleic acid which is a splice variant for nucleic acid sequences according to (i) or (ii);

(vi) a nucleic acid sequence that has 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity with the sequence defined in (i) or (ii).

The activity of a SYR protein or homologue thereof can be assayed by expressing the SYR protein or homologue thereof under control of a GOS2 promoter in Oryza sativa, which results in increased seed yields without a delay in flowering time compared to plants. corresponding wild type. This increase in seed yield can be measured in a number of ways, for example as an increase in total seed weight, number of full grains or harvest index.

A SYR protein or homologue thereof is encoded by a nucleic acid / SYR gene. Therefore the term "nucleic acid / gene SYR" as defined herein is any nucleic acid / gene encoding a SYR protein or homologue thereof as defined above.

Examples of SYR nucleic acids include but are not limited to those represented by any of SEQ ID NO: 1, SEQ ID NO: 27 to SEQ ID NO: 32, SEQ ID NO: 36 to 42 and SEQ ID NO: 44. See also the list of nucleic acids mentioned in Table A of Example 1.

Nucleic acids / SYR genes and variants thereof may be suitable for practicing the methods of the invention. Variant SYR Nucleic Acids Genes include portions of a nucleic acid / SYR gene and / or nucleic acids capable of hybridizing to a nucleic acid / SYR gene.

The term portion as defined herein refers to a piece of DNA encoding a polypeptide of about 65 to about 200 amino acids, comprising a leucine-rich domain as defined above, preceded by the conserved tripeptide motif 1 (a, b, c or d) and followed by the conserved motif 2 and preferably also the preserved motif 3. Preferably, the portion comprises one or more of the conserved motifs defined above. A portion may be prepared, for example, by making one or more deletions for a SYR nucleic acid. The portions may be used in isolation or they may be fused to other coding (or non-coding) sequences to, for example, produce a protein that combines various activities. When fused to other coding sequences, the resulting polypeptide produced in translation may be larger than predicted for the SYR fragment. Preferably, the portion is a portion of a nucleic acid as represented by any one of SEQ ID NO: 1, SEQ ID NO: 27 to SEQ ID NO: 32, SEQ ID NO: 36 to SEQ ID NO: 42 and SEQ ID NO 44. More preferably the portion of a nucleic acid is as represented by SEQ ID NO: 1.

Another variant of a SYR nucleic acid / gene is a nucleic acid capable of hybridizing under reduced stringency conditions, preferably under stringent conditions, to a nucleic acid / SYR gene as defined above, whose hybridizing sequence encodes a polypeptide from about 65 to about of 200 amino acids, comprising a leucine rich domain as defined above, preceded by the conserved tripeptide motif 1 (a, b, c or d) and followed by the conserved motif 2 and preferably also by the conserved motif 3; or having at least 38% sequence identity with the sequence of SEQ ID NO: 2.

Preferably, the hybridizing sequence is one that is capable of hybridizing to a nucleic acid as represented by SEQ ID NO: 1, SEQ ID NO: 27 to SEQ ID NO: 32, SEQ ID NO: 36 to SEQ ID NO: 42 and SEQ ID NO: 44, or with a portion of any of the sequences mentioned above. More preferably the hybridizing sequence is capable of hybridizing to SEQ ID NO: 1. The term "hybridization" is as defined herein.

SYR nucleic acid or variant thereof may be derived from any natural or artificial source. The nucleic acid / gene or variant thereof may be isolated from a microbial source, such as yeast or fungi, or from a plant, algal or animal (including human) source. This nucleic acid may be modified from its native form in genomic composition and / or environment through deliberate human manipulation. The nucleic acid is preferably of plant origin, either from the same plant species (for example that into which it is introduced) or from a different plant species. The nucleic acid may be isolated from a monocot species, preferably from the Poaceae family, still preferably from Oryza sativa. More preferably, the nucleic acid SYR is isolated from Oryza sativa and is represented by SEQ ID NO: 1, and the amino acid sequence SYR is as represented by SEQID NO: 2.

Expression of a nucleic acid encoding a SYR polypeptide or homologue thereof can be modulated by introducing a genetic modification (preferably at the locus of a SYR gene). The locus of a gene as defined herein is adopted to mean a genomic region which includes the gene of interest and 10 kb before or after the coding region.

Genetic modification may be introduced, for example, by any (or more) of the following methods: T-DNA activation, TILLING, site-directed mutagenesis, transposon mutagenesis, direct evolution and homologous recombination, or by introducing and expressing in a plant a nucleic acid encoding a SYR polypeptide or homologue thereof. The methods mentioned above are defined here in the section titled "Definitions". Following introduction of genetic modification, there follows a step of selecting for modified expression of a nucleic acid encoding a SYR polypeptide or homologue thereof, whose modification in expression generates plants having increased seed production.

T-DNA5 TILLING activation, site-directed mutagenesis, transposon mutagenesis, and direct evolution are examples of technologies that enable the generation of new SYR alleles and variants.

A preferred method for introducing a genetic modification (which in this case need not be at the locus of a SYR gene) is to introduce and express into a plant a nucleic acid encoding a SYR polypeptide or homologue thereof, as defined herein. The nucleic acid to be introduced into a plant may be a full length nucleic acid or may be a hybridizing moiety or sequence as defined above.

"Homologs" of a protein are defined here in the section titled "Definitions". The SYR polypeptide or homologue thereof may be a derivative. For a definition of the term "derivative" see the section in this place titled "Definitions".

The SYR polypeptide or homologue thereof can be encoded by an alternative nucleic acid / SYR gene splice variant. The term "alternate union variant" is defined in the "Definitions" section. Preferred splice variants are nucleic acid splice variants encoding a polypeptide of about 65 to about 200 amino acids, comprising a leucine-rich domain as defined above, preceded by the conserved tripeptide motif 1 (a, b, c or d) and followed by the conserved motif 2 and preferably also by the conserved motif 3; or having at least 38% sequence identity with the sequence of SEQ ID NO: 2. Still preferred are union variants represented by SEQ ID NO: 1, SEQ ID NO: 27 to SEQ ID NO: 32, SEQ ID NO: 36 to SEQ ID NO: 42 and SEQ ID NO: 44. Most preferred is the splice variant represented by SEQ ID NO: 1. The homologue may also be encoded by an allelic variant of a nucleic acid encoding a SYR polypeptide or a homologous thereof, preferably an allelic variant of a nucleic acid encoding a polypeptide of about 65 to about 200 amino acids, comprising a leucine-rich domain as defined above, preceded by the conserved tripeptide motif 1 (a, b, c or d) and followed by conserved motif 2 and preferably also by conserved motif 3; or having at least 38% sequence identity to the sequence of SEQ ID NO: 2. Still preferably, the allelic variant encoding the SYR polypeptide is represented by any one of SEQ ID NO: 1, or SEQ ID NO: 12 to SEQ. More preferably, the allelic variant encoding the SYR polypeptide is as represented by SEQ ID NO: 1. The term "allelic variant" is defined in the "Definitions" section.

According to a preferred aspect of the present invention, increased expression of the SYR nucleic acid or variant thereof is contemplated. Methods for enhancing gene expression or gene products are well documented in the art and include, for example, overexpression directed by appropriate promoters, the use of transcriptional enhancers or translation enhancers. Isolated nucleic acids serving as promoter or enhancer elements may be introduced at an appropriate (typically before) position in a non-heterologous form of a polynucleotide in order to enhance expression of a SYR nucleic acid or variant thereof. For example, endogenous promoters may be altered in vivo by mutation, deletion, and / or substitution (see, Kmiec, U.S. Patent No. 5,565,350; Zarling et al., PCT / US93 / 03868), or isolated promoters. be introduced into a plant cell at the appropriate orientation and distance of a gene of the present invention to control gene expression. Methods for reducing gene expression or gene products are well documented in the art. If polypeptide expression is desired, it is generally desirable to include a polyadenylation region at the 3 'end of a polynucleotide coding region. The polyadenylation region may be derived from the natural gene, a variety of other plant genes, or T-DNA. The 3 'end sequence to be added may be derived, for example, from the nopaline synthase or octopine synthase genes, or alternatively from another plant gene, or less preferably from any other eukaryotic gene.

An intronic sequence can also be added to the untranslated 5 'region or coding sequence of the partial coding sequence to increase the amount of mature message that accumulates in the cytosol. Inclusion of a processable intron in the transcriptional unit in both plant and animal constructs has been shown to increase gene expression at both mRNA and protein levels by up to 1000-fold, Buchman and Berg, Mol. Cell biol. 8: 4395-4405 (1988); Callis et al., Genes Dev. 1: 1183-1200 (1987). Such intronic stimulation of gene expression is typically greater when placed near the 5 'end of the transcription unit. Using the introns of Adhl-S 1, 2, and 6 intron maize, the Bronze-I intron are known in the art. See generally, The Maize Handbook, Chapter 116, Freeling and Walbot, Eds., Springer, N.Y. (1994).

The invention also provides genetic constructs and vectors to facilitate introduction and / or expression of nucleotide sequences useful in the methods according to the invention.

Therefore, a gene construct is provided comprising:

(i) a nucleic acid SYR or variant thereof as defined above;

(ii) one or more control sequences capable of directing expression of the nucleic acid sequence of (i); and optionally

(iii) a transcription termination sequence; provided that the gene construct does not comprise a nucleic acid sequence encoding the protein of SEQ ID NO: 26.

Constructs useful in the methods according to the present invention may be constructed using recombinant DNA technology well known to those skilled in the art. Gene constructs may be inserted into commercially available vectors suitable for plant transformation and suitable for expression of the gene of interest in transformed cells.

Plants are transformed with a vector comprising the sequence of interest (i.e., a nucleic acid encoding a SYR polypeptide or homologue thereof). The sequence of interest is operably linked to one or more control sequences (at least one promoter). The terms "regulatory element", "control sequence" and "promoter" are all used interchangeably in this place and are defined here in the section titled "Definitions".

Advantageously, any type of promoter may be used to direct expression of the nucleic acid sequence. Preferably, the SYR nucleic acid or functional variant thereof is operably linked to a constitutive promoter. Preferably, the constitutive promoter capable of preferentially expressing nucleic acid throughout the plant has an expression profile comparable to a GOS2 promoter. More preferably, the constitutive promoter has the same expression profile as the rice GOS2 promoter, most preferably, the promoter capable of preferentially expressing nucleic acid throughout the plant is the rice GOS2 promoter (SEQ ID NO: 5).

It should be clear that the applicability of the present invention is not restricted to the SYR nucleic acid represented by SEQ ID NO: 1, nor is the applicability of the invention restricted to the expression of a SYR nucleic acid when directed by a GOS2 promoter. An alternative constitutive promoter that is useful in the methods of the present invention is the High Mobility Group Protein (HMGP) promoter (SEQ ID NO: 33). Examples of other constitutive promoters that may also be used to direct expression of a SYR nucleic acid are shown in Table 3 in the section entitled "Definitions".

Optionally, one or more terminator sequences may also be used in the construction introduced into a plant. The term "terminator" is defined in the "Definitions" section.

The genetic constructs of the invention may additionally include an origin of replication sequence that is required for maintenance and / or replication in a specific cell type. An example is when a genetic construct is required to be maintained in a bacterial cell as an episomal genetic element (e.g., plasmid or cosmid molecule). Preferred sources of replication include, but are not limited to, fl-ori and colEl.

The genetic construct may optionally comprise a selectable marker gene as defined in the "Definitions" section.

The present invention also encompasses plants obtainable by the methods according to the present invention. The present invention therefore provides plants obtainable by the method according to the present invention, which plants have introduced into them a nucleic acid SYR or variant thereof as defined above.

The invention also provides a method for producing transgenic plants having increased seed production comprising introducing and expressing in a plant a SYR nucleic acid or a variant thereof as defined above.

More specifically, the present invention provides a method for producing transgenic plants having increased seed production, which method comprises: (i) introducing and expressing into a plant or plant cell a SYR nucleic acid or variant thereof, and

(ii) cultivate the plant cell under conditions promoting plant growth and development;

provided that the nucleic acid SYR or variant thereof is not a nucleic acid sequence encoding the protein of SEQ ID NO: 26.

Nucleic acid can be introduced directly into a plant cell or into the plant itself (including introduction into a tissue, organ or any other part of a plant). According to a preferred feature of the present invention, the nucleic acid is preferably introduced into a plant by transformation. The term "transformation" is defined in the "Definitions" section.

The present invention clearly extends to any plant cell or plant produced by any of the methods described herein, and to all plant parts and seedlings thereof. The present invention further extends to encompass the progeny of a transformed or transfected primary cell, tissue, organ or whole plant that has been produced by any of the methods mentioned above, the sole requirement being that the progeny exhibit the same (s) genotypic and / or phenotypic trait (s) than those produced by the ancestor in the methods according to the invention. The invention also includes host cells containing an isolated or variant SYR nucleic acid. Preferred host cells according to the invention are plant cells. The invention also extends to harvestable parts of a plant such as, but not limited to seeds, leaves, fruits, flowers, stem crops, rhizomes, tubers and bulbs. The invention further relates to products directly derived from a harvestable part of such a plant, such as dried pellets or powders, oil, fat and fatty acids, starch or proteins.

The present invention also encompasses use of SYR nucleic acids or variants thereof and use of SYR polypeptides or homologues thereof.

One such use relates to improving plant growth characteristics, in particular to improve seed production. Seed production may include one or more of the following: increased total seed weight, increased number of full seeds, filling rate and increased harvest index.

SYR nucleic acids or variants thereof, or SYR polypeptides or homologues thereof may find use in breeding programs in which a DNA marker that can be genetically linked to a SYR gene or variant thereof is identified. SYR nucleic acids / genes or variants thereof, or SYR polypeptides or homologues thereof may be used to define a molecular marker. This DNA or protein marker can then be used in breeding programs to select plants having increased seed yield. The SYR gene or variant thereof, for example, may be a nucleic acid as represented by any one of SEQ ID NO: 1, SEQ ID NO: 27 to SEQ ID NO: 32, SEQ ID NO: 36 to SEQ ID NO: 42 and SEQ ID NO: 44.

Allelic variants of a nucleic acid / SYR gene may also find use in marker-assisted breeding programs. Such breeding programs sometimes require introduction of allelic variation by mutagenic treatment of plants, using for example EMS mutagenesis; alternatively, the program may start with a collection of allelic variants of the so-called "natural" origin caused unintentionally. Identification of allelic variants then occurs, for example, by PCR. This is followed by a step for selecting superior allelic variants of the sequence in question and generating increased seed yield. Selection is typically performed by monitoring plant growth performance containing different allelic variants of the sequence in question, for example, allelic variants different from any of SEQ ID NO: 1, SEQ ID NO: 27 to SEQ ID NO: 32, SEQ ID NO : 36 to SEQ ID NO: 42 and SEQ ID NO: 44. Growth performance can be monitored in a greenhouse or in the field. Additional optional steps include crossing plants in which the superior allelic variant has been identified with another plant. This can be used, for example, to make a combination of interesting phenotypic characteristics.

A SYR nucleic acid or variant thereof may also be used as probes to genetically and physically map the genes of which they are a part of, and as markers for traits linked to these genes. Such information may be useful in plant breeding in order to develop strains with desired phenotypes. Such use of SYR nucleic acids or variants thereof requires only a nucleic acid sequence of at least 15 nucleotides in length. SYR nucleic acids or variants thereof may be used as restriction fragment length polymorphism (RFLP) markers. Southern blots (Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning, A Laboratory Manual) of restriction digested plant genomic DNA can be probed with SYR nucleic acids or variants thereof. The resulting banding patterns can then be subjected to genetic analysis using computer programs such as MapMaker (Lander et al. (1987) Genomics 1: 174-181) in order to construct a genetic map. In addition, nucleic acids can be used to probe Southern blots containing restriction endonuclease-treated genomic DNAs from a set of individuals representing ancestor and progeny of a defined genetic crossover. Segregation of DNA polymorphisms is observed and used to calculate the position of SYR nucleic acid or variant on the genetic map previously obtained using this population (Botstein et al. (1980) Am. J. Hum. Genet. 32: 314-331) .

The production and use of plant gene derived probes for use in genetic mapping are described in Bernatzky and Tanksley (GENETICS 112 (4): 887-898, 1986). Numerous publications describe genetic mapping of specific cDNA clones using the methodology described above or variations thereof. For example, F2 crossover populations, inbreeding populations, randomly crossed populations, nearby isogenic strains, and other sets of individuals may be used for mapping. Such methodologies are well known to those skilled in the art.

Nucleic acid probes may also be used for physical mapping (ie, location of sequences in physical maps; see Hoheisel et al. In: Non-mammalian Genomic Analysis: A Practical Guide, Academic press 1996, pp. 319-346, and references cited there).

In another embodiment, nucleic acid probes may be used in direct mapping by fluorescence in situ hybridization (FISH) (Trask (1991) Trends Genet. 7: 149-154). Although current FISH mapping methods favor use of large clones (several kb to several hundred kb; see Laan et al. (1995) Genome Res. 5: 13-20), sensitivity improvements may allow FISH mapping performance using smaller probes.

A variety of nucleic acid amplification based methods for genetic and physical mapping can be performed using nucleic acids. Examples include allele-specific amplification (Kazazian (1989) J. Lab. Clin. Med. 11: 95-96), PCR amplified fragment polymorphism (CAPS; Sheffield et al. (1993) Genomics 16: 325-332), allele binding specific (Landegren et al. (1988) Science 241: 1077-1080), nucleotide extension reactions (Sokolov (1990) Nucleic Acid Res. 18: 3671), Radiation Hybrid Mapping (Walter et al. (1997) Nat Genet. 7: 22-28) and Appropriate Mapping (Dear and Cook (1989) Nucleic Acid Res. 17: 6795-6807). For these methods, the nucleic acid sequence is used to design and produce primer pairs for use in the amplification reaction or primer extension reactions. The design of such initiators is well known to those skilled in the art. In methods employing PCR-based genetic mapping, it may be necessary to identify DNA sequence differences between the mapping ancestors in the region corresponding to the immediate sequence of nucleic acids. This, however, is generally not required for mapping methods.

The methods according to the present invention result in plants having increased seed production as described above. These advantageous growth characteristics may also be combined with other economically advantageous characteristics, such as additionally features that stimulate production, tolerance to various stresses in addition to abiotic stress resistance, characteristics modifying various architectural attributes and / or biochemical and / or physiological attributes.

FG-GAP Detailed Description

The activity of a FG-GAP protein can be modulated by modulating levels of the FG-GAP polypeptide. Alternatively, activity can also be modulated when there are no changes in levels of an FG-GAP. This can occur when the intrinsic properties of the polypeptide are altered, for example by producing a mutant or by selecting a variant that is more active or less active than wild type.

The term "FG-GAP protein or homologue thereof" as defined herein refers to a polypeptide comprising (i) an N-terminal secretion signal peptide, (ii) one or more FG-GAP domains followed by (iii) a domain transmembrane in the C-terminal half of the protein. An example is given in Figure 6.

Signal peptides are typical for proteins that are directed to the secretory pathway. The presence of a secretion signal can easily be predicted using computational algorithms (eg SignalP 3.0, Bendtsen et al., J. Mol. Biol., 340: 783-795, 2004). A typical secretion signal consists of a positively charged η-region, followed by a hydrophobic n-region and a neutral polar c-region. In addition, amino acid residues at position -3 and -1 relative to the cleavage site are usually small and neutral.

Transmembrane domains are about 15 to 30 amino acids in length and are usually composed of hydrophobic residues that form an alpha helix. They are usually predicted on the basis of hydrophobicity (eg Klein et al., Biochim. Biophys. Acta 815, 468, 1985; or Sonnhammer et al., In J. Glasgow, T. Littlejohn, F. Major, R. Lathrop, D. Sankoff, and C. Sensen, editors, Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology, pages 175-182, Menlo Park, CA, 1998. AAAI Press.).

The FG-GAP domain (Pfam accession number PFO1839, INTERPRO entry IPR000413) is typically found in integrins where it is present as a repeat (up to 7 copies) in the extracellular part of the protein. So far, only animal integrins have been well characterized. The consensus sequence for the FG-GAP domain is given in SEQ ID NO: 53:

fgssvaagDlnGDGrpDlvvgaPgadggtdgsvyll, characterized in that the capital letters represent the single letter amino acid code for highly conserved amino acids and the other letters represent the single letter amino acid code for less conserved amino acids. The domain often comprises a Phe-Gly-Xn-Gly-Ala-Pro motif characterized by the fact that Xn represents a variable number of amino acids. Because this consensus sequence is derived from animal proteins, it is not fully compatible with plant FG-GAP domain sequences. For example, the hexapeptide "Pgadgg" may not be present in plant FG-GAP domains. Therefore, the term "FG-GAP domain" as used herein encompasses SEQ ID NO: 53 and sequences that have at least 40% sequence similarity to SEQ ID NO: 53, under alignment of SEQ ID NO: 53 and the sequence corresponding match, using the Needleman & Wunsch algorithm with a gap opening penalty of 10 with a gap length penalty of 0.5.

The FG-GAP domain may also comprise a Ca2 + binding site.

Preferably, the FG-GAP protein also comprises a

Motif 1 FDGYLYLI (D / E) G (SEQ ID NO: 50). More preferably, conserved motif 1 is FDGYLYLIDG.

Additionally and / or alternatively, the FG-GAP protein may comprise one or more DGXX (D / E) motifs (conserved motif 2, SEQ ID NO: 51), characterized in that X may be any amino acid. This conserved motif may be part of a larger DXDXDGXX (D / E) motif (conserved motif 3, SEQ ID NO: 52), characterized in that X can be any amino acid. Thus, the FG-GAP protein preferably comprises one or more copies of the conserved motif 3.

Alternatively, the homologue of an FG-GAP protein is in increasing order preferably 50%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65. %, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% , or 99% total sequence identity with the amino acid represented by SEQ ID NO: 46, provided that the homologous protein comprises a signal peptide sequence, one or more FG-GAP domains, and a transmembrane domain in the C-terminal half of preferably one or more of the conserved motifs 1, 2, or 3. The total sequence identity is determined using a global alignment algorithm, such as the Needleman Wunsch algorithm in the GAP (GCG Wisconsin Package, Accelrys) program, preferably with standard parameters and full length protein sequences.

The various structural domains in an FG-GAP protein can be identified using specialized databases eg SMART (Schultz et al (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864; Letunic et al (2002) Nucleic Acids Res 30, 242-244;), InterPro (Mulder et al., (2003) Nucl. Acids. Res. 31, 315-318;), Prosite (Bucher and Bairoch (1994), A generalized profile syntax for biomolecular sequences motifs and its funetion in automatic sequence interpretation. (In) ISMB-94; Proceedings 2nd International Conference on Intelligent Systems for Molecular Biology. Altman R., Brutlag D., Karp P., Lathrop R., Searls D., Eds. 53-61, AAAIPress, Menlo Park; Hulo et al., Nucl. Acids Res. 32: D134-D137 (2004)) or Pfam (Bateman et al., Nucleic Acids Research 30 (1): 276-280 (2002),).

Methods for searching and identifying FG-GAP counterparts would be well within the scope of those skilled in the art. Such methods comprise comparing sequences represented by SEQ ID NO: 45 or 46, in a computer readable format, with sequences that are available in public databases such as MIPS, GenBank or EMBL Nucleotide Sequence Database, using algorithms well known in the art for sequence alignment or comparison, such as GAP (Needleman and Wunsch, J. Mol. Biol. 48; 443-453 (1970)), BESTFIT (using the Smith and Waterman local homology algorithm ( Advances in Applied Mathematics 2; 482-489 (1981))), BLAST (Altschul, SF, Gish, W., Miller, W., Myers, EW & Lipman, DJ, J. Mol. Biol. 215: 403-410 (1990)), FASTA and TFASTA (WR Pearson and DJ Lipman Proc. Natl.Acad.Sci. USA 85: 2444-2448 (1988)). The computer application for performing BLAST analysis is publicly available through the National Center for Biotechnology Information (NCBI).

Examples of proteins falling under the definition of "FG-GAP polypeptide or a homologue thereof" include one Arabidopsis protein (SEQ ID NO: 55) and two rice proteins (SEQ ID NO: 57 and 59). The presence of FG-GAP proteins has also been demonstrated in other Magnoliophyta plant species, including Triticum aestivum, Zea mays, Solanum tuberosum, Aquilegia sp., Brassica napus, Citrus sinensis, Asparagus officinalis, Populus sp., Euphorbia esula. other plant taxa such as ferns (Ceratopteris richardii) or Welwitschia mirabilis. A non-limiting list of examples of ESTs encoding FG-GAP proteins is given in Table 8:

Table 8:

<table> table see original document page 76 </column> </row> <table>

Proteins encoded by the genes from which these ESTs are derived are also useful for practicing the methods of the present invention and fall within the scope of this invention. One skilled in the art may be able to isolate the full length coding sequence of these genes using standard methods.

The invention further provides an isolated FG-GAP protein selected from the group consisting of:

(a) a nucleic acid encoded protein of SEQ ID NO: 72;

(b) a protein comprising a signal sequence, one or more FG-GAP domains and a transmembrane domain located at the C-terminal half of the protein, characterized in that said protein comprises at least one of SEQ ID NO: 73 to SEQ ID NO: 72;

(c) an active fragment of an amino acid sequence as defined in (a) or (b), whose active fragment comprises a signal sequence, one or more FG-GAP domains, and a transmembrane domain located at the C-terminal half of the protein.

It should be understood that the term "FG-GAP polypeptide or a homologue thereof" should not be limited to the sequence represented by SEQ ID NO: 46 or the homologues listed as SEQ ID NO: 55, 57 and 59, but that any polypeptide assembling the criteria comprising a signal peptide, one or more FG-GAP domains and a transmembrane domain located at the C-terminal half of the protein, and preferably also one or more of the conserved motifs of SEQ ID NO: 50 to 52; or having at least 50% sequence identity with the sequence of SEQ ID NO: 46, may be suitable for use in the methods of the invention.

Plant FG-GAP proteins play a role during pollen development (Paxson-Sowders et al. 2001). In dexl mutant plants, primexin deposition is delayed and significantly reduced. Normal plasma membrane ripple and spacer production observed in wild-type plants is also absent in the mutant. FG-GAP proteins are able to complement this mutation and restore the normal phenotype.

Alternatively, the activity of an FG-GAP protein or homologue thereof may be assayed by expressing the FG-GAP protein or homologue thereof under control of a constitutive promoter in Oryza sativa, which results in plants with increased above ground biomass and / or yield. compared to corresponding wild type plants. This increase in seed yield can be measured in a number of ways, for example as an increase in total seed weight, number of full grains or total number of seeds.

An FG-GAP protein or homologue thereof is encoded by a FG-GAP nucleic acid / gene. Therefore the term "FG-GAP nucleic acid / gene" as defined herein is any nucleic acid / gene encoding an FG-GAP protein or homologue thereof as defined above.

Examples of FG-GAP nucleic acids include but are not limited to those represented by any of SEQ ID NO: 45, SEQ ID NO: 54, SEQ ID NO: 56 or SEQ ID NO: 58. Examples of partial FG-GAP nucleic acids are listed in Table 8.

The invention also provides an isolated nucleic acid encoding an FG-GAP protein selected from the group consisting of:

(i) nucleic acid as represented in SEQ ID NO: 72;

(ii) a nucleic acid encoding a protein as defined in (a) to (c) above;

(iii) a nucleic acid sequence capable of hybridizing (preferably under stringent conditions) to a nucleic acid sequence of (i) or (ii) above, whose hybridizing sequence preferably encodes a protein comprising a signal peptide, one or more FG domains -GAP is a transmembrane domain located at the C-terminal half of the protein;

(iv) a nucleic acid which is an allelic variant for nucleic acid sequences according to (i) to (iii);

(v) a nucleic acid which is an alternative splice variant for nucleic acid sequences according to (i) to (iii);

(vi) a portion of a nucleic acid sequence according to any of (i) to (v) above, which portion preferably encodes a protein comprising a signal peptide, one or more FG-GAP domains, and a half-transmembrane domain C-terminal protein.

FG-GAP nucleic acids / genes and variants thereof may be suitable for practicing the methods of the invention. Variant FG-GAP nucleic acids / genes include portions of a FG-GAP nucleic acid / gene, allelic variants, splice variants and / or nucleic acids capable of hybridizing to a FG-GAP nucleic acid / gene.

The term portion as defined herein refers to a piece of DNA encoding a polypeptide comprising a signal peptide, one or more FG-GAP domains and a transmembrane domain located at the C-terminal half of the protein, and preferably also one or more of the motifs. SEQ ID NO: 50 to 52. Preferably, the portion comprises one or more of the conserved motifs defined above. A portion may be prepared, for example, by making one or more deletions to an FG-GAP nucleic acid. The portions may be used in isolation or they may be fused to other coding (or non-coding) sequences to, for example, produce a protein that combines various activities. When fused to other coding sequences, the resulting translation produced polypeptide may be larger than predicted for the FG-GAP fragment. Preferably, the portion is a portion of a nucleic acid as represented by any of SEQ ID NO: 45, SEQ ID NO: 54, SEQ ID NO: 56, SEQ ID NO: 58 or SEQ ID NO: 72. The portion also can be a portion of the coding sequences from which the sequences in Table 8 are derived. More preferably the portion of a nucleic acid is as represented by SEQ ID NO: 45.

Another variant of a FG-GAP nucleic acid / gene is a nucleic acid capable of hybridizing under reduced stringency conditions, preferably under stringent conditions, to a FG-GAP nucleic acid / gene as defined above, whose hybridizing sequence encodes a polypeptide comprising a signal peptide, one or more FG-GAP domains and a transmembrane domain located at the C-terminal half of the protein, and preferably also one or more of the conserved motifs of SEQ ID NO: 50 to 52.

Preferably, the hybridizing sequence is one that is capable of hybridizing to a nucleic acid as represented by SEQ ID NO: 45, SEQ ID NO: 54, SEQ ID NO: 56, SEQ ID NO: 58, or SEQ ID NO: 72, or with a portion of any of the sequences mentioned above, including the ESTs listed in Table 8. More preferably the hybridizing sequence is capable of hybridizing to SEQ ID NO: 45. The term "hybridization" is as defined in the section entitled "Definitions".

FG-GAP nucleic acid or variant thereof may be derived from any natural or artificial source. The nucleic acid / gene or variant thereof may be isolated from a microbial source, such as yeast or fungi, or from a plant, algal or animal (including human) source. This nucleic acid may be modified from its native form in genomic composition and / or environment through deliberate human manipulation. The nucleic acid is preferably of plant origin, either from the same plant species (for example that into which it is introduced) or from a different plant species. The nucleic acid may be isolated from a dicotyledonous species, preferably from the Brassicaceae family, still preferably from Arabidopsis thaliana. More preferably, the FG-GAP nucleic acid is isolated from Arabidopsis thaliana and is represented by SEQ ID NO: 45, and the FG-GAP amino acid sequence is as represented by SEQ ID NO: 46. The expression of a nucleic acid encoding a FG-GAP polypeptide or a homologue thereof can be modulated by introducing a genetic modification (preferably at the locus of an FG-GAP gene). The locus of a gene as defined herein is adopted to mean a genomic region which includes the gene of interest and 10 kb before or after the coding region.

Genetic modification may be introduced, for example, by any (or more) of the following methods: T-DNA activation, TILLING, site-directed mutagenesis, transposon mutagenesis, direct evolution and homologous recombination, or by introducing and expressing in a plant a nucleic acid encoding an FG-GAP polypeptide or homologue thereof. These methods are defined in the section titled "Definitions". Following introduction of genetic modification, there follows a step of selecting for modified expression of a nucleic acid encoding an FG-GAP polypeptide or homologue thereof, whose modification in expression generates plants having increased yield.

T-DNA activation, TILLING, site-directed mutagenesis, transposon mutagenesis, and direct evolution are examples of technologies that enable the generation of new FG-GAP alleles and variants.

A preferred method for introducing a genetic modification (which in this case need not be at the locus of an FG-GAP gene) is to introduce and express into a plant a nucleic acid encoding an FG-GAP polypeptide or homologue thereof as defined above. The nucleic acid to be introduced into a plant may be a full length nucleic acid or may be a hybridizing moiety or sequence as defined above. Preferably, the plant into which the genetic modification is introduced is not a dexl mutant plant in which the DEX1 gene is not functional (Paxson-Sowders et al. 2001).

"Homologs" of a protein are defined in the section entitled "Definitions". The FG-GAP polypeptide or homologue thereof may be a derivative as defined in the "Definitions" section.

The FG-GAP polypeptide or homologue thereof can be encoded by an alternative nucleic acid / FG-GAP gene splice variant. The term "alternative joining variant" is as defined herein. Nucleic acid binding variants encoding a polypeptide comprising a signal peptide, one or more FG-GAP domains and a transmembrane domain located at the C-terminal half of the protein, and preferably also one or more of the conserved motifs of SEQ ID NO: are preferred. 50 to 52. Still preferred are binding variants represented by SEQ ID NO: 45, SEQ ID NO: 54, SEQ ID NO: 56 or SEQ ID NO: 58, or a nucleic acid binding variant represented by SEQ ID NO: 72, or a splice variant of one of the genes from which the sequences in Table 8 are derived. Most preferred is the joint variant represented by SEQ ID NO: 45.

The homologue may also be encoded by an allelic variant of a nucleic acid encoding an FG-GAP polypeptide or a homologue thereof, preferably an allelic variant of a nucleic acid encoding a polypeptide comprising a signal peptide, one or more FG-GAP domains and a transmembrane domain located at the C-terminal half of the protein, and preferably also one or more of the conserved motifs of SEQ ID NO: 50 to 52. Still preferably, the allelic variant encoding the FG-GAP polypeptide is represented by any one of SEQ ID NO. : 45, SEQ ID NO: 54, SEQ ID NO: 56 or SEQ ID NO: 58. More preferably, the allelic variant encoding the FG-GAP polypeptide is as represented by SEQ ID NO: 45. Allelic variants are defined in the " Definitions".

In a preferred aspect of the present invention, modulated expression of the FG-GAP nucleic acid or variant thereof is contemplated. Preferably, the modulated expression is overexpression. Methods for overexpression of genes or gene products are well documented in the art and include, for example, overexpression directed by appropriate promoters, the use of transcription enhancers or translation enhancers. Isolated nucleic acids serving as promoter or enhancer elements may be introduced at an appropriate (typically before) position in a nonheterologous form of a polynucleotide in order to enhance expression of a FG-GAP nucleic acid or variant thereof. For example, endogenous promoters may be altered in vivo by mutation, deletion, and / or substitution (see, Kmiec, U.S. Patent No. 5,565,350; Zarling et al., PCT / US93 / 03868), or isolated promoters. be introduced into a plant cell at the appropriate orientation and distance of a gene of the present invention to control gene expression. Methods for reducing gene expression or gene products are also well documented in the art.

If polypeptide expression is desired, it is generally desirable to include a polyadenylation region at the 3 'end of a polynucleotide coding region. The polyadenylation region may be derived from the natural gene, a variety of other plant genes, or T-DNA. The 3 'end sequence to be added may be derived, for example, from the nopaline synthase or octopine synthase genes, or alternatively from another plant gene, or less preferably from any other eukaryotic gene.

An intronic sequence can also be added to the untranslated 5 'region or coding sequence of the partial coding sequence to increase the amount of mature message that accumulates in the cytosol. Inclusion of a processable intron in the transcriptional unit in both plant and animal constructs has been shown to increase gene expression at both mRNA and protein levels by up to 1000-fold, Buchman and Berg, Mol. Cell biol. 8: 4395-4405 (1988); Callis et al., Genes Dev. 1: 1183-1200 (1987). Such intronic stimulation of gene expression is typically greater when located near the 5 'end of the transcription unit. Using the introns of Adh1-S 1, 2, and 6 intron maize, the Bronze-1 intron are known in the art. See generally, The Maize Handbook, Chapter 116, Freeling and Walbot, Eds., Springer, Ν. Υ (1994).

The invention also provides genetic constructs and vectors to facilitate introduction and / or expression of nucleotide sequences useful in the methods according to the invention.

Therefore, a gene construct is provided comprising:

(i) an FG-GAP nucleic acid or variant thereof as defined above;

(ii) one or more control sequences capable of directing expression of the nucleic acid sequence of (i); and optionally

(iii) a transcription termination sequence; provided that the gene construct is not a pPZP-type gene construct as described by Hajdukiewicz et al. (Plant Mol. BioL 25, 989-994) and Paxson-Sowders (2001).

Constructs useful in the methods according to the present invention may be constructed using recombinant DNA technology well known to those skilled in the art. Gene constructs may be inserted into commercially available vectors suitable for plant transformation and suitable for expression of the gene of interest in transformed cells.

Plants are transformed with a vector comprising the sequence of interest (i.e., a nucleic acid encoding an FG-GAP polypeptide or homologue thereof). The sequence of interest is operably linked to one or more control sequences (at least one promoter). The terms "regulatory element", "control sequence" and "promoter" are all used interchangeably in this place and are defined in the section entitled "Definitions".

Advantageously, any type of promoter may be used to direct expression of the nucleic acid sequence. Preferably, the FG-GAP nucleic acid or functional variant thereof is operably linked to a constitutive promoter. The term "constitutive" is as defined herein. Preferably, the constitutive promoter capable of preferentially expressing nucleic acid throughout the plant has an expression profile comparable to a GOS2 promoter. More preferably, the constitutive promoter has the same expression profile as the rice GOS2 promoter, most preferably, the promoter capable of preferentially expressing the entire plant nucleic acid is the rice GOS2 promoter (nucleotides 1 to 2193 of the sequence represented in SEQ ID NO: 49). It should be clear that the applicability of the present invention is not restricted to the FG-GAP nucleic acid represented by SEQ ID NO: 45, nor is the applicability of the invention restricted to the expression of an FG-GAP nucleic acid when directed by a GOS2 promoter. Examples of other constitutive promoters that may also be used to direct expression of an FG-GAP nucleic acid are shown in Table 3 in the "Definitions" section.

Optionally, one or more terminator sequences may also be used in the construction introduced into a plant. The term "terminator" being defined in the "Definitions" section.

The genetic constructs of the invention may additionally include an origin of replication sequence that is required for maintenance and / or replication in a specific cell type. An example is when a genetic construct is required to be maintained in a bacterial cell as an episomal genetic element (e.g., plasmid or cosmid molecule). Preferred sources of replication include, but are not limited to, fl-ori and colEl.

The genetic construct may optionally comprise a selectable marker gene as defined in the "Definitions" section herein.

The present invention also encompasses plants obtainable by the methods according to the present invention. The present invention therefore provides plants obtainable by the method according to the present invention, which plants have introduced into them an FG-GAP nucleic acid or variant thereof as defined above.

The invention also provides a method for producing transgenic plants having increased production comprising introducing and expressing in a plant an FG-GAP nucleic acid or a variant thereof as defined above.

More specifically, the present invention provides the method for producing transgenic plants having increased yield, which method comprises:

(i) introducing and expressing into the plant or plant cell an FG-GAP nucleic acid or variant thereof; and

(ii) cultivate the plant cell under conditions promoting plant growth and development.

Nucleic acid can be introduced directly into a plant cell or into the plant itself (including introduction into a tissue, organ or any other part of a plant). According to a preferred feature of the present invention, the nucleic acid is preferably introduced into a plant by transformation.

The term "transformation" is as defined in the "Definitions" section.

The present invention clearly extends to any plant cell or plant produced by any of the methods described herein, and to all plant parts and seedlings thereof. The present invention further extends to encompass the progeny of a transformed or transfected primary cell, tissue, organ or whole plant that has been produced by any of the methods mentioned above, the sole requirement being that the progeny exhibit the same (s) genotypic and / or phenotypic trait (s) than those produced by the ancestor in the methods according to the invention. The invention also includes host cells containing an isolated or variant FG-GAP nucleic acid. Preferred host cells according to the invention are plant cells. The invention also extends to harvestable parts of a plant such as, but not limited to seeds, leaves, fruits, flowers, stem crops, rhizomes, tubers and bulbs. The invention furthermore relates to products derived from, preferably directly derived from, a harvestable part of such a plant, such as dried pellets or powders, oil, fat and fatty acids, starch and proteins.

The present invention also encompasses use of FG-GAP nucleic acids or variants thereof and use of FG-GAP polypeptides or homologues thereof.

Such use refers to improving plant growth characteristics, in particular to improve yields, especially seed yields. Seed production may include one or more of the following: increased total seed weight, increased number of full seeds and increased total number of seeds.

FG-GAP nucleic acids or variants thereof, or FG-GAP polypeptides or homologues thereof may find use in breeding programs in which a DNA marker that can be genetically linked to an FG-GAP gene or variant thereof is identified. FG-GAP nucleic acids / genes or variants thereof, or FG-GAP polypeptides or homologues thereof may be used to define a molecular marker. This DNA or protein marker can then be used in breeding programs to select plants having increased yield. The FG-GAP gene or variant thereof, for example, may be a nucleic acid as represented by any of SEQ ID NO: 45, SEQ ID NO: 54, SEQ ID NO: 56, SEQ ID NO: 58, and SEQ ID NO: 72, or genes from which the sequences listed in Table 8 were derived.

Allelic variants of a FG-GAP nucleic acid / gene may also find use in marker-assisted breeding programs. Such breeding programs sometimes require introduction of allelic variation by mutagenic treatment of plants, using for example EMS mutagenesis; alternatively, the program may start with a collection of allelic variants of the so-called "natural" origin caused unintentionally. Identification of allelic variants then occurs, for example, by PCR. This is followed by a step for selecting superior allelic variants of the sequence in question and generating increased production. Selection is typically performed by monitoring growth performance of plants containing different allelic variants of the sequence in question, for example, allelic variants different from any of SEQ ID NO: 45, SEQ ID NO: 54, SEQ ID NO: 56, SEQ ID NO : 58, and SEQ ID NO: 72, or one of the coding sequences from which the sequences listed in Table 8 were derived. Growth performance can be monitored in a greenhouse or in the field. Additional optional steps include crossing plants in which the superior allelic variant has been identified with another plant. This can be used, for example, to make a combination of interesting phenotypic characteristics.

An FG-GAP nucleic acid or variant thereof can also be used as probes to genetically and physically map the genes of which they are a part of, and as markers for traits linked to these genes. Such information may be useful in plant breeding in order to develop strains with desired phenotypes. Such use of FG-GAP nucleic acids or variants thereof requires only a nucleic acid sequence of at least 15 nucleotides in length. FG-GAP nucleic acids or variants thereof may be used as restriction fragment length polymorphism (RFLP) markers. Southern blots (Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning, A Laboratory Manual) of restriction digested plant genomic DNA can be probed with FG-GAP nucleic acids or variants thereof. The resulting banding patterns can then be subjected to genetic analysis using computer programs such as MapMaker (Lander et al. (1987) Genomics 1: 174-181) in order to construct a genetic map. In addition, nucleic acids can be used to probe Southern blots containing restriction endonuclease-treated genomic DNAs from a set of individuals representing ancestor and progeny of a defined genetic crossover. Segregation of DNA polymorphisms is observed and used to calculate the position of FG-GAP nucleic acid or variant on the genetic map previously obtained using this population (Botstein et al. (1980) Am. J. Hum. Genet. 32: 314- 331).

The production and use of plant gene derived probes for use in genetic mapping are described in Bernatzky and Tanksley (Plant Mol. Biol. Reporter 4: 37-41, 1986). Numerous publications describe genetic mapping of specific cDNA clones using the methodology described above or variations thereof. For example, F2 crossover populations, inbreeding populations, randomly crossed populations, nearby isogenic strains, and other sets of individuals may be used for mapping. Such methodologies are well known to those skilled in the art.

Nucleic acid probes may also be used for physical mapping (ie, location of sequences in physical maps; see Hoheisel et al. In: Non-mammalian Genomic Analysis: A Practical Guide, Academic press 1996, pp. 319-346, and references cited there).

In another embodiment, nucleic acid probes may be used in direct mapping by fluorescence in situ hybridization (FISH) (Trask (1991) Trends Genet. 7: 149-154). Although current FISH mapping methods favor the use of large clones (several kb to several hundred kb; see Laan et al. (1995) Genome Res. 5: 13-20), sensitivity improvements may allow FISH mapping performance using probes. smaller ones.

A variety of nucleic acid amplification based methods for genetic and physical mapping can be performed using nucleic acids. Examples include allele-specific amplification (Kazazian (1989) J. Lab. Clin. Med. 11: 95-96), PCR amplified fragment polymorphism (CAPS; Sheffield et al. (1993) Genomics 16: 325-332), allele binding specific (Landegren et al. (1988) Science 241: 1077-1080), nucleotide extension reactions (Sokolov (1990) Nucleic Acid Res. 18: 3671), Radiation Hybrid Mapping (Walter et al. (1997) Nat Genet. 7: 22-28) and Appropriate Mapping (Dear and Cook (1989) Nucleic Acid Res. 17: 6795-6807). For these methods, the nucleic acid sequence is used to design and produce primer pairs for use in the amplification reaction or primer extension reactions. The design of such initiators is well known to those skilled in the art. In methods employing PCR-based genetic mapping, it may be necessary to identify DNA sequence differences between the mapping ancestors in the region corresponding to the immediate sequence of nucleic acids. This, however, is generally not required for mapping methods.

The methods according to the present invention result in plants having increased yield as described above. These advantageous growth characteristics may also be combined with other economically advantageous characteristics, such as additional characteristics that stimulate production, tolerance to various stresses, characteristics modifying various architectural attributes and / or biochemical and / or physiological attributes.

CYP90B Detailed Description

The term "CYP90B polypeptide or homologue thereof" as defined herein refers to a polypeptide comprising the following: (a) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position.

In addition, the CYP90B polypeptide or homologue thereof may further comprise (i) a sequence with more than 50% identity to SEQ ID NO: 78 and (ii) steroid 22 alpha hydroxylase enzymatic activity.

Examples of a CYP90B polypeptide as defined above are given in Table 9a herein.

A CYP90B polypeptide or homologue thereof is encoded by a CYP90B nucleic acid / gene. Therefore the term "CYP90B nucleic acid / gene" as defined herein is any nucleic acid / gene encoding a CYP90B polypeptide or homologue thereof as defined above.

The various structural domains found in the CYP superfamily of proteins, including CYP90B polypeptides of the present invention, are well known in the art and can be identified using general databases eg SMART (Schultz et al. (1998) Proc. Natl. Acad. Sci USA 95, 5857-5864; Letunic et al. (2002) Nucleic Acids Res 30, 242-244; http://smart.embl-heidelberg.de/), InterPro (Mulder et al. (2003) Nucl. Acids 31, 315-318; http://www.ebi.ac.uk/interpro/), Prosite (Bucher and Bairoch (1994), A generalized profile syntax for biomolecular sequence motifs and its funetion in automatic sequence interpretation, in ISMB-94; Proceedings 2nd International Conference on Intelligent Systems for Molecular Biology Altman R., Brutlag D., Karp P., Lathrop R., Searls D., Eds., pp53-61, AAAI Press, Menlo Park; Hulo et al., Nucl. Acids Res. 32.-D134-D137, (2004), http://www.expasy.org/prosite/) or Pfam (Bateman et al., Nucleic Acids Research 30 (l): 276 -280 (2002), http: // www.sanger.ac.uk/Sofitware/Pfam/}.

Specialized databases can also be searched at http://arabidopsis-P450.biotec.uiuc.edu/cgi-bin/p450.pl for Arabidopsis, or more generally on the CYP Website http://drnelson.utmem. edu / CytochromeP450.html. Typical structural domains found in CYP may be the four AaD domains as originally described by Kalb & Loper ((1988) Proc Natl Acad Sci 85: 7221-7225). Domain A (also called Helix I) comprises the consensus sequence Ala / Gly-Gly-X-Asp / Glu-Thr-Thr / Ser, and is proposed to bind to dioxigen. Domain B is the steroid binding domain. The D domain corresponds to the heme binding domain and comprises the most characteristic CYP amino acid consensus sequence (Phe-X-X-Gly-X-Arg-X-Cys-X-Gly) (Figures 10 and 13).

The presence of consensus sequences can be identified using methods for sequence alignment for comparison as described above. In some instances, the default parameters may be adjusted to modify the search stringency. For example using BLAST, the threshold of statistical significance (called the "expected" value) for reporting identities against database sequences can be increased to show less stringent identities. In this way, practically exact short identities can be identified. The Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence within domain A of the CYP90B polypeptide (comprising the Ala / Gly-Gly-X-Asp / Glu- Thr-Thr / Ser consensus sequence as defined above) ) as defined herein can be identified in this manner, as a person skilled in the art should be well aware.

Another domain identified in CYP P450 proteins, and in particular the CYP90B polypeptide of the invention, may be the N-terminus anchor domain of the membrane-targeting protein rich in hydrophobic residues such as Leu, Ile, Val, pH and Ala. The N-terminal anchor domain is typically between 20 and 40 amino acids in length, but may be smaller (up to 10 amino acids) or larger (up to 100 amino acids). The N-terminal anchor domain is separated from the rest of the protein (globular domain) by a transition domain comprising a grouping of basic residues (at least two, or Lys or Arg, called the transfer interrupt signal) preceding a proline grouping. forming a hinge between the anchor domain mentioned above and the globular domain of the protein. A typical consensus sequence for the transition domain is Lys / Arg-Lys / Arg- (X) 3-9-Pro-Pro-Gly (Figures 10 and 13). Such a consensus sequence can be identified as mentioned above.

The presence of an N-terminal hydrophobic anchor domain can be readily identified. Primary amino acid composition (in%) to determine if a polypeptide domain is rich in specific amino acids can be calculated using ExPASy server computer application programs, in particular the ProtParam tool (Gasteiger E et al. (2003) ExPASy: the server of proteomics for knowledge and detailed protein analysis (Nucleic Acids Res 31: 3784-3788). The protein composition of interest can then be compared to the average amino acid composition (in%) in the Swiss-Prot Protein Sequence database. Within this database, the mean addition of Leu (L), Ile (I), Val (V), pH e (F) and Ala (A) is 34.04%. As an example, the N-terminal hydrophobic anchor domain of SEQ ID NO: 78 contains 62.5% of the same hydrophobic residues. As defined herein, an N-terminal hydrophobic anchor domain has a hydrophobic amino acid content (in terms of%) higher than that in the average amino acid composition (in terms of%) of proteins in the Swiss-Prot Protein Sequence database. .

Special computational applications such as ProtScale (Gasteiger et al. (2005) Protein Identification and Analysis Tools on the ExPASy Server. In John M. Walker, ed: The Proteomics Protocols Handbook, Humana Press pp. 571-607) compute and represent the profile. produced by any scale of amino acids in a selected protein. An amino acid scale is defined by a numerical value assigned to each type of amino acid. The most frequently used scales are hydrophobicity or hydrophilicity scales and secondary structure conformational parameter scales. One of the most frequently used amino acid hydrophobicity scales was produced by Kyte & Doolittle ((1982) J. Mol. Biol. 157: 105-132), in which hydrophobic amino acids were assigned a positive number, and hydrophilic amino acids a negative number.

For example, the ProtScale result profile for hydrophobicity of the CYP90B polypeptide of the invention clearly shows that approximately the first 34 N-terminal amino acids represent a hydrophobic domain as they are located above the zero delimiting line (Figure 12). This region corresponds to the N-terminal anchor domain. A person skilled in the art should be well aware of such analyzes.

CYP90B polypeptides or homologues thereof can be readily identified using routine techniques well known in the art, such as by sequence alignment. Methods for sequence alignment for comparison are well known in the art, such methods include GAP, BESTFIT, BLAST, FASTA and TFASTA. GAP uses the algorithm of Needleman and Wunsch ((1970) J Mol Biol 48: 443-453) to find the alignment of two complete sequences that maximizes the number of identities and minimizes the number of gaps. The BLAST algorithm (Altschul et al. (1990) J Mol Biol 215: 403-10) calculates percent sequence identity and performs a statistical analysis of similarity between the two sequences. The computer application for performing BLAST analysis is publicly available through the National Center for Biotechnology Information. CYP90B homologues comprising a sequence with more than 50% identity with SEQ ID NO: 78 can be readily identified using, for example, the ClustalW multiple sequence alignment algorithm (version 1.83) available at http://clustalw.genome .jp / sit-bin / nph-ClustalW, with the standard paired alignment parameters, and a percentage scoring method. Secondary manual editing can be performed to optimize alignment between retained subjects, as should be apparent to a person skilled in the art.

Examples of or homologous CYP90B polypeptides (encoded by accession number polynucleotide sequence in parentheses) are given in Table 9a. Table 9b supports partial CYP90B sequences encoding partial CYP90B open reading frames (ORF).

Table 9: a) Examples of CYP90B homologues

<table> table see original document page 95 </column> </row> <table> Table 9: b) Examples of CYP90B with a partial open reading frame (ORF)

<table> table see original document page 96 </column> </row> <table>

* Manual processing from genomic clone

** Sequence grouping compiled from several EST accesses (the main ones shown); If EST sequencing quality is usually inferior, a few amino acid substitutions can be expected.

It should be understood that sequences falling under the definition of "CYP90B polypeptide or homologue thereof" should not be limited to the sequences represented by SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88 or SEQ ID NO: 90, but any polypeptide comprising the following: (a) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position may be suitable for use in performance of the invention.

Sequences falling under the definition of "CYP90B polypeptide or homologue thereof" may additionally comprise (i) a sequence with more than 50% identity to SEQ ID NO: 78 and (ii) steroid 22 alpha hydroxylase enzymatic activity.

CYP90B polypeptides or homologues thereof have 22-alpha hydroxylase enzymatic activity, which can be determined by complementation testing using plants having a DWF4 mutation. Such mutant plants are described in Arabidopsis (dw / 4 mutant) by Choe et al. ((1998) Plant Cell 10: 231-243) and in rice (Tos2091 mutant) by Tanaka et al (US2004 / 0060079). The size of these mutant plants is several times smaller than that of their corresponding wild type, i.e., the mutant plants are overgrown. The isolated polypeptide is placed under the control of a promoter capable of expressing this polypeptide in plants in a recombinant DNA vector suitable for plant transformation. The mutant plants are then transformed with this vector using techniques that are well known in the art. If the transformed plants no longer have the over-wasted phenotype, it is indicative that the isolated polypeptide is capable of enzymatic 22-alpha hydroxylase activity. Such a polypeptide may be suitable for use in performing the methods of the invention.

Examples of CYP90B nucleic acids include but are not limited to those represented by any of SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87 or SEQ ID NO: 89. Nucleic acids / CYP90B genes and variants thereof may be suitable for practicing the methods of the invention. Nucleic acid / CYP90B gene variants include portions of a CYP90B nucleic acid / gene and / or nucleic acids capable of hybridizing to a CYP90B nucleic acid / gene. The term portion as defined herein refers to a piece of DNA encoding a polypeptide comprising the following: (a) CYP P450 AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position. A portion may be prepared, for example, by making one or more deletions to a CYP90B nucleic acid. The portions may be used in isolation or they may be fused to other coding (or non-coding) sequences to, for example, produce a protein that combines various activities. When fused to other coding sequences, the resulting translation produced polypeptide may be larger than that predicted for the CYP90B moiety. Preferably, the portion is a portion of a nucleic acid as represented by any of SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO. : 87 and SEQ ID NO: 89. More preferably the portion is a portion of a nucleic acid as represented by SEQ ID NO: 77.

Another variant of a CYP90B nucleic acid / gene is a nucleic acid capable of hybridizing under reduced stringency conditions, preferably under stringent conditions, to a CYP90B nucleic acid / gene as defined above, whose hybridizing sequence encodes a polypeptide comprising the following: ) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, allowing an amino acid change at any position. Preferably, the hybridizing sequence is one that is capable of hybridizing to a nucleic acid as represented by any of SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87 and SEQ ID NO: 89, or with a portion of any of the sequences mentioned above as defined above. More preferably the hybridizing sequence is one which is capable of hybridizing to a nucleic acid as represented by SEQ LD NO: 77. The term "hybridization" is as defined herein in the "Definitions" section.

CYP90B nucleic acid or variant thereof may be derived from any natural or artificial source. The nucleic acid / gene or variant thereof may be isolated from a microbial source, such as yeast or fungi, or from a plant, algal or animal (including human) source. This nucleic acid may be modified from its native form in genomic composition and / or environment through deliberate human manipulation. The nucleic acid is preferably of plant origin, either from the same plant species (for example that into which it is introduced) or from a different plant species. The nucleic acid may be isolated from a monocot species, preferably from the Poaceae family, still preferably from the genus Oryza, most preferably from Oryza sativa. More preferably, the CYP90B nucleic acid isolated from Oryza sativa is represented by SEQ ID NO: 77 and the amino acid sequence CYP90B is as represented by SEQ ID NO: 78.

The invention further provides an isolated CYP90B protein selected from the group consisting of:

(a) a protein encoded by the nucleic acid of SEQ ID NO: 117;

(b) a protein comprising the following: (i) CYP AaD domains; (ii) an N-terminal hydrophobic anchor domain; (iii) a transition domain; and (iv) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position, and in ascending order of preference at least 85%. %, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% identity with the amino acid sequence of SEQ ID NO: 118. The invention also provides an isolated nucleic acid encoding a CYP90B protein selected from the group consisting of:

(i) a nucleic acid as represented by SEQ ID NO: 117;

(ii) a nucleic acid encoding a protein as defined in (a) and (b) above;

(iii) a nucleic acid having in ascending order preferably at least 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more nucleic acid identity represented by SEQ ID NO: 117;

(iv) a nucleic acid sequence capable of hybridizing under stringent conditions to a nucleic acid sequence of (i) to (iii) above, whose hybridizing sequence encodes a protein comprising (a) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position, and preferably in increasing order of at least 85%. %, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more of the amino acid sequence identity of SEQ ID NO: 118;

(v) a nucleic acid which is an allelic variant or a splice variant of nucleic acid sequences according to (i) to (iv);

(vi) a portion of a nucleic acid sequence according to any of (i) to (v) above, which portion encodes a protein comprising: (i) CYP AaD domains; (ii) an N-terminal hydrophobic anchor domain; (iii) a transition domain; and (iv) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position, and preferably ascending at least 85%. %, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% with the amino acid sequence of SEQ ID NO: 118

In addition, the CYP90B polypeptide or homologue thereof may further comprise (i) a sequence of greater than 50% identity to SEQ ID NO: 78 and (ii) steroid 22 alpha hydroxylase enzymatic activity.

Expression of a nucleic acid encoding a CYP90B polypeptide or homologue thereof can be increased non-constitutively by introducing a genetic modification (preferably at the locus of a CYP90B gene). The locus of a gene as defined herein is adopted to mean a genomic region which includes the gene of interest and 10 kb before or after the coding region.

Genetic modification may be introduced, for example, by any one or more of the following methods: T-DNA activation, TILLING, site-directed mutagenesis, direct evolution and homologous recombination, or by introducing and expressing a nucleic acid encoding a plant. CYP90B polypeptide or a homologue thereof. The methods mentioned above are defined in the "Definitions" section. Following introduction of genetic modification, a step of selecting for increased non-constitutive expression of a nucleic acid encoding a CYP90B polypeptide or homologue thereof, whose increase in non-constitutive expression generates plants having increased yield, follows a step of selecting for genetic non-constitutive expression.

T-DNA activation, TILLING, site-directed mutagenesis, and direct evolution are examples of technologies that enable the generation of new CYP90B alleles and variants.

A preferred method for introducing a genetic modification (which in this case need not be at the locus of a CYP90B gene) is to introduce and express into a plant a nucleic acid encoding a CYP90B polypeptide or a homologue thereof. A CYP90B polypeptide or homologue thereof is defined as polypeptide comprising the following: (a) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position. The nucleic acid to be introduced into a plant may be a full length nucleic acid or may be a hybridizing moiety or sequence as defined above. In addition, the nucleic acid encoding a CYP90B polypeptide or homologue thereof may further comprise (i) a sequence with more than 50% identity to SEQ ID NO: 78 and (ii) steroid 22 alpha hydroxylase enzymatic activity.

"Protein homologues" are defined here in the "Definitions" section. The CYP90B polypeptide or homologue thereof may be a derivative as defined in the "Definitions" section.

The CYP90B polypeptide or homologue thereof may be encoded by an alternate binding variant of a CYP90B nucleic acid / gene. The term "alternate union variant" is defined in the "Definitions" section. Preferred splice variants are nucleic acid splice variants encoding a polypeptide comprising the following: (a) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, allowing an amino acid change at any position. Additionally, the CYP90B polypeptide or a homologue thereof may further comprise (i) a sequence with more than 50% identity to SEQ ID NO: 78 and (ii) steroid 22 alpha hydroxylase enzymatic activity. Still preferred are nucleic acid sequence splice variants represented by SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87 and SEQ ED NO: 89. Most preferred is a splice variant of a nucleic acid sequence as represented by SEQ ID NO: 77.

The homologue may also be encoded by an allelic variant of a nucleic acid encoding a CYP90B polypeptide or a homologue thereof, preferably an allelic variant of nucleic acid encoding a polypeptide comprising the following: (a) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser5 consensus sequence assuming an amino acid change at any position. Additionally, the CYP90B polypeptide or a homologue thereof may further comprise (i) a sequence with more than 50% identity to SEQ ID NO: 78 and (ii) steroid 22 alpha hydroxylase enzymatic activity. Still preferred are allelic variants of nucleic acid sequences represented by SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87 and SEQ ID Most preferred is an allelic variant of a nucleic acid sequence as represented by SEQ ID NO: 77. Allelic variants are also defined in the "Definitions" section.

In a preferred aspect of the present invention, increased non-constitutive expression of the CYP90B nucleic acid or variant thereof is contemplated. Methods for enhancing gene expression or gene products are well documented in the art and include, for example, overexpression directed by appropriate promoters, the use of transcriptional enhancers or translation enhancers. Isolated nucleic acids serving as promoter or enhancer elements may be introduced at an appropriate (typically before) position in a nonheterologous form of a polynucleotide in order to increase expression of a CYP90B nucleic acid or variant thereof. For example, endogenous promoters may be altered in vivo by mutation, deletion, and / or substitution (see, Kmiec, U.S. Patent No. 5,565,350; Zarling et al, PCT / US93 / 03868), or isolated promoters may be introduced into a plant cell at the appropriate orientation and distance of a gene of the present invention to control gene expression. Methods for reducing gene expression or gene products are well documented in the art.

If polypeptide expression is desired, it is generally desirable to include a polyadenylation region at the 3 'end of a polynucleotide coding region. The polyadenylation region may be derived from the natural gene, a variety of other plant genes, or T-DNA. The 3 'end sequence to be added may be derived, for example, from the nopaline synthase or octopine synthase genes, or alternatively from another plant gene, or less preferably from any other eukaryotic gene.

An intronic sequence can also be added to the untranslated 5 'region or coding sequence of the partial coding sequence to increase the amount of mature message that accumulates in the cytosol. Inclusion of a processable intron in the transcription unit in both plant and animal constructs has been shown to increase gene expression at both mRNA and protein levels by up to 1000-fold (Buchman and Berg (1988) Mol. Cell biol. 8: 4395-4405; Callis et (1987) Genes Dev 1: 1183-1200). Such intronic stimulation of gene expression is typically greater when located near the 5 'end of the transcription unit. Using the introns of Adhl-S 1, 2, and 6 intron maize, the Bronze-I intron are known in the art. See generally, The Maize Handbook, Chapter 116, Freeling and Walbot, Eds., Springer, N.Y. (1994).

The invention also provides genetic constructs and vectors to facilitate introduction and / or expression of nucleotide sequences useful in the methods according to the invention.

Therefore, a gene construct is provided comprising: (i) A CYP90B nucleic acid or variant thereof as defined above;

(ii) One or more control sequences capable of directing non-constitutive expression of the nucleic acid sequence of (i); and optionally

(iii) A transcription termination sequence.

Constructs useful in the methods according to the present invention may be constructed using recombinant DNA technology well known to those skilled in the art. Gene constructs may be inserted into commercially available vectors suitable for plant transformation and suitable for expression of the gene of interest in transformed cells. The invention therefore provides use of a gene construct as defined above in the methods of the invention.

Plants are transformed with a vector comprising the sequence of interest (i.e., a nucleic acid encoding a CYP90B polypeptide or homologue thereof). The sequence of interest is operably linked to one or more control sequences (at least one promoter). The terms "regulatory element", "control sequence" and "promoter" are all used interchangeably in this place and are defined in the "Definitions" section.

Advantageously, any non-constitutive type of promoter may be used to direct expression of the nucleic acid sequence. The non-constitutive promoter may be an inducible promoter, i.e. having induced or enhanced transcription initiation in response to a developmental, chemical, environmental or physical stimulus. An example of an inducible promoter being a stress-inducible promoter, i.e. a promoter activated when a plant is exposed to various stress conditions. The non-constitutive promoter may be a tissue preferred promoter, i.e. one which is capable of preferentially initiating transcription in certain tissues, such as leaves, roots, seed tissue, etc. Promoters capable of initiating transcription in certain tissues are referred to herein as "tissue specific" only.

In accordance with the methods of the invention, CYP90B nucleic acid or variant thereof is operably linked to a non-constitutive promoter. A non-constitutive promoter is transcriptionally active only during some phases of plant growth and development and is not ubiquitously expressed. The non-constitutive promoter may be for example a specific seed promoter, or a specific root promoter. The specific seed promoter may be a specific endosperm promoter and / or embryo / specific aleurone, i.e. transcriptionally active in the seed endosperm and / or embryo and seed aleurone, respectively. The specific endosperm promoter is preferably a seed storage protein promoter, yet preferably the specific endosperm promoter is a prolamine promoter, more preferably the specific endosperm promoter is a rice RP6 prolamine promoter, even more preferably the specific endosperm promoter is. represented by a nucleic acid sequence substantially similar to SEQ ID NO: 109, most preferably the specific endosperm promoter is as represented by SEQ ID NO: 109.

The embryo / specific aleurone promoter is preferably a seed storage protein promoter, yet preferably the embryo / specific aleurone promoter is an oleosin promoter, more preferably the embryo / specific aleurone promoter is an 18 kDa rice oleosin promoter, even more preferably the embryo / specific aleurone promoter is represented by a nucleic acid sequence substantially similar to SEQ ID NO: 110, most preferably the embryo / specific aleurone promoter is as represented by SEQ ID NO: 110. The specific root promoter is preferably an Rcc3 promoter, the specific root promoter is preferably a rice Rcc3 promoter (Xu et al. (1995) Plant Mol Biol 27 (2): 237-48).

It should be clear that the applicability of the present invention is not restricted to the CYP90B nucleic acid represented by SEQ ID NO: 77, nor is the applicability of the invention restricted to the expression of a CYP90B nucleic acid when directed by a PRP6 or oleosin promoter. kDa. Examples of other non-constitutive promoters that may also be used to perform the methods of the invention are shown in Table 4 in the "Definitions" section.

In contrast to the promoters described above, a constitutive promoter is transcriptionally active during most stages of plant growth and development and is substantially ubiquitously expressed in the plant. Such constitutive promoters should be excluded for performance of the methods of the invention. Examples of such promoters can also be found in the "Definitions" section (see Table 3).

Optionally, one or more terminator sequences may also be used in the construction introduced into a plant. The term "terminator" is defined in the "Definitions" section.

The genetic constructs of the invention may additionally include an origin of replication sequence that is required for maintenance and / or replication in a specific cell type. An example is when a genetic construct is required to be maintained in a bacterial cell as an episomal genetic element (e.g., plasmid or cosmid molecule). Preferred sources of replication include, but are not limited to, fl -ori and colEl.

The genetic construct may optionally comprise a selectable marker gene as defined in the "Definitions" section.

In a preferred embodiment, a gene construct is provided comprising: (i) A CYP90B nucleic acid or variant thereof as defined above;

(ii) A promoter capable of directing non-constitutive expression of the nucleic acid sequence of (i); and optionally

(iii) A transcription termination sequence.

The non-constitutive promoter is preferably a specific seed promoter. The specific seed promoter may be a specific endosperm promoter and / or embryo / specific aleurone, i.e. transcriptionally active in the seed endosperm and / or embryo and seed aleurone, respectively. The specific endosperm promoter is preferably a seed storage protein promoter, yet preferably the specific endosperm promoter is a prolamine promoter, more preferably the specific endosperm promoter is a rice RP6 prolamine promoter, more preferably the specific endosperm promoter is represented. by a nucleic acid sequence substantially similar to SEQ ID NO: 109, most preferably the specific endosperm promoter is as represented by SEQ ID NO: 109. The embryo / specific aleurone promoter is preferably a seed storage protein promoter, yet preferably the embryo / specific aleurone promoter is an oleosin promoter, more preferably the embryo / specific aleurone promoter is an 18 kDa rice oleosin promoter, more preferably the embryo / specific aleurone promoter is represented by a substantially similar nucleic acid sequenceSEQ ID NO: 110, most preferably the embryo / specific aleurone promoter is as represented by SEQ ID NO: 110. The invention further provides use of a construct as defined above in the methods of the invention.

The present invention also encompasses plants obtainable by the methods according to the present invention. The present invention therefore provides plants, plant parts or plant cells thereof obtainable by the method according to the present invention, which plants or parts or cells thereof comprise a transgenic CYP90B nucleic acid or variant thereof.

The invention also provides a method for producing transgenic plants having increased yield over suitable control plants comprising introducing and non-constitutive expression into a plant of a CYP90B nucleic acid or a variant thereof.

More specifically, the present invention provides a method for producing transgenic plants having increased yield which method comprises:

(i) introducing and constitutively expressing in a plant, plant part or plant cell a nucleic acid CYP90B or variant thereof; and

(ii) cultivate the plant cell under conditions promoting plant growth and development.

Nucleic acid can be introduced directly into a plant cell or into the plant itself (including introduction into a tissue, organ or any other part of a plant). According to a preferred feature of the present invention, the nucleic acid is preferably introduced into a plant by transformation.

The term "transformation" is as defined in the "Definitions" section.

The present invention clearly extends to any plant cell or plant produced by any of the methods described herein, and to all plant parts and seedlings thereof. The present invention further extends to encompass the progeny of a transformed or transfected primary cell, tissue, organ or whole plant that has been produced by any of the methods mentioned above, the sole requirement being that the progeny exhibit the same (s) genotypic and / or phenotypic trait (s) than those produced by the ancestor in the methods according to the invention.

The invention also includes host cells containing a single or non-constitutively expressed CYP90B nucleic acid expressed or variant thereof. Preferred host cells according to the invention are plant cells.

The invention also extends to harvestable parts of a plant such as, but not limited to seeds, leaves, fruits, flowers, stems, rhizomes, tubers and bulbs. The invention furthermore relates to products derived from a harvestable part of such a plant, such as dried pellets or powders, oil, fat and fatty acids, starch or proteins.

The present invention also encompasses use of CYP90B nucleic acids or variants thereof and use of CYP90B polypeptides or homologues thereof. Such uses refer to increasing crop yield as defined above in the methods of the invention.

CYP90B nucleic acids or variants thereof, or CYP90B polypeptides or homologues thereof may find use in breeding programs in which a DNA marker that can be genetically linked to a CYP90B gene or variant thereof is identified. Nucleic acids / CYP90B genes or variants thereof, or CYP90B polypeptides or homologues thereof may be used to define a molecular marker. This DNA or protein marker can then be used in breeding programs to select plants having increased yield as defined above in the methods of the invention. The CYP90B gene or variant thereof, for example, may be a nucleic acid as represented by any one of SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85 , SEQ ID NO: 87 and SEQ ID NO: 89.

Allelic variants of a CYP90B nucleic acid / gene may also find use in marker-assisted breeding programs. Such breeding programs sometimes require introduction of allelic variation by mutagenic treatment of plants, using for example EMS mutagenesis; alternatively, the program may start with a collection of allelic variants of the so-called "natural" origin caused unintentionally. Identification of allelic variants then occurs, for example, by PCR. This is followed by a step for selecting superior allelic variants of the sequence in question and generating increased production. Selection is typically performed by monitoring plant growth performance containing different allelic variants of the sequence in question, for example, allelic variants different from any of SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO : 83, SEQ ID NO: 85, SEQ ID NO: 87 and SEQ ID NO: 89. Growth performance can be monitored in a greenhouse or in the field. Additional optional steps include crossing plants in which the upper allelic variant has been identified with another plant. This can be used, for example, to make a combination of interesting phenotypic characteristics.

A CYP90B nucleic acid or variant thereof may also be used as probes to genetically and physically map the genes of which they are a part of, and as markers for traits linked to these genes. Such information may be useful in plant breeding in order to develop strains with desired phenotypes. Such use of CYP90B nucleic acids or variants thereof requires only a nucleic acid sequence of at least 15 nucleotides in length. CYP90B nucleic acids or variants thereof may be used as restriction fragment length polymorphism (RFLP) markers. Southern blots (Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning, A Laboratory Manual) of restriction digested plant genomic DNA can be probed with CYP90B nucleic acids or variants thereof. The resulting banding patterns can then be subjected to genetic analysis using computer programs such as MapMaker (Lander et al. (1987) Genomics 1: 174-181) in order to construct a genetic map. In addition, nucleic acids can be used to probe Southern blots containing restriction endonuclease-treated genomic DNAs from a set of individuals representing ancestor and progeny of a defined genetic crossover. Segregation of DNA polymorphisms is observed and used to calculate the position of CYP90B nucleic acid or variant on the genetic map previously obtained using this population (Botstein et al. (1980) Am. J. Hum. Genet. 32: 314-331) .

The production and use of plant gene derived probes for use in genetic mapping are described in Bematzky and Tanksley (1986) (GENETICS 112 (4): 887-898). Numerous publications describe genetic mapping of specific cDNA clones using the methodology described above or variations thereof. For example, F2 crossover populations, inbreeding populations, randomly crossed populations, nearby isogenic strains, and other sets of individuals may be used for mapping. Such methodologies are well known to those skilled in the art.

Nucleic acid probes may also be used for physical mapping (ie, location of sequences in physical maps; see Hoheisel et al. In: Non-mammalian Genomic Analysis: A Practical Guide, Academic press 1996, pp. 319-346, and references cited there).

In another embodiment, nucleic acid probes may be used in direct mapping by fluorescence in situ hybridization (FISH) (Trask (1991) Trends Genet. 7: 149-154). Although current FISH mapping methods favor the use of larger clones (several kb to several hundred kb; see Laan et al. (1995) Genome Res. 5: 13-20), sensitivity improvements may allow FISH mapping performance using probes. smaller ones.

A variety of nucleic acid amplification based methods for genetic and physical mapping can be performed using nucleic acids. Examples include allele-specific amplification (Kazazian (1989) J. Lab. Clin. Med. 11: 95-96), PCR amplified fragment polymorphism (CAPS; Sheffield et al. (1993) Genomics 16: 325-332), allele binding specific (Landegren et al. (1988) Science 241: 1077-1080), nucleotide extension reactions (Sokolov (1990) Nucleic Acid Res. 18: 3671), Radiation Hybrid Mapping (Walter et al. (1997) Nat Genet. 7: 22-28) and Appropriate Mapping (Dear and Cook (1989) Nucleic Acid Res. 17: 6795-6807). For these methods, the nucleic acid sequence is used to design and produce primer pairs for use in the amplification reaction or primer extension reactions. The design of such initiators is well known to those skilled in the art. In methods employing PCR-based genetic mapping, it may be necessary to identify DNA sequence differences between the mapping ancestors in the region corresponding to the immediate sequence of nucleic acids. This, however, is generally not required for mapping methods.

The methods according to the present invention result in plants having increased yield as described above. This increased yield can also be combined with other economically advantageous features, such as additional features that stimulate yield, tolerance to other abiotic and biotic stresses, features modifying various architectural attributes and / or biochemical and / or physiological attributes.

Detailed Description CDC27

CDC27 polypeptides are well known in the art and are readily identifiable by the presence of a conserved NH2 terminal region (see Figure 16) and at least 5 TPR domains with at least one TPR domain in the NH2 terminal region. In addition, the CDC27 polypeptide may further comprise a sequence with more than 30% identity with SEQ ID NO: 130.

TPR motifs are present in a wide variety of yeast functional proteins and higher mitosis eukaryotes (including the APC protein components CDC16, CDC23 and CDC27), transcription, processing, protein importation and neurogenesis (Goebl and Yanagida 1991, Trends Biochem Sci 16, 173-177). A suggested minimum consensus sequence of the TPR motif is: X3-W-X2-LG-X2-Y-Xs-A-X3-F-X2-A-X4-P-X2, where X = any amino acid (Lamb et al. 1994, EMBO J 13, 4321-4328). Consensus residues may exhibit significant degeneration and non-consensus residues exhibit little or no homology. It is the hydrophobicity and size of the consensus waste, rather than its identity, that seems to be important. In a native CDC27 protein, TPR forms an α-helical structure, tandem repeats organize into a superhelical structure ideally suited as protein recognition interfaces (Groves and Barford 1999, Curr Opin Struct Biol 9, 383-389) . Within the α-helix, two amphipathic domains are usually present, one in the NH2 terminal region and the other near the COOH-terminal region (Sikorski et al. 1990, Cell 60, 307-317). Also individual TPR motifs may be scattered throughout the protein sequence.

A full length native CDC27 typically comprises at least 5 TPRs, preferably 6 TPRs, more preferably 7 TPRs, most of these TPRs being located in the terminal COOH region. As shown in Figure 16, there is typically a TPR domain in the terminal NH2 region of a native CDC27 polypeptide, although CDC27 variant sequences may exist or may be created to comprise more than one TPR in the terminal NH2 region. Any CDC27 polypeptide may be made useful in the methods of the invention by inactivating at least one TPR domain in the terminal NH2 region of the polypeptide. Methods for inactivation are well known in the art and include: amino acid removal or substitution, in this case amino acid removal or substitution of at least one TPR domain in the terminal NH2 region; or mutation techniques, such as replacing alanine-conserved amino acids or substituting phosphorylable amino acids (such as serine, threonine or tyrosine) with non-phosphorylatable amino acids or vice versa (depending on whether the phosphorylated protein is active or inactive); or any other method for inactivation.

For the purposes of this application, the terminal NH2 region of a CDC27 protein is considered to be the first half of a full length CDC27 sequence (from terminal NH2 to terminal COOH) (see Figure 16); preferably the terminal NH2 region of a CDC27 protein is considered to be the first third of a full length CDC27 sequence (from terminal NH2 to terminal COOH); and according to another preferred feature of the present invention, the N-terminal region of a CDC27 protein is considered to be the first 166 amino acids (from terminal NH2 to terminal COOH) of a full length CDC27 sequence.

An example of a CDC27 polypeptide having at least one inactive TPR domain in the terminal NH2 region is the polypeptide represented by SEQ ID NO: 130, with the encoding nucleic acid sequence represented by SEQ ID NO: 129.

Table 10 below provides some examples of CDC27 sequences; These sequences may be made useful in the methods of the invention by inactivating at least one TPR domain in the terminal NH2 region of the polypeptide, for example using any of the inactivation methods discussed above. Table 10: Examples of CDC27 Polypeptides

<table> table see original document page 116 </column> </row> <table>

Sequence grouping compiled from several EST accesses (the main ones shown); If EST sequencing quality is usually inferior, a few nucleic acid substitutions can be expected.

The sequences described in Table 10 are given by way of example only. Additional examples are given in Figure 19, encoding either full length or partial polypeptides (which can be used to obtain the full length sequence using routine methods). It should be understood that any CDC27 polypeptide sequence having at least one inactive TPR domain in the terminal NH2 region of the polypeptide, or a nucleic acid / gene encoding such a polypeptide, may be suitable for use to perform the methods of the invention.

Other CDC27 polypeptides may be readily identified using routine techniques well known in the art, such as by sequence alignment. Sequences thus identified may subsequently be made useful in the methods of the invention by inactivating at least one TPR domain in the terminal NH2 region of the polypeptide, for example using any of the inactivation methods discussed above. Methods for sequence alignment for comparison are well known in the art, such methods include GAP, BESTFIT, BLAST, FASTA and TFASTA. GAP uses the algorithm of Needleman and Wunsch ((1970) J Mol Biol 48: 443-453) to find the alignment of two complete sequences that maximizes the number of identities and minimizes the number of gaps. The BLAST algorithm (Altschul et al. (1990) J Mol Biol 215: 403-10) calculates percent sequence identity and performs a statistical analysis of similarity between the two sequences. The computer application for performing BLAST analysis is publicly available through the National Center for Biotechnology Information. CDC27 homologs can be readily identified using, for example, the ClustalW multiple sequence alignment algorithm (version 1.83) available at http://clustalw.genome.jp/sit-bin/nph-ClustalW, with the default parameters of paired alignment, and a percentage scoring method. Secondary manual editing can be performed to optimize alignment between retained subjects, as should be apparent to a person skilled in the art.

Several structural domains in a CDC27 protein, such as TPR domains, can be identified using specialized databases eg SMART (Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864; Letunic et al. (2002) Nueleie Aeids Res 30, 242-244; http://smart.embl-heidelberg.de/), InterPro (Mulder et al., (2003) Nucl. Acids Res. 31, 315-318; http: //www.ebi.ae.uk/interpro/), Prosite (Bucher and Bairoeh (1994), A generalized profile syntax for biomolecular sequences motifs and its funetion in automatic sequence interpretation. (In) ISMB-94; Proceedings 2nd International Conferenee on Intelligent Systems for Molecular Biology Altman R., Brutlag D., Karp P., Lathrop R., Searls D., Eds., pp53-61, AAAIPress, Menlo Park; Hulo et al., Nucl. Acids Res. 32.-D134-D137, (2004), http://www.expasy.org/prosite/), Pfam (Bateman et al., Nucleic Acids Research 30 (1): 276-280 (2002), http: / /www.sanger.ac.uk/Software/Pfam/) or ProDom (Servant F, Bru C, Carrère S, Courcelle E, Gouzy J, Peyruc D, Kahn D (2002) ProDom:

Automated clustering of homologous domains. Briefings in Bioinformatics. vol 3, no 3: 246-251).

The sequences mentioned in Table 10 and Figure 19 can be considered homologous to a CDC27 polypeptide. "Counterparts" of a protein are defined in the "Definitions" section in this place. Preferred homologues are amino acid sequences having in ascending order preferably at least 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% or more sequence identity to the full length CDC27 protein represented by SEQ ID NO: 132.

Homologs, orthologs and paralogs may be made useful in the methods of the invention by inactivating at least one TPR domain in the terminal NH2 region of the polypeptide, for example using any of the inactivation methods discussed above.

Human and yeast CDC27 polypeptides have been shown to interact with two other APC complex proteins, CDC16 and CDC23, in vivo by analysis of two yeast hybrids, and in vitro by co-immunoprecipitation (Lam et al. (1994) EMBO J 13 (18): 4321-4328; Ollendorf & Donoghue (1997) J Biol Chem 272 (51): 32011-32018). Such an interaction may be useful for identifying CDC27 polypeptides to be made useful in the methods of the invention by inactivating at least one TPR domain in the NH2 terminal region of the polypeptide, for example using any of the inactivation methods discussed above.

A CDC27 polypeptide having at least one inactive TRP domain in the NH2 terminal region of the polypeptide is encoded by a so-called nucleic acid / modified CDC27 gene. Therefore, the term "modified CDC27 nucleic acid / gene" as defined herein is any nucleic acid / gene encoding a CDC27 polypeptide having at least one inactive TRP domain in the NH2 terminal region of the polypeptide.

The CDC27 nucleic acid or modified CDC27 nucleic acid / gene may be derived from any natural or artificial source. The nucleic acid / gene may be isolated from a microbial source, such as yeast or fungi, or from a plant, algal or animal source. This nucleic acid may be modified from its native form in genomic composition and / or environment through deliberate human manipulation. The nucleic acid is preferably of plant origin, either from the same plant species (for example that into which it is introduced) or from a different plant species. The nucleic acid may be isolated from a dicotyledonous species, preferably from the Brassicaceae family, still preferably from Arabidopsis thaliana. More preferably, the modified CDC27 nucleic acid isolated from Arabidopsis thaliana is represented by SEQ ID NO: 129 and the CDC27 having at least one inactive TPR in the amino acid terminal NH2 region is as represented by SEQ ID NO: 130.

A CDC27 nucleic acid / gene is a nucleic acid capable of hybridizing under reduced stringency conditions, preferably under stringent conditions, to a CDC27 nucleic acid / gene as represented by any of SEQ ID NO: 129, SEQ ID NO: 131, SEQ SEQ ID NO: 135, SEQ ID NO: 137 or SEQ ID NO: 141. More preferably the hybridizing sequence is one which is capable of hybridizing to a nucleic acid as represented by SEQ ID NO: 129 or SEQ ID NO: 131. Such hybridizing sequences may be made useful in the methods of the invention by inactivating at least one TPR domain in the NH2 terminal region of the encoded polypeptide, for example using any of the inactivation methods discussed above.

The term "hybridization" is as defined here in the "Definitions" section.

The CDC27 nucleic acid or modified CDC27 nucleic acid / gene may be in the form of an alternative splicing variant. An alternative union variant is defined in the "Definitions" section. Splice variants of any of the above mentioned CDC27 nucleic acid sequences are preferred, in other words SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137 or SEQ Most preferred is a splice variant of a nucleic acid sequence as represented by SEQ ID NO: 129 or SEQ ID NO: 131. Such splice variants may be made useful in inactivation methods of the invention of at least at least one TPR domain in the NH2 terminal region of the encoded CDC27 polypeptide, for example using any of the inactivation methods discussed above.

The CDC27 nucleic acid or modified CDC27 nucleic acid / gene may be in the form of an allelic variant of a nucleic acid encoding a truncated CDC27 polypeptide comprising at least one inactivated TPR domain in the terminal NH2 region. Allelic variants of nucleic acid sequences represented by SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137 or SEQ ID NO: 141 are preferred. is an allelic variant of a nucleic acid sequence as represented by SEQ ID NO: 129 or SEQ ID NO: 131. Allelic variants exist in nature, and encompassed within the methods of the present invention is the use of these natural alleles. Allelic variants include Single Nucleotide Polymorphisms (SNPs), as well as Small Insert / Deletion Polymorphisms (INDELs). The size of INDELs is typically less than 100 bp. SNPs and INDELs form the largest set of sequence variants in naturally occurring polymorphic strains of most organisms. Such allelic variants may be made useful in the inactivation methods of the invention of at least one TPR domain in the NH2 terminal region of the encoded CDC27 polypeptide, for example using any of the inactivation methods discussed above.

The CDC27 nucleic acid or modified CDC27 nucleic acid / gene may be generated by site directed mutagenesis. Several methods are available to achieve site directed mutagenesis, the most common being PCR-based methods (Current Protocols in Molecular Biology, Wiley Eds http://www.4ulr.com/products/currentprotocols/index.html).

CDC27 nucleic acid or modified nucleic acid / CDC27 gene can also be generated by direct evolution (see section "Definitions" for further details).

Such variants produced by site-directed mutagenesis or direct evolution may be made useful in the inactivation methods of the invention of at least one TPR domain in the NH2 terminal region of the encoded CDC27 polypeptide, for example using any of the inactivation methods discussed above.

Expression of a modified CDC27 nucleic acid / gene encoding a CDC27 polypeptide having at least one inactive TPR domain in the NH2 terminal region of the polypeptide may be enhanced by introducing a genetic modification (preferably at the locus of a CDC27 gene). The locus of a gene as defined herein is adopted to mean a genomic region, which includes the gene of interest and 10 KB before or after the coding region.

Genetic modification is preferably introduced by introducing and expressing into a plant a nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the terminal NH2 region of the polypeptide. Following introduction of the genetic modification, an additional step of selecting for increased expression (in apical bud meristem tissue) of a modified nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the terminal NH2 region of the polypeptide, the increase of which is increased. expression generates plants having increased yield.

According to a preferred aspect of the present invention, increased expression of CDC27 nucleic acid is contemplated. Methods for enhancing gene expression or gene products are well documented in the art and include, overexpression directed by appropriate promoters, the use of transcription enhancers or translation enhancers. Isolated nucleic acids serving as promoter or enhancer elements may be introduced at an appropriate (typically before) position in a nonheterologous form of a polynucleotide in order to enhance expression of a CDC27 nucleic acid. For example, endogenous promoters may be altered in vivo by mutation, deletion, and / or substitution (see, Kmiec, U.S. Patent No. 5,565,350; Zarling et al., PCT / US93 / 03868), or isolated promoters. be introduced into a plant cell at the appropriate orientation and distance of a gene of the present invention to control gene expression.

If polypeptide expression is desired, it is generally desirable to include a polyadenylation region at the 3 'end of a polynucleotide coding region. The polyadenylation region may be derived from the natural gene, a variety of other plant genes, or T-DNA. The 3 'end sequence to be added may be derived, for example, from the nopaline synthase or octopine synthase genes, or alternatively from another plant gene, or less preferably from any other eukaryotic gene.

An intronic sequence can also be added to the untranslated 5 'region or coding sequence of the partial coding sequence to increase the amount of mature message that accumulates in the cytosol. Inclusion of a processable intron in the transcription unit in both plant and animal constructs has been shown to increase gene expression at both mRNA and protein levels by up to 1000-fold (Buchman and Berg (1988) Mol. Cell biol. 8: 4395-4405; Callis et (1987) Genes Dev 1: 1183-1200). Such intronic stimulation of gene expression is typically greater when located near the 5 'end of the transcription unit. Using the introns of maize Adhl-S 1, 2, and 6, the Bronze-1 intron are known in the art. See generally, The Maize Handbook, Chapter 116, Freeling and Walbot, Eds., Springer, N.Y. (1994).

The invention also provides genetic constructs and vectors to facilitate introduction and / or expression of nucleotide sequences useful in the methods according to the invention.

Therefore, a gene construct is provided comprising:

(i) A CDC27 nucleic acid encoding a CDC27 polypeptide having at least one inactivated TPR domain in the NH2 terminal region of the polypeptide;

(ii) One or more control sequences capable of preferentially directing expression of the nucleic acid sequence of (i) in bud apical meristem tissue; and optionally

(iii) A transcription termination sequence.

Such genetic constructs can be constructed using recombinant DNA technology well known to those skilled in the art. Gene constructs may be inserted into commercially available vectors suitable for plant transformation and suitable for expression of the gene of interest in transformed cells. The invention therefore provides use of a gene construct as defined above in the methods of the invention.

Plants are transformed with a vector comprising the sequence of interest (i.e., a nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the NH2 terminal region of the polypeptide). The sequence of interest is operably linked to one or more control sequences (at least one promoter) capable of preferentially directing expression in plant apical bud meristem tissue. The terms "regulatory element", "control sequence" and "promoter" are all used interchangeably in this place and are defined in the "Definitions" section.

The CDC27 nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the terminal NH2 region of the polypeptide or variant is operably linked to a bud apical meristem promoter, preferably to an early bud apical meristem promoter. An "early bud apical meristem promoter" as defined herein is a promoter that is transcriptionally active on the bud apical meristem from the globular embryo stage to the young seedling stage, these stages being well known to those skilled in the art. Reference herein to preferentially increasing expression in bud apical meristem tissue is adopted to mean increasing expression in bud apical meristem tissue substantially to the exclusion of expression elsewhere in the plant, without considering any residual expression due to permeable promoters. Preferably, the early sprout apical meristem promoter is a rice OSH1 promoter; SEQ ID NO: 151 (Matsuoka et al., (1993) Plant Cell 5: 1039-1048; Sato et al., (1996) Proc Natl). Acad Sci USA 93 (15): 8117-22)). It should be clear that the applicability of the present invention is not restricted to the modified CDC27 nucleic acid represented by SEQ ID NO: 129, nor is the applicability of the invention restricted to the expression of a modified CDC27 nucleic acid when directed by an OSH1 promoter. Examples of other early bud apical meristem promoters are shown in Table 5 in the "Definitions" section. These are members of class 1 homeobox of the KNOX family, of paralogue genes or orthologs. It should be understood that the list below is not exhaustive.

Optionally, one or more terminator sequences may also be used in the construction introduced into a plant. The term "terminator" is defined here in the "Definitions" section.

The genetic constructs of the invention may additionally include an origin of replication sequence that is required for maintenance and / or replication in a specific cell type. An example is when a genetic construct is required to be maintained in a bacterial cell as an episomal genetic element (e.g., plasmid or cosmid molecule). Preferred sources of replication include, but are not limited to, fl-ori and colEl.

The genetic construct may optionally comprise a selectable marker gene as defined in the "Definitions" section.

The present invention also encompasses plants obtainable by the methods according to the present invention. The present invention therefore provides plants or parts thereof, including plant cells, obtainable by the method according to the present invention, which plants or plant parts comprise a CDC27 nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the terminal NH2 region of the polypeptide and whose nucleic acid is operably linked to a bud apical meristem promoter.

The invention also provides a method for producing transgenic plants having increased seed numbers relative to suitable control plants, comprising introducing and expressing in a plant a CDC27 nucleic acid encoding a CDC27 polypeptide having at least one TPR domain inactive in the region. Terminal NH2 of the polypeptide, whose CDC27 nucleic acid is under the control of a bud apical meristem promoter.

More specifically, the present invention provides a method for the production of transgenic plants having increased number of seeds over suitable control plants, which method comprises:

(i) introducing and expressing into the plant, plant part or plant cell a CDC27 nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the NH2 terminal region of the polypeptide, whose nucleic acid is under the control of an apical meristem promoter. sprout; and

(ii) cultivate the plant cell under conditions promoting plant growth and development.

Nucleic acid can be introduced directly into a plant cell or into the plant itself (including introduction into a tissue, organ or any other part of a plant). According to a preferred feature of the present invention, the nucleic acid is preferably introduced into a plant by transformation.

The term "transformation" is defined in the "Definitions" section.

The present invention clearly extends to any plant cell or plant produced by any of the methods described herein, and to all plant parts and seedlings thereof. The present invention further extends to encompass the progeny of a transformed or transfected primary cell, tissue, organ or whole plant that has been produced by any of the methods mentioned above, the sole requirement being that the progeny exhibit the same (s) genotypic and / or phenotypic trait (s) than those produced by the ancestor in the methods according to the invention.

The invention also includes host cells containing an isolated CDC27 nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the NH2 terminal region of the polypeptide and whose nucleic acid is under the control of a bud apical meristem promoter. Preferred host cells according to the invention are plant cells.

The invention also extends to harvestable parts of a plant such as, but not limited to seeds, leaves, fruits, flowers, stems, rhizomes, tubers and bulbs. The invention further relates to products derived, preferably directly derived, from a crop-fit part of such a plant, such as dried pellets or powders, oil, fat and fatty acids, starch or proteins.

The present invention also encompasses use of CDC27 nucleic acids encoding CDC27 polypeptides having at least one inactive TPR domain in the terminal NH2 region of the polypeptide, whose nucleic acids are under the control of a bud apical meristem promoter. Such uses refer to increasing crop yield as defined above in the methods of the invention.

Performance of the methods according to the present invention results in plants having increased seed numbers relative to suitable control plants. This increase in seed number can also be combined with other economically advantageous features, such as additional features that stimulate yield, tolerance to other abiotic and biotic stresses, features modifying various architectural attributes and / or biochemical and / or physiological attributes. AT-hook Detailed Description

AT-hook domains are well known in the art and are typically found on polypeptides belonging to a family of transcription factors associated with Chromatin remodeling. The AT-hook motif consists of 13 or more (sometimes about 9) amino acids that participate in DNA binding and have a preference for A / T-rich regions. In Arabidopsis there are at least 34 proteins containing AT-hook domains. These proteins share homology throughout most of the sequence, with the AT-hook domain being a particularly highly conserved region. The AT-hook domain is illustrated in Figure 23 and Table II below; see also the appropriate notation of SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169 and SEQ ID NO: 171 where the position of the AT-hook domain is specified. As shown in the alignment of Figure 23, some variation within the AT-hook domain is allowed. Typically, one or two AT-hook domains precede the DUF296 domain. Reference herein to an AT-hook domain is adopted to mean a sequence of polypeptides having in ascending order of preference at least 70%, 75%, 80%, 85%, 90% or 95% identity with the AT-hook domain. of SEQ ID NO: 153, which is repeated here for convenience: RRPRGRPAGSKNK (AT-hook domain of SEQ ID NO: 153).

DUF296 domains (referred to in Interpro as IPR005175) are also well known in the art. The domain DUF296 is illustrated in Figure 23 and Table II below; see also the appropriate notation of SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169 and SEQ ID NO: 171, where the domain position DUF296 is specified. As shown in the alignment of Figure 23, variation within the DUF296 domain is allowed while still being easily identified as a DUF296 domain due to the presence of some highly conserved amino acid residues. Typically, the DUF296 domain is preceded by one or two AT-hook domains.

According to a preferred feature of the present invention, polypeptides comprising an AT-hook domain and a DUF296 domain further comprise one of the following reasons:

Reason 1 (SEQ ID NO: 190): QGQ V / I GG; or

Reason 2 (SEQ ID NO: 191): ILSLSGSFLPPPAPP; or

Reason 3 (SEQ ID NO: 192): NATYERLP; or

Reason 4 (SEQ ID NO: 193): SFTNVAYERLPL with none or an amino acid change at any position; or

Reason 5 (SEQ ID NO: 194): GRFEILSLTGSFLPGPAPPGSTGLTIYLAGGQGQWGGSVVG with no, one or two amino acid changes at any position.

According to a preferred feature of the present invention, sequences suitable for use in the methods of the invention are polypeptides comprising an AT-hook domain (as defined above) and a DUF296 domain (as defined above) and Reason 2 (as defined above), or nucleic acids encoding such polypeptides.

It should be understood that the sequences detailed in Table 1 and those shown in the alignment of Figure 23 are only examples of sequences useful in the methods of the invention and that any polypeptide having an AT-hook domain and a DUF296 domain, or any nucleic acid encoding the same. may be suitable for use to perform the methods of the invention.

Table 11: Examples of amino acid sequences comprising an AT-hook domain and a DUF296 domain with details of the sequences of these domains and their respective positions.

<table> table see original document page 129 </column> </row> <table> <table> table see original document page 130 </column> </row> <table> <table> table see original document page 131 < / column> </row> <table>

One skilled in the art will readily be able to identify polypeptides comprising an AT-hook domain and a DUF296 domain using techniques and tools well known in the art. Such identification may be by sequence alignment for sequence comparison using GAP, BESTFIT, BLAST, FASTA and TFASTA. GAP uses the algorithm of Needleman and Wunsch ((1970) J Mol Biol 48: 443-453) to find the alignment of two complete sequences that maximizes the number of identities and minimizes the number of gaps. The BLAST algorithm (Altschul et al. (1990) J Mol Biol 215: 403-10) calculates percent sequence identity and performs a statistical analysis of similarity between the two sequences. The computer application for performing BLAST analysis is publicly available through the National Center for Biotechnology Information. Polypeptides comprising an AT-hook domain and a DUF296 domain can be readily identified using, for example, the ClustalW multiple sequence alignment algorithm (version 1.83) available at http://clustalw.genome.jp/sit-bin/nph- ClustalW, with the standard paired alignment parameters, and a percentage scoring method. Secondary manual editing can be performed to optimize alignment between retained subjects, as should be apparent to a person skilled in the art.

The AT-hook domain and the DUF296 domain can be identified using specialized databases eg SMART (Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864; Letunic et al. (2002) Nucleic Acids Res 30, 242-244; http://smart.embl-heidelberg.de/), InterPro (Mulder et al., (2003) Nucl. Acids Res. 31, 315-318; http: // www. ebi.ac.uk/interpro/), Prosite (Bucher and Bairoch (1994), A generalized profile syntax for biomolecular sequence motifs and its funetion in automatic sequence interpretation. (In) ISMB-94; Proceedings 2nd International Conference on Intelligent Systems for Molecular Biology Altman R., Brutlag D., Karp P., Lathrop R., Searls D., Eds., Pp53-61, AAAIPress, Menlo Park; Hulo et al., Nucl. Acids Res. 32: D134- D137, (2004), http://www.expasy.org/prosite/) or Pfam (Bateman et al., Nucleic Acids Research 30 (1): 276-280 (2002), http: //www.sanger. ac.uk/Software/Pfam/).

The sequences mentioned in Table 11, or as identified using the techniques mentioned above (such as sequence alignment), may be considered homologous to a polypeptide comprising an AT-hook domain and a DUF296 domain, whose homologues also comprise an AT-hook domain. and a domain DUF296 but which may vary anywhere else in the sequence. "Counterparts" of a protein are defined in the "Definitions" section in this place. Preferred homologues are amino acid sequences having in ascending order preferably at least 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% or more of sequence identity with an amino acid sequence represented by SEQ ED NO: 153, which homologues comprise an AT-hook domain and a DUF296 domain and still preferably comprise Motive 2.

The polypeptide comprising an AT-hook domain and a DUF296 domain, or a homologue of such a polypeptide, may be a derivative as defined in the "Definitions" section herein.

Any nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain may be suitable for use in the methods of the invention. Examples of such sequences include those nucleotide sequences represented by SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO. : 164, SEQ ID NO: 166, SEQ ID NO: 168 and SEQ ID NO: 170.

Variants of a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain may also be suitable for use to practice the methods of the invention as long as variants encode polypeptides comprising an AT-hook domain and a DUF296 domain. Such nucleic acid variants may be portions of a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain and / or nucleic acids capable of hybridizing to a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain. .

A portion may be prepared, for example, by making one or more deletions to a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain. The portions may be used in isolation or they may be fused to other coding (or non-coding) sequences to, for example, produce a protein that combines various activities. When fused to other coding sequences, the resulting translation-produced polypeptide may be larger than that predicted for the portion. Preferably, the moiety is a portion of a nucleic acid as represented by any one of SEQ ID NO: 152, SEQID NO: 154, SEQID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162 SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168 and SEQ ID NO: 170. More preferably the portion is a portion of a nucleic acid as represented by SEQ ID NO: 152, which portion encodes a polypeptide. comprising an AT-hook domain and a DUF296 domain and further preferably comprising Reason 2.

Another nucleic acid variant is a nucleic acid capable of hybridizing under reduced stringency conditions, preferably under stringent conditions, to a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain. Preferably, the hybridizing sequence is one which is capable of hybridizing to a nucleic acid as represented by any of SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168 and SEQ ID NO: 170, or with a portion of any of the sequences mentioned above as defined above. More preferably, the hybridizing sequence is one that is capable of hybridizing to a nucleic acid as represented by SEQ ID NO: 152, whose hybridizing sequence encodes a polypeptide comprising an AT-hook domain and a DUF296 domain and still preferably comprises Motive 2.

The term "hybridization" is as defined here in the section

"Definitions".

Another nucleic acid variant is an alternative splice variant as defined in the "Definitions" section. Nucleic acid sequence splice variants represented by SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 162, SEQ ID are preferred. NO: 164, SEQ ID NO: 166, SEQ ID NO: 168 and SEQ ID NO: 170. Most preferred is a splice variant of a nucleic acid sequence as represented by SEQ ID NO: 152, whose splice variant encodes a polypeptide comprising an AT-hook domain and a DUF296 domain and still preferably comprising Motive 2.

Another nucleic acid variant is an allelic variant as defined in the "Definitions" section. Allelic variants of nucleic acid sequences represented by SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO are preferred. : 164, SEQ ID NO: 166, SEQ ID NO: 168 and SEQ ID NO: 170. Most preferred is an allelic variant of a nucleic acid sequence as represented by SEQ ID NO: 152, whose allelic variant encodes a polypeptide comprising an AT-hook domain and a DUF296 domain and further preferably comprises Reason 2.

Nucleic acid variants can also be obtained through direct evolution (see section "Definitions").

Site directed mutagenesis can also be used to generate variants of a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain. See section "Definitions".

Nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain may be derived from any natural or artificial source. The nucleic acid / gene or variant thereof may be isolated from a microbial source, such as yeast or fungi, or from a plant, algal or animal source. This nucleic acid may be modified from its native form in genomic composition and / or environment through deliberate human manipulation. The nucleic acid is preferably of plant origin, either from the same plant species (for example that into which it is introduced) or from a different plant species. The nucleic acid may be isolated from a dicotyledonous species, preferably from a monocotyledonous species such as rice. More preferably, rice nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain is represented by SEQ ID NO: 152 and the encoded polypeptide is as represented by SEQ ID NO: 153.

Expression of a nucleic acid encoding AT-hook may be modulated by introducing a genetic modification (preferably at the locus of a gene encoding a polypeptide comprising an AT-hook domain and a DUF296 domain). The locus of a gene as defined herein is adopted to mean a genomic region, which includes the gene of interest and 10 kb before or after the coding region.

Genetic modification may be introduced, for example, by any (or more) of the following methods: T-DNA activation, TILLING, homologous recombination and introducing and expressing into a monocot plant a nucleic acid encoding a polypeptide comprising an AT- domain. hook is a DUF296 domain. See the "Definitions" section for details of T-DNA activation, TILLING, and homologous recombination. Following introduction of genetic modification, a step of selecting for increased expression in endosperm tissue of a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain, whose targeted expression generates plants having increased seed production, can be followed.

The choice of promoter for identification by T-DNA activation in the case of the present invention may be any promoter capable of preferentially directing expression in endosperm tissue of a monocotyledonous plant.

T-DNA activation and TILLING are examples of technologies that enable the generation of novel alleles and variants of a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain.

A preferred method for introducing a genetic modification (which in this case need not be at the locus of a polypeptide-encoding nucleic acid / gene comprising an AT-hook domain and a DUF296 domain) is to introduce and express into a plant a polypeptide-encoding nucleic acid comprising an AT-hook domain and a DUF296 domain. The nucleic acid to be introduced into a plant may be a full length nucleic acid or may be a portion or any other variant nucleic acid as long as the variant nucleic acid encodes a polypeptide comprising an AT-hook domain and a DUF296 domain.

The methods of the present invention resort to preferentially increasing endosperm tissue expression of a monocotyledonous plant of a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain. This can be achieved by overexpression directed by appropriate promoters, the use of transcription enhancers or translation enhancers. Isolated nucleic acids serving as promoter or enhancer elements may be introduced at an appropriate (typically before) position in a nonheterologous form of a polynucleotide in order to enhance expression of a gene / nucleic acid or variant thereof encoding a polypeptide comprising an AT domain -hook and a domain DUF296. For example, endogenous promoters may be altered in vivo by mutation, deletion, and / or substitution (see, Kmiec, U.S. Patent No. 5,565,350; Zarling et al., PCT / US93 / 03868), or isolated promoters. be introduced into a plant cell at the appropriate orientation and distance of a gene of the present invention to control gene expression.

If polypeptide expression is desired, it is generally desirable to include a polyadenylation region at the 3 'end of a polynucleotide coding region. The polyadenylation region may be derived from the natural gene, a variety of other plant genes, or T-DNA. The 3 'end sequence to be added may be derived, for example, from the nopaline synthase or octopine synthase genes, or alternatively from another plant gene, or less preferably from any other eukaryotic gene.

An intronic sequence can also be added to the untranslated 5 'region or coding sequence of the partial coding sequence to increase the amount of mature message that accumulates in the cytosol. Inclusion of a processable intron in the transcription unit in both plant and animal constructs has been shown to increase gene expression at both mRNA and protein levels by up to 1000-fold (Buchman and Berg (1988) Mol. Cell biol. 8: 4395-4405; Callis et (1987) Genes Dev 1: 1183-1200). Such intronic stimulation of gene expression is typically greater when located near the 5 'end of the transcription unit. Using the introns of Adhl-S 1, 2, and 6 intron maize, the Bronze-I intron are known in the art. See generally, The Maize Handbook, Chapter 116, Freeling and Walbot, Eds., Springer, N.Y. (1994).

The invention also provides genetic constructs and vectors to facilitate introduction and / or expression of nucleotide sequences useful in the methods according to the invention.

Therefore, a gene construct is provided comprising:

(i) A nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain;

(ii) One or more control sequences capable of directing expression of the nucleic acid sequence of (i) in endosperm tissue of a monocotyledonous plant; and optionally

(iii) A transcription termination sequence. The invention also provides use of a construct as defined above in methods for increasing seed production of a monocot plant.

Constructs useful in the methods according to the present invention may be constructed using recombinant DNA technology well known to those skilled in the art. Gene constructs may be inserted into commercially available vectors suitable for plant transformation and suitable for expression of the gene of interest in transformed cells. The invention also provides use of a construct as defined above in methods for increasing seed yield in a monocot plant.

Monocotyledonous plants are transformed with a vector comprising the sequence of interest (i.e., a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain). The sequence of interest is operably linked to one or more control sequences (at least one promoter) capable of preferentially increasing expression in endosperm tissue of a monocotyledonous plant. The terms "regulatory element", "control sequence" and "promoter" are all used interchangeably in this place and are defined in the "Definitions" section.

A specific endosperm promoter refers to any promoter capable of preferentially directing expression of the gene of interest in endosperm tissue. Reference herein to preferentially increase endosperm tissue expression is adopted to mean increasing endosperm tissue expression substantially to the exclusion of expression elsewhere in the plant, without considering any residual expression due to permeable promoters. For example, the prolamine promoter shows strong endosperm expression with meristem permeability, more specifically the bud meristem and / or center of discrimination in the meristem. Preferably, the specific endosperm promoter is an isolated promoter of a prolamine gene, such as a rice RP6 prolamine promoter (Wen et al, (1993) Plant physiol 101 (3): 1115-6) as represented by SEQ ID NO. : 195 or a similar strength promoter and / or a promoter with a similar expression pattern as the rice prolamine promoter. Similar force and / or similar expression pattern can be analyzed, for example, by coupling promoters to a reporter gene and by checking reporter gene function in plant tissues. A well-known reporter gene is beta-glucuronidase and the GUS colorimetric dye used to visualize beta-glucuronidase activity in plant tissue. It should be clear that the applicability of the present invention is not restricted to the nucleic acid represented by SEQ ID NO: 152, nor is the applicability of the invention restricted to the expression of a nucleic acid encoding an AT-hook domain and a DUF296 domain when directed by a prolamine promoter. Examples of other specific endosperm promoters that may also be used to carry out the methods of the invention are shown in Table 6 in the "Definitions" section.

Optionally, one or more terminator sequences may also be used in the construction introduced into a plant. The term "terminator" is defined in the "Definitions" section.

The genetic constructs of the invention may additionally include an origin of replication sequence that is required for maintenance and / or replication in a specific cell type. An example is when a genetic construct is required to be maintained in a bacterial cell as an episomal genetic element (e.g., plasmid or cosmid molecule). Preferred sources of replication include, but are not limited to, fl-ori and colE1.

The genetic construct may optionally comprise a selectable marker gene as defined herein. In a preferred embodiment, a gene construct is provided comprising:

(i) A nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain;

(ii) A prolamine promoter capable of preferentially directing nucleic acid sequence expression of (i) in endosperm tissue of a monocotyledonous plant; and optionally (iii) A transcription termination sequence.

The present invention also encompasses monocotyledonous plants obtainable by the methods according to the present invention. The present invention therefore provides monocotyledonous plants, parts thereof (including plant cells) obtainable by the methods according to the present invention, which plants or parts thereof comprise a transgene encoding a polypeptide comprising an AT-hook domain and a DUF296 domain operably linked to a specific endosperm promoter, preferably to a prolamine promoter.

The invention also provides a method for producing transgenic monocotyledonous plants having increased seed yield over suitable control plants, comprising introducing and expressing into a monocotyledonous plant a nucleic acid encoding a polypeptide comprising an AT-hook domain and a domain. DUF296, characterized in that said expression is preferably increased in endosperm tissue of a monocotyledonous plant.

More specifically, the present invention provides a method for producing transgenic monocotyledonous plants having increased seed production which method comprises:

(i) preferably introducing and increasing endosperm tissue expression of a monocotyledonous plant of a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain; and

(ii) cultivate the plant cell under conditions promoting plant growth and development.

Nucleic acid can be introduced directly into a plant cell of a monocot plant or in the plant itself (including introduction into a tissue, organ or any other part of a plant). According to a preferred feature of the present invention, the nucleic acid is preferably introduced into a plant by transformation.

The term "transformation" is defined in the "Definitions" section in this place.

The present invention clearly extends to any plant cell or plant produced by any of the methods described herein, and to all plant parts and seedlings thereof. The present invention further extends to encompass the progeny of a transformed or transfected primary cell, tissue, organ or whole plant that has been produced by any of the methods mentioned above, the sole requirement being that the progeny exhibit the same (s) genotypic and / or phenotypic trait (s) than those produced by the ancestor in the methods according to the invention.

The invention also includes host cells containing a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain operably linked to a specific endosperm promoter. Preferred host cells according to the invention are monocotyledonous plant cells.

The invention also extends to harvestable parts of a monocotyledonous plant such as, but not limited to seeds, leaves, fruits, flowers, stems, rhizomes, tubers and bulbs. The invention furthermore relates to products derived from, preferably directly derived from, a harvestable part of such a plant, such as dried pellets or powders, oil, fat and fatty acids, starch or proteins.

The present invention also encompasses use of a polypeptide-encoding nucleic acid comprising an AT-hook domain and a DUF296 domain to increase seed production of a monocotyledonous plant using the methods of the invention.

Detailed Description DOF Transcription Factors

The term "DOF transcription factor polypeptide" as defined herein refers to any polypeptide comprising feature (i) as follows, and additionally or feature (ii) or (iii) as follows:

(i) in ascending order of preference at least 60%, 65%, 70%, 75%, 80%, 85%, 90% or 95% sequence identity or with the DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and

(ii) in ascending order of preference at least 70%, 75%, 80%, 85%, 90% or 95% sequence identity with the DOF domain represented by SEQ ID NO: 200; or

(iii) Reason I: KALKKPDKILP (SEQ ID NO: 229) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or

Reason II: DDPGDCLFGKTIPF (SEQ ID NO: 230) with no change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position.

Additionally, polypeptides comprising feature (i) and feature (iii) above may comprise any, any two or all of the following motifs:

- Reason III: SPTLGKHSRDE (SEQ ID NO: 231) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or

- Reason IV: LQANPAALSRSQNFQE (SEQ ID NO: 232) without any change; or with one or more conservative changes in

any position; or with one, two or three non-conservative change (s) at any position; and / or

- Reason V: KGEGCLWVPKTLRID DPDEAAKSSIWT TLGIK (SEQ ID NO: 233) without any change; or with one or more conservative changes in any position; or with one, two, three, four or five non-conservative change (s) in any position.

A preferred polypeptide comprising feature (i) and feature (iii) above comprises both Reason I and II.

In addition, DOF transcription factor polypeptides (at least in their native form) typically have DNA binding activity and have an activation domain. The presence of a domain of activation and DNA binding activity can easily be determined by one of ordinary skill in the art using routine techniques and procedures.

SEQ ID NO: 199 (encoded by SEQ ID NO: 198) is an example of a DOF transcription factor polypeptide comprising characteristics (i) and (ii) as defined above, ie at least 60% sequence identity or with DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and at least 70% sequence identity to the DOF domain represented by SEQ ID NO: 200. Additional examples of DOF transcription factor polypeptides comprising characteristics (i) and (ii) as defined above are given in SEQ ID NO: 202 (encoded by SEQ ID NO: 201), SEQ ID NO: 204 (encoded by SEQ ID NO: 203), SEQ ID NO: 206 (encoded by SEQ ID NO: 205), SEQ ID NO: 208 (encoded by SEQ ID NO: 207), SEQ ID NO: 210 (encoded by SEQ ID NO: 209), SEQ ID NO: 212 (encoded by SEQ ID NO: 211), SEQ ID NO: 214 (encoded by SEQ ID NO: 213), SEQ ID NO: 216 (encoded by SEQ ID NO: 215), SEQ ID NO: 218 (encoded by SEQ ID NO: 217), SEQ ID NO: 220 (encoded by SEQ ID NO: 219), SEQ ID NO: 222 (encoded by SEQ ID NO: 221).

SEQ ID NO: 227 (encoded by SEQ ID NO: 226) is an example of a DOF transcription factor polypeptide comprising characteristics (i) and (iii) as defined above, ie at least 60% sequence identity or with DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and Reason I and / or Reason II as defined above. Additional examples of DOF transcription factor polypeptides comprising features (i) and (iii) as defined above are given in SEQ ID NO: 235 (encoded by SEQ ID NO: 234), SEQ ID NO: 237 (encoded by SEQ ID NO : 236), SEQ ID NO: 239 (encoded by SEQ ID NO: 238), SEQ ID NO: 241 (encoded by SEQ ID NO: 240), SEQ ID NO: 243 (encoded by SEQ ID NO: 242), SEQ ID NO: 245 (encoded by SEQ ID NO: 244), SEQ ID NO: 247 (encoded by SEQ ID NO: 246), SEQ ID NO: 249 (encoded by SEQ ID NO: 248), SEQ ID NO: 251 ( encoded by SEQ ID NO: 250), SEQ ID NO: 253 (encoded by SEQ ID NO: 252), SEQ ID NO: 255 (encoded by SEQ ID NO: 254).

Additional examples represented by SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO : 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 222 are examples of "homologues" of a DOF transcription factor polypeptide represented by SEQ ID NO: 199.

Additional examples represented by SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 245, SEQ ID NO: 247, SEQ ID NO : 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255 are examples of "homologues" of a DOF transcription factor polypeptide represented by SEQ ID NO: 227.

"Protein homologues" are as defined here in the "Definitions" section.

The DOF or homologue transcription factor polypeptide thereof may be a derivative. "Derivatives" are defined in the "Definitions" section here.

The various structural domains in a DOF transcription factor protein, such as the DOF domain, can be identified using specialized databases eg SMART (Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857- 5864; Letunic et al. (2002) Nucleic Acids Res 30, 242-244; http://smart.embl-heidelberg.de/), InterPro (Mulder et al. (2003) Nucl Acids Res. 31, 315-318; http://www.ebi.ac.uk/interpro/), Prosite (Bucher and Bairoch (1994), A generalized profile syntax for biomolecular sequence motifs and its funetion in automatic sequence interpretation. (In) ISMB- 94; Proceedings 2nd International Conference on Intelligent Systems for Molecular Biology Altman R., Brutlag D., Karp P., Lathrop R., Searls D., Eds., Pp53-61, AAAIPress, Menlo Park; Hulo et al. Nucl. Acids Res. 32: D134-D137, (2004), http://www.expasy.org/prosite/) or Pfam (Bateman et al., Nucleic Acids Research 30 (1): 276-280 (2002 ), http://www.sanger.ac.uk/Software/Pfam/).

Examples of nucleic acids encoding DOF transcription factor polypeptides (and homologues thereof) include those represented by any of: SEQ ID NO: 198, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 217, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 221, SEQ ID NO: 226, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 244, SEQ ID NO: 246, SEQ ID NO: 248, SEQ ID NO: 250, SEQ ID NO: 252 and SEQ ID NO: 254. Nucleic acid variants encoding DOF transcription factor polypeptides may be suitable for use in the methods of the invention. Suitable variants include nucleic acid moieties encoding DOF transcription factor polypeptides and / or nucleic acids capable of hybridizing to nucleic acids / genes encoding DOF transcription factor polypeptides. Additional variants include splice variants and allelic nucleic acid variants encoding DOF transcription factor polypeptides (and homologs thereof).

The term "portion" as defined herein refers to a piece of DNA encoding a polypeptide comprising feature (i) as follows, and additionally or feature (ii) or (iii) as follows:

(i) in ascending order of preference at least 60%, 65%, 70%, 75%, 80%, 85%, 90% or 95% sequence identity or with the DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and

(ii) in ascending order of preference at least 70%, 75%, 80%, 85%, 90% or 95% sequence identity with the DOF domain represented by SEQ ID NO: 200; or

(iii) Reason I: KALKKPDKILP (SEQ ID NO: 229) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or

Reason II: DDPGIKLFGKTIPF (SEQ ID NO: 230) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position.

Additionally feature (iii) above may comprise any, any two or all three of the following reasons:

- Reason III: SPTLGKHSRDE (SEQ ID NO: 231) without any change; or one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or

- Reason IV: LQANPAALSRSQNFQE (SEQ ID NO: 232) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or

- Reason V: KGEGCLWVPKTLRIDDPDE AAKSSIWTTL GIK (SEQ ID NO: 233) without any change; or with one or more conservative changes in any position; or with one, two, three, four or five non-conservative change (s) in any position.

A portion may be prepared, for example, by making one or more deletions to a nucleic acid encoding a DOF transcription factor polypeptide. The portions may be used in isolation or they may be fused to other coding (or non-coding) sequences to, for example, produce a protein that combines various activities.

When fused to other coding sequences, the resulting translation produced polypeptide may be larger than predicted for the DOF transcription factor portion.

Nucleic acid moieties encoding DOF transcription factor polypeptides comprising features (i) and (ii) as defined above are preferably portions of a nucleic acid as represented by any of: SEQ ID NO: 198, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO : 219 and SEQ ID NO: 221.

Nucleic acid moieties encoding DOF transcription factor polypeptides comprising features (i) and (iii) as defined above are preferably portions of a nucleic acid as represented by any of: SEQ ID NO: 226, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 244, SEQ ID NO: 246, SEQ ID NO: 248, SEQ ID NO: 250, SEQ ID NO : 252 and SEQ ID NO: 254.

Another variant of a DOF transcription factor gene / nucleic acid is a nucleic acid capable of hybridizing under reduced stringency conditions, preferably under stringent conditions, with a nucleic acid / DOF transcription factor gene as defined above, whose hybridizing sequence encodes a polypeptide comprising feature (i) as follows and additionally or feature (ii) or (iii) as follows:

(i) in ascending order of preference at least 60%, 65%, 70%, 75%, 80%, 85%, 90% or 95% sequence identity or with the DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and

(ii) in ascending order of preference at least 70%, 75%, 80%, 85%, 90% or 95% sequence identity with the DOF domain represented by SEQ ID NO: 200; or

(iii) Reason I: KALKKPDKILP (SEQ ID NO: 229) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or

Reason II: DDPGIKLFGKTIPF (SEQ ID NO: 230) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position.

Additionally feature (iii) above may comprise any, any two or all three of the following reasons:

- Reason III: SPTLGKHSRDE (SEQ ID NO: 231) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or

- Reason IV: LQANPAALSRSQNFQE (SEQ ID NO: 232) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or

- Reason V: KGEGCLWVPKTLRIDDPDEAAK SSIWTTL GIK (SEQ ID NO: 233) without any change; or with one or more conservative changes in any position; or with one, two, three, four or five non-conservative change (s) in any position.

Preferably, the hybridizing sequence encoding DOF transcription factor polypeptides comprising characteristics (i) and (ii) as defined above is a sequence capable of hybridizing to a nucleic acid as represented by any of: SEQ ID NO: 201, SEQ ID NO : 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219 and SEQ ID NO: 221.

Preferably, the hybridizing sequence encoding DOF transcription factor polypeptides comprising characteristics (i) and (iii) as defined above is a sequence capable of hybridizing to a nucleic acid as represented by any of: SEQ ID NO: 234, SEQ ID NO : 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 244, SEQ ID NO: 246, SEQ ID NO: 248, SEQ ID NO: 250, SEQ ID NO: 252 and SEQ ID NO: 254.

The term "hybridization" is as defined here in the "Definitions" section.

The DOF transcription factor polypeptide may be encoded by an alternative binding variant. The term "alternate union variant" is as defined in the "Definitions" section here.

Preferred splice variants are nucleic acid splice variants encoding a polypeptide comprising feature (i) as follows and additionally or feature (ii) or (iii) as follows: (i) in ascending order preferably at least 60%, 65% , 70%, 75%, 80%, 85%, 90% or 95% of sequence identity or with the DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and

(ii) in ascending order of preference at least 70%, 75%, 80%, 85%, 90% or 95% sequence identity with the DOF domain represented by SEQID NO: 200; or

(iii) Reason I: KALKKPDKILP (SEQ ID NO: 229) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or

Reason II: DDPGDCLFGKTIPF (SEQ ID NO: 230) with no change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position.

Preferred nucleic acid splice variants encoding DOF transcription factor polypeptides comprising features (i) and

(ii) as defined above are splice variants of a nucleic acid as represented by any of: SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209 , SEQ ID NO: 211, SEQ ID

NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219 and SEQ ID NO: 221.

Preferred nucleic acid splice variants encoding DOF transcription factor polypeptides comprising features (i) and

(iii) as defined above are preferably splice variants of a nucleic acid as represented by any of: SEQ ID NO: 234, SEQ ID

NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 244, SEQ ID NO: 246, SEQ ID NO: 248, SEQ ID NO: 250, SEQ ID NO: 252 and SEQ ID NO: 254.

The DOF transcription factor polypeptide can also be encoded by an allelic variant, which are also defined in the "Definitions" section here.

Preferred allelic variants are allelic variants of the nucleic acid encoding a polypeptide comprising feature (i) as follows and additionally or feature (ii) or (iii) as follows:

(i) in ascending order of preference at least 60%, 65%, 70%, 75%, 80%, 85%, 90% or 95% sequence identity or with the DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and

(ii) in ascending order of preference at least 70%, 75%, 80%, 85%, 90% or 95% sequence identity with the DOF domain represented by SEQ ID NO: 200; or

(iii) Reason I: KALKKPDKILP (SEQ ID NO: 229) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or

Reason II: DDPGIKLFGKTIPF (SEQ ID NO: 230) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position.

Preferred allelic variants of nucleic acids encoding DOF transcription factor polypeptides comprising features (i) and (ii) as defined above are splice variants of a nucleic acid as represented by any of: SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219 and SEQ ID NO: 221.

Preferred allelic variants of nucleic acids encoding DOF transcription factor polypeptides comprising features (i) and (iii) as defined above are preferably portions of a nucleic acid as represented by any of: SEQ ID NO: 234, SEQ ID NO: 236 , SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 244, SEQ ID NO: 246, SEQ ID NO: 248, SEQ ID NO: 250, SEQ ID NO: 252, and SEQ ID NO: 254.

Additional variants of nucleic acids encoding DOF transcription factor polypeptides as defined above may be generated using, for example, site-directed mutagenesis as defined in the "Definitions" section herein.

Direct evolution (or gene shuffling) can also be used to generate nucleic acid variants encoding DOF transcription factor polypeptides. See section "definitions".

DOF transcription factor polypeptides are plant specific. Nucleic acids encoding them may be derived from any natural or artificial source. The nucleic acid or variant thereof may be modified from its native form in genomic composition and / or environment by deliberate human manipulation. Preferably the DOF transcription factor nucleic acid or variant thereof is from a dicotyledonous plant, still preferably from the Brassicaceae family, more preferably the nucleic acid is from Arabidopsis thaliana.

The expression of a nucleic acid encoding a DOF transcription factor polypeptide can be increased by introducing a genetic modification (preferably at the locus of a DOF transcription factor gene). The locus of a gene as defined herein is adopted to mean a genomic region, which includes the gene of interest and 10 KB before or after the coding region.

Genetic modification may be introduced, for example, by any (or more) of the following methods: T-DNA activation, TILLING and homologous recombination or by introducing and expressing into a plant a nucleic acid encoding a DOF transcription factor polypeptide. T-DNA5 TILLING activation and homologous recombination methods are as defined in the "Definitions" section here. Following introduction of genetic modification, there is an additional step of selecting for increased expression of a nucleic acid encoding a DOF5 transcription factor polypeptide whose increased expression generates plants having increased yield.

T-DNA activation and TILLING are examples of technologies that enable the generation of new alleles and DOF transcription factor variants.

A preferred method for introducing a genetic modification (which in this case need not be at the locus of a DOF transcription factor gene) is to introduce and express into a plant a nucleic acid encoding a DOF transcription factor polypeptide as defined above. The nucleic acid to be introduced into a plant may be a full length nucleic acid or may be a hybridizing portion or sequence or other nucleic acid variant as defined above.

The methods of the invention employ increased expression of a nucleic acid encoding a DOF transcription factor polypeptide. Methods for enhancing gene expression or gene products are well documented in the art and include, for example, overexpression directed by appropriate promoters, the use of transcriptional enhancers or translation enhancers. Isolated nucleic acids serving as promoter or enhancer elements may be introduced at an appropriate (typically prior) position in a nonheterologous form of a polynucleotide in order to enhance expression of a nucleic acid encoding a DOF transcription factor polypeptide. For example, endogenous promoters may be altered in vivo by mutation, deletion, and / or substitution (see, Kmiec, U.S. Patent No. 5,565,350; Zarling et al., PCT / US93 / 03868), or isolated promoters. be introduced into a plant cell at the appropriate orientation and distance of a gene of the present invention to control gene expression.

If polypeptide expression is desired, it is generally desirable to include a polyadenylation region at the 3 'end of a polynucleotide coding region. The polyadenylation region may be derived from the natural gene, a variety of other plant genes, or T-DNA. The 3 'end sequence to be added may be derived, for example, from the nopaline synthase or octopine synthase genes, or alternatively from another plant gene, or less preferably from any other eukaryotic gene.

An intronic sequence can also be added to the untranslated 5 'region or coding sequence of the partial coding sequence to increase the amount of mature message that accumulates in the cytosol. Inclusion of a processable intron in the transcription unit in both plant and animal constructs has been shown to increase gene expression at both mRNA and protein levels by up to 1000-fold (Buchman and Berg (1988) Mol. Cell biol. 8: 4395-4405; Callis et (1987) Genes Dev 1: 1183-1200). Such intronic stimulation of gene expression is typically greater when located near the 5 'end of the transcription unit. Using the introns of Adh1-S 1, 2, and 6 intron maize, the Bronze-I intron are known in the art. For general information see: The Maize Handbook, Chapter 116, Freeling and Walbot, Eds., Springer, N.Y. (1994).

The invention also provides genetic constructs and vectors to facilitate introduction and / or expression of nucleotide sequences useful in the methods according to the invention.

Therefore, a gene construct is provided comprising:

(i) A nucleic acid or variant thereof encoding a DOF transcription factor polypeptide as defined above;

(ii) One or more control sequences capable of directing expression of the nucleic acid sequence of (i); and optionally

(iii) A transcription termination sequence.

Constructs useful in the methods according to the present invention may be constructed using recombinant DNA technology well known to those skilled in the art. Gene constructs may be inserted into commercially available vectors suitable for plant transformation and suitable for expression of the gene of interest in transformed cells. The invention therefore provides use of a gene construct as defined above in the methods of the invention.

Plants are transformed with a vector comprising the sequence of interest (i.e., a nucleic acid encoding a DOF transcription factor polypeptide). The sequence of interest is operably linked to one or more control sequences (at least one promoter). The terms "regulatory element", "control sequence" and "promoter" are all used interchangeably in this place and are defined in the "Definitions" section in this place.

Advantageously, any type of promoter, whether natural or synthetic, may be used to direct expression of the nucleic acid sequence.

According to a preferred feature of the invention, the transcription factor DOF nucleic acid or variant thereof is operably linked to a constitutive promoter as defined in the "Definitions" section herein. The constitutive promoter is preferably a G0S2 promoter, more preferably the constitutive promoter is a rice G0S2 promoter, yet preferably the constitutive promoter is represented by a nucleic acid sequence substantially similar to SEQ ID NO: 225, most preferably the constitutive promoter is as represented by SEQ ID NO: 225. It is preferred to use a constitutive promoter to direct expression of a nucleic acid encoding a DOF transcription factor polypeptide comprising characteristics (i) and (ii) as defined above, ie at least 60%. sequence identity or DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and at least 70% sequence identity with the DOF domain represented by SEQ ID NO: 200.

It should be clear that the applicability of the present invention is not restricted to the DOF transcription factor nucleic acid represented by SEQ ID NO: 198, nor is the applicability of the invention restricted to the expression of a DOF transcription factor nucleic acid when directed by a GOS2 promoter. Examples of other constitutive promoters that may also be used to carry out the methods of the invention are shown in Table 3 in the "Definitions" section herein.

According to another preferred feature of the invention, the nucleic acid encoding a DOF transcription factor polypeptide is operably linked to a specific seed promoter, ie a promoter that is predominantly expressed in seed tissue, but which may have residual expression in any other. place in the plant due to permeable promoter expression. Even more preferably, the specific seed promoter is isolated from a gene encoding a seed storage protein, especially a specific endosperm promoter. More preferably the specific endosperm promoter is isolated from a prolamine gene, such as a rice RP6 prolamine promoter (Wen et al., (1993) Plant physiol 101 (3): 1115-6) as represented by SEQ ID NO: 258, or a similar strength promoter and / or a promoter with a similar expression pattern as the rice prolamine promoter. Similar force and / or similar expression pattern can be analyzed, for example, by coupling promoters to a reporter gene and by checking reporter gene function in plant tissues. A well-known reporter gene is beta-glucuronidase and the GUS colorimetric dye used to visualize beta-glucuronidase activity in plant tissue. The prolamine promoter shows strong endosperm expression with meristem permeability, more specifically the bud meristem and / or meristem discrimination center.

Preferred according to the invention is the use of a specific seed promoter, especially a specific endosperm promoter, to direct expression of a nucleic acid encoding a DOF transcription factor polypeptide comprising features (i) and (iii) as defined above, ie at least 60% sequence identity or with the DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and Reason I and / or Reason II.

It should be clear that the applicability of the present invention is not restricted to the DOF transcription factor nucleic acid represented by SEQ ID NO: 226, nor is the applicability of the invention restricted to the expression of a DOF transcription factor nucleic acid when directed by a prolamine promoter.

Examples of specific seed promoters are presented in Table 7 in the "Definitions" section herein, whose promoters or derivatives thereof are useful for carrying out the methods of the present invention.

Optionally, one or more terminator sequences may also be used in the construction introduced into a plant. The term "terminator" is as defined in the "Definitions" section here.

The genetic constructs of the invention may additionally include an origin of replication sequence that is required for maintenance and / or replication in a specific cell type. An example is when a genetic construct is required to be maintained in a bacterial cell as an episomal genetic element (e.g., plasmid or cosmid molecule). Preferred sources of replication include, but are not limited to, fl-ori and colEl.

The genetic construct may optionally comprise a selectable marker gene as defined herein in the "Definitions" section.

The present invention also encompasses plants obtainable by the methods according to the present invention. The present invention therefore provides plants, plant parts or plant cells thereof obtainable by the method according to the present invention, which plants or parts or cells thereof comprise a transgene nucleic acid (or variant thereof as defined above) encoding a transcription factor polypeptide. DOF.

The invention also provides a method for producing transgenic plants having increased production over suitable control plants, comprising introducing and expressing into a plant a nucleic acid or variant thereof encoding a DOF transcription factor polypeptide.

More specifically, the present invention provides a method for producing transgenic plants having increased yield which method comprises:

(i) introducing and expressing into a plant, plant part or plant cell a nucleic acid or variant thereof encoding a DOF transcription factor polypeptide; and

(ii) cultivate the plant cell under conditions promoting plant growth and development.

Nucleic acid can be introduced directly into a plant cell or into the plant itself (including introduction into a tissue, organ or any other part of a plant). According to a preferred feature of the present invention, the nucleic acid is preferably introduced into a plant by transformation.

The term "transformation" is as defined here in the "Definitions" section.

The present invention clearly extends to any plant cell or plant produced by any of the methods described herein, and to all plant parts and seedlings thereof. The present invention further extends to encompass the progeny of a transformed or transfected primary cell, tissue, organ or whole plant that has been produced by any of the methods mentioned above, the sole requirement being that the progeny exhibit the same (s) genotypic and / or phenotypic trait (s) than those produced by the ancestor in the methods according to the invention.

The invention also includes host cells containing an isolated nucleic acid or variant thereof encoding a DOF transcription factor polypeptide. Preferred host cells according to the invention are plant cells.

The invention also extends to harvestable parts of a plant such as, but not limited to seeds, leaves, fruits, flowers, stems, rhizomes, tubers and bulbs. The invention further relates to products derived, preferably directly derived, from a crop-fit part of such a plant, such as dried pellets or powders, oil, fat and fatty acids, starch or proteins.

The present invention also encompasses use of nucleic acids or variants thereof encoding DOF transcription factor polypeptides and use of DOF transcription factor polypeptides to increase plant yield as defined above in the methods of the invention.

Nucleic acids or variants thereof encoding DOF transcription factor polypeptides, or DOF transcription factor polypeptides, may find use in breeding programs in which a DNA marker is identified that can be genetically linked to a DOF transcription factor gene or variant of this. Nucleic acids / genes or variants thereof, or DOF transcription factor polypeptides may be used to define a molecular marker. This DNA or protein marker can then be used in breeding programs to select plants having increased yield as defined above in the methods of the invention.

Allelic variants of a nucleic acid / DOF transcription factor gene may also find use in marker-assisted breeding programs. Such breeding programs sometimes require introduction of allelic variation by mutagenic treatment of plants, using for example EMS mutagenesis; alternatively, the program may start with a collection of allelic variants of the so-called "natural" origin caused unintentionally. Identification of allelic variants then occurs, for example, by PCR. This is followed by a step for selecting superior allelic variants of the sequence in question and generating increased production. Selection is typically performed by monitoring plant growth performance containing different allelic variants of the sequence in question. Growth performance can be monitored in a greenhouse or in the field. Additional optional steps include crossing plants in which the upper allelic variant has been identified with another plant. This can be used, for example, to make a combination of interesting phenotypic characteristics.

A nucleic acid or variant thereof encoding a DOF transcription factor polypeptide may also be used as probes to genetically and physically map the genes of which they are a part of, and as markers for traits linked to these genes. Such information may be useful in plant breeding in order to develop strains with desired phenotypes. Such use of DOF transcription factor nucleic acids or variants thereof requires only a nucleic acid sequence of at least 15 nucleotides in length. DOF transcription factor nucleic acids or variants thereof can be used as restriction fragment length polymorphism (RFLP) markers. Southern blots (Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning, A Laboratory Manual) of restriction digested plant genomic DNA can be probed with the DOF transcription factor nucleic acids or variants thereof. The resulting banding patterns can then be subjected to genetic analysis using computer programs such as MapMaker (Lander et al. (1987) Genomics 1: 174-181) in order to construct a genetic map. In addition, nucleic acids can be used to probe Southern blots containing restriction endonuclease-treated genomic DNAs from a set of individuals representing ancestor and progeny of a defined genetic crossover. Segregation of DNA polymorphisms is observed and used to calculate the position of the DOF transcription factor nucleic acid or variant on the genetic map previously obtained using this population (Botstein et al. (1980) Am. J. Hum. Genet. 32: 314-331).

The production and use of plant gene derived probes for use in genetic mapping are described in Bematzky and Tanksley (1986) Plant Mol. Biol. Reporter 4: 37-41. Numerous publications describe genetic mapping of specific cDNA clones using the methodology described above or variations thereof. For example, F2 crossover populations, inbreeding populations, randomly crossed populations, nearby isogenic strains, and other sets of individuals may be used for mapping. Such methodologies are well known to those skilled in the art.

Nucleic acid probes may also be used for physical mapping (ie, location of sequences in physical maps; see Hoheisel et al. In: Non-mammalian Genomic Analysis: A Practical Guide, Academic press 1996, pp. 319-346, and references cited there).

In another embodiment, nucleic acid probes may be used in direct mapping by fluorescence in situ hybridization (FISH) (Trask (1991) Trends Genet. 7: 149-154). Although current FISH mapping methods favor the use of large clones (several kb to several hundred kb; see Laan et al. (1995) Genome Res. 5: 13-20), sensitivity improvements may allow FISH mapping performance using probes. smaller ones.

A variety of nucleic acid amplification based methods for genetic and physical mapping can be performed using nucleic acids. Examples include allele-specific amplification (Kazazian (1989) J. Lab. Clin. Med. 11: 95-96), PCR amplified fragment polymorphism (CAPS; Sheffield et al. (1993) Genomics 16: 325-332), allele binding specific (Landegren et al (1988) Science 241: 1077-1080), nucleotide extension reactions (Sokolov (1990) Nucleic Acid Res. 18: 3671), Radiation Hybrid Mapping (Walter et al. (1997) Nat. Genet. 7: 22-28) and Appropriate Mapping (Dear and Cook (1989) Nucleic Acid Res. 17: 6795-6807). For these methods, the nucleic acid sequence is used to design and produce primer pairs for use in the amplification reaction or primer extension reactions. The design of such initiators is well known to those skilled in the art. In methods employing PCR-based genetic mapping, it may be necessary to identify DNA sequence differences between the mapping ancestors in the region corresponding to the immediate sequence of nucleic acids. This, however, is generally not required for mapping methods.

The methods according to the present invention result in plants having increased yield as described above. This increased yield can also be combined with other economically advantageous features, such as additional features that stimulate yield, tolerance to other abiotic and biotic stresses, features modifying various architectural attributes and / or biochemical and / or physiological attributes.

Detailed Description CKI Reference herein to a preferred "reduction" in expression of an endogenous CKI gene in endosperm tissue of a plant is adopted to mean a substantial reduction or deletion of expression of an endogenous CKI gene (in endosperm tissue) in of endogenous CKI gene expression levels found in endosperm tissue of wild type plants. This substantial reduction or elimination of endogenous CKI gene expression may result in reduced or substantially eliminated CKI protein levels and / or activity in endosperm tissue of a plant.

Reference herein to an "endogenous" CKI gene not only refers to CKI genes as found in a plant in its natural form (i.e. without any human intervention), but also refers to isolated CKI genes subsequently introduced into a plant. For example, a transgenic plant containing a CKI transgene may encounter a substantial reduction or deletion of the CKI transgene and / or a substantial reduction or deletion of an endogenous CKI gene (in endosperm tissue).

This reduction (or substantial elimination) of endogenous CKI gene expression can be achieved using any one or more of several well known gene silencing methods. "Gene silencing" or "decreased" expression as used herein refers to a substantial reduction or elimination of CKI gene expression and / or CKI polypeptide levels and / or CKL polypeptide activity.

One such method for substantially reducing or eliminating endogenous CKI gene expression is RNA-mediated downregulation of gene expression (RNA silencing). Silencing in this case is triggered in a plant by a double-stranded RNA (dsRNA) molecule that is substantially homologous to a target CKI gene. This dsRNA is further processed by the plant at about 21 to about 26 nucleotides called low interference RNAs (siRNAs). These siRNAs are incorporated into a (RISC) induced silencing complex that cleaves the mRNA of a target CKI gene, thereby reducing or substantially eliminating the number of CKI mRNAs to be translated into a CKL protein.

An example of an RNA silencing method involves introducing coding sequences or parts of these in a sense orientation into a plant. "Sense orientation" refers to DNA that is homologous to an mRNA transcript thereof. Therefore, at least one additional copy (complete or in part) of a CKI gene already present in the host plant must be introduced into a plant. The additional gene, or part of it, will silence an endogenous CKI gene, generating a phenomenon known as co-suppression. Reduction of CKI gene expression will be more pronounced if several additional copies are introduced into the plant as there is a positive correlation between high transcript levels and co-suppression triggering.

Another example of an RNA silencing method involves the use of antisense CKI nucleic acid sequences. An "antisense" nucleic acid comprises a nucleotide sequence that is complementary to a "sense" nucleic acid encoding a protein, e.g., complementary to the coding strand of a double stranded cDNA molecule or complementary to an mRNA sequence. Accordingly, an antisense nucleic acid may be bridged to a sense nucleic acid. Antisense nucleic acid may be complementary to an entire CKI coding tape or to a portion thereof. The antisense nucleic acid molecule may be antisense to a "coding region" or antisense to a "non-coding region" of the coding strand of a CKL coding nucleotide sequence. refers to the region of the nucleotide sequence comprising codons that are translated into amino acid residues. The term "non-coding region" refers to 5 'and 3' sequences flanking the coding region that are not translated into amino acids (i.e., also referred to as 5 'and 3' untranslated regions).

Antisense nucleic acids can be designed according to the Watson and Crick base pairing rules. The antisense nucleic acid molecule may be complementary to the entire CKI mRNA coding region, but is preferably an oligonucleotide that is antisense to only a portion of the CKI mRNA coding or non-coding region. For example, the antisense oligonucleotide may be complementary to the region around the CKI mRNA translation initiation site. The length of a suitable antisense oligonucleotide should be known in the art and may start from about 20 nucleotides in length or less. An antisense nucleic acid of the invention may be constructed using chemical synthesis and enzymatic binding reactions using procedures known in the art. For example, an antisense nucleic acid (eg, an antisense oligonucleotide) may be chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between acids. antisense and sense nucleicides, eg, phosphorothioate derivatives and substituted acridine nucleotides may be used. Examples of modified nucleotides that can be used to generate antisense nucleic acid are well known in the art.

Other known nucleotide modifications include methylation, cyclization and caps and replacement of one or more of the naturally occurring nucleotides with an analog such as inosine. Other nucleotide modifications are well known to one of ordinary skill in the art. Alternatively, antisense nucleic acid may be biologically produced using an expression vector in which a nucleic acid has been subcloned in an antisense orientation (ie, RNA transcribed from the inserted nucleic acid will be from an antisense orientation to a nucleic acid of interest, described later in the following subsection). Preferably, production of antisense nucleic acids in plants occurs by a stably integrated transgene comprising an operative promoter for preferential expression in endosperm tissue plants, an antisense oligonucleotide, and a terminator.

A preferred method for substantially reducing or eliminating endogenous CKI gene expression through RNA silencing is by using an expression vector in which a CKI gene or fragment thereof has been cloned as an inverted repeat (in part or completely), separated by a spacer (Non-coding DNA). After transcription of the inverted repeat, a chimeric CKI RNA with a self-complementing structure is formed (partial or complete). This double stranded RNA structure is referred to as the stapled RNA structure (hpRNA). HpRNA is processed by the plant into siRNAs that are incorporated into a RISC. RISC subsequently cleaves mRNA from a target CKI gene, thereby reducing or substantially eliminating the number of CKI mRNAs to be translated into a CKL protein. See for example, Grierson et al. (1998) WO 98/53083; Waterhouse et al (1999) WO 99/53050).

Nucleic acid molecules used for silencing in the methods of the invention (whether introduced into a plant or generated in situ) hybridize to or bind to cellular mRNA and / or genomic DNA encoding a CKI protein to thereby inhibit protein expression, eg. inhibiting transcription and / or translation. Hybridization may be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule that binds to DNA duplexes, through specific interactions in the main double strand slit. Antisense nucleic acid molecules can be introduced into a plant by transformation or direct injection into a specific tissue site. Alternatively, antisense nucleic acid molecules may be modified to target selected cells and then systematically administered. For example, for systemic administration, antisense molecules may be modified so that they specifically bind to receptors or antigens expressed on a selected cell surface, eg, by binding antisense nucleic acid molecules to peptides or antibodies that bind. to cell surface receptors or antigens. Antisense nucleic acid molecules can also be released into cells using the vectors described herein.

In a further aspect, the antisense nucleic acid is an α-anomeric nucleic acid molecule. An α-anomeric nucleic acid molecule forms complementary RNA-specific double strand hybrids in which, unlike the usual b-units, strands run parallel to each other (Gaultier et al. (1987) Nucleic Aeids. Res. 15: 6625- 6641). The antisense nucleic acid molecule may also comprise a 2'-o-methylribonucleotide (Inoue et al. (1987) Nueleie Aeids Res. 15: 6131-6148) or a chimeric RNA-DNA analog (Inoue et al. ( 1987) FEBS Lett 215: 327-330).

In yet another embodiment, an antisense nucleic acid of the invention is a ribozyme. Ribozymes are ribonuclease activity catalytic RNA molecules that are capable of cleaving a single stranded nucleic acid, such as an mRNA, to which they have a complementary region. Thus, ribozymes (eg, hammerhead ribozymes (described in Haselhoff and Gerlach (1988) Nature 334: 585-591)) can be used to catalytically cleave CKI mRNA transcripts to thereby inhibit CKL mRNA translation. ribozyme having specificity for a CKI-encoding nucleic acid can be designed based on the nucleotide sequence of a CKL cDNA. For example, a derivative of a Tetrahymena L-19 LVS RNA can be constructed in which the nucleotide sequence of the active site is complementary. to the nucleotide sequence to be cleaved into a mRNA encoding CKL See 5 eg, Cech et al. U.S. Patent No. 4,987,071; and Cech et al. U.S. Patent No. 5,116,742. Alternatively, CKI mRNA can be used to select a catalytic RNA having ribonuclease specific activity from a set of RNA molecules. See, e.g., Bartel, D. and Szostak, J.W. (1993) Science 261: 1411-1418. The use of plant gene silencing ribozymes is known in the art (eg, Atkins et al. (1994) WO 94/00012; Lenne et al. (1995) WO 95/03404; Lutziger et al. (2000) WO 00 Prinsen et al (1997) WO 97/13865 and Scott et al (1997) WO 97/38116).

Gene silencing can also be achieved by insertion mutagenesis (eg, T-DNA insertion or transposon insertion) or by gene silencing strategies as described by, among others, Angell and Baulcombe 1998 (Amplicon VIGS WO 98/36083 ); Baulcombe (WO 99/15682).

Gene silencing can also occur if there is a mutation in the endogenous CKI gene and / or a mutation in an isolated CKI gene subsequently introduced into a plant. Substantial reduction or elimination of CKI expression may be caused by a non-functional CKI. CKI binds to both CDK and cyclins (Verkest et al., (2005) Plant Cell 17: 1723-1736). For example, mutation of the cyclin binding site within a CKI supports a CKI that can still bind to a CDK but cannot inhibit the active CDK-cyclin complex.

An additional approach to gene silencing is by directing complementary nucleotide sequences to the CKI regulatory region (e.g., the CKI promoter and / or enhancers) to form triple helix structures that prevent transcription of the CKI gene into target cells. See Helene, C. (1991) Anticancer Drug Des. 6 (6): 569-84; Helene, C. et al. (1992) Ann. N.Y. Aead. Know. 660: 21-36-, and Maher, LJ. (1992) Bioassays 14 (12): 807-15.

Examples of various methods for gene silencing (for the substantial reduction or elimination of endogenous CKI gene expression) are described above. The methods of the invention are based on preferential reduction of expression of an endogenous CKI gene in endosperm tissue of a plant. One of ordinary skill in the art should readily be able to adapt the abovementioned methods for silencing to achieve preferential gene silencing in endosperm tissue through the use of an appropriate promoter, for example.

It should be noted that the essence of the present invention resides in the advantageous and surprising results found by substantially reducing or eliminating endogenous CKI gene expression in endosperm tissue of a plant, and is not limited to any particular method for such substantial reduction or elimination of CKI. endogenous CKI gene expression. Other such methods will be known to the skilled man.

For optimal performance, gene silencing techniques used for the substantial reduction or elimination of endogenous CKI gene expression require the use of CKI nucleic acid sequences from monocotyledonous plants for transformation into monocotyledonous plants. Preferably, a CKI nucleic acid of any given plant species is introduced into that same species. For example, a rice CKI nucleic acid (be it a full length CKI sequence or a fragment) is transformed into a rice plant. CKI nucleic acid need not be introduced into the same plant variety.

Reference herein to a "CKI gene" or CKI nucleic acid "is adopted to mean a polymeric form of a deoxyribonucleotide or ribonucleotide polymer of any length, or double stranded or single stranded, or analogs thereof, having the following characteristics: essential characteristics of a natural ribonucleotide with which they can hybridize to nucleic acids in a manner similar to naturally occurring polynucleotides. A "CKI gene" or a CKI nucleic acid "refers to a sufficient length of substantially contiguous nucleotides of a gene encoding CKI to perform gene silencing; This can be as little as 20 or less nucleotides. A gene encoding a (functional) protein is not a requirement for the various methods discussed above for substantially reducing or eliminating expression of an endogenous CKI gene.

The methods of the invention may be performed using a sufficient length of substantially contiguous nucleotides of a CKI gene / nucleic acid, which may consist of 20 or fewer nucleotides, which may be from any part of the CKI gene / nucleic acid, such as 3 'end of the coding region that is well conserved within the CKI gene family.

CKI genes are well known in the art and useful in the methods of the invention are substantially contiguous nucleotides of any of the plant CKI genes / nucleic acids described in published International patent application WO 2005/007829 in the name of Monsanto Technology LLC and Published International Patent Applications, WO 02/28893 and WO 99/14331 in the name of CropDesign NV, whose CKI nucleotide genes / sequences are incorporated herein as if fully exposed.

Other CKI nucleic acid genes / sequences may also be used in the methods of the invention, and may be readily identified by one of ordinary skill in the art. CKI polypeptides may be identified by the presence of one or more of several well known characteristics (see below). Upon identification of a CKI polypeptide, one skilled in the art can readily derive, using routine techniques, the corresponding coding nucleic acid sequence and use a sufficient length of contiguous nucleotides thereof to perform any one or more of the silencing methods. genes described above (for the substantial reduction or elimination of expression of an endogenous CKI gene in the endosperm).

A distinguishing feature of a CKI polypeptide is a C-terminal region comprising from about 40 to about 55 highly conserved amino acids. As a guideline, polypeptides preferably comprising in ascending order at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62 %, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95% 96%, 97%, 98%, 99% C-terminal region identity of a CKI as represented by SEQ ID NO: 262 can be considered to be CKI homologs. One skilled in the art can readily derive the corresponding nucleic acid encoding such homologs, and use a sufficient length of contiguous nucleotides thereof to perform any one or more of the gene silencing methods described above (for the substantial reduction or elimination of expression of an endogenous CKI gene).

One skilled in the art will be aware of what is meant by a "C-terminus" of a protein; For purposes of this application, the C-terminal region of a CKI may be considered to be the second half (from N-terminal to C-terminal) of a full-length CKI polypeptide.

Homologs as defined above, ie polypeptides comprising at least 50% C-terminal region identity of a CKI as represented by SEQ ID NO: 262, can be readily identified using routine techniques well known in the art, such as by alignment. of sequences. Methods for sequence alignment for comparison are well known in the art, such methods include GAP, BESTFIT, BLAST, FASTA and TFASTA. GAP uses the algorithm of Needleman and Wunsch ((1970) J Mol Biol 48: 443-453) to find the alignment of two complete sequences that maximizes the number of identities and minimizes the number of gaps. The BLAST algorithm (Altschul et al. (1990) J Mol Biol 215: 403-10) calculates percent sequence identity and performs a statistical analysis of similarity between the two sequences. The computer application for performing BLAST analysis is publicly available through the National Center for Biotechnology Information. Homologous sequences can be readily identified using, for example, the ClustalW multiple sequence alignment algorithm (version 1.83) available at http://clustalw.genome.jp/sit-bin/nph-ClustalW, with standard paired alignment parameters. , and a percentage scoring method. Secondary manual editing can be performed to optimize alignment between retained subjects (see below), as should be apparent to one of ordinary skill in the art.

Plant CKI polypeptides can also be identified by the presence of certain conserved motifs (see Table 12 below). The presence of these conserved motifs can be identified using methods for sequence alignment for comparison as described above. In some instances, the default parameters may be adjusted to modify the search stringency. For example using BLAST, the threshold of statistical significance (called the "expected" value) for reporting identities against database sequences can be increased to show less stringent identities. In this way, practically exact short identities can be identified. Upon identification of a CKI polypeptide by the presence of these motifs, one skilled in the art can readily derive the corresponding nucleic acid encoding the polypeptide comprising the relevant motifs, and use a sufficient length of contiguous nucleotides thereof to perform any one or more of the methods of gene silencing described above (for the substantial reduction or elimination of expression of an endogenous CKI gene).

Typically, the presence of at least one of reasons 1 to 5 (for example reason 2 is particularly well maintained) should be sufficient to identify any query sequence as a CKI, however for increased certainty, the presence of at least Reasons 1, 2 and 3 is preferred. The consensus sequence provided is based on the sequences presented in Table 12 below. One skilled in the art should be well aware that the consensus sequence may vary somewhat if additional or different sequences were used for comparison.

Reason 1: FXXKYNFD (SEQ ID NO: 261), characterized in that X is any amino acid

Reason 2: [P / L] LXGRYEW (SEQ ID NO: 262), characterized in that X is any amino acid and [P / L] means that either a proline or a leucine appears in the position indicated.

Reason 3: EXE [D / E] FFXXXE (SEQ ID NO: 263), characterized in that X is any amino acid and [D / E] means that either an aspartate or a glutamate appears in the position indicated.

Reason 4: YXQLRSRR (SEQ ID NO: 264), characterized in that X is any amino acid

Reason 5: MGKY [M / I] [K / R] KX [K / R] (SEQ ID NO: 265), characterized in that X is any amino acid, [M / I] means that either a methionine or a isoleucine appear at the indicated position, and [K / R] means that either a lysine or arginine appear at the indicated position

Reason 6: SXGVRTRA (SEQ ID NO: 266), characterized in that X is any amino acid

Motifs 1, 2, and 3 are typically found in the carboxyl terminal region of plant CKI proteins. This region is believed to be involved in the interaction of CKIs with both CDKs and cyclins (Chen et al. (1996) MolCellBiol 16, 4673-4682, Matsuoka et al. (1995) Genes Dev. 9, 650-662, and Nakayama and Nakayama (1998) Bioessays 20, 1020-1029). Motifs 4, 5, and 6 are typically found in the amino terminal region of plant CKI proteins.

CKI proteins from monocotyledonous plants, particularly rice, are characterized by extensive α-helical stretches especially between motifs 5 and 6 and between motifs 6 and 4.

Table 12. Reasons conserved in vegetable CKI proteins. CKI1 through CKI7 denote Arabidopsis thaliana CKIs. Os: Oryza sativa, Zm: Zea mays, Sb: Sorghum bicolor

<table> table see original document page 175 </column> </row> <table> <table> table see original document page 176 </column> </row> <table>

In addition to the features mentioned above, a CKI protein may also comprise any or more of the following: a Cy box, a nuclear localization sequence, and a PEST sequence.

The term "Box Cy" refers to an amino acid sequence of about 5 amino acid residues in length having the consensus sequence RXHuF, characterized in that X is any amino acid and Hu is a hydrophobic uncharged amino acid such as Μ, I, L or V. Cy boxes are typically involved in the interaction of CKIs with cyclins.

The "nuclear localization sequence" refers to an amino acid sequence of about 4-20 amino acid residues in length, which serves to direct a protein to the nucleus. Typically, the nuclear localization sequence is rich in basic amino acids, such as arginine (R) and lysine (K). Nuclear localization signals are described in, for example, Gorlich D. (1998) EMBO 5.17: 2721-7. The CKI4 protein comprises multiple nuclear localization sequences.

A "PEST sequence" refers to an amino acid sequence that is enriched in the proline (P), glutamate (E), serine (S), and threonine (T) amino acid residues and is present in proteins with a high turnover rate. proteolytic. PEST sequences are described in, for example, Rogers et al. (1986) Science 234, 364-368.

The various structural domains in a CKI protein can be identified using specialized databases eg SMART (Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864; Letunic et al. (2002) Nucleic Acids Res 30, 242-244; http://smart.embl-heidelberg.de/), InterPro (Mulder et al., (2003) Nucl. Acids. Res. 31, 315-318; http: //www.ebi .ac.ukyinterpro /), Prosite (Bucher and Bairoch (1994), A generalized profile syntax for biomolecular sequences motifs and its fiinction in automatic sequence interpretation. (In) ISMB-94; Proceedings 2nd International Conference on Intelligent Systems for Molecular Biology. Altman R., Brutlag D., Karp P., Lathrop R., Searls D., Eds., Pp53-61, AAAIPress, Menlo Park; Hulo et al., Nucl. Acids Res. 32: D134-D137, ( 2004), http://www.expasy.org/prosite/) or Pfam (Bateman et al., Nucleic Acids Research 30 (1): 276-280 (2002),

http://www.sanger.ac.uk/Software/Pfam/).

In addition, a CKI protein may also be identifiable by its ability to inhibit the activity of a Cyclin Dependent Kinase (CDK), e.g., a plant CDK. CDKs are a group of serine / threonine kinases that regulate cell cycle progression in eukaryotes, e.g., plants. CDKs are typically complexed with cyclins forming an enzyme complex, CDK being the catalytic subunit and cyclin being the regulatory subunit of the enzyme complex (Wang, H. (1997) The Plant Journal 15 (4): 501-510).

Therefore by identifying a CKI polypeptide using one or more of the characteristics described above, one skilled in the art can readily derive the corresponding nucleic acid encoding the polypeptide, and use a sufficient length of substantially contiguous nucleotides thereof to perform any one or more of the above. gene silencing methods described above (for substantially reducing or eliminating expression of an endogenous CKI gene).

Preferred for use in the methods of the invention is a sufficient length of substantially contiguous nucleotides of SEQ ID NO: 267 (OsCKI4), or the use of a sufficient length of substantially contiguous nucleotides of a nucleic acid sequence encoding an OsCKI4 ortholog or paralog ( SEQ ID NO: 267). Examples of such OsCKI4 orthologs and paralogues are provided in

Table 13 below.

Orthologs and paralogues are homologues that encompass evolutionary concepts used to describe ancestral gene relationships. Paralogues are genes within the same species that originate through duplication of an ancestral gene and orthologs are genes from different organisms that originate through speciation.

Orthologists in, for example, monocotyledonous plant species can be easily found by performing a so-called reciprocal blast search. This can be done by first blasting involving blasting a query string (for example, SEQ ID NO: 267 or SEQ ID NO: 268) against any sequence database, such as the publicly available NCBI database that may be found at: http://www.ncbi.nlm.nih.gov. BLASTN or TBLASTX (using default default values) can be used when starting from a nucleotide sequence and BLASTP or TBLASTN (using default default values) can be used when starting from a protein sequence. BLAST results can optionally be filtered. Full length sequences of either the filtered results or unfiltered results are then BLASTed back (according to BLAST) against sequences from the organism from which the query sequence is derived (where the query sequence is SEQ ID NO: 267 or SEQ ID NO. : 268 the second blast should therefore be against rice strings). The results of the first and second BLASTs are then compared. A paralog is identified if a high-ranking second blast hit is of the same kind from which the query sequence is derived; An ortholog is identified if a high ranking hit is not of the same kind from which the query sequence is derived. High ranking hits are those with a low E value. The lower the E value, the more significant the score (or in other words the lower the chance that the hit was found at random). E-value computing is well known in the art. For large families, ClustalW can be used, followed by a neighboring tree to help visualize related gene grouping and to identify orthologs and paralogues.

Table 13 OsCKI4 Orthologs and Paralogues (SEQ ID NO: 267 and 268)

<table> table see original document page 179 </column> </row> <table>

The source of substantially contiguous nucleotides of a CKI gene / nucleic acid can be any plant source or artificial source.

For optimal performance, gene silencing techniques used for the substantial reduction or elimination of endogenous CKI gene expression require the use of monocotyledon plant CKI sequences for transformation into monocotyledonous plants. Preferably, CKI sequences of the Poaceae family are transformed into plants of the Poaceae family.

Still preferably, a rice CKI nucleic acid (be it a full length CKI sequence or a fragment) is transformed into a rice plant. CKI nucleic acid need not be introduced into the same plant variety. More preferably, the rice CKI nucleic acid is a sufficient length of substantially contiguous nucleotides of SEQ ID NO: 267 (OsCKI4) or a sufficient length of substantially contiguous nucleotides of a nucleic acid sequence encoding an OsCKI4 ortholog or paralog (SEQ ID NO: 267). As mentioned above, one of ordinary skill in the art should be well aware of what should constitute a sufficient length of substantially contiguous nucleotides to perform any of the above defined gene silencing methods, this may be as little as 20 or fewer substantially contiguous nucleotides in some. cases.

The invention also provides genetic constructs and vectors to facilitate introduction and / or expression of nucleotide sequences useful in the methods according to the invention.

Therefore, a gene construct comprising one or more control sequences capable of preferentially directing expression of a sense and / or antisense CKI nucleic acid sequence in plant endosperm tissue is provided to silence an endogenous CKI gene in human tissue. endosperm of a plant; and optionally a transcription termination sequence.

A preferred gene silencing construct is one comprising an inverted repeat of a CKI gene or fragment thereof, preferably capable of forming a staple structure, whose inverted repeat is under the control of a specific endosperm promoter.

Constructs useful in the methods according to the present invention may be constructed using recombinant DNA technology well known to those skilled in the art. Gene constructs may be inserted into commercially available vectors suitable for plant transformation and suitable for expression of the gene of interest in transformed cells. The invention therefore provides use of a gene construct as defined above in the methods of the invention.

The sequence of interest is operably linked to one or more control sequences (at least one promoter) capable of preferentially enhancing expression in plant endosperm tissue. The terms "regulatory element", "control sequence" and "promoter" are all used interchangeably in this place and are defined in the "Definitions" section in this place.

A specific endosperm promoter refers to any promoter capable of preferentially directing expression of the gene of interest in endosperm tissue. Reference herein to "preferentially" directing expression in endosperm tissue is adopted to mean directing expression of any sequence operably linked to it in endosperm tissue substantially to the exclusion of directing expression elsewhere in the plant, without regard to any residual expression due to to permeable promoter expression. For example, the prolamine promoter shows strong endosperm expression with meristem permeability, more specifically the bud meristem and / or center of discrimination in the meristem.

Preferably, the specific endosperm promoter is a promoter isolated from a prolamine gene, such as a rice RP6 prolamine promoter (Wen et al., (1993) Plant physiol 101 (3): 1115-6) as represented by SEQ ID. NO: 281 or a similar strength promoter and / or a promoter with a similar expression pattern as the rice prolamine promoter. Similar force and / or similar expression pattern can be analyzed, for example, by coupling promoters to a reporter gene and by checking reporter gene function in plant tissues. A well-known reporter gene is beta-glucuronidase and the GUS colorimetric dye used to visualize beta-glucuronidase activity in plant tissue. Examples of other specific endosperm promoters that may also be used to perform the methods of the invention are shown in Table 6 in the "Definitions" section herein.

Optionally, one or more terminator sequences may also be used in the construction introduced into a plant. The term "terminator" is as defined here in the "Definitions" section. The genetic constructs of the invention may additionally include an origin of replication sequence that is required for maintenance and / or replication in a specific cell type. An example is when a genetic construct is required to be maintained in a bacterial cell as an episomal genetic element (e.g., plasmid or cosmid molecule). Preferred sources of replication include, but are not limited to, fl-ori and colE1.

The genetic construct may optionally comprise a selectable marker gene as defined herein in the "Definitions" section.

The present invention also encompasses plants including plant parts obtainable by the methods according to the present invention having increased seed production relative to suitable control plants and which have reduced or substantially eliminated expression of an endogenous CKI gene in plant endosperm tissue.

The invention also provides a method for producing transgenic plants having increased seed production relative to suitable control plants whose transgenic plants have reduced or substantially eliminated expression of an endogenous CKI gene in plant endosperm tissue.

More specifically, the present invention provides a method for producing transgenic plants having increased seed production which method comprises:

(i) introducing and expressing in a plant, plant part or plant cell the gene construct comprising one or more control sequences capable of preferentially directing expression of a sense and / or antisense CKI nucleic acid sequence in plant endosperm tissue to silence an endogenous CKI gene in plant endosperm tissue; and

(ii) cultivate the plant, plant part or plant cell under conditions promoting plant growth and development.

Preferably, the construct introduced into a plant is one comprising an inverted (in part or complete) repeat of a CKI gene or fragment thereof, preferably capable of forming a staple structure.

According to a preferred feature of the present invention, the construction is introduced into a plant by transformation.

The term "transformation" is as defined in the "Definitions" section in this place.

The present invention clearly extends to any plant cell or plant produced by any of the methods described herein, and to all plant parts and seedlings thereof. The present invention further extends to encompass the progeny of a transformed or transfected primary cell, tissue, organ or whole plant that has been produced by any of the methods mentioned above, the only requirement being that the progeny exhibit the same (s) ) genotypic and / or phenotypic trait (s) than those produced by the ancestor in the methods according to the invention.

The invention also extends to harvestable parts of a plant such as seeds and derived products, preferably directly derived, from a harvestable part of such a plant, such as dried pellets or powders, oil, fat and fatty acids, starch. or proteins.

The present invention also encompasses use of CKI nucleic acids for the substantial reduction or elimination of endogenous CKI gene expression in plant endosperm tissue to increase plant seed production as defined above.

Description of figures

The present invention will now be described with reference to the following figures in which: Fig. 1 gives an overview of the conserved motifs present in SEQ ID NO: 2. Leucine rich domain is underlined, the conserved motifs 1, 2 and 3 are given in bold and the italic sequence represents the probable N-glycosylation site with the probable protein kinase C phosphorylation site.

Fig. 2 shows a multiple alignment of various SYR proteins. Asterisks indicate identical amino acid residues, colon represent highly conserved substitutions, and colon represent less conservative substitutions. With the information in Figure 1, the various domains and motifs conserved in SEQ ID NO: 2 can be easily identified in the other SYR proteins.

Fig. 3 shows binary vectors for transformation and Oryza sativa expression of an Oryza sativa SYR nucleic acid. In pGOS2 :: SYR, the SYR coding sequence is under the control of a rice GOS2 promoter.

Fig. 4 shows binary vectors for transformation and Oryza sativa expression of an Oryza sativa SYR nucleic acid. At pH MGP :: SYR, the SYR coding sequence is under the control of a rice HMGP promoter (SEQ ID NO: 18 in WO 2004/070039, whose SEQ ID NO: 18 of WO 2004/070039 is incorporated herein. as if completely exposed).

Fig. 5 details examples of sequences useful for carrying out the methods according to the present invention. SEQ ID NO: 1 and SEQ ID NO: 2 represent the nucleotide and protein sequence of SYRs used in the examples. Start and stop codons in SEQ ID NO: 1 are given in

bold. SEQ ID NO: 3 and SEQ ID NO: 4 are primer sequences used to isolate SYR nucleic acid. SEQ ID NO: 5 is the sequence of the GOS2 promoter and SEQ ID NO: 33 of the PROO170 promoter as used in the examples, SEQ ID NO: 6 through SEQ ID NO: 11 represent consensus sequences of conserved portions of the SYR proteins. SEQ ID NO: 12 to 25, 27 to 32 and 36 to 42 are nucleotide sequences (full or partial length) and proteins of the SYR gene and protein homologs as given in SEQ ID NO: 1 and SEQID NO: 2. SEQ ID NO: 26 represents the ARGOS protein sequence (GenBank accession AY305869).

Fig. 6 gives an overview of FG-GAP protein domains. The protein of SEQ ID NO: 46 comprises secretion signal (boxed N-terminal part), an FG-GAP domain beginning at P73 and ending with L98, indicated in bold and underlined, and a transmembrane domain (bold and boxed). The DXDXDGXX (DZE) conserved motif is boxed and underlined, characterized by the fact that the DGXX (D / E) motif is italicized. The conserved domain FDGYLYLID is underlined.

Fig. 7 shows a multiple alignment of full length FG-GAP proteins (SEQ ID NO: 46, SEQ ID NO: 55, SEQ ID NO: 57 and SEQ ID NO: 59), asterisks indicate identical amino acids, the colon indicate highly conserved substitutions and dots indicate less conservative substitutions. The partial sequences listed in Table G of Example 12 may be useful in such a multiple alignment for identifying additional motifs.

Fig. 8 shows a binary vector for transformation and Oryza sativa expression of a nucleic acid encoding Arabidopsis thaliana FG-GAP under the control of a rice GOS2 promoter.

Fig. 9 details examples of sequences useful for carrying out the methods according to the present invention. SEQ ID NO: 45 and SEQ ID NO: 46 represent the sequence of FG-GAP nucleotides and proteins used in the examples; start and stop codons in SEQ ID NO: 45 are given in bold. SEQ ID NO: 47 and SEQ ID NO: 48 are primer sequences used to isolate FG-GAP nucleic acid. SEQ ID NO: 49 is the sequence of the promoter-gene combination as used in the examples. SEQ ID NO: 50 to SEQ ID NO: 53 represent consensus sequences of conserved portions of FG-GAP proteins. SEQ ID NO: 54 to 71 are nucleotide sequences (full or partial length) and proteins of the FG-GAP gene and protein homologs as given in SEQ ID NO: 45 and SEQ ID NO: 46. SEQ ID NO: 72 is genomic sequence encoding a Medicago sativa FG-GAP protein whose protein comprises the peptide sequences represented by SEQ ID NO: 72 to 76.

Fig. 10 shows the important characteristics found in or homologous CYP90B polypeptides: the terminal-terminal hydrophobic domain, the transition domain (with the K / RK / R-X3-9-PGG, the AaD domains. Within domain A to Ala / Gly-Gly-X-Asp / Glu-Thr-Thr / Ser consensus sequence is identified.The Phe-Ala-Gly-His-Glu-Thr-Ser consensus sequence of the CYP90B polypeptides comprises this Ala / Gly consensus sequence. Gly-X-Asp / Glu-Thr-Thr / Ser.

Fig. 11 shows the branched brassinoesteroid biosynthetic pathway. In Arabidopsis, the CYP90B1 / DWF4 polypeptide comprises the enzymatic activity of steroid 22 alpha hydroxylase.

Fig. 12 shows the ProtScale production profile for hydrophobicity of the CYP90B polypeptide of the invention. The first 34 N-terminal (boxed) amino acids represent a hydrophobic domain as they are located above the zero delimiting line. This region corresponds to the N-terminal anchor domain.

Fig. 13 shows multiple alignment of various vegetable CYP90B polypeptides using the VNTI AlignX multiple alignment program based on a modified ClustalW algorithm (InforMax, Bethesda, MD, http://www.informaxinc.com) with default settings. for gap opening penalty of 10 and a gap length of 0.05. The N-terminal hydrophobic domain, the transition domain (with the K / R-K / R-X3.9-P-G-G) and the AaD domains are indicated. The Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence is boxed within domain A. The CYP90B polypeptide accession numbers can be found in Table 9a and 9b. Arath_CYP90Al_CPD (At5g05690), Arath_CYP90C 1 ROT3 (At4g36380) and Arath_CYP90D1 (At3gl3730) from Arabidopsis are shown as non-CYP90B polypeptides.

Fig. 14 shows a plant transformation vector for Oryza sativa expression of an Oryza sativa CYP90B nucleic acid under the control of a plant promoter, which may be a non-constitutive promoter (such as endosperm or specific embryo / aleurone) or a constitutive promoter (such as GOS2 and HMGB1).

Fig. 15 details examples of sequences useful for carrying out the methods according to the present invention. Several sequences result from public EST groupings (see Table 9a), with poor quality sequencing. As a consequence, a few nucleic acid substitutions may be expected. The start (ATG) and end codons delimit nucleic acid sequences when they are full length.

Fig. 16 is a schematic figure of a full length CDC27 polypeptide (more specifically the Arabidopsis thaliana hobbit polypeptide CDC27B). Tetratricopeptide repeats (TPR) are represented as black boxes. The NH2 terminal region of the polypeptide is represented as a black bar.

Fig. 17 shows the multiple alignment of CDC27 polypeptides from different sources using the VNTI AlignX multiple alignment program based on a modified ClustalW algorithm (InforMax, Bethesda, MD, http://www.informaxinc.com) with configurations. gap opening penalty of 10 and a gap length of 0.05. Tetratricopeptide repeats (TPR) are boxed through alignment. The conserved NH2 domain PDOl 1373 (as defined in ProDom, http://ribosome.toulouse.inra.fr/prodom/current/cgi-bin/ProDomBlast3.pl) is underlined twice.

Fig. 18 shows a pOSH1 :: CDC27 binary vector for expression in Oryza sativa of a modified Arabidopsis thaliana CDC27 nucleic acid under the control of a plant promoter which is a bud apical meristem promoter.

Fig. 19 shows a Table listing partial and full length CDC27 orthologs and paralogues from different sources produced by TIGR (Institute for Genomic Research at http://www.tigr.org). TC895803 can be found at http://www.tigr.org/tigr- scripts / tgi / ego / ego_report.pl? Ego = 895803.

Fig. 20 details examples of sequences useful for carrying out the methods according to the present invention, or useful for isolating such sequences. Several sequences result from public EST assemblies (see Table 10), with poor quality sequencing. As a consequence, a few nucleic acid substitutions may be expected. Start (ATG) and end codons delimit nucleic acid sequences when they encode full-length CDC27 polypeptides.

Fig. 21 shows a phylogenetic tree of various polypeptide sequences comprising an AT-hook domain and a DUF296 domain. The phylogenetic tree was made using VNTI AlignX multiple alignment program, based on a modified ClustalW algorithm (InforMax, Bethesda, MD, http://www.informaxinc.com), with default settings for gap gap 10 and a gap length of 0.05.

Fig. 22 shows a pPROLAMINE :: AT-hook binary vector for expression in Oryza sativa of an Oryza sativa nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 and Reason 2 domain under the control of a prolamine promoter. . Fig. 23 shows a multiple alignment of a polypeptide comprising an AT-hook domain and a DUF296 domain, prepared using AlignX VNTI multiple alignment program, based on a modified ClustalW algorithm (InforMax, Bethesda, MD, http: // www. informaxinc.com), with default settings for gap gap penalty of 10 and a gap length of 0.05. Shown in alignment are the AT-hook domain and the DUF296 and Reason 2 domain in bold, italics and underline.

Fig. 24 details examples of sequences useful for carrying out the methods according to the present invention.

Fig. 25 shows a phylogenetic tree of DOF transcription factors. The box near the top shows the main sequence grouping sharing homology with SEQ ID NO: 227 (and comprising characteristics (i) and (iii) as defined above, ie at least 60% of sequence identity or with the DOF domain represented SEQ ID NO: 200 or SEQ ID NO: 228; and Reason I and / or Reason II as defined above). The box closest to the bottom shows the main sequence grouping sharing homology with SEQ ID NO: 199 (and comprising characteristics (i) and (ii) as defined above, ie at least 60% of sequence identity or with the represented DOF domain SEQ ID NO: 200 or SEQ ID NO: 228, and at least 70% sequence identity with the DOF domain represented by SEQ ID NO: 200).

Fig. 26 shows a binary vector pGOS2 :: DOF for expression in Oryza sativa of an Arabidopsis thaliana DOF transcription factor under the control of a GOS2 promoter.

Fig. 27 shows a pPROLAMINE :: DOF binary vector for expression in Oryza sativa of an Arabidopsis thaliana DOF transcription factor under the control of a prolamine promoter.

Fig. 28 details examples of sequences useful for carrying out the methods according to the present invention.

Fig. 29 is a schematic representation of a full length plant CKI polypeptide. Typical motifs 1 through 5 (SEQ ID NO: 261 to SEQ ID NO: 265) useful for identifying CKIs are boxed and numbered accordingly (reason 6 not shown).

Fig. 30 shows a neighboring grouping tree of a multiple alignment of CKI polypeptides from different sources, and made using the ClustalW public computer application available at http://clustalw.genome.jp, with default settings. A subset of monocotyledon and dicotyledon CKI4s is indicated by the large bracket. Within this subgroup, monocotyledon CKIs group together, as indicated by the middle bracket. The monocotyledonous CKI4 branch is indicated by the small bracket.

Fig. 31 is a multiple alignment of CKI polypeptides from different plant sources, made using VNTI AlignX multiple alignment program, based on a modified ClustalW algorithm (InforMax, Bethesda, MD, http://www.informaxinc.com), with default settings for gap gap penalty of 10 and gap gap of 0.05. The conserved C-terminal end of CKIs are boxed, as are motifs 1 through 5 (SEQ ID NO: 261 to SEQ ID NO: 265) useful for identifying plant CKIs (motif 6 not shown).

Fig. 32 shows a binary vector for CKI RNA silencing in Oryza sativa using a staple construct under the control of a specific endosperm promoter and under the control of a specific bud promoter.

Fig. 33 details examples of sequences useful for carrying out the methods according to the present invention, or useful for isolating such sequences. Several sequences result from public EST assemblies, with poor quality sequencing. As a consequence, a few nucleic acid substitutions may be expected. Start (ATG) and end codons delimit nucleic acid sequences when they encode full-length CKI polypeptides. However both 5 'and 3' UTRs can also be used to perform the methods of the invention.

Examples

The present invention will now be described with reference to the following examples, which are by way of illustration only. The following examples are not intended to fully define or otherwise limit the scope of the invention.

DNA manipulation

Unless otherwise stated, recombinant DNA techniques are performed according to standard protocols described in (Sambrook (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition, Cold Spring Harbor Laboratory Press, CSH, New York) or in Volumes 1 and 2 of Ausubel et al. (1994), Current Protocols in Molecular Biology, Current Protocols (http://www.4ulr.com/products/currentprotocols/index.html).

Standard materials and methods for plant molecular work are described in Plant Molecular Biology Labfax (1993) by R.D.D. Croy, published by BIOS Scientific Publications Ltd (UK) and Blackwell Scientific Publications (UK).

Statistical analysis

A two-way ANOVA (analysis of variance) corrected for the unbalanced model was used as a statistical model for the general evaluation of plant phenotypic characteristics. An F test was performed on all measured parameters of all plants of all events transformed with that gene. The F-test was performed to check for a gene effect on all transformation events and to check for a general gene effect, also called here "global gene effect". If the value of the F test shows that the data is significant, then it is concluded that there is a "gene" effect, meaning that not only the presence or position of the gene is causing the effect. The threshold for significance for a true global gene effect is set at a 5% probability level for the F test.

To verify for an effect of genes within an event, i.e., for a specific lineage effect, a t-test was performed within each event using transgenic and corresponding null plant datasets. "Null plants" or "null segregants" or "nullizigotes" are plants treated in the same manner as the transgenic plant, but from which the transgene has been secreted. Null plants can also be described as negative transformed homozygous plants. The threshold for significance for the t-test is set at 10% probability level. Results for some events may be above or below this threshold. This is based on the hypothesis that a gene can only have an effect on certain positions in the genome, and that the occurrence of this position-dependent effect is not common. This type of gene effect is also called in this place a "gene lineage effect". The value of ρ is obtained by comparing the value of t with the distribution of t or alternatively by comparing the value of F with the distribution of F. The value of ρ then generates the probability that the null hypothesis (ie, that there is no transgene effect) is correct.

EXAMPLE A: SYR

Example 1: Identification of Sequences Related to SEQ ID NO: 1 and SEQID NO: 2

Sequences (full length cDNA, ESTs or genomic) related to SEQ ID NO: 1 and / or protein sequences related to SEQ ID NO: 2 were identified among those maintained in the Entrez Nucleotides database at the National Center for Biotechnology Information (NCBI) ) using database sequence search tools such as the Basic Local Alignment Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215: 403-410; and Altschul et al. (1997 ) Nucleic Acids Res. 25: 3389-3402). The program was used to find regions of local similarity between sequences by comparing nucleic acid or polypeptide sequences with sequence database and calculating the statistical significance of identities. The polypeptide encoded by SEQ ID NO: 1 was used for the TBLASTN algorithm, with default settings and the filter to bypass low complexity sequence counterweight. The result of the analysis was viewed by paired comparison, and ranked according to the probability score (E value), where the score reflects the probability that a particular alignment will occur at random (the lower the E value, the more significant the hit). . In addition to E values, comparisons were also scored by percentage identity. Percentage identity refers to the number of identical nucleotides (or amino acids) between the two nucleic acid (or polypeptide) sequences compared at a particular length. In some instances, the default parameters have been adjusted to modify the search stringency.

In addition to the publicly available nucleic acid sequences available from NCBI, other sequence databases can also be searched by following the same procedure as described above.

Table A provides a list of nucleic acid and protein sequences related to the nucleic acid sequence as represented by SEQ ID NO: 1 and the protein sequence represented by SEQ ID NO: 2.

Table A: Nucleic acid sequence related nucleic acid sequences (SEQ ID NO: 1) useful in the methods of the present invention, and the corresponding deduced polypeptides. <table> table see original document page 194 </column> </row> <table>

Example 2: Alignment of Relevant Polypeptide Sequences

Vector Nign AlignX (Invitrogen) is based on the popular Clustal progressive alignment algorithm (Thompson et al. (1997) Nucleic Acids Res 25: 4876-4882; Chenna et al. (2003). Nucleic Aeids Res 31: 3497-3500 ). A phylogenetic tree can be constructed using a neighbor grouping algorithm. Default values are for gap gap penalty of 10, gap gap penalty of 0.1 and the selected weight matrix is Blosum 62 (if polypeptides are aligned).

The result of multiple sequence alignment using relevant polypeptides to identify those useful for carrying out the methods of the invention is shown in Figure 2. Leucine-rich repeat and conserved motifs can be easily broken down into the various sequences.

Example 3: Calculating Global Percentage Identity Between Polypeptide Sequences Useful for Carrying Out the Methods of the Invention

Overall percentages of similarity and identity between full-length polypeptide sequences useful for carrying out the methods of the invention were determined using one of the methods available in the art, the MatGAT (BMC Bioinformatics. 2003 4:29) computer application. MatGAT: An application that generates similarity / identity matrices using protein or DNA sequences (Campanella JJ, Bitincka L, Smalley J; Ledion Bitincka hosted computer application). MatGAT computational application generates similarity / identity matrices for DNA or protein sequences without requiring data alignment. The program performs a series of paired alignments using the Myers and Miller global alignment algorithm (with a gap opening penalty of 12, and a gap length penalty of 2), calculating similarity and identity using for example Blosum 62 ( for polypeptides), and then put the results into a distance matrix. Sequence similarity is shown in the lower half of the dividing line and sequence identity is shown in the upper half of the diagonal dividing line.

Parameters used in the comparison were:

Score Matrix: Blosum62

First Gap: 12

Extension Gap: 2

Results of the computational application analysis are shown in Table B for similarity and overall identity at full length of polypeptide sequences (excluding partial polypeptide sequences). Percentage identity is given above the diagonal and percentage similarity is given below the diagonal.

Percent identity between polypeptide sequences useful for carrying out the methods of the invention may be as low as 27% amino acid identity compared to SEQ ID NO: 2. Table B

<table> table see original document page 191 </column> </row> <table> Example 4: Prediction of polypeptide topology sequences useful for carrying out the methods of the invention

TargetP 1.1 was used to predict the subcellular localization of eukaryotic proteins. According to the program, the location designation is based on the expected presence of any of the N-terminal pre-sequences: chloroplast transit peptide (cTP), mitochondrial targeting peptide (mTP) or secretory pathway signal peptide (SP) . Scores on which the final forecast is based are not real probabilities, and they do not necessarily add to one. However, the location with the highest score is most likely according to TargetP, and the relationship between the scores (the confidence class) may be an indication of how accurate the prediction is. Confidence class (RC) ranges from 1 to 5, where 1 indicates the strongest forecast. TargetP is maintained on the server of the Technical University of Denmark.

For predicted sequences containing an N-terminal pre-sequence a potential cleavage site may also be present.

Several parameters were selected, such as organism group (non-plant or plant), shortcut sets (none, predefined shortcut set, or user-specified shortcut set), and prediction calculation of cleavage sites (yes or not).

TargetP 1.1 analysis results of the polypeptide sequence as represented by SEQ ID NO: 2 are presented in Table C below. The "plant" organism group was selected, no shortcut defined, and the predicted length of the requested transit peptide.

According to the results, the subcellular location of the polypeptide sequence as represented by SEQ ID NO: 2 may be mitochondria; however the confidence class of 5 (i.e. the lowest confidence class) must be considered. Table C: TargetP 1.1 Analysis of Polypeptide Sequence as Represented by SEQ ID NO: 2

<table> table see original document page 199 </column> </row> <table>

Two transmembrane domains were identified by the TMHMM program hosted on the Center for Biological Sequence Analysis server, Technical University of Denmark. The results below show that the probability that the N terminus is located within is 0.997. Additional details in the orientation are given in Table D below. Table D: TMHMM 2.0 Results

<table> table see original document page 199 </column> </row> <table>

Many other algorithms can be used to perform such analyzes, including:

• ChloroP 1.1 hosted on the Technical University of Denmark server;

• Protein Prowler Subcellular Localization Predictor version 1.2 hosted on the Institute for Molecular Bioscience server, University of Queensland, Brisbane, Australia;

• PENCE Proteome Analyst PA-GOSUB 2.5 hosted on the University of Alberta server, Edmonton, Alberta, Canada;

Example 5: Gene Cloning

The Oryza sativa SYR gene was PCR amplified using an Oryza sativa seedling cDNA library (Invitrogen, Paisley, UK) as a template. After reverse transcription of seedling-extracted RNA, cDNAs were cloned into pCMV Sport 6.0. Average bank insert size was 1.5 kb and the original number of clones was on the order of 1.59 x 107 cfu. Original titer was determined to be 9.6 x 10 cfu / ml after first amplification of 6 x 10u cfu / ml. After plasmid extraction, 200 ng of template were used in a 50 μl PCR mix. Primers prm08170 (SEQ ID NO: 3; sense, bold start codon, AttBl site in italics: S ^ ggggacaagtttgtacaaaaaageag gcítaatfctfatggaaggtgtaggtgctagg-s') and prm08171 (complementary SEB ID: 2; Att; - ggggaccactttgtacaagaaagctgggtcaaaaacaaaaataaattcccc-3'1), which include the AttB sites for Gateway recombination, were used for PCR amplification. PCR was performed using Hifi Taq DNA polymerase under standard conditions. A PCR fragment of the correct size was amplified and purified using standard methods as well. The first step of the Gateway procedure, the BP reaction, was then performed, during which the PCR fragment recombines in vivo with plasmid pDONR201 to produce, according to Gateway terminology, an "input clone", pSYR. Plasmid pDONR201 was purchased from Invitrogen as part of Gateway® technology.

Example 6: Vector Construction

The input clone pSYR was subsequently used in an LR action with a destination vector used for Oryza sativa transformation. This vector contains as functional elements within the limits of T-DNA: a selectable plant marker; a traceable marker expression drawer; and a Gateway drawer intended for in vivo LR recombination with the sequence of interest already cloned into the input clone. A rice GOS2 promoter (SEQ ID NO: 5) for constitutive expression was located before this Gateway drawer. A similar vector construct was prepared, but with the High Mobility Group Protein promoter (HMGP, SEQ ID NO: 33) instead of the GOS promoter. After the LR recombination step, the resulting expression vectors, pGOS2 :: SYR (with the GOS2 promoter) and pH MGP :: SYR (with the HMGP promoter), both for constitutive expression of SYR (Figure 2) were transformed into lineage. Agrobacterium LBA4044 and subsequently for Oryza sativa plants. Example 7: Rice Processing

Agrobacterium containing the expression vector was used to transform Oryza sativa plants. Dried ripe seeds of the Japanese Nipponbare rice cultivar were peeled. Sterilization was performed by incubating for one minute in 70% ethanol, followed by 30 minutes in 0.2% HgCl2, followed by a 15-minute wash 6 times with sterile distilled water. The sterile seeds were then germinated in a medium containing 2,4-D (callus induction medium). After incubation in the dark for four weeks, scutellus-derived embryogenic calli were excised and propagated in the same medium. After two weeks, the corns were multiplied or sub-propagated in the same medium for another 2 weeks. Embryogenic callus pieces were subcultured in fresh medium 3 days prior to co-cultivation (to stimulate cell division activity).

Agrobacterium strain LBA4404 containing the expression vector was used for co-cultivation. Agrobaeterium was inoculated in AB medium with the appropriate antibiotics and grown for 3 days at 28 ° C. The bacteria were then collected and suspended in liquid co-cultivation medium to a density (OD60) of about 1. The suspension was then transferred to a petri dish and the calluses immersed in the suspension for 15 minutes. Callus tissues were then dried on filter paper and transferred to a solidified co-culture medium and incubated for 3 days in the dark at 25 ° C. Co-cultured calli were grown in medium containing 2,4-D- for 4 weeks in the dark at 28 ° C in the presence of a selection agent. During this period, rapidly growing hard-shelled islands developed. After transfer of this material to a regeneration medium and incubation in light, the embryogenic potential was released and shoots developed in the next four to five weeks. Sprouts were excised from the corns and incubated for 2 to 3 weeks in auxin-containing medium from which they were transferred to the soil. Hardened shoots were grown in high humidity and short days in a greenhouse.

Approximately 35 independent T0 rice transformants were generated for one construction. Primary transformants were transferred from a tissue crop camera to a greenhouse. After a quantitative PCR analysis to verify copy number of the T-DNA insert, only single copy transgenic plants exhibiting tolerance to the selection agent were maintained for T1 seed harvest. Seeds were then collected three to five months after transplantation. The method produced single locus transformants at a rate of up to 50% (Aldemita and Hodges 1996, Chan et al. 1993, Hiei et al. 1994).

For transformation of other crops see Example 40. Example 8: Methods of evaluating SYR-transformed plants under the control of the rice GOS2 promoter or the HMGP promoter

Trial Configuration

Approximately 15 to 20 independent TO transformants of rice were generated. Primary transformants were transferred from a tissue crop camera to a greenhouse for T1 seed growth and harvest. Eight events, of which T1 progeny segregated 3: 1 for presence / absence of transgene, were retained. For each of these events, approximately 10 T1 seedlings containing the transgene (hetero and homozygotes) and approximately 10 T1 seedlings lacking the transgene (nullizigotes) were selected by monitoring visual marker expression. The selected T1 plants were transferred to a greenhouse. Each plant received a unique barcode label to unambiguously link the phenotyping data to the corresponding plant. The selected Tl plants were grown in the soil in 10 cm diameter pots in the following environmental settings: photoperiod = 11.5 h, daylight intensity = 30,000 lux or more, day temperature = 28 ° C or higher, night = 22 ° C, relative humidity = 60-70%. Transgenic plants and the corresponding nullizigotes were grown side by side in random positions. From the sowing stage to the maturity stage the plants were passed several times through a cabinet for digital image formation. At each moment digital images (2048x1536 pixels, 16 million colors) were taken from each plant from at least 6 different angles.

Saline Stress Screening

4-event plants (T2 seeds) were grown on a substrate made of coconut and argex fibers (3 to 1 ratio). A normal nutrient solution was used for the first two weeks after transplanting the seedlings in the greenhouse. After the first two weeks, 25 mM salt (NaCl) was added to the nutrient solution until the plants were collected.

Drought Screening

Five-event plants (T2 seeds) were grown in pot soil under normal conditions until they reached the spike stage. They were then transferred to a "dry" section where irrigation was retained. Moisture probes were inserted into randomly selected pots to monitor soil water content (SWC). When SWC passed below certain limits, the plants were automatically re-irrigated continuously until a normal level was reached again. The plants were then re-transferred to normal conditions. The rest of the crop (plant maturation, seed collection) was the same as for plants not grown under abiotic stress conditions. A confirmation session was carried out consisting of repeating the screening with T2 seeds not collected from plants of the first drought, but from plants grown under normal conditions.

Measured Parameters

Plant shoot (or leaf biomass) was determined by counting the total number of pixels in the plant shoot digital images broken down from the background. This value was averaged for photos taken at the same time from different angles and converted to a physical surface value expressed in mm squared by calibration. Experiments show that the plant shoot measured in this way correlates with the plant shoot biomass. Areamax is the shoot at the moment the plant reached its maximum leaf biomass.

The mature primary panicles were collected, bagged, bar-coded and then oven dried at 37 ° C for three days. The panicles were then traced and all seeds collected. The full shells were separated from the empty shells using an air blowing device. After separation, both seed lots were then counted using a commercially available counting machine. The empty shells were discarded. The full shells were weighed on an analytical balance and the cross sectional area of the seeds was measured using digital imaging. This procedure resulted in the set of the following seed related parameters:

Panicle flowers estimate the average number of rapiers per panicle in a plant, derived from the total number of seeds divided by the number of first panicles. The highest panicle and all panicles that overlap with the highest panicle when vertically aligned were considered the first panicles and were counted manually. The number of full grains was determined by counting the number of full husks that remained after the separation step. Total seed yield (total seed weight) was measured by weighing all full bark collected from a plant. Total number of seeds per plant was measured by counting the number of bark collected from a plant and corresponds to the number of rapiers per plant. Thousand Seed Weight (TKW) is extrapolated from the number of full grains counted and their total weight. Harvest index is defined as the ratio of total seed weight to surface area (mm2) multiplied by a factor 10 ^ 6. The EmerVigor parameter is an indication of seedling vigor. It is calculated from the area (in mm2) covered by leaf biomass in the first imaging. Seed filling rate (filling rate) is an indication of seed filling. It is expressed as a ratio (in%) of the number of filled grains by the number of rapiers (in total seeds).

These parameters were derived in an automated manner from digital images using computational image analysis application and were statistically analyzed. Individual seed parameters (including width, length, area, weight) were measured using a custom device consisting of two main components, a weighing and imaging device coupled with a computer application for image analysis.

Example 9: Measurement of production related parameters for pGOS2 :: SYR transformants grown under normal growing conditions:

Through seed analysis as described above, applicants found that plants transformed with the pGOS2 :: STR gene construct had a higher seed yield, expressed as number of full grains, total seed weight and harvest index, compared to plants lacking transgene SYR. The values of ρ show that the increases were significant. Methods for statistical analysis are as given in the introductory section for the Examples.

The results obtained for plants in generation T1 are summarized in Table E, which represents the mean values for all tested strains:

Table E:

<table> table see original document page 206 </column> </row> <table>

The data obtained for SYR in the first experiment were confirmed in a second experiment with T2 plants. Four strains that had the correct expression pattern were selected for further analysis. Seed groups of positive plants (both hetero and homozygous) in T1 were screened by monitoring marker expression. For each chosen event, the heterozygous seed groups were then retained for T2 evaluation. Within each seed group an equal number of positive and negative plants were grown in the greenhouse for evaluation. Measurement of seed yield parameters again showed increase in full grain number, total seed weight and harvest index compared to plants lacking the S YR transgene.

Example 10: Measurement of production-related parameters for transformantspGOS2 :: SYR grown under stress conditions:

Through seed analysis as described above, applicants found that plants transformed with the pGOS2 :: SYR gene construct and grown under saline stress had a higher seed yield, expressed as number of full grains, total seed weight, fill rate. and harvest index, compared to plants lacking the SYR transgene. In addition, these salt-stressed plants had a higher seedling vigor compared to control plants. When the plants were grown under drought stress, the transgenic plants had a higher total seed weight and an increased harvest index compared to plants lacking the SYR transgene. These differences were significant, with a P-value of test F below 0.05.

Example 11: Measurement of Production-Related Parameters for MGP :: SYR: pH Transformants

Similarly as for plants transformed with the pGOS2 :: SYR gene construct, applicants have found that plants transformed with the pH MGP :: SYR gene construct had a higher seed yield, expressed as full grain number, total seed weight and index. compared to plants lacking the SYR transgene. The values of ρ show that the increases were significant.

The results obtained for plants in generation T1 are summarized in Table F, which represents the mean values for all strains tested:

Table F:

<table> table see original document page 207 </column> </row> <table>

EXAMPLE B: FG-GAP

Example 12: Identification of Sequences Related to SEQID NO: 45 and SEQID NO: 46

Sequences (full length cDNA, ESTs or genomic) related to SEQ ID NO: 45 and / or protein sequences related to SEQ ID NO: 46 were identified among those maintained in the Entrez Nucleotides database at the National Center for Biotechnology Information (NCBI) ) using database sequence search tools such as the Basic Local Alignment Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215: 403-410; and Altschul et al. (1997 ) Nucleic Acids Res. 25: 3389-3402). The program was used to find regions of local similarity between sequences by comparing nucleic acid or polypeptide sequences with sequence database and calculating the statistical significance of identities. The polypeptide encoded by SEQ ID NO: 45 was used for the TBLASTN algorithm, with default settings and the filter to bypass low complexity sequence counterweight. The result of the analysis was viewed by paired comparison, and ranked according to the probability score (E value), where the score reflects the likelihood that a particular alignment will occur at random (the lower the E value, the more significant the hit). . In addition to E values, comparisons were also scored by percentage identity. Percentage identity refers to the number of identical nucleotides (or amino acids) between the two nucleic acid (or polypeptide) sequences compared at a particular length. In some instances, the default parameters may be adjusted to modify the search stringency.

In addition to the publicly available nucleic acid sequences available from NCBI, other sequence databases can also be searched by following the same procedure as described above.

Table G provides a list of nucleic acid and protein sequences related to the nucleic acid sequence as represented by SEQ ID NO: 45 and the protein sequence represented by SEQ ID NO: 46. Table G: Nucleic acid sequences related to the sequence nucleic acids (SEQ ID NO: 45) useful in the methods of the present invention, and the corresponding deduced polypeptides.

<table> table see original document page 209 </column> </row> <table>

Example 13: Alignment of Relevant Polypeptide Sequences

Vector Nign AlignX (Invitrogen) is based on the popular Clustal progressive alignment algorithm (Thompson et al. (1997) Nucleic Acids Res 25: 4876-4882; Chenna et al. (2003). Nucleic Acids Res 31: 3497-3500 ). A phylogenetic tree can be constructed using a neighbor grouping algorithm. Default values are for gap gap penalty of 10, gap gap penalty of 0.1 and the selected weight matrix is Blosum 62 (if polypeptides are aligned).

The result of multiple sequence alignment using relevant polypeptides to identify those useful for carrying out the methods of the invention is shown in Figure 7. One can clearly see that despite some alignment gaps, sequence conservation is found throughout most of the sequence. of proteins.

Example 14: Calculation of Global Percentage Identity Between Polypeptide Sequences Useful for Carrying Out the Methods of the Invention

Overall percentages of similarity and identity between full-length polypeptide sequences useful for carrying out the methods of the invention were determined using one of the methods available in the art, the MatGAT (BMC Bioinformatics. 2003 4:29) computer application. MatGAT: An application that generates similarity / identity matrices using protein or DNA sequences (Campanella JJ, Bitincka L, Smalley J; Ledion Bitincka hosted computer application). MatGAT computational application generates similarity / identity matrices for DNA or protein sequences without pre-alignment of data. The program performs a series of paired alignments using the Myers and Miller global alignment algorithm (with a gap opening penalty of 12, and a gap length penalty of 2), calculating similarity and identity using for example Blosum 62 ( for polypeptides), and then put the results into a distance matrix. Sequence similarity is shown in the lower half of the dividing line and sequence identity is shown in the upper half of the diagonal dividing line.

Parameters used in the comparison were: Score Matrix: Blosum62 FirstGap: 12 Extension Gap: 2

Results of the computational application analysis are shown in Table H for similarity and overall identity at full length of polypeptide sequences (excluding partial polypeptide sequences). Percentage identity is given above the diagonal and percentage similarity is given below the diagonal.

Percentage identity between polypeptide sequences useful for carrying out the methods of the invention may be as low as 17% amino acid identity compared to SEQ ID NO: 46. Table H: MatGAT Results for Similarity and Overall Identity at Full Length of polypeptide sequences.

<formula> formula see original document page 211 </formula>

Example 15: Identification of domains comprised of polypeptide sequences useful for carrying out the methods of the invention.

The Integrated Resource of Protein Families, Domains and Sites (InterPro) database is an integrated interface for signature databases commonly used for text and string-based searches. The InterPro database combines these databases, which use different methodologies and varying degrees of well-characterized protein biological information to derive protein signatures. Collaborating databases include SWISS-PROT, PROSITE, TrEMBL, PRINTS, ProDom and Pfam, Smart and TIGRFAMs. Interpro is hosted at the European Bioinformatics Institute in the United Kingdom.

InterPro scan results of the polypeptide sequence as represented by SEQ ID NO: 46 are presented in Table I.

Table I: InterPro Scan Results of Polypeptide Sequence as Represented by SEQ ID NO: 46

<table> table see original document page 212 </column> </row> <table>

Example 16: Topology Prediction of Polypeptide Sequences Useful for Carrying Out the Methods of the Invention

TargetP 1.1 predicts the subcellular localization of eukaryotic proteins. The location designation is based on the expected presence of any of the N-terminal pre-sequences: chloroplast transit peptide (cTP), mitochondrial targeting peptide (mTP) or secretory pathway signal peptide (SP). Scores on which the final forecast is based are not real probabilities, and they do not necessarily add to one. However, the location with the highest score is most likely according to TargetP, and the relationship between the scores (the confidence class) may be an indication of how accurate the prediction is. Confidence class (RC) ranges from 1 to 5, where 1 indicates the strongest forecast. TargetP is maintained on the server of the Technical University of Denmark.

For predicted sequences to contain an N-terminal pre-sequence a potential cleavage site may also be predicted.

Several parameters were selected, such as organism group (non-plant or plant), shortcut sets (none, predefined shortcut set, or user-specified shortcut set), and cleavage site prediction calculation (yes or not).

TargetP 1.1 analysis results of the polypeptide sequence as represented by SEQ ID NO: 46 are presented in Table J. The "plant" organism group was selected, no shortcuts defined, and the predicted length of the requested transit peptide. The subcellular location of the polypeptide sequence as represented by SEQ ID NO: 46 is probably not intracellular, there is a slight preference for the secretory pathway (although with a reliable score of 5) and the predicted putative transit peptide length is 24 amino acids. starting from the N-terminus (not as reliable as predicting the subcellular location itself, may vary in length from a few amino acids).

Table J: TargetP 1.1 Analysis of Polypeptide Sequence as Represented by SEQ ID NO: 46

<table> table see original document page 213 </column> </row> <table>

When analyzed with SignalP (Bendtsen et al., J. Mol. Biol., 340: 783-795, 2004), there is a reliable positive identification (probability of 0.998) for the presence of an N-terminal secretion signal peptide with a 24 amino acid in length. In addition, when using the Center for Biological Sequence Analysis, Technical University of Denmark (THMM) algorithm, the protein is predicted to be located on the outside of the cell with only one C-terminal tail in the cytoplasm: residues 1-859: outside; residues 860-879: transmembrane domain, residues 880-896: within.

Many other algorithms can be used to perform such analyzes, including:

• ChloroP 1.1 hosted on the Technical University of Denmark server;

• Protein Prowler Subcellular Localization Predictor version 1.2 hosted on the Institute for Molecular Bioscience server, University of Queensland, Brisbane, Australia; • PENCE Proteome Analyst PA-GOSUB 2.5 hosted on the University of Alberta server, Edmonton, Alberta, Canada;

Example 17: Gene Cloning

The Arabidopsis thaliana FG-GAP gene was PCR amplified using a template of an Arabidopsis thaliana seedling cDNA library (Invitrogen, Paisley, UK). After reverse transcription of seedling-extracted RNA, cDNAs were cloned into pCMV Sport 6.0. Average bank insert size was 1.5 kb and the original number of clones was on the order of 1.59 x 10 7 cfu. Original titer was determined to be 9.6 x 10 5 cfu / ml after first amplification of 6 x 10 11 cfu / ml. After plasmid extraction, 200 ng of template were used in a 50 μl PCR mix. Primers prm06643 (SEQ ID NO: 47; sense, bold start codon, AttBl site in italics: 5'-ggggacaagtttgtacaaaaaagcaggcttaaacaatgaaatctcgagcgagg-3 ') and prm06644 (SEQ ID no: 48; reversed Att: 2 - ggggaccactttgtacaagaaagctgggtcctg tttacagatggtacctagt-3 '), which include AttB sites for Gateway recombination, were used for PCR amplification. PCR was performed using Hifi Taq DNA polymerase under standard conditions. A 3.2 kb PCR fragment (including attB sites) was amplified and purified using standard methods as well. The first step of the Gateway procedure, the BP reaction, was then performed, during which the PCR fragment recombines in vivo with plasmid pDONR201 to produce, according to Gateway terminology, an "input clone", pFG-GAP. . Plasmid pDONR201 was purchased from Invitrogen as part of Gateway® technology.

Example 18: Vector Construction

The pFG-GAP input clone was subsequently used in an LR reaction with pGOS2, a destination vector used for Oryza sativa transformation. This vector contains as functional elements within the limits of T-DNA: a selectable plant marker; a traceable marker expression drawer; and a Gateway drawer intended for in vivo LR recombination with the sequence of interest already cloned into the input clone. A rice GOS2 promoter (nucleotides 1 through 2193 of SEQ ID NO: 49, the promoter-gene combination) for constitutive expression was located prior to this Gateway cassette.

After the LR recombination step, the resulting expression vector, pGOS2 :: FG-GAP for FG-GAP (Figure 7) was transformed into Agrobacterium strain LBA4044 and subsequently to Oryza sativa plants. Transformed rice plants were allowed to grow and were then evaluated for the parameters described in Example 19.

For transformation of other crops see Example 40. Example 19: Evaluation methods for FG-GAP transformed plants under the control of the GOS2 rice promoter

Approximately 15 to 20 independent T0 rice transformants were generated. Primary transformants were transferred from a tissue crop camera to a greenhouse for T1 seed growth and harvest. Seven events, of which T1 progeny segregated 3: 1 for presence / absence of transgene, were retained. For each of these events, approximately 10 T1 seedlings containing the transgene (hetero and homozygotes) and approximately 10 T1 seedlings lacking the transgene (nullizigotes) were selected by monitoring visual marker expression. The selected T1 plants were transferred to a greenhouse. Each plant was given a unique barcode label to unambiguously link the phenotyping data to the corresponding plant. The selected T1 plants were grown in the soil in pots of 10 cm in diameter in the following environmental settings: photoperiod = 11.5 h, daylight intensity = 30,000 lux or more, day temperature = 28 ° C or higher, night = 22 ° C, relative humidity = 60-70%. Transgenic plants and the corresponding nullizigotes were grown side by side in random positions. From the sowing stage to the maturity stage the plants were passed several times through a digital imaging booth. At each moment digital images (2048x1536 pixels, 16 million colors) were taken from each plant from at least 6 different angles.

Plant shoot (or leaf biomass) was determined by counting the total number of pixels in the digital images of surface plant parts broken down from the bottom. This value was averaged for photos taken at the same time from different angles and converted to a physical surface value expressed in mm squared by calibration. Experiments show that the plant shoot measured in this way correlates with the plant shoot biomass. Areamax is the shoot at the moment the plant reached its maximum leaf biomass.

The mature primary panicles were collected, bagged, bar-coded and then oven dried at 37 ° C for three days. The panicles were then traced and all seeds collected. The full shells were separated from the empty shells using an air blowing device. After separation, both seed lots were then counted using a commercially available counting machine. The empty shells were discarded. The full shells were weighed on an analytical balance and the cross sectional area of the seeds was measured using digital imaging. This procedure resulted in the set of the following seed related parameters:

Flowers per panicle is a parameter estimating the average number of rapiers per panicle in a plant, derived from the total number of seeds divided by the number of first panicles. The highest panicle and all panicles that overlap with the highest panicle when vertically aligned were considered the first panicles and were counted manually. The number of full grains was determined by counting the number of full husks that remained after the separation step. Total seed yield (total seed weight) was measured by weighing all full bark collected from a plant. Total number of seeds per plant was measured by counting the number of bark collected from a plant and corresponds to the number of rapiers per plant. Thousand Seed Weight (TKW) is extrapolated from the number of full grains counted and their total weight. Harvest index is defined as the ratio of total seed weight to surface area (mm2), multiplied by a factor of 106. These parameters were derived in an automated manner from digital images using computational image analysis application and were statistically analyzed. Individual seed parameters (including width, length, area, weight) were measured using a custom device consisting of two main components, a weighing and imaging device coupled with a computer application for image analysis.

A two-way ANOVA (analysis of variance) corrected for the unbalanced model was used as a statistical model for the general evaluation of plant phenotypic characteristics. An F test was performed on all measured parameters of all plants of all events transformed with that gene. The F test was performed to check for a gene effect on all transformation events and to check for a general gene effect, also referred to here as a "global gene effect". If the value of the F test showed that the data were significant, then it was concluded that there was a "gene" effect, meaning that it was not just the presence or position of the gene that was causing the effect. The threshold for significance for a true global gene effect was set at a 5% probability level for the F test. To verify for an effect of genes within an event, ie, for a specific lineage effect, a t test was performed. within each event using data sets of the transgenic plants and the corresponding null plants. "Null plants" or "null segregants" or "nullizigotes" refer to plants treated in the same manner as the transgenic plant but from which the transgene has been secreted. Null plants can also be described as negative transformed homozygous plants. The threshold for significance for the t-test was set at a 10% probability level. Results for some events may be above or below this threshold. This is based on the hypothesis that a gene can only have an effect on certain positions in the genome, and that the occurrence of this position-dependent effect is not common. This type of gene effect is also referred to here as a "gene lineage effect". The value of ρ was obtained by comparing the value of t with the distribution of t or alternatively by comparing the value of F with the distribution of F. The value of ρ then generates the probability that the null hypothesis (ie, that there is no transgene effect) is correct.

The data obtained for FG-GAP in the first experiment were confirmed in a second experiment with T2 plants. Four strains were selected for further analysis. Seed groups of positive plants (both hetero and homozygous) in IT were screened by monitoring marker expression. For each chosen event, the heterozygous seed groups were then retained for T2 evaluation. Within each seed group an equal number of positive and negative plants were grown in the greenhouse for evaluation.

A total of 120 FG-GAP transformed plants were evaluated in the T2 generation, ie 30 plants per event of which 15 were transgene positive and 15 negative.

Due to the fact that two experiments with overlapping events were performed, a combined analysis was performed. This is useful for verifying consistency of effects on both experiments, and if so, for accumulating evidence from both experiments to increase confidence in the conclusion. The method used was a mixed model approach that takes into account the multivariate data structure (i.e. experiment - event - segregants). P values were obtained comparing probability ratio test with chi square distributions. Example 20: Evaluation of FG-GAP Transformants: Measurement of Production-Related Parameters

Through seed analysis as described above, the applicants found that plants transformed with the FG-GAP gene construct had a higher seed yield, expressed as full grain number and total seed weight, compared to plants lacking the FG-GAP transgene. The values of ρ show that the increases were significant. Also the harvest index was increased (+ 9%).

The results obtained for plants in generation T1 are summarized. Table K:

Table K:

<table> table see original document page 219 </column> </row> <table>

These positive results were obtained again in the T2 generation. In Table L, data show the overall increases in% for the number of full grains, total seed weight and harvest index, calculated from the data of individual T2 generation strains, and the respective p values. These T2 data were reassessed in a combined analysis with the T1 generation results, and the ρ values obtained show that the observed effects were highly significant. Table L:

<table> table see original document page 220 </column> </row> <table>

EXAMPLE C: CYP90B

Example 21: Oryza sativa CYP90B cDNA Gene Cloning

Oryza sativa CYP90B cDNA was PCR amplified using a model Oryza sativa seedling cDNA library (Invitrogen, Paisley, UK). After reverse transcription of seedling-extracted RNA, cDNAs were cloned into pCMV Sport 6.0. Average bank insert size was 1.6 kb and the original number of clones was around 1.67x107 cfu. Original titer was determined to be 3.34 x 10 8 cfu / ml after the first amplification of 6 x 10 10 cfu / ml. After plasmid extraction, 200 ng of template were used in a 50 μΐ PCR mix. Primers (SEQ ID NO: 107; meaning, bold start codon, AttBl site in italics: 5 '

GGGGA CAA GTTTGTA CAAAAA The GCA GGCTTAAAC AATGGCCGCC ATG ATGGC 3 ') and (SEQ ID NO: 108; reverse, complementary site AttB2 in italics: 5' GGGGACCACTTTGTACAAGAAAGCTGGGT TTACTATB the included Attach sites, the recommended ATC sites, the recommended ATATGTB Gateway) for PCR amplification. PCR was performed using Hifi Taq DNA polymerase under standard conditions. A 1585 bp PCR fragment (including attB; 1521 bp start-to-finish sites) was amplified and purified using standard methods as well. The first step of the Gateway procedure, the BP reaction, was then performed, during which the PCR fragment recombines in vivo with plasmid pDONR201 to produce, according to Gateway terminology, an "input clone". Plasmid pDONR2Q1 was purchased from Invitrogen as part of Gateway® technology. Example 22: Vector Construction

The input clone was subsequently used in an LR reaction with destination vectors used for Oryza sativa transformation. These vectors contain as functional elements within the limits of T-DNA: a selectable plant marker; a traceable marker expression drawer; and a Gateway drawer intended for in vivo LR recombination with the sequence of interest already cloned into the input clone. Four different rice promoters located before this Gateway drawer were used to express Oryza sativa CYP90B: prolamine RP6, oleosin 18 kDa, GOS2 and HMGB1.

Following the LR recombination step, the resulting expression vectors (RP6 prolamine promoter, 18 kDa oleosin, GOS2 and HMGB1 - see Figure 14) were transformed into LBA4044 Agrobacterium strain and subsequently to Oryza sativa plants. Transformed rice plants were allowed to grow and were then evaluated for the parameters described in the examples below. For transformation of other crops see

Example 40.

Example 23: Description of the phenotypic assessment procedure

Approximately 15 to 20 independent rice TO transformants were generated per construct. Primary transformants were transferred from a tissue crop camera to a greenhouse for T1 seed growth and harvest. Four or five events, of which T1 progeny segregated 3: 1 for presence / absence of the transgene, were retained. For each of these events, approximately 10 T1 seedlings containing the transgene (hetero and homozygous) and approximately 10 T1 seedlings lacking the transgene (nullizigotes) were selected by monitoring visual marker expression. Suitable transgenic plants and control plants were grown side by side at random positions. From the sowing stage to the maturity stage the plants were passed several times through a digital imaging booth. At each moment digital images (2048x1536 pixels, 16 million colors) were taken from each plant from at least 6 different angles.

Three T1 events were additionally evaluated in generation T2 following the same evaluation procedure as for generation T1 but with more individuals per event.

Seed related parameter measurements

The mature primary panicles were collected, counted, bagged, barcoded and then dried for three days in an oven at 37 ° C. The panicles were then traced and all seeds were collected and counted. The full shells were separated from the empty shells using an air blowing device. The empty shells were discarded and the remaining fraction counted again. The full shells were weighed on an analytical balance. The number of full grains was determined by counting the number of full husks that remained after the separation step. Total seed yield was measured by weighing all full bark collected from a plant. Total number of seeds per plant was measured by counting the number of bark collected from a plant. Thousand Seed Weight (TKW) is extrapolated from the number of full grains counted and their total weight. The harvest index (HI) in the present invention is defined as the ratio of total seed yield to shoot (mm2) multiplied by a factor 106. The total number of flowers per panicle as defined in the present invention is the ratio of the total number of seeds to the number of mature primary panicles. The seed fill rate as defined in the present invention is the ratio (expressed as a%) of the number of full grains to the total number of seeds (or rapiers). Individual seed parameters (width, length and area) were measured using a custom device consisting of two main components, a weighing and imaging device coupled with a computational application for image analysis. Both shelled and husked seeds were used for these measurements. Statistical analysis: F test

A two-way ANOVA (variant analysis) was used as a statistical model for the general evaluation of plant phenotypic characteristics. An F test was performed on all measured parameters of all plants of all events transformed with the gene of the present invention. The F test was performed to check for a gene effect on all transformation events and for a general gene effect, also known as a global gene effect. The threshold for significance for a true global gene effect has been adjusted to a 5% probability level for the F test. A significant F test value points to a gene effect, meaning that it is not just the presence or position of the gene that is present. causing differences in phenotype.

Example 24: Results of Oryza sativa CYP90B under the control of non-constitutive promoters

24.1 Transgenic plants expressing CYP90B under control of specific endosperm promoter

The results of seed production and HI measurement for transgenic plants expressing CYP90B under the control of specific endosperm promoter (prolamine RP6) are shown in Table MeN, respectively. The number of events with an increase is indicated, as well as the ρ values of the F test for Tle T2 generations. Table Μ: Results of measurement of seed yield of transgenic plants expressing CYP90B under the control of specific endosperm promoter.

<table> table see original document page 224 </column> </row> <table>

Table N: IH measurement results from transgenic plants expressing CYP90B under the control of the specific endosperm promoter.

<table> table see original document page 224 </column> </row> <table>

Transgenic rice plants expressing CYP90B under the control of specific endosperm promoter (prolamine RP6) show an increased crop due to an increase in seed yield while plant shoot biomass remains unmodified (data not shown) when compared. with control plants.

24.2 Transgenic plants expressing CYP90B under the control of the embryo / specific aleurone promoter

TKW measurement results for transgenic plants expressing CYP90B under the control of an embryo / aleurone promoter (oleosin 18 kDa) are shown in Table O. The number of events with an increase is indicated as well as the ρ values of the F test. for generations T1 and T2.

Table O: TKW measurement results from transgenic plants expressing CYP90B under the control of the embryo / aleurone promoter.

<table> table see original document page 224 </column> </row> <table>

Mean seed area measurement results for transgenic plants expressing CYP90B under the control of the 18 kDa oleosin promoter are shown in Table Ρ. The number of events with an increase is indicated as well as the F values of test F for generations T1 and T2.

Table P: Measurement results of mean seed area of transgenic plants expressing CYP90B under control of embryo / aleurone promoter.

<table> table see original document page 225 </column> </row> <table>

The mean seed length measurement results for transgenic plants expressing CYP90B under the control of the 18 kDa oleosin promoter are shown in Table Q. The number of events with an increase is indicated as well as the ρ values of the F test for generations. T1 and T2.

Table Q: Results of mean seed length measurement of transgenic plants expressing CYP90B under control of embryo / aleurone promoter.

<table> table see original document page 225 </column> </row> <table>

Transgenic rice plants expressing CYP90B under the control of an embryo / aleurone promoter (oleosin 18 kDa) have seeds with increased TKW, seed area and seed length. No significant increase in seed yield was observed.

Example 25: Evaluation and Results of Oryza sativa CYP90B under the Control of Constitutive Promoters

25.1 Transgenic plants expressing CYP90B under the control of the constitutive promoter GOS2

Evaluation measurement results for transgenic plants expressing CYP90B under the control of the constitutive promoter GOS2 are shown in Table R. The number of events with an increase is indicated, as well as the ρ values of the F test for the TI generation. No T2 generation evaluation is performed when negative results are obtained in TI generation.

Table R: Evaluation results of evaluation of transgenic plants expressing CYP90B under the control of the constitutive promoter GOS2.

<table> table see original document page 226 </column> </row> <table>

25.2 Transgenic plants expressing CYP90B under the control of the constitutive promoter HMBGl

Evaluation measurement results for transgenic plants expressing CYP90B under the control of the constitutive promoter HMBGl are shown in Table S. The number of events with an increase is indicated, as well as the ρ values of the F test for the TI generation. No T2 generation evaluation is performed when negative results are obtained in TI generation.

Table S: Measurement results of evaluation of transgenic plants expressing CYP90B under the control of the constitutive promoter HMBGl.

<table> table see original document page 226 </column> </row> <table>

Transgenic rice plants expressing CYP90B under the control of two different constituent promoters show strongly reduced plant shoot biomass, plant height, full grain number, seed yield and HI compared to control plants.

EXAMPLE D: CDC27

Example 26: Cloning of an Arabidopsis thaliana gene encoding a CDC27 polypeptide having at least one inactive TPR domain in the NH2 terminal region of the polypeptide.

The Arabidopsis thaliana gene encoding a CDC27 polypeptide having at least one inactive TPR domain in the polypeptide terminal NH2 region (CDS0171_2) was PCR amplified using an Arabidopsis thaliana seedling cDNA library (Invitrogen, Paisley, UK). After reverse transcription of seedling-extracted RNA, cDNAs were cloned into pCMV Sport 6.0. Average bank insert size was 1.5 kb and the original number of clones was around 1.59x10 cfu. Original titer was determined to be 9.6x10 5 cfu / ml, and after the first 1010 cfu / ml amplification. After plasmid extraction, 200 ng of template were used in a 50 μΐ PCR mix. Primers (SEQ ID NO: 149; sense, bold start codon, AttBl site in italics: 5'- GGGGA CAA GTTTGTA CAAAA AA GCA GGCTTC AC ATGC AAC AA CTGTCAACTTC 3 ') and (SEQ ID NO: 150; reverse, complementary, AttB2 site in italics: 5 'GGGGACCA CTTTGTACAAGAAAGCTGGGTTGGAG TAGC TATGGTTTCAC-3'), which includes AttB sites for Gateway recombination, were used for PCR amplification. PCR was performed using Hifi Taq DNA polymerase under standard conditions. An 1816 bp PCR fragment (including attB; 1737 bp start-to-finish sites) was amplified and purified using standard methods as well. The first step of the Gateway procedure, the BP reaction, was then performed, during which the PCR fragment recombines in vivo with plasmid pDONR201 to produce, according to Gateway terminology, an "input clone". Plasmid pDONR2Q1 was purchased from Invitrogen as part of Gateway® technology.

Example 27: Vector Construction

The input clone was subsequently used in an LR reaction with a destination vector used for Oryza sativa transformation. This vector contains as functional elements within the limits of T-DNA: a selectable plant marker; a traceable marker expression drawer; and a Gateway drawer intended for in vivo LR recombination with the sequence of interest already cloned into the input clone. A rice OSH1 promoter (SEQ ID NO: 151) for expression in shoot apical meristem was located prior to this Gateway drawer.

Following the LR recombination step, the resulting expression vector shown in Figure 18 was transformed into Agrobacterium LBA4044 and subsequently to Oryza sativa plants. Transformed rice plants were allowed to grow and were then evaluated for the parameters described in Examples 28 and 29. For transformation of other crops see Example 40.

Example 28: Description of the phenotypic assessment procedure

Approximately 15 to 20 independent T0 rice transformants were generated. Primary transformants were transferred from a tissue crop camera to a greenhouse for T1 seed growth and harvest. Five events, of which T1 progeny segregated 3: 1 for presence / absence of transgene, were retained. For each of these events, approximately 10 T1 seedlings containing the transgene (hetero and homozygotes) and approximately 10 T1 seedlings lacking the transgene (nullizigotes) were selected by monitoring visual marker expression. Suitable transgenic plants and control plants were grown side by side at random positions. From the sowing stage to the maturity stage the plants were passed several times through a digital imaging booth. At each moment digital images (2048x1536 pixels, 16 million colors) were taken from each plant from at least 6 different angles.

Three of the events evaluated in T1 were additionally evaluated in generation T2 following the same evaluation procedure as for generation T1 but with more individuals per event. Seed related parameter measurements

The mature primary panicles were collected, counted, bagged, barcoded and then dried for three days in an oven at 37 ° C. The panicles were then traced and all seeds were collected and counted. The full shells were separated from the empty shells using an air blowing device. The empty shells were discarded and the remaining fraction counted again. The full shells were weighed on an analytical balance. The number of full grains was determined by counting the number of full husks that remained after the separation step. Total seed yield was measured by weighing all full bark collected from a plant. Total number of seeds per plant was measured by counting the number of bark collected from a plant. Thousand Seed Weight (TKW) is extrapolated from the number of full grains counted and their total weight. The harvest index (HI) in the present invention is defined as the ratio of total seed yield to shoot (mm2) multiplied by a factor 106. The total number of flowers per panicle as defined in the present invention is the ratio of the total number of seeds to the number of mature primary panicles. The seed fill rate as defined in the present invention is the ratio (expressed as 25 µm) of the number of full grains to the total number of seeds (or rapiers).

Statistical analysis: F test

A two-way ANOVA (variant analysis) was used as a statistical model for the general evaluation of plant phenotypic characteristics. An F test was performed on all measured parameters of all plants of all events transformed with the gene of the present invention. The F test was performed to check for a gene effect on all transformation events and for a general gene effect, also known as a global gene effect. The threshold for significance for a true global gene effect has been adjusted to a 5% probability level for the F test. A significant F test value points to a gene effect, meaning that it is not just the presence or position of the gene that is present. causing differences in phenotype.

Example 29: Results of the evaluation of transgenic rice plants expressing a modified Arabidopsis thaliana CDC27 nucleic acid under the control of a sprout apical meristem promoter

Evaluation measurement results (seed yield, full grain number, and HI) for transgenic plants expressing a modified CDC27 nucleic acid under the control of a bud apical meristem promoter (OSH1) are shown in Tables T to V. The number of events with an increase, the% difference with appropriate control plants, as well as the ρ values of the F test for T1 and T2 generations are indicated.

Table T: Results of seed yield measurement of transgenic plants expressing a modified CDC27 nucleic acid under the control of a bud apical meristem promoter.

<table> table see original document page 230 </column> </row> <table> Table U: Results of measurement of full grain number of transgenic plants expressing a modified CDC27 nucleic acid under the control of an apical meristem promoter of bud.

<table> table see original document page 231 </column> </row> <table>

Table V: Harvest index measurement results of transgenic plants expressing a modified CDC27 nucleic acid under the control of a bud apical meristem promoter.

<table> table see original document page 231 </column> </row> <table>

Transgenic rice plants expressing a modified CDC27 nucleic acid under the control of shoot apical meristem promoter had significantly increased seed production, increased number of full seeds and increased harvest index.

EXAMPLE E: AT-hook

Example 30: Cloning of Oryza sativa AT-hook encoding nucleic acid gene

The Oryza sativa gene encoding a polypeptide comprising an AT-hook domain and a DUF296 domain (see SEQ ID NO: 152) was PCR amplified using an Oryza sativa seedling cDNA library (Invitrogen, Paisley, UK) as a template. After reverse transcription of seedling-extracted RNA, cDNAs were cloned into pCMV Sport 6.0. Average bank insert size was 1.6 kb and the original number of clones was on the order of 1.67x107 cfu. Original titer was determined to be 3.34 χIO6 cfü / ml after first amplification of 6x1010 cfu / ml. After plasmid extraction, 200 ng of template were used in a 50 μΐ PCR mix. Primers (SEQ ID NO: 196; sense, initiator AttB 1: 5'-ggggacaagtttgtacaaaaaagcaggcttaaacaatggat ccggtcacgg -3 ') and (SEQ ID NO: 197; reverse, complementary, primer AttB2: 5'-ggggaccactaggcagagcagagcagagcagagcagagctag AttB sites for Gateway recombination were used for PCR amplification. PCR was performed using Hifi Taq DNA polymerase under standard conditions. A PCR fragment (including attB sites; from start to finish) was amplified and purified using standard methods. The first step of the Gateway procedure, the BP reaction, was then performed, during which the PCR fragment recombined in vivo with plasmid pDC) NR201 to produce, according to Gateway terminology, an "input clone". Plasmid pDC) NR201 was purchased from Invitrogen as part of Gateway® technology.

Example 31: Vector Construction

The input clone was subsequently used in an LR reaction with a destination vector containing the prolamine promoter used for Oryza sativa transformation. This vector contains as functional elements within the limits of T-DNA: a selectable plant marker; a traceable marker expression drawer; and a Gateway drawer intended for in vivo LR recombination with the sequence of interest already cloned into the input clone. A rice prolamine promoter (SEQ ID NO: 195) for specific endosperm expression was located prior to this Gateway drawer.

Following the LR recombination step, the resulting expression vector shown in Figure 22 was transformed into Agrobacterium LBA4044 and subsequently to Oryza sativa plants. Transformed rice plants were allowed to grow and were then evaluated for the parameters described below. For transformation of other crops see Example 40.

Example 32: Evaluation and Results

Approximately 15 to 20 independent TO transformants of rice were generated. Primary transformants were transferred from a tissue crop camera to a greenhouse for T1 seed growth and harvest. Seven events, of which T1 progeny segregated 3: 1 for presence / absence of transgene, were retained. For each of these events, approximately 10 T1 seedlings containing the transgene (hetero and homozygotes) and approximately 10 T1 seedlings lacking the transgene (nullizigotes) were selected by monitoring visual marker expression.

32.1 Statistical analysis: F test

A two-way ANOVA (variant analysis) was used as a statistical model for the general evaluation of plant phenotypic characteristics. An F test was performed on all measured parameters of all plants of all events transformed with the gene of the present invention. The F test was performed to check for a gene effect on all transformation events and for a general gene effect (also referred to as the overall gene effect). The threshold for significance for a true global gene effect has been adjusted to a 5% probability level for the F test. A significant F test value points to a gene effect, meaning that it is not just the presence or position of the gene that is present. causing differences in phenotype.

32.2 Measurements of seed related parameters

The mature primary panicles were collected, counted, bagged, barcoded and then dried for three days in an oven at 37 ° C. The panicles were then traced and all seeds were collected and counted. The full shells were separated from the empty shells using an air blowing device. The empty shells were discarded and the remaining fraction counted again. The full shells were weighed on an analytical balance. The number of full kernels was determined by counting ρ number of full shells remaining after the separation step. Total seed yield was measured by weighing all full bark collected from a plant. Total number of seeds per plant was measured by counting the number of bark collected from a plant. Thousand Seed Weight (TKW) was extrapolated from the number of full grains counted and their total weight. The harvest index (HI) was expressed as a ratio between total seed production and shoot (mm2), multiplied by a factor of 106. The total number of flowers per panicle was expressed as a ratio between the total number of seeds and the number of mature primary panicles. Seed fill rate was expressed as a% of the number of full grains over the total number of seeds (or rapiers).

Table W: Comparative data to show the difference in seed yield obtained using a specific endosperm promoter (prolamine) compared to a specific root promoter (RCc3 promoter)

<table> table see original document page 234 </column> </row> <table>

The Table shows the% difference in various parameters for transgenic plants compared to corresponding control plants (nullizigotes); Also shown in the Table is the value of ρ from the F test that indicates the overall effect of the gene. As shown in the Table, various seed yield parameters were increased in plants expressing a nucleic acid encoding AT-hook (SEQ ID NO: 152) under the control of a specific endosperm promoter, whereas no increase (indeed a significant decrease). ) was obtained for plants expressing the same transgene under the control of a specific root promoter in transgenic plants. EXAMPLE F: DOF Transcription Factors

Example 33: Arabidopsis thaliana DOF Transcription Factor Gene Cloning (SEQID NO: 198)

The Arabidopsis thaliana DOF transcription factor gene was PCR amplified using a template of an Arabidopsis thaliana seedling cDNA library (Invitrogen, Paisley, UK). After reverse transcription of seedling-extracted RNA, cDNAs were cloned into pCMV Sport 6.0. Average bank insert size was 1.5 kb and original number of clones was 1.59x10 ^ 7 cfu. Original titer was determined to be 9.6x10 ^ 5 cfu / ml after first amplification of 6x10 ^ 11 cfu / ml. After plasmid extraction, 200 ng of template were used in a 50 μl PCR mix. Initiator (SEQ ID NO: 223) (initiator sense AttB 1: 5 'ggggacaagtttgtacaaaaaa gcaggcttaaacaatgggtggatcgatggc 3') and (SEQ ID NO: 224) (initiator AttB2 complementary reverse: 5 'ggggaccgggacgggacaggacaggacggacaggacggacaggacggacaggacgggacaaca Gateway, were used for PCR amplification. PCR was performed using Hifi Taq DNA polymerase under standard conditions. A PCR fragment (including attB sites; from start to finish) was amplified and purified using standard methods as well. The first step of the Gateway procedure, the BP reaction, was then performed during which the PCR fragment recombined in vivo with plasmid pDONR201 to produce, according to Gateway terminology, an "input clone". Plasmid pDONR201 was purchased from Invitrogen as part of Gateway® technology.

Example 33a: Vector Construction

The input clone was subsequently used in an LR reaction with a GOS2 containing destination vector used for Oryza sativa transformation. This vector contained as functional elements within the limits of T-DNA: a selectable plant marker; a traceable marker expression drawer; and a Gateway drawer intended for in vivo LR recombination with the sequence of interest already cloned into the input clone. A rice GOS2 promoter (SEQ ID NO: 225) for constitutive expression was located prior to this Gateway drawer.

Following the LR recombination step, the resulting expression vector shown in Figure 26 was transformed into Agrobacterium LBA4044 and subsequently to Oryza sativa plants. Transformed rice plants were allowed to grow and were then evaluated for the parameters described below. For transformation of other crops see Example 40.

Example 34: Arabidopsis thaliana DOF Transcription Factor Gene Cloning (SEQ ID NO: 226)

The Arabidopsis thaliana DOF transcription factor gene was PCR amplified using a template of an Arabidopsis thaliana seedling cDNA library (Invitrogen, Paisley, UK). After reverse transcription of seedling-extracted RNA, cDNAs were cloned into pCMV Sport 6.0. Average bank insert size was 1.5 kb and original number of clones was 1.59x10 cfu. Original titer was determined to be 9.6x105 cf / ml after first amplification of 6x1011 cfu / ml. After plasmid extraction, 200 ng of template were used in a 50 μΐ PCR mix. Initiator (SEQ ID NO: 256) (AttBl initiator sense: 5 'ggggacaagtttgtacaaaaaa gcaggcttaaacaatgatgatggagagagagagcc3') and (SEQ ID NO: 257) (AttB2 complementary initiator reverse: 5 'ggggaccactgatgatgatataggatataggatacgatacgatacgatacgatacgataccggtacgatacggtacgatacgtaggcagtaggtaggctagggtaggctaggtaggtaggtaggctaggtaggtaggctggtaggtaggtaggtaggagacctggtacgatgatagtgtcggtgtggtgactacgatgtgtatagggtataggctggiggtggiggtcgiggtactgtg name given in the full version) used for PCR amplification. PCR was performed using Hifi Taq DNA polymerase under standard conditions. A PCR fragment (including attB sites; from start to finish) was amplified and purified using standard methods as well. The first step of the Gateway procedure, the BP reaction, was then performed during which the PCR fragment recombined in vivo with plasmid pDC> NR201 to produce, according to Gateway terminology, an "input clone". Plasmid pDC) NR201 was purchased from Invitrogen as part of Gateway® technology.

Example 34a: Vector Construction

The input clone was subsequently used in an LR reaction with a prolamine-containing targeting vector used for Oryza sativa transformation. This vector contains as functional elements within the limits of T-DNA: a selectable plant marker; a traceable marker expression drawer; and a Gateway drawer intended for in vivo LR recombination with the sequence of interest already cloned into the input clone. A rice prolamine promoter (SEQ ID NO: 258) for specific seed expression was located before this Gateway drawer.

After the LR recombination step, the resulting expression vector shown in Figure 27 was transformed into Agrobacterium LBA4044 and subsequently to Oryza sativa plants. Transformed rice plants were allowed to grow and were then evaluated for the parameters described below. For transformation of other crops see Example 40.

Example 35: Evaluation and Results

Approximately 15 to 20 independent TO transformants of rice were generated. Primary transformants were transferred from a tissue crop camera to a greenhouse for growing and harvesting TI seed. Seven events, of which progeny T1 secreted 3: 1 for presence / absence of transgene, were retained. For each of these events, approximately 10 Tl seedlings containing the transgene (hetero and homozygotes) and approximately 10 Tl seedlings lacking the transgene (nullizigotes) were selected by monitoring visual marker expression. Approximately 4 T1 events were additionally evaluated in generation T2 following the same evaluation procedure as for generation T1 but with more individuals per event.

Plants of five events were grown under normal conditions until the spike stage. Soil moisture was continuously monitored using moisture sensors inserted into the pots of several randomly selected non-transgenic control plants. In a first phase, the pots were saturated to a maximum of 60% to reduce pot-to-pot variability. Once the pots were saturated, irrigation was retained until a soil moisture content of below 20% was obtained. The plants were then re-irrigated until soil moisture reached the maximum level of 60% again. Plants were then represented to evaluate the following root-related and seed-related parameters.

Root Related Parameters

Plants were grown in specially designed pots with transparent backgrounds to allow root visualization. A digital camera recorded images across the bottom of the pot during plant growth. Root characteristics such as total projected area (which can be correlated with total root volume), root diameter and root length above a certain threshold of thickness (thick root length, or thin root length) were deduced from generated image using appropriate computational application.

Seed related parameter measurements

The mature primary panicles were collected, counted, bagged, barcoded and then dried for three days in an oven at 37 ° C. The panicles were then traced and all seeds were collected and counted. The full shells were separated from the empty shells using an air blowing device. The empty shells were discarded and the remaining fraction counted again. The full shells were weighed on an analytical balance. The number of full grains was determined by counting the number of full husks that remained after the separation step. Total seed yield was measured by weighing all full bark collected from a plant. Total number of seeds per plant was measured by counting the number of bark collected from a plant. Thousand Seed Weight (TKW) was extrapolated from the number of full grains counted and their total weight. The harvest index (HI) in the present invention is defined as the ratio of total seed yield to shoot (mm2) multiplied by a factor 106. The total number of flowers per panicle as defined in the present invention is the ratio of the total number of seeds to the number of mature primary panicles. The seed fill rate as defined in the present invention is the ratio (expressed as a%) of the number of full grains to the total number of seeds (or rapiers).

Statistical analysis: F test

A two-way ANOVA (variant analysis) was used as a statistical model for the general evaluation of plant phenotypic characteristics. An F test was performed on all measured parameters of all plants of all events transformed with the gene of the present invention. The F test was performed to check for a gene effect on all transformation events and for a general gene effect, also known as a global gene effect. The threshold for significance for a true global gene effect has been adjusted to a 5% probability level for the F test. A significant F test value points to a gene effect, meaning that it is not just the presence or position of the gene that is present. causing differences in phenotype.

Table X below shows the results of T2 evaluation for transgenic plants expressing a nucleic acid encoding a DOF transcription factor under the control of a GOS2 promoter and the results of T2 evaluation for transgenic plants expressing a nucleic acid encoding a DOF transcription factor under the control of a prolamine promoter. Although not shown, comparable results were obtained for Tl plants. The ρ value of the F test is shown for the parameters listed in the Table, as well as the percentage difference between transgenic plants versus nullizigotes.

Table X: T2 Evaluation Results

<table> table see original document page 240 </column> </row> <table>

In addition to the seed-related parameters described above, the following root parameters were also increased in transgenic plants compared to nullizigotes: 14% increase in total root biomass, 7% increase in number of thick roots (internal threshold), 36 % increase in number of thin roots (internal threshold) and an 8% increase in mean root diameter.

The results mentioned above were obtained under mild drought stress conditions; Similar results would be expected under normal or stress-free conditions.

EXAMPLE G: CKI

Example 36: Cloning of an Oryza sativa Gene Encoding a CKI4 Polypeptide

The Oryza sativa gene encoding a CKI4 polypeptide was PCR amplified using an Oryza sativa cell suspension cDNA library cloned into the HybriZAP-2.1 kit vector pAD-Gal4-2.1 (Stratagene, La Jolla, California USA). according to the manufacturer's instructions. Average bank insert size was 1.5 kb and the original number of clones was around 2x106 pfu. Original titer was determined to be 4x106 pfu / ml and after the first amplification of 1010 pfu / ml. After plasmid extraction, 200 ng of template were used in a 50 μl PCR mix. Primers (SEQ ID NO: 284; sense, bold start codon, AttBl site in italics: 5'- GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACAATGGGCAAGTACA TGCGCAAGGCC-3 ') and (SEQ ID NO: 285; reverse 2, complementary'-Att GGGGACCACTTTGTACAAGAAAGCTGGG 7GGAGCAGAGAGGTCCATGGTGCCC-3 '), which includes AttB sites for Gateway recombination, were used for PCR amplification. PCR was performed using Hifi Taq DNA polymerase under standard conditions. A 662 bp PCR fragment (including attB; 585 bp start-to-end sites) was amplified and purified using standard methods as well. The first step of the Gateway procedure, the BP reaction, was then performed, during which the PCR fragment recombines in vivo with plasmid pDONR201 to produce, according to Gateway terminology, an "input clone". Plasmid pDONR201 was purchased from Invitrogen as part of Gateway® technology.

Example 3 7: Vector Construction

The input clone was subsequently used in an LR reaction with a destination vector used for Oryza sativa transformation. This vector contains as functional elements within the limits of T-DNA: a selectable plant marker; a traceable marker expression drawer; and two opposite orientation Gateway drawers intended for in vivo LR recombination with the sequence of interest already cloned into the input clone. The two Gateway drawers were separated by non-coding DNA (in this case a 315 bp fragment from a tobacco matrix coupling region (MAR), reference NCBI U67919, fragment 774 to 1088 bp), to promote formation of a structure in clip form of mRNA after transcription. A rice RP6 prolamine promoter (SEQ ID NO: 281) for specific endosperm expression was located before the first Gateway drawer, in opposite orientation with respect to the promoter.

The input clone was also used in an LR reaction with another target vector used for Oryza sativa transformation. This vector was identical to that described above except that the RP6 prolamine promoter was replaced by rice beta-expansin promoter of SEQ ID NO: 282.

After the LR recombination step, the two resulting expression vectors (Figure 32 for both vectors) were transformed into Agrobacterium LBA4044 and subsequently to Oryza sativa plants. Transformed rice plants were allowed to grow and were then evaluated for the parameters described in Examples 38 and 39. For transformation of other crops see Example 40.

Example 38: Description of the phenotypic assessment procedure

Approximately 15 to 20 independent TO transformants of rice were generated. Primary transformants were transferred from a tissue crop camera to a greenhouse for T1 seed growth and harvest. Four to five events, of which T1 progeny segregated 3: 1 for transgene presence / absence, were retained. For each of these events, approximately 10 T1 seedlings containing the transgene (hetero and homozygous) and approximately 10 T1 seedlings lacking the transgene (nullizigotes) were selected by monitoring visual marker expression. Suitable transgenic plants and control plants were grown side by side at random positions. From the sowing stage to the maturity stage the plants were passed several times through a digital imaging booth. At each moment digital images (2048x1536 pixels, 16 million colors) were taken from each plant from at least 6 different angles.

The same events evaluated at T1 were additionally evaluated at T2 generation following the same evaluation procedure as for T1 generation.

Seed related parameter measurements

The mature primary panicles were collected, counted, bagged, barcoded and then dried for three days in an oven at 37 ° C. The panicles were then traced and all seeds were collected and counted. The full shells were separated from the empty shells using an air blowing device. The empty shells were discarded and the remaining fraction counted again. The full shells were weighed on an analytical balance. The number of full grains was determined by counting the number of full husks that remained after the separation step. Total seed yield was measured by weighing all full bark collected from a plant. Total number of seeds per plant was measured by counting the number of bark collected from a plant. The harvest index (HI) in the present invention is defined as the ratio of total seed production to shoot (mm2) multiplied by a factor 10 ^ 6. The total number of flowers per panicle as defined in the present invention is the ratio of the total number of seeds to the number of mature primary panicles. The seed fill rate as defined in the present invention is the ratio (expressed as a%) of the number of full grains to the total number of seeds (or rapiers).

Statistical analysis: F test

A two-way ANOVA (variant analysis) was used as a statistical model for the general evaluation of plant phenotypic characteristics. An F test was performed on all measured parameters of all plants of all events transformed with the gene of the present invention. The F test was performed to check for a gene effect on all transformation events and for a general gene effect, also known as a global gene effect. The threshold for significance for a true global gene effect has been adjusted to a 5% probability level for the F test. A significant F test value points to a gene effect, meaning that it is not just the presence or position of the gene that is present. causing differences in phenotype.

Example 39: Results of evaluation of transgenic rice plant with reduced CKI4 expression in endosperm

The evaluation measurement results (seed yield, number of full grains, total number of seeds and flowers per panicle) for transgenic plants with reduced endosperm CKI4 expression are presented in Table Y below. The number of plants with an increase in one parameter, the mean percent increase as well as the P value of the T2 generation are shown, and compared with results obtained with transgenic plants with reduced CKI4 expression using a beta expansin promoter for preferential expression in bud tissue.

Results show that reduced CKI4 expression in endosperm yields significantly increased seed weight, full grain number, total number of seeds and flowers per panicle compared to nullizigotes and compared to transgenic plants with preferentially reduced CKI4 expression in tissue. bud (using a beta expansin promoter). Table Y: Evaluation measurement results for transgenic plants with reduced CKI4 expression in endosperm

<table> table see original document page 245 </column> </row> <table>

Example 40: Corn, Wheat, Soybean, Rapeseed and Alfalfa Transformation

Corn Processing

Corn transformation (Zea mays) is performed with a modification of the method described by Ishida et al. (1996) Nature Biotech 14 (6): 745-50. Transformation is genotype dependent in maize and only specific genotypes are receptive to transformation and regeneration. The Al 88 (University of Minnesota) natural line or Al 88 hybrids as an ancestor are good sources of donor material for transformation, but other genotypes can also be used successfully. Ears are harvested from maize plant approximately 11 days after pollination (DAP) when the immature embryo length is about 1 to 1.2 mm. Immature embryos are co-cultured with Agrobacterium tumefaciens containing the expression vector, and transgenic plants are recovered by organogenesis. Excised embryos are grown in callus induction medium, then corn regeneration medium, containing the selection agent (eg imidazolinone but various selection markers may be used). Petri dishes are incubated in light at 25 ° C for 2-3 weeks, or until buds develop. Green shoots are transferred from each embryo to maize rooting medium and incubated at 25 ° C for 2-3 weeks until roots develop. Rooted shoots are transplanted to soil in the greenhouse. T1 seeds are produced from plants that exhibit selection agent tolerance and contain a single copy of the T-DNA insert.

Wheat processing

Wheat processing is performed with the method described by Ishida et al. (1996) Nature Biotech 14 (6): 745-50. The Bobwhite cultivar (available from CIMMYT, Mexico) is commonly used in processing. Immature embryos are co-cultured with Agrobacterium tumefaciens containing the expression vector, and transgenic plants are recovered by organogenesis. After incubation with Agrobacterium, the embryos are grown in vitro on callus-inducing medium, then regenerating medium, containing the selection agent (eg imidazolinone but various selection markers may be used). Petri dishes are incubated in light at 25 ° C for 2-3 weeks, or until buds develop. Green shoots are transferred from each embryo to rooting medium and incubated at 25 ° C for 2-3 weeks until roots develop. Rooted shoots are transplanted to soil in the greenhouse. T1 seeds are produced from plants which exhibit tolerance to the selection agent and which contain a single copy of the T-DNA insert.

Soybean Processing

Soybean is transformed according to a modification of the method described in Texas A&M Patent 5,164,310. Several commercial soybean varieties are receptive to processing by this method. The Jack cultivar (available from the Illinois Seed foundation) is commonly used for processing. Soybean seeds are sterilized for in vitro sowing. The hypocotyl, radicle and cotyledon are excised from young seven-day-old seedlings. The epicotyl and the remaining cotyledon are further grown to develop axillary nodes. These axillary nodes are excised and incubated with Agrobacterium tumefaciens containing the expression vector. After the co-cultivation treatment, the explants are washed and transferred to selection medium. Regenerated shoots are excised and placed in a shoot extension medium. Sprouts smaller than 1 cm are placed in rooting medium until roots develop. Rooted shoots are transplanted to soil in the greenhouse. T1 seeds are produced from plants which exhibit tolerance to the selection agent and which contain a single copy of the T-DNA insert.

Rapeseed / canola transformation

Cotyledonary and hypocotyl petioles of 5-6 day old seedlings are used as tissue harvest explants and transformed according to Babic et al. (1998, Plant Cell Rep 17: 183-188). The commercial cultivar Westar (Agriculture Canada) is the standard variety used for processing, but other varieties can also be used. Canola seeds are surface-sterilized for in vitro sowing. Cotyledon petiole explants with coupled cotyledon are excised from seedlings in vitro, and inoculated with Agrobacterium (containing the expression vector) by bathing the cutting end of the petiole explant in the bacterial suspension. The explants are then cultured for 2 days in MSBAP-3 medium containing 3 mg / l BAP, 3% sucrose, 0.7% pH ytagar at 23 ° C, 16 h light. After two days of co-cultivation with Agrobacterium, petiole explants are transferred to MSBAP-3 medium containing 3 mg / l BAP, cefotaxime, carbenicillin, or timentin (300 mg / l) for 7 days, and then grown in MSBAP-3 medium with cefotaxime, carbenicillin, or timentin and selection agent until shoot regeneration. When the shoots are 5-10 mm long, they are cut and transferred to shoot extension medium (MSBAP-0.5 containing 0.5 mg / l BAP). Sprouts about 2 cm long are transferred to rooting medium (MSO) for root induction. Rooted shoots are transplanted to soil in the greenhouse. T1 seeds are produced from plants that exhibit selection agent tolerance and contain a single copy of the T-DNA insert.

Alfalfa Transformation

A regenerating alfalfa clone (Medicago sativa) is transformed using the method of (McKersie et al., 1999 PIant physiol 119: 839-847). Alfalfa regeneration and transformation is genotype dependent and therefore a regenerating plant is required. Methods to obtain regenerating plants have been described. For example, they may be selected from the cultivar Rangelander (Agriculture Canada) or any other commercial alfalfa variety as described by Brown DCW and Atanassov (1985. Plant Cell Tissue Organ Culture 4: 111-112). Alternatively, the RA3 variety (University of Wisconsin) has been selected for use in tissue harvesting (Walker et al., 1978 Am J Bot 65: 654-659). Petiole explants are co-cultured with a nocturnal crop of Agrobacterium tumefaeiens C58C1 pMP90 (McKersie et al., 1999 Plant physiol 119: 839-847) or LBA4404 containing the expression vector. Explants are co-cultured for 3 d in the dark in SH induction medium containing 288 mg / l Pro, 53 mg / l thioproline, 4.35 g / l K2SO4, and 100 μm acetoseringone. The explants are washed in half-strength Murashige-Skoog medium (Murashige and Skoog, 1962) and placed in the same SH induction medium without acetoseringone but with a suitable selection agent and suitable antibiotic to inhibit Agrobaeterium growth. After several weeks, somatic embryos are transferred to BOY2 development medium containing no growth regulator, no antibiotic, and 50 g / L sucrose. Somatic embryos are subsequently germinated in Murashige-Skoog medium with half force. Rooted seedlings were transplanted into pots and grown in a greenhouse. T1 seeds are produced from plants that exhibit selection agent tolerance and contain a single copy of the T-DNA insert. SEO LISTING

<110> CropDesign N.V.

<120> "PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND METHODS FOR MAKING THE SAME" PF57958 EP 05111597.0 2005-12-01 US 60 / 742,352 2005-12-05 EP 05111691.1 2005-12-05 EP 05111786.9 2005-12-07 <150 > US 60 / 748,903 <151> 2005-12-08 <150> US 60 / 749,219 2005-12-09 EP 05111996.4 2005-12-12 <150> US 60 / 750,143 <151> 2005-12-14 <150> EP 05112562.3 2005-12-21 US 60 / 753,650 2005-12-23 EP 05113110.0 2005-12-30 EP 05113111.8

2005-12-30 US 60 / 756,086

2006-01-04 <150> US 60 / 756,042 <151> 2006-01-04

285

PatentIn version 3.3 1

353 DNA

<213> Oryza sativa <4 00> 1

atggaaggtg taggtgctag gcagaggagg aaccctctga tacccagacc aaacggttca 60

aagaggcatc tgcagcatca gcatcagcca aatgctgccg agaagaagac cgccgcgaca 120 tcgaattact tcagtatcga ggcgttcctc gtgctcgtct tcctcaccat gtcattgctc 180 atacttccat tggtgcttcc cccattgcct ccgccgccat cgctgctgct gctgctgcca 240 gtctgcctgc tcatcctgct ggttgtgctg gccttcatgc caacggatgt 300 gcggagcatg

<130> <150> <151> <150> <151> <150> <151> <150> <151>

<151> <150> <151>

<151> <150> <151> <150> <151> <150> <151> <150> <151>

<160> <17 0> <210> <211> <212>

gcttcctctt acttgtaaat acatctccta ggggaattta tttttgtttt tga <210> 2 <211> 105 <212> PRT

<213> Oryza sativa <400> 2

Met Glu Gly Val Gly Arg Wing Arg Gln Arg Arg Asn Pro Read Ile Pro Arg 1 5 10 15

Pro Asn Gly Be Lys Arg His Leu Gln His Gln His Gln Pro Asn Ala

20 25 30

Glu Wing Lys Lys Thr Wing Wing Thr Be Asn Tyr Phe Ser Ile Glu Wing

35 40 45

Phe Leu Val Leu Val Phe Leu Thr Met Ser Leu Leu Ile Leu Pro Leu

50 55 60

Val Leu Pro Pro Leu Pro Pro Pro Ser Leu Leu Leu Leu Pro 65 70 75 80

353 Val Cys Leu Leu Ile Leu Leu Val Val Leu Wing Phe Met Pro Thr Asp

85 90 95

Val Arg Be Met Wing Be Ser Tyr Leu 100 105

<210> 3 <211> 56 <212> DNA

<213> Artificial sequence <220>

<223> initiator: prm08170 <400> 3

ggggacaagt ttgtacaaaa aagcaggctt aaacaatgga aggtgtaggt gctagg 56

<210> 4 <211> 51 <212> DNA

<213> Artificial sequence <220>

<223> initiator: prm08171 <400> 4

ggggaccact ttgtacaaga aagctgggtc aaaaacaaaa ataaattccc c 51

<210> 5

<211> 2193

<212> DNA

<213> Oryza sativa

<400> 5

aatccgaaaa gtttctgcac cgttttcacc ccctaactaa caatataggg aacgtgtgct 60

aaatataaaa tgagacctta tatatgtagc gctgataact agaactatgc aagaaaaact 120 catccaccta ctttagtggc aatcgggcta aataaaaaag agtcgctaca ctagtttcgt 180 tttccttagt aattaagtgg gaaaatgaaa tcattattgc ttagaatata cgttcacatc 240 tctgtcatga agttaaatta ttcgaggtag ccataattgt catcaaactc ttcttgaata 300 aaaaaatctt tctagctgaa ctcaatgggt aaagagagag atttttttta aaaaaataga 360 atgaagatat tctgaacgta ttggcaaaga tttaaacata taattatata attttatagt 420 ttgtgcattc gtcatatcgc acatcattaa ggacatgtct tactccatcc caatttttat 480 ttagtaatta aagacaattg acttattttt attatttatc ttttttcgat tagatgcaag 540 gtacttacgc acacactttg tgctcatgtg catgtgtgag tgcacctcct caatacacgt 600 tcaactagca acacatctct aatatcactc gcctatttaa tacatttagg tagcaatatc 660 tgaattcaag cactccacca tcaccagacc acttttaata atatctaaaa tacaaaaaat 720 aattttacag aatagcatga aaagtatgaa acgaactatt taggtttttc acatacaaaa 780 aaaaaaagaa ttttgctcgt gcgcgagcgc caatctccca tattgggcac acaggcaaca 840 acagagtggc tgcccacaga acaacccaca aaaaacgatg atctaacgga ggacagcaag 900 tccgcaacaa ccttttaac the gcaggctttg cggccaggag agaggaggag aggcaaagaa 960 aaccaagcat cctcctcctc ccatctataa attcctcccc ccttttcccc tctctatata 1020 ggaggcatcc aagccaagaa gagggagagc accaaggaca cgcgactagc agaagccgag 1080 cgaccgcctt cttcgatcca tatcttccgg tcgagttctt ggtcgatctc ttccctcctc 1140 cacctcctcc tcacagggta tgtgcccttc ggttgttctt ggatttattg ttctaggttg 1200 tgtagtacgg gcgttgatgt taggaaaggg gatctgtatc tgtgatgatt cctgttcttg 1260 gatttgggat agaggggttc ttgatgttgc atgttatcgg ttcggtttga ttagtagtat 1320 ggttttcaat cgtctggaga gctctatgga aatgaaatgg tttagggtac ggaatcttgc 1380 gattttgtga gtaccttttg tttgaggtaa aatcagagca ccggtgattt tgcttggtgt 1440 aataaaagta cggttgtttg gtcctcgatt ctggtagtga tgcttctcga tttgacgaag 1500 ctatcctttg tttattccct attgaacaaa aataatccaa ctttgaagac ggtcccgttg 1560 atgagattga atgattgatt cttaagcctg tccaaaattt cgcagctggc ttgtttagat 1620 acagtagtcc ccatcacgaa attcatggaa acagttataa tcctcaggaa caggggattc 1680 cctgttcttc cgatttgctt tagtcccaga attttttttc ccaaatatct taaaaagtca 1740 ctttctggtt cagttcaatg aattg attgc tacaaataat gcttttatag cgttatccta 1800 gctgtagttc agttaatagg taatacccct atagtttagt caggagaaga acttatccga 1860 tttctgatct ccatttttaa ttatatgaaa tgaactgtag cataagcagt attcatttgg 1920 attatttttt ttattagctc tcaccccttc attattctga gctgaaagtc tggcatgaac 1980 tgtcctcaat tttgttttca aattcacatc gattatctat gcattatcct cttgtatcta 2040 cctgtagaag tttctttttg gttattcctt gactgcttga ttacagaaag aaatttatga 2100 agctgtaatc gggatagtta tactgcttgt tcttatgatt catttccttt gtgcagttct 2160 tggtgtagct tgccactttc accagcaaag ttc 2193

<210> 6 <211> 3 <212> PRT

<213> Artificial sequence <220>

<223> motif saved Ia <400> 6 Tyr Phe Ser 1

<210> 7 <211> 3 <212> PRT

<213> Artificial sequence <220>

<223> conserved motif Ib <400> 7 Tyr Phe Thr 1

<210> 8 <211> 3 <212> PRT

<213> Artificial sequence <220>

<223> conserved motif Ic <400> 8 Tyr Phe Gly 1

<210> 9 <211> 3 <212> PRT

<213> Artificial sequence <220>

<223> motif retained Id <400> 9 Tyr Leu Gly 1

<210> 10 <211> 7 <212> PRT

<213> Artificial sequence <220>

<223> Subject retained 2 <220>

<221> VARIANT <222> (1) .. (1)

<223> / replace = "Wing" / replace = "Ile" <220>

<221> VARIANT

<222> (7) .. (7)

<223> / replace = "Be"

<400> 10

Val Leu Wing Phe Met Pro Thr 1 5

<210> 11 <211> 3 <212> PRT

<213> Artificial sequence <220>

<223> Subject retained 3 <220>

<221> VARIANT <222> (1) .. (1) <223> / replace = "Pro" <400> 11 Ser Tyr Leu 1

<210> 12 <211> 178 <212> PRT

<213> Oryza sativa <4 00> 12

Met Tyr Leu Read To Be Pro Arg Asn Gly Asp Glu Glu Asp Glu Gln Glu 1 5 10 15

Glu Ile Gln Glu Leu Ile Ser Asp Asp Glu Pro Pro Asn Leu Lys Leu

20 25 30

Ala Ser Cys Ala Thr Ala Ala Ser Ser Ser Ser Ser Gly Ser Asp

35 40 45

Met Glu Lys Gly Arg Gly Lys Wing Cys Gly Gly Gly Be Thr Wing Pro

50 55 60

Pro Pro Pro Pro Pro Be Ser Gly Lys Ser Gly Gly Gly Gly Gly Gly 65 70 75 80

Be Asn Ile Arg Glu Wing Wing Wing Be Gly Gly Gly Gly Gly Val Trp

85 90 95

Gly Lys Tyr Phe Ser Val Glu Ser Leu Leu Leu Leu Val Cys Val Thr

100 105 110

Wing Ser Leu Val Ile Leu Pro Leu Val Leu Pro Pro Leu Pro Pro Pro

115 120 125

Pro Be Met Leu Met Leu Val Pro Val Wing Met Leu Val Leu Leu Leu

130 135 140

Ala Leu Ala Phe Met Pro Thr Thr Thr Ser Ser Ser Ser Ser Ga 145 150 155 160

Gly Gly Gly Gly Gly Gly Gly Arg Asn Gly Wing Thr Thr Gly His Wing Pro 165 170 175

Tyr leu

<210> 13 <211> 126 <212> PRT

<213> Oryza sativa <4 00> 13

Met Leu Leu Glu His Leu Met Ile Thr Met Glu Glu Gln Met Phe Arg 15 10 15

Glu Gln Gln Met Gln Arg Gly Gly Arg His His

20 25 30

Arg Glu Gln Glu Gln Gln Gln Lys Gln Gln Gln Arg Arg Arg Arg Read Le Met

35 40 45

Asn Asn Ala Thr Asn Gly Gly Gly Gly Asp Gly Gly Being Arg Cys Tyr

50 55 60

Phe Ser Thr Glu Wing Ile Leu Val Leu Val Cys Wing Val Thr Val Ser Leu 65 70 75 80

Leu Val Leu Pro Leu Ile Leu Pro Pro Leu Pro Pro Pro Thr Leu

85 90 95

Leu Leu Leu Leu Pro Val Cys Leu Leu Wing Leu Leu Val Val Leu Wing

100 105 110

Phe Met Pro Thr Asp Met Arg Thr Met Wing Be Ser Tyr Leu 115 120 125

<210> 14 <211> 105 <212> PRT <213> Zea mays <220>

<221> UNSAFE <222> (65) .. (75)

<223> Xaa can be any naturally occurring amino acid <4 00> 14

Met Wing Be Arg Be Be Wing Met Glu Gly Gly Wing Wing Ile Gln Arg 15 10 15 Arg Asn Wing Val Lys Arg His Leu Gln Gln Arg Gln Gln Glu Wing Asp

20 25 30

Phe Le Asp Lys Lys Val Ile Wing Be Thr Tyr Phe Be Ile Gly Wing

35 40 45

Phe Leu Val Leu Cys Wing Leu Thr Val Ser Leu Leu Ile Leu Pro Leu

50 55 60

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu Leu Trp Leu Pro 65 70 75 80

Val Cys Leu Leu Val Leu Leu Val Val Leu Wing Phe Met Pro Thr Asp

85 90 95

Val Arg Be Met Wing Be Ser Tyr Leu 100 105

<210> 15 <211> 110 <212> PRT

<213> Triticum aestivum <400> 15

Met Asp Be Gln Phe Gly Wing Read Glu Arg Gly Gly Be Arg Gln Arg 1 5 10 15

Arg Be Pro Val Leu Wing Arg Pro Asn Thr Thr Lys Arg His Ile Gln

20 25 30

Gln Gln Arg Wing Asn Wing Wing Asp Lys Val Val Val Met Asn Tyr

35 40 45

Phe Ser Ile Glu Wing Phe Phe Val Leu Cys Wing Leu Thr Val Ser Leu

50 55 60

Leu Ile Leu Pro Leu Val Leu Pro Pro Leu Pro Pro Pro Pro Leu 65 70 75 80

Leu Leu Phe Val Pro Val Cys Leu Leu Ile Leu Leu Met Val Leu Wing

85 90 95

Phe Met Pro Thr Asp Met Arg Be Met Wing Thr Be Tyr Leu 100 105 110

<210> 16 <211> 110 <212> PRT

<213> Hordeum vulgare <400> 16

Met Asp Be Gln Phe Gly Wing Met Asp Be Gln Phe Gly Arg 15 15 15

Ser Ser Pro Val Leu Arg Wing Pro Asn Thr Wing Lys Arg Gln Met Gln

20 25 30

Gln Gln Arg Wing Asn Wing Wing Asp Wing Val Lys Val Val Ile Pro Asn Tyr

35 40 45

Phe Gly Val Glu Wing Phe Phe Val Leu Cys Wing Leu Thr Val Ser Leu

50 55 60

Leu Ile Leu Pro Leu Val Leu Pro Pro Leu Pro Pro Pro Pro Leu 65 70 75 80

Leu Leu Leu Leu Pro Val Cys Leu Leu Ile Leu Leu Met Val Leu Wing

85 90 95

Phe Met Pro Thr Asp Val Arg Be Met Wing Thr Be Tyr Leu 100 105 110

<210> 17 <211> 99 <212> PRT

<213> Saccharum officinarum <220>

<221> UNSAFE <222> (62) .. (62)

<223> Xaa can be any naturally occurring amino acid <4 00> 17

Met Glu Gly Gly Gly Glly Ile Gln Arg Arg Asn Asn Wing Val Lys Arg 15 10 15

His Leu Gln Gln Arg Gln Gln Glu Wing Asp Phe Leu Asp Lys Lys Val 20 25 30 Ile Wing Be Thr Tyr Phe Be Ile Glu Wing Phe Leu Val Leu Wing Cys

35 40 45

Leu Thr Val Ser Leu Leu Ile Leu Pro Leu Val Leu Pro Xaa Leu Pro

50 55 60

Pro Wing Al Ser Ser Leu Leu Leu Trp Leu Pro Val Trp Leu Leu Glu Leu 65 70 75 80

Leu Ile Val Leu Phe Wing Pro Thr Thr Asp Val Arg Be Met Wing Ser 85 90 95

Ser Tyr Leu

<210> 18 <211> 99 <212> PRT

<213> Saccharum officinarum <4 00> 18

Met Glu Gly Gly Gly Glly Ile Gln Arg Arg Asn Asn Wing Val Lys Arg 1 5 10 15

His Leu Gln Gln Arg Gln Gln Glu Wing Asp Phe Read Asp Lys Lys Val

20 25 30

Ile Wing Be Thr Tyr Phe Be Ile Glu Wing Phe Leu Val Leu Wing Cys

35 40 45

Leu Thr Val Ser Leu Leu Ile Leu Pro Leu Val Leu Pro Pro Leu Pro

50 55 60

Pro Pro Pro Ser Leu Leu Leu Trp Leu Pro Val Cys Leu Leu Ile Leu 65 70 75 80

Leu Ile Val Leu Phe Wing Pro Thr Thr Asp Val Arg Be Met Wing Ser 85 90 95

Ser Tyr Leu

<210> 19 <211> 107 <212> PRT

<213> Sorghum bicolor <4 00> 19

Met Wing Be Arg Be Wing Wing Read Glu Gly Gly Gly Wing Wing Ile Gln 15 10 15

Arg Arg Asn Asn Wing Val Lys Arg His Leu Gln Gln Arg Gln Gln Glu

20 25 30

Wing Asp Phe His Asp Lys Lys Val Ile Wing Be Thr Tyr Phe Ser Ile

35 40 45

Gly Wing Phe Leu Val Leu Cys Wing Leu Thr Phe Ser Leu Leu Ile Leu

50 55 60

Pro Leu Val Leu Pro Pro Leu Pro Pro Pro Pro Ser Leu Leu Leu Trp 65 70 75 80

Leu Pro Val Cys Leu Leu Val Leu Leu Val Val Leu Phe Met Pro Wing

85 90 95

Thr Asp Val Arg Be Val Wing Wing Be Tyr Leu 100 105

<210> 20 <211> 130 <212> PRT

<213> Arabidopsis thaliana <400> 20

Met Ile Arg Glu Ile Ser Asn Leu Gln Lys Asp Ile Ile Asn Ile Gln 15 10 15

Asp Be Tyr Be Asn Asn Arg Val Met Asp Val Gly Arg Asn Asn Arg

20 25 30

Lys Asn Met Be Phe Arg Be Pro Glu Lys Be Lys Gln Glu Leu

35 40 45

Arg Arg Be Phe Be Wing Gln Lys Arg Met Met Ile Pro Wing Asn Tyr

50 55 60

Phe Ser Leu Glu Ser Leu Phe Leu Leu Val Gly Leu Thr Wing Ala Leu 65 70 75 80 Leu Ile Leu Pro Leu Val Leu Pro Pro Leu Pro Pro Phe Met

85 90 95

Leu Leu Leu Val Pro Ile Gly Ile Met Val Leu Leu Val Val Leu Wing

100 105 110

Phe Met Pro To Be His To Be Asn Wing Asn Thr Asp Val Thr Cys Asn 115 120 125

Phe Met 130 <210> 21 <211> 135 <212> PRT

<213> Arabidopsis thaliana <400> 21

Met Ile Arg Glu Phe Ser Be Leu Gln Asn Asp Ile Ile Asn Ile Gln 1 5 10 15

Glu His Tyr Ser Leu Asn Asn Asn Met Asp Val Arg Gly Asp His Asn

20 25 30

Arg Lys Asn Thr Be Phe Arg Gly Be Ala Pro Ala Pro Ile Met Gly

35 40 45

Lys Gln Glu Read Phe Arg Thr Read It Be Ser Gln Asn Be Pro Arg Arg

50 55 60

Leu Ile Ser Ala Ser Tyr Phe Ser Leu Glu Ser Met Val Val Leu Val 65 70 75 80

Gly Leu Thr Wing Be Leu Leu Ile Leu Pro Leu Ile Leu Pro Pro Leu

85 90 95

Pro Pro Pro Pro Phe Met Leu Leu Leu Ile Pro Ile Gly Ile Met Val

100 105 110

Leu Leu Met Val Leu Wing Phe Met Pro Be Ser Asn Ser Lys His Val

115 120 125

Being Being Being Being Thr Phe Met 130 135

<210> 22 <211> 139 <212> PRT

<213> Vitis vinifera <400> 22

Met Ser Ile Glu Gln Pro Glu Wing Asp Be Arg Read Le Be Glu Gly Pro 1 5 10 15

Leu Ile Asn Leu Gln Asp Arg Tyr Leu Ser Gly Ile Met Glu Ala Arg

20 25 30

Gly Arg Arg Asn Be Pro Wing Read Gln Val Glu Arg Lys Asn Pro Thr

35 40 45

Pro Pro Met Glu Wing Gly Lys Lys Met Glu Tyr Asn Arg Thr Pro Leu

50 55 60

Ser Arg Glu Asn Ser Arg Arg Leu Ile Pro Wing Ser Tyr Phe Ser Leu 65 70 75 80

Glu Ser Leu Leu Leu Leu Ile Cys Leu Thr Ala Ser Leu Leu Ile Leu

85 90 95

Pro Leu Ile Leu Pro Pro Leu Pro Pro Pro Phe Met Leu Leu Leu

100 105 110

Leu Pro Ile Gly Ile Leu Val Wing Leu Met Ile Leu Phe Met Pro Wing

115 120 125

Ser Asn Val Arg Asp Read Thr Tyr Thr Tyr Val

130 135

<210> 23 <211> 126 <212> PRT <213> Citrus sp. <220>

<221> UNSAFE <222> (103) .. (103)

<223> Xaa can be any naturally occurring amino acid <400> 23 Met Asn Ser Asp Asn Ser Glu Ser Arg Gln Arg Leu Ser Lys Gly Ile 15 10 15

Ile Asn Read Gln Asp Arg Tyr Pro Thr Be Ile Met Asp Arg Gly Val

20 25 30

Arg Lys Ile Wing Pro Pro Val Glu Lys Arg Lys Val Glu Tyr His

35 40 45

Arg Be Tyr Be Gln Gly Wing Be Arg Lys Read Phe Be Wing Be Tyr

50 55 60

Phe Thr Leu Glu Ser Leu Leu Leu Leu Val Cys Leu Thr Ala Ser Leu 65 70 75 80

Leu Ile Leu Pro Leu Val Leu Pro Pro Leu Pro Pro Pro Phe Leu

85 90 95

Leu Leu Leu Val Pro Ile Xaa Ile Leu Wing Val Leu Leu Val Leu Wing

100 105 110

Phe Met Pro Being Asn Val Arg Asp Ile Thr Being Thr Tyr Val 115 120 125

<210> 24 <211> 118 <212> PRT

<213> Lycorpersicon esculentum <400> 24

Met Asn Met Asp Met Glu Be Glu Ala Lys Leu Arg Be Ser Lys 15 10 15

Gly Phe Asle Asn Leu Glu Glu His Gln Tyr Phe Asn Asn Ile Met

20 25 30

Glu Gly Asn Lys Met Glu His Lys Arg Be Phe Thr Gln Gly His Gly

35 40 45

Lys Lys Met Leu Be Met Asn Tyr Phe Be Leu Glu Be Ile Ile Leu

50 55 60

Leu Leu Gly Leu Thr Wing Ser Leu Leu Leu Leu Pro Leu Met Leu Pro 65 70 75 80

Pro Leu Pro Pro Pro Phe Met Leu Leu Leu Val Pro Ile Phe Ile

85 90 95

Leu Val Val Leu Met Ile Leu Wing Phe Met Pro Ser Asn Val Arg Asn

100 105 110

Val Thr Cys Ser Tyr Leu 115

<210> 25 <211> 87 <212> PRT

<213> Lycorpersicon esculentum <400> 25

Met Glu Gly Asn Lys Met Glu His Lys Arg Be Phe Thr Gln Gly His 15 10 15

Gly Lys Lys Met Leu Be Met Asn Tyr Phe Be Read Glu Be Ile Ile

20 25 30

Leu Leu Leu Gly Leu Thr Wing Ser Leu Leu Leu Leu Pro Leu Met Leu

35 40 45

Pro Pro Leu Pro Pro Pro Phe Met Leu Leu Leu Val Pro Ile Phe

50 55 60

Ile Leu Val Val Leu Met Ile Leu Wing Phe Met Pro Ser Asn Val Arg 65 70 75 80

Asn Val Thr Cys Ser Tyr Leu 85

<210> 26 <211> 106 <212> PRT

<213> Arabidopsis thaliana <400> 26

Met Asp Val Gly Arg Asn Asn Arg Lys Asn Met Be Phe Arg Be Ser 15 10 15

Pro Glu Lys Be Lys Gln Glu Read Le Arg Arg Be Phe Be Wing Gln Lys 20 25 30

Arg Met Met Ile Pro Wing Asn Tyr Phe Ser Leu

35 40

Leu Val Gly Leu Thr Allah Be Leu Leu Ile Leu

50 55

Pro Leu Pro Pro Pro Phe Met Leu Leu 65 70 75

Met Val Leu Leu Val Val Leu Wing Phe Met Pro

85 90

Wing Asn Thr Asp Val Thr Cys Asn Phe Met 100 105

<210> 27

<211> 537

<212> DNA

<213> Oryza sativa

<400> 27

atgtacttgt tgagcccaag aaatggcgac ctgatcagcg acgacgagcc gcccaatctc agcagcagca gcagcggcag cgacatggag agtacggcgc cgccgccgcc gccgccgtcg agcaatatca gggaggcggc ggctagcggc tcggtggagt cgctgctcct gctggtgtgc gtgctgccgc cgctgccccc gccgccgtcg gtgctgctgc tggcgctggc gttcatgccg ggcggcggcg gcggcggccg caatggggcg <210> 28 <211> 538 <212> DNA <213> Zea mays <220>

<221> misc_feature <222> (177) .. (208) <223> η is a, c, g, <220>

<221> misc_feature <222> (493) .. (493) <223> η is a, c, g, <400> 28

ttcacattac actcatgact cgtcttagga ctggccaaga cctatttatc tatttacagg ggcatgaagg ccagtacaac cagcaagacg nnnnnnnnnn nnnnnnnnnn nnnnnnnncc caggcgagca cgaggaatgc cccgatgctg aggaaatccg cctcctgctg acgctgctgc attgccgccc ctccttccat cgcgctagat gaggtggaga ccagtcgcag tgagtggttg agaaccgcct tgngttttct gaacgttttg <210> 29 <211> 578 <212> DNA

<213> Vitis vinifera <400> 29

tttttttttt tttttttaat caagaaataa aaataacaaa aattgtatga tgagaagagg tgtataagtc aaatctctga cattagaagg aatgccaatg gggagcagaa gcagcatgaa gggaaggatc agcaatgaag ccgtgagaca atagcttgct gggatcagtc tcctgctgtt catcttcttt ccttcggcca taggaggagt agagttcctc cttcctctcg cttccatgat caaaggtcct tcagacagtc ttgaatctgc tcctttaccg gtcaccaagt tccaaccggt <210> 30 <211> 704

Glu Ser Leu Phe Leu 45

Pro Leu Val Leu Pro 60

Val Pro Ile Gly Ile 80

Ser Ser Ser His Asn 95

aacaggagga cctgcgccac gtaaagcctg aatccggcgg gcgtgtgggg cgctggtgat tggtgccggt cgtcgtcgtc atgctcccta

aatccaggag tgcagccagc cggcggcggg cggcggcggc caagtacttc cctcccgctc ggcgatgctg gtccgccggc cttgtag

or t

or t

cgaatcctgc taagaggagg agcaggcaga agcggcagta aagtaggtgg agatgccgct cggcttgcca ccaaagtaag taatcagatc

agctgcaaaa ccatgctgcg ccggcagcca tcagcagcga acgcgatgac tcacggcatt tgtgttcttt caagaagtga tgagctcggg

cacagaaaat cacatctgtc cagcagnnnn gacggtgagg cttcttgtcg cctcctttgt ggtgtaggcg aaggcggtgt tggtcgaa

aataactgat tgcacttttg catgaaagcc aggagggggt gatgagcaaa ctctcgtgag agggtttttc gccactcaaa ttcaggctgc tcaaagta

tttcatctga aacaccacca aagatcataa ggcaatggtg agcaatgact aggggagttc ctctcaactt tatcgatctt tcaatactca

atatcaagac tttacacata gcactgctag gtagtatcag ccaagctgaa tattatattc gcagaggagc gcagattgat tttaaatttc

60 120 180 240 300 360 420 480 537

60 120 180 240 300 360 420 480 538

60 120 180 240 300 360 420 480 540 578 <212> DNA

<213> Citrus reticulata <220>

<221> misc_feature <222> (619) .. (619) <223> η is a, c, g, or t <400> 30

agggtttctt caaagatagg tagccatttg cacatttgaa tctgcttgtt ggatattgtc 60

aaggaggctg ttggaattag gccacatttc agaatctggt ttcatcctgg atcgctgggc 120 atttgaaggc attttgtgat catcgctgtt taaaatttgg ccgcatatta gaatctgggt 180 tctcatcggt tttccgtaca tttaccttga ccacattttg atatctgggt tgagctgcat 240 tttagccttc gtatttaaaa ggacttgatc taatctgggg tcttggtgag ccggggcaac 300 tgatcatagt aaatgaattc tgataattct gagtcgagac agagactatc aaagggcatt 360 ataaacttgc aagatcgata tccgaccagc attatggatc gtggtgtaag aaaaattgca 420 actcctccgg tcgagaagag gaaagttgag tatcaccgaa gttactcgca aggggcatcc 480 agaaaactgt tttcggcaag ctatttcacc ctggaatcat tgcttttgct cgtatgtctg 540 acggcctcat tgctgatcct gccattggtg cttccgccct tgccgccccc gccattcctg 600 ctgcttctgg ttcctatang tattctagcc gtgcttttgg tcttggcatt catgccttct 660 aatgtaagag atataacttc cacgtacgtg taaatggtgt TGCT 704

<210> 31 <211> 382 <212> DNA

<213> Lycorpersicon esculentum <400> 31

gaaaaaaatg tatttaatca ttatgtaaaa aacaagtgaa tctactttga tattttcttc 60

taaattaaac cacacaatta aagatatgag caagtcacat tcctaacatt agaaggcata 120 aaagctaaga tcataagaac aacaagaatg aaaattggga ctaacaacaa cataaaaggt 180 ggtggtggca atggtggaag catcaatggc aaaagtaaca aagatgctgt aagaccaagt 240 aacaaaataa ttgactctaa gctaaaataa ttcattgaca acattttctt gccatgtcct 300 tgtgtaaatg atctcttatg ctccatctta ttgccttcca taatgttgtt gaaatattgt 360 aa 382 tgatgttcct ccaaattaat

<210> 32 <211> 624 <212> DNA

<213> Lycorpersicon esculentum <400> 32

tttgttaaag attggcacat tttcaagttc agtattcatt cgatttttga tatctacata 60

aaaaaaaagt gtcctggtac tactcaatat tcctcagaac gacttcatat tcaggtctcg 120 aattcaaaac ctcacatcaa gattcttagg aaatttcaag attggttgaa aaactcatat 180 ccttctctaa gtttcaagat tggttccaaa ttaaaactcg agacttctga gtaagagcgt 240 acgactagta atgaacatgg acatggaatc atcagaggca aaattgagat catcaaaagg 300 gtttattaat ttggaggaac atcaacaata tttcaacaac attatggaag gcaataagat 360 ggagcataag agatcattta cacaaggaca tggcaagaaa atgttgtcaa tgaattattt 420 tagcttagag tcaattattt tgttacttgg tcttacagca tctttgttac ttttgccatt 480 gatgcttcca ccattgccac caccaccttt tatgttgttg ttagtcccaa ttttcattct 540 tgttgttctt atgatcttag cttttatgcc ttctaatgtt aggaatgtga cttgctcata 600 tctttaattg tgtggtttaa ttta 624

<210> 33 <211> 1130 <212> DNA <213> Oryza sativa <400> 33

catgcggcta atgtagatgc tcactgcgct agtagtaagg tactccagta cattatggaa 60

tatacaaagc tgtaatactc gtatcagcaa gagagaggca cacaagttgt agcagtagca 120 caggattaga aaaacgggac gacaaatagt aatggaaaaa caaaaaaaaa caaggaaaca 180 catggcaata taaatggaga aatcacaaga ggaacagaat ccgggcaata cgctgcgaaa 240 gtactcgtac gtaaaaaaaa gaggcgcatt catgtgtgga cagcgtgcag cagaagcagg 300 gatttgaaac cactcaaatc caccactgca aaccttcaaa cgaggccatg gtttgaagca 360 tagaaagcac aggtaagaag cacaacgccc tcgctctcca ccctcccacc caatcgcgac 420 gcacctcgcg gatcggtgac gtggcctcgc cccccaaaaa tatcccgcgg cgtgaagctg 480 acaccccggg cccacccacc tgtccgggggggggggggggggggggggggg

Pro Pro Leu Pro Pro 30

Ile Met Wing Leu Wing 45

Lys Asn Val Val Val 60

Lys arg

tgtacgtggg cgggttaccc tgggggtgtg ggtggatgac gggtgggccc ggaggaggtc 720 cggccccgcg cgtcatcgcg gggcggggtg tagcgggtgc gaaaaggagg cgatcggtac 780 gaaaattcaa attaggaggt ggggggcggg gcccttggag aataagcgga atcgcagata 840 tgcccctgac ttggcttggc tcctcttctt cttatccctt gtcctcgcaa ccccgcttcc 900 ttctctcctc tcctcttctc ttctcttctc tggtggtgtg ggtgtgtccc tgtctcccct 960 ctccttcctc ctctcctttc ccctcctctc ttcccccctc tcacaagaga gagagcgcca 1020 gactctcccc aggtgaggtg agaccagtct ttttgctcga ttcgacgcgc ctttcacgcc 1080 gcctcgcgcg gatctgaccg cttccctcgc ccttctcgca ggattcagcc 1130

<210> 34 <211> 77 <212> PRT

<213> Medicago sativa <400> 34

Met Val Arg Cys Phe Ser Leu Gly Ser Val Leu 15 10

Wing Wing Ser Met Val Val Leu Pro Leu Met Leu

20 25

Pro Pro Leu Wing Leu Leu Phe Phe Pro Val Gly

35 40

Val Val Leu Wing Phe Ser Pro Glu Asn Val

50 55

Tyr Ser Ser Ser Ser Gly Ile Wing Asn Ser 65 70 75

<210> 35 <211> 78 <212> PRT

<213> Medicago sativa <400> 35

Met Ile Met Val Wing Be Lys Glu Lys Thr Asn 15 10

Phe Arg Tyr Ser Leu Ile Leu Ser Leu Leu

20 25

Val Leu Pro Leu Val Met Pro Leu Pro

35 40

Leu Leu Val Pro Val Phe Ile Met Leu Leu Leu

50 55

Ser Pro Lys Lys Val Pro Asn Lys Ala Ser 65 70 75

<210> 36 <211> 613 <212> DNA

<213> Hordeum vulgare <400> 36

cttccgagaa agatgcttca tattgcactc atcttatgcc aacacacaat cagaaacaaa 60

aaccacgcag caagcctatt tacaagtaag aggtggccat gctccgcaca tcagtcggca 120 tgaaggccag caccatcaac aggatgagca agcagaccgg caagagcagc agtagcgatg 180 gcggtggggg caacggaggc agcaccaatg gcagtatgag caatgaaacg gtgaggcagg 240 cgagcacgaa gaacgcttcg acgccgaagt agttcggtat gacaaccttc ttgtcagcag 300 catttgccct ctgctgctgc atttgcctct ttgcagtatt tggtctggct aacacagggc 360 tgctcctctg cctactaccg cctctgtcca ttgcaccgaa ctggctatcc atctattctt 420 tggtgagtgc agatcagcgg ctatccagga actgagatga ataagaactt gtggaatctg 480 aaggttttta acagatctga agtctttgtg agtccgtgac tgaactgcag atcatctgct 540 gtggaagaac tgcaccgcaa gtggcaatac cttcgatcct gaaacttgca ttttggtgat 600 gcccgtacca cgc 613

<210> 37 <211> 617 <212> DNA

<213> Saccharum officinarum <220>

<221> misc_feature <222> (451) .. (451) <223> η is a, c, g, or t <400> 37

Ser Gly Gly Cys Met 15

Wing Leu Ser Ile Leu 30

Pro Pro Leu Leu Leu 45

Phe Phe Ile Ala Phe 60

Phe Val Ser ggagaacgtg cttgttcagg tggttcaaca gtgcggacca gagaacaatg cgaggttcag 60

gattaaaggt attctggctt cagaggcagt tcttccaaag caagtgacag gcgattccat 120

caccggagct aagatcttat tacaacattc agaaacacag gagtcctccc accgcctttc 180

acttcttgct tacttctgca accactcgcc gtgactgatc tccacgcaca ccaaagaaca 240

caacacgtgg caagccgatc tagcgcgatg gaaggagggg ggcaaataca gaggaggaat 300

aatgccgtga agcggcacct gcagcagcgg cagcaggagg cggatttcct cgacaagaag 360

gtcatcgcgt ccacctattt cagcatcgag gcgttcctcg tgctcgcctg cctcaccgtc 420

tcgctgctga tactgccgct tgtgctgccg ncgctgccgg cgccggcgtc gctgctgctg 480

tggctgccgg tctggctgct cgaactgctg attgtgcttg ccttcatgcc gacagatgtg 540

cgcagcatgg cctcctctta cttgtaaaaa aatagataaa taggccacct ttggcaattt 600

tctggggttt ggaggtg 617 <210> 38 <211> 638 <212> DNA

<213> Saccharum officinarum <400> 38

gattttttcc aagccagtga ccggcgattc atcaaccgga gctgagatct tatacaacat 60

tcagaaacac aggagtcctc gcaccgcttt ccactcttgg ctaattttgc aaccactcgc 120

cgtgactgat ctccacgcac accaaagaac acaacacgtg gcaacccgat ctagcgcgat 180

ggaaggaggg gggcaaatac agaggaggaa taatgccgtg aagcggcacc tgcagcagcg 24 0

gcagcaggag gcggatttcc tcgacaagaa ggtcatcgcg tccacctatt tcagcatcga 300

ggcgttcctc gtgctcgcct gcctcaccgt ctcgctgctg atactgccgc tggtgctgcc 360

gccgctgccg ccgccgccgt cgctgctgct gtggctgccg gtctgcctgc tcatcctgct 420

gattgtgctg gccttcatgc cgacagatgt gcgcagcatg gcctcctctt acttgtaata 480

aatagataaa taggccacct tggtcaatat tctgtgattt ggaggtgatt cgtcctgaga 540

tgagtctcga ttgatgtcag ctactcccaa ggggaaatgc atgtaacact tggtggccga 600

cggtggcaag ataatcatgc taccatgtca gttaaacc 638 <210> 39 <211> 778 <212> DNA

<213> Sorghum bicolor <400> 39

gctgttggtg ttgttcttga gatctctttc ttgatcttgt gtgggattaa agagggattc 60

ttgccttcct acgggagaaa gagaaaaggg gaagaacgtg cttgttccgg tggttcaaca 120

gtgcggagac ccggagaaca atgcgaggtt caggattaaa ggtgttctgg cttcaggtgc 180

agttcttcca aagcaggtga caggcgattc gatcaccgga gctcagatct gacgaaaaca 240

aaacacagtc ctcctcccac cgcctttcag ttcttgctta cttctgcaac cactcactgc 300

gaccgtacac caaagaacac tgcacatggc aagccgatct agcgcgctgg aaggaggggg 360

ggcagcaata cagcggagga ataatgccgt gaagcggcac ctgcagcagc ggcagcagga 420

ggcggatttc cacgacaaga aggtcatcgc gtccacctac ttcagcatcg gcgcgttcct 480

ggtgctcgcc tgcctcacct tctcgctgct catcctgccg ctggtgctgc cgccgctgcc 540

gccgccgccg tcgctgctgc tgtggctgcc ggtctgcctg ctcgtcctgc tggttgtgct 600

ggccttcatg ccgacagatg tgcgcagcgt ggcggcctct tacttgtaaa tagccagata 660

aataggccac cacctttggc cagttttctg tgttttcggg ggtgattcgt cctaagatga 720

gtcatgatcg agtgtaatgt gaagcatctt ttccaggggt agtagatttc aatgaagt 778 <210> 40 <211> 732 <212> DNA

<213> Arabidopsis thaliana <400> 40

ttgtcttcct catttcccta ctagtacttg tttcacacag tttcttgatc caaccaaaac 60

caatacacaa agcttctcaa actccttcac ctcaaagctt cttcctttac atctgaatcg 120

ttgagttaac tcggatttgt tctgcatcct · ctgtttctga atcgtgggcc atccttattt 180

tgtctcgaat tcttcaccaa ttgcttcgat caagctgcat tggttaacca gttgccctaa 240

agatcagatc tttgagcaaa attttgtcac tgatcttcta aatccaaacc agacacagca 300

aaacaacctc tgtagatgat tcgagaaatc tcaaacttac aaaaagatat tataaacatt 360

caagacagtt attcgaacaa ccgagtcatg gacgtcggaa gaaacaaccg gaaaaacatg 420

agctttcgaa gttcgccgga gaaaagcaag caagagttac ggcggagttt ctcggcgcag 480

aaaaggatga tgatcccggc gaattatttc agtttagagt ctctgttcct attggttggt 540

ctaacggcat ctctgttaat acttccgtta gttttgccgc cgttacctcc gcctccgttt 600

atgctgctat tggttcccat tgggattatg gttttactcg tcgttcttgc cttcatgcct 660

tcttctcatt ctaatgctaa tacagatgta acttgcaatt tcatgtaaat ctgaaattta 720

ttatatgatg at 732 <210> 41 <211> 891 <212> DNA

<213> Arabidopsis thaliana <400> 41

cccactttat ttcttcttct ctggttttct taccaaaaga gtatttaagc tttaacaccc tgtttttggt ttccaacgtt ctgaaggtgt gtttggctct aacggtttaa agctttcttg ctctctaccg gcaaaaaaaa aagattagtc cttttaggtc gttctaaacc ttagattttg tctgcatttc gggataatca aaccaaacta catttacaga agaagaagaa gaagaaaagt ggataaacaa actcaagttt cttcttcata catcgatctg aaaagagaaa aagccttctt taaatgattc gtgagttctc taaacattca agaacattat tctctcaaca acaacatgga ggaaaaacac gagttttcgt ggttcagctc cagctccgat ttcggacatt gtcgtcgcag aacagtccaa ggaggctaat tagaatcaat ggttgtgctt gttggtctca cagcatctct ttccaccatt gcctcctcct ccttttatgc tgcttttgat tgcttatggt tcttgctttc atgccttctt ctaattccaa cttttatgta ataaacgttt ctttaattga agaaagaaat <210> 42 <211> 732 <212> DNA

<213> Arabidopsis thaliana <400> 42

ttgtcttcct catttcccta ctagtacttg tttcacacag caatacacaa agcttctcaa actccttcac ctcaaagctt ttgagttaac tcggatttgt tctgcatcct ctgtttctga tgtctcgaat tcttcaccaa ttgcttcgat caagctgcat agatcagatc tttgagcaaa attttgtcac tgatcttcta aaacaacctc tgtagatgat tcgagaaatc tcaaacttac caagacagtt attcgaacaa ccgagtcatg gacgtcggaa agctttcgaa gttcgccgga gaaaagcaag caagagttac aaaaggatga tgatcccggc gaattatttc agtttagagt ctaacggcat ctctgttaat acttccgtta gttttgccgc atgctgctat tggttcccat tgggattatg gttttactcg tcttctcatt ctaatgctaa tacagatgta acttgcaatt ttatatgatg at <210> 43 <211> 519 <212> DNA <213> Zea mays <400> 43

atgcgaggtt cgggatttaa gatgttcggc tttaggggca gggcgattcg accaccggag ctcagatctg attacaaaac tctcacaccg cctttcactt cttgcttact ttggcaacca cctccaccta caccaagaac acatggcaag ccgatctacg atacaaagga ggaatgccgt gaagcggcat ctgcagcagc ctcgacaaga aggtcatcgc gtccacctac ttcagcatcg tgcctcaccg tctcgctgct gatactgccg ctggtgctgc tcgctgctgt tgtggctgcc ggtctgcctg ctcgtcttgc ccgacagatg tgcgcagcat ggcctcctct tacctgtaa <210> 44 <211> 98 <212> PRT <213 > Zea mays <400> 44

Met Glu Gly Gly Wing Wing Ile Gln Arg Arg Asn 1 5 10

Leu Gln Gln Arg Gln Gln Glu Wing Asp Phe Leu

20 25

Ala Ser Thr Tyr Phe Ser Ile Gly Ala Phe Leu 35 40

aactttcttc caatcttcat aaacaccaat tggaaacgcc tttcatcgtc tcgttacttt attttccaga cagtctacaa cgtgagagga tatggggaag atcagcgagt cttgatctta tcctattggg acatgtttct ccttaaacaa

gtcttcctct cttcttctcg tgaatctttt aagatcactc agggttcttc ttatgcgttt tcaaacttcg aacgacatca gatcataacc caagaattgt tacttcagtt ccgttgattc attatggttt tcttcttcca

tttcttgatc cttcctttac atcgtgggcc tggttaacca aatccaaacc aaaaagatat gaaacaaccg ggcggagttt ctctgttcct cgttacctcc tcgttcttgc tcatgtaaat

caaccaaaac atctgaatcg atccttattt gttgccctaa agacacagca tataaacatt gaaaaacatg ctcggcgcag attggttggt gcctccgttt cttcatgcct ctgaaattta

gttcttctga gttcagaaaa ctcactgcga cgatggaagg gtcagcagga gggcgttcct ctcccctgcc tggttgtact

agcaggggac cacaaggcgt ctggtctcca aggggcggca ggcggatttc cgtgctcgcc gccgccgccg ggccttcatg

Wing Val Lys Arg His 15

Asp Lys Lys Val Ile 30

Val Leu Wing Cys Leu 45

60 120 180 240 300 360 420 480 540 600 660 720 780 840 891

60 120 180 240 300 360 420 480 540 600 660 720 732

60 120 180 240 300 360 420 480 519 Thr Val Ser Leu Leu Ile Leu Pro Leu Val Leu Pro Pro Leu Pro Pro

50 55 60

Pro Pro Ser Leu Leu Leu Trp Leu Pro Val Cys Leu Leu Val Leu Leu 65 70 75 80

Val Val Leu Wing Phe Met Pro Thr Asp Val Arg Be Met Wing Be Ser 85 90 95

Tyr leu

<210> 45 <211> 2745 <212> DNA

<213> Arabidopsis thaliana <400> 45

atgagcaatg cttttttata atgccaactt tgtacaaaaa agcaggctta aacaatgaaa 60

tctcgagcga ggcagtgtct gctggtttgt ttgctctgtc tttccttaac gaatctctcc 120

tatggagaaa ataagttcag agagcgtaaa gccaccgatg acgagctggg ctaccccgat 180

attgatgaag atgctttatt gaatactcag tgcccgaaaa aattggagct gcgatggcaa 240

actgaagtca cttctagcgt ttatgctaca cccttgattg ctgatattaa cagtgatgga 300

aagcttgaca ttgttgttcc atcttttgtt cattacctcg aagttcttga aggagctgat 360

ggagacaaga tgccaggttg gcctgctttt caccagtcaa atgtgcactc gagtcctctt 420

ctatttgata tcgacaaaga tggtgttaga gaaattgctc tggctaccta caatgccgaa 480

gtgctctttt tcagggtatc aggctttttg atgtcagata agctagaagt gccacgtaga 540

aaagtgcaca agaactggca tgtgggactt aatcctgatc ctgttgaccg ttcacatcct 600

gatgttcatg atgatgtgct tgaggaggaa gctatggcaa tgaagtcatc gaccactcaa 660

acgaatgcaa ctaccacaac accaaatgtt acagtctcga tgaccaaaga agttcatggc 720

gctaattcat atgtgtcaac tcaagaggat caaaagagac cagagaataa tcaaacagaa 780

gctattgtaa agcctactcc agagctacat aattcctcca tggatgctgg agcaaataat 840

ttggcagcaa atgctactac agctggctca agagaaaacc tcaatagaaa tgtaaccacc 900

aatgaggtgg atcaaagcaa aattagtgga gataagaatg aaactgttat taaattaaat 960

actagtacgg gtaattcctc agaaactctg gggacatctg gaaacagtag tacggcagag 1020

acagtaacca aaagtgggag gcgacttctg gaagaggatg gttcgaaaga atctgtggac 1080

agccattcgg acagtaaaga caacagtgag ggtgtccgca tggcgacagt agaaaatgat 1140

ggaggcttag aagctgacgc agattcatcg tttgagttgt tgcgtgagaa tgatgagtta 1200

gctgatgaat acagttatga ttatgacgat tatgttgatg agaaaatgtg gggtgatgag 1260

gaatgggttg aggggcagca cgagaactca gaagattatg tgaatattga cgcccatata 1320

ctatgcactc ctgtaattgc tgacatagac aaagatggag tacaggagat gattgttgct 1380

gtttcctatt tcttcgaccc cgagtactat gataatccag aacatctgaa agagcttggt 1440

ggtatcgaca ttaaaaatta tattgctagt tcaattgtgg ttttcaatct tgatactaaa 1500

caagtcaagt ggatcaaaga gctagatttg agtacggata aagcaaactt ccgtgcttat 1560

atttattctt caccaacggt tgttgatttg gatggcgatg gttatttgga tatccttgtc 1620

ggaacttcct ttggcttatt ctacgccatg gatcatcgtg gaaacatcag agaaaaattc 1680

ccactggaaa tggctgaaat tcaaggagca gtggttgcgg ccgacataaa tgatgatgga 1740

aagattgaac ttgtaactac tgattcacac ggaaatatag cagcatggac cacccaagga 1800

gtggaaattt gggaagcaca tcttaagagc cttgttcccc agggtccttc tataggcgat 1860

gttgatggtg acggacacac ggaggttgtg gttcctacat catcaggaaa catatacgtt 1920

cttagtggca aggatggttc tattgtccgt ccttacccat acaggactca tggaagagtg 1980

atgaaccaac ttcttcttgt tgatctgaac aagcgaggtg agaaaaagaa gggtctcacc 2040

atcgttacta catcctttga cggttacctg tatctcatag atggacccac ctcgtgcact 2100

gacgttgttg acattggcga aacttcatac agcatggtct tggctgataa tgttgacggt 2160

ggagatgatc tcgatcttat tgtctcaact atgaatggaa acgtcttttg cttctcaacg 2220

ccttcttctc accatcccct taaggcttgg agatctagtg atcaaggcag gaacaataag 2280

gccaatcgtt atgatcgtga aggcgttttt gtcacgcatt cgaccagagg tttccgtgat 2340

gaggaaggca aaaacttctg ggctgagatc gagatcgttg ataaatatag atatccatct 2400

ggttcacaag caccctacaa cgttactacg acgctgttgg ttccaggcaa ttaccaggga 2460

gagaggagga taacgcagag ccagatctat gaccgccctg gaaaataccg gataaaacta 2520

ccaactgtgg gagtgagaac aacaggaact gtaatggtgg agatggcaga taagaatgga 2580

ctccatttct cagacgaatt ctcactaact ttccatatgt attactacaa gcttctgaaa 2640

tggcttcttg ttctcccgat gctcgggatg ttcggtctgc tcgtgatact acggcctcaa 2700

gaagccgtgc ctctcccgtc tttttcccgc aacacagatt tatga 2745 <210> 46 <211> 896 <212> PRT

<213> Arabidopsis thaliana <400> 46

Met Lys Ser Arg Arg Wing Gln Cys Leu Leu Val Cys Leu Leu Cys Leu 1 5 10 15 Ser Leu Thr Asn Leu Ser Tyr Gly Glu Asn Lys Phe Arg Glu Arg Lys 20 25 30 Wing As Thr Asp Glu Leu Gly Tyr Pro Asp Ile Asp Glu Asp Wing Leu 35 40 45 Read Asn Thr Gln Cys Pro Lys Lys Leu Glu Leu Arg Trp Gln Thr Glu 50 55 60 Val Thr Be Ser Val Tyr Wing Pro Pro Ile Wing Asp Ile Asn Ser 65 70 75 80 Asp Gly Lys Leu Asp Ile Val Val Pro Ser Phe Val His Tyr Leu Glu 85 90 95 Val Leu Glu Gly Ala Asp Gly Asp Lys Met Pro Gly Trp Pro Ala Phe 100 105 110 His Gln Ser Asn Val His Ser Ser Pro Leu Phe Asp Ile Asp Lys 115 120 125 Asp Gly Val Arg Glu Ile Wing Leu Thr Wing Tyr Asn Wing Glu Val Leu 130 135 140 Phe Phe Arg Val Ser Gly Phe Leu Met Ser Asp Lys Leu Glu Val Pro 145 150 155 160 Arg Lys Val His Lys Asn Trp His Val Gly Read Asn Pro Asp Pro 165 170 175 Val Asp Arg Be His Pro Asp Val is Asp Asp Val Leu Glu Glu Glu 180 185 190 Wing Met Wing Met Lys Being Ser Thr Thr Gln Thr Asn Wing Thr Thr Thr 195 200 205 Thr Pro Asn Val Thr Val Being Met Thr Lys Glu Val His Gly Wing Asn 210 215 220 Ser Tyr Val Ser Thr Gln Glu Asp Gln Lys Arg Pro Glu Asn Asn Gln 225 230 235 240 Thr Glu Wing Ile Val Lys Pro Thr Glu Leu His Asn Ser Be Met 245 250 255 Asp Wing Gly Wing Asn Asn Leu Wing Wing Asn Wing Thr Thr Wing Gly Ser 260 265 270 Arg Glu Asn Leu Asn Arg Asn Val Thr Thr Asn Glu Val Asp Gln Ser 275 280 285 Lys Ile Ser Gly Asp Lys Asn Glu Thr Val Ile Lys Leu Asn Thr Ser 290 295 300 Thr Gly Asn Ser Ser Glu Thr Leu Gly Thr Be Gly Asn Ser Be Thr 305 310 315 320 Wing Glu Thr Val Lys Be Gly Arg Arg Leu Read Leu Glu Glu Asp Gly 325 330 335 Be Lys Glu Be Val Asp Be His Asp Be Lys Asp Asn Be Glu 340 345 350 Gly Val Arg Met Wing Thr Val Glu Asn Asp Gly Gl and Leu Glu Wing Asp 355 360 365 Wing Asp Be Ser Phe Glu Leu Read Le Arg Glu Asn Asp Glu Leu Wing Asp 370 375 380 Glu Tyr Ser Tyr Asp Tyr Asp Tyr Val Asp Glu Trp Val Glu Gly Gln His Glu Asn Ser Glu Asp Tyr Val 405 410 415 Asn Ile Asp Wing His Ile Leu Cys Thr Pro Val Ile Wing Asp Ile Asp 420 425 430 Lys Asp Gly Val Gln Glu Met Wing Asp 435 440 445 Pro Glu Tyr Tyr Asp Pro Glu His Leu Lys Glu Leu Gly Gly Ile 450 455 460 Asp Ile Lys Asn Tyr Ile Wing Ser Ser Ile Val Val Phe Lys Glu Leu Asp Leu Ser Thr Thr Asp Lys 485 490 495 Asn Phe Arg Wing Tyr Ile Tyr Ser Ser Thr Thr Val Val Asp Leu

500 505 510

Asp Gly Asp Gly Tyr Leu Asp Ile Leu Val Gly Thr Be Phe Gly Leu

515 520 525

Phe Tyr Ala Met Asp His Arg Gly Asn Ile Arg Glu Lys Phe Pro Leu

530 535 540

Glu Met Wing Glu Ile Gln Gly Wing Val Val Wing Wing Asp Ile Asn Asp 545 550 555 560

Asp Gly Lys Ile Glu Read Val Thr Thr Asp Be His Gly Asn Ile Wing

565 570 575

Trp Wing Thr Thr Gln Gly Val Glu Ile Trp Glu Wing His Leu Lys Ser

580 585 590

Read Val Pro Gln Gly Pro Ser Ile Gly Asp Val Asp Gly Asp Gly His

595 600 605

Thr Glu Val Val Val Pro Thr Be Ser Gly Asn Ile Tyr Val Leu Ser

610 615 620

Gly Lys Asp Gly Ser Ile Val Arg Tyr Pro Tyr Tyr Arg Thr His Gly 625 630 635 640

Arg Val Met Asn Gln Leu Leu Leu Val Asp Leu Asn Lys Arg Gly Glu

645 650 655

Lys Lys Lys Gly Leu Thr Ile Val Thr Thr Be Phe Asp Gly Tyr Leu

660 665 670

Tyr Leu Ile Gly Asp Pro Thr Be Cys Asp Val Val Asp Ile Gly

675 680 685

Glu Thr Be Tyr Be Met Val Leu Wing Asp Asn Val Asp Gly Gly Asp

690 695 700

Asp Leu Asp Leu Ile Val Be Thr Met Asn Gly Asn Val Phe Cys Phe 705 710 715 720

Be Thr Pro Be Be His His Pro Read Lys Wing Trp Arg Be Be Asp

725 730 735

Gln Gly Arg Asn Asn Lys Wing Asn Arg Tyr Asp Arg Glu Gly Val Phe

740 745 750

Val Thr His Ser Thr Arg Gly Phe Arg Asp Glu Glu Gly Lys Asn Phe

755 760 765

Trp Wing Glu Ile Glu Ile Val Asp

770 775 780

Gln Wing Pro Tyr Asn Val Thr Thr Thr Read Leu Val Pro Gly Asn Tyr 785 790 795 800

Gln Gly Glu Arg Arg Ile Thr Gln Be Gln Ile Tyr Asp Arg Pro Gly

805 810 815

Lys Tyr Arg Ile Lys Read Pro Thr Val Gly

820 825 830

Val Met Val Glu Met Wing Asp Lys Asn Gly Read His Phe Ser Asp Glu

835 840 845

Phe Ser Leu Thr Phe His Met Tyr Tyr Tyr Lys Leu Leu Lys Trp Leu

850 855 860

Leu Val Leu Pro Met Leu Gly Met Phe Gly Leu Leu Val Ile Leu Arg 865 870 875 880

Pro Gln Glu Wing Val Pro Leu Pro Be Phe Ser Arg Asn Thr Asp Leu 885 890 895

<210> 47

<211> 53

<212> DNA

<213> Artificial sequence

<220>

<223> initiator: prm06643

<400> 47

ggggacaagt ttgtacaaaa aagcaggctt aaacaatgaa atctcgagcg agg <210> 48

<211> 52

<212> DNA

<213> Artificial sequence

<220> <223> initiator: prm06644 <400> 48

ggggaccact ttgtacaaga aagctgggtc ctgtttacag atggtaccta gt 52 <210> 49 <211> 4938 <212> DNA

<213> Artificial sequence <220>

<223> expression cassette <400> 49

aatccgaaaa gtttctgcac cgttttcacc ccctaactaa caatataggg aacgtgtgct 60

aaatataaaa tgagacctta tatatgtagc gctgataact agaactatgc aagaaaaact 120

catccaccta ctttagtggc aatcgggcta aataaaaaag agtcgctaca ctagtttcgt 180

tttccttagt aattaagtgg gaaaatgaaa tcattattgc ttagaatata cgttcacatc 240

tctgtcatga agttaaatta ttcgaggtag ccataattgt catcaaactc ttcttgaata 300

aaaaaatctt tctagctgaa ctcaatgggt aaagagagag atttttttta aaaaaataga 360

atgaagatat tctgaacgta ttggcaaaga tttaaacata taattatata attttatagt 420

ttgtgcattc gtcatatcgc acatcattaa ggacatgtct tactccatcc caatttttat 480

ttagtaatta aagacaattg acttattttt attatttatc ttttttcgat tagatgcaag 540

gtacttacgc acacactttg tgctcatgtg catgtgtgag tgcacctcct caatacacgt 600

tcaactagca acacatctct aatatcactc gcctatttaa tacatttagg tagcaatatc 660

tgaattcaag cactccacca tcaccagacc acttttaata atatctaaaa tacaaaaaat 720

aattttacag aatagcatga aaagtatgaa acgaactatt taggtttttc acatacaaaa 780

aaaaaaagaa ttttgctcgt gcgcgagcgc caatctccca tattgggcac acaggcaaca 840

acagagtggc tgcccacaga acaacccaca aaaaacgatg atctaacgga ggacagcaag 900

tccgcaacaa ccttttaaca gcaggctttg cggccaggag agaggaggag aggcaaagaa 960

aaccaagcat cctcctcctc ccatctataa attcctcccc ccttttcccc tctctatata 1020

ggaggcatcc aagccaagaa gagggagagc accaaggaca cgcgactagc agaagccgag 1080

cgaccgcctt cttcgatcca tatcttccgg tcgagttctt ggtcgatctc ttccctcctc 1140

cacctcctcc tcacagggta tgtgcccttc ggttgttctt ggatttattg ttctaggttg 1200

tgtagtacgg gcgttgatgt taggaaaggg gatctgtatc tgtgatgatt cctgttcttg 1260

gatttgggat agaggggttc ttgatgttgc atgttatcgg ttcggtttga ttagtagtat 1320

ggttttcaat cgtctggaga gctctatgga aatgaaatgg tttagggtac ggaatcttgc 1380

gattttgtga gtaccttttg tttgaggtaa aatcagagca ccggtgattt tgcttggtgt 1440

aataaaagta cggttgtttg gtcctcgatt ctggtagtga tgcttctcga tttgacgaag 1500

ctatcctttg tttattccct attgaacaaa aataatccaa ctttgaagac ggtcccgttg 1560

atgagattga atgattgatt cttaagcctg tccaaaattt cgcagctggc ttgtttagat 1620

acagtagtcc ccatcacgaa attcatggaa acagttataa tcctcaggaa caggggattc 1680

cctgttcttc cgatttgctt tagtcccaga attttttttc ccaaatatct taaaaagtca 1740

ctttctggtt cagttcaatg aattgattgc tacaaataat gcttttatag cgttatccta 1800

gctgtagttc agttaatagg taatacccct atagtttagt caggagaaga acttatccga 1860

tttctgatct ccatttttaa ttatatgaaa tgaactgtag cataagcagt attcatttgg 1920

attatttttt ttattagctc tcaccccttc attattctga gctgaaagtc tggcatgaac 1980

tgtcctcaat tttgttttca aattcacatc gattatctat gcattatcct cttgtatcta 2040

cctgtagaag tttctttttg gttattcctt gactgcttga ttacagaaag aaatttatga 2100

agctgtaatc gggatagtta tactgcttgt tcttatgatt catttccttt gtgcagttct 2160

tggtgtagct tgccactttc accagcaaag ttcatttaaa tcaactaggg atatcacaag 2220

tttgtacaaa aaagcaggct taaacaatga aatctcgagc gaggcagtgt ctgctggttt 2280

gtttgctctg tctttcctta acgaatctct cctatggaga aaataagttc agagagcgta 2340

aagccaccga tgacgagctg ggctaccccg atattgatga agatgcttta ttgaatactc 2400

agtgcccgaa aaaattggag ctgcgatggc aaactgaagt cacttctagc gtttatgcta 2460

cacccttgat tgctgatatt aacagtgatg gaaagcttga cattgttgtt ccatcttttg 2520

ttcattacct cgaagttctt gaaggagctg atggagacaa gatgccaggt tggcctgctt 2580

ttcaccagtc aaatgtgcac tcgagtcctc ttctatttga tatcgacaaa gatggtgtta 2640

gagaaattgc tctggctacc tacaatgccg aagtgctctt tttcagggta tcaggctttt 2700

tgatgtcaga taagctagaa gtgccacgta gaaaagtgca caagaactgg catgtgggac 2760

ttaatcctga tcctgttgac cgttcacatc ctgatgttca tgatgatgtg cttgaggagg 2820

aagctatggc aatgaagtca tcgaccactc aaacgaatgc aactaccaca acaccaaatg 2880

ttacagtctc gatgaccaaa gaagttcatg gcgctaattc atatgtgtca actcaagagg 2940

atcaaaagag accagagaat aatcaaacag aagctattgt aaagcctact ccagagctac 3000

ataattcctc catggatgct ggagcaaata atttggcagc aaatgctact acagctggct 3060

caagagaaaa cctcaataga aatgtaacca ccaatgaggt ggatcaaagc aaaattagtg 3120

gagataagaa tgaaactgtt attaaattaa atactagtac gggtaattcc tcagaaactc 3180 tggggacatc tggaagagga agggtgtccg cgtttgagtt attatgttga cagaagatta acaaagatgg atgataatcc gttcaattgt tgagtacgga tggatggcga tggatcatcg cagtggttgc acggaaatat gccttgttcc tggttcctac gtccttaccc acaagcgagg tgtatctcat acagcatggt ctatgaatgg ggagatctag ttgtcacgca tcgagatcgt cgacgctgtt atgaccgccc ctgtaatggt ctttccatat tgttcggtct gcaacacaga <210> 50 <211> 10 <212> PRT < 213> Artificial sequence <220>

<223> Subject retained 1 <220>

<221> VARIANT

<222> (9) .. (9)

<223> / replace = "Glu"

<400> 50

Phe Asp Gly Tyr Leu Tyr Leu Ile Asp Gly 15 10

<210> 51 <211> 5 <212> PRT

<213> Artificial sequence <220>

<223> Subject retained 2 <220>

<221> UNSAFE <222> (3) .. (4)

<223> Xaa can be any naturally occurring amino acid <220>

<221> VARIANT <222> (5) .. (5) <223> / replace = "Glu" <400> 51

Asp Gly Xaa Xaa Asp 1 5

<210> 52 <211> 9 <212> PRT

tggaaacagt tggttcgaaa catggcgaca gttgcgtgag tgagaaaatg tgtgaatatt agtacaggag agaacatctg ggttttcaat taaagcaaac tggttatttg tggaaacatc ggccgacata agcagcatgg ccagggtcct atcatcagga atacaggact tgagaaaaag agatggaccc cttggctgat aaacgtcttt tgatcaaggc ttcgaccaga tgataaatat ggttccaggc tggaaaatac ggagatggca gtattactac gctcgtgata tttatgat

agtacggcag gaatctgtgg gtagaaaatg aatgatgagt tggggtgatg gacgcccata atgattgttg aaagagcttg cttgatacta ttccgtgctt gatatccttg agagaaaaat aatgatgatg accacccaag tctataggcg aacatatacg catggaagag aagggtctca acctcgtgca aatgttgacg tgcttctcaa aggaacaata ggtttccgtg agatatccat aattaccagg cggataaaac gataagaatg aagcttctga ctacggcctc

agacagtaac acagccattc atggaggctt tagctgatga aggaatgggt tactatgcac ctgtttccta gtggtatcga aacaagtcaa atatttattc tcggaacttc tcccactgga gaaagattga gagtggaaat atgttgatgg ttcttagtgg tgatgaacca ccatcgttac ctgacgttgt gtggagatga cgccttctcc aggccaatcg atgaggaagg ctggttcaca gagagaggag taccaactgt gactccattt aatggcttct aagaagccgt

caaaagtggg ggacagtaaa agaagctgac atacagttat tgaggggcag tcctgtaatt tttcttcgac cattaaaaat gtggatcaaa ttcaccaacg ctttggctta aatggctgaa acttgtaact ttgggaagca tgacggacac caaggatggt acttcttctt tacatccttt tgacattggc tctcgatctt tcaccatccc ttatgatcgt caaaaacttc agcaccctac gataacgcag gggagtgaga ctcagacgaa tgttctcccg gcctctcccg

aggcgacttc gacaacagtg gcagattcat gattatgacg cacgagaact gctgacatag cccgagtact tatattgcta gagctagatt gttgttgatt ttctacgcca attcaaggag actgattcac catcttaaga acggaggttg tctattgtcc gttgatctga gacggttacc gaaacttcat attgtctcaa cttaaggctt gaaggcgttt tgggctgaga aacgttacta agccagatct acaacaggaa ttctcactaa atgctcggga tctttttccc

3240 3300 3360 3420 3480 3540 3600 3660 3720 3780 3840 3900 3960 4020 4080 4140 4200 4260 4320 4380 4440 4500 4560 4620 4680 4740 4800 4860 4920 4938 <213> Artificial sequence <220>

<223> Subject retained 3 <220>

INSECURE (2) .. (2)

Xaa can be any naturally occurring amino acid

<221> <222> <223> <220> <221> <222> <223> <220> <221> <222> <220> <221> <222> <223> <400>

UNSAFE (4}.. (4) Xaa can

INSECURE (7) .. (8) Xaa can

be any naturally occurring amino acid

be any naturally occurring amino acid

VARIANT (9) .. (9)

/ replace = "Glu" 52

Asp Xaa Asp Xaa Asp Gly Xaa Xaa Asp 1 5

<210> <211> <212> <213> <220> <223> <400>

53 36 PRT

Sequence

string 53

To be

Gly Gly Ser 1

Asp Leu Val

Val 20 Leu

artificial

FG-GAP Conserved Domain Name

Val Wing Wing Gly Asp Read Asn Gly Asp Gly Arg Pro 5 10 15

Gly Wing Pro Gly Wing Asp Gly Gly Thr Asp Gly Ser 25 30

Val Tyr Leu 35

<210> 54 <211> 2449 <212> DNA

<213> Arabidopsis thaliana <400> 54

gttctctctg ctctç

attcttcatc gccattgata gatgctctct agaagcatgg accacctatt tgatgccaaa agcacgtgtt acgtgctgtg gaagcaggtt gaaaaagctg gatagcaatt tggtggacgg gacagcacaa ttctggagccggcgcgcg

ggatttgcta tttcatttgt gtagctgatc attcaggttc cttgcggaaa gccatggcca gtggtcgttg tgggaaacga tggataagca atggagtgatc ataaacttgc agtaaaaaga cacaattaca

tattcttcac tcttcagcac

gtttgagtgc agggaattta acgtgaagat acattattga gaagcaggct ggtagtaagt cgctggaaag

ttacctcagg ttggtctgtg atctgcagga ggatttccca attacacatt gaagcatggt agccatataa tcacatggac aacacagaag aagtgctact gtcacttttc tgtctatgca ctgatgatgt tgaagctcac agcttgatgt gcatgctcta gagaatcaat tcttagtgtc agcttgctca tttcaggcga ctacagctta tccgtttcac gaaagattcc aaaattgatt agggtatgca atacattccg

ctacctactg atgcaaatct 60 aaacgcgatt tggctatttt 120 gagggtgatt ttgcgttcaa 180 tacgaagctg atcgtctccc 240 gaggttctcg ttgctactaa 300 gtggatgaag gttttagtga 360 atccgtgttg cgtcagggag 420 tataaaaatg gaactcccca 480 ctctgctttg atcacaacct 540 cataatgcac accatagaga 600 gatacgggtt tggttattgt 660 ccatttgagg aacttggcat 720 gagaatcagg cctctgaaga 780 tttgctggca agactggcct 840 acctcagatg catcacaatt 900 aatagccgtc acccaggaga 960 atgccccatc gctgggaccg 1020 cacaagagga aaacattgaa 1080 aaacctgagg aacacacacc 1140 ggaaaggctg cacgctatgc 1200 acgataacta attacacgaa 1260 gaaggaattg aagctattca 1320 gaaggtggac ttcacgccga 1380 cataaacgga gatggtgttc tcgatcatgt ccagactgtc aactgtagtg agcgggtcaa tggaagtgtt gaagccttgc cgttcccatc cgggaacagc tctttaacgt ctcgatctgc cttgcactat ggaggagatt actcacgaca cttcgcccag ggagatcgca actcccattc tcatccccag agatgatgga tcacggagat gtaatcttct tgacaaaccg tggagaggtg gcacggccac gacgcagtct ggcaatggca gcttcagaca cccgtctcca tcgggtttaa ctgaatcagg gacggtggtc gttgcgcatt catgataacc agcctatgat ccttgcc ggg catctctccg ggaggaagca tattggcttc catcgaatta acttatcact gatgacttct cgaacgatgg tctaacggat tggggtttac gggtttgttc aaaccagaca gccaggggct gggttgtctc ttagtagtta tggcagttat tttcgttact aggtaagcct cgaccatcat ctagctttta atagaaatct tacggaagtt aaaccggcag gtccgcttta gcagcttcac cggaaatatt actgatatac cagtgtagat tcagtgcctt taactttata ctttgtagaa ttcaagtaaa aaatggtaga tttttgtcaa ttttgatgtt ttaatggaat tatgtgacat <210> 55 <211> 698 <212> PRT

<213> Arabidopsis thaliana <400> 55

Met Arg Lys Arg Asp Leu Wing Ile Leu Met Leu 15 10

Phe Phe Thr Read Gln His Glu Gly Asp Phe Wing

20 25

Phe His Tyr Asp Glu Tyr Pro Val Lys Tyr

35 40

Pro Pro Pro Ile Val Wing Asp Read Asn Gly Asp

50 55

Leu Val Wing Thr Asn Asp Wing Lys Ile Gln Val 65 70 75

Arg Arg Val Asp Glu Gly Phe Ser Glu Wing Arg

85 90

Thr Read Leu Pro Asp Lys Ile Arg Val Wing Ser

100 105

Wings Met Wings Thr Gly Val Ile Asp Arg Tyr Tyr

ggaggcaatg tgggcagtgg catcactccc gcaagagaca cacaaacacc acatcataca gaagccacat ccaaccctga ggagatcaag ccgtctcaac gtgattgtga cagcacttaa cgtagagttt ccacatcgggt atttgtatta

ttggagagag caacctcagg cttttaactt cctctactct gcaaaggcag cgcctgatgt

115

120

Gln Lys Gln Val Val Val Val Val Val Ser Gly

130

135

Phe Asp His Asn Leu Lys Lys Leu Trp Glu Thr

145

150

155

Phe Pro His Asn Wing His His Arg Glu Ile Wing

165

170

Tyr Thr Read Lys His Gly Asp Thr Gly Read Leu

180

185

Met Glu Met Gln Pro Tyr Asn His Met Asp Pro

195

200

Met Thr Wing Gln Asn Wing Asp Gln His Arg Arg

210 215

Gln Wing Be Glu Asp Be Gly Wing Ile Asn Leu 225 230 235

Tyr Ala Phe Ala Gly Lys Thr Gly Leu Leu Arg

245 250

Asp Asp Val Glu Wing His Thr Be Asp Wing Wing

260 265

His Asn Tyr Lys Leu Asp Val

275 280

Glu Phe Glu Cys Arg Glu Phe Arg Glu Ser Ile

290 295

His Arg Trp Asp Arg Arg Glu Asp Thr Read Leu 305 310 315

Ser Gly Phe Wing Ile 15 Phe Lys Glu Wing Trp 30 Glu Wing Asp Arg Leu 45 Gly Lys Lys Glu Val 60 Leu Glu Pro His Ser 80 Val Leu Wing Glu Ile 95 Gly Arg Arg Wing Val 110 Lys Asn Gly Thr Pro 125 Trp Ser Val Leu Cys 140 Asn Leu Gln Glu Asp 160 Ile Ser Ile Ser Asn 175 Ile Val Gly Gly Arg 190 Phe Glu Glu Leu Gly 205 Ser Wing Thr Glu Asn 220 Arg His Phe Ser Val 240 Trp Ser Lys Lys Thr 255 Gln Leu Ile Pro Gln 270 Ser Arg His Pro Gly 285 Le Ser Val Val Pro 300 Lys Leu Wing His Phe

1440 1500 1560 1620 1680 1740 1800 1860 1920 1980 2040 2100 2160 2220 2280 2340 2400 2449

320 Arg Arg His Lys Arg Lys Thr Read Lys Lys Gln Wing Gly Ser Lys Ser

325 330 335

Thr Wing Tyr Pro Phe His Lys Glu Glu His Thr Pro Wing Gly Lys

340 345 350

Asp Leu Be Arg Lys Ile Pro Lys Leu Ile Gly Lys Wing Wing Arg Tyr

355 360 365

Gly Wing Be Lys Pro Wing Lys Lys Gly Met Gln Tyr Ile Pro Thr Ile

370 375 380

Thr Asn Tyr Thr Lys Leu Trp Trp Val Pro Asn Val Val Val Wing His 385 390 395 400

Gln Lys Glu Gly Ile Glu Wing Ile His Leu Pro Thr Gly Arg Thr Leu

405 410 415

Cys Lys Read Be Read Read Glu Gly Gly Read His Wing Asp Ile Asn Gly

420 425 430

Asp Gly Val Leu Asp His Val Gln Thr Val Gly Gly Asn Val Gly Glu

435 440 445

Arg Thr Val Val Gly Be Met Glu Val Leu Lys Pro Cys Trp Wing

450 455 460

Val Wing Thr Be Gly Val Pro Ile Arg Glu Gln Leu Phe Asn Val Ser 465 470 475 480

Ile Cys His His Being Pro Phe Asn Phe Read His Tyr Gly Gly Asp Tyr

485 490 495

Be Arg His Phe Wing Gln Wing Arg Asp Thr Be Thr Read Leu Glu Ile Wing

500 505 510

Thr Pro Ile Read Ile Pro Arg Asp Gly His Lys His Arg Lys Gly

515 520 525

Being His Gly Asp Val Ile Phe Leu Thr Asn Arg Gly Glu Val Thr Being

530 535 540

Tyr Thr Pro Asp Val His Gly His Asp Val Wing Trp Gln Trp Gln Leu 545 550 555 560

Gln Thr Glu Wing Thr Trp Being Asn Leu Pro Being Pro Gly Leu Thr

565 570 575

Glu Ser Gly Thr Val Val Pro Thr Read Lys Pro Phe Be Read Le Arg Ile

580 585 590

His Asp Asn Gln Pro Met Ile Read Wing Gly Gly Asp Gln Wing Ala Val

595 600 605

Ile Ile Ser Pro Gly Gly Ser Ile Leu Wing Ser Ile Glu Leu Pro Ser

610 615 620

Gln Pro Thr His Wing Read Ile Thr Asp Asp Phe Ser Asn Asp Gly Leu 625 630 635 640

Thr Asp Val Ile Val Met Thr Be Asn Gly Val Tyr Gly Phe Val Gln

645 650 655

Thr Arg Gln Pro Gly Wing Read Phe Phe Be Ser Be Read Val Gly Cys Leu

660 665 670

Leu Val Val Met Wing Val Ile Phe Val Thr Gln His Leu Asn Ser Ile

675 680 685

Gln Gly Lys Pro Arg Pro Be Ser Be Phe

690 695

<210> 56 <211> 2834 <212> DNA <213> Oryza sativa <400> 56

atgcgtcccc tcctcgcctt cgcggcggta tgcgcccttc tcgtggctgc cgcagcgccg 60

gcggccgcgg aggaggagaa ggcgaacaag ttccggcagc gcgaggccac cgacgacatg 120 cttggatacc cccaccttga tgaagatgct ttattgaaga ccaagtgtcc aaaacatgta 180 gagctgagat ggcagactga agttagttcc agcatttatg caactccgtt gatcgctgat 240 atcaacagcg atggaaagtt ggaagtagtg gtgccatcat ttgttcatta cctggaagtt 300 cttgaaggct ctgatgggga caaattgcca ggatggcctg catttcacca gtcaaatgtt 360 cattcaagtc cacttctata tgatattgac aaggacggga cacgggaaat agttttggca 420 acttacaatg gtgtagtgaa tttcttcagg gtatcaggtt atatgatgat ggacaagcta 480 gaagtacctc gtaggaaggt acacaaagac tggtatgttg ggctgaatac agatcccgtt 540 gaccgctccc atccagatgt tcatgacagc tcaattgcaa agaaagctgc ttctgaagaa 600 tctcacccaa

cggagcacaa

gagccagttg

aatctaaaca

tcacatgttc

gaaactgatg

ctagaggctg

gagtacaatt

aaagaacaac

accccagtga

tacttctttg

gacattggca

aaatggactg

tcttcgccga

tcctttggct

gagatggcgg

gagatggtca

atctgggagg

ggagatggtc

ggaaaggatg

cctgttctat

actacatcct

gttgacattg

gacctcgatc

ccgcaccatc

cgttacaacc

ggcaagcatt

caagctcctt

cgcattgtgg

gttcctgtga

ttctctgacg

gtgcttcttc

gctccgttgc

tcctcataga

gagcttgtcc

atgctgcatc

ttttgtcaca

tctgatcctg

<210> 57

<211> 851

<212> PRT

<213> Oryza

<400> 57

atattcagga atgactcaac aaagtaaacc gcacagatgc agagaaggtt caagtgatac atgctgatgc acgattatga aacatgaaaa ttgcagatat accacgagta aatatattgc cagaacttga ccgtggttga tgttttatgt agattcatgc ctgctgatgt ttcatcttaa gcactgaagt gctcaaaaat tgcttgacat ttgatggtta gagagacctc ttattgttac cacttaagga gtgaaggtat tctgggtaga ataacgtgac ttaatgcagc gaaccacagg agttctcgct caatgcttgg catcattttc agcagataag acatcatact ccatcagtta cttgagattg gttt

sativa

caagccagtt aacacgagga taattctacc tgggaacaac gcttcaaaca cggaacagca atcatttaat tgactacgtt ggcagaagat tgacagagat ttatgataaa aagcagtata tttgagtaca tttggatggt tatcgatcat accagtcatt ccatggcaat gagtcttatc tgtggtccca tcagcctttc gagcaaacat cttgtatttg gtacagtatg taccatgaat atggagatca ttatgttaaa gtttgagatt ggttactcta ttataatgaa aactgtgctt caccttccac gatgtttagc aaggaatatt cagaacaaga gcatcgggga gctagttcgg ttttctaact

gtgaatgaat gttgattcca cgaggacagg tctagtttaa gatgagaaaa aaagcagcta ttgtttcggg gatgaaacca tatgtgagca ggcatacaag ccagaacatc gttgttttta gatagtggaa gatggaaatt cgtggtaagg gcagcagata gtagcagcat ccacagcgcc accgtgtcag ccatatagaa gatgaaaagt attgagggct gttttggctg ggcaatgtct tcaaaccagg cacggttcca gtggacaagt cttgtccctg ccaggcaagc gtggaaatgg atgcattatt gttcttgtca gattagtgat tattagcagg gctcaatctg gaagtacagc tatgatattt

cttctaagga tgaaacatgc agaatatgga gtactacaac gtaatcaagc ctgttgaaaa atgtagaaga tgtggggaga tagatgctca aaatggtgat taaaggagtt accttgacac attttactgc tggatattct ttaggaacaa tcaatgatga ggactgcaga ctactgttgg gaaacatata cgcatggaag ctaaaggcct caagtggctg ataatgttga tctgcttctc gaagaaacaa gaacattccg acagggttcc ggaattatca agcggatgaa ttgacaaaaa acaagcttct tcctgcggcc ttgcacagtg atatccaatc cacagaaaaa ttttagtcat acacaaggaa

atcccagtca gtctaaggaa tgtgttaaac agagaatgca aggaagctca tagcgagcct tctgcctgat cgaggactgg catcttgtcc ttctgtatct aggagggatt aagacaagtc ccatgcatat tgttggaact gttcccactt tgggaaaatc gggagaagaa tgatgttaat tgttcttagt gatcatgagt cacccttgct tgcagatgtt tggtggagat cactccctct tgctgcatat tgacgaagag ttatgggaac aggagaaagg gcttcccaca cgggttctac gaaatggctc acaagaaggc aaacgtcagg acccatgaag acccctcacg gcaaaaagaa ctggcagctg

Met Arg Pro Leu Leu Wing Phe Wing Val Wing Cys Wing Leu Leu Val Wing Wing 1 5 10 15 Wing Wing Pro Wing Wing Wing Wing Glu Glu Glu Lys Wing Asn Lys Phe Arg 20 25 30 Gln Arg Glu Wing Asp Thr Met Leu Gly Tyr Pro His Leu Asp Glu 35 40 45 Asp Ala Leu Leu Lys Thr Lys Cys Pro Lys His Val Glu Leu Arg Trp 50 55 60 Gln Thr Glu Val Ser Ser Ile Tyr Ala Thr Pro Leu Ile Ala Asp 65 70 75 80 Ile Asn Ser Asp Gly Lys Leu Glu Val Val Val Pro Ser Phe Val His 85 90 95 Tyr Leu Glu Val Leu Glu Gly Ser Asp Gly Asp Lys Leu Pro Gly Trp 100 105 110 Pro Wing Phe His Gln Ser Asn Val His Ser Seru Leu Tyr Asp 115 120 125 Ile Asp Lys Asp Gly Thr Arg Glu Ile Val Leu Wing Thr Thr

660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1920 1980 2040 2100 2160 2220 2280 2340 2400 2460 2520 2580 2640 2700 2760 2820 2834

145

150

155

160 Glu Val Pro Arg Arg Lys Val His Asp Trp Tyr Val Gly Leu Asn 165 170 175 Thr Asp Pro Val Asp Arg Be His Pro Asp Val His Asp Be Ser Ile 180 185 190 Wing Lys Lys Wing Wing Be Glu Glu Be His Pro Asn Ile Gln Asp Lys 195 200 205 Pro Val Val Asn Glu Be Ser Lys Glu Be Gln Ser Arg Be Thr Asn 210 215 220 Asp Be Thr Thr Arg Gly Val Glu Be Lys Pro Asn Be Thr Arg Gly Gln Glu Asn Met 245 250 255 Asp Val Leu Asn Asn Leu Asn Be Thr Asp Wing Gly Asn Asn Be Ser 260 265 270 Leu Be Thr Thr Thr Glu Asn Wing Be His Val Gln Arg Arg Leu Leu 275 280 285 Gln Thr Asp Glu Lys Be Asn Gln Wing Gly Be Be Glu Thr Asp Wing 290 295 300 Be Asp Thr Gly Thr Wing Lys Wing Thr Val Glu Asn Be Glu Pro 305 310 315 320 Leu Glu Wing Asp Wing Asp Wing Ser Phe Asn Leu Phe Arg Asp Val Glu 325 330 335 Asp Leu Pro Asp Glu Tyr Asn Tyr Asp Tyr Asp Tyr Asp Tyr Val Asp Glu 340 345 350 Thr Met Trp Gly Asp Glu Asp Trp Lys Gln Gln His Glu Lys Wing 355 360 365 Glu Asp Tyr Val Ser Ile Asp Wing His Ile Read Ser Thr Thr Pro Val Ile 370 375 380 Wing Asp Ile Asp Arg Asp Gly Ile Gln Glu Met Val Ile Ser Val Ser 385 390 395 400 Tyr Phe Phe Asp His Glu Tyr Tyr Asp Lys Pro Glu His Leu Lys Glu 405 410 415 Leu Gly Gly Ile Asp Ile Gly Lys Tyr Ile Wing Be Ser Ile Val Val 420 425 430 Phe Asn Leu Asp Thr Arg Gln Val Lys Trp Thr Wing Glu Leu Asp Leu 435 440 445 Ser Thr Asp Be Gly Asn Phe Thr Wing His Tyr Be Pro Pro 450 450 455 460 Val Val Asp Leu Asp Gly Asp Gly Asn Leu Asp Ile Leu Val Gly Thr 465 470 475 480 Ser Phe Gly Leu Phe Tyr Val Ile Asp His Arg Gly Lys Val Arg Asn 485 490 495 Lys Phe Pro Leu Glu Met Ala Glu Ile His Val Ile Pro Wing 500 Wing Wing 500 505 510 Asp Ile Asn Asp Asp Gly Lys Ile Glu Met Val Thr Wing Asp Val His 515 520 525 Gly Asn Val Wing Trp Wing Wing Trp Glu Gly Glu Glu Ile Trp Wing Glu Val 530 535 540 His Leu Lys Ser Leu Ile Pro Gln Bear Val Gn Asp Val Asn 545 550 555 560 Gly Asp Gly Arg Thr Glu Val Val Val Pro Thr Val Ser Gly Asn Ile 565 570 575 Tyr Val Leu Be Gly Lys Asp Gly Lys Ile Gln Pro Phe Pro Tyr 580 585 590 Arg Thr His Gly Arg Ile Met Ser Pro Val Leu Leu Leu Asp Met Ser 595 600 605 Lys His Asp Glu Lys Ser Lys Gly Leu Thr Leu Wing Thr Thr Be Phe 610 615 620 Asp Gly Tyr Leu Tyr Leu Ile Glu Gly Being Ser Gly Cys Wing Asp Val 625 630 635 640 Val Asp Ile Gly Glu Thr As Tyr Be Met Val Leu Wing Asp Asn Val 645 650 655 Asp Gly Gly Asp Asp As Leu Asp Leu Ile Val Thr Thr Asn Gly Asn 660 665 670

Val Phe Cys Phe Be Pro Pro Be His Pro His Read Lys Glu Trp

675 680 685

Arg Be Ser Asn Gln Gly Arg Asn Asn Wing Wing Tyr Arg Tyr Asn Arg

690 695 700

Glu Gly Ile Tyr Val Lys His Gly Ser Arg Thr Phe Arg Asp Glu Glu 705 710 715 720

Gly Lys His Phe Trp Val Glu Phe Glu Ile Val Asp Lys Tyr Arg Val

725 730 735

Pro Tyr Gly Asn Gln Wing Pro Tyr Asn Val Thr Val Leu Leu Val

740 745 750

Pro Gly Asn Tyr Gln Gly Glu Arg Arg Ile Val Val Asn Wing Tyr Wing

755 760 765

Asn Glu Pro Gly Lys Gln Arg Met Lys Read Pro Thr Val Pro Val Arg

770 775 780

Thr Thr Gly Thr Val Leu Val Glu Met Val Asp Lys Asn Gly Phe Tyr 785 790 795 800

Phe Ser Asp Glu Phe Ser Leu Thr Phe His Met His Tyr Tyr Lys Leu

805 810 815

Leu Lys Trp Leu Val Leu Leu Pro Met Leu Gly Met Phe Ser Val Leu

820 825 830

Val Ile Leu Arg Pro Gln Glu Gly Wing Pro Leu Pro Be Phe Ser Arg

835 840 845

Asn Ile Asp

850 <210> 58 <211> 2411 <212> DNA <213> Oryza sativa <400> 58

gagtgtctag gccagtccag tccagtccag tccaggacgg tccagtccgg tcccgtagag 60

cgccggcgcg tgtagcttcc tcctccgctc caggctccag cgagggaggg agggagacca 120 ccgtctccgc cggctttgag agagaaagag aaagagagag agagagagcg gcgagatgcg 180 gaagcgggat ctgggcatcc tcctcctcgc cgccttcgcc gtcttcttct cgctccagca 240 cgacggcgac ctctccttcc gcgaggcctg gtaccacctc tccgatgccg actaccccat 300 caagcacgac gccgaccgcc tcccctctcc cctagtcgcc gacctcaacg gcgacggcaa 360 acccgaggtc ctcatcccca cccacgacgc caagatccag gtcctccagc cccaccccag 420 gccctccccc gacgatgcct ccttccacga cgcccgcctc atggctgatg tctccctcct 480 cccctccaac gttcgcctct cctccgggag gcgccccgtc gccatggccg ttggcaccgt 540 cgaccgccac tacgcccacg ccccctcccc ctccaagcag ctcctcgtcg tcgtcacctc 600 cggatggtcc gtcatgtgct tcgaccacaa cctcaaaaag ctctgggagg ctaatctcca 660 ggacgatttc ccccacgccg cccaccatcg cgaggttgcc atttccatta ccaactacac 720 ggggatgcag gtttggtcat cgtcggagga tctcaagcat aggatggaaa tgcaacatca 780 ttcagcagag cttttcgatg aatttatggt gtcagaacac aacagggaag agcaccgtag 840 aagcgccagt gagaagcagg cttctgagac aggcaacaca gacctgcgtc attttgccct 900 ttatgctttt gctggccgc the ctggtgaatt aagatggagc cgaaagaatg agaatatccc 960 atcgcaacca tccgatgctt cagtgctgat accacaacac aattacaagc ttgatgccca 1020 tgcccttaat agtcgtcatc ctggtcagtt cgaatgtcgg gaatttagag aatcagttct 1080 tggggtcatg cctcatcatt gggataggag agaggatact tttctgcaac ttgcccattt 1140 taggaggcat aaaaggaaag cactgaagaa aacacctgga aaagctgttg taaataacgt 1200 gcacaagccc agtgaacata atccacctgg aaaggatgtt tccaataggt tagcaaatgt 1260 gattgggaaa gctgcagata tggctaattc aaataaaatc aagaagtcac aaaggacgct 1320 ttatgttccg acaatcacca actatactca agtttggtgg gttcctaatg ttgttgttgc 1380 ccacgaaaaa gaagggatag aggctgttca tctagcttct ggacgtacaa tctgcaagct 1440 tcatttaaca gaaggaggcc ttcatgcaga tattaatgga gatggggttc tagaccatgt 1500 tcaggttgtt ggtgcaaatg gcatcgagca aacagttgtt agtggttcaa tggaagtgct 1560 gaaaccttgt tgggcagtag ctacatctgg tgtgccagtg cgggagcaac tttttaatgt 1620 ttctatctgc cattacaaca atttcaattt gtttcatcat ggtgactttt caagaagttt 1680 tgggaggaca tttgatacaa ctggcttaga ggtggcgact cctattctgc tccagagaga 1740 tgatggtcat aaacacagga gagga agcca cggggatatc atctttctta cgagtcgtgg 1800 ggaggtgacc tcgtactctc caggtctact tggtcatgat gcaatatgga gatggcaact 1860 gtcaacaggt gcaacatggt ccaaccttcc gtctccatca gggatgatgg aaaacattgt 1920 agttccaact ttgaaggctt tctctctgcg agcctatgac ccaaaacagg taatcatcgc 1980 gggtggtgat ctggaggctg tggtgatttc tccttctggt ggtttattgg catccattga 2040 actccctgca cctccaaccc atgcgctggt acttgaagat ttcaatggtg atggtttaac 2100 tgatattatt ctggtaacat cggggggagt ctatgggttt gtgcagacaa gacatcctgg 2160 ggctcttttc ttcagcacgc ttgtgggttg cttgatagtt tgatatttgt gttatcggag 2220 ttcactgcac ctcaactcct cgaacagcgg taaacctagg gcttcaaccg actataggtg 2280 acgacattat gtcagtcgac agaattcctt gtcaatattg aggtgttgtt atagcatttg 2340 atttagattt ttcatataat caactgccaa ctgggttatg taaacttgtg cggcgaatgc 2400 2411 t atttttggcg

<210> 59 <211> 701 <212> PRT

<213> Oryza sativa <400> 59

Met Arg Lys Arg Asp Leu Gly Ile Leu Leu Leu Wing Ala Phe Ala Val 1 5 10 15

Phe Phe Be Read Gln His Asp Gly Asp Read Be Phe Arg Glu Ala Trp

20 25 30

Tyr His Read Being Asp Wing Asp Tyr Pro Ile Lys His Asp Wing Asp Arg

35 40 45

Leu Pro Ser Pro Leu Val Asp Wing Read Asn Gly Asp Gly Lys Pro Glu

50 55 60

Val Leu Ile Pro Thr His Asp Wing Lys Ile Gln Val Leu Gln Pro His 65 70 75 80

Pro Arg Pro Pro Asp Asp Wing Be Phe His Asp Wing Arg Read Le Met

85 90 95

Wing Asp Val Ser Leu Leu Pro Ser Asn Val Arg Leu Ser Ser Gly Arg

100 105 110

Arg Pro Val Wing Met Wing Val Gly Thr Val Asp Arg His Tyr Wing His

115 120 125

Wing Pro Be Pro Be Lys Gln Read Leu Val Val Val Thr Be Gly Trp

130 135 140

Ser Val Met Cys Phe Asp His Asn Read Lys Lys Read Le Trp Glu Wing Asn 145 150 155 160

Read Glu Asp Asp Phe Pro His Wing Ala His His Arg Glu Val Ala Ile

165 170 175

Ser Ile Thr Asn Tyr Thr Read Lys His Gly Asp Wing Gly Leu Val Ile

180 185 190

Val Gly Gly Arg Met Glu Met Gln His His Be Wing Glu Leu Phe Asp

195 200 205

Glu Phe Met Val Be Glu His Asn Arg Glu Glu His Arg Arg Be Wing

210 215 220

Be Glu Lys Gln Wing Be Glu Thr Gly Asn Thr Asp Read Arg His Phe 225 230 235 240

Wing Read Tyr Wing Phe Wing Gly Arg Thr Gly Glu Read Arg Trp Be Arg

245 250 255

Lys Asn Glu Asn Ile Pro To Be Gln Pro To Be Asp Wing To Be Val Leu Ile

260 265 270

Pro Gln His Asn Tyr Lys Read Asp Wing His Wing Read Asn Be Arg His

275 280 285

Pro Gly Gln Phe Glu Cys Arg Glu Phe Arg Glu Ser Val Leu Gly Val

290 295 300

Met Pro His His Trp Asp Arg Arg Glu Asp Thr Phe Leu Gln Leu Wing 305 310 315 320

His Phe Arg Arg His Lys Arg Lys Wing Read Lys Lys Thr Pro Gly Lys

325 330 335

Val Val Wing Asn Asn Val His Lys Pro Be Glu His Asn Pro Gly

340 345 350

Lys Asp Val Ser Asn Arg Leu Wing Asn Val Ile Gly Lys Wing Asp Wing

355 360 365

Met Wing Asn Be Asn Lys Ile Lys Lys Be Gln Arg Thr Read Tyr Val

370 375 380

Pro Thr Ile Thr Asn Tyr Thr Gln Val Trp Trp Val Pro Asn Val Val 385 390 395 400 Val Wing His Glu Lys 405 Glu Gly Ile Glu Wing 410 Val His Leu Wing Ser 415 Gly Arg Thr Ile Cys 420 Lys Leu His Leu Thr 425 Glu Gly Gly Leu His 430 Wing Asp Ile Asn Gly 435 Asp Gly Val Leu Asp 440 His Val Gln Val Val 445 Gly Wing Asn Gly Ile 450 Glu Thr Val Val 455 Ser Gly Ser Met Glu 460 Val Leu Lys Pro Cys Trp Val Wing Ala Thr Ser Gly Val Pro Val Arg Glu Glu Leu Phe 465 470 475 480 Asn Val Ser Ile Cys 485 His Tyr Asn Asn Phe 490 Asn Leu Phe His His 495 Gly Asp Phe Be Arg 500 Ser Phe Gly Arg Thr 505 Phe Asp Thr Thr Gly 510 Leu Glu Val Wing Thr 515 Pro Ile Leu Leu Gln 520 Arg Asp Asp Gly His 525 Lys His Arg Arg Gly 530 Being His Gly Asp Ile 535 Ile Phe Leu Thr Being 540 Arg Gly Glu Val Thr Being Tyr Ser Pro Gly Leu Leu Gly His Asp Wing Ile Trp Arg Trp 545 550 555 560 Gln Leu To Be Thr Gly 565 Wing Thr Trp To Asn 570 Leu Pro To Be Pro 575 Gly Met Met Glu Asn 580 Lys Wing Phe Ser 590 Leu Arg Wing Tyr Asp 595 Pro Lys Gln Val Ile 600 Ile Wing Gly Gly Asp 605 Leu Glu Wing Val Val 610 Ile Ser Pro Gly 615 Gly Leu Leu Wing Ser 620 Ile Glu Leu Pro Wing Pro Pro Thr His Wing Leu Val Leu Glu Asp Phe Asn Gly Asp Gly 625 630 635 640 Leu Thr Asp Ile Ile 645 Leu Val Thr Be Gly 650 Gly Val Tyr Gly Phe 655 Val Gln Thr Arg His 660 Pro Gly Wing Leu Phe 665 Phe Ser Thr Leu Val 670 Gly Cys Leu Ile Val 675 Val Ile Gly Val Ile 680 Phe Val Ser Leu His 685 Leu Asn Ser Ser Asn 690 Ser Gly Lys Pro Arg 695 Wing Ser Thr Asp Tyr 700 Arg

<210> 60 <211> 1103 <212> DNA

<213> Triticum aestivum <220>

<221> misc_feature <222> (16) .. (16) <223> η is a, c, g, or t <400> 60

gcgttccgga gctttnaaaa gagtcaggag ggattgacat aggaccatat attgcatgca 60

gtatagttgt gggtaacctt gacacaaaac aagttaaatg gacagcagaa ctcgatttga 120 gtaccgaaag cgggaaattc cttgcccatg catattcgtc tccaactgtg gttgatttgg 180

atggtgatgg aaatttggat atccttgtcg gaacttccta tggcttgttt tatgttcttg 240 atcatcacgg taagactagg aaaaatttcc cccttgagat ggctgagatc catgcaccag 300

tcattgcagc agacatcaat gatgatggta agatcgagat ggtcactgct gatgtgcatg 360

gtaatgtagc agcttggact gcagagggag acgaaatctg ggaggtgcat ctgaagagcc 420

ttgttccaca gcgacctact gtcggggacg tcaatggaga tggccacact gatgttgtgg 480

tcccaactgt atcaggaaac atctacgttc ttagtggaaa ggatggctca aaagttcagc 540

ctttcccata tagaacacat ggaaggatca tgagtccggt cttgttagtt gacatgagca 600

aacgtggaga aaagacgcaa ggactaaccc ttgctactac gtcctttgat ggttatttgt 660

atttgatcga gggctctagt ggctgtgcag atgttgtcga cattggagag acctcgtaca 720

ctatgggttt ggctgacaat gtggatggcg gagatgacct tgatctgatt gttactacca 780

tgattggcaa tgtcttttgc tttttcactc cctcaccgga acatcctctc aaggaatgga 840

gatcatcaaa ccaggaaagg aataatgctg catataggca ccacccgtca aggtatttat 900

gtaaggcatg gttcaggaca acccgggaga gaaaagggta aacatttcgg ggtcgatttg 960 aaaattggag acaagtacag ggttccctat gggaattaac tccttaaaat gtcaggggaga 1020 cttggggaggggggag

<210> 61 <211> 1152 <212> DNA <213> Zea mays <220>

<221> misc_feature <222> (232) .. (256) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (416). . (474) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (525) .. (529) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (1025) .. (1047) <223> η is a, c, g, or t <400> 61

acaggcttat tataggtttg ttttctacta atagttatga ccgagagttt tgcttctcct ctccgagggt cgcttctctt tcaaaggcct actgtaaact ctcaatggtg acaatatgct attgaaaatg gctgtgtaca aaattgttct gcagaactaa nnnnnnnnnn nnnnnnaaat ggttaactgt tgggatacta tcatggagat gttggggagt cacttcgtca gcttcaatgg agtcgatatt ccgtgaaaac gatggcagcg gcgcaccttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcatgtggaa ggtgagtgag aactcgtcgg agaagtacag ccacgaccac agttccagta gttctaacag gaacagtggg ctggttgatg atacacacca ctgacaacaa tgcgcctatc caagtaaagt taccgtcacg ttataaggag cttgcttccc caacgatttc aaactctatc cagaaattct tgccctcttc catgcttaac atagatacct tcacggttgt actgatatgc ttgatgacct ccattcctta agcggatggt gtggtgattg tgccattcat agtggtaaca ataagatcaa ggtcatctcc aaaccatagt gtacgaggtc tctccaatgt caacaacatc caatnnnnnn nnnnnnnnnn nnnnnnnacg ttgttgcaag ctccatgttt gctcatgtcg agaagtagaa caggactcat atgggaaagg tt <210> 62 <211> 720 <212> DNA

<213> Solanum tuberosum <400> 62

gtttcaatct agataccaag caagttaaat ggactgcaca gttggactta agtactgacg 60

acgggaaatt ccgtgcctat atatactctt ctcctacggt agttgatttg gatggtgatg 120 gaaacatgga cattctagtg gggacctcct atggcttctt ttatgtgttg gatcacaacg 180 gcaaagtgag ggaaaagttc cctctcgaaa tggctgaaat ccaaggagca gtagttgcag 240 ctgatatcaa tgatgatgga aagattgaac tagttacaac agattcacat ggaaatgttg 300 ctgcttggac cgcacaaggc acagaaattt gggaaacgca tctcaagagc cttgttcctc 360 agggaccggt tattggcgat gtggatggag atggccatac agatgtcgtt gtcccaacac 420 tttctgggaa tatatatgtt ctgaatggca aggacggctc atttgtacgt ccatatcctt 480 ataggactca tggtagggtg atgaatcgag cacttcttgt cgacttgagc aaacgtgggg 540 agaagaaaaa agggcttaca attgtcacaa tgtcatttga tggttatttg tatctcatag 600 atggaccaac atcatgtgct gatgttgtag atattggtga aacttcatac agcatggtct 660 tggctgataa tgttgatggt ggcgatgatc ttgatcttat tgtaacaacc atgaatggta 720 <210> 63 <211> 904

agagcgtttt agaatctgac tgttctttgg atgggtacat cactgaacaa gtcaccggca ttggggcctc nnnnnnnnnn ccagnnnna aagcatcatc tccttgataa atagggcacc gtcacggaag aggattcagcagcagcagcagcagcaggag

gacacagtat aattaccgtt gtgagccaat

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1152 <212> DNA <213> Aquilegia <400> 63

gtgaatattg attcacacat cctttgcact cctgtgattg cagacatcga caatgacgga 60

gtatcagaaa tggttgtcgc tgtatcctat ttctttgatc atgagtatta tgacaaccca 120

gagcatctct ctgagttggg tggcatagat attgggaaat atgtagcggg aggaattgta 180

gtatttgatc ttgatacaag acaagttaag tggacaacag agctagatct tagtacagac 240

acaggggact tccgtgctta tatatattct tctccaacgg tggtcgattt ggatggagat 300

ggaaatttgg acattcttgt tgggacatct tttggcttgt tttatgtttt ggaccataat 360

ggcaagataa gaaacaagtt ccctctcgaa atggctgaga ttcagggctc tgtcattgcg 420

gcggatataa atgatgatgg aaaaattgaa ttggttacaa ctgatactca tggaaatgtt 480

gctgcatgga ccccagaagg agaagaaatt tgggaagtac atcttaagag tcttgttcca 540

caacgtccaa cagttggtga tgttgatggc gatggtcata ctgatgtggt ggttcctaca 600

ctatcaggga acatatacgt tctcagtggc aaggatggct cttttgttca cccataccca 660

tatcgtactc atgggagagt catgaatcaa gttcttttag tagatctaag taaacgtgaa 720

gagaaacaga agggactcac tcttgtcaca acatcatttg atggctattt gtaccttatt 780

gacggaccat catcttgtgc tgatgttgtt gatattggcg agacttcata tagtatggtc 840

ttggcagaca atgttgatgg tggggatgat cttgatctaa ttgttacaac tatgaatggg 900

aatg 904 <210> 64 <211> 708 <212> DNA

<213> Brassica napus <400> 64

atttgggaag tgcatctaaa gagtcttgtt ccccagggtc cttcaatagg cgatgttgat 60

ggtgatggac atactgatgt cgtggttcct acaacgtcag gaaacattta tgttcttagt 120

ggcaaggatg gttcgattat acgtccgtac ccgtacagaa ctcatggaag agtgatgaac 180

caacttcttc ttgttgatct gaacaagcga ggtgagaaaa agaaggggct caccattgtt 240

accacatcct ttgacggtta catgtacctc atagatggac ccacctcatg cacggacgct 300

gttgatattg gtgaaacttc atacagcatg gtcttggctg ataatgttga cggtggagat 360

gatcttgatc taatcgtctc aactatgaat ggaaacgtct tttgcttctc tacaccttct 420

cctcaccatc ccctcaaggc gtggagatcg actgatcaag ggaggaacaa taaggcaatc 480

cgttacggtc gtgaaggtgt ctttgtcact cattcaacca gaggcttccg tgacgaggaa 540

ggcaaaaact tctgggctga gatcgagatt gttgataact acagataccc atctggttca 600

caagcaccct acaacgttac tacgacgttg ttggtaccag gcaactacca aggagatagg 660

aggataacac atagccagat ctatgaccga ccaggaaaat acagaatt 708 <210> 65 <211> 834 <212> DNA

<213> Citrus sinensis <400> 65

gttgatgggg atggccatac tgatgttgta gttccaacac tatcggggaa catttacgtt 60

cttagtggca aggatggcag taaagtccgt ccttatcctt atagaactca tggaagggtg 120

atgaatcaag tccttcttgt tgatttaact aaacgcgggg agaaaagcaa gggactcaca 180

attgttacaa catcttttga tggctatttg taccttatag atggcccaac atcatgtgct 240

gatgtagtcg acattggcga aacttcatat agcatggtct tagctgacaa tgttgacggt 300

ggagatgatc ttgatcttat tgttaccaca atgaacggca atgttttttg cttctcaact 360

cctgctccac accatcccct caaggcatgg agatcaatta accaaggaag aaacaatgtt 420

gcaatccgtt acaaccgtga aggaatctat gttacacatc catcaagagc tttccgtgat 480

gaggaaggca gaaacttctg ggtggagatt gagattgtag atgaatatag attcccatct 540

gggtcccaag ctccatataa tgtcactaca acattgttgg tccccggcaa ttaccaaggt 600

gagaggagga ttaagcaaag ccaaatcttt gcacgtcgtg gaaaatatag aatcaaattg 660

ccgacagtcg gggtaaggac gacagggact gttttggtgg agatggttga caagaacgga 720

ctttatttct cagacgaatt ctcgcttaca ttccatatgt attactataa actactaaag 780

tggctcctag tcctcccgat gctcgggatg tttggtgtgc ttgtcatcct tcgt 834 <210> 66 <211> 906 <212> DNA

<213> Asparagus officinalis <400> 66

gggtggaaag gatggatcgt ttgttcgacc cttcccctat aggacacgag ggagattgat 60

gagtccagtt cttctggctg atttaagcaa acgtgatgaa aagttaaagg gcctaactct 120

tgttacaact gcatttgatg gctatttgta cctaattgat gggtccactg gttgtacgga 180 tgttgttgac attggcgaga catcatacac ggacgatctt gaccttattg ttactactat ctcaccgcat catcctctga aggaatggag aagtagatat aaccgtgaag gaatatacat ggaaggtaaa catttctggg tggagctgga gcatcaaggg ccttataatg tcacaacaac aaggcgaatt gtcgtcaaca atgtatataa aacagttcct gttcgaacaa cagggactgt gtatttctct gatgaatttt ctcttacatt gcttctgttt ctaccgatgc tcggaatgtt aggtgcaccg ttaccatcgt tttcacgaaa gaagatgaga atactccaaa gtgcgatgct tgtgtg <210> 67 <211 > 779 <212> DNA <213> Populus <220>

<221> misc_feature <222> (34) .. (39) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (663) .. (663) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (686) .. (688) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (739) .. (744) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (746) .. (748) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (760) .. (761) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (766Y .. (769) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (773T .. (777) <223> η is a, c, g, or t <400> 67

tgaatcaagt tcttcttctt gacttaagta cttgttacaa catcattcga tggttatctt gatgttgttg atattggtga aacttcatat ggagatgatc ttgatctcat agtttcaaca cctgttccac atcatcccct gaaggcttgg gcaaaccgct acaaccgtga aggggtgtat gaggagggga agagcttctg ggtggaattt gggtctcaag caccttataa tgtcactaca gaacgacgga taaagcaaaa tcaaatcttt ccaacagttg gagtgagaac tactggaact ctctatttct cagatgactt ctcgcttaca tgnctccttg tcctcccaat gcttgnnntg gaggccgtgc ccttgccann nnnntnnngg

catggtcttg gaatggcaat atcatccaac atcacatgga gataatagac attattagtt tcaacctgga gctggtggag tcacatgcat tggagttctt tttggattga atctgacatg

gcagataatg gtcttttgct cagggaagaa tccagagctt aagtacaggt cctggaaatt aagcaacgga atggttgaca tactacaagc gtcattctac tattaatgat tatgcctttg

ttgatggtgg tttccacacc acaatgcagc tccgcgatga ttccctcagg accaaggaga taaagctgcc agaatggatt tactgaagtg gtccccagga atcatccaat tacatctgaa

aacnnnnnng taccttatag agcatggtct atgaatggaa agatctaata attaaacctt gagattgtgg accctgttag gaccgtccag gttttggtgg ttccacatgc tttggtgtgc aatactgacn

agaaaaacaa atggaccaac tggcagacaa atgtcttttg atcaaggaag catcaagaag acaagtatag ttcctggcaa gaaaatatcg agatggttga attactataa ttgtcatcct ngtgannnnc

gggactcaca ttcttgtgct tgttgatggt cttttcaact aaacaatgta tttccgtgat aatcccgtct ttatcaaggt gataaaactt taagaatgga actgctgaag tcgtccacaa atnnnnnac

240 300 360 420 480 540 600 660 720 780 840 900 906

60 120 180 240 300 360 420 480 540 600 660 720 779 <210> 68 <211> 723 <212> DNA <213> Populus <400> 68

ggcacgagca cacttgttac aacatcattt acttcttgtg ctgatgttgt tgatattggt aatgttgatg gtggagatga tctagatctc tgcttttcaa ctcctgttcc acatcaccct agaaacaacg tggtgaaccg ctacaaccgt agttttcgtg atgaggaggg aaagagcttc agattcccat ctgggtctca agcaccttat aattatcaag gtgagagacg aataaagcaa cgggtaaaac ttccaacagt tggagtgagg gataagaacg ggctctattt ctcagatgac aaactgctga agtggctcct agtcctccca cttcgtccac agaggccatg ccccttacat tct

<210> 69 <211> 704 <212> DNA

<213> Euphorbia esula <220>

<221> misc_feature <222> (10) .. (11) <223> is not a, c, g, or t <400> 69

ctgatacccn ntgaaatgtt gctgcttgga atctcaaaag tcttatttcc cagggcccat ccgatgtggt agtccctact atatctggga caaatgttcg cccataccca tatagaaccc ttgatttaag taaacgtggg gagaaaagca atggatactt gtaccttata gatgggccca aaacctcata tagcatggtc ttagcggaca tagtcacaac aatgaatggg aacgtctttt ttaaggcatg gagatcggct aatcaaggta aaggggtcta tgttacacct tcatcaagag gggtggaaat cgaaatcgtg gataaatata aagtcactac aaccctgtta gttccgggta <210> 70 <211> 1088 <212> DNA

<213> Ceratopteris richardii <400> 70

ccacgcgtcc gggttacaac agatgcccgg aaagaaattt gggaagttca cttaaaaagc attgatggag acggaacatt ggatattgtt ttgcatggaa agactggtgt tactatgaag atggctcctg ttcttctggt ggacttggcc ctggtggttt catcatttga tgggtacctc gatgtggttg atgtaggaga gacatcctac ggagatgatt tggatttaat agtgacgacc cctgctcctt atcatccttt acggtcatgg gcatctcgta taagacatga agggatttat gaaggtggtg aaagtttttg ggtgcatttc gggccttaca atattacggc tacccttctt attacagaga gtcagttaat tcaacaacca tctgtacgat catctgggac tgtagttcta actgatgagt attcattgac attccacatg gtgcttccaa tgcttggaat gctttttgtc tcagttcttc cttccttttc aagggatcat ctcctaaatc caaaccttat tgtaaatgta gaaatgca

gatggttatt tgtaccttat agacggacca 60 gaaacttcat acagcatggt cttggcagat 120 180 atagtctcaa caatgaatgg gaatgtcttt ctcaaggctt ggagatcttc taatcaagga 240 300 gaaggggttt atgttacacc ttcatcaaga tgggtggaat ttgagattgt agacaagtat 360 aatgtcacta caaccctttt agttcctggc 420 480 agccaaatct ttgaccgtcc aggaaattat actactggaa ctgttttggt ggagatggtt 540 600 ttctccctta cgtttcacat gcattactat atgcttggaa tgttttgtgt gcttgtcatc 660 720 723 cattttcagg aatactgact tgtgatatca

cttcacaagg cagttggtga atatatatgt atgggagagt ccggactcag catcctgcgc atgtagacgg gcttcgcgac gaaataatgt cttttcgaga gatacccttc attatcaagg

aaaggaaatt tgtggatggg tttgagcggc aatgaatcaa ccttgtaaca tgatgtcgtt aggagatgac tcctgttcca ggcaaacaga cgaggaagga tggttctcaa tgag

tgggagaggc gatggccata aaggatgggt gttctccttg acttcatttg gatattggtg cttgacctag catcatcccc tttaaccgtg aagaatttct gcaccttata

ggaaatgttg ttgattgctc gtgccaactg ccatatccat aagctaggca tacctcatag acaatggtgc atgaatggga acctcacaga gctacatctg aatattattg gtaccccaca ggaacgcaca gagatggtgg cactattatgg tacgatgga

ctgcatggac agggtccgac tgtcaggaaa ttcgtaccca ccgagagaag atggtcccac ttgcagacaa atgttttctg atcagggtag gctcgagaag atgagaaccg actacatggg agttcaaact ataaacatgg gggtctgtctctg

aaataaagga tgtcggagat tatatatgtt tgggcgtgtg aggtctggtc agcctgtgct cgttgatgga cttctctaca gagcaatctt tttcagggat cttaccttcg tccaaggcgc gcggggggggggggggggggggggggggg

60 120 180 240 300 360 420 480 540 600 660 704

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1088 ctctgcgctt tcgctatttt cttctctctt actttctcac tcttaaaccc taaccctcat

<210> 71 <211> 830 <212> DNA

<213> Welwitschia mirabilis <400> 71

atgacaatca tgaacacttg aaggagctgg gtgatattga tattagcaaa tatgtttcag gtgctatagt ggtatttaat cttgatacaa agcaagtgaa gtggagtact cagttggatc ttagcacaac ctctgggact tttaatgcat acatctattc ttctcctact gtggttgacc tggatggtga cggcaatttg gatattattg ttggcacgtc atttggtttc ttttatgtat tggatcacca tggaaaaaat agagaaggct ttccattaca gatgggtgag attcaaggac aagtaattgc tgctgatata aatgatgatg ggaagattga aatggttaca acagataccc gtggaaatgt ggctgcttgg acttctcagg ggaaagaaat atgggagata catttaaaaa gtcttattgc tcaggggcct acagtaggtg atattgatgg agatggtcat acagatttgg tggttcctac agtatcagga aatatttatg tattaaatgg gaaagatgga tcattggtga agccatttcc ttatcgtact catggaagag tcatgagtcc ggtacttttg gttgacctta gcaaacgtgg ggaaaagcaa aagggcttaa ctcttgcagc attatcattt gatggttact tctacttaat tgatggtcaa acagcttgtg cagatgttgt tgatattgga gagacttcct actctatggc tttggcggac aatgtagatg gcggcgatga tcttgatttt attatcacaa ctatgaatgg caatgtgttc tgcttttcaa cccctgctcc acatcatcca <210> 72 <211> 8730 <212> DNA <213> Medicago sativa <400> 72

atgaggaagc gtgatttggc gattcttatg caggtattgt accctttaac cccctaattc tttcaattca attgattcag caagatggtg gtgtttcatt caaagacgcg tggatgcatc taacagatga atacccaatc aaatacgaag ccgaacgcct tcctcctcct gttgtcgccg atctcaacgg cgatggtaag aaagaagttc tcgtagctac ccacgatgcc aaaattcagg ttctttcatt tcccaatttt cctcaatttt ttattcactt ataaaattcc aggttttatt tggtttcctt cacaactgga cattgtcaaa aaaacatttt ttttctttca gtaaaccatc aatgtagttg atgaactata actctcttca atttagtttg taagtagtct ctaaaattca tcaattaatc cctgctatta atgtttggtt ctacaccgaa gaaagttgac tttgaatgag ttgattgtgt aaactctatt tgggctaaaa ttgatttgaa ggtaaagtga tttatgtttg gactttggat acattcatgt aaaagtgaat tgaacaggtg aaaaatcaac tctagaatca gaagctacaa tttctaactt taagtagaat gtagaatcaa ttctggaggt aaaatcaatt ttactctaga agaaccaaat atgtcaatca attttgggtc ctccaaaatt agaaccaaac atacagaaag tagagactaa aattgatgga ttttcatata ctttagggac ttaattgacc ggttttatat actttaaaga ctacttatca tatttatata gtttagggac tagattgatg gtacaagtaa tatagtagta actggatttt gtatttatgg tcggtgtctt agattttgga gccacatagt aggcgtgttg atgaaggatt cagtgaggca cgtgtgttag cggaggtgtc tctgttgcct gacaaagtgc gtgtcatgtc tgggagacga cctgttgcta tggccactgg ctttattgac cgccatagaa ttggacaacc acataagcag gttttggttg tagtaacatc gggttggttt gtaatgtgtt ttgattccaa cctccaaaag ttgtgggaaa ataatttgca ggtatgatgt ttatgttgtg ccatgtttca gttgatagaa ttttcagtta aacattttga ttatcttgtt atgttttagt ttaccaactg ttttagtggt tgttaacagg aggatttccc ccataatgct caccacaggg aagtttcaat atctataagc aattatactc tcaagcatgg agatacagga ttgatcattg ttggtgggag aatggaaatg cagcctcatg tatgtgtcac tttccttctt gttttctggt ttgttattct ttttagcaga gtatgtgtgt ctgtgttcta tctgctgatg gaaaaactta gaatttaatg tggatggtgt ttaccatctt ttatgaaagc gaaaactctc aacttcatga gtagcataag attctggcta tcttgttttc ttggaagtga tgaaaatgaa agttcctgag taattttatt tgataaagta tgatggtaat gtgagggttt gctgctgctt ctcgagtttc agaatttttt atttgttatt tgattctttc tatggtaatg attttggaat tgttcatctt tggaattatt ggcattatct tctaatctga tggttctgtt ggcttatgtt gattgctttc cattttcaga tttttatgga cccttttgaa gaaatgggaa tgggagctag atttgctga g cagcatcgaa gaagtgctac tgaaaaggag gtatagatac gtttctagtt gatgctaaat tttatgccca tttttttact atctgtagca tgctaatttt ggtttaaact ttgagacact atttgcttta tttatttcac tttaattttg ggtcttcaag gttgacctta ttttatgctc ctaaacagtc aattcaaatt cctattttga tataaataga taatataaaa gttcaccatg tcttggcatg ataaagccaa taaataaaat ctgtgcacct tgcagtaagt agtctataaa tctgaacctt catgatcagg tagctaaata aacaggagac agtttcactc gttttgttta gatttttgtt tttacaaaaa aatgtatcag gtgattttag ttttcttccc attttaaggt tgaagacttt aacttgttta ttgtgttcct ttttctggat

60 120 180 240 300 360 420 480 540 600 660 720 780 830

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1620 1680 1740 1800 1860 1920 1980 2040 2100 2160 2220 2280 2340 aggcttctga aaacaccgga actgtggatt tacgccattt tgcattttat gcatttgt

gtcgatcagg tgtagaacga tggagcagaa aaacagaggt ggtgttcttt tttttttgtt 24 60

ttcagtgcgc tctattattt gggtgttgtc aacatgcaaa tatgaaacaa ttatttattt 2520

ttaatttaaa aatgcatttt cttgtttttt aaaagagctg tcttttctat ctggaaattc 2580

attaggtgat taagtgaact ttctctgtcc tccctctctg tacataactt tattcagggg 2640

gtagcaactt agttctagac agtatgtttt gtcctgcaac agacatgaaa actgaagaaa 2700

agttatatag ttaagctcac aaagttcaga acataatgag ttagagccta aatgtcaata 2760

atagattata aattatacct ttgaagatat tggagtcttg ttgcaagcat ctgcacttgg 2820

attttaacgc actggtgcac aaatatgtat tttttgaaaa gaaaatctat caaacaaatg 2880

gaaccacctc ccaaccagca taaactttag tcttttctgt ctttgggtgg tagtaccaga 2940

tagatgtttt acaatcaaca tagcggtcca gtttgattga atatgtgatt tacgctagtc 3000

caacatttat tttgttaaat gatcttgtgt atccttgtcc tttttttttt cttttctttt 3060

ctaattgcta gtattgtgat ttaattgttt agaacattga agcagcagct tcttcagacg 3120

catcacagtt aattccacag cataactaca aacttgatgt tcatgctctg aatagacgtc 3180

aacctggaga ggtacttagc tatagctgtt caatttaatc ctaaattcct aatggtatat 3240

tgaattgatg gaatatggta aattggagtg gcataaaata ccattccatc ctttttaagc 3300

aggggaatca ttgcttttct ttttcataca tccattttat tctaatctag aaatatgcat 3360

gttgattttt atgttctccc ctttagctca acgtactgat tttgttatat gtaagtaata 3420

ctgtaatttc agatttatta ctttttccat tcctttcctc atcaatctta agtaataccg 3480

tagaatagat tcctagtcat atctttatgt tcttcacctt ttctcttcaa atgatcaaac 3540

actaaagcaa atatttgttt gttaatatgg agtttactat gagaactcta gtacaatagt 3600

actcatccaa atctacttga gtaacttggt catctccttc ctttgtgtgt ggaaatagca 3660

gcatgtgcac ataaaggagt gtttcaatca aatttaatat tattgtgaaa cctttgtata 3720

tgtttatcca tatctaccca atagatagct tcctgtgaca tgtgaaatta tagtttgaat 3780

gcagggaatt cagagaatca atcctgggag ttatgccaca tcaatgggta cactctattt 3840

tcaaccttga ttaacgtttg gtactaaagc ataatctcaa atgtcatagt taactgatat 3900

tagatacatg cactcatcag gataggagag aagatacttt attgaagttg gtccacttca 3960

atcggcataa gaggaaaaca ttgaagaaaa cacctggaaa gactatcaat taccctttcg 4020

acaagcctga ggaaaaccat ccaccaggga aggactcaac caaaaaaatt tcaaacataa 4080

ttgggaaagc agcaaatttt gctggttcag caaaatccaa gaaggtaatt agaaaccatg 4140

tcatgcaatg caactggtaa caattcattc aattgttgac ataatgataa ttagttttaa 4200

gatggctaca actggacaac tcaaaaattc ttttccaaac atgatttcag ctacaagctt 4260

ttgttgtctt tatccagtaa cagagctgtc tgtttacttt ctaactactg catgtccaat 4320

tttttgttat gtgatctaaa tctttgtgtt ggtaatattc tcagcatctg ctgattttaa 4380

ttcattttgg ctaaacctat tttgaatttg ttatgataac cattgcttag catttttaat 4440

ttttttttta aactaagcac tggtcagata aaccatctca cttgttatat ttgtatttgt 4500

tcttaaagat ttaagtctag ctacattttt tatttctcat ttaaattttt tgaatcctga 4560

atttagggag ccaagatatt tagttataaa aataatgatt aataataaat atgcagttct 4620

ccccattgtc gtgttaattt ggtagatagc atagtagtca atcttggata gcggtgcgta 4680

gtggccaacc ccaaaaatgg gatagcgtat agcggtatga caaatagcgg tcaccgatgt 4740

ttgattattt tttggacaat tatatgaata atagcaaagt ataaacaaac ttatatattt 4800

ctcaacaaaa aaaacttata ttaaatataa cactcaaatc tcacgagaca ttcataaatc 4860

aaaacaccga aaacaaaaga caggaacaaa taaaaaaaat cataaaacag gagaaaccat 4 920

aagatagaag atagaagagt gtgtttcgcg gcacaggtga gaagagagaa aaagttgaaa 4 980

aatgttaggg tttacgaaat cctcaaaatg ccctaaaaaa ggttttaaaa atagggtcaa 5040

tttggaaatt tcctactaaa ataaatagcg gctgctatcc aacccgcacc gctatagcag 5100

ctgaagcggc cgctatttga ttccgcgcta tttgataccg ctatgctaca cgcggccgct 5160

atagcgccac tatcggctgc tattgactac tatggtagat agaaactgtt aggttaccta 5220

gtagggctga tgatgtgtta gtgggctcaa gtaatgagtg agaggcccat tatttggagg 5280

gtgtatgaac gtgggaagaa gagaggagag aagggactaa gggtgcttcc tagctggcag 5340

ttagttagta tgaggcagag ggagtgaata tatttcttct gaggagctgt tgctctagtt 5400

tatccgtcat agtgatttca ttgttattct agtttagcca ggctttggga gtatttggta 5460

tatctacctc gaagatgcta ggtcctaaaa tgatgcagtt ggaaggttcg attgcgggag 5520

tctctctgtt ggtgatgatt taaacacaat ttcatatact cctggtgtgg ttgaagcgtt 5580

gggggttgcc tttggattca tcagatactg ctggtgtgca tttgggtggg ggtggggggt 5640

catatggtgc agatattggg caaatgtgtg gttggcttct gcatatcggc ccttatgcag 5700

tcttgctgga tacatatgtg ttggggttag aagaggtcat gccaaagtac caattactct 5760

cttaatacct gtctaataag actctaatgc tttaaattga cgaggatttg catttttcac 5820

ctttcactgg caaacatatg ggttaaactg gtagatgttt agcgtgttca agtacaagct 5880

gtgttcccat agatgaagta cttcatgttt ggggagtttg atcgttaaac tacctaattg 5940

tttgtttaaa tgaattttga attccctctt ctaataattt tagtgcttcc aaagattata 6000

atgcatataa aacaatttga ggattatctt gttcttaggt gaaaaacagt ttttttccca 6060

tttgaaatta gataaacaga gtagttgtag atttctactg tggaaagcat aacatgtcta 6120 agctggttaa atatacttaa ttgatggttt ttgtcattaa agtaatttct ttgcatacat 6180

ttttatgttc tcatgttatt gcttaatcgg aaaggggttc tataaaatgg atggcatttt 6240

atgggattcc gagaagcctt tttctttttt ttgccagggt ggggggtttg cattatttgt 6300

cataatgttt tttcttttgg aatgcagtat ccaccatatg ttcccaccat aaccaactat 6360

actaaggttt ggtgggttcc taatgttgtt gtggctcatc taaaggaggg gatagaagtc 6420

cttcatctgg catctggtcg aacactatgt aaggtaaata taaagccact gatcagcttg 6480

aaaatcaaaa tacaaaaaaa aaaaaaaaaa gtttacaagt agttgtacaa catttgctcc 6540

tgtggttgta gatttgtagt attaaactgc catggtttca gccaaaatct tatttagcat 6600

tcacgttact tttgaaattt ttcgtattct gtgaaatgac acttctattt atctcagctt 6660

caccttcagg aaggtggtct acatgctgat attaatggtg atggagttct ggatcatgtg 6720

caggttcact ttttttcctt cacatttttt gataaacaaa caagccaaat ctttattaat 6780

ttagcatgat aattgttaac ttaacggggg attttcctct gcctgatgtc tgaaagattt 6840

atctaatcag gctgttggag gaaatggtgc tgagcaaact gtagttagtg ggtccatgga 6900

tgttctacgt ccttgttggg ctgttgcaac gtctggcgta ccagtacggg aacaactctt 6960

caatgtatct atttgtcatt atacccattt taacttattc caacatggag aactttatag 7020

aggcttcaac cgaggttcag atatgtcttc tttggaggta gcaacaccca ttctcattcc 7080

tagaagcgat ggtcacaagc accggaaggg aagccacggg gatgttatct tcttgacaaa 7140

ccggggagag gtacgctctt ttttgttcga ctaaatctgg ttatgtaaca tttgatagtt 7200

tgtttgagca ccaatcaaac aaacctagtt cttaacttct gttaacaaat tatttcaagt 7260

cacgcacgac taatatattt tggtggtatt tgtgagataa tccgtacctc cgtactgact 7320

ttatgaaaat tgctccttgc agataacttc gcacactcct ggtttgcatg gtcatgatgc 7380

tgtttggcag tggcaacaat caactggtgt cacatggtca aacctacctt ccccagcagg 7440

gatgatggaa ggtggtttgg tgattcccac actaaagcct tttcctttgc ggttgcatga 7500

caatcatgag atgatccttg cagctggaga acaagaggct gtggtaatat cacccggagg 7560

tagcatattg gctacaattg aactccctgg ttcacctaca catgtattga tccgtgaaga 7620

cttctcaaat gatgggctca ctgaccttat tctcgtcacc tcatccggag tgtacggctt 7680

tgtccagact cgacaacctg gtgctctctt cttcagtgtg ctgatcggct gtctcatagt 7740

cgtgatggga attatatttg ttacccagca cataaattcc atgaagggga agcctcgtcc 7800

atcatctggt cctaggtgaa atgcaggacc tgaaatttat acattaactt tgcaggcgct 7860

tacagatgaa gcagaagaca aagcggtgtg cgcatcgtat ggagcttcaa gattgttgga 7920

aatgcataga atgtaaggag attttgagga ggtgttgtac tgtctaggat ggatgattgt 7980

aaaatttaga cagaagtgac agaatgtcct tcatgagaga aacatgatcc attaatataa 8040

ggaaactata gtctttgcct acgtatctgg ctgttgttta gaagttatac tgcccacact 8100

tgacaattgt cacccgttcc gaattagttg atacttgata gttgatgcat aacagaaact 8160

caccttggtc tttacacttt tcgctaaaag gattttctct tgtcattaca caattcttgt 8220

aaatacgacc atcgtttatt tgcttcagat ttagcagtgt aaaacattaa atctagcaac 8280

caacttgctc caatctgtct tggtcttctt cagaatacac ttcttaaaag tcaattaaga 8340

aatgacttcc tgattccatc aaattaccaa tgtggttgat cccatcatac atcaagtgct 8400

atgtacgtaa tccctgttag tctttggcat caccttaagt gttttagcta ttgcttttcc 8460

tgtgcgtcga gaaatgtttc aaataaccat ctgaatgatt caatattgca tcatataata 8520

ttgcaaaccc aaaattagtg tgttcctata atggataaag cttgaaaata atgcgagtgg 8580

tcgattagtg cgattggtgc aatgttggat tcattaacaa ctggtattga aaatattgca 8640

taaagctatc aacttctcca tataccatgc tacattgtct tttgcctgat gatgattttt 8700

gacatccaac cgtgtaaata caatattagt 8730 <210> 73 <211> 57 <212> PRT

<213> Medicago sativa <400> 73

Ile Gln Gln Asp Gly Gly Val Ser Phe Lys Asp Wing Trp Met His Leu 1 5 10 15

Thr Asp Glu Tyr Pro Ile Lys Tyr Glu Wing Glu Arg Leu Pro Pro

20 25 30

Val Val Wing Asp Leu Asn Gly Asp Gly Lys Lys Glu Val Leu Val Ala

35 40 45

Thr His Asp Wing Lys Ile Gln Val Leu

50 55

<210> 74 <211> 25 <212> PRT

<213> Medicago sativa <400> 74

Leu Ser Gln Leu His Leu Gln Glu Gly Gly Leu His Wing Asp Ile Asn 1 5 10 15 Gly Asp Gly Val 20 Leu Asp His Val Gln 25 <210> 75 <211> 107 <212> PRT <213> Medicago sativa <400 > 75 Gln Wing Val Gly Gly Asn Gly Wing Glu Gln Thr Val Val Gly Ser 1 5 10 15 Met Asp Val Leu 20 Arg Pro Cys Trp Wing 25 Val Wing Thr Ser Gly 30 Val Pro Val Glu Leu Phe Asn Val Ser Ile Cys His Tyr Thr His Phe 35 40 45 Asn Leu Phe Gln His Gly Glu Leu Tyr Arg Gly Phe Asn Arg Gly Ser 50 55 60 Asp Met Be Ser Leu Glu Val Wing Thr Pro Gly His Lys His 85 Arg Lys Gly Be His 90 Gly Asp Val Ile Phe 95 Read Thr Asn Arg Gly 100 Glu Val Arg Be Phe 105 Read Phe <210> 76 <211> 156 <212> PRT <213> Medicago sativa <400 > 76 Gln Ile Thr Be His Thr Pro Gly Read His Gly His Asp Wing Val Trp 1 5 10 15 Gln Trp Gln Gln 20 Be Thr Gly Val Thr 25 Trp Be Asn Leu Pro 30 Be Pro Wing Gly Met Met Glu Gly Gly Leu Val Ile Pro Thr Read Lys Pro Phe 35 40 45 Pro Read Arg Read His Asp Asn His Glu Met Ile Leu Wing Wing Gly Glu 50 55 60 Gln Glu Wing Val Val Ile Ser Pro Gly Gly Ser Ile Leu Wing Thr Ile 65 70 75 80 Glu Leu Pro Gly Ser 85 Pro Thr His Val Leu 90 Ile Arg Glu Asp Phe 95 Ser Asn Asp Gly Leu 100 Thr Asp Leu Ile Leu 105 Val Thr Be Ser Gly 110 Val Tyr Gly Phe Val Gln Thr Arg Gln Pro Gly Wing Leu Phe Phe Ser Val Leu 115 120 125 Ile Gly Cys Leu Ile Val Val Met Gly Ile Ile Phe Val Thr Gln His 130 135 140 Ile Asn Be Met Lys Gly Lys Pro Arg Pro Ser 145 145 155 155

<210> 77

<211> 1521

<212> DNA

<213> Oryza sativa

<400> 77

atggccgcca tgatggcgtc cataaccagc gagctgctct tctttctccc cttcatcctc 60

cttgccctgc tcacgttcta caccaccacc gtggccaaat gccacggcgg gcactggtgg 120

cgaggtggga cgacgccggc gaagaggaag cggatgaacc tgccgcccgg cgccgccggg 180

tggccgctcg tcggcgagac gttcggctac ctccgcgccc accccgccac ctccgtcggc 240

cgcttcatgg agcagcacat cgcacggtac gggaagatat accggtcgag cctgttcggg 300

gagcggacgg tggtgtcggc ggacgcgggg ctcaaccggt acatcctgca gaacgagggg 360

aggctgttcg agtgcagcta cccgcgcagc atcggcggca tcctgggcaa gtggtccatg 420

ctggtcctcg tcggggaccc gcaccgcgag atgcgcgcca tctccctcaa cttcctctcc 480

tccgtccgcc tccgcgccgt cctcctcccc gaggtcgagc gccacaccct cctcgtcctc 540

cgcgcctggc ccccttcctc caccttctcc gctcagcacc aagccaagaa gttcacgttc 600

aacctgatgg cgaagaacat aatgagcatg gacccggggg aggaagagac ggagcggctg 660 cggcgggagt acatcacctt catgaagggc gtggtctccg cgccgctcaa acgccctact ggaaggctct caagtcgcgt gctgccattc tcggagtaat atggaagagc gggttgagaa gctgagcaag gaggatgcaa gcgtagagca ctcggatggg ctctgaaaca atctaacctt tcaaaagagc aaatcctgga agcttgctct tcgccgggca cgagacgtcg tccatggcgc tcgccctcgc cttgaaggct gccccaaggc tgtccaagaa ctgagggagg agcatcttgg agacaaaggc taagagggga gtgcaaattg agctgggaag actacaaaga acgcaatgtg tcataaacga gacgttgcgg ctaggaaacg tggtcaggtt aaggtcatca aggacgtgca ctacaagggt tatgacattc caagcggatg ccggtgttag ccgcggtgca tctggactcg tccctgtacg aggaccccca ccctggagat ggaagagtag cggatcatcc ggcggcttgg ctcagagcag ccgtacggcg gcgggacgcg gctgtgcgcc gggtcggagc tcgcgaagct gtgttcttgc accacctggt gctcaacttc aggtgggagc tcgccgagcc ttcgtcttcc ccttcgtcga cttccccaag ggccttccca ttagggttca caggatgatg the agcaggagta <210> 78 <211> 505 <212> PRT

<213> Oryza sativa <400> 78

Met Wing Met Wing Met Wing Be Ile Thr Be Glu Leu Leu 15 10

Pro Phe Ile Leu Leu Wing Leu Leu Thr Phe Tyr Thr Thr

20 25

Lys Cys Gly Gly Gly His Trp Arg Gly Gly Thr Thr

35 40 45

Arg Lys Arg Met Asn Leu Pro Pro Gly Wing Gly Wing Trp

50 55 60

Gly Glu Thr Phe Gly Tyr Read Arg Wing His Pro Wing Thr 65 70 75

Arg Phe Met Glu Gln His Ile Wing Arg Tyr Gly Lys Ile

85 90

Ser Leu Phe Gly Glu Arg Thr Val Val Ser Wing Asp Wing

100 105

Arg Tyr Ile Read Gln Asn Glu Gly Arg Read Le Phe Glu Cys 115 120 125

Arg Ser Ile Gly Gly Ile Leu Gly Lys Trp Ser Met Leu

130 135 140

Gly Asp Pro His Arg Glu Met Arg Wing Ile Ser Leu Asn 145 150 155

Ser Val Arg Leu Arg Wing Val Leu Leu Pro Glu Val Glu

165 170

Leu Leu Val Leu Arg Wing Trp Pro Pro Be Ser Thr Phe

180 185

His Gln Wing Lys Lys Phe Thr Phe Asn Read Met Wing Lys 195 200 205

Be Met Asp Pro Gly Glu Glu Glu Thr Glu Arg Read Leu Arg

210 215 220

Ile Thr Phe Met Lys Gly Val Val Ser Pro Wing Read Asn 225 230 235

Thr Pro Tyr Trp Lys Wing Read Lys Ser Arg Wing Wing Ile

245 250

Ile Glu Arg Lys Met Glu Glu Arg Val Glu Lys Leu Ser

260 265

Wing Ser Val Glu Gln Asp Asp Leu Leu Gly Trp Wing Leu 275 280 285

Asn Leu Ser Lys Glu Gln Ile Leu Asp Leu Leu Ser

290 295 300

Wing Gly His Glu Thr Be Being Met Wing Leu Wing Leu Wing 305 310 315

Leu Glu Gly Cys Pro Lys Wing Val Gln Glu Leu Arg Glu

325 330

Gly Ile Arg Wing Arg Arg Gln Arg Read Arg Gly Glu Cys Lys

cctgcccggg agagaggaaa agacgatctt cctcttgctg catcttcttc gattgcaagg gatggttttc cctgcaccgg gaagatcctg gcgcttcaat cagcttcatg ggagatggcc ggaccaagcc tagaattgca

Phe Phe Leu 15

Thr Val Wing 30

Pro Wing Lys

Pro Leu Val

Ser Val Gly 80

Tyr Arg Ser 95

Gly Leu Asn 110

Be tyr pro

Val Leu Val

Phe Leu Ser 160

Arg His Thr 175

Ser Wing Gln 190

Asn Ile Met

Arg Glu Tyr

Read Pro Gly 240

Read Gly Val

255 Lys Glu Asp 270

Lys Gln Ser

Leu Leu Phe

Ile Phe Phe 320

Glu His Leu 335

Read Ser Trp

720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1521 340 345

Glu Asp Tyr Lys Glu Met Val Phe Thr Gln Cys

355 360

Leu Arg Leu Gly Asn Val Val Arg Phe Leu His

370 375

Asp Val His Tyr Lys Gly Tyr Asp Ile Pro Ser 385 390 395

Pro Val Leu Wing Wing Val His Leu Asp Ser Ser

405 410

Gln Arg Phe Asn Pro Trp Arg Trp Lys Ser Ser

420 425

Read Wing Gln Ser Ser Ser Phe Met Pro Tyr Gly

435 440

Cys Wing Gly Ser Glu Leu Wing Lys Leu Glu Met

450 455

His Leu Val Leu Asn Phe Arg Trp Glu Leu Wing 465 470 475

Phe Val Phe Pro Phe Val Asp Phe Pro Lys Gly

485 490

His Arg Ile Wing Gln Asp Asp Glu Gln 500 505

<210> 79 <211> 1542 <212> DNA

<213> Arabidopsis thaliana <400> 79

atgttcgaaa cagagcatca tactctctta cctcttcttc cttcttctct tcttgattct cttgaagaga agaaatagaa ccgggtaaat ccggttggcc atttcttggt gaaaccatcg gccacaacac tcggtgactt catgcaacaa catgtctcca tcgaacttgt ttggagaacc aacgatcgta tcagctgatg ttacaaaacg aaggaaggct ctttgaatgt agttatccta gggaaatggt cgatgcttgt tcttgttggt gacatgcata cttaacttct taagtcacgc acgtcttaga actattctac actttgtttg ttcttgattc ttggcaacaa aactctattt aaaaagttta cgtttaatct aatggcgaag catataatga gaaacagagc aattaaagaa agagtatgta actttcatga ctaaatctac caggaactgc ttatcataaa gctcttcagt ttcattgaga ggaaaatgga agagagaaaa ttggatatca gaagaagtga aaacagagga tgaagcagag atgagtaaga agaacagacg atgatctttt gggatgggtt ttgaaacatt attctcgatc tcattcttag tttgttattt gccggacatg gctctcgcta tcttcttctt gcaagcttgc cctaaagccg catcttgaga tcgcgagggc caagaaggaa ctaggagagt tacaagaaaa tggactttac tcaatgtgtt ataaatgaaa gttaggtttt tgcatcgcaa agcactcaaa gatgttcggt agtgggtgga aagtgttacc ggtgatctca gccgtacatt caacctaatc tctttaatcc ttggagatgg caacagcaaa ggaagtggta gtttttcgac gtggggaaac aactacatgc ctatgtgctg gttcagagct agccaagtta gaaatggcag cttaaattca attgggaatt agcagaagat gatcaaccat tttcctaacg gtttgcctat tagggtttct cgtattctgt <210> 80 <211> 513 <212>

<213> Arabidopsis thaliana <400> 80

Met Phe Glu Thr Glu His His Leu Leu Pro 1 5 10

Ser Leu Leu Ser Leu Leu Leu Phe Leu Ile Leu

20 25

Arg Lys Thr Arg Phe Asn Pro Le Pro Gly Lys 35 40

350 Val Ile Asn Glu Thr 365 Arg Lys Val Ile Lys 380 Gly Trp Lys Ile Leu 400 Leu Tyr Glu Asp Pro 415 Gly Ser Ser Gly Gly 430 Gly Gly Thr Arg Leu 445 Wing Val Phe Leu His 460 Glu Pro Asp Gln Wing 480 Leu For Ile Arg Val

495

ttctcccatc aaaccagatt gttatcttaa agtatggtaa ctggacttaa gaagtatagg gagatatgag ttaaagatgt tctctgctca gtatggatcc aaggagttgt cacgagcaac aggaagaaga gtgatcatgt cgaatttatc agacttcttc ttgaagagct cagaattaaa ctcttcgatt acaaaggata tggataattc acaacggagc cgtttggagg tgtttattca aa ttgcttttcc

gcttttgtct caatctacct accgtacacc gatatataga tagattcata tgggattctt aagtatctcg tgagagacat agacgaggcc tggagaagaa ctctgctcct gatattgaag tcaagaagaa taggaaacaa gacggagcaa tgtagccatt tagggaagag ttgggatgat gggaaatgta cgatatccct tcgttatgac gtcatcgtca agggccaagg tcatctagtt ttttgttgat

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1542

Leu Leu Leu Leu Pro 15

Read Lys Arg Arg Asn 30

Ser Gly Trp Pro Phe 45 Leu Gly Glu Thr Ile Gly Tyr Leu Lys Pro Tyr Thr Wing Thr Thr Leu

50 55 60

Gly Asp Phe Met Gln Gln His Val Being Lys Tyr Gly Lys Ile Tyr Arg 65 70 75 80

Ser Asn Leu Phe Gly Glu Pro Thr Ile Val Ser As Asa Wing Gly Leu

85 90 95

Asn Arg Phe Ile Leu Gln Asn Glu Gly Arg Leu Phe Glu Cys Ser Tyr

100 105 110

Pro Arg Be Ile Gly Gly Ile Leu Gly Lys Trp Ser Met Leu Val Leu

115 120 125

Val Gly Asp Met His Arg Asp Met Arg Ser Ile Ser Leu Asn Phe Leu

130 135 140

Be His Wing Arg Leu Arg Thr Ile Leu Read Lys Asp Val Glu Arg His 145 150 155 160

Thr Leu Phe Val Leu Asp Ser Trp Gln Gln Asn Ser Ile Phe Ser Ala

165 170 175

Gln Asp Glu Wing Lys Lys Phe Thr Phe Asn Leu Met Wing Lys His Ile

180 185 190

Met Ser Met Asp Pro Gly Glu Glu Glu Thr Glu Glu Leu Lys Lys Glu

195 200 205

Tyr Val Thr Phe Met Lys Gly Val Val Ser Wing Pro Leu Asn Leu Pro

210 215 220

Gly Thr Wing Tyr His Lys Wing Read Gln Be Arg Wing Thr Ile Read Lys 225 230 235 240

Phe Ile Glu Arg Lys Met Glu Glu Arg Lys Read Asp Ile Lys Glu Glu

245 250 255

Asp Gln Glu Glu Glu Glu Val Lys Thr Glu Asp Glu Wing Glu Met Ser

260 265 270

Lys Be Asp His Val Arg Lys Gln Arg Thr Asp Asp Asp Read Leu Gly

275 280 285

Val Leu Trp His Lys Be Asn Leu Be Thr Glu Gln Ile Leu Asp Leu

290 295 300

Ile Leu Be Leu Leu Phe Ala Gly His Glu Thr Be Val Ala Ile 305 310 315 320

Wing Leu Wing Ile Phe Phe Leu Gln Wing Cys Pro Lys Wing Val Glu Glu

325 330 335

Leu Arg Glu Glu His Leu Glu Ile Wing Arg Wing Lys Lys Glu Leu Gly

340 345 350

Glu Ser Glu Read Asn Trp Asp Asp Tyr Lys Lys Met Asp Phe Thr Gln

355 360 365

Cys Val Ile Asn Glu Thr Leu Arg Leu Gly Asn Val Val Arg Phe Leu

370 375 380

His Arg Lys Wing Read Lys Asp Val Arg Tyr Lys Gly Tyr Asp Ile Pro 385 390 395 400

Ser Gly Trp Lys Val Leu Pro Val Ile Ser Wing Val His Leu Asp Asn

405 410 415

Be Arg Tyr Asp Gln Pro Asn Read Phe Asn Pro Trp Arg Trp Gln Gln

420 425 430

Gln Asn Asn Gly Wing Be Be Be Gly Be Gly Be Phe Be Thr Trp

435 440 445

Gly Asn Asn Tyr Met Pro Phe Gly Gly Gly Gly Pro Arg Read Cys Wing Gly

450 455 460

Ser Glu Leu Wing Lys Leu Glu Met Wing Val Phe Ile His His Leu Val 465 470 475 480

Leu Lys Phe Asn Trp Glu Leu Wing Glu Asp Asp Gln Pro Phe Ala Phe

485 490 495

Pro Phe Val Asp Phe Pro Asn Gly Leu Pro Ile Arg Val Ser Arg Ile 500 505 510

Read

<210> 81

<211> 1590

<212> DNA

<213> Saccharum officinarum <220>

<221> misc_feature <222> (394) .. (394) <223> η is a, c, g, or t <400> 81

atgggcgcca tgatggcctc ctggccctcc tcgccctgta cgccggccga agaagaagcg

ggcgagacct cagcatgtcg gtgtcggcgg tgcagctacc ggcgacgcgc cgcgccgtgc ccctccgacg gcgaagaaca tacatcacct tggaaggcgc aggcttcaga gccctgaagc ttcgccgggc tgccccaagg ctaagagggg tgtataaacg caagatgtgc gcggcggtgc tggaagagca gggtcggagc cggtgggagc ggcctcccga cgagaagcac gagggaattg <210> 82

tcggctacct cacggtacgg acgcggggct cgcgcagcat accgcgagat tgctcccgga gcacggtctc taatgagcat

cataaccagc gagctcctct tcttccttcc cttcatcctg caccaccact gtcgccaaat gcgacggcac ccaccagtgg gccgaacctg cccccgggcg ccctcggatg gcctttcgtc ccgcgcccac ccggccacct ccgtgggcct cttcatggag caagatatac cggtcgagcc tgttcgggga gcggacggtg caaccgctac atcctgcaga acgaggggcg gctgttcgag cggcggcatc ctgngcaagt ggtccatgct ggtgctggtg gcgcgccatc tcgctcaact ttctcagctc ggtggagcgc cacaccctgc tggtgctccg cgcgcagcac caagccaaga agttcacgtt

ggaccccggc gaggaggaga cggagcggct

tcatgaaggg cgtcgtgtca gcgccgctca acttcccggg tcaagtcacg cgcgtccata cttggagtaa tagagaggaa aaatgagtaa ggagaactca agtgtggagg aagacgatct

agtccaatct gtcaaaggaa acgagacttc gtcaatggcg

cagatcctgg acctcttgct ctagccctcg ccatcttctt

cgtccgcctc ctcatggccg taacctgatg gcggctggag cacggcctac gatggaggac tcttggatgg gagcctgctc ccttgaagga

ctgttcaaga actccgggag gagcatctcg agattgctag gagacaaagg cgttcaaatt gagctgggaa gactacaagg aaatggtttt cacgccatgg agacattgcg ggttggcaac gtggtcaggt tcctgcaccg gaagggcat

actacaatgg gtacgacata atctggattc gtcgctgtac

ccacgcgggt ggaaaatcct gccggtgtta aaggacccct accggttcaa cccttggaga

acgcgccgag cagcttcatg ccgtacggcg gcgggccgcg gctgtgcgcc

tggccaagct ggagatcgcc tggcggagcc ggaccaagcc tcagggtcca gcggatcgcc gatgtagcgg tacgggtaca gggctgggtg gttatggtaa

atcttcctgc accacctggt gctcaacttc ttcgtctacc ccttcgtcga cttccccaag gacgaccagg gggcatcgca gcgttttgac caagcaagta caaaattttc gttgcatttt

<211> <212> <213> <220> <221> <222>

529 PRT

Saccharum officinarum

INSECURE (132) .. (132)

<223> Xaa can be any naturally occurring amino acid <400> 82

To be Ile Thr

Met 1

Gly Wing

Met Met 5

Allah

Ser Glu Leu Leu 10

Phe

Phe 15

Read

Pro Phe Ile Leu 20 Leu Wing Leu Leu Wing 25 Leu Tyr Thr Thr Thr 30 Val Wing Lys Cys Asp 35 Gly Thr His Gln Trp 40 Arg Arg Lys Lys 45 Lys Arg Pro Asn Leu 50 Pro Gly Wing Leu 55 Gly Trp Pro Phe Val 60 Gly Glu Thr Phe Gly Tyr Leu Arg Wing His Pro Pro Wing Thr Be Val Gly Leu Phe Met Glu 65 70 75 80 Gln His Val Wing Arg 85 Tyr Gly Lys Ile Tyr 90 Arg Be Being Read Le Phe 95 Gly Glu Arg Thr Val 100 Val Ser Wing Asp Wing 105 Gly Leu Asn Arg Tyr 110 Ile Leu Gln Asn Glu Gly Arg Leu Phe Glu Cys Ser Tyr Pro Arg Ser Ile Gly 115 120 125 Gly Ile 130 Leu Xaa Lys Trp Ser 135 Met Leu Val Leu Val 140 Gly Asp Wing His Arg Glu Met Arg Wing Ile Be Leu Asn Phe Leu Be Ser Val Arg Leu 145 150 155 160 Arg Wing Val Leu Leu 165 Pro Glu Val Glu Arg 170 His Thr Leu Leu Val 175 Leu

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1560 1590 Arg Be Trp Pro Pro Be Asp Gly Thr Val Be Wing Gln His Gln Wing 180 185 190 Lys Lys Phe Thr Phe Asn Leu Met Ala Lys Asn Ile Met Being Met Asp 195 200 205 Pro Gly Glu Glu Glu Thr Glu Arg Leu Arg Leu Glu Tyr Ile Thr Phe 210 215 220 Met Lys Gly Val Val Sera Pro Leu Asn Phe Pro Gly Thr Ala Tyr 225 230 235 240 Trp Lys Wing Leu Lys Be Arg Wing Be Ile Leu Gly Val Ile Glu Arg 245 250 255 Lys Met Glu Asp Arg Leu Gln Lys Met Be Lys Glu Asn Be Ser Val 260 265 270 Glu Glu Asp Asp Leu Gly Trp Wing Leu Lys Gln Ser Asn Leu Ser 275 280 285 Lys Glu Gln Ile Leu Asp Leu Leu Read Leu Leu Phe Wing Gly His 290 295 300 Glu Thr Be Met Wing Leu Wing Alle Ile Phe Phe Leu Glu Gly 305 310 315 320 Cys Pro Lys Val Wing Glu Glu Read Le Arg Glu Glu 330 335 Arg Arg Gln Arg Read Arg Gly Ala Phe Lys Read Ser Trp Glu Asp Tyr 340 345 350 Lys Glu Met Val Phe Thr Pro Cp Ile Asn Glu Thr Leu Arg Val 355 360 365 Gly Asn Val Val Arg Phe Read His Arg Lys Val Ile Gln Asp Val His 370 375 380 Tyr Asn Gly Tyr Asp Ile Pro Arg Gly Trp Lys Ile Leu Pro Val Leu 385 390 395 400 Wing Ala Val His Leu Asp Ser Be Leu Tyr Lys Asp Pro Tyr Arg Phe 405 410 415 Asn Pro Trp Arg Trp Lys Be Asn Wing Pro Be Ser Phe Met Pro Tyr 420 425 430 Gly Gly Gly Pro Arg Leu Cys Wing Gly Be Glu Leu Wing Lys Leu Glu 435 440 445 Ile Wing Ile Phe Read His His Leu Val Leu Asn Phe Arg Trp Glu Leu 450 455 460 Wing Glu Pro Asp Gln Wing Phe Val Tyr Pro Phe Val Asp Phe Pro Lys 465 470 475 480 Gly Leu Pro Ile Arg Val Gln Arg Ile Wing Asp Gln Gly Wing Ser 485 490 495 Gln Arg Phe Asp Arg Glu Wing Arg Cys Ser Gly Thr Gly Thr Gln Wing 500 505 510 Be Thr Lys Phe Be Read His Phe Glu Gly Ile Gly Wing Gly Trp Leu 515 520 525 Trp

<210> 83 <211> 1437 <212> DNA <213> Allium strains <400> 83

atggagatca tattagtgtc tacattgatc atatcgctac taatatttct tggatttaga 60

agcaatggga aaacggagag aaaattgctg cctacactac caccaggcaa tcttggaggt 120

tggccattca tcggtgacac cattccgttc atgacacctc attcttctgc tttgttgggc 180

acttacatcg atcaaaatat ttccaaatat gggaggatat ttcgaatgaa cttgttagga 240

aaggcaacga tcgtgtctgt agaccctgat ttcaacagat atattctaca gaatgaagga 300

agattgtttg aaaatagctg cccaacgagc attaaagaga ttttgggaaa atggtctatg 360

cttgcattag ctggggatat acacagagaa atgagatcca ttgctgtgaa tttcatgaac 420

agtgttaagc ttagaactta ttttttaaag gatattgata ttcaggctgt taatattctt 480

gatgcttgga aggtcaactc tactttctct gcacaggatg aaggaaagaa gtttgcattt 540

aacctcatgg tgaagcatct aatgaatatg gatcctggaa tgccagagac agaagaaatc 600

agaaaagagt acattttctt catggagggg atggcttcca ttcctttaaa ctttcctgga 660

acagcctaca gaagagcttt acagtcaagg tccaggattc tggcaataat ggggcaaaag 720

cttgacgaaa ggatgcagaa aataaaagaa ggctgtaaag gactggaaga agaggatctt 780 cttgcctcag ttgcaagaaa tactaacata acaagagacc agattcttga tttgatgatc 840 agcatgcttt ttgctggtca tgaaacttct tctgccgcta tttctcttgc catttatttc 900 ctgcaagctt cccccgatgt tcttaaaaag cttcgagagg agcacataaa gattgcaaaa 960 caaaagaaag aaaggggcga aactgaattg aactgggatg attacaagca aatggaattc 1020 acaaactgcg ttattcatga aaccctaaga ttaggcaaca tcgttaagtt tttgcatcgg 1080 aaaaccatca aagatgttca atacaaaggt tatgaaattc catgtgggtg ggaagtagtg 1140 ccaatcatct cagcagcaca tttggattct tctatctttg acaacccaaa agttatgaat 1200 ccttcgaggt gggaggcgat attttcagca ggagcaaaga gcaatataat gtcattcagc 1260 ggtggacctc ggttatgtcc aggagcagag ttggcgaaac tggagatggc tatttttctt 1320 catcatcttg tacagaggtt cgactgggaa ttggtggaga aggataaccc tgtatcattc 1380 cccttccttg gatttcccaa gaaattgcct atcaaaatca cagctcttaa 1437 acattga

<210> 84 <211> 478 <212> PRT <213> Allium cepa <400> 84

Met Glu Ile Ile Leu Val Ser Thr Leu Ile Ile Ser Leu Leu Ile Phe 1 5 10 15

Read Gly Phe Arg Be Asn Gly Lys Thr Glu Arg Lys Read Leu Pro Thr

20 25 30

Leu Pro Gly Asn Leu Pro Gly Gly Trp Pro Phe Ile Gly Asp Thr Ile

35 40 45

Pro Phe Met Thr Pro His Being Ala Read Leu Gly Thr Tyr Ile Asp

50 55 60

Gln Asn Ile Be Lys Tyr Gly Arg Ile Phe Arg Met Asn Read Leu Gly 65 70 75 80

Lys Wing Thr Ile Val Ser Val Asp Pro Asp Phe Asn Arg Tyr Ile Leu

85 90 95

Gln Asn Glu Gly Arg Read Le Phe Glu Asn Be Cys Pro Thr Be Ile Lys

100 105 110

Glu Ile Leu Gly Lys Trp Ser Met Leu Wing Leu Wing Gly Asp Ile His

115 120 125

Arg Glu Met Arg Ser Ile Wing Val Asn Phe Met Asn Ser Val Lys Leu

130 135 140

Arg Thr Tyr Phe Leu Lys Asp Ile Asp Ile Gln Wing Val Asn Ile Leu 145 150 155 160

Asp Wing Trp Lys Val Asn Be Thr Phe Be Wing Gln Asp Glu Gly Lys

165 170 175

Lys Phe Ala Phe Asn Leu Met Val Lys His Leu Met Asn Met Asp Pro

180 185 190

Gly Met Pro Glu Thr Glu Glu Ile Arg Lys Glu Tyr Ile Phe Phe Met

195 200 205

Glu Gly Met Wing Ser Ile Pro Read Asn Phe Pro Gly Thr Wing Tyr Arg

210 215 220

Arg Wing Read Gln Be Arg Be Arg Ile Read Wing Ile Met Gly Gln Lys 225 230 235 240

Read Asp Glu Arg Met Gln Lys Ile Lys Glu Gly Cys Lys Gly Leu Glu

245 250 255

Glu Glu Asp Leu Leu Wing Ser Val Wing Arg Wing Asn Thr Asn Ile Thr Arg

260 265 270

Asp Gln Ile Leu Asp Leu Met Ile Ser Met Leu Phe Ala Gly His Glu

275 280 285

Thr Ser Ser Ala Wing Ile Ser Leu Ile Wing Tyr Phe Leu Gln Ala Ser

290 295 300

Pro Asp Val Leu Lys Lys Leu Arg Glu Glu His Ile Lys Ile Wing Lys 305 310 315 320

Gln Lys Lys Glu Arg Gly Glu Thr Glu Read Asn Trp Asp Asp Tyr Lys

325 330 335

Gln Met Glu Phe Asn Cys Val Ile His Glu Thr Read Leu Arg Leu Gly

340 345 350

Asn Ile Val Lys Phe Read His Arg Lys Thr Ile Lys Asp Val Gln Tyr

355 360 365

Lys Gly Tyr Glu Ile Ile Cys Gly Trp Glu Ile Val Val Ile Ile Ser 370 375 380

Wing Wing His Leu Asp Ser Ser Ile Phe Asp Asn

385 390 395

Pro Ser Arg Trp Glu Wing Ile Phe Ser Wing Gly

405 410

Met Be Phe Be Gly Gly Pro Arg Read Cys Pro

420 425

Leu Glu Met Wing Ile Phe Leu His His Leu

Lys

435

440

Trp Glu Leu Val Glu Lys Asp Asn Pro Val Ser

450

455

Phe Pro Lys Lys Leu Pro Ile Lys Ile Thr Wing 465 470 475

<210> 85 <211> 1491 <212> DNA

<213> Zinnia elegans <400> 85

atgtgttcaa caaccctaaa tatgtgtgac cttgagttct gtcttggctc tttttctcat cttgaagctt gtcaaaagaa cgaaatcttc caccgggcaa catgggctgg ccgttcatcg caaccgtatt cggctacaac catcggcaag tttatggaac aagatataca aatctagttt gtttggtgag ccaacaatag aataagtaca tattgcaaaa tgaagggagg ttatttgaat gggggtattc ttggcaaatg gtccatgttg gttttggttg aggcaaattt cactcaactt tttgtccaat gcaaggctta gttgagaaaa atactttgtg ggtattagat tcttggaaag caagaagaag ccaagaagtt tacttttaat ctaatggcaa ccgggtgaac cggagaccga gcgattgaag aaagagtatg gtttctcccc ctttaaactt ccctggaact gcatactgga acgattctta aattcatcga aacaaaaatg gaggagcgga ggattaggga aactagacaa tgatcttctt ggatggtcta aaagagcaaa tactcgattt ggtattgagt ctactctttg gtttcgatat cgttagccgt ttacttcctt gaagcttgtc agagaagaac atgaagaaat tgtgatgaaa aaaaagctat tgggatgact acaaaaagat ggagtttact cagtgtgtga gggaatgtgg tgagattcct ccacagaaag gctattaaag gacattccat gtggttggaa agtgctgcca gtgattgcag cattttgacc aaccttacct ttttgatcca tggagatggc tctacttgtt caaccccgcc atcagcaagt aacttcatgc ttatgcacag ggtcagagct agcgaaacta gagatggcga ctaaaatacg agtgggaatt ggttgactca gatgaagcat tttccaaaag

<213> Zinnia elegans <400> 86

380

Pro Lys Val Met Asn 400

Lys Ser Asn Ile Wing 415

Gly Wing Glu Leu Wing 430

Val Gln Arg Phe Asp 445

Phe Pro Phe Leu Gly 460

Read Lys His

tcatccttgc gaacaaacaa gtgaaaccat aacacatatc tttctgctga gtagttatcc gtgacatgca aaactcaact aaaactcacc cacatatcat taactttcat aagctttaaa ttaggatgga tgaagaactc ctggtcacga ctaccgcggt tgggtgagaa tcaatgagac atgtgaggta ccgtgcattt agaacgcaag catttggtgg tatttatcca tcgcttatcc aatcatgtta

atcttgtctt tggttcgact cggttacctc caagtatggg tccagggttg aagaagcata tagagacatg agtaaatgaa tttttgtgcc gagtttagac gaaaggtgtg gtctcgagcg cgaaggaaac aaatctcact aacgtcttcg tcgtcaactt gtatctcact gctaagattc taaaggatat ggatcctaca tgtcacgtca agggccccgc ccatttggtc atatctcgac

g

Met Cys Be Thr Thr Read Asn Met Cys Asp Read Glu Phe Phe Ile Leu 1 5 10 15 Wing Be Cys Read Leu Val Leu Wing Leu Phe Leu Ile Leu Val Lys 20 25 30 Arg Arg Asn As As Gly Be Thr Arg Asn Leu Gly Pro Pro Gly Asn Met 35 40 45 Gly Trp Pro Phe Ile Gly Glu Thr Ile Gly Tyr Leu Gln Pro Tyr Ser 50 55 60 Wing Thr Thr Ile Gly Lys Phe Met Glu His Ile Ser Lys Tyr Gly 65 70 75 80 Lys Ile Tyr Lys Ser Serve Phe Gly Glu Pro Thr Ile Val Ser Ala 85 90 95 Asp Pro Ser Gly Leu Asn Lys Tyr Ile Read Gln Asn Glu Gly Arg Leu Phe 100 105 110 Glu Cys Ser Tyr Pro Arg Ser Ile Gly Ile Leu Gly Lys Trp To be

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1491 115 120 125 Met Leu Val Leu Val Gly Asp Met His Arg Asp Met Arg Gln Ile Ser 130 135 140 Leu Asn Phe Leu Ser Asn Arg Wing Leu Lys Thr Gln Leu Val Asn Glu 145 150 155 160 Val Glu Lys Asn Thr Leu Trp Val Leu Asp Ser Trp Lys Glu Asn Ser 165 170 175 Pro Phe Cys Ala Gln Glu Ala Lys Lys Phe Thr Phe Asn Leu Met 180 185 190 Wing Thr His Ile Met Ser Read Asp Pro Gly Glu Pro Glu Thr Glu Arg 195 200 205 Tyr Trp Lys Wing Leu Lys Ser Arg Wing 225 230 235 240 Thr Ile Leu Lys Phe Ile Glu Thr Lys Met Glu Glu Arg Ile Arg Met 245 250 255 Asp Glu Gly Asn Gly Leu Gly Lys Leu Asp Asn Leu Gly Trp 260 265 270 Be Met Lys Asn Be Asn Leu Thr Lys Glu Gln Ile Leu Asp Le u Val 275 280 285 Leu Ser Leu Leu Phe Ala Gly His Glu Thr Ser Ser Val Val Ile Ser 290 295 300 Leu Ala Val Tyr Phe Leu Glu Cys Pro Thr Wing Val Arg Gln Leu 305 310 315 320 Arg Glu Glu His Glu Glu Ile Val Met Lys Lys Lys Leu Leu Gly Glu 325 330 335 Lys Tyr Leu Thr Trp Asp Asp Tyr Lys Met Glu Phe Thr Gln Cys 340 345 350 Val Ile Asn Glu Thr Leu Arg Phe Gly Asn Val Val Arg Phe Leu His 355 360 365 Arg Lys Ile Wing Lys Asp Val Arg Tyr Lys Gly Tyr Asp Ile Pro Cys 370 375 380 Gly Trp Lys Val Leu Pro Val Ile Wing Val His Leu Asp Pro Thr 385 390 395 400 His Phe Asp Gln Pro Tyr Leu Phe Asp Pro Trp Arg Trp Gln Asn Wing 405 410 415 Ser Val Thr Ser Ser Thr Cys Ser Thr Ser Pro Pro Ser Ser Ser Asn Phe 420 425 430 Met Pro Ser Ser Ply Gly Gly Gly Pro Arg Leu Cys Thr Gly Ser Alu 435 440 445 Lys Leu Glu Met Wing Ile Phe Ile His His 455 460 Trp Glu Leu Val Asp Ser Asp Glu Wing Phe Wing Tyr Pro Tyr Leu Asp 465 470 475 480 Phe Pro Lys Gly Leu Pro Ile Lys Ile Arg His Arg Lys Gln Ser Cys 485 490 495

<210> 87 <211> 1422 <212> DNA

<213> Medicago trunculata <400> 87

atgtctaact catacttaac ttgcagtttt ctttcttcca tctttgttct ttctttgatt 60

ttcattttca tcaaaagaaa gaaaacaagg tataatcttc cacctggaaa aatgggatgg 120

ccctttatag gagaaaccat tggttatttg aagccttaca ctgccaccac aatgggagaa 180

tttatggaaa atcacatagc aaggtatggg acaatttaca agtcaaattt gtttggaggg 240

ccagctattg tatcagcaga tgcagaattg aataggttca tattacaaaa tgatggaaaa 300

ttgtttgagt gtagctatcc aaaaagcatt ggtggaatac ttggaaaatg gtcaatgttg 360

gttttagtag gtgacatgca tagggaaatg aggaatatat cactaaactt tatgagctat 420

gctaggctta aaacacattt tttgaaagat atggagaagc ataccctttt tgttctaagc 480

tcttggaaag aaaattgtac attttcagct caagatgaag caaaaaagtt caccttcaat 540

ttgatggcca aacaaatcat gagtttggat ccagggaatc ttgagacaga acagttgaaa 600

aaagagtatg tctgtttcat gaaaggtgtt gtttctgctc ctttgaattt gccaggaact 660 gcatacagaa aagcattaaa gtctaggaac aatatattga agttcataga ggggaaaatg 720 gaagaaaggg tgaagagaaa ccaagaagga aaaaaaggga tggaggaaaa tgatcttcta 780 aattgggttt taaagcattc aaatctttcc actgagcaaa ttcttgactt gattctaagt 840 ttactttttg ctggccatga aacttcatct gtggctatag ctctagctat ttactttttg 900 cctagttgtc ctcaagctat acaacaatta agggaagagc atagagaaat agctagatcc 960 aagaagaaag caggggaggt tgaattaact tgggatgatt ataaaagaat ggaatttact 1020 cattgtgttg tgaatgaaac ggtaatgttg tgagattcct tcacaggaag actaaggttg 1080 gctatcaaag atgttcatta caaaggttat gacattccat gtggatggaa agtccttccg 1140 gtgatttcag cggtacattt ggatccttca aattttgacc aacctcaaca cttcaatcct 1200 tggagatggc agatgagcaa taacaacttc atgccatttg gaggaggacc aaggctatgt 1260 gcaggattag aattagccaa acttgaaatg gctgttttca ttcaccatat catcctcaaa 1320 tacaactggg acatggtcga tgttgatcaa cctattgtat acccttttgt tgattttccc 1380 aaaggtttgc caattagagt ccaaagccaa gccactcttt aa 1422

<210> 88 <211> 480 <212> PRT

<213> Medicago trunculata <400> 88

Met Ser Asn Ser Tyr Leu Thr Cys Ser Phe Le Ser Ser Ile Phe Val 15 10 15

Read It Be Read It Ile Phe Ile Phe Ile Lys Arg Lys Thr Arg Tyr Asn

20 25 30

Read Pro Gly Lys Met Gly Trp Pro Phe Ile Gly Glu Thr Ile Gly

35 40 45

Tyr Leu Lys Pro Tyr Thr Wing Thr Thr Met Gly Glu Phe Met Glu Asn

50 55 60

His Ile Wing Arg Tyr Gly Thr Ile Tyr Lys Ser Asn Leu Phe Gly Gly 65 70 75 80

Pro Wing Ile Val Ser Wing Asp Wing Glu Leu Asn Arg Phe Ile Leu Gln

85 90 95

Asn Asp Gly Lys Leu Phe Glu Cys Ser Tyr Pro Lys Ser Ile Gly Gly

100 105 110

Ile Leu Gly Lys Trp Being Met Leu Val Leu Val Gly Asp Met His Arg

115 120 125

Glu Met Arg Asn Ile Be Read Asn Phe Met Be Tyr Wing Arg Read Le Lys

130 135 140

Thr His Phe Leu Lys Asp Met Glu Lys His Thr Leu Phe Val Leu Ser 145 150 155 160

Be Trp Lys Glu Asn Cys Thr Phe Be Wing Gln Asp Glu Wing Lys Lys

165 170 175

Phe Thr Phe Asn Read Met Lys Wing Gln Ile Met Be Read Asp Pro Gly

180 185 190

Asn Leu Glu Thr Glu Gln Read Lys Lys Glu Tyr Val Cys Phe Met Lys

195 200 205

Gly Val Val Ser Pro Wing Read Asn Leu Pro Gly Thr Wing Tyr Arg Lys

210 215 220

Wing Read Lys Ser Arg Asn Asn Ile Read Lys Phe Ile Glu Gly Lys Met 225 230 235 240

Glu Glu Arg Val Lys Arg Asn Gln Glu Gly Lys Lys Gly Met Glu Glu

245 250 255

Asn Asp Leu Leu Asn Trp Val Leu Lys His Ser Asn Leu Be Thr Glu

260 265 270

Gln Ile Leu Asp Leu Ile Leu Ser Leu Leu Phe Ala Gly His Glu Thr

275 280 285

Be Ser Val Wing Ile Wing Leu Wing Ile Tyr Phe Leu Pro Ser Cys Pro

290 295 300

Gln Ile Wing Gln Gln Read Le Arg Glu Glu His Arg Glu Ile Wing Arg Ser 305 310 315 320

Lys Lys Lys Wing Gly Glu Val Glu Read Thr Thr Asp Asp Tyr Lys Arg

325 330 335

Met Glu Phe His Thr Cys Val Val Asn Glu Thr Leu Arg Leu Gly Asn

340 345 350

Val Val Arg Phe Read His Arg Lys Wing Ile Lys Asp Val His Tyr Lys 355 360 365

Gly Tyr Asp Ile Pro Cys Gly Trp Lys Val Leu Pro Val Ile Ser Ala

370 375 380

Val His Read Asp Pro Ser Asn Phe Asp Gln Pro Gln His Phe Asn Pro 385 390 395 400

Trp Arg Trp Gln Gln Asn Asn Asp

405 410 415

Phe Leu Pro Phe Gly Gly Gly Pro Arg Read Cys Wing Gly Leu Glu Leu

420 425 430

Wing Lys Leu Glu Met Wing Val Phe Ile His His Ile Ile Leu Lys Tyr

435 440 445

Asp Trp Asp Met Val Asp Val Asp Gln Ile Val Tyr Pro Phe Val

450 455 460

Asp Phe Pro Lys Gly Leu Pro Ile Arg Val Gln Ser Gln Wing Thr Leu 465 470 475 480

<210> 89 <211> 1485 <212> DNA

<213> Populus trichocarpa <220>

<221> misc_feature <222> (1110) .. (1110) <223> η is a, c, g, or t <400> 89

atgtctcact cagagcttgt tgtctttctc cttccatcga ttttatcact actcttgctc 60

ttcattctcg tgcaaagaaa gcaagtaaga tttaatctcc caccgggcaa catggggtgg 120 ccatttcttg gagaaaccat tggctacctg aagccttact ctgctacttc aataggagaa 180 ttcatggaac agcacatatc aaggtatgga aagatttaca agtccaattt gtttggggag 240 ccaacaatag tatccgcaga tgctggactt agcagattta tactacagaa tgagggaaga 300 ttatttgaat gcagctatcc aaaaagtatt ggtggaattc ttggaaaatg gtccatgatg 360 gttcttgttg gagacatgca tagagacatg aggattatat ctctcaactt tttgagccat 420 gccaggttaa gaactcatct attgaaagaa gtggagaagc aaaccctgct tgttcttagc 480 tcttggaagg agaattgtac attttcagct caagatgaag caaacaagtt taccttcaat 540 tggatggcaa aacatatcat gagcttggat cctggaaaga cagagactga gcagctgaaa 600 aaagagtatg ttactttcat gaaaggagta gtttcaggtc ctataaattt tcctggaacc 660 ccatatagaa aagccttgaa gtctcgatca atcatcttga aatttataga gcgaaagatg 720 gaggagagaa ttggagaaac gaagggtgga gtagaaaact tggaagacga tgatcttctt 780 ggatgggtct tgaagcattc aaatctttat acggagcaaa tccttgaatt aatcttaagc 840 ttgctctttg ctggccacga aacttcttct gtgtccatag ctctagccat atccttcttg 900 caagcttgtc ctggttcta t tcaacagtta aaagaagaac atattcaaat ctccagagcc 960 aagaaacggt caggagagac ggaattgacc tgggatgatt acaaaaaaat ggaattcact 1020 caatgcgtta taagcgagac agtgaggctt ggaaacgtag tcaggtttgt tcacagaaaa 1080 gctctaaaag atgttcggta caaagggtan gacattccat gtggatggaa agtgttacca 1140 ccgtccattt agattcaact cttttcgacc gtaatctcat aacctcaaca cttcaatcca 1200 tggagatggc agcagcacaa caatgctcgt ggatcttcta cttgttcgag tgcggcggcg 1260 gcggcggcgg cggcggtgag tagtaatcac ttcatgccat ttgggggagg accgcgactc 1320 tgtgcaggat cggaattggc aaaacttgaa atggcagttt tcattcacca tttggttctg 1380 aacttccatt gggaattggt cggtgccgat caagcctttg cctttccttt tgttgatttt 1440 cctaaaggct tgccaataag agtcaagcac cacacagt85 tataa85

<210> <211> <212>

90

494

PRT

<213> Populus trichocarpa <220>

<221> INSECURE <222> (370) .. (370)

<223> Xaa can be any naturally occurring amino acid <400> 90

Met Be His Be Glu Leu Val Val Phe Leu Pro Le I Ser Leu Ser 1 5 10 15

Leu Leu Leu Leu Phe Ile Leu Val Gln Arg Lys Gln Val Arg Phe Asn

20 25 30

Leu Pro Gly Asn Met Gly Trp Pro Phe Leu Pro Gly Glu Thr Ile Gly 35 40 45

Tyr Leu Lys Pro Tyr Be Wing Thr Be Ile Gly Glu Phe Met Glu Gln

50 55 60

His Ile Be Arg Tyr Gly Lys Ile Tyr Lys Ser Asn Leu Phe Gly Glu 65 70 75 80

Pro Thr Ile Val Ser Wing Asp Wing Gly Leu Being Arg Phe Ile Leu Gln

85 90 95

Asn Glu Gly Arg Read Le Phe Glu Cys Ser Tyr Pro Lys Ser Ile Gly Gly

100 105 110

Ile Leu Gly Lys Trp Being Met Met Val Leu Val Gly Asp Met His Arg

115 120 125

Asp Met Arg Ile Ile Be Read Asn Phe Read His Be Wing Arg Read Le Arg

130 135 140

Thr His Leu Leu Lys Glu Val Glu Lys Gln Thr His Leu Leu Val Leu Ser 145 150 155 160

Be Trp Lys Glu Asn Cys Thr Phe Be Wing Gln Asp Glu Wing Asn Lys

165 170 175

Phe Thr Phe Asn Trp Met Wing Lys His Ile Met Being Read Asp Pro Gly

180 185 190

Lys Thr Glu Thr Glu Gln Leu Lys Lys Glu Tyr Val Thr Phe Met Lys

195 200 205

Gly Val Val Ser Gly Pro Ile Asn Phe Pro Gly Thr Pro Tyr Arg Lys

210 215 220

Wing Read Lys Be Arg Be Ile Ile Read Lys Phe Ile Glu Arg Lys Met 225 230 235 240

Glu Glu Arg Ile Gly Glu Thr Lys Gly Gly Val Glu Asn

245 250 255

Asp Asp Leu Leu Gly Trp Val Leu Lys His Ser Asn Leu Tyr Thr Glu

260 265 270

Gln Ile Leu Glu Leu Ile Leu Ser Leu Leu Phe Wing Gly His Glu Thr

275 280 285

Ser Ser Val Ser Ile Wing Leu Wing Ile Ser Phe Leu Gln Wing Cys Pro

290 295 300

Gly Ser Ile Gln Gln Read Lys Glu Glu His Ile Gln Ile Be Arg Wing 305 310 315 320

Lys Lys Arg Be Gly Glu Thr Glu Read Thr Trp Asp Asp Tyr Lys Lys

325 330 335

Met Glu Phe Thr Gln Cys Val Ile Being Glu Thr Val Arg Leu Gly Asn

340 345 350

Val Val Arg Phe Val His Arg Lys Wing Read Lys Asp Val Arg Tyr Lys

355 360 365

Gly Xaa Asp Ile Pro Cys

370 375 380

Val His Read Asp Be Thr Read Le Phe Asp Gln Pro Gln His Phe Asn Pro 385 390 395 400

Trp Arg Trp Gln Gln His Asn Asn Wing Arg Gly Ser Be Thr Cys Ser

405 410 415

Ser Ala Ala Ala Ala Ala Ala Ala Ala Val Ser Ser Asn His Phe Met

420 425 430

Pro Phe Gly Gly Gly Pro Arg Read Cys Wing Gly Be Glu Read Lys Wing

435 440 445

Read Glu Met Wing Val Phe Ile His His Read Leu Val Leu Asn Phe His Trp

450 455 460

Glu Read Val Gly Wing Asp Gln Wing Phe Wing Phe Pro Phe Val Asp Phe 465 470 475 480

Pro Lys Gly Leu Pro Ile Arg Val Lys His His Thr Val Ile 485 490

<210> 91 <211> 1407 <212> DNA

<213> Aquilegia formosa χ Aquilegia pubescens <220>

<221> misc feature <222> (1031) .. (1032) <223> η is a, c, g, or t <400> 91

atgcctgagc ttgtgttttt cttttcatta gctccagcaa ttttagcact aatacttctc 60

ttgaaactct tcaaaaggaa gaaaaagtca tataatcttc caccaggaaa catgggttgg 120

ccatatctag gcgaaactct tggttatttg aagccttatt gtgctatcac tactggagat 180

ttcatggagc aacatatatc aaggtatggg aagatctaca agtcaaattt atttggttat 240

cctacaatag tttcagttga tcctgaatta aatcgatatg tattacaaaa cgaaggaaga 300 ctgtttgaat gtagttatcc aagtagttta ggtgggattc ttggcaaatg gtcaatgtta 360

gttttggttg gagacatgca taaaaacatg aggatgatct ctgtcaactt catgagcagc 420

gcaagacttc gaacacatct aattcaagat gtggagactc aagccttatt ggtgctgaaa 480

tcttggcagg ttgataaaaa gattttggcc caagatgaag caaagaagtt taccttcaat 540

ttaattgtaa aaaatataat gagcatggaa cctggaacac ctgaaagtga gaagcttagg 600

agggaataca ttacattcat gaaaggaatc atttctgcgc ctttgaattt gcctggaact 660

gcatatagaa gagccttaaa gtctcgatcg aacattctgc aacttatcga gcataacatg 720

aatgagagac tccaaaagac taacggagat ggtaagaaag tggaagatga tgatctactt 780

ggatgggtct tgaagcattc aaatcttacc actgaacaaa ttcttgattt gatactaagt 840

atgcttttcg cgggtcatga aacttcctca gtatctatat ctctagccat ataccttttg 900

caaggatgcc taaaagcagt tgaagagtta agggaagagc atattagaat tgctaaagca 960

aaagaacagg caggacagag atctggatta aattgggaag attacaaaca catggaattc 1020

actcaatgtg nntttcttca tagaaaaact ctcaaagatg ttcagtacaa agggtatgac 1080

attccatgtg gttggaaagt tcttccagta tttgcagcag ttcatttgga ccctttaaat 1140

ttcgaccaac ctcatgcttt caatccatgg agatggcaga atgggaagac aagcacgacg 1200

actaacaact tcatgccgtt tggtggtgga ttacggttat gtgctggttc agagctagcc 1260

aagttggaaa tggctatttt cattcaccat ttggtcttga attatgattg ggatatagca 1320

gaaccagatc aaccatttgc ctacccattt gttgaatttc caaaaggtct accaattaag 1380

gtctatgacc atcagtgctt aacatga 1407 <210> 92 <211> 468 <212> PRT

<213> Aquilegia formosa χ Aquilegia pubescens <220>

<221> UNSAFE <222> (344) .. (344)

<223> Xaa can be any naturally occurring amino acid <400> 92

Met Pro Glu Leu Val Phe Phe Phe Ser Leu Wing Pro Wing Ile Leu Wing

1 D IU 13 Leu Ile Leu Leu Leu Lys Leu Phe Lys Arg Lys Lys Ser Tyr Asn 20 25 30 Leu Pro Gly Asn Met Gly Trp Pro Tyr Leu Gly Glu Thr Leu Gly 35 40 45 Tyr Leu Lys Pro Tyr Cys Ala Ile Thr Thr Gly Asp Phe Met Glu Gln 50 55 60 His Ile Be Arg Tyr Gly Lys Ile Tyr Lys Ser Asn Leu Phe Gly Tyr 65 70 75 80 Pro Thr Ile Val Ser Val Asp Asn Glu Gly Arg Leu Phe Glu Cys To Be Tyr Pro To Be Leu Gly Gly 100 105 110 Ile Leu Gly Lys Trp To Met Leu Val Leu Val Gly Asp Met His Lys 115 120 125 Asn Met Arg Met Ile To Be Val Asn Phe Met To Be Wing Arg Leu Arg 130 135 140 Thr His Leu Ile Gln Asp Val Glu Thr Gln Wing Alu Leu Leu Val Leu Lys 145 150 155 160 Ser Trp Gln Val Asp Lys Ile Leu Wing Gln Asp Glu Ala Lys Lys 165 170 175 Phe Thr Phe Asn Leu Ile Val Lys Asn Ile Met Being Met Glu Pro Gly 180 185 190 Pro Thr lu Being Glu Lys Leu Arg Arg Glu Tyr Ile Thr Phe Met Lys 195 200 205 Gly Ile Ile Being Pro Wing Read Asn Leu Pro Gly Thr Wing Tyr Arg 210 210 220 220

Wing Read Lys Be Arg Be Asn Ile Read Gln Read Ile Glu His Asn Met 225 230 235 240

Asn Glu Arg Read Le Gln Lys Thr Asn Gly Asp Gly Lys Lys Val Glu Asp

245 250 255

Asp Asp Leu Leu Gly Trp Val Leu Lys His Ser Asn Leu Thr Thr Glu

260 265 270

Gln Ile Leu Asp Leu Ile Leu Be Met Leu Phe Ala Gly His Glu Thr

275 280 285

Ser Ser Val Ser Ile Ser Leu Wing Ile Tyr Leu Leu Gln Gly Cys Leu

290 295 300

Lys Wing Val Glu Glu Leu Arg Glu Glu His Ile Arg Ile Wing Lys Wing 305 310 315 320

Lys Glu Gln Wing Gly Gln Arg Be Gly Read Asn Trp Glu Asp Tyr Lys

325 330 335

His Met Glu Phe Thr Gln Cys Xaa Phe Read His Arg Lys Thr Read Lys

340 345 350

Asp Val Gln Tyr Lys Gly Tyr Asp Ile Pro Cys Gly Trp Lys Val Leu

355 360 365

Pro Val Phe Asa Wing Val His Leu Asp Pro Leu Asn Phe Asp Gln Pro

370 375 380

His Ala Phe Asn Pro Trp Arg Trp Gln Asn Gly Lys Thr Be Thr Thr 385 390 395 400

Thr Asn Asn Phe Met Pro Phe Gly Gly Gly Read Arg Read Le Cys Wing Gly

405 410 415

Ser Glu Leu Wing Lys Leu Glu Met Wing Ile Phe Ile His His Leu Val

420 425 430

Read Asn Tyr Asp Trp Asp Ile Wing Glu Pro Asp Gln Pro Phe Wing Tyr

435 440 445

Pro Phe Val Glu Phe Pro Lys Gly Leu Pro Ile Lys Val Tyr Asp His

450 455 460

Gln Cys Leu Thr 465

<210> 93 <211> 641 <212> DNA

<213> Triticum aestivum <400> 93

atggccgcca tcatggcctc cataaccagc gagctccttt tcttcctccc cttcatcctc 60

ctggccctgc tcaccttcta caccagcgcc gtggctaaat gccatggcct ccactggtgg 120 agcggccgga cgaagaagag gcggccgaac ctgccgcccg gcgccgtcgg ctggcccttc 180 atcggcgaga ccttcgggta cctccgcgcc cacccggcca cctccatcgg ccagttcatg 240 gaccagcaca tcgcacggta cgggaagata taccggtcga gcctgttcgg ggaccggacg 300 gtggtgtcgg cggacgcggg gctgaaccgg tacatcctgc agaacgaggg gcggctgttc 360 gagtgtagct acccgcggag catcggcggc atccttggca aatggtcgat gctggtgctc 420 gtcggcgacc cccaccgcga gatgcgcttc atctccctca acttcctcag ctccgtccgc 480 ctccgcgccg Tgctcctccc ggaggtggag cgccacaccc tcctcgtcct ccgcgactgg 540 ctgccttact cctcctcctc cgtcttctcc

<210> 94 <211> 213 <212> PRT

<213> Triticum aestivum <400> 94

Met Wing Ile Wing Met Wing Ile Thr Be Glu Leu Leu Phe Phe Leu 15 10 15

Pro Phe Ile Leu Leu Wing Leu Leu Thr Phe Tyr Thr Ser Wing Val Wing

20 25 30

Lys Cys His Gly Read His Trp Trp Being Gly Arg Thr Lys Lys Arg

35 40 45

Pro Asn Leu Pro Pro Gly Wing Val Gly Trp Pro Phe Ile Gly Glu Thr

50 55 60

Phe Gly Tyr Read Arg Wing His Pro Wing Thr Be Ile Gly Gln Phe Met 65 70 75 80

Asp Gln His Ile Arg Wing Tyr Gly Lys Ile Tyr Arg Be Ser Leu Phe

85 90 95

Gly Asp Arg Thr Val Val Ser Wing Asp Wing Gly Leu Asn Arg Tyr Ile

100 105 110

Read Gln Asn Glu Gly Arg Read Phe Glu Cys Be Tyr Pro Arg Be Ile

115 120 125

Gly Gly Ile Leu Gly Lys Trp Ser Met Leu Val Leu Val Gly Asp Pro

130 135 140

His Arg Glu Met Arg Phe Ile Be Read Asn Phe Read Be Be Val Arg 145 150 155 160

Leu Arg Val Wing Leu Leu Pro Glu Val Glu Arg His Thr Leu Leu Val

165 170 175

Leu Arg Asp Trp Leu Pro Tyr Ser Ser Ser Ser Val Phe Ser Ala Gln

180 185 190

His Glu Alys Lys Lys Phe Thr Phe Asn Leu Met Alys Lys Asn Ile Met

195 200 205

Ser Met Asp Pro Gly 210 <210> 95 <211> 791 <212> DNA

<213> Triticum aestivum <400> 95

ggaaggccct caagtcgcga gccaccatac ttggagtaat ggcttgagaa aatgaataag gaggcctcga gcatggaaga cgatgaagca gtccaatctt tcgaaagaac aaatattgga ttgccgggca tgagacatcg tcaatggcgc tcgccctcgc gccctaaagc cgttgaagaa ctgcgggagg agcatcttga tgagagggga gtgcaaactg agctgggaag actacaaaga ttataaacga gaccttgcgg ctaggcaacg tggtcaggtt gagatgtgca ctacaatggg tatgacatcc cgagcggatg cggcggtgca tctcgactcg tcgctgtacg aggaccccag ggaagggcaa cgcgtccggc gtggcgcaga acagtaactt cgaggctctg cgccgggtcg gagctcgcca agctcgagat tggtgctcaa cttccggtgg gagctcgccg agcccgacca tcgacttccc caagggcctg cccatcaggg tccataggat aagaggagta at <210> 96 <211> 261 <212> PRT

<213> Triticum aestivum <400> 96

Lys Wing Leu Lys Be Arg Wing Thr Ile Leu Gly Val Ile Glu Arg Lys 15 10 15

Met Glu Glu Arg Read Leu Glu Lys Met Asn Lys Glu Wing Ser Be Met Glu

20 25 30

Glu Asp Asp Leu Read Gly Trp Wing Met Lys Gln Be Asn Leu Be Lys

35 40 45

Glu Gln Ile Leu Asp Leu Leu Leu Seru Leu Phe Ala Gly His Glu

50 55 60

Thr Be Ser Met Wing Leu Wing Leu Wing Ile Phe Phe Leu Glu Gly Cys 65 70 75 80

Pro Lys Wing Valu Glu Glu Leu Arg Glu Glu His Leu Glu Ile Arg Wing

85 90 95

Arg Gln Lys Read Arg Gly Glu Cys Lys Read Ser Trp Glu Asp Tyr Lys

100 105 110

Glu Met Val Phe Thr Gln Cys Val Ile Asn Glu Thr Leu Arg Leu Gly

115 120 125

Asn Val Val Arg Phe Read His Arg Lys Val Ile Arg Asp Val His Tyr

130 135 140

Asn Gly Tyr Asp Ile Pro Ser Gly Trp Lys Ile Leu Pro Val Leu Wing 145 150 155 160

agagaggaaa agatgatctt cctcttgctg catcttcttc gattgctagg gatggttttc cctgcaccgg gaaaattctg cagcttcaac catgccctac ggccatcttc ggcgttcgtg tgcacaggaa

atggaggaaa ctcgggtggg agcttgctgt ctcgaaggtt agacagaagc acgcaatgcg aaggtcattc ccggtgttag ccttggagat ggcggcggca ctgcaccacc tacccgttcg gaagaaggag

60 120 180 240 300 360 420 480 540 600 660 720 780 791 Wing Val His Read Asp Ser Be Read Tyr Glu Asp Pro Be Phe Asn

165 170 175

Pro Trp Arg Trp Lys Gly Asn Wing Be Gly Val Wing Gln Asn Be Asn

180 185 190

Phe Met Pro Tyr Gly Gly Gly Thr Arg Leu Cys Wing Gly Ser Glu Leu

195 200 205

Wing Lys Leu Glu Met Wing Ile Phe His His Leu Val Leu Asn Phe

210 215 220

Arg Trp Glu Leu Glu Pro Wing Asp Gln Wing Phe Val Tyr Pro Phe Val 225 230 235 240

Asp Phe Pro Lys Gly Leu Pro Ile Arg Val His Arg Ile Wing Gln Glu

245 250 255

Glu Glu Gly Glu Glu 260

<210> 97 <211> 789 <212> DNA

<213> Euphorbia esula <220>

<221> misc_feature <222> (325) .. (326) <223> η is a, c, g, or t <400> 97

agcatggacc ctggaaaacc agagactgaa aagctcaaga aagaatatgt tactttcatg aaaggagttg tttctgctcc tattaatttg cctggaactg cttatagaag ggccttacag tctcgatcga caattttgaa gttcatagag gagaaaatgg aggaaagaaa tgaaaaatta aaagaaggaa aagcagagga agaagaagaa gatgatcttc taggatgggt tttaaagcat tcaaatcttt caactgagca aattctagat ttggtattaa gtttaatgtt tgctggccat gaaacttctt cagtagcaat tcttnnagct atctattttt tgcaagattc tcctgctgct cttcaacagc taagggaaga acataaggaa attgaaaaag ccaagaagca gtcaggagag aagggattga actgggatgt ttacaaaaat atggaattca ctcagtgtgt tatcaatgaa acactaagac ttggaaatgt agtcaggttc cttcatagga agactattaa acacgttcaa tacaaaggat atgacattcc acgtggatgg aaagtgctgc cagtgattgc agccgtgcat ttggacagta gccattttga gaagcctcaa cacttcaatc catggagatg gttgcaccag aataatggga tacaaaatat gaataataat ttcatgccat ttgggggagg accaagatta tgtgcaggat cagaattagc aaaactagaa atggctattt ttattcatca tttggtcctt aattaccag <210> 98 <211> 263 <212> PRT

<213> Euphorbia esula <220>

<221> UNSAFE <222> (109) .. (109)

<223> Xaa can be any naturally occurring amino acid <400> 98

Ser Met Asp Pro Gly Lys Pro Glu Thr Glu Lys Leu Lys Lys Glu Tyr 1 5 10 15 Val Thr Phe Met 20 Lys Gly Val Val Ser 25 Wing Pro Ile Asn Leu 30 Pro Gly Thr Wing Tyr Arg Wing Thr Ile Read Lys Phe

35

Ile Glu Glu Lys 50

40

45

Met Glu Glu Arg Asn Glu Lys

55

60

Glu Wing Glu Glu Glu Glu Asp Asp Leu Leu Gly Trp Val Leu Lys His

65

70

75

80

Ser Asn Leu Ser Thr Glu Gln Ile Leu Asp Leu Val Leu Ser Leu Met

85 90 95

Phe Ala Gly His Glu Thr Be Ser Val Ala Ile Leu Xaa Ala Ile Tyr

100 105 110

Phe Leu Gln Asp Ser Pro Wing Ward Leu Gln Gln Leu Arg Glu Glu His

115 120 125

Lys Glu Ile Glu Lys Wing Lys Lys Gln Ser Gly Glu Lys Gly Leu Asn

60 120 180 240 300 360 420 480 540 600 660 720 780 789 130 135 140

Trp Asp Val Tyr Lys Asn Met Glu Phe Thr Gln Cys Val Ile Asn Glu 145 150 155 160

Thr Read Le Arg Read Gly Asn Val Val Arg Phe Read His Arg Lys Thr Ile

165 170 175

Lys His Val Gln Tyr Lys Gly As Tyr Asp Ile Pro Arg Gly Trp Lys Val

180 185 190

Leu Pro Val Ile Wing Val Val Wing Leu Asp Ser Be His Phe Glu Lys

195 200 205

Pro Gln His Phe Asn Pro Trp Arg Trp Read His Gln Asn Asn Gly Ile

210 215 220

Gln Asn Met Asn Asn Asn Pn Met Asn Pn Gn Gly Gly Gly Pro Arg Leu 225 230 235 240

Cys Wing Gly Ser Glu Leu Wing Lys Leu Glu Met Wing Ile Phe Ile His

245 250 255

His Leu Val Leu Asn Tyr Gln 260

<210> 99 <211> 955 <212> DNA

<213> Gossypium hirsutum <400> 99

atgcctgact cagagcctat tttcttgctt cttccatcta ttttatcttt gattctgttc ttcattctca tcaagagaaa gcaaagaagg tataatcttc caccagggaa catggggtgg ccttttctcg gcgaaacaat cggttacttg aggccttact ctgctacttc agtaggtgaa ttcatgcacc agcatatatc aaggtatggg aatatctaca aatcgaattt gtttggtgag aagacaatag tgtctgcaga tgctgggttg aacaagttca tattacaaaa cgaagggaga ttattcgagt gcagttaccc gagaagcatt ggtggcattc ttgggaaatg gtcgatgctg gttttggttg gggatatgca tagagacatg aggattatat cactcaactt cttgagcaac gccaggctga ggactcatct tttgagagaa gtggagaaac atactttgct tgttctaaat acttggaaag agaagtgcat attttcagct caggatgaag caaaaaagtt cactttcaat ttgatggcaa aaaatatcat gagcatggac cctggacatc cagagacgga gcagctaaag aaagaatatg ttactttcat gaaaggagtc gtttctgctc ctttaaattt acctggaact gcatacagaa aagccttaca atctcgatca acaatcctga aatttattga aaagaaaatg gaagtaagga taaggaaaat gaaggaagga aaagaaaact cagaggaaga tgatcttctt gaatgggtct taaagcattc taatctttcc acagagcaaa tccttgactt gattttgagc ttgctttttg ctggacatga gacttcctcg gtagccataa ccttagccat ctacttcttg ccaggttgtc ctttggccat tcaacagttg agagaggaac accttgaagt tgcag <210> 100 <211> 333 <212> PRT

<213> Gossypium hirsutum <220>

<221> UNSAFE <222> (326) .. (326)

<223> Xaa can be any naturally occurring amino acid <400> 100

Met Pro Asp Be Glu Pro Ile Phe Leu Leu Leu Pro Ser Ile Leu Ser 15 10 15

Leu Ile Leu Phe Phe Ile Leu Ile Lys Arg Lys Gln Arg Arg Tyr Asn

20

25

30

Read Pro Gly Asn Met Gly Trp Pro Phe Read Gly Glu Thr Ile Gly

35

40

45

Tyr Leu Arg Pro Tyr Be Wing Thr Be Val Gly Glu Phe Met His Gln

50

55

60

His 65

Ile Be Arg Tyr Gly Asn Ile Tyr Lys Be Asn Leu Phe Gly Glu

70

75

80

Lys Thr Ile Val Ser Wing Asp Wing Gly Leu Asn Lys Phe Ile Leu Gln

85

90

95

Asn Glu Gly Arg Read Phe Glu Cys Be Tyr Pro Arg Be Ile Gly Gly

100

105

110

Ile Leu Gly Lys Trp Being Met Leu Val Leu Val Gly Asp Met His Arg

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 955

115

120

125 Asp Met Arg Ile Ile Ser Read Asn Phe Read Ser

130

135

Thr His Leu Leu Arg Glu Val Glu Lys His Thr

Asn Wing 140

Leu Leu

Arg Leu Val Leu

Arg

Asn

145

150

165

170

180

185

195

200

210

215

225

230

245

250

260

265

275

280

290

295

305

310

155 160 Gln Asp Glu Wing Lys 175 Lys Met Ser As As 190 Pro Gly Tyr Val Thr 205 Phe Met Lys Gly Thr 220 As Tyr Arg Lys Phe Ile Glu As Lys Met 235 240 Lys Glu Asn As Glu 255 Thr Glu Phe Ala Gly 285 His Glu Thr Phe Leu 300 Pro Gly Cys Pro Leu Lys Leu Gln Ser Gln 315 320 Asp Thr Glu

325

330

<210> 101 <211> 832 <212> DNA

<213> Lycopersicon esculentum <4 00> 101

atgggttggc cttttcttgg tgaaactatt ggctatttga gaccttattc agctactact 60

attggagatt tcatgcaaga tcatatctct aggtatggga aaattttcaa gtcaaatttg 120

tttggagagc caacaatagt ttcagcagat gcagggctaa acagatacat tctgcagaat 180

gaagggagat tatttgagtg taattatcca agaagtatag gtgggatact tggtaaatgg 240 tctatgttag ttcaagttgg acaaatgcat agagatatga ggatgatttc tctgaatttt 300

ttgagcaatg ctaggctaag gaatcaactt ttaagtgaag ttgaaaagca tactttgctt 360

gttcttggct cttggaaaca ggattctgtt gtttgtgcac aagatgaagc aaagaagtta 420

acattcaact ttatggcaga gcatatcatg agtctacaac ctggaaatcc agagacagag 480

aagctgaaaa aagagtacat cacatttatg aaaggagtgg tttctgctcc attgaatttt 540

ccaggaacag cttacagaaa ggccttacag tctcgatcaa caattcttgg atttattgag 600

agaaaaatgg aggagaggct taaggaaatg aacagaaacg aaaacgacct tctaggttgg 660

gttctgaaga attcaaatct ctcaaaagag caaattcttg atttgctact gagtttgctc 720

tttgctggcc atgaaacttc atcagtagca atagctctgt ctattttctt actcgaaagc 780

tgtcctgctg ctgttcaaca attaacagaa gagcacttgg agatttcccg gg 832 <210> 102 <211> 277 <212> PRT

<213> Lycopersicon esculentum <4 00> 102

Met Gly Trp Pro Phe Leu Gly Glu Thr Ile Gly

10

Tyr Leu Arg

Be Wing Thr Thr Ile Gly Asp Phe Met Gln Asp His

20 25

Gly Lys Ile Phe Lys Ser Asn Leu Phe Gly Glu Pro

35

40

Wing Asp Wing Gly Leu Asn Arg Tyr Ile Leu Gln

50

55

Phe Glu Cys Asn Tyr Pro Arg Be Ile Gly Gly

Asn 60 Ile

Ile Ser

30 Thr Ile 45

Glu Gly

65

70

75

Ser Met Leu Val Gln Val Gly Gln Met His Arg Asp

85 90

Ser As Leu Asn Phe Ser As Le Asn Ala Arg As Le Arg Asn

Read Gly Met Arg Gln Read

Pro Tyr 15

Arg tyr

Val ser

Arg Leu

Lys Trp 80 Met Ile 95

Read Ser 100 105 110

Glu Val Glu Lys His Thr Leu Leu Val Leu Gly Ser Trp Lys

115 120 125

Ser Val Val Cys Wing Gln Asp Glu Wing Lys Lys Leu Thr Phe

130 135 140

Met Wing Glu His Ile Met Being Read Gln Pro Gly Asn Pro Glu 145 150 155

Lys Leu Lys Lys Glu Tyr Ile Thr Phe Met Lys Gly Val Val

165 170

Pro Leu Asn Phe Pro Gly Thr Wing Tyr Arg Lys Wing Leu Gln 180 185 190

Being Thr Ile Read Gly Phe Ile Glu Arg Lys Met Glu Glu Arg

195 200 205

Glu Met Asn Arg Asn Glu Asn Asp Leu Leu Gly Trp Val Leu

210 215 220

Ser Asn Leu Ser Lys Glu Gln Ile Leu Asp Leu Leu Le Ser 225 225 235

Phe Ala Gly His Glu Thr Be Ser Val Val Ile Ala Leu Ser

245 250

Leu Leu Glu Ser Cys Pro Wing Val Wing Gln Gln Leu Thr Glu 260 265 270

Read Glu Ile Ser Arg

275 <210> 103 <211> 711 <212> DNA

<213> Solanum tuberosum <400> 103

atgtctgact tagagttttt tctttttctt gttcctccaa tcttggcagt cttaatctat tcaaaagaaa acacaaattt caaaatcttc caccagggga ccttttcttg gtgaaactat tggttatttg agaccttact cagttactac ttcatgcaag atcatatctc aaggtatggg aaaattttca agtcaaattt ccaacaattg tttcagcaga tgcagggctt aacagataca ttctgcagaa ttatttgagt gtaattatcc aagaagtata ggtgggatac ttggtaaatg gttcaagttg gacaaatgca tagagatatg aggatgattt ctctgaattt gctagactca ggaatcaact tttaagtgaa gttgaaaagc atactgtgct tcttggaaac aggattctgt tgtttgtgca caagatgaag caaagaagtt tttatggcag agcatatcat gagtctacaa cctggaaatc cagagacaga aaagagtaca tcacatttat gaaaggagtg gtttctgctc cattgaattt gcttacagaa aagccctaca gtctcgatca acaattcttg gaattattga <210> 104 <211> 237 <212> PRT

<213> Solanum tuberosum <400> 104

Met Ser Asp Leu Glu Phe Phe Leu Phe Leu Val 1 5 10

Val Leu Ile Ile Leu Asn Leu Phe Lys Arg Lys

20 25

Read Pro Gly Asp Met Gly Trp Pro Phe Leu

35 40

Tyr Leu Arg Pro Tyr Be Val Thr Thr Ile Gly Asp

50 55 60

His Ile Ser Arg Tyr Gly Lys Ile Phe Lys Ser Asn Leu Phe 65 70 75

Pro Thr Ile Val Ser Wing Asp Wing Gly Leu Asn Arg Tyr Ile

85 90

Asn Glu Gly Arg Read Phe Glu Cys Asn Tyr Pro Arg Ser Ile 100 105 110

Ile Leu Gly Lys Trp Being Met Leu Val Gln Val Gly Gln Met

115 120 125

Asp Met Arg Met Ile Be Read Asn Phe Read Be Asn Wing Arg 130 135 140

Gln

Asn

Thr

Being 175 Being

Read

Lys

Read

Ile 255 Glu

Asp

Phe

Glu 160 Wing

Arg

Lys

Asn

Read 240 Phe

His

ccttattatt tatgggttgg tattggagat gtttggagag tgaagggaga gtctatgttg tttgagcaat tgttcttggc tacattcaac gaagctgaag tccaggaaca

g

Pro Pro Ile

His Lys Phe 30

Gly Glu Thr 45

Phe Met

Read Wing 15

Gln asn

Ile gly

Gln asp

Gly Glu 80

Read Gln 95

Gly Gly His Arg Read Arg

60 120 180 240 300 360 420 480 540 600 660 711 Asn Gln Leu Leu Be Glu Val Glu Lys His Thr Val Leu Val Leu Gly 145 150 155 160

Be Trp Lys Gln Asp Be Val Val Cys Wing Gln Asp Glu Wing Lys Lys

165 170 175

Phe Thr Phe Asn Phe Met Glu Wing His Ile Met Being Read Gln Pro Gly

180 185 190

Asn Pro Glu Thr Glu Lys Read Lys Lys Lys Glu Tyr Ile Thr Phe Met Lys

195 200 205

Gly Val Val Ser Wing Pro Read Asn Phe Pro Gly Thr Wing Tyr Arg Lys

210 215 220

Wing Read Gln Be Arg Be Thr Ile Read Gly Ile Ile Glu 225 230 235

<210> 105 <211> 431 <212> DNA

<213> Solanum tuberosum <4 00> 105

ggaaagctgt gaaagatgtt cgatacaaag gttatgacat tccatgtgga tggaaagtat 60

tgccggtgat ttcagcagcg catttagatc cttcactttt tgaccgacct cacgactttg 120 atccttggag atggcagaat gcagaagagt cgccttcagg taaaggagga agcacaggca 180 caagcagcac aacaaaaagt agtaataatt tcatgccatt tgggggaggt ccacgtctat 240 gtgcaggatc tgaactggcc aaacttgaga tggccatttt cattcactat cttgttctta 300 attttcactg gaaattagct gcacctgatc aggcttttgc ctatccttac gtagattttc 360 ccaatgccct accaatcact atccaacatc gatcgtcaaa taaattaaac caccacactt 420 to 431 actacctcta

<210> 106 <211> 142 <212> PRT

<213> Solanum tuberosum <4 00> 106

Lys Wing Val Lys Asp Val Arg Tyr Lys Gly Tyr Asp Ile

Trp Lys Val Leu Pro Val Ile Ser Ward Wing His Leu Asp Pro Ser Leu

20 25 30

Phe Asp Arg Pro His Asp Phe Asp Pro Trp Arg Trp Gln Asn Wing Glu

35 40 45

Glu Be Pro Be Gly Lys Gly Gly Be Thr

50 55 60

Lys Being Ser Asn Asn Phe Met Pro Phe Gly Gly Gly Pro Arg Read Le Cys 65 70 75 80

Wing Gly Ser Glu Leu Wing Lys Leu Glu Met Wing Ile Phe Ile His Tyr

85 90 95

Leu Val Leu Asn Phe His Trp Lys Leu Wing Pro Wing Asp Gln Wing Phe

100 105 110

Tyr Wing Pro Tyr Val Asp Phe Pro Asn Wing Read Pro Ile Thr Ile Gln

115 120 125

His Arg Being Ser Asn Lys Leu Asn His His Thr Tyr Tyr Leu

130 135 140

<210> 107 <211> 52 <212> DNA

<213> Artificial sequence <220>

<223> initiator: prm06540 <4 00> 107

ggggacaagt ttgtacaaaa aagcaggctt aaacaatggc cgccatgatg gc 52

<210> 108 <211> 48 <212> DNA

<213> Artificial sequence <220>

<223> initiator: prm06520 <4 00> 108 ggggaccact ttgtacaaga aagctgggtt

<210> 109

<211> 654

<212> DNA

<213> Oryza sativa

<400> 109

cttctacatc ggcttaggtg tagcaacacg ttattttaca aaaatataaa atagatcagt ttattgtaaa gttctacaaa gctaatttaa aaacaagagt gtcaatggaa caatgaaaac attgaaatta tataattcaa agagaataaa gcattaccaa aatatatata gcttacaaaa ccttatcaca ttgacacata aagtgagtga atcatgtata tatgatagcc acaaagttac gtgcacctaa cagaatatcc aaataatatg aaaactaaca ctctaaagca accgatggga aatcctcatc atccttcacc acaattcaaa <210> 110 <211> 1236 <212> DNA <213> Oryza sativa <400> 110

ggtcagccaa tacattgatc cgttgccaat ccggaattga taattgtgtt ctgactaaat tatccgaaac ctggattaaa caatcctgtt ttgttttttt gacatgtttt cttgactgag ttttaacttt ttcatcacat cagaactttt acatcgttcc aatttcaatc aaactttcaa ttttattgct cctccgtacg ggttggctgg tagatattgg gaggaacttg gcatgaatgg tgaggaggta ccatgaggta ccaaaatttt ggtattatga ggtaccaaat ttacaataga taccgtaaaa ttgctcctat atttaagggg attttgataa gaaaaaatgt gagcacacca actcgtcaac tgtgaagaac ctcaaaaatg aactttaaag ttttgaacat cgatttcact tgatagttta gaactctaga atcattgtcg tccagctatg aaaaaaccct caccaaacac gcggccatgc tgtcacgcaa cgcaccgcat tccccgtgca catatccgac agacgcgccg gcccatgcaa gcatccaccc ccgccacgta tctctccacc tatatatgcc cacctggccc cttcttcttc ttcttcgttg cattcatctt <210> 111 <211> <p> <p>

<221> misc_feature <222> (1043) .. (1044) <223> is not a, c, g, or t <400> 111

atgtctgact cactcttaac tttctattct ttcatcttca ttctcatcaa aagaaagcaa aacatgggtt ggccatttct tggtgaaacc acagtagggg aattcatgga gcaacacata ctgtttgcgg ggccagcaat agtgtcagca aacgaaggga aattgttcga gtgcagctat tggtccatgt tggtcttagt tggtgacatg tttctaagcc acgccaggct cagaacacac ttggttctga actcttggag ccaaaattcc ttcaccttca atttaatggc taagcatatc gagcaactaa agaaagagta cgtcactttc

tactcctgct catcatcc

48

actttattat ccctcaccac aagttattgc catatgacat tccacatagc catgacaagc tgagtcataa tttgatgatg actcacttag aagcatctat tattatagtt

tattattatt aagtagagca attaacttat actataattt cgtaaagttc ttagtttgaa tattattttc atatcaaaga atcataatag aaatagacaa gaagcatagt

attattatta agttggtgag ttcatattac tgtttttatt tacatgtggt aaattgcaat tttgctaccc acatttttag agcatcaagt gcacaatgaa agta

catgcaaagt taaatgacca ctgttctcta gcctgtttgt ctacacatat ttttggcgtg ttgagaatag ccactatatt agtgtaaatt aaaaatagta atgtttatat agcatgtcca ctcaatatag aaacaacaat gcggagaaggcccctagtctcta

attttggctg gaagtcgcta gcccctcctg tctaaacttt aaacttttaa aactaaacac gtattttcag tagagcaatt ttagtatctc cttcatggta ctatccatat tgaccttgca ctacaggtgc tattatcccccctctctcgctg

tggccgagtg tcttccaatg catggccgga ttcttcaaac cttttccgtc accctgagtc agagaaaatc ctacggtcct attataacta ctttcttaag ccataatttg ctcttggctc ctgaaaaaat ctctgggacctcgggtcgctg

ctttcagcca agcaaaccca attggctatt gcaaggtatg gatgcaggac cctagaagca catagagaca ctcttgaaag atattctcag atgagcatgg atgaaagggg

ttcttgctct ggctcaacct tgaagcctta gtacaattta tcaacaggtt tcggtggaat tgcgggttat aggtggagaa cccaagatga atcctgggga tggtttccgc

tctcccaatc tcccccaggt ttctgccacc caagtcaaaa cattctacaa actaggaaaa atcactcaac gcaatccctc agctaagaag tatcgagaca gccattgaat

60 120 180 240 300 360 420 480 540 600 654

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1236

60 120 180 240 300 360 420 480 540 600 660 ttacctggaa ctgcataccg aaaggcattg aagtctcggt ccattatact aaagttcata gaggggaaaa tggaagagag agttagaaga atccaagagg ggaatgagag tttggaggaa gatgatcttc taaattgggt tttgaagaat tcaaatcttt caaccgagca aattcttgac ttgattctca gcttgctctt cgctggccat gaaacttcgt cggtagccat agctctagcc atttacttct tacccggttg tcctcaagct atacaacagt taaaggaaga acacagagaa attgccagag ccaaaaagca agcaggggaa gttgaactca cttgggatga ctacaaacga atggaattta ctcattgtgt aannttggga aatgttgtga ggtttctcca caggaaggct gtgaaagatg ttaactataa aggttatgac attccatgtg ggtggaaagt cctcccggtg attgcagccg tgcatctgga tccttcactt tttgaccaac ctcaacactt caatccatgg agatggcaga acaatggcag tcatggaagt tgtccaagca agaacacagc aaacaacaac tttcttccgt tcggaggagg accacgatta tgtgcaggat cagagttagc taagcttgaa atggctgttt tcattcacca tctcattctc aactaccatt gggaattggc tgataccgat caagcttttg cctacccttt tgtcgacttc cccaaaggcc tacccgttag agtccaagcc cattctttac tttga <210> 112 <211> 530 < 212> PRT <213> Glycine max <220>

<221> UNSAFE <222> (391) .. (392)

<223> Xaa can be any naturally occurring amino acid <4 00> 112

Met Ser Asp Ser Leu Leu Thr Phe Tyr Ser Leu Sera Wing Ile Leu Wing 15 10 15

Read Leu Pro Ile Phe Ile Phe Ile Read Ile Lys Arg Lys Gln Ser Lys

20 25 30

Pro Arg Read Asn Leu Pro Pro Gly Asn Met Gly Trp Pro Phe Leu Gly

35 40 45

Glu Thr Ile Gly Tyr Read Lys Pro Tyr Ser Wing Thr Thr Val Gly Glu

50 55 60

Phe Met Glu Gln His Ile Wing Arg Tyr Gly Thr Ile Tyr Lys Ser Lys 65 70 75 80

Leu Phe Wing Gly Pro Wing Ile Val Ser Wing Asp Wing Gly Leu Asn Arg

85 90 95

Phe Ile Read Gln Asn Glu Gly Lys Read Phe Glu Cys Ser Tyr Pro Arg

100 105 110

Ser Ile Gly Gly Ile Leu Gly Lys Trp Ser Met Leu Val Leu Val Gly

115 120 125

Asp Met His Arg Asp Met Arg Val Ile Be Read Asn Phe Read His Be

130 135 140

Arg Wing Arg Read Arg Thr His Leu Read Lys Glu Val Glu Lys Gln Ser Leu 145 150 155 160

Leu Val Leu Asn Ser Trp Ser Gln Asn Ser Ile Phe Ser Wing Gln Asp

165 170 175

Glu Alys Lys Lys Phe Thr Phe Asn Leu Met Alys Lys His Ile Met Ser

180 185 190

Met Asp Pro Gly Asp Ile Glu Thr Glu Gln Read Lys Lys Lys Glu Tyr Val

195 200 205

Thr Phe Met Lys Gly Val Val Ser Wing Pro Leu Asn Leu Pro Gly Thr

210 215 220

Wing Tyr Arg Lys Wing Read Lys Be Arg Be Ile Ile Read Lys Phe Ile 225 230 235 240

Glu Gly Lys Met Glu Glu Arg Val Arg Arg Ile Gln

245 250 255

Be Leu Glu Glu Asp Asp Leu Leu Asn Trp Val Leu Lys Asn Ser Asn

260 265 270

Leu Ser Thr Glu Gln Ile Leu Asp Leu Ile Leu Ser Leu Leu Phe Ala

275 280 285

Gly His Glu Thr Be Ser Val Wing Ile Wing Leu Wing Ile Tyr Phe Leu

290 295 300

Pro Gly Cys Pro Gln Wing Ile Gln Gln Read Lys Glu Glu His Arg Glu 305 310 315 320

720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1455 Ile Arg Wing Lys 325 Wing Lys Gln Wing Gly Glu 330 Val Glu Leu Thr Trp 335 Asp Tyr Lys Arg 340 Met Glu Phe Thr His 345 Cys Val Ser Gly Met 350 Ile Ile Asn Glu Leu 355 Trp Arg Be Thr Cys 360 Leu Phe Phe Tyr Phe 365 Met Tyr Ser Asn Cys 370 Tyr Tyr Tyr Tyr Leu 375 Met Asn Met Leu Val 380 Val As Asn Ile Ile Trp Asp Asp Glu Leu Gly Asn Val Val Arg 385 390 395 400 Phe Leu His Arg Lys 405 Wing Val Lys Asp Val 410 Asn Tyr Lys Gly Tyr 415 Asp Ile Pro Cys Gly 420 Trp Lys Val Leu Pro 425 Val Ile Wing Val 430 His Leu Asp Pro Ser 435 Leu Phe Asp Pro Gln 440 Gln His Phe Asn Pro 445 Trp Arg Trp Gln Asn 450 Asn Gly Being His Gly 455 Ser Cys Pro Being Lys 460 Asn Thr Aln Asn Phe Leu Pro Phe Gly Gly Gly Pro Gly Ser 465 470 475 480 Glu Leu Wing Lys Leu 485 Glu Met Wing Val Phe 490 Ile His His Leu Ile 495 Leu Asn Tyr His Trp 500 Glu Leu Wing Asp Thr 505 Asp Gln Ala Phe Ala 510 Tyr Phe Pro Val Asp 515 Phe Pro Lys Gly Leu 520 Pro Val Arg Val Gln 525 His His Wing

Leu Leu 530 <210> 113 <211> <212>

1458 DNA

<213> Gossypium hirsutum <400> 113

atgcctgact cagagcctat tttcttgctt ctaccatcta ttcattctca tcaagagaaa gcaaagaagg tataatcttc ccttttctcg gcgaaacaat cggttacttg aggccttact ttcatgcacc agcatatatc aaggtatggg aatatctaca aagacaatag tgtctgcaga tgctgggttg aacaagttca ttattcgagt gcagttaccc gagaagcatt ggtggcattc gttttggttg gggatatgca tagagacatg aggattatat gccaggctga ggactcatct tttgagagaa gtggagaaac acttggaaag agaagtgcat attttcagct caggatgaag ttggtggcaa aaaatatcat gagcatggac cctggacatc aaagaatatg ttactttcat gaaaggagtc gtttctgctc gcatacagaa aagccttaca atctcgatca acaatcctga gaagtaagga taaggaaaat gaaggaagga aaagaaaact gaatgggtct taaagcattc taatctttcc acagagcaaa ttgctttttg ctggacatga gacctcctcg gtagccataa ccaggttgtc ctttggccat tcaacagttg agagaagaac aagaaccaat caggagagac tgaactcaac tgggatgatt caatgtgtta ttaacgagac acttaggctt ggtaatgtcg gctctcaaag atattagata taaaggttat gatattccat gtgattgcag cagtgcactt ggatccctgt ctttttgacc tggcgatggc agcaaaataa tgggagtcga ggggcgggga agcagcaatt acttcatgcc attcggggga ggaccacggc gctaaactgg aaatggcggt gttcatccac catttggtcc gccgatacgg atgaagcctt tgccttccct tttgtcgact agagtcttca aatcttaa <210> 114 <211> 485 <212> PRT

<213> Gossypium hirsutum <400> 114

ttttatcttt caccagggaa ctgctacttc aatcgaattt tattacaaaa ttgggaaatg cactcaactt atactttgct caaaaaagtt cagagacgga ctttaaattt aatttattga cagaggaaga tccttgactt ccttagccat accttgaagt acaagaaaat tcagatttct gtgggtggaa accctcaact cggcaacgtc tatgtgcagg tcaactacca tccctaaagg

gattctgttc catggggtgg agtaggtgaa gtttggtgag cgaagggaga gtcgatgctg cttgagcaac tgttctaaat cactttcaat gcagctaaag acctggaact aaagaaaatg tgatcttctt gattttgagc ctacttcttg tgccagagcc ggagttcact ccacagaaaa agtgcttcca cttcaatcca atcagcgagc aacagagctg gtgggagtta cctacccatc

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1458 Met Pro Asp Ser Glu 1 5

Leu Ile Leu Phe Phe 20

Read Pro Pro Gly Asn 35

Tyr Leu Arg Pro Tyr 50

His Ile Ser Arg Tyr 65

Lys Thr Ile Val Ser 85

Asn Glu Gly Arg Leu 100

Ile Leu Gly Lys Trp 115

Asp Met Arg Ile Ile 130

Thr His Leu Leu Arg 145

Thr Trp Lys Glu Lys 165

Phe Thr Phe Asn Leu 180

His Pro Glu Thr Glu 195

Gly Val Val Ser Wing 210

Wing Read Gln Ser Arg 225

Glu Val Arg Ile Arg 245

Asp Asp Leu Leu Glu 260

Gln Ile Leu Asp Leu 275

Ser Ser Val Wing Ile 290

Read Ala Ile Gln Gln 305

Lys Asn Gln Ser Gly 325

Met Glu Phe Thr Gln 340

Val Val Arg Phe Leu 355

Gly Tyr Asp Ile Pro 370

Val His Leu Asp Pro 385

Trp Arg Trp Gln Gln 405

Ser Ser Ala Ser Ser 420

Arg Read Cys Wing Gly 435

Ile His His Leu Val 450

Glu Ala Phe Ala Phe 465

Arg Val Phe Lys Ser 485

<210> 115

For Ile Phe Leu Leu 10

Ile Leu Ile Lys Arg 25

Met Gly Trp Pro Phe 40

Ser Wing Thr Ser Val 55

Gly Asn Ile Tyr Lys 70

Wing Asp Wing Gly Leu 90

Phe Glu Cys Ser Tyr 105

Ser Met Leu Val Leu 120

Ser Leu Asn Phe Leu 135

Glu Val Glu Lys His 150

Cys Ile Phe Ser Ala 170

Val Ala Lys Asn Ile 185

Gln Leu Lys Lys Glu 200

Pro Leu Asn Leu Pro 215

Ser Thr Ile Leu Lys 230

Lys Met Lys Glu Gly 250

Trp Val Leu Lys His 265

Ile Leu Ser Leu Leu 280

Thr Leu Wing Ile Tyr 295

Read Arg Glu Glu His 310

Glu Thr Glu Leu Asn 330

Cys Val Ile Asn Glu 345

His Arg Lys Wing Leu 360

Cys Gly Trp Lys Val 375

Cys Leu Phe Asp His 390

Asn Asn Gly Ser Arg 410

Ser Asn Tyr Phe Met 425

Thr Glu Leu Wing Lys 440

Read Asn Tyr Gln Trp 455

Pro Phe Val Asp Phe 470

Leu Pro Ser Ile Leu Ser

15 Lys Gln Arg Arg Tyr Asn 30 Leu Gly Glu Thr Ile Gly 45 Gly Glu Phe Met His Gln 60 Ser Asn Leu Phe Gly Glu 75 80 Asn Lys Phe Ile Leu Gln 95 Pro Arg Ser Ile Gly Gly 110 Val Gly Asp Met His Arg 125 Ser Asn Arg Wing Arg Leu Arg 140 Thr Leu Val Leu Asn 155 160 Gln Asp Glu Wing Lys Lys 175 Met Ser Met Asp Pro Gly 190 Tyr Val Thr Phe Met Lys 205 Gly Thr Wing Tyr Arg Lys Lys 220 Phe 235 240 Lys Glu Asn Be Glu Glu 255 Be Asn Leu Be Thr Glu 270 Phe Wing Gly His Glu Thr 285 Phe Leu Pro Gly Cys Pro 300 Leu Glu Val Wing Ala 315 320 Trp Asp Asp Tyr Lys Lys 335 Thr Leu Arg Leu Gly Asn 350 Lys Asp Ile Arg Tyr Lys 365 Leu Pro Val Ile Wing Ala 380 Pro Gln Leu Phe Asn Pro 395 400 Gly Wing Gly Thr Wing 415 Pro Phe Gly Gly Gly Pro 430 Leu Glu Met Wing Val Phe 445 Glu Leu Wing Asp Thr Asp 460 Pro Lys Gly Leu Pro Ile 475 480 <211> 1521 <212> DNA < 213> Zea mays <400> 115

atgggcgcca tgatggcctc cataaccagc gagctcctct ctggccctcc tcgccttgta caccaccacc gtcgccaaat cgccgtcaga agaagaagcg gcccaacctg cccccgggcg ggcgaaactt tcggctacct ccgcgcccac ccggccacct cggcatgtcg cacggtacgg gaagatatac cggtcgagcc gtgtcggcgg acgcggggct gaaccgctac atcctgcaga tgcagctacc cgcgcagcat cggcggcatc ctgggcaagt ggcgacgcgc accgcgagat gcgcgctatc tcgctcaact cgcgccgtgc tgctccccga ggtggagcgc cacaccctgc ccctccgacg gcaccttctc cgcccagcac gaagccaaga gcgaagaaca taatgagcat ggaccccggc gaggaggaga tacatcacct tcatgaaggg cgtcgtgtca gcgccgctca tggaaggcgc tcaagtcacg cgcgtccata cttggagtga aggcttgaga agatgagcag ggagaagtca agcgtggagg gccctgaagc aatccaacct gtccaaggaa cagatcctgg ttcgcggggc acgagacttc gtccatggcg ctcgccctcg tgccctaagg ccgtgcaaga actccgggag gagcatctcc ctaagagggg cgtctaaatt gagctgggaa gactacaagg gttataaacg agacattgcg gctcggcaac gtggtcaggt cgagatgtac actacaatgg gtacgacata ccgcgggggt gcggcggtgc acctggactc gtcgctgtac gaggacccca tggaagagca acaacgcgcc aagcagcttc atgccgtacg gccgggtcgg agctggccaa gctggagatg gccatcttcc .ttccggtggg agctggcgga gccggaccag gccttcgttt aagggcctcc cgatcagggt ccagcgggtc gccgacgacc accgagagca caagaggct a <210> 116 <211> 506 <213> PR

tcttccttcc gccacggcac cccgcggatg ccgtgggccg tgttcgggga acgaggggcg ggtccatgct tcctcagctc tggtcctccg agttcacgtt cggagcggct acttcccggg tagagaggaa aggacgacct acctcttgct ccatcttctt tgattgctag aaatggtttt tcctgcaccg ggaaaatcct gccggttcaa gcggcgggcc tgcaccacct accctttcgt aaggccatcg

cttcatcctg ccacccgtgg gcccttggtc cttcatggag gcggacggtg gctgttcgag ggtgctcgtg cgtccgcctc ctcgtggccg taacctgatg gcggctggag cacggcctac gatggaggac tcttggatgg gagcctgctc cctcgaaggg gagacaaagg cacgcagtgt gaaggtcatc gccggttcta cccttggaga gcggctgtgc ggtgctcaac cgacttcccc tagcgttttg

Met Gly Wing Met Met Wing Be Ile Thr Be Glu Leu Leu Phe Phe Leu 1 5 10 15 Pro Phe Ile Leu 20 Leu Wing Leu Leu Wing 25 Leu Tyr Thr Thr 30 Val Wing Lys Cys His 35 Arg Gln Lys Lys 45 Lys Arg Pro Asn Leu 50 Pro Pro Gly Wing Arg 55 Gly Trp Pro Leu Val 60 Gly Glu Thr Phe Gly Tyr Leu Arg Wing His Pro Wing Thr Be Val Gly Arg Phe Met Glu 65 70 75 80 Arg His Val Arg 85 Wing Tyr Gly Lys Ile Tyr 90 Arg Be Ser Leu Phe 95 Gly Glu Arg Thr Val 100 Val Ser As Asa Wing 105 Gly Leu Asn Arg Tyr 110 Ile Leu Gln Asn Glu 115 Gly Arg Leu Phe Glu 120 Cys Ser Tyr Pro Arg 125 Ser Ile Gly Gly Ile 130 Leu Gly Lys Trp Ser 135 Met Leu Val Leu Val 140 Gly Asp Wing His Arg Glu Met Arg Wing Ile Ser Leu Asn Phe Leu Ser Ser Val Arg Leu 145 150 155 160 Arg Wing Val Leu Leu 165 Pro Glu Val Glu Arg 170 His Thr Read Leu Val 175 Read Le Arg Be Trp Pro 180 Be Asp Gly Thr 185 Phe Be Wing Gln His 190 Glu Wing Lys Lys Phe 195 Thr Phe Asn Leu Met 200 Wing Lys Asn Ile Met 20 5 Ser Met Asp Pro Gly 210 Glu Glu Glu Thr Glu 215 Arg Leu Arg Leu Glu 220 Tyr Ile Thr Phe

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1500 1521 Met Lys Gly Val Val Being Pro Wing Read Asn Phe Pro Gly Thr Wing Tyr 225 230 235 240 Trp Lys Wing Leu Lys Be Arg Wing Be Ile Leu Gly Val Ile Glu Arg 245 250 255 Lys Met Wing Glu Asp Arg Leu Glu Lys Be Wing Arg Be Glu Lys Be Val 260 265 270 Glu Glu Asp Winged Leu Gly Trp Wing Lys Gln Be Asn Leu Be 275 280 285 Lys Glu Gln Ile Leu Asp Leu Leu Leu Leu Seru Leu Phe Wing Gly His 290 295 300 Glu Thr Be Ser Met Leu Wing Leu Wing Ile Phe Leu Glu Gly 305 310 315 320 Cys Pro Lys Wing Valu Glu Leu Arg Glu Glu His Read Leu Ile Wing 325 330 335 Arg Arg Gln Arg Read Le Arg Gly Wing Be Lys Read Trp Glu Asp Tyr 340 345 350 Lys Glu Met Val Phe Thr Gln Cys Val Ile Asn Glu Thr Read Le Arg 355 360 365 Gly Asn Val Val Arg Phe Read His Arg Lys Val Ile Arg Asp Val His 370 375 380 Tyr Asn Gly Tyr Asp Ile Pro Arg Gly Trp Lys Ile Leu Pro Val Leu 385 390 395 400 Wing Wing Val His Leu Asp Be Seru Leu Tyr Glu Asp Pro Ser Arg Phe 405 410 415 Asn Pro Trp Arg Trp Lys Ser Asn Asn Ala Pro Be Ser Phe Met Pro 420 425 430 Tyr Gly Gly Gly Pro Arg Leu Cys Wing Gly Be Glu Leu Wing Lys Leu 435 440 445 Glu Met Wing Ile Phe Leu His His Leu Val Leu Asn Phe Arg Trp Glu 450 455 460 Leu Glu Wing Pro Asp Gln Wing Phe Val Tyr Pro Phe Val Asp Phe Pro 465 470 475 480 Lys Gly Leu

<210> 117 <211> 1566 <212> DNA <213> Zea mays <4 00> 117

atgggcgcca tgatggcctc cataaccagc gagctcctct tcttccttcc cttcatcctg 60

ctggccctcc tcgccttgta caccaccacc gtcgccaaat gccacggcac ccaccagtgg 120

cgccgtcaga agaagaagcg gcccaacctg cccccgggcg cccgcggatg gcccttggtc 180

ggcgagactt tcggctacct ccgcgcccac ccggccacct ccgtgggccg cttcatggag 240

cggcatgtcg cacggtacgg gaagatatac cggtcgagcc tgttcgggga gcggacggtg 300

gtgtcggcgg acgcggggct gaaccggtac atcctgcaga acgaggggcg gctgttcgag 360

tgcagctacc cgcgcagcat cggcggcatc ctgggcaagt ggtccatgct ggtgctcgtg 420

ggcgacgcgc accgcgagat gcgcgctatc tcgctcaact tcctcagctc cgtccgcctc 480

cgcgccgtgc tgctccccga ggtggagcgc cacaccctgc tggtcctccg ctcctggccg 540

cctccgaccg gcaccttctc cgcccagcac gaagccaaga agttcacgtt taacctgatg 600

gcgaagaaca taatgagcat ggaccccggc gaggaggaga cggagcggct gcggctggag 660

tacatcacct tcatgaaggg cgtcgtgtca gcgccgctca acttcccggg cacggcctac 720

tggaaggcgc tcaagtcgcg cgcgtccata cttggagtga tagagaggaa gatggaggac 780

aggcttgaga agatgagcag ggagaagtca agcgtggagg aggacgacct tcttggatgg 840

gccctgaagc aatccaacct gtccaaggaa cagatcctgg acctcttgct gagcctgctc 900

ttcgcggggc acgagacttc gtccatggcg ctcgccctcg ccatcttctt cctcgaaggg 960

tgccctaagg ccgtgcaaga actccgggag gagcatctcc tgattgctag gagacaaagg 1020

ctaagggggg cgtccaaatt gagctgggaa gactacaagg aaatggtttt cacgcagtgt 1080

gttataaacg agacattgcg gctcggcaac gtggtcaggt tcctgcaccg gaaggtcatc 1140

cgagatgtac actacaatgg gtacgacata ccgcgggggt ggaaaatcct gccggttcta 1200

gcggcggtgc acctggactc gtcgctgtac gaggatccca gccggttcaa cccttggaga 1260

tggaaggtca gtgccctgcc ccctcccctc gcaaaaggca caagccaagg gacaagcaag 1320 aggtttacac cgttcggtgg tggcccccgg ctctgcccag gatcagagct cgctaaagtg 1380 gagactgctt tcttcctcca tcaccttgtc ctcaattata gatggagaat tgatggcgat 1440 gacattccaa tggcataccc gtatgtggag tttcagagag gtctgccaat agaaatcgag 1500 ccaacgtccc ctgaatttga ctgtcctgga gctacagcca tcagttatca caccagagag 1560 1566 aaatga

<210> 118 <211> 521 <212> PRT

<213> Zea mays <4 00> 118 Met Gly Wing Met Met Wing Be Ile Thr Be Glu Leu Leu Phe Phe Leu 1 5 10 15 Pro Phe Ile Leu Leu Wing Leu Leu Wing Tyr Thr Thr Val Wing 20 25 30 Lys Cys His Gly Thr His Gln Arg Trp Arg Gln Lys Lys Arg Pro 35 40 45 Asn Leu Pro Pro Gly Wing Arg Gly Trp Pro Read Val Gly Glu Thr Phe 50 55 60 Gly Tyr Leu Arg Wing His Pro Thr Be Val Gly Arg Phe Met Glu 65 70 75 80 Arg His Val Arg Wing Arg Tyr Gly Lys Ile Tyr Arg Being Being Read Phe Gly 85 90 95 Glu Arg Thr Val Val Being Wing Asp Wing Gly Leu Asn Arg Tyr Ile Leu 100 105 110 Gln Asn Glu Gly Arg Leu Phe Glu Cys Ser Tyr Pro Arg Ser Ile Gly 115 120 125 Gly Ile Leu Gly Lys Trp Ser Met Leu Val Leu Val Gly Asp Wing His 130 135 140 Arg Glu Met Arg Wing Ile Ser Leu Asn Phe Leu Being Ser Val Arg Leu 145 150 155 160 Arg Wing Val Leu Leu Pro Glu Val Glu Arg His Thr Leu Val Leu 165 170 175 Arg Be Trp Pro Pro Thr Gly Thr Phe Be Wing Gln His Glu Wing 180 185 190 Lys Lys Phe Thr Phe Asn Leu Met Wing Lys Asn Ile Met Being Met Asp 195 200 205 Pro Gly Glu Glu Glu Thr Glu Arg Leu Arg Leu Glu Tyr Ile Thr Phe 210 215 220 Met Lys Gly Val Val Ser Wing Pro Leu Asn Phe Pro Gly Thr Wing Tyr 225 230 235 240 Trp Lys Wing Leu Lys Ser Arg Wing Ser Ile Leu Gly Val Ile Glu Arg 245 250 255 Lys Met Glu Asp Arg Leu Glu Lys Met Ser Arg Glu Lys Ser Ser Val 260 265 270 Glu Glu Asp Asp Leu Gly Trp Wing Leu Lys Gln Ser Asn Leu Ser 275 280 285 Lys Glu Gln Ile Leu Asp Leu Leu Leu Phe Gly Wing His 290 295 300 Glu Thr Be Being Met Wing Leu Wing Leu Wing Ile Phe Phe Leu Wing Glu Gly 305 310 315 320 Cys Pro Lys Val Wing Gln Glu Leu Arg Glu Glu His Leu Ile Wing 325 330 335 Arg Arg Gln Arg Leu Arg Gly Ala Ser Lys Read Trp Glu Asp Tyr 340 345 350 Lys Glu Met Val Val Phe Thr Gln Cys Val Ile Asn Ile Leu Pro Val Leu 385 390 395 400 Wing Val Wing Val His Leu Asp Be Ser Leu Tyr Glu Asp Pro Ser Arg Phe 405 410 415 Asn Pro Trp Arg Trp Lys Val Ser Wing Leu Pro Pro Leu Lys 420 Wing

425

430

Gly Thr Be Gln Gly Thr Be Lys Arg Phe Thr Pro Phe Gly Gly Gly 435 440 445 Pro Arg Read Cys Pro Gly Be Glu Read Wing Lys Val Glu Thr Wing Phe 450 455 460 Phe Read His His Leu Val Read Asn Tyr Arg Trp Arg Ile Asp Gly Asp 465 470 475 480 Asp Ile Pro Met Tyr Wing Pro Tyr Val Glu Phe Gln Arg Gly Leu Pro 485 490 495 Ile Glu Ile Glu Pro Thr Be Pro Glu Phe Asp Cys Pro Gly Wing Thr 500 505 510 Wing His Thr Arg Glu Lys

<210> <211> <212> <213> <220> <221> <222> <223> <400>

515 119 1257 DNA

Brassica

520

rapa

or t

misc_feature (967) .. (969) η is a, c, g 119

atgttcgaaa cagagcatac ctcctcttct tgattctctt aaatctggat ggccatttct actctcggtt acttcatgca ttgtttggag aaccaacgat aacgaaggaa gactctttga tggtcgatgc ttgttcttgt tttctaagtc acgctcgtct ttcgttctta attcttggca tttacgttta atctaatggc gagcagttaa agaaagagta ctcccaggaa ctgcttatcg attgagaaga aaatggaaga gagaaaatca gcagagatta gtagaagtga tcattatgag ggatgggttc taaaacattc caatctttcg ttattatttg ccggacatga tgaaggaaga attgggatga taggaaatgt acgatatccc cccgttacga

gcgggcnnnt tcagaattga actcttcgac tataaaggat ttggataact <210> 120 <211> <212> <213> <220> <221> <222>

tctcgtgcct cttcttcttc tcccatcact tctatctctt 60

gaagagacga agtcgacaca gtttcaatct ccctcctgga 120

aggcgaaacc atcggatatc tcaaacctta ctctgccaaa 180

acaacatatc tccaagtatg ggaagatata tagatcgaat 240

cgtatcagct gatgcaggac tcaacaggtt catattacaa 300

cctcgaagta ttggtgggat tcttgggaaa 360

catagagaca tgagaagtat ctcgctaaac 420

cttcttaagg acgttgagag gcatactttg 480

gttttctctg ctcaagatga ggccaaaaag 540

atgagtatgg atcctggaga agaagagaca 600

atgaaagggg ttgtttctgc tcctctcaat 660

cagcagtcac gagggacgat attgaagttt 720

gagattcaag aagaagacga agaagatgaa 780

agaaaacata gagcagatga tgatcttttg 840

actgagcaaa ttctcgatct tattctcagt 900

gtagccatcg ctctcgctat ctacttttta 960

atcgcgaggg tgaagaagga acttggagag 1020

atggacttta ctcatagtgt tataaatgag 1080

ttgcatcgta aagcactcaa aaacgttcgg 1140

aagtgggtgg aaagtgttac cagtgatctc agccgtacat 1200

cgaacctaat ctctttaatc cttggagatg gcaacag 1257

atgtagttat tggagacatg cagaacgatt acaacattct gaagcatata tgtgactttc taaagctctc

gacatcatct gcatcttgaa ttacaagaca agtaaggttt

419 PRT

Brassica

rapa

INSECURE (323) .. (323)

<223> Xaa can be any naturally occurring amino acid <400> 120

Met Phe Glu Thr Glu His Thr Leu Val Pro Leu Leu Leu Leu Pro 15 10 15

To be

Leu Leu Be Leu Leu Leu Phe Leu Ile Leu Leu Lys Arg Arg Arg Arg 25 25 30 His Ser Phe Asn Leu Pro Gly Lys Pro Gly Trp Pro Phe Leu Gly 35 40 45 Glu Thr Ile Gly Tyr Leu Lys Pro Tyr Ala Lys Thr Leu Gly Tyr 50 55 60 Phe Met Gln Gln His Ile Ser Lys Tyr Gly Lys Ile Tyr Arg Ser Asn 65 70 75 80 Leu Phe Gly Glu Pro Thr Ile Val Ser Wing Asp Wing Gly Leu Asn Arg 85

90

95

Phe Ile Leu Gln Asn Glu Gly Arg Leu Phe Glu Cys Ser Tyr Pro Arg 100 105 110 Ser Ile Gly Gly Ile Leu Gly Lys Trp Being Met Leu Val Leu Val Gly 115 120 125 Asp Met His Arg Phe Leu Be His 130 130 140 Wing Arg Leu Arg Thr Ile Leu Leu Lys Asp Val Glu Arg His Thr Leu 145 150 155 160 Phe Val Leu Asn Ser Trp Gln Gln His Ser Val Phe Ser Wing Gln Asp 165 170 175 Glu Ala Lys Lys Phe Thr Phe Asn Leu Met Lys Wing His Ile Met Ser 180 185 190 Met Asp Pro Gly Glu Glu Glu Thr Glu Leu Lys Lys Glu Tyr Val 195 200 205 Thr Phe Met Lys Gly Val Val Ala Pro Leu Asn Leu Pro Gly Thr 210 215 220 Wing Tyr Arg Lys Wing Leu Gln Gln Ser Arg Gly Thr Ile Leu Lys Phe 225 230 235 240 Ile Glu Lys Lys Met Glu Glu Arg Lys Ser Glu Ile Gln Glu Asu Glu Asp Arg Ser Asp His Tyr Glu Arg Lys 260 265 270 His Arg Wing Asp Asp Asp Leu Leu Gly Trp Val Leu Lys His Ser Asn 275 280 285 Leu Being Thr Glu Gln Ile Leu Asp Leu Ile Leu Being Leu Leu Phe Wing 290 295 300 Gly His Glu Thr Being Val Ala Ile Ala Ile Tyr Phe Leu 305 310 315 320 Wing Gly Xaa Leu Lys Glu Glu His Leu Glu Ile Wing Arg Val Lys Lys 325 330 335 Glu Leu Gly Glu Be Glu Leu Asn Trp Asp Tyr Lys Thr Met Asp 340 345 350 Phe Thr His Ser Val Ile Asn Glu Thr Leu Arg Leu Gly Asn Val Val 355 360 365 Arg Phe Read His Arg Lys Wing Leu Lys Asn Val Arg Tyr Lys Gly Tyr 370 375 380 Asp Ile Pro Be Gly Read Asp Asn Be Arg Tyr Asp Glu Pro Asn

Trp Gln Gln

<210> 121 <211> 848 <212> DNA

<213> Hordeum vulgare <4 00> 121

atggccgcca tgatggcatc cataaccagc gagctccttt tcttcctccc cttcatcctc 60

ctggccctgc tcaccttcta caccagcagc gtggccaaat gccatggcct ccaccggtgg 120

agcggccgga cgaagaagaa gcggccgaat ctgccgcccg gcgccgccgg ctggcctttc 180

gtcggcgaga ccttcgggta cctccgcgcc cacccggcca cctccatcgg ccagttcatg 240

aaccagcaca tcgcacggta cgggaagata taccggtcga gcctgttcgg ggagcggacg 300

gtggtgtcgg cggacgcggg gctgaaccgg tacatcctgc agaacgaggg gcggctgttc 360

gagtgcagct acccgcggag catcggcggc atcctgggca aatggtccat gctggtgctc 420

gtgggcgacc cccaccgcga gatgcgctcc atctccctca acttcctcag ctccctccgc 480

ctccgcgccg tgctcctccc ggaggtggag cgccacaccc tcctcgtcct ccgcgactgg 540

ctgccttcct cctcctccgc cgtcttctcc gcccagcacg aagccaagaa gttcacgttt 600

aacctgatgg cgaagaacat catgagcatg gaccccggcg aggaggagac ggagcggctg 660

aggctcgagt acatcacctt catgaagggg gtggtgtccg cgcccctcaa cttccccggg 720

acggcctact ggaaggccct caagtctcga gccaccatac ttggggtaat agagaggaaa 780

atggaggata ggctcgagaa aatgaacaag gaggcctcga gcatgaagaa gatatctctc 840

ggggggcg 848 <210> 122 <211> 282 <212> PRT

<213> Hordeum vulgare <400> 122

Met Wing Ala Met Met Ala Ser Ile Thr Be Glu Leu Leu Phe Phe Leu 1 5 10 15 Pro Phe Ile Leu 20 Leu Wing Leu Leu Thr 25 Phe Tyr Thr Ser 30 Val Alys Lys Cys His 35 Gly Leu His Arg Trp 40 Ser Gly Arg Thr Lys 45 Lys Lys Arg Pro Asn 50 Leu Pro Pro Gly Wing 55 Wing Gly Trp Pro Phe 60 Val Gly Glu Thr Phe Gly Tyr Leu Arg Wing His Pro Thr Be Ile Gly Gln Phe Met 65 70 75 80 Asn Gln His Ile Wing 85 Arg Tyr Gly Lys Ile 90 Tyr Arg Be Ser Leu 95 Phe Gly Glu Arg Thr 100 Val Val Ser Wing Asp 105 Wing Gly Leu Asn Arg 110 Tyr Ile Leu Gln Asn 115 Glu Gly Arg Leu Phe 120 Glu Cys Ser Tyr Pro 125 Arg Be Ile Gly Gly 130 Ile Leu Gly Lys Trp 135 Ser Met Leu Val Leu 140 Val Gly Asp Pro His Arg Glu Met Arg Be Ile Be Leu Asn Phe Leu Be Ser Leu Arg 145 150 155 160 Leu Arg Wing Val Leu 165 Leu Pro Glu Val Glu 170 Arg His Thr Leu Leu 175 Val Leu Arg Asp Trp 180 Leu Pro Ser Ser Ser 185 Ser Val Val Phe Ser 190 Gln His Glu Wing 195 Lys Lys Phe Thr Phe 200 Asn Leu Met Ala Lys 20 5 Asn Ile Met Be Met 210 Asp Pro Gly Glu Glu 215 Glu Thr Glu Arg Leu 220 Arg Leu Glu Tyr Ile Thr Phe Met Lys Gly Val Val Be Wing Pro Read Asn Phe Pro Gly 225 230 235 240 Thr Wing Tyr Trp Lys 245 Wing Leu Lys Ser Arg 250 Wing Thr Ile Leu Gly 255 Val Ile Glu Arg Lys 260 Met Glu Asp Arg Leu 265 Glu Lys Met Asn Lys 270 Glu Wing To Be Met 275 Lys Lys Ile Ser Leu 280 Gly Gly

<210> 123 <211> 1200 <212> DNA

<213> Lotus japonicus <400> 123

atgtctgact catacctcac tttctgtttt ctttcttcca ttcttgctct cactgtaatc 60

atcattttca tgaaaagaaa gaaggcaagg cttaaccttc cccctggaaa aatgggatgg 120

ccctttcttg gggaaaccat tgggtacttg aagccatact ctgctaccac agtaggagat 180

tttatggaaa agcacatagc aaggtatggt acaatttaca agtcaaaatt gtttggtgag 240

cctgcaatag tttctgcaga tgcagagttg aacaggttca tattacagaa cgaagggaag 300

ctgtttgagt gcagctatcc aagaagcatt ggaggaatac ttggaaaatg gtccatgttg 360

gtcttagttg gggacatgca tagggacatg agaaacattt cactgaactt tctgagcctt 420

gctaggctca aaacacatct attgaaagaa gtggagaagc actctcttct agttctaggc 480

tcttggaaag aaaattgtac attctcagct caagatgaag caaagaagtt cacattcaat 540

ttgatggcga aacatatcat gagcttggat cctgggaatc tagagacaga acagctgaag 600

aaagagtatg tctctttcat gaatggtgtg gtgtctgcac ctttgaattt tcccggaact 660

gcatacagaa aagcattaaa gtctaggtcc accatactga agttcataga gggaaaaatg 720

gaagaaagga tcaaaagaaa ccaaaatttg gaggaagatc ttctaaactg ggttgtgatg 780

cattcaaatc tttcaactga gcaaattctt gacctggttc tgagcttgct ctttgcaggc 840

catgaaactt catctgtggc tatagcttta gctatttact ttttgccagg ttgtcctaaa 900

gctatacaac aattaaggga agaacataga gaaatagcca ggtccaagaa gcaagcaggg 960

gaggttgaat taacttggga tgattacaaa agaatggagt ttactcaatg tgtagttgtg 1020 aatgaaacac tgaggttggg aaatgttgtg aggttccttc acaggaaggc tctgaaagat 1080 gttcggtaca aaggttatga cattccacgt gggtggaaag tcctccctgt gatttcagct 1140 atgcatctgg atcctgcact tttttaccaa cctcaacact tcaatccatg 1200 gagatggaag

<210> 124

<211> 400

<212> PRT

<213> Lotus japonicus

<400> 124

Met Ser Asp Ser Tyr Leu Thr Phe Cys Phe Le Ser Ser Ile Leu Wing 1 5 10 15

Leu Thr Val Ile Ile Ile Phe Met Lys Arg Lys Lys Wing Arg Leu Asn

20 25 30

Read Pro Gly Gly Lys Met Gly Trp Pro Phe Read Gly Gly Glu Thr Ile Gly

35 40 45

Tyr Leu Lys Pro Tyr Be Wing Thr Thr Val Gly Asp Phe Met Glu Lys

50 55 60

His Ile Wing Arg Tyr Gly Thr Ile Tyr Lys Ser Lys Leu Phe Gly Glu 65 70 75 80

Pro Wing Ile Val Ser Wing Asp Wing Glu Leu Asn Arg Phe Ile Leu Gln

85 90 95

Asn Glu Gly Lys Leu Phe Glu Cys Ser Tyr Pro Arg Ser Ile Gly Gly

100 105 110

Ile Leu Gly Lys Trp Being Met Leu Val Leu Val Gly Asp Met His Arg

115 120 125

Asp Met Arg Asn Ile Be Read Asn Phe Read Be Read Read Wing Arg Read Read Lys

130 135 140

Thr His Leu Leu Lys Glu Val Glu Lys His Ser Leu Leu Val Leu Gly 145 150 155 160

Be Trp Lys Glu Asn Cys Thr Phe Be Wing Gln Asp Glu Wing Lys Lys

165 170 175

Phe Thr Phe Asn Le Met Met Wing Lys His Ile Met Being Le Asp Pro Gly

180 185 190

Asn Leu Glu Thr Glu Gln Leu Lys Lys Glu Tyr Val Ser Phe Met Asn

195 200 205

Gly Val Val Ser Wing Pro Read Asn Phe Pro Gly Thr Wing Tyr Arg Lys

210 215 220

Wing Read Lys Be Arg Be Thr Ile Read Lys Phe Ile Glu Gly Lys Met 225 230 235 240

Glu Glu Arg Ile Lys Arg Asn Gln Asn Read Glu Glu Asp Read Leu Asn

245 250 255

Trp Val Val Met His Be Asn Leu Be Thr Glu Gln Ile Leu Asp Leu

260 265 270

Val Leu Ser Leu Leu Phe Ala Gly His Glu Thr Ser Ser Val Ala Ile

275 280 285

Wing Leu Wing Ile Tyr Phe Leu Pro Gly Cys Pro Lys Wing Ile Gln Gln

290 295 300

Read Arg Glu Glu His Arg Glu Ile Wing Arg Be Lys Lys Wing Gln Gly 305 310 315 320

Glu Val Glu Leu Thr Trp Asp Asp Tyr Lys Arg Met Glu Phe Thr Gln

325 330 335

Cys Val Val Val Val Asn Glu Thr Leu Arg Leu Gly Asn Val Val Arg Phe

340 345 350

Read His Arg Lys Wing Read Le Lys Asp Val Arg Tyr Lys Gly Tyr Asp Ile

355 360 365

Pro Arg Gly Trp Lys Val Leu Pro Val Ile Be Wing Met His Leu Asp

370 375 380

Pro Wing Read Phe Tyr Gln Pro Gln His Phe Asn Pro Trp Arg Trp Lys 385 390 395 400

<210> 125

<211> 1092

<212> DNA

<213> Malus domesticus

<400> 125 agagacatga atgagagaag ttttcagctc agcttggatc aaaggtgtgg tcaagatcaa accgaaaaca aaggagcaaa gtgtctatag agggaagaac tgggaggact gggaatgtgg gacattccat ctttttgacc caccgtggat ccatttgggg gtgttcatcc ccttttgctt gtaccaaact <210> 126 <211> 363 <212> PRT <213> Malus <400> 126

ggatgatatc ttgaaaagca aggatgaagc ctggaaaacc tttctcctcc cgattctgaa ttggggaaga ttcttgactt ccttagcaat acagtgaaat acaagaaaat tgagattttt ctgggtggaa acccgacaca catgggact tcggacaca

domestic

tctcaacttc cactcttctt taagaagttc agagactgag tctgaattta gtttatagag tgatctgctt aatattgagc ttacttttta tgccaaagcc tgccaaagcc

ttgagccatg gttttgggga acgttcaact cagctgaaga ccaggaacag tgcaaaatga ggatgggttc ttgctctttg ccaagctgcc aagaaactgg gaattgtgag

ccagactcag gttggaagga tgatggccaa aattgtatgt cttacagaag aagaaagatt tgaagaattc ctggccatga ctaatgcaat caggcgagac tctgtgagac

aacccatctg aaactctgtt acatatcatg tactttcatg agccttacag gatggaggga aaatctttca aacttcatca tctgcagtta agagttgaat acttcggctt caaagggtat ggatccttta caaccaccac caactttatg tgaagggccca

Arg Asp Met Arg Met Ile Be Leu Asn Phe Leu Be His Wing Arg Leu 1 5 10 15 Arg Thr His Leu Met Arg Glu Val Glu Lys His Thr Leu Leu Val Leu 20 25 30 Gly Ser Trp Lys Glu Asn Be Val Phe Ser Ala Gln Asp Glu Ala Lys 35 40 45 Lys Phe Thr Phe Asn Leu Met Ala Lys His Ile Met Being Read Asp Pro 50 55 60 Gly Lys Pro Glu Thr Glu Gln Leu Lys Lys Leu Tyr Val Thr Phe Met 65 70 75 80 Lys Gly Val Val Ser Pro Pro Leu Asn Leu Pro Gly Thr Wing Tyr Arg 85 90 95 Arg Wing Leu Gln Ser Arg Be Thr Ile Leu Lys Phe Ile Glu Cys Lys 100 105 110 Met Lys Glu Arg Leu Met Glu Gly Thr Glu Asn Ile Gly Glu Asp Asp 115 120 125 Leu Leu Gly Trp Val Leu Lys Asn Ser Asn Leu Ser Lys Glu Gln Ile 130 135 140 Leu Asp Leu Ile Leu Seru Leu Phe Wing Gly His Glu Thr Ser Ser 145 150 155 160 Val Ser Ile Ala Leu Ala Ile Tyr Phe Leu Pro Be Cys Pro Asn Wing 165 170 175 Ile Leu Gln Leu Arg Glu Glu His Se r Glu Ile Wing Lys Wing Lys Lys 180 185 190 Leu Wing Gly Glu Thr Glu Leu Asn Trp Glu Asp Tyr Lys Lys Met Glu 195 200 205 Phe Thr Glu Cys Val Ile Cys Glu Thr Leu Arg Leu Gly Asn Val Val 210 215 220 Arg Phe Leu His Arg Lys Wing Leu Lys Asp Val Arg Tyr Lys Gly Tyr 225 230 235 240 Asp Ile Pro Be Gly Trp Lys Val Leu Pro Val Ile Wing Val His 245 250 255 Leu Asp Pro Leu Phe Asp His Pro Gln His Phe Asn Pro Trp Arg 260 265 270 Trp Gln Gln Asn Asn Asn His His Arg His Gly Be Ser Ser Ser Ser Ser 275 280 285 Ser Cyr Tyr Thr Ser Met Thr Ser As As Phe Met Pro Phe Gly Gly 290 295 300 Gly Pro Arg Leu Cys Wing Gly Ser Glu Leu Wing Lys Leu Glu Met Wing

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1092 305 310 315

Val Phe Ile His His Leu Val Leu Asn Phe His

325 330

Pro Phe Asp Lys Pro Phe Ala Phe Pro Phe Val

340 345

Read Pro Ile Thr Wing His Arg Tyr Val Pro Asn

355 360

<210> 127 <211> 748 <212> DNA

<213> Vitis vinifera <4 00> 127

agacttcatg gagcaacaca tatcaaggtt cggagaaatc cgagccaacc atagtctcag cggattctgg gctgaacaga aaaattgttt gaatgcagct atcccagaag cataggtgga gctggtttta gttggagaca tgcatagaga catgagaacc ccatggcagg cttaggactc atctcctacc agaggtggtg aagctcttgg aaggagaatt gtacattttc tgctcaagat caatctgatg gcaaaacata tcatgagctt ggatcccgga taagaaagag tatgttactt tcatgaaagg agtggtatct aactgcatac agaaaagccc tacagtctcg gtccaccatc gatggaagag aggattcaga aactgagggg aggagggttt tcttcttgga tgggtgctga agcattccaa cctttcaact actgagcttg ctctttgctg gccatgaaac ttcatcagtg cttcttggaa ggctgtccca acgccgtt <210> 128 <211> 248 <212> PRT

<213> Vitis vinifera <4 00> 128

Asp Phe Met Glu Gln His Ile Be Arg Phe Gly 15 10

Asn Leu Phe Gly Glu Pro Thr Ile Val Ser Ala

20 25

Arg Phe Ile Leu Gln Asn Glu Gly Lys Leu Phe

35 40

Arg Be Ile Gly Gly Ile Read Gly Lys Trp Ser

50 55

Gly Asp Met His Arg Asp Met Arg Thr Ile Ser 65 70 75

His Gly Arg Leu Arg Thr His Leu Leu Pro Glu

85 90

Leu Leu Val Leu Being Trp Lys Glu Asn Cys

100 105

Asp Glu Wing Lys Lys Phe Thr Phe Asn Leu Met

115 120

Get Asp Pro Gly Lys Pro Glu Thr Glu Gln

130 135

Val Thr Phe Met Lys Gly Val Val Ser Wing Pro 145 150 155

Thr Wing Tyr Arg Lys Wing Read Gln Ser Arg Ser

165 170

Ile Glu Read Lys Met Glu Arg Ile Glu Lys

180 185

Phe Glu Asn Met Glu Asp Asp Asp Read Leu Gly

195 200

Be Asn Leu Be Thr Glu Gln Ile Leu Asp Leu

210 215

Phe Ala Gly His Glu Thr Ser Ser Val Ala Ile 225 230 235

Phe Leu Glu Gly Cys Pro Asn Wing 245

<210> 129

320

Trp Glu Leu Wing Asp 335

Asp Phe Gln Asn Gly 350

tacaagtcca ttcatactac attcttggga atctccctca aagcacactt gaagctaaga aagccggaga gctcctttga ttaaaattta gagaatatgg gagcaaatcc gcaatagctt

atctgtttgg agaacgaagg aatggtccat acttcttgag tgcttgttct agtttacctt ctgagcagct atttccctgg tcgagctgaa aggatgacga tggacttggt tagctatcta

Glu

Asp

Glu

Met 60 Leu

Val

Thr

Allah

Leu 140 Leu

Thr

Read

Trp

Val 220 Wing

Ile

To be

Cys

45

Read

Asn

Val

Phe

Lys 125 Lys

Asn

Ile

Arg

Val 205 Leu

Read

Tyr

Gly

30

To be

Val

Phe

Lys

Ser 110 His

Lys

Phe

Read

Gly 190 Leu

To be

Allah

Lys Ser 15

Read asn

Tyr pro

Read val

Read Ser 80 His Thr 95

Gln Wing

Ile met

Glu Tyr

Pro Gly 160 Lys Phe 175

Gly Gly

Lys his

Leu Leu

Ile Tyr 240

60 120 180 240 300 360 420 480 540 600 660 720 748 <211> 1737 <212> DNA

<213> Arabidopsis thaliana <400> 129

atgcaacaac tgtcaacttc tctactaaaa acacgagttc catggcctta aagatatctc aacatgtcat tctataatac ccaccacttt tccggaattt agttctccaa agtccactgt gaaggaaagt tacgtaagat tcaagactgt ctgctgattc aatgtgaaca acgcttccaa tctgtaacac ttcggaaggg ggggaacctt ttgatgattc aatgatcaag aagacgaaac acaattggtg tttcggaaat tcatacatgt acaggtgtca tataatacag gatgggttct ttagaggctg aaaaggcatt atggatatat actctacggt gctcaggaac taatatcaac tgctatagct tgcaaaagga ctgaatccaa gatttgcata gattttgaga acggaatgaa aacgcatggt acgggcttgg catcacttca gaatggcttt gggacatctt tgcatgcctt atagtagcag atagaaaaaa ttagaaagat tagatgaagc gagagcagcg tttacgcttt gccatgcttc atttcggtct ataaaggctg caatggagaa <210> 130 <211> 578 <212> PRT <213> Arabidopsis thaliana <400> 130

Met Gln Gln Read Be Thr Be Read Gly Read Asn Thr Tyr Asn Glu Glu 1 5 10 15 Arg Asn Be Thr 20 Be Thr Lys Asn Thr 25 Be Be Glu Asp Tyr 30 Be Pro Arg Gln Be 35 Lys His Thr Gln Be 40 His Gly Leu Lys Asp 45 Ile Being Gly Asn Phe 50 His Being His Gly Val 55 Asn Gly Gly Val Being 60 Asn Met Being Phe Tyr Asn Thr Pro Being Val Wing Ward Gln Leu Being Gly Ile Wing Pro 65 70 75 80 Pro Pro Leu Phe Arg 85 Asn Phe Gln Pro Wing 90 Val Wing Asn Pro Asn 95 Ser Leu Ile Thr Asp 100 Ser Ser Pro Lys Ser 105 Thr Val Asn Ser Thr 110 Leu Gln Ala Pro Arg 115 Arg Lys Phe Val Asp 120 Glu Gly Lys Leu Arg 125 Lys Ile Be Gly Arg 130 Read Le Phe Be Asp Be 135 Gly Pro Arg Arg Be 140 Be Arg Read Le Be Wing Asp Be Gly Wing Asn Ile Asn Be Be Val Wing Thr Val Be Gly 145 150 155 160 Asn Val Asn Asn Wing 165 Ser Lys Tyr Leu Gly 170 Gly Ser Lys Leu Ser 175 Ser Leu Wing Leu Arg 180 Ser Val Thr Leu Arg 185 Lys Gly His Ser Trp 190 Wing Asn Glu Asn Met Asp Glu Gly Val Arg Gly Glu Pro Phe Asp Asp Ser Ar g

cctcggctta tgaagattat cggaaatttc gccttcgcca tcagccagct taactctact ttctggcaga aggggcaaac gtatttggga acactcctgg aaggcctaat aatgtcgatt tttaaacctc ggaggcactg ttcccaggtc ccgtcttgcc cctctatcat cgatcgctta ccatgagacc tgcacatacc aagttaccaa aatgatatat cctaataaac gaagagaagt ccctcttcca tctagaagtt aatgggcagg agctttagat attgcatgtt

aacacttaca agtccaaggc cattctcatg gtggctgcac gttgcaaacc cttcaagcac ctattttctg attaattcaa ggttctaaat gcaaatgaaa actgcctcaa ggtggcatag cttaggacac gatacgtata gggaaagcat cgtctggctt ttgaaggaag gctcctcaat gcactgaaga ttatgtggcc aacgcacttc ctacgccaag ccgagttcct gaggaagcac atgtaccaga cttgaggagc atctataagc atgaaaccgc ccagatgaga

acgaggaacg agtctaaaca gagttaatgg agctatccgg caaactccct ctagaagaaa attctggtcc gtgttgcaac tgagttcttt acatggatga cgactggttc caatgagttc tcggagaagg tgaaacttcc actttgaact ctccttattg acatgaagct cttggtgtgc atttcctacg acgaatacac gtgtagatac agaagttaga ctgttataat tagagataat aagctaacat tcaaagagta ggcgaaacat ctgcaactga tcgatgagag

taattcaact 60 cacacaaagc 120 aggtgtttcg 180 tatagctcca 240 tattactgac 300 gtttgtagat 360 acgacggagt 420 agtaagcgga 480 ggcacttcgt 540 aggggtccgt 600 tatggcttcc 660 tcaaacaatc 720 gtgtagactt 780 acataagcat 840 aattgactat 900 cttagaagga 960 gagttacttg 1020 tatgggaaat 1080 agctgttcaa 1140 aactcttgag 1200 aagacactac 1260 gttctcagag 1320 gtcttattta 1380 ggagcaagcc 1440 acttgtctgc 1500 tgcgccttca 1560 gcacgataaa 1620 cgttgctgca 1680 cccgtga 1737 195 200 205

Pro Asn Thr Wing Be Thr Thr Gly Be Met Wing Be Asn Asp Gln Glu

210 215 220

Asp Glu Thr Met Being Ile Gly Gly Ile Wing Met Being Being Gln Thr Ile 225 230 235 240

Thr Ile Gly Val Ser Glu Ile Leu Asn Leu Leu Arg Thr Leu Gly Glu

245 250 255

Gly Cys Arg Read Tyr Met Tyr Arg Cys Gln Glu Wing Read Asp Thr

260 265 270

Tyr Met Lys Leu For His Lys His Tyr Asn Thr Gly Trp Val Leu Ser

275 280 285

Gln Val Gly Lys Wing Tyr Phe Glu Leu Ile Asp Tyr Leu Glu Wing Glu

290 295 300

Lys Wing Phe Arg Wing Read Wing Wing Wing Wing Wing Wing Wing Be Pro Tyr Cys Winged Glu Gly 305 310 315 320

Met Asp Ile Tyr Be Thr Val Leu Tyr His Leu Lys Glu Asp Met Lys

325 330 335

Leu Ser Tyr Leu Wing Gln Glu Leu Ile Be Thr Asp Arg Leu Wing Pro

340 345 350

Gln Being Trp Cys Ala Met Gly Asn Cys Tyr Being Read Gln Lys Asp His

355 360 365

Glu Thr Wing Leu Lys Asn Phe Leu Arg Wing Val Val Gln Leu Asn Pro Arg

370 375 380

Phe Ala Tyr Ala His Thr Read Cys Gly His Glu Tyr Thr Thr Leu Glu 385 390 395 400

Asp Phe Glu Asn Gly Met Lys Ser Tyr Gln Asn Wing Leu Arg Val Asp

405 410 415

Thr Arg His Tyr Asn Ala Trp Tyr Gly Leu Gly Met Ile Tyr Leu Arg

420 425 430

Glu Glu Lys Leu Glu Phe Be Glu His His Phe Arg Met Wing Phe Leu

435 440 445

Ile Asn Pro Be Ser Be Val Ile Met Be Tyr Leu Gly Thr Be Leu

450 455 460

His Wing Read Lys Arg Be Glu Glu Wing Read Leu Glu Ile Met Glu Gln Wing 465 470 475 480

Ile Val Wing Asp Arg Lys Asn Pro Read Le Pro Met Tyr Gln Lys Wing Asn

485 490 495

Ile Leu Val Cys Leu Glu Arg Leu Asp Glu Wing Leu Glu Val Leu Glu

500 505 510

Glu Leu Lys Glu Tyr Wing Pro To Be Glu To Be Val Tyr Wing Leu Met

515 520 525

Gly Arg Ile Tyr Lys Arg Arg Asn Met His Asp Lys Ala Met Leu His

530 535 540

Phe Gly Leu Wing Read Asp Met Lys Pro Pro Wing Thr Asp Val Wing Wing 545 550 555 560

Ile Lys Ala Wing Met Glu Lys Read His Val Pro Asp Glu Ile Asp Glu 565 570 575

To be pro

<210> 131 <211> 2235 <212> DNA

<213> Arabidopsis thaliana <4 00> 131

atggaagcta tgcttgtgga ctgtgtaaac aacagtcttc gtcattttgt ctacaaaaat 60

gctattttca tgtgcgagcg tctctgcgct gagtttcctt ctgaggttaa tttgcagcta 120

ttagccacca gctacctgca gaataatcaa gcttacagtg catatcatct gctaaaggga 180

acacaaatgg ctcagtcccg atacttgttc gcattatcat gcttccagat ggaccttctc 240

aatgaagctg aatctgcact ctgccctgtt aatgaacctg gtgcggagat cccaaatggt 300

gcagcaggcc attaccttct tggacttatt tacaagtata ctgatagaag gaagaatgct 360

gctcaacaat ttaaacagtc cttgacaata gccctctac tttgggctgc atatgaggaa 420

ttatgtatat taggtgctgc tgaggaagca actgcagttt ttggtgaaac agctgctctc 480

tccattcaaa agcagtatat gcaacaactg tcaacttccc tcggcttaaa cacttacaac 540 gaggaacgta attcaacttc tactaaaaac acgagttctg aagattatag tccaaggcag 600

tctaaacaca cacaaagcca tggccttaaa gatatctccg gaaatttcca ttctcatgga 660

gttaatggag gtgtttcgaa catgtcattc tataatacgc cttcgccagt ggctgcacag 720

ctatccggta tagctccacc accacttttc cggaattttc agccagctgt tgcaaaccca 780

aactccctta ttactgacag ttctccaaag tccactgtta actctactct tcaagcacct 840

agaagaaagt ttgtagatga aggaaagtta cgtaagattt ctggcagact attttctgat 900

tctggtccac gacggagttc aagactgtct gctgattcag gggcaaacat taattcaagt 960

gttgcaacag taagcggaaa tgtgaacaac gcttccaagt atttgggagg ttctaaattg 1020

agttctttgg cacttcgttc tgtaacactt cggaagggac actcctgggc aaatgaaaac 1080

atggatgaag gggtccgtgg ggaacctttt gatgattcaa ggcctaatac tgcctcaacg 1140

actggttcta tggcttccaa tgatcaagaa gacgaaacaa tgtcgattgg tggcatagca 1200

atgagttctc aaacaatcac aattggtgtt tcggaaattt taaacctcct taggacactc 1260

ggagaagggt gtagactttc atacatgtac aggtgtcagg aggcactgga tacgtatatg 1320

aaacttccac ataagcatta taatacagga tgggttcttt cccaggtcgg gaaagcatac 1380

tttgaactaa ttgactattt agaggctgaa aaggcattcc gtcttgcccg tctggcttct 1440

ccttattgct tagaaggaat ggatatatac tctacggtcc tctatcattt gaaggaagac 1500

atgaagctga gttacttggc tcaggaacta atatcaaccg atcgcttagc tcctcaatct 1560

tggtgtgcta tgggaaattg ctatagcttg caaaaggacc atgagaccgc actgaagaat 1620

ttcctacgag ctgttcaact gaatccaaga tttgcatatg cacatacctt atgtggccac 1680

gaatacacaa ctcttgagga ttttgagaac ggaatgaaaa gttaccaaaa cgcacttcgt 1740

gtagatacaa gacactacaa cgcatggtac gggcttggaa tgatatatct acgccaagag 1800

aagttagagt tctcagagca tcacttcaga atggctttcc taataaaccc gagttcctct 1860

gttataatgt cttatttagg gacatctttg catgccttga agagaagtga ggaagcacta 1920

gagataatgg agcaagccat agtagcagat agaaaaaacc ctcttccaat gtaccagaaa 1980

gctaacatac ttgtctgctt agaaagatta gatgaagctc tagaagttct tgaggagctc 2040

aaagagtatg cgccttcaga gagcagcgtt tacgctttaa tgggcaggat ctataagcgg 2100

cgaaacatgc acgataaagc catgcttcat ttcggtctag ctttagatat gaaaccgcct 2160

gcaactgacg ttgctgcaat aaaggctgca atggagaaat tgcatgttcc agatgagatc 2220

gatgagagcc cgtga 2235

<210> 132 <211> 744 <212> PRT <213> Arabidopsis thaliana <400> 132 Met Glu Ala Met Leu Val Asp Cys Val Asn Asn Ser Leu Arg His Phe 1 5 10 15 Val Tyr Lys Asn Ala Ile Phe Met Cys Glu Arg Leu Cys Wing Glu Phe 20 25 30 Pro Ser Glu Val Asn Leu Gln Leu Leu Wing Thr Be Tyr Leu Gln Asn 35 40 45 Asn Gln Wing Tyr Ser Wing Tyr His Leu Lys Gly Thr Gln Met Wing 50 55 60 Gln Ser Arg Tyr Leu Phe Wing Leu Be Cys Phe Gln Met Asp Leu Leu 65 70 75 80 Asn Glu Wing Glu Be Wing Leu Cys Pro Val Asn Glu Pro Gly Wing Glu 85 90 95 Ile Pro Asn Gly Wing Gly His Tyr Leu Leu Gly Leu Ile Tyr Lys 100 105 110 Tyr Thr Asp Arg Arg Lys Asn Wing Wing Gln Gln Phe Lys Gln Be Leu 115 120 125 Thr Ile Asp Pro Leu Read Trp Wing Wing Tyr Glu Glu Leu Cys Ile Leu 130 135 140 Gly Wing Wing Glu Glu Wing Thr Wing Val Phe Gly Glu Thr Wing Wing Leu 145 150 155 160 Ser Ile Gln Lys Gln Tyr Met Gln Gln Read Be Thr Be Read Gly Leu 165 170 175 Asn Thr Tyr Asn Glu Glu Arg Asn Be Thr Be Thr Lys Asn Thr Ser 180 185 190 Be Glu Asp Tyr Be Pro Arg Gln Ser Lys His Thr Gln Be His Gly 195 200 205 Leu Lys Asp Ile Be Gly Asn Phe His Be His Gly Val Asn Gly Gly 210 215 220 Val Be Asn Met Be Phe Tyr Asn Thr Pro Be Val Wing Ala Gln 70 225 230 235 240 Leu Ser Gly Ile Ala Pro Pro Leu Phe Arg Asn Phe Gln Pro Ala 245 250 255 Val Asn Pro Wing Asn Be Leu Ile Thr Asp Ser Pro Lys Be Thr 260 265 270 Val Asn Ser Thr Leu Gln Ala Pro Arg Arg Lys Phe Val Asp Glu Gly 275 280 285 Lys Read Arg Lys Ile Be Gly Arg Read Le Phe Be Asp Be Gly Pro Arg 290 295 300 Arg Be Be Arg Leu Be Wing Asp Be Gly Wing Asn Ile Asn Be 305 310 315 320 Val Wing Thr Thr Val Ser Gly Asn Val Asn Asn Wing Ser Lys Tyr Leu Gly 3 25 330 335 Gly Be Lys Read Le Be Be Read Ala Leu Arg Be Val Thr Leu Arg Lys 340 345 350 350 Gly His Be Trp Wing Asn Glu Asn Met Asp Glu Gly Val Arg Gly Glu 355 360 365 Pro Phe Asp Asp Be Arg Pro Asn Thr Wing Be Thr Thr Gly Be Met 370 375 380 Wing Be Asn Asp Gln Glu Asp Glu Thr Met Be Ile Gly Gly Ile Wing 385 390 395 400 Met Be Ser Gln Thr Ile Gly Val Be Glu Ile Leu Asn Leu 405 410 415 Leu Arg Thr Read Gly Glu Gly Cys Arg Read Le Be Tyr Met Tyr Arg Cys 420 425 430 Gln Glu Wing Read Asp Thr Tyr Met Lys Leu Pro His Lys His Tyr Asn 435 440 445 Thr Gly Trp Val Leu Be Gln Val Gly Lys Wing Tyr Phe Glu Leu Ile 450 455 460 Asp Tyr Leu Glu Wing Glu Lys Wing Phe Arg Leu Wing Arg Leu Wing Ser 465 470 475 480 Pro Tyr Cys Leu Glu Gly Met Asp Ile Tyr Ser Thr Val Leu Tyr His 485 490 495 Leu Lys Glu Asp Met Lys Leu Ser Tyr Leu Wing Gln Glu Leu Ile Ser 500 505 5 10 Thr Asp Arg Leu Wing Pro Gln Ser Trp Cys Wing Met Gly Asn Cys Tyr 515 520 525 Ser Read Gln Lys Asp His Glu Thr Wing Leu Lys Asn Phe Leu Arg Wing 530 535 540 Val Gln Leu Asn Pro Arg Phe Wing Tyr Ala His Thr Leu Cys Gly His 545 550 555 560 Glu Tyr Thr Thr Leu Glu Asp Phe Glu Asn Gly Met Lys Ser Tyr Gln 565 570 575 Asn Wing Leu Arg Val Asp Thr Arg His Tyr Asn Wing Trp Tyr Gly Leu 580 585 590 Gly Met Ile Tyr Leu Arg Gln Glu Lys Leu Glu Phe Be Glu His His 595 600 605 Phe Arg Met Ala Phe Leu Ile Asn Pro To Be Ser Val Ile Met Ser 610 615 620 Tyr Leu Gly Thr Be Leu Lys Arg Be Glu Glu Wing Leu 625 630 635 640 Glu Ile Met Glu Gln Wing Ile Val Wing Asp Arg Lys Asn Pro Leu Pro 645 650 655 Met Tyr Gln Lys Wing Asn Ile Leu Val Cys Leu Glu Arg Leu Asp Glu 660 665 670 Wing Leu Glu Val Leu Glu Glu Read Lys Glu Tyr Wing Pro Be Glu Ser 675 680 685 Ser Val Ty r Wing Leu Met Gly Arg Ile Tyr Lys Arg Arg Asn Met His 690 695 700 Asp Lys Wing Met Leu His Phe Gly Leu Wing Leu Asp Met Lys Pro 705 710 715 720 Wing Thr Asp Val Wing Wing Ile Lys Wing Wing Met Glu Lys Wing Read his val

725 730 735 Pro Asp Glu Ile Asp Glu Ser Pro 740

<210> 133 <211> 2181 <212> DNA

<213> Arabidopsis thaliana <400> 133

atgatggaga atctactggc gaattgtgtc cagaaaaacc aatgctatct tcctttgcga acttcttctc gcccaatttc ttgttagcca ggtgttactt gagtaacagt caagcttata ggttcaaaaa cgcctcagtc tcggtattta tttgcattct cttggagagg ctgaagctgc attgttgccc tgtgaagatt ggtgcagctg ggcattatct tcttggtctt atatatagat tcaatacaac agtttaggat ggcattgtca tttgatccat gaactttgta gtttaggtgc cgctgaagaa gcctcaacag cagcgtctta aaacttgtgt agaacaaaga ataagcttct cagattacag attctgataa ggccttaaaa gatacaggtt ccaggagaga accaacaaga tctgaaaatt atgcagcagc actgacaggc aacttagtac aaacggatgg gacttgaaca caggtaatgg atgctccacc gcctctgctt cttaagaata ggatctttga tgtctgtaca tggagtgcgt gtgcgtcgaa ttgtcagcag aggctcaaga agaatctggg cgccgccgta aaaaagaatc ctatgtcgca gtcatttgga aaagattccc tccgagtcaa actatgcacc ttctctttcc tcgatgattg agcaaagaag cgattcctga taccgttact ctaaatgatc tctgtaagtg acactggaag ctctgttgat gatgaggaaa tccccggatc gtttcagcct tatttctgga atttcagaag cttggagatg gccacaggca tttacatatg tacaagtgtc caaaagctat ctcagaaaca atacaataca cactgggttc tattttgagc tacaagacta cttcaacgct gactcttcct tatccttatg ctttggaagg aatggataca tactccactg gagatgaggt tgggctatct ggctcaggaa ctgatttcag tcctggtgtg cagttgggaa ctgttacagt ttgcgtaagg atgtttcaga gagctatcca actgaatgaa agattcacat cacgagtttg ccgcattgga agaattcgag gatgcagaga ggcatagata cgagacacta taatgcatgg tacggtcttg gagaaattcg agtttgcgca gcatcaattt caactggctc tcagtcatca tgtgttacta tggaattgct ttgcatgagt ttgatgatga tggagaaggc tgtactcact gatgcaaaga aaggctcaca tattaaccag cctaggtgat tatcacaaag ctcaaagaat gtgctcctca agaaagcagt gtccatgcat cagctaaagc aatacgacaa agccgtgtta catttcggca tctccatctg atgctgtcaa gataaaggct tacatggaga ctggtgacgg aggaaaattt g <210> 134 <211> 728 <212> PRT

<213> Arabidopsis thaliana <400> 134

Met Met Glu Asn Leu Read Leu Wing Asn Cys Val Gln 15 10

Phe Met Phe Thr Asn Wing Ile Phe Leu Cys Glu

20 25

Phe Pro Ser Glu Val Asn Leu Gln Leu Leu Wing

35 40

Asn Ser Gln Wing Tyr Be Wing Tyr Tyr Ile Leu

50 55

Pro Gln Ser Arg Tyr Read Phe Ala Phe Ser Cys 65 70 75

Leu Gly Glu Wing Glu Wing Wing Leu Leu Pro Cys

85 90

Glu Val Pro Gly Gly Wing Gly Wing His Tyr Leu 100 105

ttaaccattt tatgttcacc 60 catctgaggt gaacctgcaa 120 gtgcatatta tatccttaaa 180 catgctttaa gttggatctt 240 atgctgaaga agttcctggt 300 attctgggag gaagaactgt 360 tgtgttggga agcatatgga 420 ttttcgggaa tgttgcttcc 480 cagaaggagc aaccatagac 540 tatcgcaaac agaacacatt 600 ctggagatat tccaccaaat 660 caccttctcc agtgctttta 720 tgcgtcgtcc agcagtggaa 780 gaaacttttt tagtgaagaa 840 gtgctagaat agcagcaagg 900 attggttaca tctttcacct 960 gaaaatgcag aatccaaagc 1020 cagcaacgac gtcaggccag 1080 agtcaaatcc tagtgaatct 1140 tgctaggcat tctgaaaatt 1200 aggaagcttt gttggcatat 1260 tcatgcaggt tggaaaagca 1320 ttactcttgc tcatcaaaag 1380 ttctttatca cctgaaagaa 1440 ttgatcgcct gtctccagaa 1500 atcatgatac tgctctcaaa 1560 atgcacatac cctttgtggc 1620 gatgctaccg gaaggctctg 1680 gaatgaccta tcttcgtcag 1740 tccaaataaa tccaagatct 1800 caaagagaaa cgatgaggcg 1860 atccgctccc caagtactac 1920 cacagaaagt tttagaagag 1980 cgcttggcaa aatatacaat 2040 ttgctttgga tttaagccct 2100 ggttgatact accagacgag 2160 2181

Lys asn

Leu Leu

Arg Cys 45

Lys Gly 60

Phe Lys Glu Asp Leu Gly

Leu Asn His 15 Leu Wing Gln 30 Tyr Leu Ser Ser Lys Thr Leu Asp Leu 80 Tyr Ala Glu 95 Leu Ile Tyr 110 72 Arg Tyr Ser Gly Arg Lys Asn Cys Ser Ile Gln Phe Arg Met Ala 115 120 125 Leu Ser Phe Asp Pro Leu Cys Trp Glu Wing Tyr Gly Glu Leu Cys Ser 130 135 140 Leu Gly Wing Glu Glu Wing Ser Thr Val Phe Gly Asn Val Wing Ser 145 150 155 160 Gln Arg Leu Gln Lys Thr Cys Val Glu Gln Arg Ile Be Phe Ser Glu 165 170 175 Gly Ala Thr Ile Asp Gln Ile Thr Asp Ser Asp Lys Ala Leu Lys Asp 180 185 190 Thr Gly Leu Be Gln Thr His Ile Pro Gly Glu Asn Gln Gln Asp 195 200 205 Leu Lys Ile Met Gln Pro Gly Asp Ile Pro Pro Asn Thr Asp Arg 210 215 220 Gln Read Be Thr Asn Gly Trp Asp Read Asn Thr Pro Be Pro Val Leu 225 230 235 240 Read Gln Val Met Asp Wing Read Pro Pro Read Leu Lys Asn Met Arg 245 250 255 Arg Pro Wing Val Glu Gly Be Read Met Met Be Val His Gly Val 265 270 Arg Arg Arg Asn Phe Phe Be Glu Glu Read Le Be Glu Wing Wing Gln Glu 275 280 285 Glu Be Gly Arg Arg Arg Be Wing Arg Ile Wing Arg Lys Lys Asn 290 295 300 Pro Met Be Gln Be Phe Gly Lys Asp Ser His Trp Read His Read Le 305 310 315 320 Pro Be Le Glu Asn Tyr Ala Pro Le Le Be Met Ile Gly Lys 325 330 335 Cys Arg Ile Gln Be Lys Glu Val Ile Pro Asp Thr Val Thr Leu 340 345 350 Asn Asp Pro Wing Thr Thr Be Gly Gln Be Val Asp Ile Gly Be 355 360 365 Be Val Asp Asp Glu Glu Lys Be Asn Pro Be Glu Be Pro Asp 370 375 380 Arg Phe Be Read Ile Be Gly Ile Be Glu Val Leu Be Read Leu Lys 385 390 395 400 Ile Leu Gly Asp Gly His Arg His Leu His Met Tyr Lys Cys Gln Glu 405 410 415 Wing Leu Leu Wing Tyr Gln Lys Leu Being Gln Lys Gln Tyr Asn Thr His 420 425 430 Trp Val Leu Met Gln Val Gly Lys Wing Tyr Phe Glu Read Gln Asp Tyr 435 440 445 Phe Asn Asp Wing Asp Be Being Phe Thr Winged His Gln Lys Tyr Pro Tyr 450 455 460 Winged Leu Glu Gly Met Asp Thr Tyr's Winged Val Leu Tyr His Leu Lys 465 470 475 480 Glu Glu Met Winged Gly Tyr Leu Wing Gln Glu Leu Ile Be Val Asp 485 490 495 Arg Leu Be Pro Glu Be Trp Cys Wing Val Gly Asn Cys Tyr Be Leu 500 505 510 Arg Lys Asp His Wing Thr Leu Lys Met Phe Gln Arg Wing Ile Gln 515 520 525 Leu Asn Glu Arg Phe Thr Tyr Wing His Thr Read Cys Gly His Glu Phe 530 535 540 Wing Wing Leu Read Glu Glu Phe Glow Asp Wing Glu Arg Cys Tyr Arg Lys Wing 545 550 555 560 Leu Gly Ile Asp Thr Arg His Tyr Asn Wing Trp Tyr Gly Leu Gly Met 565 570 575 Thr Tyr Leu Arg Gln Glu Lys Phe Glu Phe Ala Gln His Gln Phe Gln 580 585 590 Leu Wing Leu Gln Ile Asn Pro Arg Be Ser Val Ile Met Cys Tyr Tyr

595 600 605 Gly Ile Wing Read His Glu Ser Lys Arg Asn Asp Glu Wing Read Le Met Met 610 615 620 Met Glu Lys Wing Val Leu Thr Asp Wing Lys Asn Pro Read Pro Lys Tyr 625 630 635 640 Tyr Lys Wing His Ile 645 Leu Thr Ser Leu Gly 650 Asp Tyr His Lys Wing 655 Gln Lys Val Leu Glu 660 Glu Leu Lys Glu Cys 665 Wing Pro Gln Glu Ser 670 Ser Val His Wing Ser 675 Leu Gly Lys Ile Tyr 680 Asn Gln Leu Lys Gln 685 Tyr Asp Lys Wing Val 690 Leu His Phe Gly Ile 695 Wing Leu Asp Leu Ser 700 Pro Ser Pro As As Wing Val Lys Ile Lys Wing Tyr Met Glu Arg Leu Ile Leu Pro Asp 705 710 715 720 Glu Leu Val Thr Glu Glu Asn Leu

725

<210> 135

<211> 1835

<212> DNA

<213> Oryza sativa

<220>

<221> misc_feature <222> (729) .. (732) <223> η is a, c, g, or t <400> 135

atggaaaccc taatggtgga ccgcgtccac ggcagcctcc gccgtcttcc tctgcgagcg cctctgcgcc cagttccccg tagcaacttg ctaccttcac aacaaccagc catatgctgc agaagctgcc agagtcccgg tacttgtttg ctatgtcatg gggaagctga agaagccttg tgtcctgtca atgaaccaaa caacagggca ctaccttctt ggagtaattt acaggtacac ctgagcaatt tgtacaagct ctgactcttg atcctcttct tgtgcatact aggtgttgct gaagatgcaa atgaatgttt gtcttcagca ggaactcaca tccacatcaa atgtggaaaa atcggtttct atcttccaat gtgtcagcaa gttttggtga agctgcatgc taacaccact gcagaagtat ctggttatcc tgcatatgca gaacggtgca ccatctaatt tatcacagtt caacgcagnn nnataatgta acttcaactt cgtcttctac atcccgagca agagaaatct gaacgagttc tgtcacagga tcagggagct aatggcactc ttgcggacac taggggaagg ttaagtgtca ggaagcattg gaagtatata gaaagctccc gatgggttct ttgccaggtt gggaagacat attttgaact atcatttttt tgagttagcg catcgactat caccatgcac actccactgt tctttatcat ttgaatgagg aaatgcggct ttgtttctat tgatcgacta tctccccaag catggtgtgc tgaggaaaga tcatgagact gccttgaaga attttcaacg gagttgcata cgctcacacg ctatgcggtc acgatataaa aggtagatga aagacactac aatgcctggt atggccttgg aaaagtttga gtttgctgag catcatttca gaagggcatt ctgttcttat gtgctatctt gggatggcct tgcatgcttt tggaaatgat ggagaaggct atatttgctg ataagaagaa aggctttaat ccttctaggc ctacaaaaat accctgatgc taaaggaaat tgcacctcat gaaagtagta tgtatgcact aacttaacat tcttgacaag gctgtatttt gctttggcat ctgctgctga cgttgctata atacaatctg caatggagaa ttatggatga tgatgatgat gatgatgaga tttaa <210> 136 <211> 757 <212> PRT

<213> Oryza sativa <400> 136

Met Glu Thr Read Met Val Val Asp Arg Val His Gly 1 5 10

Met His Arg Asn Ala Val Phe Leu Cys Glu Arg

gcctcttcat gcaccgcaac 60 ccgagacaaa tgtccagttg 120 ataccacatc ttgaaaggaa 180 cttccgaatg aacctcttac 240 tattgaggtt ccaagtggtg 300 tggcagagtg gaagctgcag 360 atgggcagca tacgaggaat 420 cagtgaagca acagctctac 480 gtcaaacttt gttaatgaaa 540 tagtcctaag caaattaaac 600 tcatgtaaag tcaactgcat 660 tgacactcca tcgccaactt 720 aagtatagtt gatggaagat 780 ctccaaatta gctattggta 840 gtataggctt tcttgcttgt 900 agaggcacaa tttaatactg 960 cgtcaattat ttagaagccg gttggaggga atggacattt 1020 1080 1140 aagttacctt gctcaagatc tgtgggaaat tgctttgcct tgctgtacag cttgactcaa 1200 1260 1320 actataccga tctgcacttc agtggtgtac cttcgccagg ccagataaat ccttgctctt 1380 1440 1500 aaagaggaat gaggaagcct tccactcccc aagtatcaaa tctggatgag ttggaacggc 1560 1620 1680 gatgggaaag atttacaagc tgccctggat ttgaaacctc 1740 1800 1835 agtacacctt ccagatgaac

Ser Leu Arg Leu Phe 15

Read Cys Wing Gln Phe 20 25 30

Pro Glu Val Wing Asn Val Gln Leu Read Leu Thr Thr Cys Tyr Leu His Asn

35 40 45

Asn Gln Pro Tyr Wing Tyr Wing His Ile Leu Lys Gly Lys Lys Leu Pro

50 55 60

Glu Ser Arg Tyr Leu Phe Ala Met Ser Cys Phe Arg Met Asn Leu Leu 65 70 75 80

Glu Arg Glu Wing Glu Wing Leu Cys Pro Val Asn Glu Pro Asn Ile Glu

85 90 95

Val Pro Be Gly Wing Thr Gly His Tyr Leu Read Gly Val Ile Tyr Arg

100 105 110

Tyr Thr Gly Arg Val Glu Wing Wing Glu Wing Gln Phe Val Gln Wing Leu

115 120 125

Thr Leu Asp Pro Leu Leu Trp Wing Tyr Wing Glu Glu Leu Cys Ile Leu

130 135 140

Gly Val Wing Glu Asp Wing Asn Glu Cys Phe Ser Glu Wing Thr Wing Leu 145 150 155 160

Arg Leu Gln Gln Glu Leu Thr Be Thr Be Asn Val Glu Lys Be Asn

165 170 175

Phe Val Asn Glu Asn Arg Phe Read Be Ser Asn Val Ser Ala Be Phe

180 185 190

Gly Asp Be Pro Lys Gln Ile Lys Gln Read His Wing Asn Thr Thr Wing

195 200 205

Glu Val Ser Gly Tyr Pro His Val Lys Ser Thr Wing Read His Met Gln

210 215 220

Asn Gly Ala Pro Be Asn Read Be Gln Phe Asp Thr Pro Be Pro Thr 225 230 235 240

Ser Thr Gln Val Ser Gly Ile Pro Wing Pro Pro Read Phe Arg Asn Met

245 250 255

His Wing Tyr Gln Asn Thr Wing Gly Gly Asn Wing Pro Be Lys Pro Lys

260 265 270

Val Asn Ala Pro Asn Leu Thr Read Le Arg Arg Lys Tyr Ile Asp Glu Wing

275 280 285

Gly Leu Lys Lys Val Ser Gly Arg Leu Phe Asn Gln Ser Asp Ser

290 295 300

Val Pro Arg Arg Be Wing Arg Read Le Arg Asp Thr Thr Ile Asn Ser 305 310 315 320

Asn Be Asn Ile Be Gln Phe Gly Gly Asn Gly Thr Asp His Be Ser

325 330 335

Gly Lys Leu Arg Val Asn Be Ser Thr Thr Be Lys Leu Cys Ser Thr

340 345 350

Wing Leu Arg Be Val Gln Val Arg Lys Gly Lys Pro Gln Wing Thr Glu

355 360 365

Asn Phe Asp Glu Gly Asp Tyr His Phe Asp Met Asp Asp Ser Val Thr

370 375 380

Be Thr Be Be Be Thr Be Ile Val Asp Gly Arg Tyr Pro Glu 385 390 395 400

Glu Lys Be Glu Arg Val Leu Be Gln Asp Be Lys Leu Wing Ile Gly

405 410 415

Ile Arg Glu Leu Met Wing Leu Read Le Arg Thr Read Le Gly Glu Gly Tyr Arg

420 425 430

Leu Ser Cys Leu Phe Lys Cys Gln Glu Wing Leu Glu Val Tyr Arg Lys

435 440 445

Leu Pro Glu Wing Gln Phe Asn Thr Gly Trp Val Leu Cys Gln Val Gly

450 455 460

Lys Thr Tyr Phe Glu Read Val Asn Tyr Read Glu Wing Asp His Phe Phe 465 470 475 480

Glu Leu Wing His Arg Leu Ser Pro Cys Thr Leu Glu Gly Met Asp Ile

485 490 495

Tyr Ser Thr Val Leu Tyr His Leu Asn Glu Glu Met Arg Leu Ser Tyr

500 505 510

Leu Wing Gln Asp Leu Val Val Ile Asp Arg Leu Ser Pro Gln Wing Trp 515 520 525 530

545

550

565

580

595

610

625

630

645

660

675

690

705

710

725

740

Asp Asp Asp Glu Ile

755 <210> 137 <211> 2253 <212> DNA

<213> Solanum tuberosum <4 00> 137

atggaaaccc tactagctga atctgtgcaa aacagccttg gccattttca tgtgtgaacg actctgtgcc gagttcccca ttagctggct gctacctgca caaccaacag gcttatgctg acaagtatgg ctcaatcccg ctacttgttt gcactatcat actgaagctg agacagcact ttgccctcct aatgagccaa gcagctgggc attaccttct tggtcttatt tacaggtata atccagcatt tcaatcaggc attgttattg gatccattgc ttgtgtatac taggtgctgc agaagaagca gctgcagttt tgcattcaga aacaacacat agaccaaggg aaccaatctc gatgatcaga atgtagcttc tacgaatatt gtctcaggcg aaacatacac acagccataa tcttcgagaa atgtctggaa atccagaatt taggtggggt ttctactaac atgtcattct gcatcacagt tgtcaggagt ggttccacct ccagtttgta actaatgcat ctgtggctgg tgctgataat tctccacgag caggcccctc ggagaaagtt tgttgatgag gggaagttaa ttttctgatt ctggccctcg acgaaattca aggcttgctg aattcaaatg tatctggtgc ttctggaaat ggaacaattc agttcgaagc tgagctcaat gactttacgt tccatgacaa gctaccgaaa actatggtga agggactcgc aatgacattt atgactatgt cacacccttc tggagatgct agacctcttg tctgcttctg gggttaatgt aagcagcaca tctatccctc gcccttttca ggtttcttgg ggaaggctat agactttctt gcactggatg tttataacaa actcccacac aaacattatc cagattggaa gagcatactt cgaaatggtt gattacctag cttgctcgtc tggcctcacc ttatagttta gaaggaatgg tttcatctca aggaggacat gaagttggcgc agattagctc gtggctg

Phe Wing Leu Arg Lys Asp His Glu Thr Wing 535 540 Wing Val Gln Leu Asp 555 Ser Arg Val Wing Tyr 560 His Glu Tyr Ser 570 Wing Leu Glu Asp Tyr 575 Glu Arg Ser Wing 585 Leu Gln Val Asp Glu 590 Arg His Leu Gly 600 Val Val Tyr Leu Arg 605 Gln Glu Lys His Phe Arg Arg Wing Phe Gln Ile Asn Pro 615 620 Cys Tyr Leu Gly Met 635 Wing Leu His Wing Leu 640 Leu Glu Met Met 650 Glu Asn Wing Ile Phe 655 Wing Pro Lys Tyr 665 Gln Lys Wing Leu Ile 670 Leu Leu Asp Wing 680 Leu Asp Glu Leu Glu 685 Arg Leu Lys To Be Met Tyr Wing Leu Met Gly Lys Ile 695 700 Leu Asp Lys Wing Val 715 Phe Cys Phe Gly Ile 720 Pro Wing Ile Ile Lys 735 Ser Leu Pro Asp 745 Glu Leu Met Asp Asp 750 Asp Asp

gccaatttat gtaccacaac 60 ctgagacaaa tatgcagctt 120 catatcatct tctcaagggg 180 gctttcagat ggatcttctc 240 ctgcagaggt tccaaatggt 300 cagatagaag aaatagttcc 360 tatgggctgc atatgaggag 420 ttggggaagc atctttgctt 480 aaaatttaca agcatccact 540 acatcagccc tatgcaatca 600 attataatgg agcagctgct 660 acaacactcc ctcaccaatg 720 gaaattttca gcaaaatgga 780 caactgtcaa ttcaaccatt 840 gaaagatatc tgggaggtta 900 gagaatctac tggaaacaca 960 attcttccaa atattatggt 1020 gtcgaaaggc acaatcttgg 1080 ctaatgattc tcggctaaat 1140 aacaagaaag gccccgaact 1200 tcagtgcttc agagatattg 1260 gtttatatag atgtcaggat 1320 acactggatg ggttctttct 1380 aagcagatca tgcatttggc 1440 acgtgtactc gacagtgttg 1500 aggtgctggt atcaactgat 1560 atagtttaca gaaagaccat 1620 atcctagatt tgcatacggg 1680 cacacgcttt gtggtcatga atatgttgct ttagaagatt ttgaaaatgc tattaagagc 1740 tatcagagtg cacttcgtgt ggatgccagg cattacaatg cctggtatgg gcttggaatg 1800 atctatctcc gacaggagaa gtttgaattt tcagagcatc actttcgaat ggctttgggt 1860 ataaatccac agtcttctgt tatcatgtca tatcttggca ctgcattaca cgctctgaag 1920 aaaaatgaag aggcattgga agtgatggag ctggctattg tagcagacaa gaaaaaccct 1980 cttccaatgt atcagaaggc taacatcctt gtgagcacgg aaagttttga tgccgcttta 2040 gaagtcttag aggaacttaa agagcatgct cctcgtgaga gcagtgtcta tgctttgatg 2100 ggtcggatat acaagaggcg taatatgtac gacaaagcca tgcttcattt tggagtggca 2160 ttagatttaa aaccatctgc aactgatgtt gctaccatta aggctgccat tgaaaagctg 2220 catgtaccag atgagatgga agatgaatta cup 2253

<210> 138 <211> 750 <212> PRT

<213> Solanum tuberosum <4 00> 138

Met Glu Thr Read Leu Wing Glu Ser Val Gln Asn Ser Leu Gly Gln Phe 1 5 10 15

Met Tyr His Asn Ala Ile Phe Met Cys Glu Arg Leu Cys Ala Glu Phe

20 25 30

Pro Thr Glu Thr Asn Met Gln Read Leu Wing Gly Cys Tyr Leu His Asn

35 40 45

Gln Gln Wing Tyr Wing Wing Tyr His Read Leu Lys Gly Thr Be Met Wing

50 55 60

Gln Be Arg Tyr Leu Phe Wing Read Be Cys Phe Gln Met Asp Leu Read 65 70 75 80

Thr Glu Wing Glu Thr Wing Leu Cys Pro Asn Glu Pro Thr Glu Wing

85 90 95

Val Pro Asn Gly Wing Gly Wing His Tyr Leu Leu Gly Leu Ile Tyr Arg

100 105 110

Tyr Thr Asp Arg Arg Asn Be Ser Ile Gln His Phe Asn Gln Wing Leu

115 120 125

Leu Leu Asp Pro Leu Leu Trp Wing Tyr Wing Glu Glu Leu Cys Ile Leu

130 135 140

Gly Wing Glu Wing Glu Wing Wing Val Phe Wing Gly Glu Wing Ser Leu Leu 145 150 155 160

Cys Ile Gln Lys Gln His Ile Asp Gln Gly Asn Gln Being Gln Asn Leu

165 170 175

Gln Wing Be Thr Asp Asp Gln Asn Val Wing Be Thr Asn Ile Val Ser

180 185 190

Gly Asp Ile Be Pro Met Gln Be Lys His Thr His Be His Asn Leu

195 200 205

Arg Glu Met Ser Gly Asn Tyr Asn Gly Wing Wing Wing Ile Gln Asn Leu

210 215 220

Gly Gly Val Be Thr Asn Met Be Phe Tyr Asn Thr Pro Be Met 225 230 235 240

Ala Ser Gln Read Ser Gly Val Val Pro Pro Val Val Cys Arg Asn Phe

245 250 255

Gln Gln Asn Gly Thr Asn Wing Ser Val Wing Gly Wing Asp Asn Ser Pro

260 265 270

Arg Wing Thr Val Asn Be Thr Ile Gln Pro Wing Arg Arg Lys Phe Val

275 280 285

Asp Glu Gly Lys Read Arg Lys Ile Be Gly Arg Read Phe Ser Asp Ser

290 295 300

Gly Pro Arg Arg Asn Be Arg Leu Wing Gly Glu Be Thr Gly Asn Thr 305 310 315 320

Asn Be Asn Val Be Gly Wing Be Gly Asn Gly Thr Ile His Be Be

325 330 335

Lys Tyr Tyr Gly Be Be Lys Tyr Be Be Met Thr Read Le Be Arg Be Met

340 345 350

Thr Be Arg Lys Wing Gln Be Trp Wing Thr Glu Asn Tyr Gly Glu Gly

355 360 365

Thr Arg Asn Asp Ile Be Asn Asp Be Arg Read Asn Met Thr Met Be 370 375 380 His Pro Be Gly Asp Wing Arg Pro Read Glu Gln Glu Arg Pro Arg Thr 385 390 395 400 Ser Wing Be Gly Val Asn Val Ser Be Thr Be Ile Pro Leu Sera Wing 405 410 415 Ser Glu Ile Leu Wing Leu Phe Arg Phe Leu Gly Glu Gly Tyr Arg Leu 420 425 430 Ser Cys Leu Tyr Arg Cys Gln Asp Wing Leu Asp Val Tyr Asn Lys Leu 435 440 445 Pro His Lys His Tyr His Thr Gly Trp Val Leu Ser Gln Ile Gly Arg 450 455 460 Wing Tyr Phe Glu Met Val Asp Tyr 485 490 495 Ser Thr Val Leu Phe His Leu Lys Glu Asp Met Lys Leu Ser Tyr Leu 500 505 510 Wing Gln Val Leu Val Ser Thr Asp Arg Leu Pro Gln Ser Trp Cys 515 520 525 Wing Met Gly Asn Cys Tyr Be Leu Gln Lys Asp His Glu Thr Wing Leu 530 535 540 Lys Asn Phe Gln Arg Wing Val Gln Leu Asn Pro Arg Phe Wing Tyr Gly 545 550 555 560 His Thr Leu Cys Gly His Glu Tyr Val Wing Leu Glu Asp Phe Glu Asn 565 570 575 Wing Ile Lys Ser Tyr Gln Be Wing Leu Arg Val Asp Wing Ala Arg His Tyr 580 585 590 Asn Wing Trp Tyr Gly Leu Gly Met Ile Tyr Leu Arg Gln Glu Lys Phe 595 600 605 Glu Phe Ser Glu His His Phe Arg Met Wing Leu Gly Ile Asn Pro Gln 610 615 620 Ser Ser Val Ile Met Ser Tyr Leu Gly Thr Wing Read His His Wing Read Lys 625 630 635 640 Lys Asn Glu Glu Wing Read Leu Glu Val Met Glu Leu Wing Ile Val Wing Asp 645 650 655 Lys Lys Asn Pro Leu Pro Met Tyr Gln Lys Wing Asn Ile Leu Val Ser 660 665 670 Thr Ser Phe Asp Wing Ala Leu Glu Val Leu Glu Glu Leu Lys Glu 675 680 685 His Wing Pro Arg Glu Being Ser Val Tyr Wing Leu Met Gly Arg Ile Tyr 690 695 700 Lys Arg Arg Asn Met Tyr Asp Lys Wing Met His Le Phe Gly Val Wing 705 710 715 720 Leu Asp Leu Lys Pro Be Wing Thr Asp Val Wing Thr Ile Lys Wing Wing 725 730 735 Ile Glu Lys Read His Val Pro Asp Glu Met

<212> DNA

<213> Schizosaccharomyces pombe

<400> 139

atgacagatc gattgaaatg tttaatatgg tattgcattg ataatcagaa ttatgataat 60

tcaatttttt attcagaacg tttacatgca attgaagatt caaacgagag tttgtatctt 120

ttggcatatt cgcatttcct aaacctcgat tacaatattg tatacgactt attagataga 180

gtaattagtc atgttccttg cacatactta tttgcaagga ccagccttat tttaggcaga 240

tataaacaag gaataagtgc tgtggaggcc tgtcgatcga attggcgctc cattcagcca 300

aacataaatg actcaattag cagtcgtgga catccagatg cctcttgcat gcttgatgtt 360

ttgggtacta tgtataaaaa ggcagggttc ctcaaaaaag ctacagattg ttttgtagaa 420

gctgtctcca ttaacccata taatttctct gctttccaga atttaactgc aataggcgtg 480

ccactcgatg ctaataatgt atttgttatt ccaccctacc ttacggcaat gaagggtttt 540

gaaaaatctc aaacgaatgc tacagcttcg gtaccagaac cgtctttttt gaagaaaagt 600

aaagagtctt cctcatcttc caacaagttt tcggtttctg aatcgatagc aaatagttat 660

tcaaactcat ccatttcagc atttactaag tggtttgata gggttgacgc ttctgagctt 720 ccaggaagtg agaaggaacg acatcaaagc ttgaaattac aatctcaatc tcagactagc 780

aaaaaccttt tggctttcaa tgatgctcaa aaagctgatt ctaacaatag ggatacgtct 840

ttaaaatccc actttgtgga acctagaacc caagcattaa gaccaggagc tcgtttaaca 900

tataaattac gcgaagcgag aagttctaaa agaggagaga gcacacctca aagcttccgc 960

gaagaggaca ataatttgat ggaattacta aagttattcg gtaagggtgt ttacctgctc 1020

gcccagtata agttacgaga ggctttaaat tgtttccaaa gcttgcccat cgaacagcaa 1080

aatacacctt ttgttcttgc aaagcttgga ataacctact ttgaactggt tgattacgaa 1140

aaatctgaag aagtgtttca aaaattaagg gacttgtcgc cttcacgtgt caaagatatg 1200

gaagtctttt caactgcact ttggcatttg caaaagtctg ttcctttatc ttaccttgcc 1260

catgaaactt tggaaactaa tccttattcc ccagaatcat ggtgcattct tgctaattgc 1320

ttctcacttc aacgtgaaca ctcgcaggca ttaaaatgta ttaatagagc tattcaattg 1380

gatccaactt ttgaatatgc ttatacgctt caagggcacg agcattctgc aaacgaagaa 1440

tacgaaaaat cgaaaacatc tttccgcaaa gcaattagag taaatgttcg acattacaat 1500

gcatggtatg gcctgggaat ggtttattta aaaacagggc gaaatgatca agcagacttt 1560

cattttcaac gtgctgcaga gatcaatcct aacaactctg tactcatcac ttgtattggt 1620

atgatttacg aacgctgcaa agattacaaa aaagcacttg atttttatga tcgggcatgt 1680

aaactggatg aaaagtcttc gcttgccagg ttcaagaaag ccaaagtgct tattttatta 1740

catgatcacg ataaagcact cgttgaattg gaacaattaa aggcaattgc gccagatgaa 1800

gcaaatgttc attttttgct cggcaaaatt ttcaagcaaa tgcggaaaaa aaatttagcc 1860

ttaaagcact tcactatagc atggaactta gacggcaagg ctacgcatat tattaaggaa 1920

tcgattgaaa atctggatat tcccgaagaa aatttgttaa ctgaaacagg tgaaatttat 1980

aggaatctgg aaacttaa 1998 <210> 140 <211> 665 <212> PRT

<213> Schizosaccharomyces pombe <400> 140

Met Thr Asp Arg Leu Lys Cys Leu Ile Trp Tyr Cys Ile Asp Asn Gln 1 5 10 15 Asn Tyr Asp Asn Ser Ile Phe Tyr Ser Glu Arg Leu His Ala Ile Glu 20 25 30 Asp Ser Asn Glu Ser Leu Tyr Leu Alu Tyr Be His Phe Read Asn 35 40 45 Read Asp Tyr Asn Ile Val Tyr Asp Read Leu Asp Arg Val Ile Be His 50 55 60 Val Pro Cys Thr Tyr Leu Phe Ala Thr Thr Be Read Ile Leu Gly Arg 65 70 75 80 Tyr Lys Gln Gly Ile Be Ala Val Glu Wing Cys Arg Be Asn Trp Arg 85 90 95 Be Ile Gln Pro Asn Ile Asn Asp Be Ile Be Be Arg Gly His Pro 100 105 110 Asp Wing Be Cys Met Leu Asp Val Leu Wing 115 120 125 Gly Phe Leu Lys Lys Wing Thr Asp Cys Phe Val Glu Wing Val Ser Ile 130 135 140 Asn Pro Tyr Asn Phe Be Wing Phe Gln Asn Leu Thr Wing Ile Gly Val 145 150 155 160 Pro Leu Asp Wing Asn Asn Val Phe Val Ile Pro Tyr Leu Thr Wing 165 170 175 Met Lys Gly r Asn Wing Thr Thr Wing Be Val Pro 180 185 190 Glu Pro Be Phe Leu Lys Lys Be Lys Glu Be Ser Be Ser Be Asn 195 200 205 Lys Phe Ser Val Be Glu Ser Ile Be Ala Phe Thr Lys Trp Phe Asp Arg Val Asp Ala Be Glu Leu 225 230 235 240 Pro Gly Be Glu Lys Glu Arg His Gln Gln Wing Lys Wing 260 265 270 Asp Be Asn Asn Arg Asp Thr Be Leu Lys Be His Phe Val Glu Pro 275 280 285 Arg Thr Gln Wing Leu Arg Pro Gly Wing Arg Leu Thr Tyr Lys Leu Arg

290 295 300

Glu Wing Arg Be Be Lys Arg Gly Glu Be Thr Pro Gln Be Phe Arg 305 310 315 320

Glu Glu Asp Asn Asn Leu Met Glu Leu Leu Lys Leu Phe Gly Lys Gly

325 330 335

Val Tyr Leu Leu Wing Gln Tyr Lys Leu Arg Glu Wing Leu Asn Cys Phe

340 345 350

Gln Be Leu Pro Ile Glu Gln Gln Asn Thr Pro Phe Val Leu Wing Lys

355 360 365

Read Gly Ile Thr Tyr Phe Glu Read Val Asp Tyr Glu Lys Be Glu Glu

370 375 380

Val Phe Gln Lys Read Arg Asp Read Be Pro Be Arg Val Lys Asp Met 385 390 395 400

Glu Val Phe Ser Thr Wing Leu Trp His Leu Gln Lys Ser Val Pro Leu

405 410 415

Ser Tyr Leu Wing His Glu Thr Leu Glu Thr Asn Pro Tyr Ser Pro Glu

420 425 430

Ser Trp Cys Ile Leu Wing Asn Cys Phe Ser Leu Gln Arg Glu His Ser

435 440 445

Gln Wing Read Lys Cys Ile Asn Arg Wing Ile Gln Read Asp Pro Thr Phe

450 455 460

Glu Tyr Wing Tyr Thr Read Gln Gly His Glu His Ser Wing Asn Glu Glu 465 470 475 480

Tyr Glu Lys Be Lys Thr Be Phe Arg Lys Wing Ile Arg Val Asn Val

485 490 495

Arg His Tyr Asn Wing Trp Tyr Gly Leu Gly Met Val Tyr Leu Lys Thr

500 505 510

Gly Arg Asn Asp Gln Wing Asp Phe His Phe Gln Arg Wing Wing Glu Ile

515 520 525

Asn Pro Asn Asn Be Val Leu Ile Thr Cys Ile Gly Met Ile Tyr Glu

530 535 540

Arg Cys Lys Asp Tyr Lys Lys Wing Read Asp Phe Tyr Asp Arg Cys Wing 545 550 555 560

Lys Leu Asp Glu Lys Being Ser Leu Wing Arg Phe Lys Lys Wing Lys Val

565 570 575

Leu Ile Leu Leu His Asp His Asp Lys Wing Leu Val Glu Leu Glu Gln

580 585 590

Leu Lys Wing Ile Wing Pro Asp Glu Wing Asn Val His Phe Leu Leu Gly

595 600 605

Lys Ile Phe Lys Gln Met Arg Lys Lys Asn Leu Wing Leu Lys His Phe

610 615 620

Thr Ile Wing Trp Asn Leu Asp Gly Lys Wing Thr His Ile Ile Lys Glu 625 630 635 640

Ser Ile Asu Leu Asu Leu Asp Ile Pro Glu Asn Leu Asu Leu Thr Glu Thr

645 650 655

Gly Glu Ile Tyr Arg Asn Read Glu Thr 660 665

<210> 141 <211> 2421 <212> DNA

<213> Aspergillus niger <4 00> 141

atgactccgt ccacatcaca tatttcgagc cagctaaggc agctgatata ttaccatctt 60

gacaacaacc ttgctcggaa cgcgctgttc cttgccggtc gtttacacgc ctacgaacct 120 cggacgtcgg aagcttcgta cctattagct ctgtgttacc tacaaaatgg tcaggtgaaa 180 gcagcatggg aaactagcaa gcattttggg tcgaggggtg cgcatcttgg atgttcttac 240 gtctacgcgc aggcttgtct tgacctcggg aaatatacgg acggtattaa cgcgctagag 300 cgaagtaagg gacaatggac ttcgcgaaac cactggaata aacacagtga gacgcgacga 360 caacacttgc cggatgctgc ggcagtttta tgtttgcaag gaaaattatg gcaggcacac 420 aaggaacaca acaaggctgt ggagtgttac gctgcagctt taaagctgaa tcccttcatg 480 tgggatgcat tcttgaatct gtgcgaaact ggtgttgatt tgcgtgtttc aaacatatat 540 aagatgagcc cggaattgta cagcatggta tcgtctgctg cgctcgaaga tgttgaatcc 600 caggttctac ctccggacgggccacggccgcctcc 6cc

ccgttcactg ccggtacgac tcgcagcgat tcgacctcta ctcacgggag ctcagctttg 720

tgggaaaagt tgaatggaag cacagttagt gtggcatctt caggaacagg accgcatctt 780

ccgcgggaag gcatggagac tcctggtggc caaagtagcg aatctgatga tccacgtgta 840

accaacggta atggcacaga tgtttttgag ccgccgctag cccctgcaaa gaagaatcga 900

acgatccaaa caataggcgg cgatcatccg atggatcccc cgccaaagat gcggccaacc 960

ggtattcgac cgaggacccg aaccaaattc gagtcagatg aaggtcatac tgagagagac 1020

gcgggcatgg gtcacaggtt aggcgatcgg aaacgaacgg tctctggaca agttgcgcat 1080

ccgtcggtac cgcattcaac cgaccaaggt gtcgggcaac ggcgaagtgt acgtctcttt 1140

aaccagataa agccatcaac gaacaaaata tctagcacgg cattaggagt gaaagagggc 1200

cgggaggtga aaaaggtgag aactacgggg aataaggcgc gcacaacaac aagctcaaat 1260

gtgggccgtg ttgttagcgg caacaatcga cgacacgctg gggagatcca tgacggagac 1320

agcaaagaat accgtggaac atcttcaaca tcaaatggct ctcagaacgc atcgtctaag 1380

ctcgctatat ctgagcggac caaatcggtt gaagccttgg cttggatttt ggacttgttt 1440

ttcaagatag cctccggcta tttctgtctc agccggtaca aatgctccga tgctatccag 1500

attttcagct ctctatccca gggccaacgg gagacaccgt gggttcttgc tcaaattgga 1560

cgagcttact acgagcaagc aatgtataca gaggccgaga aatactttgt ccgggtgaag 1620

gccatggcgc cttcccggtt agaagatatg gagatctact cgacggttct ttggcatttg 1680

aagaatgatg tcgaacttgc ttacctggcc catgagttaa tggatgtcga tcgcttatca 1740

ccagaagctt ggtgtgctgt cggtaactcg ttctcacacc agcgggacca cgatcaagct 1800

ctgaagtgct tcaagcgtgc cactcaattg gatcctcatt tcgcgtatgg gttcacgcta 1860

cagggccatg agtatgttgc caacgaggaa tatgacaagg cattggatgc ctatagaagc 1920

ggcatcaacg cggacagtcg acattataat gcctggtacg ggctgggaac ggtctacgat 1980

aagatgggca aacttgactt tgctgaacag cacttccgta atgcggccaa gattaatcct 2040

tctaatgctg ttttaatatg ctgcattggc ttggtcttag agaagatgaa caatccgaag 2100

tcagcgttga tccagtacaa cagggcctgc accctcgcgc ctcattctgt tcttgctcga 2160

ttccgaaaag cccgtgcgtt gatgaaattg caggatctca agtcagcgct tacggagctg 2220

aaggttctga aggacatggc gccggatgag gcaaacgttc attacctcct gggcaaactg 2280

tataagatgc ttcgtgacaa gggaaatgcc attaagcact tcacaactgc attaaacctt 2340

gatcccaagg cggcgcagta catcaaagat gcaatggaag cccttgacga cgatgaagag 2400

gatgaagaag atatggcgtg at 2421 <210> 142 <211> 806 <212> PRT

<213> Aspergillus niger <4 00> 142

Met Thr Pro Be Thr Be His Ile Be Be Gln Leu Arg Gln Leu Ile 1 5 10 15 Tyr Tyr His Leu Asp Asn Asn Leu Arg Wing Asn Leu Phe Leu Wing 20 25 30 Gly Arg Leu His Wing Tyr Glu Pro Arg Thr Glu Wing Ser Tyr Leu 35 40 45 Leu Wing Leu Cys Tyr Leu Gln Asn Gly Gln Val Lys Wing Trp Glu 50 55 60 Thr Be Lys His Phe Gly Be Arg Gly Wing His Leu Gly Cys Ser Tyr 65 70 75 80 Val Tyr Wing Gln Ala Cys Leu Asp Leu Gly Lys Tyr Thr Asp Gly Ile 85 90 95 Asn Ala Leu Glu Arg Be Lys Gly Gln Trp Thr Be Arg Asn His Trp 100 105 110 Asn Lys His Be Glu Thr Arg Arg Wing 115 120 125 Val Leu Cys Leu Gln Gly Lys Leu Trp Gln Wing His Lys Glu His Asn 130 135 140 Lys Wing Val Glu Cys Tyr Wing Wing Leu Lys Leu Asn Pro Phe Met 145 150 155 160 Trp Asp Wing Phe Leu Asn Leu Cys Glu Thr Gly Val Asp Leu Arg Val 165 170 175 Ser Asn Ile Tyr Lys Met Ser G lu Leu Tyr Ser Met Val Ser Ser 180 185 190 Wing Wing Leu Glu Asp Val Glu Ser Gln Val Leu Pro Pro Asp Gly Pro 195 200 205 Leu Gln Thr Gln Val Asn Pro Asn Pro Seru Asp Pro Phe Thr Wing 210 215 220

Gly Thr Thr Arg Be Asp Be Thr Be His Thr Gly Be Ser Wing Leu 225 230 235 240 Trp Glu Lys Leu Asn Gly Thr Thr Val Be Val Wing Be Gly Thr 245 250 255 Gly Pro His Leu Pro Glu Gly Met Glu Thr Pro Gly Gly Gln Be 260 265 270 Be Glu Be Asp Asp Pro Arg Val Thr Asn Gly Asn Gly Thr Asp Val 275 280 285 Phe Glu Pro Pro Read Wing Ala Pro Lys Lys Asn Arg Thr Ile Gln Thr 290 295 300 His Pro Met Asp Pro Pro Pro Lys Met Arg Pro Thr 305 310 315 320 Gly Ile Arg Pro Arg Thr Arg Lys Phe Glu Being Asp Glu Gly His 325 330 335 Thr Glu Arg Asp Wing Gly Met Gly His Arg Leu Gly Asp Arg Lys Arg 340 345 350 Thr Val Be Gly Gln Val Wing His Pro Be Val Pro His Be Thr Asp 355 360 365 Gln Gly Val Gly Gln Arg Arg Be Val Arg Leu Phe Asn Gln Ile Lys 370 375 380 Pro Be Thr Asn Lys Ile Be Thr Wing Leu Gly Val Lys Glu Gly 385 390 395 400 Arg Glu Val Lys Lys Val Arg Thr Thr Gly Asn Lys Wing Arg Thr Thr 405 410 415 Thr Be Ser Asn Val Gly Arg Val Val Be Gly Asn Arg Arg His 420 425 430 Wing Gly Glu Ile His Asp Gly Asp Be Lys Glu Tyr Arg Gly Thr Ser 435 440 445 Ser Thr Be Asn Gly Ser Gln Asn Wing Ser Be Lys Leu Wing Ile Ser 450 455 460 Glu Arg Thr Lys Ser Val Glu Wing Leu Wing Trp Ile Leu Asp Leu Phe 465 470 475 480 Phe Lys Ile Wing Ser Gly Tyr Phe Cys Leu To Be Arg Tyr Lys Cys To 485 490 495 Asp Ala Ile Gln Ile Phe To Be Leu To Be Gln Gly Gln Arg Glu Thr 500 505 510 Pro Trp Val Leu Ala Gln Ile Gly Arg Wing Tyr Tyr Glu Gln Ala Met 515 520 525 Tyr Thr Glu Wing Glu Lys Tyr Phe Val Arg Val Lys Wing Met Pro Wing 530 535 540 Ser Arg Leu Glu Asp Met Glu Ile Glu Read Met Asp Val 565 570 575 Asp Arg Read Le Pro Glu Wing Trp Cys Ala Val Gly Asn Ser Phe Ser 580 585 590 His Gln Arg Asp His Asp Gln Ala Leu Read Lys Cys Phe Lys Arg Ala Thr 595 600 605 Gln Read Asp Pro His Phe Ala Tyr Gly Phe Thr Tyr Val Wing Asn Glu Glu Tyr Asp Lys Wing Leu Asp Wing Tyr Arg Ser 625 630 635 640 Gly Ile Asn Wing Asp Being Arg His Tyr Asn Wing Trp Tyr Gly Leu Gly 645 650 655 Thr Val Tyr Asp Lys Met Gly Lys Leu Asp Phe Glu Wing Gln His Phe 660 665 670 Arg Asn Wing Wing Lys Ile Wing Asn Pro Ser Asn Wing Val Leu Wing Ile Cys 675 680 685 Ile Gly Leu Val Leu Glu Met Wing Asn Pro Lys Wing Wing Ile 690 695 700 Gln Tyr Asn Arg Wing Cys Thr Leu Pro Wing His Be Val Leu Wing Arg 705 710 715 720 Phe Arg Lys Wing Arg Wing Wing Met Lys Leu Gln Asp Leu Lys Ser Wing

725 730 735

Leu Thr Glu Leu Lys Val Leu Lys Asp Met Wing Pro Asp Glu Wing Asn

740 745 750

Val His Tyr Leu Read Gly Lys Leu Tyr Lys Met Leu Arg Asp Lys Gly

755 760 765

Asn Ala Ile Lys His Phe Thr Thr Wing Read Asn Leu Asp Pro Lys Wing

770 775 780

Gln Wing Tyr Ile Lys Asp Wing Met Glu Wing Read Le Asp Asp Asp Glu Glu 785 790 795 800

Asp Glu Glu Asp Met Wing 805

<210> 143

<211> 2472

<212> DNA

<213> Homo sapiens

<4 00> 143

atgacggtgc tgcaggaacc cgtccaggct gctatatggc aagcactaaa ccactatgct 60

taccgagatg cggttttcct cgcagaacgc ctttatgcag aagtacactc agaagaagcc 120

ttgtttttac tggcaacctg ttattaccgc tcaggaaagg catataaagc atatagactc 180

ttgaaaggac acagttgtac tacaccgcaa tgcaaatacc tgcttgcaaa atgttgtgtt 240

gatctcagca agcttgcaga aggggaacaa atcttatctg gtggagtgtt taataagcag 300

aaaagccatg atgatattgt tactgagttt ggtgattcag cttgctttac tctttcattg 360

ttgggacatg tatattgcaa gacagatcgg cttgccaaag gatcagaatg ttaccaaaag 420

agccttagtt taaatccttt cctctggtct ccctttgaat cattatgtga aataggtgaa 480

aagccagatc ctgaccaaac atttaaattc acatctttac agaactttag caactgtctg 540

cccaactctt gcacaacaca agtacctaat catagtttat ctcacagaca gcctgagaca 600

gttcttacgg aaacacccca ggacacaatt gaattaaaca gattgaattt agaatcttcc 660

aattcaaagt actccttgaa tacagattcc tcagtgtctt atattgattc agctgtaatt 720

tcacctgata ctgtcccact gggaacagga acttccatat tatctaaaca ggttcaaaat 780

aaaccaaaaa ctggtcgaag tttattagga ggaccagcag ctcttagtcc attaacccca 840

agttttggga ttttgccatt agaaacccca agtcctggag atggatccta tttacaaaac 900

tacactaata cacctcctgt aattgatgtg ccatccaccg gagccccttc aaaaaagtct 960

gttgccagaa tcggccaaac tggaacaaag tctgtcttct cacagagtgg aaatagccga 1020

gaggtaactc caattcttgc acaaacacaa agttctggtc cacaaacaag tacaacacct 1080

caggtattga gccccactat tacatctccc ccaaacgcac tacctcgaag aagttcacga 1140

ctctttacta gtgacagctc cacaaccaag gagaatagca aaaaattaaa aatgaagttt 1200

ccacctaaaa tcccaaacag aaaaacaaaa agtaaaacta ataaaggagg aataactcaa 1260

cctaacataa atgatagcct ggaaattaca aaattggact cttccatcat ttcagaaggg 1320

aaaatatcca caatcacacc tcagattcag gcctttaatc tacaaaaagc agcagcaggt 1380

ttgatgagcc ttcttcgtga aatggggaaa ggttatttag ctttgtgttc atacaactgc 1440

aaagaagcta taaatatttt gagccatcta ccttctcacc actacaatac tggttgggta 1500

ctgtgccaaa ttggaagggc ctattttgaa ctttcagagt acatgcaagc tgaaagaata 1560

ttctcagagg ttagaaggat tgagaattat agagttgaag gcatggagat ctactctaca 1620

acactttggc atcttcaaaa agatgttgct ctttcagttc tgtcaaaaga cttaacagac 1680

atggataaaa attcgccaga ggcctggtgt gctgcaggga actgtttcag tctgcaacgg 1740

gaacatgata ttgcaattaa attcttccag agagctatcc aagttgatcc aaattacgct 1800

tatgcctata ctctattagg gcatgagttt gtcttaactg aagaattgga caaagcatta 1860

gcttgttttc gaaatgctat cagagtcaat cctagacatt ataatgcatg gtatggttta 1920

ggaatgattt attacaagca agaaaaattc agccttgcag aaatgcattt ccaaaaagcg 1980

cttgatatca accctcaaag ttcagtttta ctttgccaca ttggagtagt tcaacatgca 2040

ctgaaaaaat cagagaaggc tttggatacc ctaaacaaag ccattgtcat tgatcccaag 2100

aaccctctat gcaaatttca cagagcctca gttttatttc gaaatgaaaa atataagtct 2160

gctttacaag aacttgaaga attgaaacaa attgttccca aagaatccct cgtttacttc 2220

ttaataggaa aggtttacaa gaagttaggt caaacgcacc tcgccctgat gaatttctct 2280

tgggctatgg atttagatcc taaaggagcc aataaccaga ttaaagaggc aattgataag 2340

cgttatcttc cagatgatga ggagccaata acccaagaag aacagatcat gggaacagat 2400

gaatcccagg agagcagcat gacagatgcg gatgacacac aacttcatgc agctgaaagt 2460

gatgaatttt aa 2472 <210> 144 <211> 824 <212> PRT

<213> Homo sapiens <400> 144 Met Thr Val Leu 1

Asn His Tyr Wing 20

Glu Val His Wing 35

Tyr Arg Ser Gly 50

Ser Cys Thr Thr 65

Asp Read Ser Lys

Phe Asn Lys Gln 100

Ser Ala Cys Phe 115

Asp Arg Leu Wing 130

Asn Pro Phe Leu 145

Lys Pro Asp Pro

Ser Asn Cys Leu 180

Read Ser His Arg 195

Thr Ile Glu Leu 210

Ser Leu Asn Thr 225

Be Pro Asp Thr

Gln Val Gln Asn 260

Wing Wing Read Ser 275

Thr Pro Ser Pro 290

Pro Pro Val Ile 305

Val Wing Arg Ile

Gly Asn Ser Arg 340

Gly Pro Gln Thr 355

Ser Pro Pro Asn 370

Asp Ser Ser Thr 385

Pro Pro Lys Ile

Gly Ile Thr Gln 420

Asp Ser Ser Ile 435

Ile Gln Wing Phe 450

Leu Leu Arg Glu 465

Cys Lys Glu Wing

Gln Glu Pro Val 5

Tyr Arg Asp Wing

Be Glu Glu Wing 40

Lys Wing Tyr Lys 55

Pro Gln Cys Lys 70

Read Wing Glu Gly 85

Lys Ser His Asp

Thr Leu Ser Leu 120

Lys Gly Ser Glu 135

Trp Ser Pro Phe 150

Asp Gln Thr Phe 165

Pro Asn Ser Cys

Gln Pro Glu Thr 200

Asn Arg Read Asn 215

Asp Ser Ser Val 230

Val Pro Leu Gly 245

Lys Pro Lys Thr

Pro Leu Thr Pro 280

Gly Asp Gly Ser 295

Asp Val Pro Ser 310

Gly Gln Thr Gly 325

Glu Val Thr Pro

Ser Thr Thr Pro 360

Wing Read Pro Arg 375

Thr Lys Glu Asn 390

Pro Asn Arg Lys 405

Pro Asn Ile Asn

Ile Ser Glu Gly 440

Asn Leu Gln Lys 455

Met Gly Lys Gly 470

Ile Asn Ile Leu 485

Gln Wing Wing Ile 10

Val Phe Leu Wing 25

Leu Phe Leu Leu

Wing Tyr Arg Leu 60

Tyr Leu Leu Wing 75

Glu Gln Ile Leu 90

Asp Ile Val Thr 105

Read Gly His Val

Cys Tyr Gln Lys 140

Glu Ser Leu Cys 155

Lys Phe Thr Ser 170

Thr Thr Gln Val 185

Val Leu Thr Glu

Read Glu Ser Ser 220

Ser Tyr Ile Asp 235

Thr Gly Thr Ser 250

Gly Arg Ser Leu 265

To be Phe Gly Ile

Tyr Leu Gln Asn 300

Thr Gly Wing Pro 315

Thr Lys Ser Val 330

Ile Leu Wing Gln 345

Gln Val Leu Ser

Arg Ser Ser Arg 380

Ser Lys Lys Leu 395

Thr Lys Ser Lys 410

Asp Ser Leu Glu 425

Lys Ile Ser Thr

Wing Wing Wing Wing 460

Tyr Leu Wing Leu 475

Be His Leu Pro 490

Trp Gln Wing Leu 15 Glu Arg Leu Tyr 30 Wing Thr Cys Tyr 45 Leu Lys Gly His Lys Cys Val 80 Ser Gly Gly Val 95 Glu Phe Gly Asp 110 Tyr Cys Lys Thr 125 Ser Leu Ser Leu Glu Ile Gly Glu 160 Leu Gln Asn Phe 175 Pro Asn His Ser 190 Thr Pro Gln Asp 205 Asn Ser Lys Tyr Ser Ala Val Ile 240 Ile Read Ser Lys 255 Leu Gly Gly Pro 270 Leu Pro Leu Glu 285 Tyr Thr Asn Thr Ser Lys Lys Ser 320 Phe Ser Gln Ser 335 Thr Gln Ser Ser 350 Pro Thr Ile Thr 365 Leu Phe Thr Ser Lys Met Lys Phe 400 Thr Asn Lys Gly 415 Ile Thr Lys Leu 430 Ile Thr Pro Gln 445 Gly Leu Met Ser Cys Ser Tyr Asn 480 Ser His His Tyr

495 Asn Thr Gly Trp Val Leu Cys Gln Ile Gly Arg Wing Tyr Phe Glu Leu 500 505 510 Ser Glu Tyr Met Gln Wing Glu Arg Ile Phe Ser Glu Val Arg Arg Ile 515 520 525 Glu Asn Tyr Arg Val Glu Gly Met Glu Ile Tyr Ser Thr Thr Leu Trp 530 535 540 His Leu Gln Lys Asp Val Ala Leu Ser Val Leu Ser Lys Asp Leu Thr 545 550 555 560 Asp Met Asp Lys Asn Ser Pro Glu Ala Trp Cys Ala Wing Gly Asn Cys 565 570 575 Phe Ser Leu Gln Arg Glu His Asp Ile Ala Ile Lys Phe Phe Gln Arg 580 585 590 Ala Ile Gln Val Asp Pro Asn Tyr Ala Tyr Tyr Thr Leu Leu Gly 595 600 605 His Glu Phe Val Leu Thr Glu Leu Asp Lys Ala Leu Cys Wing Phe 610 615 620 Arg Asn Ala Ile Arg Val Asn Pro Arg His Tyr Asn Ala Trp Tyr Gly 625 630 635 640 Leu Gly Met Ile Tyr Lyr Glu Lys Phe Ser Leu Ala Glu Met 645 650 655 His Phe Gln Lys Ala Leu Asp Ile Asn Pro Gln Ser Ser Val Leu 660 665 670 Cys His Ile Gly Val Val Gln His Wing Leu Lys Lys Ser Glu Lys Wing 675 680 685 Leu Asp Thr Leu Asn Lys Wing Ile Val Ile Asp Pro Lys Asn Pro Leu 690 695 700 Cys Lys Phe His Arg Wing Ser Val Leu Phe Asn Glu Lys Tyr Lys 705 710 715 720 Ser Wing Leu Gln Glu Leu Glu Glu Leu Lys Gln Ile Val Pro Lys Glu 725 730 735 Ser Leu Val Tyr Phe Leu Ile Gly Lys Val Tyr Lys Lys Leu Gly Gln 740 745 750 Thr His Leu Ala Leu Met Asn Phe Ser Trp Wing Met Asp Leu Asp Pro 755 760 765 Lys Gly Wing Ace η Asn Gln Ile Lys Glu Wing Ile Asp Lys Arg Tyr Leu 770 775 780 Pro Asp Asp Glu Glu Pro Ile Thr Gln Glu Gln Ile Met Gly Thr 785 790 795 800 Asp Glu Being Gln Glu Being Met Thr Asp Wing Asp Asp Thr Gln Leu 805 810 815 His Wing Glu Wing Being Asp Glu Phe 820 <210> 145

<211> 783 <212> DNA

<213> Saccharum officinarum <220>

<221> misc_feature <222> (780) .. (783) <223> η is a, c, g, or t <400> 145

atggaaaccc taatggtgga ccgcgtccac agcagcctcc gcctcttcat gcaccgcaac 60

gccgtattcc tctgcgagcg cctctgcgcg cagttcccct ccgagaccaa tgtgcaattg 120

ttagcgacct gctacctcca caacaatcag ccatatgctg cataccacat tttgaaaggg 180

aagaagctgc cggagtcccg gtacttgttt gctacatcat gctttcgaat gaacctcttg 240

cgtgaagcag aagaaactct atgtccagtc aatgaaccaa acatggaggt tccaagtgga 300

gcaacaggac actacctcct tggagtgatt tacaggtgca caggcagaat ttcagctgca 360

gctgaacaat ttacacaagc gttgactcta gatcctcttt tatgggcggc atatgaggaa 420

ttgtgtatat taggtattgc tgaagatact gatgagtgtt ttagtgaatc gactgctcta 480

cgtctccagc aggaacacac atccacggcc actctggtga agtcgaactt cgccaatgaa 540

aatcgagttc tatcatccag ggtctctgca aatcttgggg atattagtcc taagcaaatc 600

aaacagcttc atgctaacaa catagcagaa gtatctggct atcctcatgt aagaccaact 660

gcattgcatg tgcagaacag ttcaacctct aatgtagcac agtttgacac cccatcacca 720 actgcagcac agacttctag tatcatgcca ccaccactct ttaggaatgt ccatgcttan 780 nnn 7 83

<210> 146 <211> 259 <212> PRT

<213> Saccharum officinarum <4 00> 146

Met Glu Thr Leu Met Val Asp Arg Val His Being Ser Leu Arg Leu Phe 1 5 10 15

Met His Arg Asn Ala Val Phe Leu Cys Glu Arg Leu Cys Ala Gln Phe

20 25 30

To Be Glu Thr Asn Val Gln Read Leu Wing Thr Cys Tyr Leu His Asn

35 40 45

Asn Gln Pro Tyr Wing Tyr Wing His Ile Leu Lys Gly Lys Lys Leu Pro

50 55 60

Glu Ser Arg Tyr Leu Phe Ala Thr Be Cys Phe Arg Met Asn Leu Leu 65 70 75 80

Arg Glu Wing Glu Glu Thr Read Cys Pro Val Asn Glu Pro Asn Met Glu

85 90 95

Val Pro Be Gly Wing Thr Gly His Tyr Leu Read Gly Val Ile Tyr Arg

100 105 110

Cys Thr Gly Arg Ile Being Wing Wing Wing Glu Gln Phe Thr Gln Wing Leu

115 120 125

Thr Leu Asp Pro Leu Leu Trp Wing Tyr Wing Glu Glu Leu Cys Ile Leu

130 135 140

Gly Ile Glu Wing Asp Thr Asp Glu Cys Phe Be Glu Be Thr Wing Leu 145 150 155 160

Arg Read Gln Gln Glu His Thr Be Thr Wing Thr Read Le Val Lys Ser Asn

165 170 175

Phe Ala Asn Glu Asn Arg Val Leu Be Ser Arg Val Be Ala Asn Leu

180 185 190

Gly Asp Ile Be Pro Lys Gln Ile Lys Gln Read His Wing Asn Asn Ile

195 200 205

Glu Val Wing Ser Gly Tyr Pro His Val Arg Pro Thr Wing Leu His Val

210 215 220

Gln Asn Be Thr Be Asn Val Wing Gln Phe Asp Thr Be Pro 225 230 235 240

Thr Wing Wing Gln Thr Be Ser Ile Met Pro Pro Leu Phe Arg Asn 245 250 255

Val His Wing

<210> 147 <211> 1314 <212> DNA

<213> Saccharum officinarum <220>

<221> misc_feature <222> (416) .. (416) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (881) .. (881) <223> η is a, c, g, or t <4 00> 147

attcaaattc aaatacctgg ggtttggagg gaatggtaca ggttattcgt cagggaaatt 60

gcgagtaaac tcgtccacac catcaaaatg gtgttaacca ccatacgttc cgtgcaagtt 120 aggaaaggaa aaccacgggc tacagaaaat tttgatgaag gaagtagata tgaagtcatt 180 gatgaaatgt ggacagacaa tatatcagga acttcatctt ctgtaagtac agctgatgga 240 agatcctttg agcaagataa agctgaacga attctgttgc aagactccaa attggcactt 300 ggtattaggg agatattggg acttgtccga acactcggtg aaggttgtag gctttcttgc 360 ttgtttaagt gccatgaagc cttggaagtc tacagaagac tccctgagac ccattntagc 420 actggatgga gcatatgcca ggttggtaag gcatatttcg aattagttga ttatttggaa 480 gctgatcgtt actttgaatt ggcacaccga ctgtcgcctt gtacgcttga tggaatggac 540 atctattcta ctgttcttta tcatctgaat gaggaaatga gactaagcta ccttgctcaa gagcttattt ccattgatcg actatctcct caagcatggt gtgcagtggg caattgcttt gccttgagga aagatcatga gactgctttg aagaattttc aacgttcggt acagcttgac tcaagatttg catatgctca cactctatgt ggtcatgagt attctgcatt ggaggattat gagaatagta tcaaattcta ccggtgtgca ctgcaggtag atgaaaggca ctacaatgcc tggtatggcc ttggggtggt gtatcttcgc caggaaaagt ntgagtttgc tgagcatcat ttcagaaggg catttcagat aaatcctcgc tcttctg ttc tcatgtgcta tcttgggatg gcgttgcatt ctcttaagag gaaggaggag gcattggaaa tgatggagaa agctatagca gctgataaga agaatccact gcccaagtat cagaaggcct taatccttct aggtcttcag aagtatcaag aagctctgga tgagttggag cggctaaagg agattgcacc tcatgagagc agtatgtatg cactgatggg aaagatttac aagcaactca atatccttga caaagctgtt ttctgctttg gcattgccct ggatttgaaa cctcctgctg ctgatcttgc tataattaag tccgcaatgg agaaagtaca tctccctgat gaactgatgg aggatgacct GTAA

<210> 148 <211> 437 <212> PRT

<213> Saccharum officinarum <220>

<221> UNSAFE <222> (139) .. (139)

<223> Xaa can be any naturally occurring amino acid <220>

<221> UNSAFE <222> (294) .. (294)

<223> Xaa can be any naturally occurring amino acid <400> 148

Glle Ile Glle Ile Pro Gly Val Trp Arg Glu Trp Tyr Arg Leu Phe 1 5 10 15

Val Arg Glu Ile Wing Ser Lys Leu Val His Thr Ile Lys Met Val Leu

20 25 30

Thr Thr Ile Arg Be Val Gln Val Arg Lys Gly Lys Pro Arg Wing Thr

35 40 45

Glu Asn Phe Asp Glu Gly Ser Arg Tyr Glu Val Ile Asp Glu Met Trp

50 55 60

Thr Asp Asn Ile Be Gly Thr Be Be Val Be Thr Wing Asp Gly 65 70 75 80

Arg Ser Phe Glu Gln Asp Lys Glu Wing Arg Ile Leu Read Gln Asp Ser

85 90 95

Lys Leu Wing Leu Gly Ile Arg Glu Ile Leu Gly Leu Val Arg Thr Leu

100 105 110

Gly Glu Gly Cys Arg Leu Be Cys Leu Phe Lys Cys His Glu Wing Leu

115 120 125

Glu Val Tyr Arg Arg Read Leu Glu Thr His Xaa Ser Thr Gly Trp Ser

130 135 140

Ile Cys Gln Val Gly Lys Wing Tyr Phe Glu Leu Val Asp Tyr Leu Glu 145 150 155 160

Asp Arg Wing Tyr Phe Glu Read His Arg Wing Read Be Pro Cys Thr Leu

165 170 175

Asp Gly Met Asp Ile Tyr Ser Thr Val Leu Tyr His Leu Asn Glu Glu

180 185 190

Met Arg Leu Ser Tyr Leu Wing Gln Glu Leu Ile Ser Ile Asp Arg Leu

195 200 205

Ser Pro Gln Wing Trp Cys Wing Val Gly Asn Cys Phe Wing Read Arg Lys

210 215 220

Asp His Glu Thr Wing Leu Lys Asn Phe Gln Arg Ser Val Gln Leu Asp 225 230 235 240

Ser Arg Phe Ala Tyr Ala His Thr Read Cys Gly His Glu Tyr Ser Ala

245 250 255

Glu Asp Tyr Glu Asn Ser Ile Lys Phe Tyr Arg Cys Wing Glu Asu

260 265 270

Val Asp Glu Arg His Tyr Asn Wing Trp Tyr Gly Leu Gly Val Val Tyr

275 280 285

Leu Arg Gln Glu Lys Xaa Glu Phe Ala Glu His His Phe Arg Arg Ala

600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1314 290 295

Phe Gln Ile Asn Pro Arg Be Ser Val Leu Met 305 310 315

Wing Read His Ser Read Lys Arg Lys Glu Glu Wing

325 330

Lys Wing Ile Wing Wing Asp Lvs Wing Lys Asn Pro Leu

340 345

Wing Leu Ile Leu Leu Gly Leu Gln Lys Tyr Gln

355 360

Read Glu Arg Read Leu Lys Glu Ile Wing Pro His Glu

370 375

Read Met Gly Lys Ile Tyr Lys Gln Read Asn Ile 385 390 395

Phe Cys Phe Gly Ile Wing Leu Asp Leu Lys Pro

405 410

Wing Ile Ile Lys Ser Wing Met Glu Lys Val His

420 425

Met Glu Asp Asp Leu

435 <210> 149 <211> 54 <212> DNA

<213> Artificial sequence <220>

<223> initiator: prm00778 <400> 149

ggggacaagt ttgtacaaaa aagcaggctt <210> 150 <211> 49 <212> DNA

<213> Artificial sequence <220>

<223> initiator: prm00779 <400> 150

ggggaccact ttgtacaaga aagctgggtt <210> 151 <211> 1042 <212> DNA <213> Oryza sativa <400> 151

ggggagttag gaaccttgac atacaaccaa tgataccatt tacagaggtt catatttttt agatggctca ttagtgatat gtacggccac gggtgatggc ctgaactaat attttattcg ctaaagtaca cgcaagattc aacggaaaaa agaaaccgat atgaaataac tagatcaact catgtcgtca aaaacaaaag cacgctgatg attcgatcat caaacaaagg tggtagtagt tcatcagaaa gaagaattaa agaaaaaact aatcccgtct ctacaaaagc cactcctttg atttgacatg caaaagcaag tacaactaca caacactgtc tctctatctc caaaggcagt tttcctctct acctctaatg atagcttgga gcaagttcaa tctaattcat ctataatcaa tctaatagct tattcataca taatatctgg tcccatctat catacacact gagtctgtgc tagcccgctg ctcttctctc ttcatttatc ttcttaaaat gcttatagct tgatattgag agggagagga gtgagagcta aacaacaagg cacactcttc ctacctcttc tccggttctt gtccaagaac ttcacctcaa tagctcgagc tacggcctaa gctcgatcgc tgcaccaata cttcactgga gatcgcctag ctgcgtgtct tgagcttctc tc <210> 152 <211> 951 <212> DNA <213> Oryza sativa <400> 152

300

Cys tyr

Read Glu

Pro lys

Glu Wing 365 Ser Ser 380

Leu Asp Pro Wing Leu Pro

Read Gly Met 320

Met Met Glu

335 Tyr Gln Lys 350

Read Asp Glu

Met Tyr Wing

Lys Wing Val 400

Wing Asp Leu 415

Asp Glu Leu 430

cacaatgcaa caactgtcaa cttc

ggagtagcta tggtttcac

ttttttcaag tttagtcaaa aggtgccgct cgatcgagat atgctcatct agtaaagcgt cgcgagccag gctccactcc agctgtattg tagtatagct atagttatat tatagctgac atatttgcag gctcagctca ccttt

cagctagagg aatttcagaa acatcatcgt cagttagtta atggacaaca atcgtgtttc agaaaattcc tctactaccc gcttccagct aactactagc actacattat tacaaatctg ctggcttatg gctcaaggta ctttctcttc

cttttgcgtt gcgcaggaga ctgcagctag ctagcttatc

54

49

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1042 atggatccgg tcacggcatc aatacacggt caccatcttc ctccaccgtt caacacccgc 60

gacttccatc accatctcca gcagcagcag caccagctgc atctcaagac cgaggatgac 120

caaggcggcg gcactccggg tgtcttcggc agccgcggca ccaagcgcga ccacgacgac 180

gacgagaaca gtggcaacgg ccatggaagc ggtggtgacg gcggtgacct cgcgctggta 240

cccccctcgg gtggcgggcc ggacggcgcc gggagcgaga gcgccacgcg ccgcccgagg 300

ggacgcccgg cggggtccaa gaacaagccg aagccaccga tcatcatcac cagggacagc 360

gccaacacgc tccggacgca tgtcatggag gtggccggcg gctgcgacat ctccgagagc 420

atcaccacgt tcgcgcgacg ccggcagcgc ggggtttgcg tgctcagcgg cgccggcacc 480

gtcactaacg tcacgctgcg gcagcccgca tcgcagggag cggtcgttgc gctccacggc 540

cggttcgaga tactctccct ctccggctcc ttcctcccgc cgcccgcccc gccggaggcc 600

acggggctca ccgtctacct ggccggaggc cagggccagg tcgtgggcgg cagcgtcgtc 660

ggcgcgctga ccgcggctgg gcctgtggtg ataatggcgg cgtcttttgc gaacgcggtg 720

tacgagcggc tgccgttgga ggacgacgag ctactggcgg ctcaagggca agccgacagc 780

gctgggttgc tcgccgcggg gcagcaagcg gcgcagctcg ccggcggggc cgtcgatcca 840

agcctcttcc aaggactacc accaaaccta ctcggaaacg tgcagctgcc gccggaagcc 900

gcctacggat ggaaccctgg cgccggcggt ggccgcccgg cgccgttctg a 951 <210> 153

<211> 316 <212> PRT <213> Oryza sativa <4 00> 153 Met Asp Pro Val Thr Ala Ser Ile His Gly His His Leu Pro Pro 1 5 10 15 Phe Asn Thr Arg Asp Phe His His Leu Gln Gln Gln Gln His Gln 20 25 30 Leu His Leu Lys Thr Glu Asp Asp Gln Gly Gly Gly Thr Pro Gly Val 35 40 45 Phe Gly Being Arg Gly Thr Be Gly Gly Asp Gly Gly Asp Leu Wing Leu Val 65 70 75 80 Pro Pro Be Gly Gly Gly Pro Asp Gly Wing Gly Be Glu Be Wing Thr 85 90 95 Arg Arg Pro Arg Gly Arg Wing Gly Be Lys Asn Lys Pro Lys Pro 100 105 110 Pro Ile Ile Ile Thr Arg Asp Be Wing Asn Thr Read Le Arg Thr His Val 115 120 125 Met Glu Val Wing Gly Gly Cys Asp Ile Be Glu Ser Ile Thr Thr Phe 130 135 140 Wing Arg Arg Arg Gln Arg Gly Val Cys Val Leu Ser Gly Wing Gly Thr 145 150 155 160 Val Thr Asn Val Thr Leu Arg Gln Pro Wing Be Gln Gly Val Val Wing 165 170 175 Wing Read His Gly Arg Phe Glu Ile Read Le Be Ser Gly Be Phe Leu 180 185 190 Pro Pro Wing Pro Pro Glu Wing Thr Gly Leu Thr Val Tyr Leu Wing 195 200 205 Gly Gly Gln Gly Gln Val Val Gly Gly Val Val Gly Val Wing Gly Leu Thr 210 215 220 Wing Gly Pro Val Val Ile Met Wing Wing Be Phe Wing Asn Wing Val 225 230 235 240 Tyr Glu Arg Leu Pro Leu Glu Asp Glu Leu Leu Wing Wing Gln Gly 245 250 255 Gln Wing Asp Ser Wing Gly Leu Leu Wing Wing Gly Wing Gln Gln Wing Wing Gln 260 265 270 Leu Wing Wing Gly Gly Wing Val Asp Pro Ser Leu Phe Gln Gly Leu Pro 275 280 285 Asn Val Gln Leu Pro Pro Glu Wing Tyr Gly Wing Trp 290 295 300 Asn Pro Gly Wing Gly Gly Gly Arg Wing Pro Phe 305 310 315 <210> 154 <211> 918 <212> DNA

<213> Oryza sativa

<4 00> 154

atggcaggtc tcgacctcgg caccgccgcg acgcgctacg tccaccagct ccaccacctc 60

caccccgacc tccagctgca gcacagctac gccaagcagc acgagccgtc cgacgacgac 120 cccaacggca gcggcggcgg cggcaacagc aacggcgggc cgtacgggga ccatgacggc 180

gggtcctcgt cgtcaggccc tgccaccgac ggcgcggtcg gcgggcccgg cgacgtggtg 240

gcgcgccggc cgcgggggcg cccgcctggc tccaagaaca agccgaagcc gccggtgatc 300

atcacgcggg agagcgccaa cacgctgcgc gcccacatcc tggaggtcgg gagcggctgc 360

gacgtgttcg agtgcgtctc cacgtacgcg cgccggcggc agcgcggcgt gtgcgtgctg 420

agcggcagcg gcgtggtcac caacgtgacg ctgcgtcagc cgtcggcgcc cgcgggcgcc 4 80

gtcgtgtcgc tgcacgggag gttcgagatc ctgtcgctct cgggctcctt cctcccgccg 540

ccggctcccc ccggcgccac cagcctcacc atcttcctcg ccgggggcca gggacaggtc 600

gtcggcggca acgtcgtcgg cgcgctctac gccgcgggcc cggtcatcgt catcgcggcg 660

tccttcgcca acgtcgccta cgagcgcctc ccactggagg aggaggaggc gccgccgccg 720

caggccggcc tgcagatgca gcagcccggc ggcggcgccg atgctggtgg catgggtggc 780

gcgttcccgc cggacccgtc tgccgccggc ctcccgttct tcaacctgcc gctcaacaac 840

atgcccggtg gcggcggctc acagctccct cccggcgccg acggccatgg ctgggccggc 900

gcacggccac cgttctga 918 <210> 155 <211> 305 <212> PRT

<213> Oryza sativa <4 00> 155

Met Wing Gly Leu Asp Leu Gly Thr Wing Ala Thr Arg Tyr Val His Gln 1 5 10 15 Leu His His Leu His Pro Asp Leu Gln Leu Gln His Ser Tyr Ala Lys 20 25 30 Gln His Glu Pro Ser Asp Asp Pro Asn Gly Be Gly Gly Gly Gly 35 40 45 Asn Be Asn Gly Gly Pro Tyr Gly Asp His Asp Gly Gly Ser Be Ser 50 55 60 Be Gly Pro Wing Thr Asp Gly Val Wing Gly Gly Pro Val Gly Asp Val 65 70 75 80 Wing Arg Arg Pro Arg Gly Arg Pro Pro Gly Be Lys Asn Lys Pro Lys 85 90 95 Pro Pro Val Ile Ile Thr Arg Glu Be Wing Asn Thr Read Arg Wing His 100 105 110 Ile Leu Glu Val Gly Be Gly Cys Asp Val Phe Glu Cys Val Ser Thr 115 120 125 Tyr Wing Arg Arg Arg Gln Arg Gly Val Cys Val Leu Ser Gly Ser Gly 130 135 140 Val Val Thr Asn Val Thr Leu Arg Gln Pro Ser Wing Pro Gly Wing 145 150 155 160 Val Val Ser Leu His Gly Arg Phe Glu Ile Leu To Be Leu To Be Gly Ser 165 170 175 y Wing Thr Be Leu Thr Ile Phe 180 185 190 Leu Wing Gly Gly Gln Gly Gln Val Val Gly Gn Asn Val Val Gly Wing 195 200 205 Leu Tyr Wing Gly Pro Val Ile Wing Wing Ser Phe Wing Asn 210 215 220 Val Wing Tyr Glu Arg Leu Pro Leu Glu Glu Glu Glu Wing Pro Pro Pro 225 230 235 240 Gln Wing Gly Leu Gln Met Gln Pro Gly Gly Wing Asp Wing Gly 245 250 255 Gly Met Gly Gly Wing Phe Pro Pro Asp Pro Be Wing Wing Gly Leu Pro 260 265 270 Phe Phe Asn Leu Pro Leu Asn Asn Met Pro Gly Gly Gly Gly Ser Gln 275 280 285 Leu Pro Gly Wing Asp Gly His Gly Trp Wing Gly Wing Pro Pro 290 295 300 300

Phe 305

<210> 156

<211> 906

<212> DNA

<213> Lotus

<400> 156

atggatccat

caacaccaac

accccagcag

aacaacaaca

agaagacctc

acccgcgaca

atcgtcgaca

ggcactggaa

accctccacg

ccaccggcag

ggaagtgttg

agcaacgctg

ggaggttcaa

cagcagcaac

cttctcaatt

tactga

<210> 157

<211> 301

<212> PRT

<213> Lotus

<400> 157

corniculatus

tatcagcaca accagcagca aagatgaaca gccatgacgg gaggaaggcc gcgccaacgc gcgtctccaa ccgtcaccaa gaaggtttga catcaggttt tgggagctct cgtatgagag tgggttcacc agcttttcag ctg

cggccactct gcatcagcag gagtggaagc caaagaaggc cgccggatcg acttaagacc ctttgcaaga cgtcactctc gattctctcc gaccatttac cattgcttcg gcttcctttg acccggtggt ggatgcacc gccaact

cttcctcctc catcagttcc agcggcggca tccggaggcg aagaacaagc cacgtcatgg cgccgccagc aggcagccag ctggcaggat ttggctggtt ggacctgtgg gaagatgagg agtggtggtg gcct

cttttctcca actctttaca tcaaaaggga gaggcgaaag ctaagccgcc aggtcgccga gcggcgtctg cttcttccgg cgttcctgcc gacaagggca ttatcatggc acccttcatt gtggtggagt ttggcatctg

tctgcaccac gcagcagcaa acgcgatgaa cgagaattca catcatcatc cggctgcgac catcatgagc cgctgttgtc gccgcctgct ggttgttgga agcttcgttc ggcaatgcaa tggtcagcaa cccctcctcc

corniculatus

Met Asp Pro Read His Ala His Gly His His Leu Pro Pro His Phe Leu 1 5 10 15 His Leu His His Gln His Gln His Gln His Gln His Gln His Gln His Gln 20 25 30 Phe His His Leu His Gln Gln Gln Thr Pro Ala Glu Asp Glu Gln Be 35 40 45 Gly Be Ser Gly Gly Ile Lys Arg Glu Arg Asp Glu Asn Asn Asn Ser 50 55 60 His Asp Gly Lys Glu Gly Gly Gly Gly Glu Be Glu Asn Ser 65 70 75 80 Arg Arg Pro Arg Gly Arg Pro Wing Gly Be Lys Asn Lys Pro Lys Pro 85 90 95 Pro Ile Ile Ile Thr Arg Asp Be Wing Asn Wing Read Le Lys Thr His Val 100 105 110 Met Glu Val Wing Asp Gly Cys Asp Ile Val Asp Be Val Ser Asn Phe 115 120 125 Wing Arg Arg Arg Gln Arg Gly Val Cys Ile Met Be Gly Thr Gly Thr 130 135 140 Val Thr Asn Val Thr Read Leu Arg Gln Pro Wing Be Gly Wing Val Val 145 150 155 160 Thr Read His Gly Arg Phe Glu Ile Leu Be Leu Wing Gly Ser Phe Leu 165 170 175 Pro Pro er Gly Leu Thr Ile Tyr Leu Wing 180 185 190 Gly Gly Gln Gly Gln Val Val Gly Gly Ser Val Val Gly Wing Leu Ile 195 200 205 Wing Ser Gly Pro Val Val Ile Met Wing Wing Be Phe Ser Asn Wing Wing 210 215 220 Tyr Glu Arg Leu Pro Leu Glu Asp Glu Asp Pro Be Leu Wing Met Gln 225 230 235 240 Gly Gly Be Met Gly Pro Pro Gly Gly Gly Gly Gly Gly 245 250 255 Val Gly Gln Gln Gln Gln Gln Leu Leu Gly Asp Wing Thr Wing Wing 260 265 270 Leu Phe His Gly Leu Pro Pro Asn Leu Leu Asn Ser Val Gln Met Pro 275 280 285

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 906 Asn Be Asp Asn Phe Trp Pro Be Gly Arg Be Pro Tyr

290 295 300

<210> 158 <211> 1020 <212> DNA

<213> Arabidopsis thaliana <400> 158

atggatccag ttcaatctca tggatcacaa agctctcttc gatttccaat tacatcttca acaacaacaa caacatcaac caacaacagt tctttctcca ccatcatcag caaccacaaa gagcagcaag gagggtcaat attgaataga tctatcaaga gataacatgg acaacatcgc taataccaac agcggtagcg cacggaggag aaggaggaag cggtggtgga ggaagtggag agaggaagac cagcaggatc caagaacaaa cctaaagctc agcgcaaacg cgcttcgaac tcacgtcatg gagataggag tgtatggcta cgttcgctag acgccgccaa agaggcgttt agcgttacta acgtcactat acgtcagcct ggatcgccac cacggccggt ttgaaatcct ctctctttcg ggatctttct gcagccaccg gactaagcgt ttacctagcc ggaggacaag gtggtgggac ctttgttgtg ttcgggtcct gtggtggtta gcggcgtacg aaaggctgcc tttggaagaa gatgagatgc ggtggaggag gaggaggtgg tggtggaatg ggatctcccc gctatggcag ctatggcggc ggctcaagga ctaccaccga ttgccaccgc cacaacagaa tgatcagcag tattggtcta <210> 159 <211> 339 <212> PRT

<213> Arabidopsis thaliana <400> 159

Met Asp Pro Val Gln Ser His Gly Ser Gln Ser 15 10

Phe His Wing Arg Asp Phe Gln Read His Leu Gln

20 25

Gln Gln Gln His Gln Gln Gln Gln Gln Gln Gln Gln

35 40

His Gln Gln Pro Gln Arg Asn Read Asp Gln Asp His

50 55 60

Gly Ser Ile Read Asn Arg Be Ile Lys Met Asp Arg 65 70 75

Asp Asn Met Asp Asn Ile Wing Asn Thr Asn Ser

85 90

Glu Met Ser Read His Gly Gly Glu Met Gly Ser

ctcctccttt ccatgctaga aacaacatca acaacaacaa

gaaaccttga tggatcgcga aaggtaaaga aacagatgac caataatcat acggatgtga gcgttatgag ctggctcggt tgcctccgcc ggcaggtcgt tggcggcttc agacgccagt cgatgatgggg cgggggg

tcaagatcac agagacaagc gatgagttta aagaaggcca aacaagagac catagttgac cggtacagga ggttagcctt tgcgccgcct tggaggtagt tcaaggaggc acagcaacaa ttcggttcag accgtattga

Be Leu Pro

Gln Gln Gln 30

Phe Phe Leu 45

Glu Gln

Gly Gly

Gly Glu Met Gln Arg Thr Arg Pro Arg Gly Arg Pro

100

105

115

120

Asn Lys Pro Lys Pro Wing Ile Ile Ile Thr Arg

130

135

Read Arg Thr His Val Met Glu Ile Gly Asp Gly

Asp 140 Cys

145

150

155

Cys Met Wing Thr Phe Wing Arg Arg Arg Gln Arg Gly

165 170

Be Gly Thr Gly Be Val Thr Asn Val Thr Ile Arg

180 185

Pro Pro Gly Be Val Val Be Read His Gly Arg Phe

195

200

Leu Ser Gly Ser Phe Leu Pro Pro Pro Wing Pro

210

215

Read Ser Val Tyr Read Wing Gly Gly Gln Gly Gln

Pro 220 Val

225

230

235

Val Val Gly Pro Read Leu Cys Ser Gly Pro Val Val

245 250

Ser Phe Ser Asn Wing Wing Tyr Glu Arg Leu Pro Leu

260

265

Glu Glu

Be glu

Gly Gly 110 Wing Gly 125

To be a wing

Asp Ile

Val cys

Gln Pro 190 Glu Ile 205

Wing wing

Val gly

Val met

Glu Glu 270

Pro Pro 15

Gln his

His his

Gln gly

Thr Ser 80 Gly Lys 95

Gly ser

To be lys

Asn wing

Val Asp 160 Val Met 175

Gly ser

Read Ser

Thr gly

Gly Ser 240 Wing 255 Wing

Asp Glu

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 Met Gln Thr 275 Pro Val Gln Gly Gly 280 Gly Gly Gly Gly 285 Gly Gly Gly Met 290 290 Gly Ser Pro Pro 295 Met Gly Gln Gln Gln 300 Wing Met Wing Wing Met Wing Wing Wing Wing Gln Gly Leu Pro Pro Asn Leu Leu Gly Ser Val Gln 305 310 315 320 Leu Pro Pro Gln 325 Gln Asn Asp Gln Gln 330 Tyr Trp Be Thr Gly 335 Arg Pro Pro Tyr

<210> 160 <211> 975 <212> DNA

<213> Arabidopsis thaliana <400> 160

atggatccag tacaatctca tggatcacaa agctctctac gactttcaat tacatcttca acaacagcaa caagagttct caaagaaacc aaaccgatgg tgaccaacaa ggaggatcag atggatcgtg aagagacaag cgacaacata gacaacatag ggtaaagaca tagatataca cggtggttca ggagaaggag catcagatga caagaagacc aagaggaaga ccagcgggat ccgattatca tcacacggga cagcgcaaac gcgcttagaa gatggctgcg acttagtcga aagcgttgcc acttttgcac tgcgttatga gcggtactgg aaatgttact aacgtcacta ccttctcctg gctcggtagt tagtcttcac ggaaggttcg tcttttctcc ctcctccggc tcctcctaca gccaccggat ggácaaggac aggtggttgg aggaagcgta gttggtccgt gttgtcatgg ctgcgtcttt Tagcaatgcg gcgtacgaaa gagatgcaga cgccggttca

<213> Arabidopsis thaliana <4 00> 161

Met Asp Pro Val Gln Ser His Gly Ser Gln Ser 15 10

Phe His Wing Arg Asp Phe Gln Read His Leu Gln

20 25

Phe Phe Read His His His Gln Gln Gln Arg Asn

35 40

Gln Gln Gly Gly Be Gly Gly Asn Arg Gln Ile

50 55

Glu Thr Be Asp Asn Ile Asp Asn Ile Wing Asn 65 70 75

Gly Lys Asp Ile Asp Ile His Gly Gly Ser Gly

85 90

Be Gly Gly Asp His Gln Met Thr Arg Arg Pro

100 105

Gly Ser Lys Asn Lys Pro Lys Pro Ile Ile

115 120

Wing Asn Wing Read Arg Thr His Val Met Glu Ile

ctcctccttt tcctccacca gaggaaaccg ctaacaacag gtggtggctc ccaagaacaa cccacgtgat gaagacgcca tacgtcagcc agattctatc tgagtgttta tgttatgtgc ggttgccttt cattggagtg gtcat

ccacgcaaga tcaccagcaa acaaatcaag cggtagtgaa cggaggagat accaaaacca ggagatcgga acgcggcgtt tggatctcat tctctcagga cctcgctgga tggtcctgtc agaggaagat gccgccagg

130

135

Leu Val Glu Ser Val Wing Thr Phe Wing Arg Arg 145 150 155

Cys Val Met Being Gly Thr Gly Asn Val Thr Asn

165 170

Pro Gly Be His Pro Be Pro Gly Be Val Val

180 185

Phe Glu Ile Leu To Be Leu To Be Gly To Be Phe Leu 195 200

To be read

Gln Gln

Gln thr

45 Lys Met 60

Asn ser

Glu Gly

Arg Gly

Ile Thr 125 Gly Asp 140

Arg Gln

Val thr

To be read

Pro Pro 205

Pro Pro Pro 15 Gln Gln Glu 30 Asp Gly Asp Asp Arg Glu Gly Be Glu 80 Gly Gly Gly 95 Arg Pro Wing 110 Arg Asp Be Gly Cys Asp Arg Gly Val 160 Ile Arg Gln 175 His Gly Arg 190 Pro Ala Pro

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 975 Pro Thr Wing Thr Gly Leu Ser Val Tyr Leu Wing Gly Gly Gln 210 215 220 Val Val Gly Gly Ser Val Val Gly Pro Leu Cys Wing Gly Pro Val 225 230 235 240 Val Val Val Wing Ala Ser Phe Ser Asn Wing Ala Tyr Glu Arg Leu Pro 245 250 255 Leu Glu Glu Asp Glu Met Gln Thr Pro Val His Gly Gly Gly Gly Gly 260 265 270 Gly Ser Leu Glu Ser Pro Pro Met Met Gly Gln Gln Leu Gln His Gln 275 280 285 Gln Gln Gln Wing Met Ser Gly His Gln Gly Leu Pro Pro Asn Leu Leu Gly 290 295 300 Ser Val Gln Leu Gln Gln His Asp Gln Ser Tyr Trp Ser Thr Gly 305 310 315 320

Arg Pro Prο Tyr

<210> 162 <211> 954 <212> DNA

<213> Arabidopsis thaliana <4 00> 162

atggatcagg tctctcgctc ccacaccatc aattccagca gaccagcacc gaatcggtgg cactcttcag ccggaaaaga ggaggaggag ataatcacat aaaccaaaac cgccaatcat atggaagtag caaacggatg caacgtggca tctgcgtttt ccagcttcag tacctggtgg ctttctctct cgggatcatt atttacttag ccggtggtca gcttcaggac ctgtagtgat ccgttggagg aagacgatca ggaaacgcaa caatgggtgg caacagttga tgcaagatcc tctgttcaat tgccagctga <210> 163 <211> 317 <212> PRT

<213> Arabidopsis thaliana <4 00> 163 Met Asp Gln Val Ser Arg Be Leu Pro Pro Phe Leu Be Arg Asp 1 5 10 15 Leu His Leu His 20 Pro His His Gln Phe 25 Gln His Gln Gln 30 Gln Gln Gln Gln Asn 35 His Gly His Asp Ile 40 Asp Gln His Arg Ile 45 Gly Gly Leu Lys Arg 50 Asp Arg Asp Wing Asp 55 Ile Asp Pro Asn Glu 60 His Ser Be Wing Gly Lys Asp Gln Ser Thr Gly Gly Gly 65 70 75 80 Gly Gly Gly Asp Asn 85 His Ile Thr Arg Arg 90 Pro Arg Gly Arg Pro 95 Wing Gly Ser Lys Asn 100 Lys Pro Pro 105 Ile Ile Ile Thr Arg 110 Asp Be Wing Asn Wing Leu Lys Being His Val Met Glu Val Wing Asn Gly Cys Asp

115 120 125

Val Met Glu Ser Val Thr Val Phe Wing Arg Arg Arg Gln Arg Gly Ile

130 135 140

Cys Val Leu Ser Gly Asn Gly Wing Val Thr Asn Val Thr Ile Arg Gln 145 150 155 160

Pro Wing Being Val Pro Gly Gly Gly Being Val Val Asn Read His Gly

tcttcctcca tcagcagcag gctaaaacgt tcaaagtact cacgagaagg catcactcga tgacgtcatg gagcggaaac tggctcatct ccttcctcct gggacaggtt tatggcagct agaagagcaa tggaacgcagggggggggtgg

ccttttctct cagcagcaac gaccgagatg cctggctccg ccacgtggca gacagcgcaa gaaagtgtca ggcgccgtta gtcgttaact ccggctccac gttggaggaa tcgtttggaa acagcgaggag acgga

caagagatct agaatcacgg ctgatatcga gtggagaaag gaccagcggg acgctctcaa ccgtcttcgc ccaacgttac tacacggacg cagctgcgtc gcgtggttgg acgctgcgta cggttgccca tga

ccatcttcac ccacgatata tcccaacgag cggcggcgga atctaagaac atctcatgtc tcgccgtcgc cataagacaa tttcgagatt aggtctaacg tccactcatg tgagagactg taatatcgat gcaacagcaa tcttatgaat cta

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 954 165 170 175 Arg Phe Glu Ile 180 Read Be Read Gly 185 Be Phe Read Pro Pro 190 Pro Wing Pro 195 Wing Be Gly Leu Thr 200 Ile Tyr Leu Wing Gly 205 Gly Gln Gly Gln Val 210 Val Gly Gly Ser Val 215 Val Gly Pro Leu Met 220 Wing Ser Gly Pro Val Val Ile Met Wing Wing Ser Phe Gly Asn Wing Tyr Glu Arg Leu 225 230 235 240 Pro Leu Glu Glu Asp 245 Asp Gln Glu Glu Gln 250 Thr Wing Gly Wing Val 255 Wing Wing Asn Asn Ile Asp 260 Gly Wing Asn Wing Thr Met 265 Gly Gly Finger Gln 270 Thr Gln Thr Finger 275 Gln Gln Finger Gln 280 Gln Finger Leu Asp Pro Thr Be Phe 290 Ile Gln Gly Leu Pro 295 Pro Asn Leu Met Met Asn 300 Ser Val Gln Leu Pro Wing Glu Wing Tyr Trp Gly Thr Pro Arg Pro Be Phe 305 310 315 <210> 1 (54

<211> 798 <212> DNA

<213> Arabidopsis thaliana <400> 164

atggatgagg tatctcgttc tcatacaccg caatttctat caagtgatca tcagcactat 60

caccatcaaa acgctggacg acaaaaacgc ggcagagaag aagaaggagt tgaacccaac 120 aatatagggg aagacctagc cacctttcct tccggagaag agaatatcaa gaagagaagg 180 ccacgtggca gacctgctgg ttccaagaac aaacccaaag caccaatcat agtcactcgc 240 gactccgcga acgccttcag atgtcacgtc atggagataa ccaacgcctg cgatgtaatg 300 gaaagcctag ccgtcttcgc tagacgccgt cagcgtggcg tttgcgtctt gaccggaaac 360 ggggccgtta caaacgtcac cgttagacaa cctggcggag gcgtcgtcag tttacacgga 420 cggtttgaga ttctttctct ctcgggttcg tttcttcctc caccggcacc accagctgcg 480 tctggtttaa aggtttactt agccggtggt caaggtcaag tgatcggagg cagtgtggtg 540 ggaccgctta cggcatcaag tccggtggtc gttatggcag cttcatttgg aaacgcatct 600 tacgagaggc tgccactaga ggaggaggag gaaactgaaa gagaaataga tggaaacgcg 660 gctagggcga ttggaacgca aacgcagaaa cagttaatgc aagatgcgac atcgtttatt 720 gggtcgccgt cgaatttaat taactctgtt tcgttgccag gtgaagctta ttggggaacg 780 798 caacgaccgt ctttctaa

<210> 165 <211> 265 <212> PRT

<213> Arabidopsis thaliana

<400> 165 Met Asp Glu Val Be Arg Be His Thr Pro Gln Phe Read Be Be Asp 1 5 10 15 His Gln His Tyr His His Gln Asn Wing Gly Arg Gln Lys Arg Gly Arg 20 25 30 Glu Glu Glu Gly Val Glu Pro Asn Asn Ile Gly Glu Asp Leu Wing Thr 35 40 45 Phe Pro Be Gly Glu Glu Asn Ile Lys Lys Arg Arg Arg Gly Arg 50 55 60 Pro Wing Gly Be Lys Asn Lys Pro Lys Ala Pro Ile Val Thr Arg 65 70 75 80 Asp Ser Wing Asn Wing Phe Arg Cys His Val Met Glu Ile Thr Asn Wing 85 90 95 Cys Asp Val Met Glu Being Read Ala Val Phe Wing Arg Arg Arg Gln Arg 100 105 110 Gly Val Cys Val Leu Thr Gly Asn Gly Val Val Thr Asn Val Thr Val 115 120 125 Arg Gln Pro Gly Gly Gly Val Val Be Read His Gly Arg Phe Glu Ile 130 135 140 Leu Be Leu Be Gly Be Phe Leu Pro Pro Wing Pro Pro Wing 145 150 155 160 Ser Gly Leu Lys Val Tyr Leu Wing Gly Gly Gln

165 170

Gly Ser Val Val Gly Pro Read Thr Wing Be Ser

180 185

Wing Wing Be Phe Gly Asn Wing Wing Be Tyr Glu Arg

195

200

Glu Glu Glu Thr Glu Arg Glu Ile Asp Gly Asn

210

215

Gly Thr Gln Thr Gln Lys Gln Read Met Gln Asp

Gly Gln

Pro val

Leu Pro 205 Wing 220 Wing

Thr wing

225

230

235

Gly Ser Pro Ser Asn Leu Ile Asn Ser Val Ser Leu Pro

245

250

Val Ile Gly

175 Val Val Met 190

Read Glu Glu

Arg Wing Ile

Ser Phe Ile 240

Gly Glu Wing 255

Tyr Trp Gly Thr Gln Arg Pro Ser Phe 260 265

<210> 166

<211> 933

<212> DNA

<213> Arabidopsis thaliana

<400> 166

atggcgaatc cttggtgggt agggaatgtt gcgatcggtg tcatcagctc cttctttgca ccacagaaac agtaacaaca cgttcggatc caagattgga ccatgacttc accaccaaca cagactcaga gccaagaaga acagaacagc agagacgagc tccggatccg ggtctacggg tcgtcgtcct agaggtagac ccaaagagtc cagttgttgt taccaaagaa agccctaact gagattgcta cgggagctga cgtggcggaa agcttaaacg cggggcgttt cggtgctgag cggtagtggt ttggttacta gctgcatccg gtggagttgt tagtttacgt ggtcagtttg gcttttcttc ctacgtctgg ctctcctgct gcagccgctg ggagctcaag gtcaagttgt gggaggtgga gttgctggcc gttattgtga tagctgctac gttttgcaat gccacttatg gaacaacagc aagagcagcc gcttcaacta gaagatggga gatgataacg agagtgggaa taacggaaac gaaggatcga atgcctccta attttatccc aaatggtcat caaatggctc ggtcctccgc ctcgtgctcc tccttcgtat tga

<210> 167

<211> 310

<212> PRT

<213> Arabidopsis thaliana

<400> 167

Met Asn Pro Wing Trp Trp Val Gly Asn Val Wing

gagttgagag acaacccacc acagtggaag aaccagctgt ctcctggttc ctctccagag cctttgctcg atgttactct agatcttgtc gtttaaccat cgcttattgc agaggttacc agaagcagaa tgcagccgcctac

tccagtgacg gactatgact ccctaatacc tgaacccgga caagaacaaa ccatgttctt tagacgcggc gcgtcagcct tatgtgtggg ttacttagct ctctggaccc gattgaggaa agaagagaat gatgtataat gtattgggt

Ile gly

Be Pro Val Thr Be Be Pro Wing Be Read His His Arg

10

Gly Val Glu 15

Asn Ser Asn

20 25 30 Asn Asn Asn 35 Pro Thr Met Thr 40 Arg Be Asp Pro Arg 45 Read Asp His Asp Phe 50 Thr Thr Asn Asn Ser 55 Gly Be Pro Asn Thr 60 Gln Thr Gln Be Gln Glu Gln Gln Asn Be Arg Asp Glu Gln Pro Wing Val Glu Pro Gly 65 70 75 80 Ser Gly Ser Gly Ser 85 Thr Gly Arg Arg Pro 90 Arg Gly Arg Pro Pro 95 Gly Ser Lys Asn Lys 100 Pro Lys Ser Pro Val 105 Val Val Thr Lys Glu 110 Ser Pro Asn Ser Leu 115 Gln Be His Val Leu 120 Glu Ile Wing Thr Thr Gly 125 Wing Asp Val Wing Glu 130 Ser Read Asn Wing Phe 135 Wing Arg Arg Arg Gly 140 Arg Gly Val Ser Val Leu Ser Gly Ser Gly Leu Val Thr Asn Val Thr Leu Arg Gln Pro 145 150 155 160 Wing Wing Be Gly Gly 165 Val Val Be Leu Arg 170 Gly Gln Phe Glu Ile 175 Leu Be Met Cys Gly Wing Phe Leu Pro Thr Be Gly Ser Pro Wing Wing

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 933 180 185 190

Gly Wing Leu Thr Ile Tyr Leu Gly Wing Gln Gly Wing Gln Val Val Gly

195 200 205 Gly Gly 210 Val Wing Gly Pro Leu 215 Ile Wing Ser Gly Pro 220 Val Ile Val Ile Wing Thr Thr Phe Cys Asn Wing Thr Tyr Glu Arg Leu Pro Ile Glu Glu 225 230 235 240 Glu Gln Gln Gln Glu 245 Gln Pro Leu Gln Leu 250 Glu Asp Gly Lys Lys 255 Gln Lys Glu Glu Asn 260 Asp Asp Asn Glu Ser 265 Gly Asn Gly Asn 270 Glu Gly Ser Met Gln 275 Pro Met Tyr Asn 280 Met Pro Asn Phe 285 His Gln Met Gln Wing His Asp Val Tyr Trp Gly Gly Pro Pro

290 295 300

Arg Wing Pro Pro Ser Tyr 305 310

<210> 168 <211> 987 <212> DNA <213> Oryza sativa <4 00> 168

atggatccgg tgacggcggc ggcggcgcat gggggtgggc accaccacca ccaccacttc 60

ggagcgccac cggtggcggc gttccaccac cacccgttcc accacggcgg cggggcgcac 120

tacccggcgg cgttccagca gtttcaggag gagcagcagc agcttgtggc ggcggcggcg 180

gcggctggtg ggatggcgaa gcaggagctg gtggatgaga gcaacaacac catcaacagc 240

ggcgggagca acgggagcgg cggggaggag cagaggcagc agtccgggga ggagcagcac 300

cagcaagggg cggcggcgcc ggtggtgatc cggcgtccca ggggccgccc cgccggctcc 360

aagaacaagc ccaagcctcc ggtcatcatc acgcgcgaca gcgccagcgc gctgcgggcg 420

cacgtcctcg aggtcgcctc cgggtgcgac ctcgtcgaca gcgtcgccac gttcgcgcgc 480

cgccgccagg tcggtgtctg cgtgctcagc gccaccggcg ccgtcaccaa cgtctccgtc 540

cggcagcccg gcgcgggccc cggcgccgtc gtcaacctca ccggccgctt cgacatcctc 600

tcgctgtccg gctccttcct cccgccgccg gcgcctccct ccgccaccgg cctcaccgtc 660

tacgtctccg gcggccaggg gcaggtcgtg ggcggcacgg tcgccggacc gctcatcgcc 720

gtcggccccg tcgtcatcat ggccgcctcg ttcgggaacg ccgcctacga gcgcctcccg 780

ctcgaggacg acgagccgcc gcagcacatg gcgggcggcg gccagtcctc gccgccgccg 840

ccgccgctgc cattaccacc acaccagcag ccgattcttc aagaccatct gccacacaac 900

ctgatgaacg gaatccacct ccccggcgac gccgcctacg gctggaccag cggcggcggc 960

ggcggcggcc gcgcggcgcc gtactga 987

<210> 169 <211> 328 <212> PRT

<213> Oryza sativa <4 00> 169 Met Asp Pro Val Thr Wing Ala Wing Ala His Gly Gly Gly His His 1 5 10 15 His His Phe 20 Gly Pro Pro Val 25 Phe Wing His 30 His Pro Phe His His 35 Gly Gly Gly Wing His 40 Tyr Pro Wing Phe Wing 45 Gln Phe Gln Glu 50 Glu Gln Gln Gln Leu 55 Val Wing Wing Wing 60 Wing Wing Gly Gly Met Wing Lys Gln Glu Read Val Asp Glu Be Asn Asn Thr Ile Asn Ser 65 70 75 80 Gly Gly Ser Asn Gly 85 Ser Gly Gly Glu Glu 90 Gln Arg Gln Gln Ser 95 Gly Glu Glu His 100 Gln Gln Gly Wing 105 Wing Pro Val Val Ile 110 Arg Arg Pro Arg Gly 115 Arg Pro Wing Gly Ser 120 Lys Asn Lys Pro Lys 125 Pro Pro Val Ile Ile 130 Thr Arg Asp Ser Wing 135 Ser Wing Leu Arg Wing 140 His Val Leu Glu Val Wing Ser Gly Cys Asp Leu Val Asp Ser Val Wing Thr Phe Ala Arg 97 145 150 155 160 Arg Arg Gln Val Gly Val Cys Val Leu Ser Wing Thr Gly Wing Val Thr 165 170 175 Asn Val Ser Val Arg Gln Pro Gly Wing Gly Pro Gly Wing Val Val Asn 180 185 190 Leu Thr Gly Arg Phe Asp Ile Leu To Be Leu To Be Gly To Be Phe Leu Pro 195 200 205 Pro Pro Wing To Pro To Be Wing Thr Gly Leu Thr Val Tyr Val To Gly 210 215 220 Gly Gln Gly Gln Val Val Val Gly Gly Thr Val Wing Gly Pro Leu Ile Wing 225 230 235 240 Val Gly Pro Val Val Ile Met Wing Al Ser Be Phe Gly Asn Wing Wing Tyr 245 250 255 Glu Arg Leu Pro Leu Glu Asp Asp Glu Pro Pro Gln His Met Wing Gly 260 265 270 Gly Gly Glly Be Ser Pro Pro Pro Pro Leu Pro Leu Pro Pro His 275 280 285 Gln Gln Pro Ile Leu Gln Asp His Leu Pro His Asn Leu Met Asn Gly 290 295 300 Ile His Leu Pro Gly Asp Wing Tyr Gly Wing Trp Thr Be Gly Gly Gly 305 310 315 320

Gly Gly Gly Arg Wing Pro Wing Tyr 325

<210> 170

<211> 777

<212> DNA

<213> Oryza sativa

<4 00> 170

atggggagca tcgacggcca ctcgctgcag cagcatcagg ggtactccca cggcggcggc 60

gcgggaggga gcaacgagga ggaggaggcg tcgccgccgc ccggcggtgg ctcggctacg 120 gggtcggcgg gccgccggcc gagggggagg ccgccgggct ccaagaacaa gccgaagccg 180 cccgtcgtgg tgacgcggga gagccccaac gcgatgcgtt cccacgtgct ggagatcgcc 240 agcggcgccg acatcgtcga ggccatcgcg ggcttctccc gccgcaggca gcgcggcgtc 300 tccgtgctca gcgggagcgg cgccgtcacc aacgtcacgc tccggcagcc cgcggggact 360 ggggccgccg ccgtcgcgct gcgggggagg ttcgagatat tgtccatgtc tggcgccttc 420 ctcccggcgc cggcgccgcc aggggccacg gggctcgccg tgtacctcgc cggcgggcag 480 gggcaggtgg tgggtgggag cgtcatgggg gagctgatcg cgtcgggccc cgtcatggtg 540 atcgcggcca cgttcggcaa cgccacgtac gagaggctgc cgctggacca ggaaggcgag 600 gagggcgccg tgctgtccgg gtcggagggc gccgccgcgc agatggagca gcagagcagc 660 ggaggcgccg tcgtgccccc gccgatgtac gccgccgtcc agcagacgcc gccgcacgac 720 atgttcgggc agtgggggca tgcagcggtg gctcggccgc cgccgacatc gttctag 777

<210> 171 <211> 258 <212> PRT

<213> Oryza sativa <4 00> 171 Met Gly Ser Ile Asp Gly His Ser Leu Gln His Gln Gly Tyr Ser 1 5 10 15 His Gly Gly Gly 20 Wing Gly Gly Ser Asn 25 Glu Glu Glu Glu Wing 30 Ser Pro Pro Gly 35 Gly Gly Be Ward Thr 40 Gly Be Ward Gly Arg 45 Arg Pro Arg Gly Arg 50 Pro Pro Gly Ser Lys 55 Asn Lys Pro Lys Pro 60 Pro Val Val Val Thr Arg Glu Be Pro Asn Wing Met Arg Be His Val Leu Glu Ile Wing 65 70 75 80 Ser Gly Wing Asp Ile 85 Val Glu Ile Wing 90 Gly Phe Ser Arg Arg 95 Arg Gln Arg Gly Val 100 Ser Val Leu Ser Gly 105 Ser Gly Wing Val Thr 110 Asn Val Thr Leu Arg 115 Gln Pro Wing Gly Thr 120 Gly Wing Wing Wing Val 125 Wing Wing Leu Arg Gly Arg Phe Glu Ile Leu Be Met Ser Gly Wing Phe Leu Pro Wing Pro 130 135 140

Pro Pro Wing Gly Wing Thr Gly Leu Wing Val Tyr Leu Wing Gly Gly Gln 145 150 155 160

Gly Gln Val Val Gly Gly Ser Val Met Gly Glu Leu Ile Wing Ser Gly

165 170 175

Pro Val Met Val Ile Wing Wing Thr Thr Phe Gly Wing Wing Thr Tyr Glu Arg

180 185 190

Leu Pro Leu Asp Gln Glu Gly Glu Glu Gly Wing Val Leu Ser Gly Ser

195 200 205

Glu Gly Wing Wing Wing Gln Met Glu Gln Gln Be Ser Gly Gly Wing Val

210 215 220

Val Pro Pro Pro Met Tyr Wing Val Val Gln Thr Pro Pro His Asp 225 230 235 240

Met Phe Gly Gln Trp Gly His Wing Val Wing Arg Wing Pro Pro Pro 245 250 255

To be Phe

<210> 172 <211> 798 <212> DNA

<213> Lycopersicon esculentum <4 00> 172

atggctggtt tggacttagg ttccgcttct catcatcgtt ttctccctcg tcatctccac 60

gatcctcaag acgatgaaat caatcgcaac aacactcaat tttccgatga tgacaacaac 120

aacaacgata acaataacaa taatagcccc ggggtagcac gtagacctag aggtcgtccc 180

gcggggtcca aaaataagcc taagcctccc gtgatcataa cacgggagag cgctaacgcg 240

ctccgtgcgc atattttaga agtgagtagc ggacatgatg tctttgaatc agttgctact 300

tatgctagaa aaagacaaag aggaatttgc atactgagcg ggagcggtac ggtgaataac 360

gtcaccatac ggcagccaca ggctgccggt tctgtggtga cgttacacgg aagattcgag 420

atattatctt tatccggatc tttcctacca ccacccgctc cccctggggc caccagctta 480

acgatttatt tagcgggtgg tcaaggtcaa gttgttggtg gaaacgttgt gggtgcgcta 540

attgcatcag gaccggttat tgttattgct tcgtcattta ctaatgttgc ttatgagaga 600

ttgcctttgg atgaagaaaa tgagtcaatt cagatgcagc aacaaggaca aagtggtaat 660

tttgctgatc catctaatat tggattacct tttcttaatt tgccattaaa catgccaaat 720

ggtggtggtc aactacaatt ggaaagtgga ggaggtgaag gttggaatgg aaacacaaca 780

aacaggccac aatattag 798 <210> 173 <211> 265 <212> PRT

<213> Lycopersicon esculentum <4 00> 173

Met Ala Gly Leu Asp Leu Gly Be Ala Be His His Arg Phe Leu Pro 1 5 10 15 Arg His Leu His 20 Asp Pro Gln Asp 25 Glu Ile Asn Arg Asn 30 Asn Thr Gln Phe Ser 35 Asp Asp Asp Asn 40 Asn Asn Asp Asn Asn 45 Asn Asn Asn Ser Pro 50 Gly Val Wing Arg Arg 55 Pro Arg Gly Arg Pro 60 Wing Gly Ser Lys Pro Lys Pro Lys Pro Val Ile Ile Thr Arg Glu Ser Wing Asn Wing 65 70 75 80 Leu Arg Wing His Ile 85 Leu Glu Val Ser Ser 90 Gly His Asp Val Phe 95 Glu Ser Val Val Wing Thr 100 Tyr Wing Arg Lys Arg 105 Gln Arg Gly Ile Cys 110 Ile Leu Ser Gly Ser 115 Gly Thr Val Asn 120 Val Thr Ile Arg Gln 125 Pro Gln Ala Wing Gly 130 Ser Val Val Thr Leu 135 His Gly Arg Phe Glu 140 Ile Leu Ser Leu Ser Gly Ser Phe Leu Pro Pro Ala Pro Pro Gly Ala Thr Ser Leu 145 150 155 160 Thr Ile Tyr Leu Ala 165 Gly Gly Gln Gly Gln 170 Val Val Gly Gly Asn 175 Val Val Gly Wing Leu Ile Wing Ser Gly Pro Val Ile

180 185

Phe Thr Asn Val Wing Tyr Glu Arg Leu Pro Leu

195 200

Ser Ile Gln Met Gln Gln Gln Gly Gln Be Gly

210 215

Ser Asn Ile Gly Leu Pro Phe Leu Asn Leu Pro 225 230 235

Gly Gly Gly Gln Gave Gln Gave Glu Ser Gly Gly

245 250

Gly Asn Thr Thr Asn Arg Pro Gln Tyr 260 265

<210> 174 <211> 813 <212> DNA

<213> Arabidopsis thaliana <4 00> 174

atggaactta acagatctga agcagacgaa gcaaaggccg gccaccagct cagccacagc ctctggctct tcctccggac gcaggttcca aaaacaaacc caaacctccg acgattataa cttagatcac acgttcttga agtcacctcc ggttcggaca tacgccactc gtcgcggctg cggcgtttgc attataagcg gtcacgatac ggcaacctgc ggctccggct ggtggaggtg tttgacattt tgtctttgac cggtactgcg cttccaccgc ggtttgacgg tgtatctagc cggaggtcaa ggacaagttg tcgttaattg cttcgggacc ggtagtgttg atggctgctt gataggttac cgattgaaga ggaagaaacc ccaccgccga cagcagccgg aggcgtctca gtcgtcggag gttacgggga tcaaacctcc aaggtggaaa tggtggagga ggtgttgctt atgaacaatt ttcaattctc cgggggagat atttacggta ggtggtggcg gtgcgactag acccgcgttt tag <210> 175 <211> 270 <212> PRT

<213> Arabidopsis thaliana <400> 175

Met Glu Read Asn Arg Be Glu Wing Asp Glu Wing 15 10

Pro Thr Gly Gly Ala Thr Be Ser Ala Thr Ala

20 25

Gly Arg Arg Arg Arg Gly Arg Arg Wing Gly Ser

35 40

Pro Pro Thr Ile Ile Thr Arg Asp Be Pro Asn

50 55

Val Leu Glu Val Thr Gly Ser Asp Ile Ser 65 70 75

Tyr Wing Thr Arg Arg Gly Cys Gly Val Cys Ile

85 90

Val Thr Wing Asn Val Thr Ile Arg Gln Pro Wing

100 105

Gly Val Ile Thr Read His Gly Arg Phe Asp Ile

115 120

Thr Wing Leu Pro Pro Pro Wing Pro Pro Gly Wing

130 135

Tyr Leu Wing Gly Gly Gln Gly Gln Val Val Gly 145 150 155

Ser Leu Ile Ala Ser Gly Pro Val Val Leu Met

165 170

Asn Wing Val Tyr Asp Arg Leu Pro Ile Glu Glu

180 185

Pro Arg Thr Thr Gly Val Gln Gln Gln Gln

195 200

Be Glu Val Thr Gly Be Gly Wing Gln Wing Cys

Val Ile Ser Ser 190

Asp Glu Glu Asn Glu 205

Asn Phe Ala Asp Pro 220

Read Asn Met Pro Asn 240

Gly Glu Gly Trp Asn 255

agaccactcc gtcgtccacg ctagagatag tatccgaggc gcacgggtgc tgattaccct ctgcaccacc taggagggaa cttttgcaaa gaaccaccgg gtggggccca tctacaatct tgagcggcgg

caccggtgga tggtcgtcct tcctaacgtc agtctccacc ggtcactaac gcatggtcgg gggagcagga tgtggctggt cgcagtttat ggtgcagcag ggcgtgtgag tggaatgaat tagcggag

Lys Wing Glu

Ser Gly Ser 30

Lys Asn Lys 45

Val Leu Arg 60

Glu Wing Val

Ile Ser Gly

Pro Wing 110 Wing

Leu Ser Leu

125 Gly Gly Leu 140

Gly Asn Val

Wing Wing Wing

Glu Glu Thr 190

Glu Ala Ser

205 Glu Ser Asn

Thr Thr 15

To be to be

Pro lys

To be his

Ser Thr 80 Thr Gly 95

Gly Gly

Thr gly

Thr val

Gly Wing 160 Phe 175 Wing

Pro Pro Gln Being Read Gln

60 120 180 240 300 360 420 480 540 600 660 720 780 813 210 215

Gly Gly Asn Gly Gly Gly Gly Val Wing Phe Tyr 225 230 235

Met Asn Asn Phe Gln Phe Ser Gly Gly Asp Ile

245 250

Gly Gly Gly Gly Gly Gly Gly Gly Wing Thr Arg 260 265

<210> 176 <211> 948 <212> DNA

<213> Arabidopsis thaliana <4 00> 176

atggcgaatc catggtggac aggacaagtg aacctatccg ggttcctctc agttaaagaa accagatctc cacatctcca ggtcacaata atcatcacca tcaccaagaa gtcgataaca gacaacttga gtggagacga ccacgagcca cgtgaaggag cgtccacgtg gacgtcctgc tggttccaag aacaaaccaa cgcgattctc caaatgctct caagagccat gtcatggaga atcgaaaccc tagctacttt tgctaggcgg cgtcaacgtg aatggcacag tggctaacgt caccctccgt caaccctcga cctggtggtg cggctgtttt ggctttacaa gggaggtttg tctttcttgc caggaccggc tccacctggt tccaccggtt ggtcaaggtc aggttgttgg aggaagcgtg gtgggcccat atgctgatcg ccgccacgtt ctctaacgcg acttacgaga gaggcagcag agagaggcgg tggtggaggc agcggaggag ggcggaggtt cgccactaag cagcggtgct ggtggaggcg gtgtataata tgccgggagaa tcttgtttct aatggtggca agcggccaag aagcttatgg <tg2> <tg2>

<213> Arabidopsis thaliana <4 00> 177

Met Wing Asn Pro Trp Trp Thr Gly Gln Val Asn 15 10

Thr Thr Pro Pro Gly Ser Be Gln Read Lys Lys

20 25

Be Met Asn Met Ala Met Asp Be Gly His Asn

35 40

Gln Glu Val Asp Asn Asn Asn Asn Asn Asp Asp Asp

50 55

Gly Asp Asp His Glu Pro Arg Glu Gly Wing Val 65 70 75

Arg Pro Arg Gly Arg Pro Wing Gly Ser Lys Asn

85 90

Ile Phe Val Thr Arg Asp Be Pro Asn Ala Leu

100 105

Glu Ile Wing Ser Gly Thr Asp Val Ile Glu Thr

115 120

Arg Arg Arg Gln Arg Gly Ile Cys Ile Read Ser

130 135

Wing Asn Val Thr Read Le Arg Gln Pro Ser Thr Wing 145 150 155

Pro Gly Gly Wing Val Val Wing Wing Wing Glu Gly Wing

165 170

Be Gave Thr Gly Be Phe Gave Pro Gly Pro Wing

180 185

Gly Leu Thr Ile Tyr Leu Wing Gly Gly Gln Gly

195 200

Ser Val Val Gly Pro Read Met Wing Gly Pro Wing

210 215

Wing Thr Phe Ser Asn Wing Thr Tyr Glu Arg Leu 225 230 235

220

Asn Leu Gly Met Asn 240

Tyr Gly Met Ser Gly 255

Pro Wing Phe 270

gcctcgaaac tgaacatggc acaacaacga ccgtagaagc agccaccgat tcgctagtgg gcatctgcat ccgctgccgt agattctttc taacgattta tgatggcagc gattgccatt tggttccggg acggtaacca gtgtta

gacgccgcct catggactca cgacgataga ccccacgcgc cttcgtcact gactgacgtc cttgagcgga tgcggcggct tttaaccggt cttagccggt aggtccggtg ggaggaggaa gcagctcgg aggact aggact

Leu Ser Gly Leu Glu 15 Pro Asp Leu His Ile 30 Asn His His His His 45 Arg Asp Asn Leu Ser 60 Glu Pro Thr Arg 80 Lys Pro Lys Pro 95 Lys Pro His Val Met 110 Leu Wing Thr Phe Ala 125 Gly Asn Gly Thr Val 140 Wing Val Wing Wing Wing Wing 160 Arg Phe Glu Ile Leu 175 Pro Pro Gly Ser Thr 190 Gln Val Val

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 948

240 Glu Wing Glu Wing Gly Gly Gly Gly Gly Gly Serly Gly Val Val Pro

245 250 255

Gly Gln Read Gly Gly Gly Gly Be Pro Pro Read Be Ser Gly Wing Gly Gly

260 265 270

Gly Asp Gly Asn Gln Gly Leu For Val Tyr Asn Met Pro Gly Asn Leu

275 280 285

Val Ser Asn Gly Gly Gly Gly Gly Gly Gly Gln Met Ser Gly Gln Glu

290 295 300

Wing Tyr Gly Trp Wing Gln Wing Arg Be Gly Phe 305 310 315

<210> 178 <211> 708 <212> DNA <213> Oryza sativa <4 00> 178

atggcgtcca aggagccaag cggcgaccac gaccacgaga tgaacgggac cagcgccggg 60

ggcggcgagc ccaaggacgg cgcggtggtg accggccgca accggcgccc ccgcggacgg 120 ccgccgggct ccaagaacaa gcccaagccg cccatcttcg tgacgcggga cagcccgaac 180 gcgctgcgca gccacgtcat ggaggtggcc ggcggcgccg atgtcgccga gtccatcgcg 240 cacttcgcgc -ggcggcggca gcgcggcgtc tgcgtgctca gcggggccgg caccgtgacc 300 gacgtggccc tgcgccagcc ggccgcgccg agcgccgtgg tggcgctccg tgggcggttc 360 gagatcctgt ccctgacggg gacgttcctg ccggggccgg cgccgccggg ctccaccggg 420 ctgaccgtgt acctcgccgg cgggcagggg caggtggtgg gcggcagcgt 480 ggtggggacg ctcaccgcgg cggggccggt catggtgatc gcctccacct tcgccaacgc cacctacgag 540 aggctgccgc tggatcagga ggaggaggaa gcagcggcag gcggcatgat ggcgccgccg 600 ccactcatgg ccggcgccgc cgatccacta cttttcggcg ggggaatgca cgacgccggg 660 cttgctgcat ggcaccatgc ccgccctccg ccgccgccgc cctactag 708

<210> 179 <211> 235 <212> PRT

<213> Oryza sativa <4 00> 179

Met Ala Ser Lys Glu Pro Ser Gly Asp His Asp His Glu Met Asn Gly 15 10 15

Thr Be Wing Gly Gly Gly Glu Pro Lys Asp Gly Wing Val Val Thr Gly

20 25 30

Arg Asn Arg Arg Arg Arg Gly Arg Pro Gly Ser Lys Asn Lys Pro

35 40 45

Lys Pro Pro Ile Phe Val Thr Arg Asp Be Pro Asn Wing Read Arg Be

50 55 60

His Val Met Glu Val Wing Gly Gly Wing Asp Val Wing Glu Ser Ile Wing 65 70 75 80

His Phe Wing Arg Arg Arg Gln Arg Gly Val Cys Val Leu Ser Gly Wing

85 90 95

Gly Thr Val Thr Asp Val Wing Read Arg Gln Pro Wing Wing Pro Be Wing

100 105 110

Val Val Wing Read Arg Gly Arg Phe Glu Ile Read Be Read Thr Gly Thr

115 120 125

Phe Leu Pro Gly Pro Pro Wing Gly Ser Thr Thr Gly Leu Thr Val Tyr

130 135 140

Leu Wing Gly Gly Gln Gly Gln Val Val Gly Gly Ser Val Val Gly Thr 145 150 155 160

Read Thr Wing Wing Gly Pro Val Val Val Ile Wing Ser Thr Phe Wing Asn

165 170 175

Wing Thr Tyr Glu Arg Leu Pro Leu Asp Gln Glu Glu Glu Glu Wing

180 185 190

Gly Wing Gly Met Met Pro Wing Pro Pro Read Met Gly Wing Wing Asp Wing

195 200 205

Pro Read Leu Phe Gly Gly Gly Met His Asp Wing Gly Leu Wing Wing Trp

210 215 220

His His Wing Arg Pro Pro Pro Pro Pro Pro Tyr 225 230 235

<210> 180 <211> 846 <212> DNA

<213> Arabidopsis thaliana <400> 180

atggcaaacc cttggtggac gaaccagagt ggtttagcgg gcatggtgga ccattcggtc 60 tcctcaggcc atcaccaaaa ccatcaccac caaagtcttc ttaccaaagg agatcttgga 120 atagccatga atcagagcca agacaacgac caagacgaag aagatgatcc tagagaagga 180 gccgttgagg tggtcaaccg tagaccaaga ggtagaccac caggatccaa aaacaaaccc 240 aaagctccaa tctttgtgac aagagacagc cccaacgcac tccgtagcca tgtcttggag 300 atctccgacg gcagtgacgt cgccgacaca atcgctcact tctcaagacg caggcaacgc 360 ggcgtttgcg ttctcagcgg gacaggctca gtcgctaacg tcaccctccg ccaagccgcc 420 gcaccaggag gtgtggtctc tctccaaggc aggtttgaaa tcttatcttt aaccggtgct 480 ttcctccctg gaccttcccc acccgggtca accggtttaa cggtttactt agccggggtc 540 cagggtcagg tcgttggagg tagcgttgta ggcccactct tagccatagg gtcggtcatg 600 gtgattgctg ctactttctc taacgctact tatgagagat tgcccatgga agaagaggaa 660 gacggtggcg gctcaagaca gattcacgga ggcggtgact caccgcccag aatcggtagt 720 aacctgcctg atctatcagg gatggccggg ccaggctaca atatgccgcc gcatctgatt 780 ccaaatgggg ctggtcagct agggcacgaa ccatatacat gggtccacgc aagaccacct 840 tactga 846

<210> 181 <211> 281 <212> PRT

<213> Arabidopsis thaliana <400> 181

Met Wing Asn Pro Trp Trp Thr Asn Gln Ser Gly 15 10

Read Wing

Asp His Be Val Ser Be Gly His His Gln Asn His His

20

25

Leu Leu Thr Lys Gly Asp Leu Gly Ile Ala Met

35

40

Asp Asp Gln Asp Glu Asp Asp Asp Pro Arg Glu

50 55

Val Asn Arg Arg Arg Arg Gly Arg Pro Gly 65 70 75

Lys Wing Pro Ile Phe Val Thr Arg Asp Ser Pro

85 90

His Val Leu Glu Ile Ser Asp Gly Ser Asp Val

100 105

His Phe Ser Arg Arg Arg Gln Arg Gly Val Cys

115 120

Gly Ser Val Wing Asn Val Thr Read Le Arg Gln Wing

130 135

Val Val Ser Leu Gln Gly Arg Phe Glu Ile Leu

Phe Leu Pro Pro Gly Pro Pro Pro Gly Ser Thr

165 170

Read Wing Gly Val Gln Gly Gln Val Val Gly Gly

180 185

Leu Leu Wing Ile Gly Ser Val Val Val Ile Wing

195 200

Thr Wing Tyr Glu Arg Leu Pro Met Glu Glu Glu

210 215

Ser Arg Gln Ile His Gly Gly Gly Asp Ser Pro 225 230 235

Asn Leu Pro Asp Leu Ser Gly Met Wing Gly Pro

245 250

Pro His Leu Ile Pro Asn Gly Wing Gly Gln Leu

260 265

Thr Trp Val His Wing Arg Pro Pro Tyr

275 280

<210> 182 <211> 879 <212> DNA

Asn gln

45 Gly Wing 60

To be lys

Asn wing

Asp wing

Val Leu 125 Wing Wing 140

To be read

Gly Leu

To be val

Wing Thr 205 Glu Asp 220

Pro Arg Gly Tyr Gly His

Gly Met Val 15

His Gln Ser 30

Be Gln Asp

Val Glu Val

Asn Lys Pro 80

Read Arg Ser 95

Thr Ile Wing 110

Be gly thr

Pro gly gly

Thr Gly Wing 160

Thr Val Tyr 175

Val Gly Pro 190

Phe Ser Asn

Gly Gly Gly

Ile Gly Ser 240

Asn met pro

255 Glu Pro Tyr 270 <213> Arabidopsis thaliana <400> 182 atggctggtc gatctccacc catttcaccg ggtggaggat ggtcgtcgtc atcacgcgcg gatgttttcg agcggtagcg gtgacgctac gcacctcccg ggaggaagcg tttactaatg ggaggaggat ggacttccgt ggttggccgg <210> 183 <211> 292 <212> PRT <213> Arabidopsis thaliana <400> 183

Met Ally Gly Leu Asp Leu Gly Thr Ala Phe Arg Tyr Val Asn His Gln 1 5 10 15 Leu His Arg Pro Asp Leu His Leu His His Asn Be Ser Asp Asp 20 25 30 Val Thr Pro Gly Ala Gly Met Gly His Phe Thr Val Asp Asp Glu Asp 35 40 45 Asn Asn Asn Asn Asn His Gln Gly Leu Asp Leu Wing Be Gly Gly Gly Be 50 55 60 Gly Be Gly Gly Gly Gly Gly Gly Gly Gly Asp Val Val 70 70 75 80 Gly Arg Arg Pro Gly Arg Pro Gly Pro Pro Gly As Lys Asn Lys Pro 90 90 95 Pro Pro Val Ile Ile Thr Arg Glu Be Wing Asn Thr Leu Arg Wing His 100 105 110 Ile Leu Glu Val Thr Asn Gly Cys Asp Val Phe Asp Cys Val Ala Thr 115 120 125 Tyr Wing Arg Arg Arg Gln Arg Gly Ile Cys Val Leu Be Gly Ser Gly 130 135 140 Thr Val Thr Asn Val Ser Ile Arg Gln Pro Be Wing Gly Wing Val 145 150 155 160 Val Thr Leu Gln Gly Thr Phe Glu Ile Read It Be Read It Be Gly Be Phe 165 170 175 Read It Pro Pro Pro Wing Pro Pro Gly A la Thr Be Leu Thr Ile Phe Leu 180 185 190 Wing Gly Gly Gln Gly Gln Val Val Gly Gly Ser Val Val Gly Glu Leu 195 200 205 Thr Wing Wing Gly Pro Val Ile Val Ile Wing Wing Be Phe Thr Asn Val 210 215 220 Wing Gyr Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gn Met Asn Met Gln 260 265 270 Pro Asn Val Gln Read Pro Val Glu Gly Trp Pro Gly Asn Ser Gly Gly 275 280 285

Arg Gly Pro Phe

290 <210> 184 <211> 918 <212> DNA

ttgatctagg cacagctttt cgttacgtta atcaccagct ccatcgtccc 60

ttcaccacaa ttcctcctcc gatgacgtca ctcccggagc cgggatgggt 120

tcgacgacga agacaacaac aacaaccatc aaggtcttga cttagcctct 180

caggaagctc tggaggagga ggaggtcacg gcgggggagg agacgtcgtt 240

cacgtggcag accaccggga tccaagaaca aaccgaaacc tccggtaatt 300

agagcgcaaa cactctaaga gctcacattc ttgaagtaac aaacggctgc 360

actgcgttgc gacttatgct cgtcggagac agcgagggat ctgcgttctg 420

gaacggtcac gaacgtcagc atacgtcagc catctgcggc tggagcggtt 480

aaggaacgtt cgagattctt tctctctccg gatcgtttct tcctcctccg 540

gagcaacgag tttgacaatt ttcttagccg gaggacaagg tcaggtggtt 600

ttgtgggtga gcttacggcg gctggaccgg tgattgtgat tgcagcttcg 660

ttgcttatga gagacttcct ttagaagaag atgagcagca gcaacagctt 720

ctaacggcgg aggtaatttg tttccggagg tggcagctgg aggaggagga 780

tctttaattt accgatgaat atgcaaccaa atgtgcaact tccggtggaa 840

ggaattccgg tggaagaggt cctttctga 879 <213> Oryza sativa <400> 184

atggcaggtc tcgacctcgg caccgccgcg acgcgctacg tccaccagct caccccgacc tccagctgca gcacagctac gccaagcagc acgagccgtc cccaacggca gcggcggcgg cggcaacagc aacggcgggc cgtacgggga gggtcctcgt cgtcaggtcc tgccaccgac ggcgcggtcg gcgggcccgg gcgcgccggc cgcgggggcg cccgcctggc tccaagaaca agccgaagcc atcacgcggg agagcgccaa cacgctgcgc gcccacatcc tggaggtcgg gacgtgttcg agtgcgtctc cacgtacgcg cgccggcggc agcgcggcgt agcggcagcg gcgtggtcac caacgtgacg ctgcgtcagc cgtcggcgcc gtcgtgtcgc tgcacgggag gttcgagatc ctgtcgctct cgggctcctt ccggctcccc ccggcgccac cagcctcacc atcttcctcg ccgggggcca gtcggcggca acgtcgtcgg cgcgctctac gccgcgggcc cggtcatcgt tccttcgcca acgtcgccta cgagcgcctc ccactggagg aggaggaggc caggccggcc tgcagatgca gcagcccggc ggcggcgccg atgctggtgg gcgttcccgc cggacccgtc tgccgccggc ctcccgttct tcaacctgcc atgcccggtg gcggcggctc acagctccct cccggcgccg acggccatgg gcacggccac cgttctga <210> 185 <211> 305 <212> PRT

<213> Oryza sativa <400> 185

Met Wing Gly Leu Asp Leu Gly Thr Wing Ala Thr Arg 15 10

Leu His His Leu His Pro Asp Leu Gln Leu Gln His

20 25

Gln His Glu Pro As Asp Asp Asp Pro Asn Gly Ser

35 40

Asn Ser Asn Gly Gly Pro Tyr Gly Asp His Asp Gly

50 55 60

Ser Gly Pro Wing Thr Asp Gly Wing Val Gly Gly Pro 65 70 75

Wing Arg Arg Pro Arg Gly Arg Pro Gly Ser Lys

85 90

Pro Pro Val Ile Ile Thr Arg Glu Be 'Wing Asn Thr

100 105

Ile Leu Glu Val Gly Ser Gly Cys Asp Val Phe Glu

115 120

Tyr Wing Arg Arg Arg Gln Arg Gly Val Cys Val Leu 130 135 140

Val Val Thr Asn Val Thr Read Arg Gln Pro Ser Wing 145 150 155

Val Val Ser Read His Gly Arg Phe Glu Ile Read Ser

165 170

Phe Leu Pro Pro Pro Pro Wing Gly Pro Wing Thr

180 185

Read Wing Gly Gly Gly Gln Gly Gly Val Gly Gly Asn

195 200

Leu Tyr Wing Gly Pro Wing Val Ile Val Ile Wing 210 215 220

Val Wing Tyr Glu Arg Leu Pro Leu Glu Glu Glu Glu 225 230 235

Gln Wing Gly Leu Gln Met Gln Gln Pro Gly Gly Gly

■ 245 250

Gly Met Gly Gly Wing Phe Pro Pro Asp Pro Be Wing

260 265

Phe Phe Asn Leu Pro Leu Asn Asn Met Pro Gly Gly

275 280

Read Pro Pro Gly Wing Asp Gly His Gly Trp Wing Gly 290 295 300

Phe 305

ccccacctc cgacgacgac ccatgacggc cgacgtggtg gccggtgatc gagcggctgc gtgcgtgctg cgcgggcgcc cctcccgccg gggacaggtc catcgcggcg gccgccgcggggggggg

Tyr Val His 15

Ser Tyr Wing 30

Gly Gly Gly 45

Gly Ser Ser

Gly Asp Val

Asn Lys Pro 95

Read Arg Wing 110 Cys Val Ser 125

Be gly be

Pro Wing Gly

Read Ser Gly 175

Read Thr Ile 190

Val Val Gly 205

Be Phe Ala

Pro Pro Wing

Wing Asp Wing 255

Wing Gly Leu

270 Gly Gly Ser 285

Arg Pro Wing

Gln

Lys

Gly

To be

Val 80 Lys

His

Thr

Gly

Wing 160 Ser

Phe

Allah

Asn

Pro 240 Gly

Pro

Gln

Pro

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 918 <210> 186 <211> 858 <212> DNA

<213> Arabidopsis thaliana <400> 186

atggctggtc tcgatctagg cacaacttct cgctacgtcc acaacgtcga tggtggcggc 60

ggcggacagt tcaccaccga caaccaccac gaagatgacg gtggcgctgg aggaaaccac 120

catcatcacc atcataatca taatcaccat caaggtttag atttaatagc ttctaatgat 180

aactctggac taggcggcgg tggaggagga gggagcggtg acctcgtcat gcgtcggcca 240

cgtggccgtc cagctggatc gaagaacaaa ccgaagccgc cggtgattgt cacgcgcgag 300

agcgcaaaca ctcttagggc tcacattctt gaagttggaa gtggctgcga cgttttcgaa 360

tgtatctcca cttacgctcg tcggagacag cgcgggattt gcgttttatc cgggacggga 420

accgtcacta acgtcagcat ccgtcagcct acggcggccg gagctgttgt gactctgcgg 480

ggtacttttg agattctttc cctctccgga tcttttcttc cgccacctgc tcctccaggg 540

gcgactagct tgacgatatt cctcgctgga gctcaaggac aggtcgtcgg aggtaacgta 600

gttggtgagt taatggcggc ggggccggta atggtcatgg cagcgtcttt tacaaacgtg 660

gcttacgaaa ggttgccttt ggacgagcat gaggagcact tgcaaagtgg cggcggcgga 720

ggtggaggga atatgtactc ggaagccact ggcggtggcg gagggttgcc tttctttaat 780

ttgccgatga gtatgcctca gattggagtt gaaagttggc aggggaatca cgccggcgcc 840

ggtagggctc cgttttag 858 <210> 187 <211> 285

<212> PRT <213> Arabidopsis thaliana <400> 187

Met Wing Gly Leu Asp Leu Gly Thr Thr Be Arg Tyr Val His Asn Val 1 5 10 15 Asp Gly Gly Gly Gly Gly Gln Phe Thr Asp Asn His His His His Asn His Asn 35 40 45 His His Gln Gly Leu Asp Leu Ile Ala Ser Asn Asp Asn As Gn Leu 50 55 60 Gly Gly Gly Gly Gly Gly Gly Gly Asp Leu Val Met Arg Pro 65 70 75 80 Arg Gly Arg Pro Wing Gly Ser Lys Asn Lys Pro Pro Lys Pro Val Ile 85 90 95 Val Thr Arg Glu Ser Wing Asn Thr Read Arg Wing His Ile Leu Glu Val 100 105 110 Gly Ser Gly Cys Asp Val Phe Glu Cys Ile Ser Thr Tyr Ala Arg Arg 115 120 125 Arg Gln Arg Gly Ile Cys Val Leu Ser Gly Thr Gly Thr Val Thr Asn 130 135 140 Val Ser Ile Arg Gln Pro Thr Wing Gly Wing Val Val Thr Leu Arg 145 150 155 160 Gly Thr Phe Glu Ile Leu Ser Leu Be Gly Ser Phe Leu Pro Pro 165 170 175 Wing Pro Pro Gly Wing Thr Be Leu Thr Ile Phe L i Wing Gly Wing Gln 180 185 190 Gly Gln Val Val Gly Gly Asn Val Val Gly Glu Leu Met Wing Wing Gly 195 200 205 Pro Val Met Val Wing Wing Ser Phe Thr Asn Val Wing Tyr Glu Arg 210 215 220 Leu Pro Leu Asp Glu His Glu His Glu His Leu Gln Be Gly Gly Gly Gly 225 230 235 240 Gly Gly Gly Gly Asn Met Tyr Be Glu Wing Thr Gly Gly Gly Gly Leu 245 250 255 Pro Phe Phe Asn Leu Pro Met Be Met Ser 260 265 270 Trp Gln Gly Asn His Wing Gly Wing Gly Wing Pro Wing Pro Phe 275 280 285 <210> 188 <211> 978 <212> DNA

<213> Medicago truncatula <4 00> 188

atggatcaag tagcacaagg tcgtcctctt ccacctccat cttcatcctc accatcaatt tcacaccaac caccaaacca ggcaatggga gcttaagccg aggccaaaaa agagaacgaa actcccaccg gaggaga.agg aaaagacgac ggtggtagcg ggtggtgaga tgggaagaag accaagagga agaccagcag ccacctatca tcatcacgag ggacagcgcg aacgcactcc gcaaatggat gtgacatcat ggaaagtgtg acggtctttg gtctgcatcc ttagcggaag tgggaccgtc acaaacgtga cctggtgcgg tagtcacact tcatggaaga tttgagatat ctgccgccgc ctgctccacc agcggcgtca ggattagcca ggacaggtcg tcggtggtag cgtggtggga ccgttgttag atggcagctt cctttggaaa tgctgcttat gaaaggctac ccagtgaatg tgccaggaaa tggagggtta gggtcaccgg caacagcagc agaaccagca acagcaacaa cttgtagcag ttccatggag ttcctcaaaa tcccccaat ggtggaagtg ctcggt12tctct2a2a

<213> Medicago truncatula <4 00> 189

ttctcactag atgaagagga acaacgaaga gaagtgctgg gttcgaaaaa gatcccacgt cgcgaaggag ctctccgtca tatcattatc tatatctagc cttcctagaaga gaaccatggg atcctaatgc taccagctga tcc

agaccttcat acaacaaagt cggcaacaac tggaggaagt caaaccaaaa gatggaagtt gcagcgtggt accagcatcg tggctctttc tggtggacaa ggttgttatc tgaagaaaca aagtcaacaa ttcatcactt aggttt

Met Asp Gln Val Vala Gln Gly Arg Pro Pro Leu Pro Pro Phe Leu Thr .1 5 10 15 Arg Asp Leu His Leu His Pro His His Gln Phe His Thr Asn His Gln 20 25 30 Thr Asn Glu Glu Glu Gln Gln Ser Gly Asn Gly Be Read Gly Arg 35 40 45 Gln Lys Arg Glu Arg Asn Asn Glu Asp Gly Asn Asn Thr Pro Thr Gly 50 55 60 Gly Glu Gly Lys Asp Asp Gly Gly Be Wing Gly Gly Gly Be 65 70 75 80 Gly Gly Glu Met Gly Arg Arg Pro Arg Gly Arg Pro Wing Gly Ser Lys 85 90 95 Asn Lys Pro Lys Pro Ile Ile Ile Thr Arg Asp Ser Wing Asn Wing 100 105 110 Leu Arg Be His Val Met Glu Val Wing Asn Gly Cys Asp Ile Met Glu 115 120 125 Ser Val Thr Val Phe Ala Arg Arg Arg Gln Arg Gly Val Cys Ile Leu 130 135 140 Ser Gly Ser Gly Thr Val Thr Asn Val Thr Leu Arg Gln Pro Wing Ser 145 150 155 160 Pro Gly Ala Val Val Thr He Read His Gly Arg Phe Glu Ile He Read Be Leu 165 170 175 Be Gly Be Phe Leu Pro Pro A la Pro Pro Wing Wing Ser Gly Leu 180 185 190 Wing Ile Tyr Leu Wing Gly Gly Gln Gly Gln Val Val Gly Gly Ser Val 195 200 205 Val Gly Pro Leu Wing Ser Gly Pro Val Val Ile Met Wing Ala Ser 210 215 220 Phe Gly Asn Ala Wing Tyr Glu Arg Leu Pro Leu Glu Asp Glu Glu Thr 225 230 235 240 Pro Val Asn Val Pro Gly Asn Gly Gly Leu Gly Pro Gly Thr Met 245 250 255 Gly Ser Gln Gln Gln Gln Gln Gln Gln Leu Val 260 265 270 Wing Asp Pro Asn Wing Be Ser Leu Phe His Gly Val Pro Gln Asn Leu 275 280 285 Leu Asn Ser Cys Gln Leu Pro Wing Glu Gly Tyr Trp Gly Gly Ser Wing 290 295 300

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 978 Arg 305

Ile met

<221> <222> <223> <400>

Gly Gly

Pro Pro Phe Leu Thr Lys Asn Val Ile His Leu Ile Thr 310 315

Phe Phe Val 325

<210> 190 <211> 6 <212> PRT

<213> Artificial sequence <220>

<223> reason 1 <220>

VARIANT (4). . (4)

/ replace = "Ile" 190

Gln Gly Gln Val 1

<210> 191 <211> 15 <212> PRT

<213> Artificial sequence <220>

<223> reason 2 <4 00> 191 Ile Leu Ser Leu 1

<210> 192 <211> 8 <212> PRT

<213> Artificial sequence <220>

<223> reason 3 <4 00> 192 Asn Wing Thr Tyr 1

<210> 193 <211> 12 <212> PRT <213> String <220>

<223> reason 4 <400> 193

Ser Phe Thr Asn Val Wing Tyr Glu Arg Leu Pro Leu

Phe

Read 320

To be 5

Gly Ser Phe Leu

Pro Pro 10

Pro Wing Pro

Pro 15

Glu Arg Leu Pro 5

artificial

1

<210> 194 <211> 42 <212> PRT <213> String <220>

<223> reason 5 <400> 194 Gly Arg Phe, Glu 1

Pro Pro Wing

10

artificial

Ile Leu Be Leu Thr Gly Be Phe Leu Pro Gly Pro

5 10 15

Gly Ser Thr Gly Leu Thr Ile Tyr Leu Wing Gly Gly Gln 20 25 30

Val Gly Gly Ser Val Val Gly 40

Gly Gln Val 35

<210> 195

<211> 654

<212> DNA

<213> Oryza sativa

<400> 195

cttctacatc ggcttaggtg tagcaacacg actttattat tattattatt attattatta Asn Ile Pro 15 Ser Asn Thr Lys 30 Arg His Phe Cys 45 Leu Arg Asn Val Gly Lys Asn Gly 80 Ser Ser Val Asn 95 His Gln Phe Pro

ttattttaca aaaatataaa atagatcagt ccctcaccac aagtagagca agttggtgag 120 ttattgtaaa gttctacaaa gctaatttaa aagttattgc attaacttat ttcatattac 180 aaacaagagt gtcaatggaa caatgaaaac catatgacat actataattt tgtttttatt 240 attgaaatta tataattcaa agagaataaa tccacatagc cgtaaagttc tacatgtggt 300 gcattaccaa aatatatata gcttacaaaa catgacaagc ttagtttgaa aaattgcaat 360 ccttatcaca ttgacacata aagtgagtga tgagtcataa tattattttc tttgctaccc 420 atcatgtata tatgatagcc acaaagttac tttgatgatg atatcaaaga acatttttag 480 gtgcacctaa cagaatatcc aaataatatg actcacttag atcataatag agcatcaagt 540 aaaactaaca ctctaaagca accgatggga aagcatctat aaatagacaa gcacaatgaa 600 aatcctcatc atccttcacc acaattcaaa tattatagtt gaagcatagt agta 654

<210> 196 <211> 51 <212> DNA

<213> Artificial sequence <220>

<223> primer <400> 196

ggggacaagt ttgtacaaaa aagcaggctt aaacaatgga tccggtcacg g 51

<210> 197 <211> 49 <212> DNA

<213> Artificial sequence <220>

<223> primer <400> 197

ggggaccact ttgtacaaga aagctgggtg gaatcgatcc atctcagaa 4 9

<210> 198 <211> 828 <212> DNA

<213> Arabidopsis thaliana <400> 198

atgggtggat cgatgtcgga gagagcaagg caggccaaca ttcctccact agcgggaccc 60

ctaaagtgtc ctcgatgcga ctccagcaac actaagttct gttactacaa caactataac 120 ctcactcagc ctcgtcactt ctgcaaaggt tgccgtcgct actggacaca agggggcgcc 180 ctgagaaacg tccctgtagg tggaggctgc cggaggaata acaagaaggg caaaaatgga 240 aatttaaaat cttcttcttc ttcgtccaaa cagtcttcct cggtcaacgc tcaaagtcct 300 agctcaggac agctaaggac aaatcatcag ttcccttttt caccaactct ttacaatctc 360 actcaactcg gaggtattgg tttgaactta gccgctacta atggcaacaa ccaagctcac 420 cagatcggtt ccagtttgat gatgagcgat ctagggtttc tccatggacg aaatacttca 480 actccgatga cgggaaacat tcatgaaaac aacaacaata ataacaatga aaacaaccta 540 atggcatccg ttggatcttt gagccccttt gctctcttcg atccaacgac ggggctatac 600 gctttccaga acgacggtaa tatcgggaac aacgttggga tatctggctc ttctacttcc 660 atggttgatt ctagggttta tcagacgcct ccggtgaaga tggaagaaca acctaatttg 720 gctaacttgt ctagaccggt ctccggtttg acgtctcctg ggaatcaaac aaatcagtac 780 ttttggcctg gttcggattt ctcgggtcct tctaatgatc 828 tcttgtga

<210> 199 <211> 275 <212> PRT

<213> Arabidopsis thaliana <400> 199

Met Gly Gly Be Met Be Glu Arg Wing Arg Gln Ali 15 10

Read Ally Gly Pro Read Lys Cys Pro Arg Cys Asp I Know

20 25

Phe Cys Tyr Tyr Asn Asn Tyr Asn Read Thr Gln Prc

35 40

Lys Gly Cys Arg Arg Tyr Trp Thr Gln

Pro Val Gly Gly Gly Cys Arg Asn Asn Lys Asn 65 70 75

Asn Leu Lys Being Being Being Being Being Being Lys Gln Sej

85 90

Gln Wing Be Pro Be Ser Gly Gln Leu Arg Thr Asr 100 105 110 Phe Be Pro 115 Thr Leu Tyr Asn Leu 120 Thr Gln Leu Gly Gly 125 Ile Gly Leu Asn Leu 130 Wing Ala Thr Asn Gly 135 Asn Gln Wing His 140 Gln Ile Gly Be Being Read Met Met Be Asp Read Gly Phe Read His Gly Arg Asn Thr Be 145 150 155 160 Thr Pro Met Thr Gly 165 Asn Ile His Glu Asn 170 Asn Asn Asn Asn Asn 175 Asn Glu Asn Asn Leu 180 Met Ala Ser Val Gly 185 Ser Leu Ser Pro Phe 190 Wing Leu Phe Asp Pro 195 Thr Thr Gly Leu Tyr 200 Wing Phe Gln Asn Asp 205 Gly Asn Ile Gly Asn 210 Asn Val Gly Ile Ser 215 Gly Ser Ser Thr Thr 220 Met Val Asp Ser Arg Val Tyr Gln Pro Pro Val Lys Met Glu Pro Gln Gln Pro Asn Leu 225 230 235 240 Wing Asn Leu Ser Arg 245 Pro Val Ser Gly Leu 250 Thr Ser Pro Gly Asn 255 Gln Thr Asn Gln Tyr 260 Phe Trp Pro Gly Ser 265 Asp Phe Ser Gly Pro 270 Ser Asn

Asp Leu Leu 275 <210> 200

<211> <212>

63 PRT

<213> Artificial sequence <220>

<223> DOF domain <400> 200

Pro Read Gly Wing Pro Read Lys Cys Pro Arg Cys 15 10

Lys Phe Cys Tyr Tyr Asn Asn Tyr Asn Leu Thr

20

25

Cys Lys Gly Cys Arg Arg Tyr Trp Thr Gln Gly

35

40

Val Pro Val Gly Gly Gly Cys Arg Arg Asn Asn

50 55

<210> 201 <211> 684 <212> DNA

<213> Arabidopsis thaliana <400> 201

atggcggaga gagcaaggca ggccaacatt cctccactag cgatgcgact ccagcaacac taagttctgt tactacaaca cgtcacttct gcaaaggttg ccgtcgctac tggacacaag cctgtaggtg gaggctgccg gaggaataac aagaagggca tcttcttctt cgtccaaaca gtcttcctcg gtcaacgctc ctaaggacaa atcatcagtt ccctttttca ccaactcttt ggtattggtt tgaacttagc cgctactaat ggcaacaacc agtttgatga tgagcgatct agggtttctc catggacgaa ggaaacattc atgaaaacaa caacaataat aacaatgaaa ggatctttga gcccctttgc tctcttcgat ccaacgacgg gacggtaata tcgggaacaa cgttgggata tctggttctt agggtttatc agacgctccg GTGA <210> 202 <211> 227 <212> PRT

<213> Arabidopsis thaliana <400> 202

Met Glu Wing Arg Wing Arg Gln Wing Asn Ile Pro 15 10

Read Lys Cys Pro Arg Cys Asp Being Ser Asn Thr

Asp Ser Ser Asn Thr 15

Gln Pro Arg His Phe 30

Gly Wing Read Arg Asn 45

Lys Lys Gly Lys 60

cgggacccct actataacct ggggcgccct aaaatggaaa aaagtcctag acaatctcac aagctcacca atacttcaac acaacctaat ggctatacgc ctacttccat

aaagtgtcct cactcagcct gagaaacgtc tttaaaatct ctcaggacag tcaactcgga gatcggttcc tccgatgacg ggcatccgtt tttccagaac ggttgattct

60 120 180 240 300 360 420 480 540 600 660 684

Pro Leu Wing Gly Pro 15

Lys Phe Cys Tyr Tyr 20 25

Asn Asn Tyr Asn Read Thr Gln Pro Arg His Phe

35 40

Arg Tyr Trp Thr Gln Gly Gly Wing Read Arg Asn

50 55

Gly Cys Arg Arg Asn Asn Lys Lys Gly Lys Asn 65 70 75

Ser Ser Ser Ser Ser Lys Gln Ser Ser Ser Val

85 90

Being Being Gly Gln Read Arg Thr Asn His Gln Phe

100 105

Leu Tyr Asn Leu Thr Gln Leu Gly Gly Ile Gly

115 120

Thr Asn Gly Asn Asn Gln Wing His Gln Ile Gly

130 135

Ser Asp Read Gly Phe Read His Gly Arg Asn Thr 145 150 155

Gly Asn Ile His Glu Asn Asn Asn Asn Asn Asn

165 170

Met Ala Ser Val Gly Ser Leu Ser Pro Phe Ala

180 185

Thr Gly Leu Tyr Ala Phe Gln Asn Asp Gly Asn

195 200

Gly Ile Be Gly Be Be Thr Be Met Val Asp

210 215

Thr Leu Arg 225

<210> 203 <211> 993 <212> DNA

<213> Arabidopsis thaliana <400> 203

atgcctacga attcgaatca tcagcatcat cttcaacacc ataataagtg gccacggact agtactctct caccaacttc aaccctaacc accaccatgt cgctacctct gctggtcttc atggcggaga gagcaaggca ggccaacatt cctccactag cgatgcgact ccagcaacac taagttctgt tactacaaca cgttacttct gcaaaggttg ccgtcgctac tggacacaag cctgtaggtg gaggctgccg gaggaataac aagaagggca tcttcttctt cgtccaaaca gtcttcctcg gtcaacgctc ctaaggacaa atcatcagtt ccctttttca ccaactcttt ggtattggtt tgaacttagc cgctactaat ggcaacaacc agtttgatga tgagcgatct agggtttctc catggacgaa ggaaacattc atgaaaacaa caacaataat aacaatgaaa ggatctttga gcccctttgc tctcttcggggggggggggggggggggggggg

<213> Arabidopsis thaliana <400> 204

Met Pro Thr Asn Ser Asn His Gln His His Leu 15 10

Glu Asn Gly Be Ile Ile Be Gly His Gly Leu

30 Cys Lys Gly Cys Arg 45 Val Pro Val Gly Gly 60 Gly Asn Leu Lys Ser 80 Asn Wing Gln Ser Pro 95 Phe Ser Pro Thr 110 Leu Asn Leu Wing Ala 125 Ser Ser Leu Met Met 140 Ser Thr Pro Met Thr 160 Asn Glu Asn Asn Leu 175 Leu Phe Asp Pro Thr 190

agcttaacga cacctctcca cgtcaaggat cgggacccct actataacct ggggcgccct aaaatggaaa aaagtcctag acaatctcac aagctcacca atacttcaac acaacctaat ggctatacgc ctacttccat ctaattccat

aaatggaagt agcaaaccct gggtggatcg aaagtgtcct cactcagcct gagaaacgtc tttaaaatct ctcaggacag tcaactcgga gatcggttcc tccgatgacg ggcatccgtt tttccagaac ggttgattct tggtg tactgg

20

25

Read Pro Pro Read Gln Wing Asn Pro Asn Pro Asn

35

40

Thr Be Gly Wing Read Pro Be Arg Met Gly Gly

50

55

Wing Arg Gln Wing Asn Ile Pro Pro Read Wing Gly

Gln His Gln Leu Asn 15

Val Leu Ser His Gln 30

His His His Val Wing 45

Be Met Wing Glu Arg 60

Pro Read Lys Cys Pro

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 993 65 70 75 80 Arg Cys Asp To Ser 85 Asn Thr Lys Phe Cys 90 105 Gly Cys Arg Arg Tyr 110 Trp Thr Gln Gly Gly 115 Wing Read Le Arg Asn Val 120 Pro Val Gly Gly 125 Cys Arg Arg Asn Asn 130 Lys Lys Gly Lys Asn 135 Gly Asn Leu Lys Be 140 Ser Be Ser Be Ser Lys Gln Ser Ser Ser Val Asn Ala Gln Ser Pro Ser Ser Gly Gln 145 150 155 160 Leu Arg Thr Asn His 165 Gln Phe Pro Phe Ser 170 Pro Thr Leu Tyr Asn 175 Leu Thr Gln Leu Gly 180 Gly Ile Gly Leu Asn 185 Leu Ala Wing Thr Asn 190 Gly Asn Asn Gln Wing 195 His Gln Ile Gly Be 200 Ser Leu Met Met Ser 205 Asp Leu Gly Phe Leu 210 His Gly Arg Asn Thr 215 Ser Thr Pro Met Thr 220 Gly Asn Asn Asn Asn Asn Asn Asn Asn Glu Asn Asn Leu Met Ala Ser Val 225 230 235 240 Gly Be Leu Ser Pro 245 Phe Ala Leu Phe Asp 250 Pro Thr Thr Gly Leu 255 Tyr Ala Phe Gln Asn 260 Asp Gly Asn Ile Gly 265 Asn Asn Val Gly Ile 270 Ser Gly Ser Ser Thr 275 Ser Met Val Asp Ser 280 Arg Val Tyr Gln Thr 285 Pro Val Lys Met 290 Glu Glu Gln Pro Asn 295 Leu Wing Asn Leu Ser 300 Arg Pro Val Ser Gly Leu Thr Be Pro Gly Asn Gln Thr Asn Gln Tyr Phe Trp Pro Gly 305 310 315 320 Be Asp Phe Be Gly 325 Pro Be Asn Asp Ile 330 Leu

<210> 205

<211> 873

<212> DNA

<213> Oryza sativa

<400> 205

atgattccgg cgatggcggc ggcggaggcg ccgatgtcga gcacgcttgc tgtcggagag ggcgcggctg gcgaggatcc aagtgcccgc gctgcgactc caccaacacc aagttctgct tcccagcccc gccacttctg ccgcgcctgc cgccgctact cgcaacgtcc ccgtcggcgg cggctaccgc cgccacgcca gcgtccgcgg cgggctccgc ctcagccgcc accaccaccg tcgacgacga cgacgacgac gtcctccacc tgcgccacgc gcgatgctgg gcggcaacct ctccatcctg ccgccgctgc gccatgagcc tcggctccac cttctctggc atggcggcgg gtcgacgcgg ccggctgcta ctccgtcggc gccgccaccg cagcagatgc agagcttccc gttcttccac gccatggatc ccaccgccgg caatggcaat gccggggatg ttccagctag ggcagcggcg gcggcgaaga cggtggagag ctccaccatg gaaggctacc caaggggcat gtatggcgat catcacctcg tccagtgcaa ccacaggtaa ccatctcttg taa <210> 206 <211> 290 <212> PRT

<213> Oryza sativa <400> 206

Met Ile Pro Gly Thr Read Wing Asp Gly Gly Gly 15 10

Gly Pro Wing Lys Pro Met Be Met Be Glu Arg

20 25

Ile Pro Read Pro Glu Pro Gly Read Lys Cys Pro

gcgcggtggg cgctgccgga acttcaacaa ggacccgcgg agcgcgccaa ccggctcgac ccaacgcgcc tccgcctcgc ccgccggcaa gcctcgagca accaggcggc gcctagacgg cgatgcgcata

gccggcgaag gccggggctc ctactccctc cggcgcgctc gcccaagccg gccagcgggg cgccctcccg cgacttcgac gccaccgccc atggagacta gatggcggcg cgacggccat atcgaagaga cact

60 120 180 240 300 360 420 480 540 600 660 720 780 840 873

Gly Gly Gly Wing Val 15

Arg Wing Leu Arg Wing 30

Arg Cys Asp Be Thr 35 40 45 Asn Thr Lys Phe Cys Tyr Phe Asn Asn Tyr Be Read Ser Gln Pro Arg 50 55 60 His Phe Cys Arg Wing Cys Arg Arg Tyr Trp Thr Gly Gly Wing Leu 65 70 75 80 Arg Asn Val Pro Val Gly Gly Gly Tyr Arg Arg His Wing Lys Arg Wing 85 90 95 Lys Pro Lys Pro Wing Be Wing Gly Be Wing Be Wing Wing Thr Thr 100 105 110 Thr Wing Gly Wing Thr Pro Wing Gly Wing Thr Thr Ser 115 120 125 Ser Thr Cys Ala Thr Pro Asn Ala Pro Ala Leu Pro Ala Met Leu Gly 130 135 140 Gly Asn Leu Ser Ile Leu Pro Pro Leu Arg Leu Asp Phe Asp 145 150 155 160 Alle Met Ser Leu Gly Ser Thr Phe Ser Gly Met Wing Ala Wing Gly Wing 165 170 175 Lys Pro Pro Pro Val Asp Wing Gly Cys Wing Tyr Be Val Gly Wing Wing 180 185 190 Thr Gly Leu Glu Gln Trp Arg Leu Gln Met Gln Ser Phe Pro Phe 195 200 205 Phe His Wing Met Asp His Gln Wing Met Wing Wing Pro Wing Pro Pro Wing 210 215 220 Met Wing Met Pro Gly Met Phe Gln Leu Gly Leu Asp Gly Asp Gly His

225 230 235

Gly Ser Gly Gly Gly Glu Asp Gly Gly Glu Leu

245 250

Being Being Lys Arg Glu Gly Tyr

260 265

Read Wing Gly Gly Tyr Thr Be Tyr Ser Be Wing 275 280

Leu Leu 290 <210> 207 <211> 1068 <212> DNA <213> Oryza sativa <400> 207

atgccgccgc atcacggcgg cctcatggcg cctcggcctg gcggcgagcg gcggcggcgg tggcggcggc ggcccgaccg ggctcgatga cggaacgggc tcggctggcg aagatcccgc tgcccgcgct gcgagtccac caacaccaag ttctgctact cagccgcgcc acttctgcaa gacgtgccgc cgctactgga aacgtccccg tcggcggcgg gtgccgccgc aacaagcgca tcgtcgacgt cggccgccgg ctcggcctcc gccaccggcg tcgaccgcca cgggtggcag cagcagcgcc gcggcggccg gcgcagctgc cgttcctggc ctcgttgcac cacccgctcg tccggtgcgt ccaggctagg gtttcccgga ttgagctcgc ctcggcggcg gcgccgccgc cgccgccgcc atcgggctag atacagcaat tccccttctt gagccgcaac gacgccatgc tacccgttcg acgcggaggc cgccgccgac gccgccggct ggcaccaagg tgcccggctc gtcgggcctg atcacgcagc gacagcaacg ctcagtccgc ggcgatgaac agctcgccga ggcaacctcc aattctgggg cggtggcaac ggcgcgggac gccaccggcg gcagcggcgc cggtgtcgct ccgggaggcg gctgatctct ccggattcaa ctcgtcgtcg tcggggaaca <210> 208 <211> 355 <212> PRT

<213> Oryza sativa <400> 208

Met Pro Pro His Gly Gly Leu Met Pro Wing 15 10

His his

Tyr gly

Thr Thr 285

Ala Met 255 Asp His 270

Gly asn

240 Pro

His

His

acgggtagc gcggcacggc agccggagcc tcaacaacta cgcgcggcgg ccaagtcgtc gcacgtcgtc cggcgatgat gcggcggcga tggatcccgt aggaggggggggggcggggggggggggggggggggggggggg

agcggccgtc ggtgcggccg ggggctcaag ctcgctctcg agcgctccgc caagtcgtcc gtccacatcg gccgccgcag tcactacagc cgactaccag cctcccgcag gtccggcgggggggggggggggggggggg

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1068

Arg Pro Asp Met Val 15 Ala Wing Ala Val Wing Ala Wing Be Gly Gly Gly Gly Gly Gly Pro 20 25 30 Thr Gly Gly Thr Val Val Pro Gly Be Met Thr Glu Arg Wing Arg 35 40 45 Leu Wing Lys Ile Pro Gln Pro Glu Pro Gly Read Lys Cys Pro Arg Cys 50 55 60 Glu Be Thr Asn Thr Lys Phe Cys Tyr Phe Asn Tyr Be Read Le Ser 65 70 75 80 Gln Pro Arg His Phe Cys Lys Thr Cys Arg Tyr Trp Thr Arg Gly 85 90 95 Gly Wing Read Le Arg Asn Val Pro Val Gly Gly Gly Cys Arg Arg Asn Lys 100 105 110 Arg Thr Lys Be Be Lys Be Be Be Thr Be Wing Gly Wing 115 115 125 Wing Be Wing Thr Gly Gly Thr Be Be Thr Be Be Thr Thr Wing 130 135 140 Gly Gly Be Be Be Wing Wing Wing Wing Wing Wing Wing Met Met Pro Pro Gln 145 150 155 160 Wing Gln Leu Pro Phe Leu Wing Be His His Pro Leu Gly Gly Gly 165 170 175 Asp His Tyr Ser Ser Gly Al Ser Ser Arg Read Gly Phe Pro Gly Ser Ser 180 180 190 Ser Leu Asp Pro Val Asp Tyr Gln Leu Gly Gly Gly Wing Ala Wing Ala Wing 195 200 205 Ala Wing Ile Gly Leu Glu Gln Trp Arg Leu Pro Gln Ile Gln Phe 210 215 220 Pro Phe Leu Ser Arg Asn Asp Ala Met Pro Pro Met Ser Gly Ile 225 230 235 240 Tyr Pro Phe Asp Wing Glu Wing Wing Wing Asp Wing Gly Wing Phe Wing Gly 245 250 255 Gln Leu Leu Wing Gly Thr Lys Val Pro Gly Being Ser Gly Leu Ile Thr 260 265 270 Gln Leu Wing Ser Val Lys Met Glu Asp To Be Asn Wing Gln To Be Wing 275 280 285 Met Asn To Be Pro Arg Glu Phe Leu Gly Leu Pro Gly Asn Leu Gln 290 295 300 Phe Trp Gly Gly Gly Asn Gly Wing Gly Asn Gly Asp Gly 305 310 315 320 Wing Thr Gly Gly Be Gly Wing Gly Val Val Wing Pro Gly Gly Gly Gly Ser 325 325 335 Gly Gly Gly Gly Trp Wing Asp Read Gly Phe Asn Be Ser Be Ser Gly

340 345 350

Asn Ile Leu 355 <210> 209 <211> 1000 <212> DNA

<213> Triticum aestivum <400> 209

ctcctagctc gtcgacaagc atgcccacaa agccactgat aacaagcgca tagcagcgca 60

cgctctctta tagtagaatg ttctgaactc cacaccagcc catgcaagac ttccagtcca 120

tcccgggcct cgccgggcgg ctgttcggcg gcgcggccgc ggcagacatc cggcgcgtgc 180

agggcccggc gtcccggtgc ggcgtgttct cgcaggcggc gtccgcgcag ccggaggcgg 240

ccgtcaagtg cccgcggtgc gagtccacca acaccaagtt ctgctactac aacaactaca 300

acctgtcgca gccgcgccac ttctgcaaga gctgccgccg gtactggacc aagggcggcg 360

tcctccgcaa cgtccccgtc ggcggcggct gccgcaaggc caagcgcagc tcctcgtcgg 420

cgtccgcacc gtcgacgccc gcggccacgg acgccaagag ccagcggcgc gcgtccgcgt 480

cgtcctcctc ccgctccaac agcggcagcg gcagcgccag ccccacggcc gctgcggaag 540

agacgacgac aacggagacc gagccccctc ctccgcccac gccgtcgtcc aactccaact 600

ccaacgcggt ctccttcgcc aaccgcatga cgaactaccc cttcgcggca gacgtgccac 660

ctctggcgcc gatattcgcc gaccaggccg ccgcgctcgc gtccctcttc gcgccgcctc 720

ctccaccgcc tctcccggtg ttcagcttct cggcggagcc caagatggag gaggcgatcg 780

ggtcactgct gctcccgggg caggaggcgt cgcaggagcc agaggagccc acctgcacct 840 ccaccgtcgc ggacatggcc ccgttcatgt cgctggacgc ggggatcttc gagctcggcg 900

acgcgtcgcc ggccgattac tggaacggcg ggagctgctg gacggacgtc caggacccgt 960

ccgtctacct accctagttt ggcttagttc ctcatcacga 1000 <210> 210

<211> 291 <212> PRT <213> Triticum, aestivum <400> 210 Met Gln Asp Phe Gln Being Ile Pro Gly Leu Wing Gly Arg Leu Phe Gly 1 5 10 15 Gly Wing Wing Wing Asp Ile Arg Arg Val Gln Gly Pro Wing Be Arg 20 25 30 Cys Gly Val Phe Be Gln Wing Wing Be Wing Gln Pro Glu Wing Wing Val 35 40 45 Lys Cys Pro Wing Wing Arg Thr Cys Tyr Tyr Asn 50 55 60 Asn Tyr Asn Leu Ser Gln Pro Arg His Phe Cys Lys Be Cys Arg 65 65 75 80 Tyr Trp Thr Lys Gly Gly Val Leu Arg Asn Val Pro Val Gly Gly Gly 85 90 95 Cys Arg Lys Ala Lys Arg Be Ser Be Ala Ser Ala Pro Be Thr 100 105 110 Pro Wing Wing Thr Asp Wing Lys Be Gln Arg Arg Wing Be Wing Be Ser 115 115 125 Be Wing Arg Be Asn Wing Gly Be Gly Wing Wing Be Pro Thr Wing Wing 130 135 140 Wing Glu Wing Glu Thr Wing Wing Glu Pro Pro Pro Pro Pro 145 150 155 160 Pro Ser Ser Asn Ser Asn Ser Asn Ala Val Wing Ser Phe Ala Asn Arg Met 165 170 175 Thr Asn Tyr Pro Ala Asp Val Pro Ala Wing Ala Pro Ala Ile Phe 180 185 190 Ala Asp Gln Ala Ala Leu Ala Ala Phe Ala Pro Pro Pro Pro 195 200 205 Pro Pro Leu Pro Val Phe Ser Phe Ser Glu Wing Pro Lys Met Glu Glu 210 215 220 Wing Ile Gly Ser Leu Leu Pro Gly Gln Glu Wing Ser Gln Glu Pro 225 230 235 240 Glu Glu Pro Thr Cys Thr Ser Thr Val Wing Asp Met Pro Wing Phe Met 245 250 255 Ser Leu Asp Wing Gly Ile Phe Glu Leu Gly Asp Wing Ser Pro Wing Asp 260 265 270 Tyr Trp Asn Gly Gly Ser Cys Trp Thr Asp Val Gln Asp Pro Ser Val 275 280 285 Tyr Leu Pro 290

<210> 211 <211> 1230 <212> DNA <213> Zea mays <400> 211

aggtggtggc gggggacagg ttgggggccc cgccaagccc atgtccatgg cggagcgcgc 60

gcgcctcgcg aggatcccac tgccggagcc gggactcaag tgcccgcgct gcgactcaac 120

caacaccaag ttctgctact tcaacaacta ctccctcacg cagccgcgcc acttctgccg 180

ggcctgccgc cgctactgga cgcgtggcgg cgcgctccgc aacgtgccgg tcggtggcgg 240

ataccgccgc cacgccaagc gcgccaagcc caagcagcag cagcagcacg cggccgccgg 300

gaccggagct gccaacggcg cgatgcagca gcctcccgct gggtctatgg cgtcgtcggc 360

cgccgcctgc accgcgacca cgacgatgac gaccaacgcg ctggacgccg ggcccggcgg 420

catgctgccc atgctgccgc cgctcgtccg cctagcagac ttcgacgcaa tgagcctcgg 480

ctccagcttc tccgggatat cgtccatggg gaagcccgga tccatcggcg cggcctgcta 540

cccgcactct gtcggcgggc tggagcagtg gagggtgcag cagatgcaga gcttcccgtt 600

cttgcatgcg atggaccagg gcccgctggg gccacctctg gccatggcga tggcggcgcc 660

aggagggatg ttccagctag gtctagacac caccagtgat aacagccgtg gcggtggcgg 720 cggcggctgc ggcgaagacg ggtcgtctgc gggagaggcg caccaagagg gagagctacc cggcaccacc aagagccatg tcacctcgct gctgctggtg gctacacttc ctattccacc tctcttgtaa tggccggccg atcgatcgat cgagagctca tagctagcta ctacgacgtc gtgctacgat cgtatcggtt tagcctagag atagagtgtc tctgtctgtg tgatcgatgg aaagaccagt gtagcatgca tgcacttgtt gcaatgtttg ggaggagagg ctcggtttgg atgctgatca tgcacaaata tacttcatta taaaaaaaaa aaaaaaaaaa <210> 212 <211> 302 <212> PRT <213> Zea mays <400> 212

Gly Gly Gly Gly Gly Gly Gln Val Gly Gly Pro Wing 15 10

Glu Wing Arg Wing Arg Leu Arg Wing Arg Ile Pro Leu

20 25

Lys Cys Pro Arg Cys Asp Being Thr Asn Thr Lys

35 40

Asn Tyr Being Read Thr Gln Pro Arg His Phe Cys

50 55

Tyr Trp Thr Arg Gly Gly Wing Read Arg Asn Val 65 70 75

Tyr Arg Arg His Wing Lys Arg Wing Lys Pro Lys

85 90

Wing Wing Wing Wing Gly Thr Wing Wing Wing Wing Wing Wing Gly Wing

100 105

Wing Gly Be Met Wing Be Being Wing Wing Cys Wing

115 120

Met Thr Thr Asn Wing Read Asp Wing Gly Pro Gly

130 135

Leu Pro Pro Leu Val Arg Leu Wing Asp Phe Asp 145 150 155

Be Ser Phe Ser Gly Ile Ser Ser Met Gly Lys

165 170

Wing Cys Wing Tyr Pro His Ser Val Gly Gly Leu

180 185

Gln Gln Met Gln Be Phe Pro Phe Read His Wing

195 200

Read Gly Pro Pro Read Met Wing Met Wing Met Wing 210 210

ctccatatga tacggcgacc aatgctgctg acaattcaag tcggttcgtt tattgttatg ctttcaagaa ttactagtgt

tgcaagcagc aacaccacaa caggtaacca tgtcctgcta cctacaaata atcatataga gaaactggag ctacagctgc

Gln Leu Gly Leu Asp Thr Thr Be Asp Asn Ser 225 230 235 Gly Gly Cys Gly Glu Asp Gly Be Ser Wing Gly 245 250 Met Gln Wing Wing Thr Lys Arg Glu Ser Tyr Pro 260 265 Met Tyr Gly Asp Allah

275

280

Thr Be Tyr Be Thr Asn Wing Wing Wing Gly Wing

290 <210> 213 <211> <212>

295

Lys Pro Met Be Met 15 Pro Glu Pro Gly Leu 30 Phe Cys Tyr Phe Asn 45 Arg Wing Cys Arg Arg 60 Pro Val Gly Gly 80 Gln Gln Gln His 95 Met Gln Gln Pro Pro 110 Thr Wing Thr Thr Thr 125 Gly Met Leu Pro Met 140 Wing Met Ser Leu Gly 160 Pro Gly Ser Ile Gly 175 Glu Gln Trp Arg Val 190 Met Asp Gln Gly Pro 205 Pro Gly Gly Met Phe 220 Arg Gly Gly Gly Gly Gly 240 Glu Wing Read His Met 255 Ala Pro Pro Arg Wing 270 Wing Wing Gly Gly Tyr 285 His Leu Leu 300

1243 DNA

<213> Solanum tuberosum <400> 213

gaggacttat caaccttttt atataaaaga gataaagatc atcatggttt tctcatcttt tcctgtgtat ctagatcatc cagccagacg gccatcaaca aggaaatact gggctggaga cctatgcagg tgggggctag tccgggctca atcagaccag cggttagcta agattccact accggaagct ggattaaagt aacacaaagt tctgctactt caacaactac aacctttccc

aaagagatca ccaatttgca atccaactct gttctatggt gtccaaggtg aaccaagaca

aagaaagaaa tcagttacaa gcagccccca ggatcgagcc tgattcaaca cttctgcaag

780 840 900 960 1020 1080 1140 1200 1230

60 120 180 240 300 360 acctgtcgcc ggtactggac tagaggggggc gccttgagaa gcgtgccggt aggaggagga 420

tgccggagga acaagagaag caaaagtaat aacaacaaca acagctcaaa gacagctggc 4 80

agtaatgtca atactaatac tattgcttcc ggtacttcaa caagtgcaag tccttcaagc 540

tgcagcacgg aaataatgaa tggtcgccat cacttctctc atgagcaacc accgcagtta 600

actcctctca tggctgcttt ccaaaatctt aatcatcact atggcggatt tcaacctcct 660

cctttggttt caacacacca tgggaatgga accggcgcgc ttggtcatca tcatgaaatg 720

ggatttcaaa tagggagtag tactaatact aacaatttac ctgtaccccc aggaggagga 780

tctgatcatc agtggagatt accatctttg gcagcaaaca caaatttgta cccttttcaa 840

cacggtactg atcaaggaat tcatgaatca tcatctgtta acaataataa tattaatgct 900

catgatgacc aagggttaaa ttcgaccaaa cagtttttgg gaacaatgga aaataatact 960

aatcaatatt ggggtggaaa cgcatggaca gggttttctg gattaaactc atcttcttca 1020

gcaagccatc tactttgatt tcattcatgt aagtcaattt gtgtacttga ctctcaacga 1080

ttaaaatgtt gtatgtgttg ggaggggggt ctaaattaga tatatagtat actgggggca 1140

tgtttaagtc tttgttattc catggtcatt atcaccactt gtaattttac tggatgtttt 1200

ttttttataa cttaggtgtg tgaagttgta ttgtgagttt taa 1243 <210> 214 <211> 324 <212> PRT

<213> Solanum tuberosum <400> 214

Met Val Phe Be Ser Phe Pro Val Tyr Leu Asp His Pro Asn Leu His 1 5 10 15 Gln Leu Gln Gln Pro Asp Gly His Gln Gly Asn Thr Gly Leu Glu 20 25 30 Asn Pro Thr Leu Gln Pro Pro Met Gln Val Gly Wing Be Pro Gly 35 40 45 Be Ile Arg Pro Gly Be Met Val Asp Arg Wing Arg Leu Wing Lys Ile 50 55 60 Pro Leu Pro Glu Wing Gly Leu Lys Cys Pro Arg Cys Asp Ser Thr Asn 65 70 75 80 Thr Lys Phe Cys Tyr Phe Asn Asn Tyr Asn Leu Be Gln Pro Arg His 85 90 95 Phe Cys Lys Thr Cys Arg Arg Tyr Trp Thr Gly Gly Wing Leu Arg 100 105 110 Ser Val Pro Val Gly Gly Gly Cys Arg Asn Lys Arg Be Lys Ser 115 120 125 Asn Asn Asn Asn Asn Being Ser Lys Thr Wing Gly Being Asn Val Asn Thr 130 135 140 Asn Thr Ile Wing Being Gly Thr Being Thr Being Wing Being Pro Being Cys 145 150 155 160 Being Glu Ile Met Thr Asn Gly Arg His His Phe Be His Glu Gln Pro 165 170 175 Pro Gln Leu Thr Pro Leu Met Wing Al a Phe Gln Asn Leu Asn His His 180 185 190 Tyr Gly Gly Phe Gln Pro Pro Pro Leu Val Ser Thr His His Gly Asn 195 200 205 Gly Thr Gly Wing Leu Gly His His Glu Met Gly Phe Gln Ile Gly 210 215 220 Ser Ser Thr Asn Thr Asn Asn Leu Pro Val Pro Gly Gly Gly Ser 225 230 235 240 Asp His Gln Trp Arg Leu Pro Ser Leu Wing Asn Thr Asn Leu Tyr 245 250 255 Pro Phe Gln His Gly Thr Asp Gln Gly Ile His Glu Ser Ser Ser Val 260 265 270 Asn Asn Asn Asn Ile Asn Wing His Asp Asp Gln Gly Leu Asn Ser Thr 275 280 285 Lys Gln Phe Leu Gly Thr Met Glu Asn Asn Thr Asn Gln Tyr Trp Gly 290 295 300 Gly Asn Wing Trp Thr Gly Phe Ser Gly Leu Asn Ser Ser Ser Ala

305

To be his leu leu

310

315

320

<210> 215 <211> 996 <212> PRT

<213> Arabidopsis thaliana <400> 215

Wing Thr Gly Cys Cys Thr Wing Cys 1 5 Wing Thr Cys Wing Thr Thr Cys Wing Gly 20 Thr Cys Wing Wing Cys Wing Cys Cys 35 40 Gly Wing Wing Wing Thr Gly Gly 50 55 Wing Wing Gly Thr Gly Cys 65 70 Wing Gly Thr Cys Wing Cys Thr Cys Thr 85 Cys Thr Thr Cys Cys Wing Cys Cys 100 Cys Wing Cys Wing Cys Cys Thr 115 120 Cys Cys Wing Cys Cys Thr 130 130 Cys Wing Cys Thr Cys Thr Gly Cys 145 150 Cys Gly Thr Cys Wing Gly Wing Gly 165 Wing Thr Cys Gly Wing Gly Thr Thr Wing Gly 180 Gly Cys Wing Gly Wing Gly Cys Wing 195 200 Thr Thr Cys Wing Cys Cys Wing 210 215 Wing Cys Cys Wing Cys Thr Wing Wing 225 230 Cys Gly Wing Wing Thr Gly Cys Gly Ala 245 Cys Ala Cys Ala Cys Thr Ala Ala Gly 260 Cys Ala Cys Ala Cys Ala 275 280 Cys Ala Cys Ala Cys Thr Ala 290 295 Ala Cys Ala Cys Thr Ala Cly 305 310 Cys Ala Cys Gly Ala Cys Thr 325 Cys Wing Gly Wing Gly Gly Gly Gly 340 Gly Wing Cly Wing Cys Gly Thr Cys 355 360 Thr Wing Gly Gly Wing Gly Cly Thr 370 375 Wing Wing Thr Wing Wing Cys Wing Wing 385 390 Wing Wing Wing Wing Thr Wing Gly Gly Wing 405 Wing Wing Thr Cys Thr Wing 420 Thr Cys Wing Wing Wing Cys Wing Wing 435 440 Cys Gly Gly Thr Cys Wing Cys Wing 450 455 Thr Cys Cys Thr Wing Gly Cys Thr

117

Gly Wing Ward Thr Thr Cys Gly Wing 10 15 Cys Wing Thr Cys Wing Thr Cys Thr 25 30 Wing Gly Cys Wing Thr Thr Wing Cys 45 Wing Gly Thr Wing Wing Thr 60 Wing Cys Gly Gly Wing Wing Cys Thr 75 80 Cys Thr Cys Wing Cys Cys Wing Cys Wing 90 95 Cys Thr Wing Cys Cys Wing Gly Wing 105 110 Wing Cys Wing Cys Cys Wing Wing 125 Wing Gly Thr Wing Cys Gly Cys 140 Wing Gys Thr Thr Cly 155 Wing Wing Gys Thr Gly Gly Gly Thr Gly Gly 170 175 Cys Gly Gly Wing Gly Wing Gly Wing 185 190 Gly Gly Cys Wing Cys Wing 205 Cys Thr Wing Gly Cys Gly Gly 220 Wing Gly Thr Gly Thr Cys Thr 235 240 Cys Thr Cys Cys Wing Gly Cys Wing 250 255 Thr Thr Cys Thr Gly Thr Thr Wing 265 270 Wing Cys Thr Wing Thr Thr Wing Cys 285 Gly Cys Cys Thr Cys Gly Thr Cys 300 Wing Wing Gly Gly Thr Thr Gly 315 320 Wing Cys Thr Gly Gly Wing Cys Wing 330 335 Cys Gly Cys Cys Cys Thr Gly Wing 345 350 Cys Cys Thr Gly Th r Wing Gly Gly 365 Gly Cys Cys Gly Gly Wing Gly Gly 380 380 Gly Wing Gly Gly Gly Cys Wing 395 400 Wing Wing Thr Thr Thr Wing Wing 410 415 Cys Thr Thr Cys Thr Thr Cys 425 430 Gly Thr Cys Thr Thr Cys Cys Thr 445 Gly Cys Thr Cys Wing Gly Wing 460 Cys Wing Gly Gly Wing Cys Wing Gly 465 470 Cys Thr Wing Gly Wing Gly Cys 485 Wing Gly Thr Thr Cys Cys Thr 500 Wing Cys Thr Wing Cys Thr Thr Thr 515 520 Wing Cys Thr Cys Wing Wing Cys Thr Wing 530 535 Thr Thr Gly Gly Wing Thr Thr Gly 545 550 Cys Gly Wing Cys Thr Wing Wing 565 Wing Cys Wing Cys Wing Wing Gly Cys Wing 580 Thr Cys Gly Wing Wings Thr Cys 595 600 Gly Wing Wing Thr Gly Wing Gly Cys Gly 610 615 Thr Thr Thr Cys Thr Cys Cys Wing 625 630 Wing Thr Thr Cys Wing Thr Thr Cys Wing 645 Gly Wing Cys Gly Gly Wing 660 Gly Wing Wing Cys Wing Wing 675 680 Wing Thr Wing Wing Cys Wing Ala Thr 690 695 Cys Wing Ala Thr Gly Gly 705 710 Gly Gly Wing Thr Thr Cys Thr Thr 725 Thr Thr Gly Cys Thr Thr Cys 740 Wing Gly Cys Wing Thr Cys Gly 755 760 Gly Cys Wing Thr Thr Cys Wing 770 775 Gly Wing Wing Thr Thr Cys 785 790 Cys Gly Thr Thr Gly Gly Wing 805 Thr Cys Thr Thr Cys Thr Wing Cys 820 Thr Thr Gly Wing Thr Thr Cys Thr 835 840 Thr Cys Wing Gly Wing Cys Gly Cys 850 855 Wing Gly Wing Thr Gly Gly Wing 865 870 Cys Thr Wing Thr Thr Wing Gly 885 Gly Thr Cys Thr Wing Gly Wing Cys 900 Gly Wing Thr Thr Thr Gly Wing Cys 915 920 Gly Wing Wing Thr Thr Cys Wing 930 935 Gly Wing Wing Cys Thr Thr Thr Wing 945 950 Thr Cys Gly Gly Wing Wing Thr Thr Thr

965

475 480 Wing Thr Cys Wing Thr Cys Wing 490 495 Thr Thr Thr Cys Wing Cys Cys 505 510 Wing Cys Wing Cys Thr Thr Wing 525 Cys Gly Gly Wing Gly Gly Thr Wing 540 Wing Cys Wing Thr Thr Wing Gly Cys 555 560 Wing Gly Gly Cys Wing Wing Cys Wing 570 575 Thr Cys Wing Cys Cys Wing Wing Gly Wing 585 590 Wing Gly Wing Thr Thr Gly Wing Wing 605 Wing Thr Thr Cys Wing Wing Gly Gly Wing 620 Wing Gly Gly Wing Cys Gly Wing Wing 635 640 Wing Cys Wing Thr Cys Cly Wing Gly Wing Thr 650 655 Cys Wing Wing Thr Thr Cys Wing Wing Thr 665 670 Cys Wing Cys Wing Wing Thr Thr Wing 685 Gly Wing Wing Cys Wing Wing 700 Cys Wing Thr Cys Wing Cly Gly Thr Thr 715 720 Gly Wing Gly Cys Wing Cys Cys Cys Thr 730 735 Thr Cys Thr Gly Wing Cys Cys Thr 745 750 Gly Gly Cys Thr Wing Thr Cys 765 Gly Wing Cys Gly Wing Cys Gly 780 Gly Gly Wing Cys Wing 795 800 Thr Thr Wing Cys Thr Gly Gly Thr 810 815 Thr Thr Cys Cys Wing Thr Gly Gly 825 830 Wing Gly Gly Gly Thr Thr Wing Wing 845 Cys Thr Cys Wing Cys Gly Gly Thr Wing 860 Gly Wing Wing Cys Wing Wing Cys Wing 875 880 Gly Cys Wing Wing Cys Thr Wing Wing 890 895 Cys Gly Gly Wing Wing Cys 905 910 Gly Thr Wing Cys Thr Cys Cys Thr Gly 925 Wing Cys Ward Wing Cys Thr Wing 940 Gly Gly Cys Wing Cys Thr Gly Gly 955 960 Cys Thr Cys Gly Gly Thr 970 975 Cys Thr Thr Cys Wing Gly Thr Cys Thr Wing Thr Thr 980 985 990 Gly Thr Gly Wing 995 <210> 216 <211> 275 <212> PRT <213> Arabidopsis thaliana <400> 216 Met Gly Gly Be Met Wing Glu Arg Wing Arg Gln Wing Asn Ile Pro Pro 1 5 10 15 Leu Wing Gly Pro Leu Lys Cys Pro Arg Cys Asp Being Ser Asn Thr Lys 20 25 30 Phe Tys Tyr Asn Asn Tyr Asn Leu Thr Gln Pro His Phe Cys 35 40 45 Lys Gly Cys Arg Tyr Trp Thr Gln Gly Gly Wing Read Arg A sn Val 50 55 60 Pro Val Gly Gly Gly Cys Arg Arg Asn Asn Lys Lys Gly Lys Asn Gly 65 70 75 80 Asn Leu Lys Be Ser Ser Ser Ser Ser Lys Gln Ser Ser Ser Val Asn 85 90 95 Gly Gln Leu Arg Thr Asn His Gln Phe Pro 100 105 110 Phe Ser Pro Thr Leu Tyr Asn Leu Thr Gln Leu Gly Ile Gly Leu 115 120 125 Asn Leu Wing Ala Thr Asn Gly Asn Gln Wing His Gln Ile Gly Ser 130 135 140 Be Read Met Met Be Asp Read Gly Phe Read His Gly Arg Asn Thr Be 145 150 155 160 Thr Pro Met Thr Gly Asn Ile His Glu Asn Asn Asn Asn Asn Asn Asn 165 170 175 Glu Asn Asn Leu Met Ala Be Val Gly Ser Leu Ser Pro Phe Wing Leu 180 185 190 Phe Asp Pro Thr Thr Gly Gln Thr Pro Pro Val Lys Met Glu Glu Pro Gln Asn Leu 225 230 2 35 240 Wing Asn Leu Be Arg Pro Val Be Gly Leu Thr Be Pro Gly Asn Gln 245 250 255 Thr Asn Gln Tyr Phe Trp Pro Gly Be Asp Phe Be Gly Pro Ser Asn 260 265 270 Asp Leu Leu

275

<210> 217 <211> 993 <212> DNA

<213> Arabidopsis thaliana <400> 217

atggttttct cctccatcca agcctatctt gattcatcca actggcaaca ggctcctccg 60

agcaattata atcatgacgg aacaggcgcc tcagcaaatg gaggtcatgt tcttcgtcct 120

cagctgcagc cacagcagca gccacagcag cagccgcatc ctaatgggag cgggggcgga 180

ggtggaggtg gaggcggctc gatccgagca ggatcaatgg tggacagagc aagacaagca 240

aacgtagcct tgccagaagc agcactgaaa tgtccgagat gcgaatccac caacaccaag 300

ttctgctact tcaacaacta cagcctcact caaccacgcc acttctgcaa gacctgccgg 360

agatactgga cacgtggcgg agctctccgc aacgtcccag tcggcggtgg ctgccggaga 420

aacaggcgta ccaaaagcaa cagcaacaac aacaataaca gcactgctac tagcaataac 480

accagtttct cctccgggaa tgcatccacc atcagcacga ttctctcctc ccactatgga 540

ggaaaccaag agagtatctt aagccagatt ttgtctccgg cgaggctaat gaatcctact 600

tacaatcatc tcggagatct cacaagtaat acaaaaacag acaacaacat gagcttgttg 660

aactatggag gattgagtca agacttgagg tcaatccaca tgggagcttc tggtggctcg 720

cttatgagct gtgttgatga atggagatcg gcgtcttatc atcagcagtc aagtatgggc 780 ggtgggaact tggaggattc ttctaatcct aatccatccg caaatgggtt ttactctttt 840 gagtcgccga ggataacttc agcgtcaatc tcttctgctt tagcatcgca gttttcttcg 900 gttaaagttg aagataatcc ttacaaatgg gttaatgtca atggtaattg ctcttcctgg 960 tga 993 aatgatcttt ctgctttcgg ctcttctcgt

<210> 218 <211> 330 <212> PRT

<213> Arabidopsis thaliana <400> 218

Met Val Phe Ser Be Ile Gln Wing Tyr Leu Asp Ser As Asn Trp Gln 1 5 10 15 Gln Wing Pro Pro As As Tyr Asn His Asp Gly Thr Wing Ally Ser Wing 20 25 30 Asn Gly Gly His Val Leu Arg Pro Gln Leu Gln Pro Gln Gln Gln Pro 35 40 45 Gln Gln Gln Pro His Pro Asn Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 50 50 60 60 Gly Gly Be Ile Arg Wing Gly Be Met Val Wing Asp Arg Wing Aln 65 70 75 80 Asn Val Wing Leu Pro Glu Ala Wing Leu Lys Cys Pro Arg Cys Glu Ser 85 90 95 Thr Asn Thr Lys Phe Cys Tyr Phe Asn Tyr Be Leu Thr Gln Pro 100 105 110 Arg His Phe Cys Lys Thr Cys Arg Tyr Trp Thr Gly Gly Wing 115 120 125 Leu Arg Asn Val Pro Val Gly Gly Gly Cys Arg Arg Asn Arg Arg Thr 130 135 140 Lys Be Asn Be Asn Asn Asn Asn As Thr Be Wing Ala Thr Be Asn Asn 145 150 155 160 Thr Be Phe Be Be Gly Asn Wing Be Thr Ile Be Thr Ile Read Be 165 170 175 Be His Tyr Gly Gly Asn Gln Glu Se r Ile Leu Be Gln Ile Leu Be 180 185 190 Pro Ala Arg Leu Met Asn Pro Thr Tyr Asn His Leu Gly Asp Leu Thr 195 200 205 Ser Asn Thr Lys Thr Asp Asn Met Ser Leu Asn Tyr Gly Gly 210 215 220 Leu Be Gln Asp Leu Arg Be Ile His Met Gly Ala Be Gly Gly Ser 225 230 235 240 Leu Met Ser Cys Val Asp Glu Trp Arg Be Ala Ser Tyr His Gln Gln 245 250 255 Be Ser Met Gly Gly Gly Asn Leu Glu Asp Ser Ser Asn Pro Asn Pro 260 265 270 Ser Asa Asn Gly Phe Tyr Ser Phe Glu Ser Pro Arg Ile Thr Ser Ala 275 280 285 Ser Ile Ser Ser Ala Leu Wing Ser Gln Phe Ser Ser Val Lys Val Glu 290 295 300 Asp Asn Pro Tyr Lys Trp Val Asn Val Asn Gly Asn Cys Be Ser Trp 305 310 315 320 Asn Asp Read Be Ala Phe Gly Be Ser Arg 325 330

<210> 219 <211> 972 <212> DNA

<213> Arabidopsis thaliana <400> 219

atggttttct catctcttcc agtgaatcag ttcgattccc aaaattggca gcagcaaggg 60

aaccaacatc agctagaatg tgtcacaact gaccagaacc ctaataatta cttacggcag 120

ctctcatcac caccgacttc tcaggttgca ggttcgagtc aagctagagt gaattcaatg 180

gtggaacgtg ctcggatcgc aaaagtccca ttgcctgaag cagctctaaa ttgccctaga 240

tgtgactcaa ccaatactaa gttctgttac ttcaataact atagccttac tcaacctcgc 300

catttctgca aaacatgtcg tcgctattgg acacgtggcg gttccttgag gaatgttcct 360

gttggaggag gctttaggag gaacaagaga agcaaatcca gatcgaaatc tacggtcgtg 420 gtctcgactg ataatactac tagtacttca tcacttactt ctcgcccaag ttactcaaac 480

cctagcaagt ttcatagcta cggtcaaatc ccggagttta attccaactt gcccatcttg 540

cctcctctcc aaagccttgg agattacaat tcaagcaaca ctggattaga ttttggtgga 600

actcaaataa gcaacatgat aagtggtatg agttctagtg gtgggatctt ggatgcatgg 660

agaatacctc catcacaaca agctcagcaa ttccctttct tgatcaacac taccggattg 720

gtgcaatctt caaacgcgtt atatccatta ctagaaggcg gggttagcgc cacgcaaaca 780

agaaatgtga aggcggaaga gaatgatcag gatcggggta gggatgggga tggagtgaat 840

aacttatcaa gaaacttttt gggtaatatc aacataaact caggcaggaa cgaggaatac 900

acatcatggg gaggtaacag ttcttggacc ggtttcacct ccaacaactc aacaggccat 960

ctctcattct aa 972 <210> 220 <211> 323 <212> PRT

<213> Arabidopsis thaliana <400> 220

Met Val Phe Ser Serenu Pro Val Asn Gln Phe Ser Serenator Asp Trn 1 5 10 15 Gln Serenator Gln Gly Asn Serenier Gln His Gln Serenator Valu Thr Thr Asp Serenator 25 25 30 Asn Serenator Asn Tyr Leu Serenator Seren Pro Pro Thr Be Gln 35 40 45 Val Wing Gly Be Ser Gln Wing Arg Val Asn Be Met Val Glu Arg Wing 50 55 60 Arg Ile Wing Lys Val Pro Leu Pro Glu Wing Wing Read Asn Cys Pro Arg 65 70 75 80 Cys Asp Ser Thr Asn Thr Lys Phe Cys Tyr Phe Asn Asn Tyr Ser Leu 85 90 95 Thr Gln Pro Arg His Phe Cys Lys Thr Cys Arg Arg Tyr Trp Thr 100 105 110 Gly Gly Ser Leu Arg Asn Val Pro Val Gly Gly Ply Arg Arg Asn 115 120 125 Lys Arg Be Lys Be Arg Be Lys Be Thr Val Val Val Be Thr Asp 130 135 140 Asn Thr Thr Be Thr Be Read Read Thr Be Arg Pro Be Tyr Asn 145 150 155 160 Pro Be Lys Phe His Be Tyr Gly Gln Ile Pro Glu Phe Asn Ser Asn 165 170 175 Leu Pro Ile Leu Pro Pro Leu Gln Se r Read Gly Asp Tyr Asn Be Ser 180 185 190 Asn Thr Gly Read Asp Phe Gly Gly Thr Gln Ile Be Asn Met Ile Ser 195 200 205 Gly Met Be Ser Gly Gly Ile Read Asp Wing Trp Arg Ile Pro 210 210 220 Ser Gln Gln Wing Gln Gln Phe Pro Phe Leu Ile Asn Thr Thr Gly Leu 225 230 235 240 Val Gln Being Ser Asn Wing Leu Tyr Pro Leu Read Glu Gly Gly Val Ser 245 250 255 Wing Thr Gln Thr Arg Asn Val Lys Wing Glu Glu Asn Asp Gln Asp Arg 260 265 270 Gly Arg Asp Gly Asp Gly Val Asn Asn Leu Be Arg Asn Phe Leu Gly 275 280 285 Asn Ile Asn Ile Asn Ile Asn Be Gly Arg Asn Glu Glu Tyr Thr Be Trp Gly 290 295 300 Gly Asn Be Ser Trp Thr Gly Phe Thr Be Asn Asn Be Thr Gly His 305 310 315 320 Leu Be Phe

<210> 221 <211> 1200 <212> DNA

<213> Arabidopsis thaliana <400> 221

atggtttttt cttcatttcc tacttatcct gatcattcat caaactggca acaacaacat 60 caaccaatca caaccaccgt tggattcacg ggaaataaca caccatcccc tcccaccgca acagcaacaa acgcctccgc aacggcggag tcgctgttcc cggtggacct ggcgggttaa gaaagagcaa ggctagccaa cataccatta cctgaaacag gactcaacta acaccaaatt ctgttacttc aacaactaca ttctgcaaag catgccgtcg ttactggaca cgtggcggtg ggtggcggtt gccgtagaaa caaaagaacc aaaaacagca accagtagcg gtaacagcaa gtcacaagac agcgccacga cgagccatgg ctaacaatca gatgggacca ccttcttcgt ctgtcttctt acaacgcagg gttaatcccc ggacatgatc atacttggac ttggatcatc tttgcctcct cttaagctta gacaacttca ccttacaata cggtgccgtt tcagctcctt agcagtggag gagcggcggc tcttttaaac ggttttgacc aaccaacttc ctttaggcgg tttagacccg tttgatcaac aatccaggtt acggattggt taccgggtcg ggtcagtatc aaccttatct cctcttcttc gtctgcttca tcagctatgg ttagcttcag tgaaaatgga agatagtaac aatcagctca ggagacgaac aacagctctg gaatattcat ggcgctgctg acaagttcgt ggagtgaagt ctctaataat ttcagttctt <210> 222 <211> 399 <212> PRT

<213> Arabidopsis thaliana <400> 222

Met Val Phe Be Ser Phe Pro Thr Tyr Pro Asp 15 10

Gln Gln Gln His Gln Pro Ile Thr Thr Thr

20 25

Asn Ile Asn Gln Gln Phe Leu Pro His His Pro

35 40

Gln Gln Thr Pro Pro Gln Read His His Asn Asn

tcaaccaaca agcttcacca tccgaccagg ccttgaagtg gtctcactca ctctaaggag gcggtggagg gcaacgacca catcgctct ataacagcaa tgcctccttt cttatcatat agtggagatt

gtttcttcct caacaacggt ttcgatggcg tccaagatgt acctcgccac cgttcccgtc tggcggtagc attackcaccac aagctcgttg taacaacaac agacttcaca aggcggtgga cccggcaaca ggagcagcaggata cggcatcata cgtcgctc

His Ser Gly Phe

Ser Asn Trp 15

Thr Gly Asn 30

Pro Gln Gln

50

55

Read pro 45

Gly Asn Gly Gly Val 60

Val Wing Gly Pro Gly Gly Gly Gly Leu Ile Arg Pro Gly Be Met Wing 65 70 75 80 Glu Arg Wing Arg Leu 85 Wing Asn Ile Pro Leu 90 Pro Glu Thr Wing Read 95 Lys Cys Lys Phe Cys Tyr Phe 110 Asn Asn Tyr Being Read Thr Gln Pro Arg His Phe Cys Lys Cys Wing Arg Arg Tyr

115

120

Trp Thr Arg Gly Gly Wing Leu 130 135 Arg Arg Asn Lys Arg Thr Lys 145 150 Thr Be Ser Gly Asn Ser Lys 165 Gln

125

Val Gly Gly Gly Cys 140

Gly Gly

155

170

180

185

Ser Ser Ser Ser Ser Ser Ser Ser Leu Ser Ser

195

200

Ile Pro Gly His Asp His Asn Ser Asn Asn Asn

210 215

Gly Ser Ser Leu Pro Pro Leu Lys Leu Met Pro

225 230 235

Asp Asn Phe Thr Read Gln Tyr Gly Wing Val Ser

245 250

Ile Gly Gly Gly Be Ser Gly Gly Wing Wing

260 265

Asp Gln Trp Arg Phe Pro Wing Thr Asn Gln Leu

275 280

Asp Pro Phe Asp Gln Gln His Gln Met Glu Gln

290 295

Gly Leu Val Thr Gly Ser Gly Gln Tyr Arg Pro

Thr wing

Met gly

Tyr Asn 205 Asn Ile 220

Pro leu

Pro Wing

Leu Leu

Pro Leu 285 Gln Asn 300

Lys asn

Gly Gly Ser 160

To be asn asp

175 Pro Pro Ser 190

Wing Gly Leu

Leu Gly Leu

Asp Phe Thr 240

Be tyr his

255 Asn Gly Phe 270

Gly Gly Read Pro Gly Tyr Ile Phe His

120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 305

Asn Leu Ile Ser Ser 325

Thr Wing Ser Gln Leu 340

Leu Asn Leu Ser Arg 355

Ile His Gly Wing Wing 370

Ser Glu Val Ser Asn 385

<210> 223 <211> 52 <212> DNA <213> Artificial Sequence <220>

<223> initiator: prm07315 <400> 223

ggggacaagt ttgtacaaaa aagcaggctt aaacaatggg tggatcgatg gc 52

<210> 224 <211> 50 <212> DNA

<213> Artificial sequence <220>

<223> initiator: prm07316 <400> 224

ggggaccact ttgtacaaga aagctgggtc gttaatgatc cgacaaaaca 50

<210> 225

<211> 2193

<212> DNA

<213> Oryza sativa

<400> 225

aatccgaaaa gtttctgcac cgttttcacc ccctaactaa caatataggg aacgtgtgct 60

aaatataaaa tgagacctta tatatgtagc gctgataact agaactatgc aagaaaaact 120 catccaccta ctttagtggc aatcgggcta aataaaaaag agtcgctaca ctagtttcgt 180 tttccttagt aattaagtgg gaaaatgaaa tcattattgc ttagaatata cgttcacatc 240 tctgtcatga agttaaatta ttcgaggtag ccataattgt catcaaactc ttcttgaata 300 aaaaaatctt tctagctgaa ctcaatgggt aaagagagag atttttttta aaaaaataga 360 atgaagatat tctgaacgta ttggcaaaga tttaaacata taattatata attttatagt 420 ttgtgcattc gtcatatcgc acatcattaa ggacatgtct tactccatcc caatttttat 480 ttagtaatta aagacaattg acttattttt attatttatc ttttttcgat tagatgcaag 540 gtacttacgc acacactttg tgctcatgtg catgtgtgag tgcacctcct caatacacgt 600 tcaactagca acacatctct aatatcactc gcctatttaa tacatttagg tagcaatatc 660 tgaattcaag cactccacca tcaccagacc acttttaata atatctaaaa tacaaaaaat 720 aattttacag aatagcatga aaagtatgaa acgaactatt taggtttttc acatacaaaa 780 aaaaaaagaa ttttgctcgt gcgcgagcgc caatctccca tattgggcac acaggcaaca 840 acagagtggc tgcccacaga acaacccaca aaaaacgatg atctaacgga ggacagcaag 900 tccgcaacaa ccttttaac the gcaggctttg cggccaggag agaggaggag aggcaaagaa 960 aaccaagcat cctcctcctc ccatctataa attcctcccc ccttttcccc tctctatata 1020 ggaggcatcc aagccaagaa gagggagagc accaaggaca cgcgactagc agaagccgag 1080 cgaccgcctt cttcgatcca tatcttccgg tcgagttctt ggtcgatctc ttccctcctc 1140 cacctcctcc tcacagggta tgtgcccttc ggttgttctt ggatttattg ttctaggttg 1200 tgtagtacgg gcgttgatgt taggaaaggg gatctgtatc tgtgatgatt cctgttcttg 1260 gatttgggat agaggggttc ttgatgttgc atgttatcgg ttcggtttga ttagtagtat 1320 ggttttcaat cgtctggaga gctctatgga aatgaaatgg tttagggtac ggaatcttgc 1380 gattttgtga gtaccttttg tttgaggtaa aatcagagca ccggtgattt tgcttggtgt 1440 aataaaagta cggttgtttg gtcctcgatt ctggtagtga tgcttctcga tttgacgaag 1500 ctatcctttg tttattccct attgaacaaa aataatccaa ctttgaagac ggtcccgttg 1560 atgagattga atgattgatt cttaagcctg tccaaaattt cgcagctggc ttgtttagat 1620 acagtagtcc ccatcacgaa attcatggaa acagttataa tcctcaggaa caggggattc 1680 cctgttcttc cgatttgctt tagtcccaga attttttttc ccaaatatct taaaaagtca 1740 ctttctggtt cagttcaatg aattg attgc tacaaataat gcttttatag cgttatccta 1800 gctgtagttc agttaatagg taatacccct atagtttagt caggagaaga acttatccga 1860

310 315 320

Ser Ser Ser Ala Ser Ser Ala Met Val Thr Ala

330 335

Wing Ser Val Lys Met Glu Asp Ser Asn Asn Gln

345 350

Gln Leu Phe Gly Asp Glu Gln Gln Leu Trp Asn

360 365

Wing Wing Be Thr Wing Wing Wing Thr Be Being Trp

375 380

Asn Phe Ser Ser Ser Ser Thr Ser Asn Ile 390,395 tttctgatct ccatttttaa ttatatgaaa tgaactgtag cataagcagt attcatttgg 1920 attatttttt ttattagctc tcaccccttc attattctga gctgaaagtc tggcatgaac 1980 tgtcctcaat tttgttttca aattcacatc gattatctat gcattatcct cttgtatcta 2040 cctgtagaag tttctttttg gttattcctt gactgcttga ttacagaaag aaatttatga 2100 agctgtaatc gggatagtta tactgcttgt tcttatgatt catttccttt gtgcagttct 2160 tggtgtagct tgccactttc accagcaaag ttc 2193

<210> 226 <211> 1344 <212> DNA

<213> Arabidopsis thaliana <220>

<221> misc_feature <222> (196) .. (196) <223> η is a, c, g, or t <220>

<221> misc_feature <222> (461) .. (461) <223> η is a, c, g, or t <400> 226

atgatgatgg agactagaga tccagctatt aagcttttcg gtatgaaaat cccttttccg 60 tcggtttttg aatcggcagt tacggtggag gatgacgaag aagatgactg gagcggcgga 120 gatgacaaat caccagagaa ggtaactcca gagttatcag ataagaacaa caacaactgt 180 aacgacaaca gttttnacaa ttcgaaaccc gaaaccttgg acaaagagga agcgacatca 240 actgatcaga tagagagtag tgacacgcct gaggataatc agcagacgac acctgatggt 300 aaaaccctaa agaaaccgac taagattcta ccgtgtccga gatgcaaaag catggagacc 360 aagttctgtt attacaacaa ctacaacata aaccagcctc gtcatttctg caaggcttgt 420 cagagatatt ggactgctgg agggactatg aggaatgttc ntgtgggggc aggacgtcgt 480 aagaacaaaa gcttatcttc tcattaccgt cacatcacta tttccgaggc tcttgaggct 540 gcgaggcttg acccgggctt acaggcaaac acaagggtct tgagttttgg tctcgaagct 600 cagcagcagc acgttgctgc tcccatgaca cctgtgatga agctacaaga agatcaaaag 660 gtctcaaacg gtgctaggaa caggtttcac gggttagcgg atcaacggct tgtagctcgg 720 gtagagaatg gagatgattg ctcaagcgga tcctctgtga ccacctctaa caatcactca 780 gtggatgaat caagagcaca aagcggcagt gttgttgaag cacaaatgaa caacaacaac 840 aataacatga atggttatgc ttgcatccca ggtgttccat ggccttacac gtggaatcca 900 gcgatgcctc caccaggttt ttacccgcct ccagggtatc caatgccgtt ttacccttac 960 tggaccatcc caatgctacc accgcatcaa tcctcatcgc ctataagcca aaagtgttca 1020 aatacaaact ctccgactct cggaaagcat ccgagagatg aaggatcatc gaaaaaggac 1080 aacgagacag agcgaaaaca gaaggccggg tgcgttctgg tcccgaaaac gttgagaata 1140 gatgatccta acgaagcagc aaagagctcg atatggacaa cattgggaat caagaacgag 1200 gcgatgtgca aagccggtgg tatgttcaaa gggtttgatc ataagacaaa gatgtataac 1260 aacgacaaag ctgagaactc ccctgttctt tctgctaacc ctgctgctct atcaagatca 1320 cacaatttcc atgaacagat ttag 1344

<210> 227 <211> 447 <212> PRT

<213> Arabidopsis thaliana <220>

<221> UNSAFE <222> (66) .. (66)

<223> Xaa can be any naturally occurring amino acid <220>

<221> UNSAFE <222> (154). . (154)

<223> Xaa can be any naturally occurring amino acid <400> 227

Met Met Met Met Glu Thr Asp Pro Wing Ile Lys Leu Phe Gly Met Lys 1 5 10 15

Ile Pro Phe Pro Be Val Phe Glu Be Wing Val Thr Val Glu Asp Asp

20 25 30

Glu Glu Asp Asp Trp Being Gly Gly Asp Asp Lys Being Pro Glu Lys Val

35 40 45

Thr Pro Glu Read Asp Lys Asn Asn Asn Asn Asn Cys Asn Asp Asn Ser 50 55 60 Phe Xaa Asn Be Lys Pro Glu Thr Read Asp Lys Glu Ala Thr Be 65 70 75 80 Thr Asp Gln Ile Glu Be Ser Asp Thr Pro Glu Asp Asn Gln Gln Thr 85 90 95 Thr Pro Asp Gly Lys Thr Read Lys Lys Pro Thr Lys Ile Leu Pro Cys 100 105 110 Pro Arg Cys Lys Be Met Glu Thr Lys Phe Cys Tyr Tyr Asn Tyr 115 120 125 Asn Ile Asn Gln Pro Arg His Phe Cys Lys Cys Wing Gln Arg Tyr Trp 130 135 140 Thr Gly Wing Gly Thr Met Arg Asn Val Xaa Val Gly Wing Gly Arg Arg 145 150 155 160 Lys Asn Lys Be Read To Be His Tyr Arg His Ile Thr Ile Be Glu 165 170 175 Wing Leu Glu Wing Wing Arg Wing Read Asp Pro Gly Leu Gln Wing Asn Thr Arg 180 185 190 Val Leu Be Phe Gly Leu Glu Wing Gln Gln His Val Wing Pro Wing 195 200 205 Met Thr Pro Val Met Lys Leu Gln Glu Asp Gln Lys Val Ser Asn Gly 210 215 220 Wing Arg Asn Arg Phe His Gly the Asp Gln Arg Leu Val Wing Arg 225 230 235 240 Val Glu Asn Gly Asp Asp Cys Ser Ser Gly Ser Ser Val Thr Thr 245 250 255 Asn Asn His Ser Val Asp Glu Ser Arg Wing Gln Ser Gly Val Val 260 265 270 Glu Wing Gln Met Asn Asn Asn Asn Asn Asn Asn Met Asn Gly Tyr Cys 275 280 285 Ile Pro Gly Val Pro Trp Pro Tyr Thr Trp Asn Pro Ala Met Pro Pro 290 295 300 Pro Gly Phe Tyr Pro Pro Phe Tyr Pro Tyr 305 310 315 320 Trp Thr Ile Pro Met Leu Pro Pro His Gln Be Ser Pro Ile Ser 325 330 335 Gln Lys Cys Ser Asn Thr Asn Ser Pro Thr Read Gly Lys His Pro Arg 340 345 350 Asp Glu Gly Ser Ser Lys Lys Asp Asn Glu Thr Glu Arg Lys Gln Lys 355 360 365 Wing Gly Cys Val Leu Val Pro Lys Thr Leu Arg Ile Asp Pro Asn 370 375 380 Glu Wing Ala Lys Being Ser Ile Trp Thr Thr Leu Gly Ile Lys Asn Glu 385 390 395 400 Met Cys Lys Wing Gly Gly Met Phe Lys Gly Ph Wing and Asp His Lys Thr 405 410 415 Lys Met Tyr Asn Asn Asp Lys Wing Glu Asn Be Pro Val Leu Be Wing 420 425 430 Asn Pro Wing Wing Leu Be Arg Be His Asn Phe His Glu Gln Ile 435 440 445 <210> 228 < 211> 63 <212> PRT

<213> Artificial sequence <220>

<223> DOF domain <220>

<221> UNSAFE <222> (50) .. (50)

<223> Xaa can be any naturally occurring amino acid <400> 228

Lys Pro Thr Lys Ile Leu Pro Cys Pro Arg Cys Lys Be Met Glu Thr 15 10 15

Lys Phe Cys Tyr Tyr Asn Asn Tyr Asn Ile Asn Gln Pro Arg His Phe 20 25 30 Cys Lys Cys Wing Gln Arg Tyr Trp Thr Wing Gly Gly Thr Met Arg Asn

35 40 45

Val Xaa Val Gly Ala Gly Arg Arg Lys Asn Lys To Be To Be To Be

50 55 60

<210> 229 <211> 11 <212> PRT

<213> Artificial sequence <220>

<223> reason 1 <400> 229

Lys Wing Leu Lys Lys Pro Asp Lys Ile Leu Pro 1 5 10

<210> 230 <211> 14 <212> PRT

<213> Artificial sequence <220>

<223> reason 2 <400> 230

Asp Asp Pro Gly Ile Lys Read Phe Gly Lys Thr Ile Pro Phe 1 5 10

<210> 231 <211> 11 <212> PRT

<213> Artificial sequence <220>

<223> reason 3 <400> 231

Ser Pro Thr Read Gly Lys His Ser Arg Asp Glu 1 5 10

<210> 232 <211> 16 <212> PRT

<213> Artificial sequence <220>

<223> reason 4 <400> 232

Read Gln Wing Asn Pro Wing Wing Read Be Arg Be Gln Asn Phe Gln Glu 1 5 10 15

<210> 233 <211> 32 <212> PRT

<213> Artificial sequence <220>

<223> reason 5 <400> 233

Lys Gly Glu Gly Cys Leu Trp Val Pro Lys Thr Leu Arg Ile Asp Asp 1 5 10 15

Pro Asp Glu Wing Wing Lys Be Ser Ile Trp Thr Thr Read Gly Ile Lys 20 25 30

<210> 234

<211> 1263

<212> DNA

<213> Oryza sativa

<400> 234

atgaacaacg ttgaagagaa agcagcatca gactcaaaag atgaaaatga aaagacagca 60

aatgatgaat caggccagga caaagtactt aagaagccag ataagattct cccttgccct 120 cggtgcaaca gtatggacac aaagttttgt tattacaaca actacaatgt taatcaaccc 180 aggcacttct gtaagaactg ccaaaggtat tggactgccg ggggaaccat gagaaatgta 240 cctgttggtg ctgggaggcg caaaagcaag agctcatcgt tgcactaccg tcacttactg 300 atggcccctg attgcatgat ggggtctaga gtggaaatat ccaagtcaat gaaccctgaa 360 gctttcgcat ctgcgcattc gacccctata caaccaattg gcagaaacga aacagttctc 420 aaatttgggc cagaatggaa atctcttcaa agcacgccgg tacatgtacc atgccagagt atgaactccc ctagtgccac ccgtggatca tcaggcaatg gacaaggagg gcggcgaaga aagccgttcc cgtgctctta tga

<210> 235 <211> 420 <212> PRT <213> Oryza <400> 235 Met Asn Asn 1

Glu Lys Thr

Pro Asp Lys 35

Phe Cys Tyr 50

Lys Asn Cys 65

For Val Gly

ctgaggtgcc ccaatgctgc tcacatcaca tgtattgcaa catggaacat ctgcttccca ccatgatgcc ctgcactatg gaacgaatgg gctcccctct agaaatcact gctccatctg agtccaaagg aggcaaaccc

actctgtgaa agcagtacca caacgtgtta cggagtcggc aggatggaac atctgagagc ggttgcctcg gggttgctta cggctgcatg ggggaagcat gtgggttccc ggccaccctg tgagagcac

tcgatggcat acgggtgaaa cctgaaaatg ccagtgccgc aacgttccta tgcagcacca aggctttctg tccagctggc tctccatcgt tccagggact aagacgctcc gggatcaagc ggccaagcgtcgccc

cagtgctgaa atcaggaaga cagcccaagt agtactacct tgatggtgcc gttcagctcc caccaccatt cggccacggc cgtcgagcaa cctctctccc gcatcgacga ctggagaccc catcagagac agtcgttcca

cattcaggag taactcttgc tgacaagaac tggagctcct aggtacaagc atggatgaac tccataccct atggaacata cagcagctgt actgaaggag tcccgacgag tggcatcttc tcgtcctgct ggagacttct

Arg

Ile

Pro

Glu 145 Gln

Asp

Asn

Val

Trp 225 Met

Pro

To be

Cys

Thr 305 Ser

Pro

His

To be

Ile 130 Val

Asn

Asn

Allah

Gly 210 Asn

Pro

Trp

Allah

Read 290 Asn

Gly

Read

Read

Lys 115 Gln

Pro

Gly

To be

Wing 195 Pro

Ile

Glu

Met

Pro 275 Ser

Gly

Asn

Lys

sativa

Val Glu 5

Asn Wing 20

Ile leu

Tyr asn

Gln arg

Gly wing

85

Read Met 100

Be met

To Ile

Read Cys

Thr Asn 165 Cys Ile 180

Gln Val

Val pro

Gly trp

Ser Ala 245 Asn Met 260

Pro phe

Be trp

Gly cys

Gly Ser 325 Glu Asp

Glu Lys Wing Asp Wing Glu Pro Cys

Asn tyr

55 Tyr Trp 70

Arg Arg

Pro Wing

Asn pro

Gly Arg 135 Glu Ser 150

Wing wing

To be to be

Asp Lys

Gln Tyr 215 Asn Asn 230

Be gln

Asn ser

Pro tyr

Pro Wing 295 Met Ser 310

Pro leu

Be gly

25 Pro Arg 40

Asn val

Thr wing

Lys Ser

Asp Cys 105 Glu Wing 120

Asn glu

Met wing

Val Wing

Ile Thr 185 Asn Ser 200

Tyr leu

Val pro

Be glu

Pro Met 265 Pro Leu 280

Thr wing

To Be Gly Lys Lys Glu

Ser Asp 10

Gln asp

Cys asn

Asn gln

Gly Gly 75

Lys Ser 90

Met met

Phe wing

Thr val

Ser Val 155 Pro Thr 170

To be his

Thr pro

Gly Wing

Met Met 235 Ser Cys 250

Met pro

Val pro

Trp asn

Ser Ser 315 His Ser 330

To be read

To be lys

Lys val

Be met

45 Pro Arg 60

Thr met

To be to be

Gly ser

Ser Ala 125 Leu Lys 140

Read asn

Gly Glu

Asn val

Val Tyr 205 Pro Tyr 220

Val pro

To be thr

Val Wing

Pro Wing 285 Ile Pro 300

Ser Asn Arg Asp Trp Val

Asp Glu Asn 15

Read Lys Lys 30

Asp Thr Lys

His Phe Cys

Arg Asn Val 80

Read His Tyr 95

Arg Val Glu 110

His ser thr

Phe Gly Pro

Ile Gln Glu 160

Asn Gln Glu

175 Leu Pro Glu 190

Cys Asn Gly

Met tyr pro

Gly Thr Ser 240

Be Be Wing

255 Ser Arg Leu 270

Read Trp Gly

Trp Ile Arg

Ser Ser Cys 320

Ser Ser Leu

335 Pro Lys Thr

480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1263 340 345 350 Leu Arg Ile 355 Asp Asp Pro Asp Glu 360 Wing Lys Ser Ser 365 Ile Trp Thr Wing Leu 370 Gly Ile Lys Pro Gly 375 Asp Pro Gly Ile Phe 380 Lys Pro Phe Gln Be Lys Gly Glu Be Lys Gly Gln Wing Wing Be Glu Thr Arg Pro Wing 385 390 395 400 Arg Wing Leu Lys Wing 405 Asn Pro Wing Wing Leu 410 Be Arg Be Gln Ser 415 Phe Gln Glu Thr Be 420

<210> 236

<211> 1365

<212> DNA

<213> Oryza sativa

<400> 236

atgcctaatt taggaaatgg tgtaaaaacc aataatgact tacctttagt ctcagacaag 60 cttttgattg tcaaaggtat tcctttttgt cccaacaata gtaagaaaaa tgatttacaa 120

ggcatcagca gaccggatgg aagaatagaa atcgattcca tgactgagga tgttaagact 180 gagcctgatg gatctgtccc tgagaagata ctcaagaagc cagataagat tctgccatgt 240

ccacgctgca atagcatgga aacaaagttc tgctacttca acaactacaa tgttcaccag 300

cccaggcact tctgcaggaa ctgccaaaga tattggaccg ctggtggagc tatgaggaat 360

gtcccagttg gtgctggaag acgcagaaat aagcatgtgt caaaatactg tcaggcgatg 420

atgacgtgca ataatactgt agctcctgga gatgtttctg atgtggttca ccatcaggtt 480

attacacatg gatcttctct ccttccagca acactgaagg aaaatgaaac acctacagaa 540

ttcatatcag aagtaccacc atgtaagtct tcagcttcaa tccttgatat tggagagccg 600

aatgatactg accttgttcc cttggcctct ggtgataaca aggaagaaaa atcatgtgca 660

tcttctgtgg tagtatccag ctgttcagag aatctgatgc cagataatgc aattatgaaa 720

gagccaaaca acaggtcagg atgttgtaat ggtgtggcat tgcctttccc tactggacct 780

gctttggtgc tcccctggag tcttggatgg aacagtgttg ctctcatgcc agctacccag 840

tgctcgatgc agcccgttct tgggttaaaa gatgggatac cctgcccgcc ttcatggcca 900

ccgcaactga tggtgccggc cccaggaatc tgtactcctg ttgttccaat ccctcttgtg 960

ccacctctgt ggagctgctt ccctggctgg cctaatggaa tgtggaatgc acaatgccct 1020

ggaggtaata ctactgtgct gccgtcaacc gctccaaaca aaatttcttg ttcaggaagc 1080

agttctctgg tgttgggaaa gcattcaaga gaagaaagct tgcaggaaga agagaagacg 1140

agaaattact tatgggtacc taaaactctt aggattgatg atccagctga ggctgcaaag 1200

agttcaatct gggcgaccct tggcatcaag cctgacgata aaggcatatt caagtctttc 1260

cagccaaatg ttgcgaaaaa tggcacggca ccagaatcgc ctcaggctct gcaggccaat 1320

ccagcagcat tttcgcgttc tcaatcgttt caagagacga cttga 1365 <210> 237 <211> 454 <212> PRT

<213> Oryza sativa <400> 237 Met 1

Pro Asn Leu Gly Asn Gly Val Lys Thr Asn Asn Asp 5 10

Val Ser Asp Lys Leu Leu Ile Val Lys Gly Ile Pro Phe

20

25

Leu Pro Leu 15

Cys Pro Asn 30

Asn Ser Lys 35 Lys Asn Asp Leu Gln 40 Gly Ile Ser Arg Pro 45 Asp Gly Arg Ile Glu 50 Ile Asp Ser Met Thr 55 Glu Asp Val Lys Thr 60 Glu Pro Asp Gly Ser 65 Val Pro Glu Lys Ile 70 Leu Lys Lys Pro Asp 75 Lys Ile Leu Pro Cys 80 Pro Arg Cys Asn Ser 85 Met Glu Thr Lys Phe 90 Cys Tyr Phe Asn Asn 95 Tyr Asn Val His Gln 100 Pro Arg His Phe Cys 105 Arg Asn Cys Gln Arg 110 Tyr Trp Thr Wing Gly 115 Gly Wing Met Arg Asn 120 Val Pro Val Gly Wing 125 Gly Arg Arg Arg Asn 130 Lys His Val Ser Lys 135 Tyr Cys Gln Met Wing 140 Met Thr Cys Asn As Val Thr Pro Wing Val Gly Asp Val Be Asp Val Val His His Val Gln 145 150 155

Ile Thr His Gly Ser Serve Leu Pro Wing Ward

165 170

Thr Pro Thr Glu Phe Ile Be Glu Val Pro

180 185

Ser Ile Read Asp Ile Gly Glu Pro Asn Asp Thr

195 200

Ala Ser Gly Asp Asn Lys Glu Glu Lys Ser Cys

210 215

Val Ser Ser Cys Ser Glu Asn Leu Met Pro Asp 225 230 235

Glu Pro Asn Asn Arg Be Gly Cys Cys Asn Gly

245 250

Pro Thr Gly Pro Wing Leu Val Leu Pro Trp Ser

260 265

Val Wing Read Met Pro Wing Pro Gln Cys Ser Met

275 280

Read Lys Asp Gly Ile Pro Cys Pro Pro Ser Trp

290

295

Val Pro Pro Wing Gly Ile Cys 305

Thr

Read Lys Glu

Cys Lys Ser 190

Asp Leu Val

205 Ser Ser Wing 220

Asn Ala Ile

Val Wing Leu

Read Gly Trp 270

Gln Pro Val

285 Pro Pro Gln 300

Pro Ile Pro

Pro val val

310 315 Pro Pro Read Trp Be Cys Phe Pro Gly Trp Pro Asn Gly Met

325 330

Gln Cys Pro Wing Gly Gly Asn Thr Thr Val Leu

340

345

Asn Lys Ile To Be Cys To Be Gly To Be To Be Leu

355

360

Be Arg Glu Glu Be Leu Gln Glu Glu Glu Lys

370

375

Trp Val Pro Lys Thr Leu Arg Ile Asp Asp Pro

Pro Ser Thr 350

Val Leu Gly

365 Thr Arg Asn 380

Wing Glu Wing

385

390

395

Be Ser Ile Trp Wing Thr Gly Ile Lys Pro Asp Asp Lys

405

410

Phe Lys Ser Phe Gln Pro Asn

420

425

Ser Pro Gln Wing Read Gln Wing Asn Pro Wing Wing

435 440

Be Phe Gln Glu Thr Thr

450 <210> 238 <211> 1461 <212> DNA <213> Oryza sativa <400> 238

atgggagggg gtggtaagtg caaaagaggg aaaagaggaa aggggccagt gttgcagcag cagcagcagc agcggaggcg agagaatcag aggagagctt gggtgagatg ggtgagtgca gatgggctga tcaagctgtt cgggaagacg atcccggtgc cagcagcata gtggcagtag cagcagctca accgaatccg gtcgccgtcg ccgacccctc cccgcggtcg gaggtcgtcg ccgggcggcg aggcggcgag ccatcagcag cagcagaagg gacaagatcc tgccatgccc gcggtgcagc agcatggaca aactacaacg tcaaccagcc tcgccacttc tgcaagcact ggcggcgcca tgcgcaacgt ccccgtcggc gccggccgcc gccgccgccc acttcctcca ccgcgtccgc gcctgcgccg gcgccccacg acgccaccaa cgccaccgtg ctcagcttcg gacgcgccgc cggtcaccct ggacctcgcc gacaagatga ctcgtcgccc atgcccggaa cgccgacgcc gccgccgcgt agggacgacg agcagatcgg caacactgta gcaaaacctg cctcctcctc ctcatcatca tcatcattca gccatgaacg tacacctcgg ggatcgcgat cccgatatac ccggcggcgc attccacctc ctggagcttg gagcctccca tggccggcca tcatcatcat caccacctac aagtgctaca ccttcagtct

Gly Thr Wing 430

Phe Ser Arg 445

160 Asn Glu 175

To be a wing

Pro leu

Val Val

Met Lys 240 Pro Phe 255

Asn ser

Read gly

Read met

Leu Val 320 Trp Asn 335

Pro Wing

Lys his

Tyr leu

Lys 400 Wing Gly Ile 415

Pro Glu Ser Gln

agattgcagc caagaagacg gaggaggagg agccggatgc acgtccaaga acggcgagag agatgaagct ccaagttctg gccagcgcta gcaagaacaa ccgccgccgc gcggcggcgg cgcgcctcgg gcagcgaggt caaacgggtt gtggcggcat cggcgtactg cagtccagtc cctccttcac

caagcgaaga agctagagag aggaggagga caaggatgtt aaccgccgct cccgccgcag gaagaagccg ctacttcaac ctggaccgcc gaacgccacc catgcccggacac caaggagggacg gtcgagcaac gcgggcaggagcaggagcaggaggaggag

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 catcccagag agggtggtga tcatgaggca agagatcacc atggcaatgg taaagtgtgg 1200

gtgccgaaga cgatccggat cgacaacgcc gacgaggttg cccggagctc aatccggtca 1260

ctcttcgcct tcagaggcgg cgacaaggcg gatgataaca acgacgacga tggcaccggc 1320

gtgcacaagc tcgccaccac ggtgttcgag ccaaagaggg acagcaagac ggcgaaacat 1380

ccggcgatca cgagcttgcc gctcttgcac accaaccccg tcgcgcttac ccgatccgcg 1440

accttccagg agggatcttg at 1461 <210> 239 <211> 486

<212> PRT <213> Oryza sativa <400> 239 Met Gly Gly Gly Gly Lys Cys Lys Arg Gly Lys Cys Lys Ily Wing 1 5 10 15 Wing Lys Arg Arg Gly Gln Cys Cys Ser Ser Ser Ser Ser Gly 20 25 30 Gly Wing Arg Arg Arg Wing Arg Arg Arg Glu Arg Glu Be Glu Glu Be Leu Gly 35 40 45 Glu Met Gly Gly Gly Gly Gly Gly Gly Gly Asly Gly Leu Ile 50 55 60 Lys Leu Phe Gly Lys Thr Ile Pro Val Gln Pro Asp Wing Lys Asp Val 65 70 75 80 Gln Gln His Be Gly Being Being Being Being Being Being Thr Glu Being Asp Val Gln 85 90 95 Glu Thr Ward Wing Val Val Wing Wing Asp Pro Being Pro Arg Being Glu Val 100 105 110 Val Asp Gly Glu Be Pro Pro Gln Gly Gly Glu Wing Ala Be His 115 120 125 Gln Gln Gln Gln Lys Glu Met Lys Leu Lys Pro Asp Lys Ile Leu 130 135 140 Pro Cys Pro Arg Cys Be Met Asp Thr Lys Phe Cys Tyr Phe Asn 145 150 155 160 Asn Tyr Asn Val Asn Gln Pro Arg His Phe Cys Lys His Cys Gln Arg 165 170 175 Tyr Trp Thr Wing Gly Gly Wing Met Arg Asn Val Pro Val Gly Wing Gly 180 185 190 Arg Arg Lys Asn Lys Asn Wing Thr Wing Wing His Phe Leu His Arg 195 200 205 Val Arg Wing Cys Ala Ala Ala Ala Met Ala Pro Ala Ala Pro Asp 210 215 220 Ala Thr Asn Ala Thr Val Leu Be Phe Gly Gly Gly Gly Gly His 225 230 235 240 Asp Ala Pro Pro Val Leu Asp Leu Asp Lys Met Thr Arg Leu 245 250 255 Gly Lys Glu Gly Leu Val Wing His Wing Arg Asn Wing Asp Wing Wing Wing 260 265 270 Cys Wing Be Glu Val Ser Be Asn Arg Asp Glu Gln Ile Gly Asn 275 280 285 Thr Val Wing Lys Pro Wing Asn Gly Leu Gln Gln His Pro Pro Pro 290 295 300 His His His His His Be Ala Met Asn Gly Gly Gly Ile Trp Pro Tyr 305 310 315 320 Tyr Thr Be Gly Ile Alle Ile Pro Ile Tyr Pro Ala Pro Ala Tyr 325 330 335 Trp Gly Cys Met Ile Pro Pro Gly Wing Trp Ser Leu Pr the Trp Pro 340 345 350 Wing Thr Val Gln Be Gln Wing Ile Be Ser Be Be Pro Pro Thr Be 355 360 365 Wing Thr Pro Be Val Be Phe Thr Read Gly Lys His Pro Arg Glu 370 375 380 Gly Gly Asp His Glu Wing Arg Asp His His Gly Asn Gly Lys Val Trp 385 390 395 400 Val Pro Lys Thr Ile Arg Ile Asp Asn Wing Asp Glu Val Wing Arg Be 405 410 415 Ser Ile Arg Ser 420 Leu Phe Wing Phe Arg 425 Gly Gly Asp Lys Wing 430 Asp Asp Asn Asn Asp 435 Asp Asp Gly Thr Gly 440 Val His Lys Leu Wing 445 Thr Thr Val Phe Glu 450 Pro Lys Arg Asp Ser 455 Lys Thr Wing Lys His 460 Pro Ala Ile Thr Be Read Leu Read His Thr Asn Pro Val Wing Read Thr Thr Ser Wing 465 470 475 480 Thr Phe Gln Glu Gly Ser

485

<210> 240

<211> 1461

<212> DNA

<213> Oryza sativa

<400> 240

atgggagggg gtggtaagtg caaaagaggg aaaagaggaa

aggggccagt gttgcagcag cagcagcagc agcggaggcg

agagaatcag aggagagctt gggtgagatg ggtgagtgca

gatgggctga tcaagctgtt cgggaagacg atcccggtgc

cagcagcata gtggcagtag cagcagctca accgaatccg

gtcgccgtcg ccgacccctc cccgcggtcg gaggtcgtcg

ccgggcggcg aggcggcgag ccatcagcag cagcagaagg

gacaagatcc tgccatgccc gcggtgcagc agcatggaca

aactacaacg tcaaccagcc tcgccacttc tgcaagcact

ggcggcgcca tgcgcaacgt ccccgtcggc gccggccgcc

gccgccgccc acttcctcca ccgcgtccgc gcctgcgccg

gcgccccacg acgccaccaa cgccaccgtg ctcagcttcg

gacgcgccgc cggtcaccct ggacctcgcc gacaagatga

ctcgtcgccc atgcccggaa cgccgacgcc gccgccgcgt

agggacgacg agcagatcgg caacactgta gcaaaacctg

cctcctcctc ctcatcatca tcatcattca gccatgaacg

tacacctcgg ggatcgcgat cccgatatac ccggcggcgc

attccacctc ctggagcttg gagcctccca tggccggcca

tcatcatcat caccacctac aagtgctaca ccttcagtct

catcccagag agggtggtga tcatgaggca agagatcacc

gtgccgaaga cgatccggat cgacaacgcc gacgaggttg

ctcttcgcct tcagaggcgg cgacaaggcg gatgataaca

gtgcacaagc tcgccaccac ggtgttcgag ccaaagaggg

ccggcgatca cgagcttgcc gctcttgcac accaaccccg

accttccagg agggatcttg at <210> 241 <211> 440 <212> PRT

<213> Oryza sativa <400> 241

Met Gly Glu Cys Lys Val Gly Gly Gly

15 10 Ile Lys Leu Phe Gly Lys Thr Ile Pro Val Pro

20 25

Wing Wing Gly Asp Val Asp Lys Asp Read Gln His

35 40

Thr Glu Pro Lys Thr Gln Glu Asn

50 55

Pro Pro Gln Pro Pro Glu Val Val Asp Thr Glu Asp

agattgcagc caagaagacg gaggaggagg agccggatgc acgtccaaga acggcgagag agatgaagct ccaagttctg gccagcgcta gcaagaacaa ccgccgccgc gcggcggcgg cgcgcctcgg gcagcgaggt caaacgggtt gtggcggcat cggcgtactg cagtccagtc cctccttcac atggcaatgg cccggagctc acgacgacga acagcaagac tcgcgcttac

caagcgaaga agctagagag aggaggagga caaggatgtt aaccgccgct cccgccgcag gaagaagccg ctacttcaac ctggaccgcc gaacgccacc catgcccgcg aggcggacac caaggagggg gtcgagcaac gcagcagcat ctggccctac gggctgcatg tcaggccatc actaggcaag taaagtgtgg aatccggtca tggcaccggc ggcgaaacat ccgatccgcg

Gly Gly

Glu Pro

Ser Gly 45

Asp Ser 60

To be to be

65

70

75

Asn Be Ser Glu Asn Gln Gln Gln Gln Gly Asp Thr Wing

85 90

Glu Lys Leu Lys Pro Asp Lys Ile Leu Pro Cys Pro

100

105

Being Met Asp Thr Lys Phe Cys Tyr Tyr Asn Asn

115 120

Pro Arg His Phe Cys Lys Asn Cys Gln Arg Tyr 130 135

Tyr Asn 125 Trp Thr 140

Asp Cys Leu 15

Gly Wing Cys 30

Be Ser Thr

Thr ser pro

Wing Asp Lys 80

Asn Gln Lys 95

Arg Cys Ser 110

Ile Asn Gln Wing Gly Gly

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1461 Met Arg Wing Asn Val Pro Val Gly 145 150

Val Ser Wing Al Ser Ser Phe Leu 165

Gly Asp Pro Pro Read Tyr Wing Pro 180

Ser Phe Gly Ser Asp Read Ser Thr 195 200

His Leu Lys Asp Lys Phe Ile Pro

Gly Arg Wing 155

Gln Arg Val

170 Val Lys Thr 185

Read Asp Read Thr Thr Gly

210

215

Glu Met Pro Val Gly Leu Cys Wing Glu Gly Leu

Arg

Arg

Asn

Thr

Ile 220 Ser

Lys

Allah

Gly

Glu 205 Lys

To be

Allah

Thr 190 Gln

Asn

Lys thr

225

230

235

Lys Ser 160 Leu Pro 175

Val leu

Met lys

Thr asp

Glu Glu 240

Ser Asn Gln Thr Asn 245 Leu Lys Glu Lys Val 250 Ser Asp Wing Asp Arg Ser 255 Pro Asn Val Wing Gln 260 His Pro Cys Met Asn 265 Gly Gly Wing Met Trp 270 Pro Phe Gly Val Wing 275 Pro Pro Wing Tyr 280 Tyr Thr Ser Ser Ile 285 Ile Pro Wing Phe Tyr 290 Pro Wing Wing Wing Wing 295 Wing Val Wing Wing Tyr 300 Trp Gly Cys Met Val Pro Gly Wing Trp Asn Pro Pro Wing Gln Ser Gln Ser Gln 305 310 315 320 Ser Val Ser Ser Ser 325 Ser Wing Ala Ser Pro 330 Val Ser Thr Thr Thr 335 Asn Cys Phe Arg Leu 340 Gly Lys His Pro 345 Asp Gly Asp Glu Glu 350 Leu Asp Ser Lys Gly 355 Asn Gly Lys Val Trp 360 Val Pro Lys Thr Val 365 Arg Ile Asp Asp Val Asp Glu Val Wing Arg Be Ser Ile Trp Ser Leu Ile Gly Ile

370

375

Lys 385

Gly Asp Lys Val Gly Wing Asp 390

Lys Val Phe Glu Ser Lys Asp Glu 405

Ile Ser Ser Leu Pro Phe Met Gln 420

Ser Val Thr Phe Gln Glu Gly Ser 435 440

<210> 242 <211> 1803 <212> DNA <213> Oryza sativa <400> 242

acttgtcgga tctctctctc tctctctctc catggacgac ctcgccgccg cctcccctcc cgtgcctccg cccccgcaga cgccggagaa gatcagtgaa gaaaagccat gcacagatca tagctttaat agttccagcg agtgtgagaa atcagagtcc aaatctgagg cagctcaaac cctgaagaag ccagacaaga tcctgccttg ctgctactac aacaactaca acattaacca atattggacg gcaggtggaa gcatgaggaa taagagctcc actgcaaatt accgcagtat tgctggagat gctcccctct atcaactctc taaatttgca cctgattccc cactctgtaa gcagagtaag aatgccaagc ctacctcaac ctgcccggct tcaggaacaa cttcagatag tagtgggcat caaaatggaa ttgttgggca atgcttccct ggtcctcctt ttgtgtaccc catggcacca ccggtatgca cagcaccagc cacagctagt gttcagtgga gcatgccacc aattccatct tcagtttggc ccttcatttc atggattcaa cctaattgca gcgtgtcagc

His Gly Arg 395

Wing Lys Wing

410 Gly Asn Pro 425

380 Gly

To be

Allah

Cys Thr Wing

Lys

His

Read 430

Leu Wing 400 Thr Wing 415

Thr arg

tccctctctc tcacccgccg ggattcatgt ggagttagat tcaaacacct agagggtggt tcctcgttgc gccaagacat cctccctgtt attaatcacg tataaaagga ttccatggcc agcacaaccc tggagcgtgtcgtgtgtcgctg

tctcgcccat ccgccgccgc gaggatacag gctgatcaga agcaatgatg ggatcgagtg aacagcatgg ttttgcaaga ggtgctggta ggcagcaatc gatcaaacag tctgtgctga agaaatggag gagcaggagcatgagcgtgagcgggccat

gtaccatctc cggaatccca gagacatgag tgaatagttc aaatgactgg aagagaaggt atacaaagtt gttgtcagag ggcgcaagag tagctgctcc caacggcagt aaattggaga aaacccagac atggagcagt atcccatacc ggggcccctggccccgggccccgtg

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 agacaacggc tctcctgtcc taggaaagca ctccagggac tccaaaccgc aaggagatga 1260 caaggcagag aaaaacttgt ggattccgaa aacgcttcgg atcgatgatc ctgacgaagc 1320 tgcaaagagc tcaatctgga caacccttgg cattgaacct ggtgaccgta gcatgttcag 1380 atcattccag tcgaaacctg aaagcaggga gcagatatcc ggtgctgcac gagtcctgca 1440 ggcgaatcca gcagctctat ctcgatctca atctttccag gagacaacgt gatgtatatt 1500 gaagaaatcg tgtgacaatt gtagaacatg ttactactat aatttaagca agtgctacag 1560 cctgaaggat gattggcgat gtaggcgctg ctgcaaacat ggaggcagcg tgatctgtac 1620 tattgaaacc tgaagagtac tattcttgac atttttctat aatttgcctg tggagcatgc 1680 accagtctac tgggttcatg atcaggctgt cagcatatgt tttgttgtac aataataata 1740 attgttaata gctttgccta aaggatactg atagtattct gctgatcctt cagctctgtg 1800 to 1803

<210> 243 <211> 476 <212> PRT

<213> Oryza sativa <400> 243

Met Asp Asp Read Wing Wing Wing Wing Be Pro Pro His Pro Pro Pro Pro 1 5 10 15

Pro Glu Be His Val Pro Pro Pro Pro Gln Thr Pro Pro Glu Lys Asp Ser

20 25 30

Cys Glu Asp Thr Gly Asp Met Arg Ile Be Glu Glys Lys Pro Cys Thr

35 40 45

Asp Gln Glu Read Asp Wing Asp Gln Met Asn Ser Be Ser Phe Asn Ser

50 55 60

Be Be Glu Cys Glu Asn Gln Thr Pro Be Asn Glu Met Asp Gn 65 70 75 80

Be Glu Be Lys Be Glu Ward Wing Gln Thr Glu Gly Gly Gly Be Ser

85 90 95

Glu Glu Lys Val Leu Lys Lys Pro Asp Lys Ile Leu Pro Cys Pro Arg

100 105 110

Cys Asn Be Met Asp Thr Lys Phe Cys Tyr Tyr Asn Asn Tyr Asn Ile

115 120 125

Asn Gln Pro Arg His Phe Cys Lys Be Cys Gln Arg Tyr Trp Thr Wing

130 135 140

Gly Gly Ser Met Arg Asn Leu Pro Val Gly Wing Gly Arg Arg Lys Ser 145 150 155 160

Lys Be Be Thr Wing Asn Tyr Arg Be Ile Read Ile Thr Gly Be Asn

165 170 175

Leu Wing Pro Wing Gly Asp Wing Pro Wing Leu Tyr Gln Leu Ser Ile Lys

180 185 190

Gly Asp Gln Thr Wing Thr Wing Val Lys Phe Wing Pro Asp Be Pro Leu

195 200 205

Cys Asn Be Met Wing Be Val Leu Lys Ile Gly Glu Gln Be Lys Asn

210 215 220

Wing Lys Pro Thr Be Thr Wing Gln Pro Arg Asn Gly Glu Thr Gln Thr 225 230 235 240

Cys Pro Wing Be Gly Thr Thr Be Asp Be Pro Arg Asn Glu Pro Val

245 250 255

Asn Gly Ala Val Ser Gly His Gln Asn Gly Ile Val Gly His Ser Gly

260 265 270

Val Pro Pro Met His Pro Ile Pro Cys Phe Pro Gly Pro Pro Phe Val

275 280 285

Tyr Pro Trp Be Pro Wing Trp Asn Gly Ile Pro Wing Met Wing Pro Pro

290 295 300

Val Cys Thr Wing Pro Wing Glu Pro Wing Asn Be Ser Asp Asn Gly Ser 305 310 315 320

Thr Wing Be Val Gln Trp Be Met Pro Pro Val Met Pro Val Gly

325 330 335

Tyr Phe Pro Val Ile Pro Ser Be Val Trp Pro Phe Ile Pro Val Ser

340 345 350

Pro Asn Gly Wing Trp Be Ser Pro Trp Ile Gln Pro Asn Cys Ser Val

355 360 365

Be Ala Ser Be Pro Thr Be Thr Be Thr Cys Be Asp Asn Gly Be 370 375 380 Pro Val Leu Gly Lys His Be Arg Asp Be Lys Pro Gln Gly Asp 385 390 395 400 Lys Wing Glu Lys Asn Leu Trp Ile Pro Lys Thr Leu Arg Ile Asp Asp 405 410 415 Pro Asp Glu Wing Ala Lys Be Ser Ile Trp Thr Thr Read Le Gly Ile Glu 420 425 430 Pro Gly Asp Arg Be Met Ser Gly Wing Arg Wing Val Leu Gln Wing Asn Pro Wing 450 455 460 Wing Read Le Ser Arg Be Gln Ser Phe Gln Glu Thr Thr 465 470 475 <210> 244 <211> 1817

<212> DNA

<213> Hordeum vulgare <400> 244

ctagacatga ttgccaagct cgaaaataac cctcacaaag ggaacaaaac tggtaccggg 60

ccccccctcg aggtcgacaa ggtcggtgtc catgccccct ccccctcctc ctttgtgttc 120

ttgtgtatga aatcccggga attttgattg gtttctaata gagcctgtga aaaaaaaaaa 180

ggccatttcg aggcctgctt tctttggctt tggagccgat catggcgacg agtcgtgttt 240

ggtctagtca tcaggtctta agcatggagt tccctgcctg ttttgggagg tactagtaag 300

cgctaagcag gcaggtttag cttccattag caagggaatt tcgcctttgc tggtctctaa 360

taataatagc agtttcaaca gttttagtat tgcatgatct agtcgtctga acatctggac 420

ttctgaaaga caggcctttc taggttatta tcttgcctct tttaataggt cgttttcgcg 480

tgctcccaag gattttgctg aaagtggcta ctgatatgtg ttgccgtaac ctactggact 540

tcagaagttc agatgcgtcc gactctgagt acatcaccca ctgcaaatca aagcaccata 600

gcttcttttt ttcattaaca agatataaga actgggaata tgaacaaact ttgtcaaatt 660

ttacacttgg gtcagatggg ggaaagcatg ttgtaatcac cggtcaataa cccgaatatt 720

acaccgtgtt tgtggacctc taattcacaa caaaattatt tcaaagttag actatatata 780

tatacaagga actcatcgag attttctgaa tgtttacagg accttcagca cagaggaagc 840

accacggctg aaccgaaagt acaagaaagc gccccacagg actccacggg ctcgcctccg 900

cagccggagg ttgtggacgt cgaggatcca tctgctgtca agaactcagc agcagatcaa 960

caagaagaag aagaagaaca gggtgacacg gccaaccaga aggagaagct caagaagcct 1020

gacaagatcc tgccctgccc acgctgtagc agcatggaca ccaagttctg ctactacaac 1080

aactacaaca tcaaccagcc gcgccacttc tgcaagaact gccagaggta ctggacggcc 1140

ggcggggcca tgcgcaacgt gcccgtgggc gcgggccgcc gcaagagcaa gagcatatcg 1200

gcagcgtccc acttccttca gaggataagg gccgctctgc ccggtgatcc tctctgcacc 1260

ccggtcaaca ctaacggcac ggtgctcagc ttcggctccg acgcatccac cttggacgtc 1320

gtctcagaac agatgaagca catgaaggag ctcagctcag taacccggac cgagaacacc 1380

gatgccccgt cagtagggtc ttgtgctgaa ggatgggcaa agggagaaga gtcgagccag 1440

atgaactcga gggagagagt tgcagcagat agatcgccaa attttgcgca gcacccgtgc 1500

atgaacgggg cagccatgtg gccattcagt tgtgcaccat cgcctgccta tttcacccca 1560

aacgtagcaa ttccattcta tccagctgct gctgctgcct actggggctg catggttccg 1620

ggagcctgga acactccatg gcagccgcag ccgcagccgc agtctcagtg tcaatctagc 1680

tcaccaccta gtgccgcttc tccggtatca acaatgtcca gttgcttcca atcccggaag 1740

catcctagag atggtgatga ggaaagagat accaagggta atggcaaggt gtgggtgccc 1800

aagacgatcg agtcgac 1817 <210> 245 <211> 270 <212> PRT

<213> Hordeiam vulgare <400> 245

Leu 1 Lys Lys Pro Asp 5 Lys Ile Leu Pro Cys 10 Pro Arg Cys Ser Be 15 Met Asp Thr Lys Phe 20 Cyr Tyr Asn 25 Tyr Asn Ile Asn Gln 30 Pro Arg His Phe Cys 35 Lys Asn Cys Gln Arg 40 Tyr Trp Thr Wing Gly 45 Gly Wing Met Arg Asn 50 Val Pro Val Gly Wing 55 Gly Arg Arg Lys Be 60 Lys Be Ile Be Wing Wing Be His Phe Leu Gln Arg Ile Arg Wing Wing Read Gly Asp 65 70 75

Pro Read Cys Thr Pro Val Asn Thr Asn Gly Thr

85 90

Be Asp Wing Be Thr Read Asp Val Val Be Glu

100 105

Lys Glu Read Be Ser Val Thr Arg Thr Glu Asn

115 120

Val Gly Ser Cys Wing Glu Gly Trp Wing Lys Gly

130 135

Met Asn Be Arg Glu Arg Val Wing Wing Asp Arg 145 150 155

Gln His Pro Met Cys Asn Gly Wing Met Trp Wing

165 170

Pro Ser Pro Wing Tyr Phe Thr Pro Asn Val Wing

180 185

Wing Wing Wing Wing Wing Tyr Trp Gly Cys Met Val

195 200

Thr Pro Trp Gln Pro Gln Pro Gln Pro Gln Ser

210 215

Ser Pro Pro Ser Wing Al Ser Ser Pro Val Ser Thr 225 230 235

Gln Being Arg Lys His Pro Arg Asp Gly Asp Glu

245 250

Gly Asn Gly Lys Val Trp Val Pro Lys Thr Ile 260 265

<210> 246 <211> 1485 <212> DNA

<213> Cucurbita maxima <400> 246

cagctgcaaa agaggaaact cttcatgatt cagaggatta atgaggcgca catgaatcct gaagtgttat caacagatga ggaaaactga gaaagaacag aatgatgccc ccaactcgaa ataagatact tccatgtccc cgctgcaata gcatggaaac attataatgt caatcaacca cgccattttt gcaaagcctg gcggtaccat caggaatgtc cctgttggag ctggccgccg cacactaccg acacattaca atctcagagg ctctccgagc ttgaggtcaa ccacctagca tcaaaaggca atggacgagt cacctgtatg tgaatctatg gtcaatgtat cacatcctgc gaacaaggaa tgaatttgag ggagccaaag gaccttgtga attgttctag tgcatcttca gtaacaatgt caagctcaat tccctcaaga accacatatg cagaacatca atggttttcc ctggtgttcc ttggccttgt tcatggactg caccaatacc ctggagttcc tttatcattc taccctgcaa catattggag ggaatattcc ttgggtcact ccacaaccct gtcctccaat cgctaggcaa gcattcgaga gatggcgatg aactccaggc atcctccaaa acagaaaaat ggatctgttt tggttcccaa caaacgaagc tgctaagagt tcaatatggg agacacttgg aagctgttga tctgtctaat gttttccaat caaagggcga aagtgttgtc tccagttttg caagccaacc ctgcagcctt acgagcggtc gtgaatggta tttttgattt tccaggttca ttaagaagct taagcatgaa gtggatacaa gcatcaatct agcaatccag tttttcaaga atcccggaat tgttctctct tatcaagcca tccttttgta cataacttta catatgtgaa ctaaagattc tcagatgtat aaaaatgcag ccaaaagttt <210> 247 <211> 380 <212> PRT

<213> Cucurbita maxima <400> 247

Met Asn Pro Glu Val Leu Being Thr Asp Glu Asn 15 10

Arg Lys Thr Glu Lys Glu Gln Asn Asp Wing Pro

Val leu

Gln met

Thr Asp 125 Glu Glu 140

To be pro

Pro phe

Ile pro

Pro Gly 205 Gln Cys 220

Met Ser Glu Arg Glu Ser

To be Phe

95 Lys His 110

Pro Wing

To be to be

Asn phe

Ser Cys 175 Phe Tyr 190

Trp wing

Gln Ser

Be cys

Asp Thr 255

Thr 270

80 Gly

Met

To be

Gln

Wing 160 Wing

Pro

Asn

To be

Phe 240 Lys

tgcatgtgta aaatgataag agagaaactg caagttttgt tcaaagatat aaaaaacaag tgcacaaatt cctcaatttc agaaagaaag gggtggggaa gaagaatgga ttctcaaatc tccaccagcc ttgcagtgct ccctggtcca tgataattct aactttaagg tattaagaat cctaaagagt gtcaagatct ttgtaaaaga caagcttctt atagaaagag taaccgatgt gcttc

aaaacagcaa ctcgcaacaa aagaaaccag tattataata tggactgaag aactcagcct gatgttccta agtgtaagcc gtcttgaatg actggtgatg gccaggaggt ccatgtcttc ttgtgccctc tcaggttctt aattctccga gagatgaaag attgatgatc gattcaatca aacgtttctg cttactttcc aatctttaag ctgctcaaga tagctgccat agagtggcag

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1380 1440 1485

Asp Lys Leu Wing Thr 15

Asn Ser Lys Glu Lys 20 25 30

Leu Lys Lys Pro Asp Lys Ile Leu Pro Cys Pro Arg Cys Asn Be Met

35 40 45

Glu Thr Lys Phe Cys Tyr Tyr Asn Asn Tyr Asn Val Asn Gln Pro Arg

50 55 60

His Phe Cys Lys Cys Wing Gln Arg Tyr Trp Thr Glu Gly Gly Thr Ile 65 70 75 80

Arg Asn Val Pro Val Gly Ala Gly Arg Arg Lys Asn Lys Asn Ser Ala

85 90 95

Be His Tyr Arg His Ile Thr Ile Be Glu Wing Read Le Arg Wing Wing Gln

100 105 110

Ile Asp Val Pro Ile Glu Val Asn His Leu Wing Ser Lys Gly Asn Gly

115 120 125

Arg Val Leu Asn Phe Ser Val Ser Pro Pro Val Cys Glu Ser Met Val

130 135 140

Asn Val Be His Pro Wing Glu Arg Lys Val Leu Asn Gly Thr Arg Asn 145 150 155 160

Glu Phe Glu Gly Wing Lys Gly Pro Cys Glu Gly Gly Gly Glu Thr Gly Asp

165 170 175

Asp Cys Be Be Wing Be Be Val Thr Met Be Be Met Lys Asn

180 185 190

Gly Wing Arg Arg Phe Pro Gln Glu Pro His Met Gln Asn Ile Asn Gly

195 200 205

Phe Pro Ser Gln Ile Pro Cys Leu Pro Gly Val Pro Trp Pro Cys Ser

210 215 220

Trp Thr Wing Pro Ile Pro Pro Pro Leu Cys Pro Pro Gly Val Pro 225 230 235 240

Read Be Phe Tyr Pro Wing Thr Tyr Trp Be Cys Be Wing Be Gly Ser

245 250 255

Trp Asn Ile Pro Trp Val Thr Pro Gln Pro Cys Pro Ile Pro Gly

260 265 270

Pro Asn Be Pro Thr Read Gly Lys His Be Arg Asp Gly Asp Glu Leu

275 280 285

Gln Wing Asp Asn Be Glu Met Lys Asp Pro Pro Lys Gln Lys Asn Gly

290 295 300

Ser Val Leu Val Pro Lys Thr Leu Arg Ile Asp Asp Pro Asn Glu Wing 305 310 315 320

Wing Lys Being Ser Ile Trp Glu Thr Read Gly Ile Lys Asn Asp Being Ile

325 330 335

Lys Wing Val Asp Leu Be Asn Val Phe Gln Be Lys Gly Asp Leu Lys

340 345 350

Ser Asn Val Ser Glu Val Leu Ser Pro Val Leu Gln Wing Asn Pro Wing

355 360 365

Wing Read Be Arg Be Read Thr Phe His Glu Arg Be 370 375 380

<210> 248 <211> 1191 <212> DNA

<213> Arabidopsis thaliana <400> 248

atgtggctct ctcatctctt catgtccctc tctaagctga cgtgtaattt ctccattttc 60

tctgtcttta tggcttgtgg ctctattggt atgtctcaag ttagagatac tccggttaaa 120 ttgtttggct ggacaattac accggtttct catgatccat actcttcttc gtcccatgtt 180 cttcctgatt cttcctcgtc ttcctcgtct tcttctctat cacttcgacc acacatgatg 240 aataaccaat ctgttactga caatacaagt cttaagctgt catctaatct taacaacgag 300 tcaaaagaaa catctgagaa cagtgatgac caacacagcg agatcacaac aattacatcg 360 aaacaactga actgaagaaa ccagacaaga gaagaagaga ttcttccatg tccgagatgc 420 aacagcgcag acaccaaatt ctgttactac aacaactaca acgttaacca gccacgtcac 480 ttctgtagaa aatgccagag gtattggacc gctggtggat ccatgaggat cgtcccggtt 540 ggctcaggcc gtcgcaagaa caagggatgg gtttcttcag accagtacct gcacatcact 600 tccgaggata ctgacaatta caatagctcc tcaacaaaga ttctaagctt cgagtcttcg 660 gactctttgg taactgagag gcctaagcat caatcaaacg aagtgaagat aaacgctgaa 720 cctgtttcac aagaacccaa caacttccaa gggttacttc ctccccaagc atcccctgtt 780 tcgcctcctt ggccttacca ataccctcca aaccctagtt tctaccacat gcccgtctac 840 tggggctgcg cgataccggt ttggtctacc ctcgacactt ctacatgtct tgggaaaagg 900 acaagagacg aaact tctca tgaaactgtt aaagagagta aaaatgcttt tgagagaaca 960 agcttgcttt tggaatctca gagcatcaaa aatgaaacaa gtatggctac aaataaccat 1020 gtgtggtatc cagtaccgat gacccgcgag aagacacaag aattcagctt tttcagtaat 1080 ggagctgaaa caaagagcag caacaacaga ttcgtccctg aaacgtatct taacctgcaa 1140 gcaaaccctg cagccatggc aagatctatg aacttcagag the agagcatata 1191

<210> 249 <211> 396 <212> PRT

<213> Arabidopsis thaliana <400> 249

Met Trp Read Be His Read Phe Met Be Read His Be Read Lys Thr Cys Asn 1 5 10 15

Phe Ser Ile Phe Ser Val Val Phe Met Cly Gly Ser Ile Gly Met Ser

20 25 30

Gln Val Arg Asp Thr Pro Val Lys Read Phe Gly Trp Thr Ile Thr Pro

35 40 45

Val Be His Asp Pro Tyr Be Be Ser Be His Val Leu Pro Asp Be

50 55 60

To Be To Be To Be To Be To Be To Be To Be To Be To Be To Be For Arg To His Met 65 70 75 80

Asn Asn Gln Be Val Thr Asp Asn Thr Be Read Lys Read Be Ser Asn

85 90 95

Leu Asn Asn Glu Be As Lys Glu Thr Be Glu Asn Be Asp Asp Gln His

100 105 110

Be Glu Ile Thr Thr Ile Thr Be Glu Glu Glu Lys Thr Thr Glu Leu

115 120 125

Lys Lys Pro Asp Lys Ile Leu Pro Cys Pro Arg Cys Asn Ser Asp Wing

130 135 140

Thr Lys Phe Cys Tyr Tyr Asn Asn Tyr Asn Val Asn Gln Pro Arg His 145 150 155 160

Phe Cys Arg Lys Cys Gln Arg Tyr Trp Thr Wing Gly Gly Be Met Arg

165 170 175

Ile Val Pro Val Gly Ser Gly Arg Arg Lys Asn Lys Gly Trp Val Ser

180 185 190

Be Asp Gln Tyr Read His Ile Thr Be Glu Asp Thr Asp Asn Tyr Asn

195 200 205

Be Ser Be Thr Lys Ile Leu Be Phe Glu Ser Be Asp Ser Leu Val

210 215 220

Thr Glu Arg Pro Lys His Gln Ser Asn Glu Val Lys Ile Asn Glu Wing 225 230 235 240

Pro Val Ser Gln Glu Pro Asn Ashe Phe Gln Gly Leu Leu Pro Pro Gln

245 250 255

Wing Pro Pro Val Pro Pro Trp Pro Tyr Gln Tyr Pro Pro Asn Pro

260 265 270

Be Phe Tyr His Met Pro Val Tyr Trp Gly Cys Wing Ile Pro Val Trp

275 280 285

Be Thr Read Asp Thr Be Thr Cys Read Gly Lys Arg Thr Asp Glu

290 295 300

Thr Be His Glu Thr Val Lys Glu Be Lys Asn Wing Phe Glu Arg Thr 305 310 315 320

Ser Leu Leu Leu Glu Ser Gln Ser Ile Lys Asn Glu Thr Be Met Wing

325 330 335

Thr Asn Asn His Val Trp Tyr Pro Val Met Thr Thr Arg Glu Lys Thr

340 345 350

Gln Glu Phe Be As Phe Phe Be Asn Gly Wing Glu Thr Lys Be Asn

355 360 365

Asn Arg Phe Val Pro Glu Thr Tyr Leu Asn Leu Gln Wing Asn Pro Wing

370 375 380

Wing Met Wing Arg Be Met Asn Phe Arg Glu Ser Ile 385 390 395

<210> 250 <211> 1200 <212> DNA

<213> Arabidopsis thaliana <400> 250

atgtctaaat ctagagatac ggagataaag ttgtttggga ggacaatcac gatgtgaatt gttatgatcc gtcgtcgttg tcccctgttc acgatgtttc agcaaggagg attcgtcttc ttcttcatct tcttgttctc caactattgg gttccggtta aaaaaagtga gcaagagagt aacaaattca aagatccata gatctaaacg aaccaccaaa agcagtatct gagatttcat caccaagaag aactgtgatc aacagagcga gatcacaaca acaactacca caagtactac aaatcaacgg ctctcaagaa accggacaag cttattccat gtcctagatg aacaccaaat tctgttatta caacaactac aacgtgaacc agccacgtta aactgtcaga ggtattggac agctggtgga tctatgagga acgttcctgt cgtcgcaaga acaaaggatg gccttcttca aaccattact tgcaagtcac tgtgataata ataactcggg gacgatcctt agtttcggtt cttcggagtc gagactggta agcatcagtc aggtgataca gcaaagataa gtgctgattc gaaaataaaa gctaccaagg gtttcttcct ccgcaagtaa tgttacctaa ccttggcctt accaatggag tccaacgggt cctaacgcta gtttctaccc tactggggat gcacggttcc gatataccct acctcagaga cttcatcatg cggtcaagag atcaaactga aggaagaatc aatgatacta atacaacaat agagcaagat tggtctcaga atctcttaga atgaatatcg tggtctaagt taccgacaaa acccgagaaa aaaacgcaag tttgacacaa agggaaacag caacagaagt agcttggtct caagcaaacc ctgcagcgat gtctagagct atgaacttca <210> 251 <211> 399 <212> PRT

<213> Arabidopsis thaliana <400> 251

Met Be Lys Be Arg Asp Thr Glu Ile Lys Read Phe Gly 15 10

Thr Be Leu Read Asp Val Asn Cys Tyr Asp Pro Be Ser

20 25

Val His Asp Val Ser Be Asp Pro Be Lys Glu Asp Ser

35 40 45

Be Ser Be Cys Be Pro Thr Ile Gly Pro Ile Arg Val

50 55 60

Lys Be Glu Gln Glu Be Asn Lys Phe Lys Asp Pro Tyr 65 70 75

Asp Leu Asn Glu Pro Pro Lys Wing Val Ser Glu Ile Ser

85 90

Be Ser Lys Asn Asn Cys Asp Gln Gln Be Glu Ile Thr

100 105

Thr Thr Be Thr Thr Be Gly Glu Lys Be Thr Wing Leu 115 120 125

Asp Lys Leu Ile Pro Cys Pro Arg Cys Glu Ser Asn Wing

130 135 140

Cys Tyr Tyr Asn Asn Tyr Asn Val Asn Gln Pro Arg Tyr 145 150 155

Asn Cys Gln Arg Tyr Trp Thr Wing Gly Gly Ser Met Arg

165 170

Val Gly Ser Gly Arg Arg Lys Asn Lys Gly Trp Pro Ser

180 185

Tyr Leu Gln Val Thr Be Glu Asp Cys Asp Asn Asn Asn 195 200 205

Ile Read Be Phe Gly Be Be Glu Be Be Val Thr Glu

210 215 220

His Gln Ser Gly Asp Thr Wing Lys Ile Ser Wing Asp Ser 225 230 235

Glu Asn Lys Ser Tyr Gln Gly Phe Leu Pro Pro Gln Val

245 250

Asn Asn Ser Ser Pro Trp Pro Tyr Gln Ser Ser Pro Thr 260 265

atctctttta 60

ttctgatcca 120

accaatcagg 180

tatattatcc 240

ttccaagaac 300

atcaggagag 360

tgaaagcgca 420

cttctgcagg 480

tggctcaggt 540

ttctgaggat 600

ttcggttaca 660

agtttctcaa 720

taattcttct 780

tgtccccttc 840

tttaggaaaa 900

aactactaca 960

aagctagtaa gagcgctgtg 1020

gattcagttt gttcaatgga 1080

ccgaaacttc tcacagtcta 1140

gggagagcat gcaacaataa 1200

Arg Thr Ile 15

Read Ser Pro 30

To be to be

Pro Val Lys

Ile Leu Ser 80

Ser Pro Arg 95

Thr Thr Thr 110

Lys Lys Pro

Thr lys phe

Phe Cys Arg 160

Asn val pro

175 Ser Asn His 190

Be gly thr

Thr Gly Lys

Val Ser Gln 240

Met Leu Pro

255 Gly Pro Asn 270 Wing Be Phe Tyr Pro Val Pro Phe Tyr Trp Gly Cys Thr Val Ile 275 280 285 Tyr Pro Thr Be Glu Thr Be Cys Leu Gly Lys Arg Be Asp 290 295 300 Gln Thr Glu Gly Arg Ile Asn Asp Thr Asn Thr Thr Ile Thr Thr 305 310 315 320 Arg Wing Arg Leu Val Be Glu Be Read Arg Met Asn Ile Glu Wing Be 325 330 335 Lys Be Wing Val Trp Be Lys Leu Pro Thr Lys Pro Lys Lys Thr 340 345 350 Gln Gly Phe Be Leu Phe Asn Gly Phe Asp Thr Lys Gly Asn Be Asn 355 360 365 Arg Be Be Read Le Val Be Glu Thr Be His Be Read Gln Wing Asn Pro 370 375 380 Wing Ala Met Be Ala Met Asn Phe Arg Glu Being Met Gln Gln

390

395

385 <210> 252 <211> 1347 <212> DNA

<213> Arabidopsis thaliana <400> 252

atgatgatgg agactagaga tccagctatt aagcttttcg

tcggtttttg aatcggcagt tacggtggag gatgacgaag

gatgacaaat caccagagaa ggtaactcca gagttatcag

aacgacaaca gttttaacaa ttcgaaaccc gaaaccttgg actgatcaga tagagagtag tgacacgcct gaggataatc

aaaaccctaa agaaaccgac taagattcta ccgtgtccga

aagttctgtt attacaacaa ctacaacata aaccagcctc cagagatatt ggactgctgg agggactatg aggaatgttc

aagaacaaaa gctcatcttc tcattaccgt cacatcacta

gcgaggcttg acccgggctt acaggcaaac acaagggtct

cagcagcagc acgttgctgc tcccatgaca cctgtgatga

gtctcaaacg gtgctaggaa caggtttcac gggttagcgg

gtagagaatg gagatgattg ctcaagcgga tcctctgtga

gtggatgaat caagagcaca aagcggcagt gttgttgaag

aacaataaca tgaatggtta tgcttgcatc ccaggtgttc

ccagcgatgc ctccaccagg tttttacccg cctccagggt

tactggacca tcccaatgct accaccgcat caatcctcat

tcaaatacaa actctccgac tctcggaaag catccgagag

gacaacgaga cagagcgaaa acagaaggcc gggtgcgttc

atagatgatc ctaacgaagc agcaaagagc tcgatatgga

gaggcgatgt gcaaagccgg tggtatgttc aaagggtttg

aacaacgaca aagctgagaa ctcccctgtt ctttctgcta

tcacacaatt tccatgaaca gatttag <210> 253 <211> 448 <212> PRT

<213> Arabidopsis thaliana <400> 253

Met Met Met Met Glu Thr Arg Asp Pro Ile Lys Wing

gtatgaaaat aagatgactg ataagaacaa acaaagagga agcagacgac gatgcaaaag gtcatttctg ctgtgggggc tttccgaggc tgagttttgg agctacaaga atcaacggct ccacccgggggccgggggggtggggtg

cccttttccg gagcggcgga caacaactgt agcgacatca acctgatggt catggagacc caaggcttgt aggacgtcgt tcttgaggct tctcgaagct agatcaaaag tgtagctcgg caatcacgagagatggagtga

1

Ile

5 10

Pro Phe Pro Ser Val Phe Glu Ser Val Wing

20

25

Glu Glu Asp Asp Trp Being Gly Gly Asp Asp Lys

35

10

Thr Pro Glu Read Being Asp Lys Asn Asn Asn Asn

50

55

60

Phe Asn Asn Ser Lys Pro Glu Thr Read Asp Lys

65

70

75

Thr Asp Gln Ile Glu Be Ser Asp Thr Pro Glu

85

90

Thr Pro Asp Gly Lys Thr Read Lys Lys Pro Thr

100

105

Phe Gly Met Lys 15 Val Glu Asp Asp 30 Pro Glu Lys Val 45 Asn Asp Asn Be Glu Wing Thr Be 80 Asn Gln Thr 95 Ile Leu Pro Cys 110

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1200 1260 1320 1347 Pro Arg Cys Lys Be Met Glu Thr Lys

115 120

Asn Ile Asn Pro Gln Arg His Phe Cys

130 135

Thr Wing Gly Gly Thr Met Arg Asn Val 145 150

Lys Asn Lys Being Being Being Being His Tyr 165

Wing Read Glu Wing Wing Arg Wing Read Asp Pro 180 ~ 185

Val Leu Ser Phe Gly Leu Glu Wing Gln

195 200

Met Thr Pro Val Met Lys Leu Gln Glu

Allah

To be

Phe Cys Tyr Tyr Asn Asn Tyr 125

210

215

Arg Wing Asn Arg Phe His Gly Leu 225 230

Val Glu Asn Gly Asp Asp Cys Ser 245

Asn Asn His Ser Val Asp Glu Ser Arg 260 265

Glu Ala Gln Met Asn Asn Asn Asn Asn

275 280

Cys Ile Pro Gly Val Pro Trp Pro Tyr

290 295

Pro Pro Gly Phe Tyr Pro Pro Pro Gly 305 310

Tyr Trp Thr Ile Pro Met Leu Pro Pro 325

Ser Gln Lys Cys Ser Asn Thr Asn Ser 340 345

Arg Asp Glu Gly Being Ser Lys Lys Asp

355 360

Lys Wing Gly Cys Val Leu Val Pro Lys

370 375

Asn Glu Wing Wing Lys Ser Ser Ile Trp 385 390

Glu Wing Met Cys Lys Wing Gly Gly Met 405

Thr Lys Met Tyr Asn Asn Asp Lys Wing 420 425

Wing Asn Pro Wing Wing Read Be Arg Be

435 440

<210> 254 <211> 1374 <212> DNA

<213> Arabidopsis thaliana <400> 254

atggctgatc cggcgattaa gctctttgga aagacgattc gttgattctt cttctagcta taccggattt ttaaccgaaa tcagattcgt gtaccggcga tgatgatgat gaagagatgg gaagaaggtg atgatgttgg tgatggtgga ggagagagcg aaagatagtg agtgtcagga agagtcattg aggaatgaat acatcgggta taactgaaaa aacggaaaca acaaaagctg ggtggtactg cttgctctca agaggggaag ttaaagaaac ccgcgatgta acagcatgga aaccaagttc tgttactaca cctcgccatt tctgcaagaa atgtcagaga tattggacag gttccggttg gtgctgggag acgtaagaat aagagtccag gtaagtataa catctgcgga agctatgcag aaggtggcga aatggtgcaa atcttctcac ttttggctct gattctgtgc ggattgaatc ttgttgagaa gtcattgttg aagacacaaa gaaggcttga agattacggt tccgttaaac cagacaaacg ccgttaccaa aagttccatg ctttccagga ccaccaccaa ggagtttcgt ggacgatttt accgttttac cctccaccgg

Lys Cys Wing Gln Arg Tyr Trp 140 Pro Val Gly Gly Wing Gly Arg Arg 155 160 Arg His Ile Thr Ile Ser Glu 170 175 Gly Leu Gln Wing Asn Thr Arg 190 Gln His Val Wing Ala Pro 205 Asp Gln Lys Val Ser Asn Gly 220 Asp Gln Arg Read Le Val Wing Arg 235 240 Gly Be Ser Val Thr Thr Be 250 255 Wing Gln Be Gly Ser Val Val 270 Asn Asn Met Asn Gly Tyr Wing 285 Thr Trp Asn Pro Wing Met Pro 300 Tyr Pro Met Pro Phe Tyr Pro 315 320 His Gln Be Ser Be Pro Ile 330 335 Pro Thr Read Gly Lys His Pro 350 Asn Glu Thr Glu Arg Lys Gln 365 Thr Read Le Arg Ile Asp Asp Pro 380 Thr Thr Read Gly Ile Lys Asn 395 400 Phe Lys Gly Phe Asp His Lys 410 415 Glu Asn Be Pro Val Valu Be 430 His Asn Phe His Glu Gln Ile

145

ctttacctga ctcagattcc gtgattccgg agactgataa ctaatgatgt caaagacgaa ctgataagat acaactataa ctggtggaac cttctcatta gaactgatct tttgtgaatc ctgtattgca aagaagctgg cttggggag

gcttggtgtt tgttcggtta tttaggacga aaaggaagaa tactactact tgaagagtca tctaccgtgt tgttaaccaa gatgaggaat taaccgtcat tcaacatcct tatggcttct agaacccaat aacagtcagc cgggccgg cgggccgg

60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 gtttcaccgg gggcatggaa cagcttcaca tggatgccac aacccaattc accatctggt 1020 tccaatccaa attctcctac actaggtaaa cattcacgtg acgagaacgc tgctgaacca 1080 ggaaccgctt ttgatgaaac cgagtcactt ggtagggaga aaagcaaacc cgagagatgc 1140 ttgtgggttc ccaagacgct gaggattgat gatccagagg aagctgctaa aagttccatc 1200 tgggaaacat tagggatcaa aaaagacgaa aatgcggata ctttcggagc tttcagatca 1260 tcaaccaaag aaaaaagcag tctttctgaa ggaagacttc cgggaagaag accggagttg 1320 caagcgaatc ctgctgctct ttctaggtca gcaaacttcc atgagagctc atag 1374

<210> 255 <211> 457 <212> PRT

<213> Arabidopsis thaliana <400> 255

Met Wing Asp Pro Wing Ile Lys Leu Phe Gly Lys Thr Ile Pro Leu Pro 1 5 10 15

Glu Leu Gly Val Val Asp Be Ser Ser Ser Ser Tyr Thr Gly Phe Leu Thr

20 25 30

Glu Thr Gln Ile Pro Val Arg Read Asp Be Cys Thr Gly Asp Asp

35 40 45

Asp Asp Glu Glu Met Gly Asp Being Gly Leu Gly Arg Glu Glu Gly Asp

50 55 60

Asp Val Gly Asp Gly Gly Gly Glu Be Glu Thr Asp Lys Lys Glu Glu 65 70 75 80

Lys Asp Be Glu Cys Gln Glu Glu Be Read Arg Asn Glu Be Asn Asp

85 90 95

Val Thr Thr Thr Thr Being Gly Ile Thr Glu Lys Thr Glu Thr Thr Lys

100 105 110

Wing Wing Lys Thr Asn Glu Glu Being Gly Gly Wing Wing Cys Being Gln Glu

115 120 125

Gly Lys Read Lys Lys Pro Asp Lys Ile Read Cys Pro Arg Cys Asn

130 135 140

Being Met Glu Thr Lys Phe Cys Tyr Tyr Asn Asn Tyr Asn Val Asn Gln 145 150 155 160

Pro His Arg Phe Cys Lys Lys Cys Gln Arg Tyr Trp Thr Wing Gly Gly

165 170 175

Thr Met Arg Asn Val Pro Val Gly Ala Gly Arg Arg Lys Asn Lys Ser

180 185 190

Pro Wing Be His Tyr Asn Arg His Val Be Ile Thr Be Wing Glu Wing

195 200 205

Met Gln Lys Val Wing Arg Thr Asp Read Gln His Pro Asn Gly Wing Asn

210 215 220

Leu Leu Thr Phe Gly Be Asp Be Val Leu Cys Glu Be Met Wing Be 225 230 235 240

Gly Leu Asn Leu Val Glu Lys Ser Leu Leu Lys Thr Gln Thr Val Leu

245 250 255

Gln Glu Pro Asn Glu Gly Read Lys Ile Thr Val Pro Read Asn Gln Thr

260 265 270

Asn Glu Glu Wing Gly Thr Val Ser Pro Leu Pro Lys Val Pro Cys Phe

275 280 285

Gly Pro Pro Pro Pro Thr Trp Pro Tyr Wing Trp Asn Gly Val Ser Trp

290 295 300

Thr Ile Leu Pro Phe Tyr Pro Pro Pro Tyr Wing Trp Ser Cys Pro Gly 305 310 315 320

Val Ser Pro Gly Wing Trp Asn Be Phe Thr Trp Met Pro Gln Pro Asn

325 330 335

Be Pro Be Gly Be Asn Pro Asn Be Pro Thr Read Gly Lys His Be

340 345 350

Arg Asp Glu Asn Wing Glu Pro Wing Gly Thr Wing Phe Asp Glu Thr Glu

355 360 365

Be Gly Gly Arg Glu Lys Be Gly Lys Pro Glu Arg Cys Read Trp Val Pro

370 375 380

Lys Thr Leu Arg Ile Asp Asp Pro Glu Glu Wing Wing Lys Ser Ser Ile 385 390 395 400

Trp Glu Thr Read Gly Ile Lys Lys Asp Glu Asn Wing Asp Thr Phe Gly 405

Wing Phe Arg Ser Be Thr Lys Glu 420

Leu Pro Gly Arg Leu Pro Glu Leu

435

440

Arg Ser Ala Asn Phe His Glu Ser

450 455

<210> 256 <211> 57 <212> DNA

<213> Artificial sequence <220>

<223> initiator: prm07303 <400> 256

ggggacaagt ttgtacaaaa aagcaggctt <210> 257 <211> 53 <212> DNA

<213> Artificial sequence <220>

<223> initiator: prm07304 <400> 257

ggggaccact ttgtacaaga aagctgggtc

<210> 258

<211> 654

<212> DNA

<213> Oryza sativa

<400> 258

cttctacatc ggcttaggtg tagcaacacg ttattttaca aaaatataaa atagatcagt ttattgtaaa gttctacaaa gctaatttaa aaacaagagt gtcaatggaa caatgaaaac attgaaatta tataattcaa agagaataaa gcattaccaa aatatatata gcttacaaaa ccttatcaca ttgacacata aagtgagtga atcatgtata tatgatagcc acaaagttac gtgcacctaa cagaatatcc aaataatatg aaaactaaca ctctaaagca accgatggga aatcctcatc atccttcacc acaattcaaa <210> 259 <211> 1828 <212> DNA <213> Oryza sativa <400> 259

gcttgagtca tagggagaaa acaaatcgat taccggcaaa aaaagtagta ctggtttata tccggcttaa tgcttttctt ttgtcacata cttctgccat cccattatat agcaactcaa ctagacatgt taaggctgag ttgggcagtc gcgcacatac ttttcaaact actaaatggt gttgctttaa aaaattatat tgatccattt tcacgtgtta aaagaccgct ccgttttgcg accgaacaca gcctaaatct tgttgtctag tctaagttac tatatactga gatgaataga gatgaagtta ctactagctg cgtttgggag agcacgtgat taattaagta ctagtttaaa ttaagtaact ctcctataga aaacttttac atgtcagtaa aaaataagag agtagaagtt gtatacagtt ataaactatt ccctctgttc catgtaccag taccatgaat cgaatccaga aatatatcac atctgctcta aatatcttat tctgcccgtt gctaagcaca cgccaccccc cgataattga atggaacttc cacattcaga

410 Lys Ser Ser 425

Gln Wing Asn Ser

415

Read Ser Glu Gly Arg 430

Pro Wing Wing Leu Ser 445

aaacaatgat gatggagact agagatc

atatgtaact ctaaatctgt tca

actttattat ccctcaccac aagttattgc catatgacat tccacatagc catgacaagc tgagtcataa tttgatgatg actcacttag aagcatctat tattatagtt

catgttag

57

53

tattattatt attattatta 60 aagtagagca agttggtgag 120 attaacttat ttcatattac 180 actataattt tgtttttatt 240 cgtaaagttc tacatgtggt 300 ttagtttgaa aaattgcaat 360 tattattttc tttgctaccc 420 atatcaaaga acatagagagagagagagagagata40ag 600

tcttttccct ccatctctct 60 gattctttaa ttatgtgaga 120 caacaattgc catatattca 180 atatatcccc tattactaat 240 acccaccacc ttcgtttttc 300 aaaatatttt caatacaaaa 360 aatagctaat acttaattaa 420 ataggttcac atcctgcatt 480 ctggatatat taaatcatgt 540 ttagacccac cttaagtctt 600 aaaaaaaagt attagccatt 660 aataaattaa tatgattctc 720 cgtttaatag tttggaaaat 780 gaaaaagaat tgttttagta 840 gggattatgg atggattcga 900 atgcatattt attctactat 960 ggagactgtc gctatgtttt 1020 cgcctctggc cttcttgcca 1080th gaccgtcgac tccaagtgct 1140th ttgcacaaaa caactccggc ctcccggcca ccagtcacac gactcacggc actaccaccc 1200th ctgactccct gaggcggacc tgccactgtt ctgcatgcga agctatctaa aattctgaag 1260th caaagaaagc acagcacatg ctccgggaca cgcgccaccc ggcggaaaag ggctcggtgt 1320th ggcgatctca cagccgcata tcgcatttca caagccgccc atctccaccg gcttcacgag 1380th gctcatcgcg gcacgaccgc gcacggaacg cacgcggccg acccgcgcgc ctcgatgcgc 1440th gagcccatcc gccgcgtcct ccctttgcct ttgccgctat cctctcggtc gtatcccgtt 1500th tctctgtctt ttgctccccg gcgcgcgcca gttcggagta ccagcgaaac ccggacacct 1560 ggt acacctc cgccggccac aacgcgtgtc ccccctacgt ggccgcgcag cacatgccca 1620 tgcgcgacac gtgcacctcc tcatccaaac tctcaagtct caacggtcct ataaatgcac 1680 ggatagcctc aagctgctcg tcacaaggca agaggcaaga ggcaagagca tccgtattaa 1740 ccagcctttt gagacttgag agtgtgtgtg actcgatcca gcgtagtttc agttcgtgtg 1800 ttggtgagtg attccagcca agtttgcg 1828

<210> 260 <211> 1243 <212> DNA <213> Oryza sativa <400> 260

aaaaccaccg agggacctga tctgcaccgg ttttgatagt tgagggaccc gttgtgtctg 60

gttttccgat cgagggacga aaatcggatt cggtgtaaag ttaagggacc tcagatgaac 120 ttattccgga gcatgattgg gaagggagga cataaggccc atgtcgcatg tgtttggacg 180 gtccagatct ccagatcact cagcaggatc ggccgcgttc gcgtagcacc cgcggtttga 240 ttcggcttcc cgcaaggcgg cggccggtgg ccgtgccgcc gtagcttccg ccggaagcga 300 gcacgccgcc gccgccgacc cggctctgcg tttgcaccgc cttgcacgcg atacatcggg 360 atagatagct actactctct ccgtttcaca atgtaaatca ttctactatt ttccacattc 420 atattgatgt taatgaatat agacatatat atctatttag attcattaac atcaatatga 480 atgtaggaaa tgctagaatg acttacattg tgaattgtga aatggacgaa gtacctacga 540 tggatggatg caggatcatg aaagaattaa tgcaagatcg tatctgccgc atgcaaaatc 600 ttactaattg cgctgcatat atgcatgaca gcctgcatgc gggcgtgtaa gcgtgttcat 660 ccattaggaa gtaaccttgt cattacttat accagtacta catactatat agtattgatt 720 tcatgagcaa atctacaaaa ctggaaagca ataagaaata cgggactgga aaagactcaa 780 cattaatcac caaatatttc gccttctcca gcagaatata tatctctcca tcttgatcac 840 tgtacacact gacagtgtac gcataaacgc agcagccagc ttaactgtcg tctcaccgtc 900 gcacactggc cttccatct c aggctagctt tctcagccac ccatcgtaca tgtcaactcg 960 gcgcgcgcac aggcacaaat tacgtacaaa acgcatgacc aaatcaaaac caccggagaa 1020 gaatcgctcc cgcgcgcggc ggcgacgcgc acgtacgaac gcacgcacgc acgcccaacc 1080 ccacgacacg atcgcgcgcg acgccggcga caccggccgt ccacccgcgc cctcacctcg 1140 ccgactataa atacgtaggc atctgcttga tcttgtcatc catctcacca ccaaaaaaaa 1200 aaggaaaaaa aaacaaaaca caccaagcca aataaaagcg ACA 1243

<210> 261 <211> 8 <212> PRT

<213> Artificial sequence <220>

<223> reason 1 <220>

<221> UNSAFE <222> (2) .. (3)

<223> Xaa can be any naturally occurring amino acid <400> 261

Phe Xaa Xaa Lys Tyr Asn Phe Asp 1 5

<210> 262 <211> 8 <212> PRT

<213> Artificial sequence <220>

<223> reason 2 <220>

<221> VARIANT <222> (1) .. (1) <223> / replace = "Read" <220>

<221> UNSAFE <222> (3) .. (3)

<223> Xaa can be any naturally occurring amino acid <400> 262

Pro Leu Xaa Gly Arg Tyr Glu Trp

1 5

<210> 263

<211> 10

<212> PRT

<213> Artificial sequence <220>

<223> reason 3 <220>

<221> UNSAFE <222> (2) .. (2)

<223> Xaa can be any naturally occurring amino acid <220>

<221> VARIANT <222> (4) .. (4) <223> / replace = "Glu" <220>

<221> UNSAFE <222> (7) .. (9)

<223> Xaa can be any naturally occurring amino acid <400> 263

Glu Xaa Glu Asp Phe Phe Xaa Xaa Xaa Glu 15 10

<210> 264 <211> 8 <212> PRT

<213> Artificial sequence <220>

<223> reason 4 <220>

<221> UNSAFE <222> (2) .. (2)

<223> Xaa can be any naturally occurring amino acid <400> 264

Tyr Xaa Gln Read Arg Be Arg Arg

1 5

<210> 265

<211> 9

<212> PRT

<213> Artificial sequence <220>

<223> reason 5 <220>

<221> VARIANT

<222> (5) .. (5)

<223> / replace = "Ile"

<220>

<221> VARIANT <222> (6) .. (6) <223> / replace = "Arg" <220>

<221> UNSAFE <222> (8) .. (8)

<223> Xaa can be any naturally occurring amino acid <220>

<221> VARIANT

<222> (9) .. (9)

<223> / replace = "Arg"

<400> 265

Met Gly Lys Tyr Met Lys Lys Xaa Lys <210> 266 <211> 8 <212> PRT

<213> Artificial sequence <220>

<223> reason 6 <220>

<221> UNSAFE <222> (2) .. (2)

<223> Xaa can be any naturally occurring amino acid <400> 266

Ser Xaa Gly Val Arg Thr Arg Wing

1 5

<210> 267

<211> 585

<212> DNA

<213> Oryza sativa

<400> 267

atgggcaagt acatgcgcaa ggccaaggtg gtggtctccg gcgaggtggt ggccgccgcc gtcatggagc tcgccgcggc gccgctcggg gtgcgcaccc gcgcccgctc cctcgcgctg cagaagaggc agggcgggga gtacctcgag ctcaggagcc gcaggctcga gaagctccct cctcccccgc cgccgccgcc gaggaggagg gcgacggctg cggctgcgac tgctgatgcg acggcgacgg agagcgcgga ggcggaggtg tcgttcgggg gggagaacgt cctcgagctg gaggccatgg aaaggaatac cagggagacg acaccttgca gcttgatcag ggaccccgat acgattagca cccctggatc taccacaagg cgcagccact cgagttctca ttgcaaggtg caaacacccg tgcgccacaa cattattcca gcatcagcag agctggaagc gttcttcgcc gccgaagagc aacggcaacg acaggctttc atcgacaagt ataactttga tcctgtgaat gactgccctc ttcccggccg atttgaatgg gtcaagctag actga <210> 268 <211> 194 <212> PRT

<213> Oryza sativa <400> 268

Met Gly Lys Tyr Met Arg Lys Wing Lys Val Val Val Ser Gly Glu Val 1 5 10 15 Val Wing Wing Wing Val Wing Met Glu Leu Wing Wing Pro Wing Leu Gly Val Arg 20 25 30 Thr Arg Wing Arg Be Leu Wing Leu Gln Lys Arg Gln Gly Gly Glu Tyr 35 40 45 Leu Glu Leu Arg Be Arg Arg Leu Glu Lys Leu Pro Pro Pro Pro 50 55 60 Pro Pro Arg Arg Wing Thr Wing Wing Wing Wing Wing Wing Wing Wing 65 Wing 75 Wing Wing Thr Wing Wing Glu Ser Wing Glu Wing Glu Val Val Phe Gly Gly Glu Asn 85 90 95 Val Leu Glu Leu Glu Wing Met Glu Arg Asn Thr Arg Glu Thr Thr Pro 100 105 110 Cys Ser Leu Ile Arg Asp Pro Asp Thr Ile Ser Thr Pro Gly Ser Thr 115 120 125 Thr Arg Arg Be His Be Be His Be Cys Lys Val Gln Thr Pro Val 130 135 140 Arg His Asn Ile Ile Pro Wing Be Wing Glu Leu Glu Wing Phe Phe Wing 145 150 155 160 Wing Glu Glu Gln Arg Gln Arg Gln Wing Phe Ile Asp Lys Tyr Asn Phe 165 170 175 Asp Pro Val Asn Asp Cys Pro the Gly Arg Phe Glu Trp Val Lys 180 185 190 Leu Asp

<211> 573 <212> DNA <213> Zea mays <400> 269

atgggcaagt acatgcgcaa ggccaaggct tccagcgagg ttgtcatcat ggatgtcgcc 60

gccgctccgc tcggagtccg cacccgagcg cgcgccctcg cgctgcagcg tctgcaggag 120 caacagacgc agtgggaaga aggtgctggc ggcgagtacc tggagctaag gaaccggagg 180 ctcgagaagc tgccgccgcc gccggcgacc actaggaggt cgggcgggag gaaagcggca 240 gccgaggccg ccgcaactaa ggaggctgag gcgtcgtacg gggagaacat gctcgagttg 300 gaggccatgg agaggattac cagggagacg acgccttgca gcttgattaa cacccagatg 360 actagcactc ctgggtccac gagatccagc cactcttgcc accgcagggt gaacgctcct 420 ccggtgcacg ccgtcccaag ttctagggag atgaatgagt acttcgctgc cgaacagcga 480 cggcaacagc aggatttcat tgacaagtac aacttcgatc ctgcaaacga ctgccctctc 540 ccaggcaggt ttgagtgggt gaagctagac tga 573

<210> 270 <211> 190 <212> PRT <213> Zea mays <400> 270

Met Gly Lys Tyr Met Arg Lys Wing Lys Wing Ser Ser 1 5 10

Met Asp Val Wing Pro Wing Wing Read Gly Val Arg Thr

20 25

Leu Ala Leu Gln Arg Leu Gln Glu Gln Gln Thr Gln

35 40

Wing Gly Gly Glu Tyr Leu Glu Leu Arg Asn Arg Arg

50 55 60

Pro Pro Pro Pro Wing Thr Thr Arg Arg Be Gly Gly 65 70 75

Wing Glu Wing Wing Wing Thr Thr Lys Wing Glu Wing Wing Ser

85 90

Met Leu Glu Leu Glu Wing Met Glu Arg Ile Thr Arg

100 105

Cys Be Read Ile Asn Thr Gln Met Thr Be Thr Pro

115 120

Be Gly His Be Cys His Arg Arg Val Asn Ala Pro 130 135 140

Val Pro Be Ser Arg Glu Met Asn Glu Tyr Phe Wing 145 150 155

Arg Gln Gln Gln Asp Phe Ile Asp Lys Tyr Asn Phe

165 170

Asp Cys Pro Read Pro Gly Arg Phe Glu Trp Val Lys

180 185

<210> 271 <211> 755 <212> DNA

<213> Triticum aestivum <400> 271

atgggcaagt acatgcgcaa gcccaaggtc tccggcgagg tggccgtcat ggaggtcgcc 60

gccgcgccgc taggggtccg cacccgcgca cgagcgctcg cgatgcagag gcagccgcag 120 ggggcggcgg tggccaagga ccagggggag tacctggagc tcaggagtcg gaagctcgag 180 aagctgcccc cgccgccgcc ggcggcgagg aggagggcgg ccgcggcgga gcgtgtcgag 24 0 gccgaggccg aggccgacga ggtgtccttc ggtgagaacg tgctcgagtc ggaggccatg 300 gggaggggta ccagggagac gacgccctgc agcttgatta gggactcggg aacgataagc 360 actcctggat ccacaacaag accgagccac tcgaattccc atcgcagggt gcaagctcca 420 gcgcgccata ttattccatg ttcagcagag atgaatgagt tcttctctgc tgcggagcaa 480 ccgcaacagc aagccttcat tgacaagtac aactttgatc ctgtgaacga ctgtcctctc 540 ccaggccgat acgagtgggt gaagctagac tgataattct ccaggaagga gagcaccatg 600 tacttctccg ctccctccac cttagcgtcg tggtaaaggc cgccccgtcg tgtagctttg 660 tttccgttgt aaaaagaata gttagctgta gtagcctcaa tggcgttaca tacagagtaa 720 755 tgctgattac acctaatcct caaaccatgt acgtt

<210> 272 <211> 190 <212> PRT

<213> Triticum aestivum

Glu Val Val Ile 15 Arg Wing Arg Wing 30 Trp Glu Glu Gly 45 Leu Glu Lys Leu Arg Lys Wing 80 Tyr Gly Glu Asn 95 Glu Thr Thr Pro 110 Gly Ser Thr Arg 125 Pro Val His Wing Glu Gln Arg 160 Asp Pro Wing Asn 175 Leu Asp 190 <400> 272

Met Gly Lys Tyr Met Arg Lys Pro Lys Val Ser Gly Glu Val Wing Val 15 10 15

Met Glu Val Wing Ward Wing Pro Wing Read Gly Val Arg Thr Arg Wing Arg Wing

20 25 30

Leu Met Met Gln Arg Gln Pro Gln Gly Wing Val Wing Wing Lys Asp Gln

35 40 45

Gly Glu Tyr Leu Glu Leu Arg Be Arg Lys Leu Glu Lys Leu Pro

50 55 60

Pro Pro Pro Wing Arg Wing Arg Arg Wing Wing Wing Wing Wing Glu Arg Val Glu 65 70 75 80

Glu Wing Glu Wing Asp Glu Wing Val Ser Phe Gly Glu Asn Val Leu Glu

85 90 95

Be Glu Ala Met Gly Arg Gly Thr Arg Glu Thr Thr Pro Cys Ser Leu

100 105 110

Ile Arg Asp To Be Gly Thr Ile Arg To Be Gly Thr Thr

115 120 125

Be His Be Asn Be His Arg Arg Val Gln Pro Wing Arg His Ile

130 135 140

Ile Pro Cys Be Wing Glu Met Asn Glu Phe Phe Be Wing Glu Gln 145 150 155 160

Pro Gln Gln Gln Wing Phe Ile Asp Lys Tyr Asn Phe Asp Pro Val Asn

165 170 175

Asp Cys Pro Leu Pro Gly Arg Tyr Glu Trp Val Lys Leu Asp 180 185 190

<210> 273

<211> 666

<212> DNA

<213> Oryza sativa

<400> 273

atggggaagt acatgcggaa ggggaaggtg tcgggggagg tggcggtgat ggaggtgggc 60

ggggcgctgc tcggcgtccg cacccgctcc cgcacgctcg cgctgcagcg gacgacctcg 120 tcgcagaagc cgccggagaa gggggagggg gaccccggtg cgggcgcggg cgcgggggcg 180 gagtacctcg agctcaggag ccggaggctc gagaagccgc ctccgcacac gccgccggcc 240 aaggagaagg agaccgccag gagggcttcc gccgccgccg ccgccgccgt gaggatgccg 300 gcggcgccgc aagcggccga ggagttcgag gcggaggtcg aggtgtcctt cggcgacaac 360 gttcttgacc tcgacggcga cgccatggag aggagtacca gggagacaac gccttgcagt 420 ttaattagga gctcagaaat gataagcacc cctggctcca caactaaaac caacacctcg 480 atcagttccc ggcgcagaat ggagacctct gtttgtcgtt acgttccgag ttctcttgag 540 atggaagagt tctttgcagc tgctgaacaa cagcaacatc aggctttcag agaggtc 600 aacttctgtc ctgtgaacgg cgggggggggg 6

<210> 274 <211> 221 <212> PRT

<213> Oryza sativa <400> 274

Met Gly Lys Tyr Met Arg Lys Gly Lys Val Ser Gly Glu Val Wing Val 15 10 15

Met Glu Val Gly Gly Wing Read Leu Gly Val Arg Thr Arg Be Arg Thr

20 25 30

Read Ala Read Le Gln Arg Thr Thr Be Being Gln Lys Pro Pro Glu Lys Gly

35 40 45

Glu Gly Asp Pro Gly Wing Gly Wing Gly Wing Gly Wing Glu Tyr Leu Glu

50 55 60

Read Arg Be Arg Arg Read Le Glu Lys Pro Pro His Thr Pro Pro Wing 65 70 75 80

Lys Glu Lys Glu Thr Wing Arg Arg Wing Be Wing Wing Wing Wing Wing Wing

85 90 95

Val Arg Met Pro Wing Pro Wing Gln Wing Glu Wing Glu Phe Glu Wing Glu

100 105 110

Val Glu Val Ser Phe Gly Asp Asn Val Read Asp Leu Asp Read Asp Gly Asp Wing 115 120 125 Met Glu Arg Be Thr Arg Glu Thr Thr Pro Cys

130 135

Be Glu Met Ile Be Thr Pro Gly Be Thr Thr 145 150 155

Ile Ser Be Arg Arg Arg Met Glu Thr Be Val

165 170

Being Being Read Glu Met Glu Glu Phe Phe Wing Wing

180 185

His Gln Phe Arg Wing Glu Arg Tyr Asn Phe Cys

195 200

Pro Leu Pro Gly Arg Tyr Glu Trp Thr Arg Leu

210 215

<210> 275 <211> 642 <212> DNA <213> Zea mays <400> 275

atggggaagt acatgcgcaa gggcaaggtg tccggggagg ggcggcgcgc tgctcggcgt ccgcacccgc tcccgcacgc aggccgctcg acaagggcga cgcggaggac gccgccgcgg cggaggctcg agaagccgca caaggagcat ccgtcgccgc ggcgccggga ggaaggccgc cgccgccgcc gcggtgcagc gtcgaggtcg aggtctcgtt cggggacaac gtgcttgact accagagaga caacaccgtg cagcctgatt aggaacccag tccacaacta aaagcaaaac cagcagcaac tcgacgactt accccgagct gccggttcat accgagctcg ctcgagatgg gagcaacagg agcagcatag cttcagggag aagtacaact cctctccctg gccggtacga atgggcgagg ctagactgct <210> 276 <211 > 213 <212> PRT <213> Zea mays <400> 276

Met Gly Lys Tyr Met Arg Lys Gly Lys Val Ser 15 10

Met Glu Val Pro Gly Gly Wing Read Leu Gly Val

20 25

Thr Leu Wing Leu Gln Arg Wing Gln Arg Pro Leu

35 40

Glu Asp Wing Wing Wing Glu Tyr Leu Glu Leu Arg

50 55

Lys Pro His Lys Glu His Pro Ser Pro Pro Wing 65 70 75

Gly Wing Gly Arg Lys Wing Wing Wing Wing Wing Wing

85 90

Met Gln Asp Glu Val Glu Val Glu Val Ser Phe

100 105

Asp Read Asp Thr Met Glu Arg Be Thr Arg Glu

115 120

Read Ile Arg Asn Pro Glu Met Ile Be Thr Pro

130 135

Be Lys Thr Be Ser Asn Be Thr Thr Be Arg 145 150 155

Thr Pro Be Cys Arg Phe Ile Pro Be Ser Read

165 170

Phe Ser Wing Ala Glu Gln Gln Glu Gln His Ser

180 185

Asn Phe Cys Pro Val Asn Asp Cys Pro Leu Pro

195 200

Arg Wing Read Asp Cys 210 <210> 277 <211> 642

Ser Leu Ile Arg Ser 140

Lys Thr Asn Thr Ser 160

Cys Arg Tyr Val Pro 175

Glu Gln Wing Gln Gln 190

Pro Val Asn Asp Cys

205 Asp Cys 220

tcgccgtcat tcgcgctgca agtacctcga ccgcgaccgc acgtgctgat tggacaccat agatgataag cccgccgcag aggagttctt tctgtcccgt ag

ggaggtaccc gcgcgcgcag gctcaggagc gaccaagagg gcaggacgag ggaaaggagt caccccagga aacggaggaa ctcggcggcc gaacgactgt

60 120 180 240 300 360 420 480 540 600 642

Gly Glu

Arg thr

Asp Lys

45 Ser Arg 60

Thr wing

Val gln

Gly asp

Thr Thr 125 Gly Ser 140

Arg Arg

Glu Met

Phe arg

Gly Arg 205

Val

Arg

30

Gly

Arg

Thr

His

Asn 110 pro

Thr

Thr

Glu

Glu 190 Tyr

Val Wing 15

To be Arg

Asp Wing

Read Glu

Lys Arg 80 Val Leu 95

Val leu

Cys ser

Thr lys

Glu Glu 160 Glu Phe 175

Lys Tyr Glu Trp <212> DNA

<213> Sorghum bicolor <400> 277

atggggaagt acatgcgcaa gggcaaggtg tccggggagg tcgccgtcat ggcggcgcgc tgctcggcgt ccgcacccgc tcccgcacgc tcgcgctgca aggccgctcg acaagggcga cgccgaggac gccgctgcgg agtacctcga cggaggctcg agaagccgca caaggacccg ttaccgccgc cgtctgcgcc aggggcgccg ggaggaaggt cgccaccgcc gccgccgccg ccgcggcgcc gcggaggacg acgtcgaggt ctccttcggc gagaacgtgc tcgacttcga aggagtacca gagagacaac accgtgcagt ttgattagga acccagagat ccaggatcca caactaagag taaaaccagc aactcgatga cctcccgtcg acctcaatct gccgtttcat accaagttcg catgagatgg aagagttctt gaaaaacagg agcagcaaag cttcagggag aagtataact tctgtcctgt cctcttccgg gtcggtatga atgggcgagg ctagactgct ag <210> 278 <211> 213 <212> PRT

<213> Sorghum bicolor <400> 278

Met Gly Lys Tyr Met Arg Lys Gly Lys Val Ser Glj 1 5 10

Met Glu Val Pro Gly Gly Wing Read Leu Gly Val Arc

20 25

Thr Leu Wing Leu Gln Arg Wing Gln Arg Pro Leu Asj

35 40

Glu Asp Wing Wing Wing Glu Tyr Leu Glu Leu Arg Se: 50 55 60

Lys Pro His Lys Asp Pro Read Pro Pro Pro Ser Ale 65 70 75

Arg Gly Wing Gly Arg Lys Val Wing Thr Wing Wing Alc Wing

85 90

Pro His Gly Leu Wing Glu Asp Asp Val Glu Val Sei

105

ggaggtcccc gcgcgcgcag gctcaggagc tgcgaccaag gcacgggctg cgccatggaa gataagcacc cagaatggaa ctcagcagct gaacgactgt

100 Val Leu Asp 115 Phe Asp Wing Cys Ser 130 Leu Ile Arg Asn

120

135

140

155

170

185

200

Glu Val Wing Val 15 Thr Arg Be Arg 30 Lys Gly Asp Wing 45 Arg Arg Leu Glu Pro Wing Thr Lys 80 Wing Wing Wing 95 Phe Gly Glu Asn 110 Glu Thr Thr Pro 125 Pro Gly Be Thr Arg Arg Glu Phe 175 Arg Glu Lys Tyr 190

210 <210> 279 <211> 397 <212> DNA

<213> Saccharum officinarum <220>

<221> misc_feature <222> (342). . (343) <223> η is a, c, g, or t <400> 279

gtcgacccac gcgtccggta cttcgctgct gaacagcgac gacaagtaca actttgatcc tgtaaatgac tgccctctcc aagctagact gattgattca gaggacaaga gagcagcagc ctccaccacc gcagtgttgt ggcagaggcg cataccgtcg taaaaactta gtgttagcct gtagccttaa ttgtcgtgtg tgagttacaa caccctgatc tgatctgatc cctcaactca attctgtaag gaaccttacc cacccttgtt accagtt

60 120 180 240 300 360 420 480 540 600 642

gccaacaaca ggctttcatt 60 caggcaggtt tgaatgggtg 120 atggaactca cctccgctcc 180 tgttagcttt gtttctgttg 240 ttacagtaca aaactgatgc 300 gnngtaaccc ttaacagctt 360 397 <210> 280 <211> <12> 37

<213> Saccharum officinarum <400> 280

Tyr Phe Wing Glu Wing Gln Arg Arg Gln Gln Gln 15 10

Tyr Asn Phe Asp Pro Val Asn Asp Cys Pro Leu

20 25

Trp Val Lys Leu Asp 35

<210> 281

<211> 654

<212> DNA

<213> Oryza sativa

<400> 281

cttctacatc ggcttaggtg tagcaacacg actttattat ttattttaca aaaatataaa atagatcagt ccctcaccac ttattgtaaa gttctacaaa gctaatttaa aagttattgc aaacaagagt gtcaatggaa caatgaaaac catatgacat attgaaatta tataattcaa agagaataaa tccacatagc gcattaccaa aatatatata gcttacaaaa catgacaagc ccttatcaca ttgacacata aagtgagtga tgagtcataa atcatgtata tatgatagcc acaaagttac tttgatgatg gtgcacctaa cagaatatcc aaataatatg actcacttag aaaactaaca ctctaaagca accgatggga aagcatctat aatcctcatc atccttcacc acaattcaaa tattatagtt <210> 282 <211 > 1243 <212> DNA <213> Oryza sativa <400> 282

aaaaccaccg agggacctga tctgcaccgg ttttgatagt gttttccgat cgagggacga aaatcggatt cggtgtaaag ttattccgga gcatgattgg gaagggagga cataaggccc gtccagatct ccagatcact cagcaggatc ggccgcgttc ttcggcttcc cgcaaggcgg cggccggtgg ccgtgccgcc gcacgccgcc gccgccgacc cggctctgcg tttgcaccgc atagatagct actactctct ccgtttcaca atgtaaatca atattgatgt taatgaatat agacatatat atctatttag atgtaggaaa tgctagaatg acttacattg tgaattgtga tggatggatg caggatcatg aaagaattaa tgcaagatcg ttactaattg cgctgcatat atgcatgaca gcctgcatgc ccattaggaa gtaaccttgt cattacttat accagtacta tcatgagcaa atctacaaaa ctggaaagca ataagaaata cattaatcac caaatatttc gccttctcca gcagaatata tgtacacact gacagtgtac gcataaacgc agcagccagc gcacactggc cttccatctc aggctagctt tctcagccac gcgcgcgcac aggcacaaat tacgtacaaa acgcatgacc gaatcgctcc cgcgcgcggc ggcgacgcgc acgtacgaac ccacgacacg atcgcgcgcg acgccggcga caccggccgt ccgactataa atacgtaggc atctgcttga tcttgtcatc aaggaaaaaa aaacaaaaca caccaagcca aataaaagcg <210> 283 <211> 315 <212> DNA

<213> Nicotiana tabacum <400> 283

ctgccattct ttagagggga tgcttgttta agaacaaaaa ccaagtcatt gcgtattttt ttaaaaatat ttgttccttc actttatggt tctttgtatt ctggctttgc tgtaaatcgt gagatcagtagtagtagtagtagta

Phe Ile Wing Asp Lys 15

Pro Gly Arg Phe Glu 30

tattattatt attattatta 60 aagtagagca agttggtgag 120 attaacttat ttcatattac. 180 actataattt tgtttttatt 240 cgtaaagttc tacatgtggt 300 ttagtttgaa aaattgcaat 360 tattattttc tttgctaccc 420 atatcaaaga acatttttag 480 atcataatag agcatcaagt 540 aaatagacaa gcacaatgaa 600 gaagcatagt agga 654

tgagggaccc gttgtgtctg 60 ttaagggacc tcagatgaac 120 atgtcgcatg tgtttggacg 180 gcgtagcacc cgcggtttga 240 gtagcttccg ccggaagcga 300 cttgcacgcg atacatcggg 360 ttctactatt ttccacattc 420 attcattaac atcaatatga 480 aatggacgaa gtacctacga 540 tatctgccgc atgcaaaatc 600 gggcgtgtaa gcgtgttcat 660 catactatat agtattgatt 720 cgggactgga aaagactcaa 780 tatctctcca tcttgatcac 840 ttaactgtcg tctcaccgtc 900 ccatcgtaca tgtcaactcg 960 aaatcaaaac caccggagaa 1020 gcacgcacgc acgcccaacc 1080 ccacccgcgc cctcacctcg 1140 catctcacca ccaaaaaaaa 1200 aca 1243

atatatcact ttcttttgtt 60 gtatatttcg agcttcaatc 120 agctaacctt cttcctagca 180 aattacatat taccaccaca 240 gattcatttt gcaaaatttc 300 caggatattg acaac 315

<210> 284 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> Primer: prm00472 <400> 284

ggggacaagt ttgtacaaaa aagcaggctt cacaatgggc aagtacatgc gca 53

<210> 285 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> Primer: prm00473 <400> 285

ggggaccact ttgtacaaga aagctgggtg gagcagagag gtccatggtg ccc 53

Claims (206)

1. Isolated SYR protein selected from the group, characterized in that it consists of: (a) a polypeptide as given in SEQ ID NO 44; (b) a polypeptide with an amino acid sequence having at least, in ascending order of preference, 85%, 86%, 87%, 88%, - 89%, 90%, 91%, 92%, 93%, 94 %, 95%, 96%, 97%, 98% or 99% or more of sequence identity with an amino acid sequence as given in SEQ ID NO 44; (c) a derivative of a polypeptide as defined in (a) or b) an isolated nucleic acid sequence comprising: (a) a nucleic acid sequence represented by SEQ ID NO: 43, or the complementary ribbon thereof; (b) a nucleic acid sequence encoding the amino acid sequence represented by SEQ ID NO: 44; (c) a nucleic acid sequence capable of hybridizing (preferably under stringent conditions) to a nucleic acid sequence of (a) or (b), whose hybridizing sequence preferably encodes a SYR protein; (d) a nucleic acid which is an allelic variant for nucleic acid sequences according to (a) or (b); (e) a nucleic acid which is an alternative splice variant for nucleic acid sequences according to (a) or (b); (f) a nucleic acid sequence that has 50%, 60%, - 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity with the sequence defined in (a) or (b). A method for increasing seed production and / or increasing plant growth rate over corresponding wild-type plants, which comprises modulating expression in a plant of a nucleic acid encoding a SYR polypeptide or homologue thereof; and optionally selecting plants having improved growth characteristics; provided that said SYR protein or homologue thereof is not the protein of SEQ ID NO: 26. Method according to claim 2, characterized in that said modulated expression is effected by introducing a genetic modification preferably at the site of a gene encoding a SYR polypeptide or a homologue thereof, preferably wherein said genetic modification is effected by a gene. de: T-DNA activation, TILLING, site-directed mutagenesis, homologous recombination or direct evolution. Method for increasing seed production and / or increasing growth rate relative to corresponding wild-type plants, comprising introducing and expressing into a plant a SYR nucleic acid or variant thereof; provided that said SYR protein or homologue thereof is not the protein of SEQ ID NO: 26. A method according to claim 4, characterized in that said nucleic acid encodes a SYR protein homolog of SEQ ID NO: 2, preferably said nucleic acid encodes a SYR protein ortholog or analog of SEQ ID NO: 2. The method of claim 4, wherein said variant is a portion of a SYR nucleic acid or sequence capable of hybridizing to a SYR nucleic acid, whose hybridizing portion or sequence encodes a polypeptide of about 65 to about 200 amino acids, comprising a leucine-rich domain, preceded by the conserved tripeptide motif 1 (one of SEQ ID NO: 6, 7, 8 or 9) and followed by the conserved motif 2 (SEQ ID NO: 10) and preferably also for retained reason 3 (SEQ ED NO: 11). A method according to any one of claims 4 to -6, characterized in that said nucleic acid comprises the conserved motifs of SEQ ID NO: 6, SEQ ID NO: 10 and SEQ ID NO: 11, wherein the motif of SEQ ID NO: 10 is VLAFMPT and wherein the motif of SEQ ID NO: -11 is PYL, preferably wherein said nucleic acid comprises the sequence of SEQ ED NO: 1. Method according to any one of claims 4 to 7, characterized in that said SYR nucleic acid or variant thereof is overexpressed in a plant. Method according to any one of claims 4 to 8, characterized in that said SYR nucleic acid or variant thereof is of plant origin, preferably from a monocotyledonous plant, more preferably from the Poaceae family, most preferably acid. nucleic acid is from Oryza sativa. A method according to any one of claims 4 to 9, characterized in that said SYR nucleic acid or variant thereof is operably linked to a constitutive promoter, preferably wherein said constitutive promoter is a GOS2 promoter or a protein promoter. high mobility. A method according to any one of claims 1 to 10, characterized in that said increased seed yield is selected from: increased total seed weight, increased number of full seeds, seed filling rate or seed index. harvesting, and wherein said increased growth rate comprises at least increased seed production obtained without delay in flowering season. A method according to any one of claims 1 to 13, wherein said plants are grown under stress free conditions, or wherein said plants are grown under abiotic stress conditions, preferably wherein said abiotic stress conditions are conditions. of osmotic stress. Plant cell, characterized in that it is obtainable by a method as defined in any one of claims 1 to 12. Construction, characterized in that it comprises: (i) a nucleic acid SYR or a variant thereof; (ii) one or more control sequences capable of directing expression of the nucleic acid sequence of (i); preferably wherein said control sequence is a constitutive promoter or a High Mobility Group Protein (HMGP) promoter, and optionally (iii) a transcription termination sequence as long as said SYR nucleic acid does not encode the protein of SEQ ID NO: 26. Plant cell, characterized in that it is transformed with a construct as defined in claim 14. Method for producing a transgenic plant having increased yield compared to corresponding wild type plants, characterized in that it comprises: (i). introducing and expressing into a plant or plant cell a SYR nucleic acid or variant thereof; and (ii). cultivating the plant cell under conditions promoting plant growth and development, provided that said SYR nucleic acid or variant thereof does not encode the protein of SEQ ID NO: 26. A method for improving plant growth characteristics over control plants, comprising increasing expression in a plant of a nucleic acid encoding an FG-GAP polypeptide or homologue thereof, and optionally selecting plants having characteristics of improved growth. The method according to claim 17, characterized in that said increased expression is effected by introducing a genetic modification preferably at the site of a gene encoding an FG-GAP polypeptide or a homologue thereof, preferably wherein said genetic modification is effected. by one of: T-DNA activation, TILLING, site-directed mutagenesis, homologous recombination or direct evolution. A method for enhancing growth characteristics over corresponding wild-type plants, which comprises introducing and expressing a FG-GAP nucleic acid or variant thereof into a plant. Method according to claim 19, characterized in that said nucleic acid encodes an FG-GAP protein homolog of SEQ ID NO: 46, preferably said nucleic acid encodes an FG-GAP protein ortholog or analog of SEQ ID NO: 46, preferably wherein said nucleic acid comprises one or more of the conserved motifs of SEQ ID NO: 50 to 52. A method according to claim 19, wherein said variant is a portion of an FG-GAP nucleic acid or sequence capable of hybridizing to an FG-GAP nucleic acid, whose hybridizing portion or sequence encodes a polypeptide comprising a signal peptide, one or more FG-GAP domains, and a transmembrane domain located at the C-terminal half of the protein. A method according to any one of claims -21 to 25, characterized in that said improved growth characteristic is increased yield, preferably increased seed yield, preferably wherein said increased seed yield is selected from: weight. increased total seed, increased number of full seeds or increased harvest index. Plant cell, characterized in that it is obtainable by a method as defined in any one of claims 17 to 22. 24. Construction, characterized in that it comprises: (i) an FG-GAP nucleic acid or variant thereof; (ii) one or more control sequences capable of directing expression of the nucleic acid sequence of (i), preferably wherein said control sequence is a constitutive promoter, preferably a constitutive promoter is a GOS2 promoter; and optionally (iii) a transcription termination sequence as long as said construct is not a pPZP-like gene construct. Plant cell, characterized in that it is transformed with a construct as defined in claim 24. A method for producing a transgenic plant having increased production compared to corresponding wild-type plants, comprising: (a) introducing and expressing into the plant or plant cell an FG-GAP nucleic acid or variant thereof; (b) cultivate the plant cell under conditions promoting plant growth and development. 27. Isolated FG-GAP protein, characterized in that it is selected from the group consisting of: (a) a protein encoded by nucleic acid of SEQ ID NO: 72; (b) a protein comprising a signal sequence, one or more FG-GAP domains, and a transmembrane domain located at the C-terminal half of the protein, wherein said protein comprises at least one of SEQ ID NO: 73 to SEQ ID NO: 76 ; (c) an active fragment of an amino acid sequence as defined in (a) or (b), whose active fragment comprises a signal sequence, one or more FG-GAP domains, and a transmembrane domain located at the C-terminal half of the protein; or an isolated nucleic acid encoding an FG-GAP protein selected from the group consisting of: (i) the nucleic acid as represented by SEQ ID NO: 72; (ii) a nucleic acid encoding a protein as defined in (a) to (c) above; (iii) a nucleic acid sequence capable of hybridizing (preferably under stringent conditions) to a nucleic acid sequence of (i) or (ii) above, whose hybridizing sequence preferably encodes a protein comprising a signal peptide, one or more FG domains -GAP is a transmembrane domain located at the C-terminal half of the protein; (iv) a nucleic acid which is an allelic variant for nucleic acid sequences according to (i) to (iii); (v) a nucleic acid which is an alternative splice variant for nucleic acid sequences according to (i) to (iii); (vi) a portion of a nucleic acid sequence according to any one of (i) to (v) above, which portion preferably encodes a protein comprising a signal peptide, one or more FG-GAP domains, and a transmembrane domain located at the C-terminal half of the protein. 28. Isolated CYP90B protein, characterized in that it is selected from the group consisting of: (i) a protein encoded by nucleic acid of SEQ ID NO: 117; (ii) a protein comprising the following: (i) CYP AaD domains; (ii) an N-terminal hydrophobic anchor domain; (iii) a transition domain; and (iv) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position, and in ascending order of preference at least 85%. %, -87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% identity with the SEQ amino acid sequence ID NO: 118, or an isolated nucleic acid encoding a CYP90B protein, selected from the group consisting of: (i) a nucleic acid as represented by SEQ ID NO: 117; (ii) a nucleic acid encoding a protein as defined in (i) and (ii) above; (iii) a nucleic acid having in ascending order preferably at least 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, -91%, 92%, 93% 94%, 95%, 96%, 97%, 98%, 99% or more nucleic acid identity represented by SEQ ID NO: 117; (iv) a nucleic acid sequence capable of hybridizing under stringent conditions to a nucleic acid sequence of (i) to (iii) above, whose hybridizing sequence encodes a protein comprising (a) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position, and preferably increasing by at least -85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, -98%, 99% or more of sequence identity amino acids of SEQ ID NO: 118; (v) a nucleic acid which is an allelic variant or a splice variant of nucleic acid sequences according to (i) to (iv); (vi) a portion of a nucleic acid sequence according to any one of (i) to (v) above, whose portion encodes a protein comprising: (i) CYP AaD domains; (ii) an N-terminal hydrophobic anchor domain; (iii) a transition domain; and (iv) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position, and preferably ascending at least 85%. %, 87%, 88%, 89%, 90%, -91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% identity with the SEQ amino acid sequence ID NO: 118. 29. A method for increasing plant yield over suitable control plants, which comprises increasing non-constitutive expression in a plant of a nucleic acid encoding a cytochrome P450 (CYP) monooxygenase (CYP) or a homologue thereof, and optionally selecting plants having increased yield, wherein said CYP90B or homologous polypeptide comprises the following: (a) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, allowing an amino acid change at any position. A method according to claim 29, characterized in that said CYP90B polypeptide or homologue thereof further comprises: (i) a sequence with more than 50% identity to SEQ ID NO: 78; and (ii) enzymatic activity of steroid 22-alpha hydroxylase. Method according to claim 29 or 30, characterized in that said increased non-constitutive expression is effected by introducing a genetic modification preferably at the site of a gene encoding a CYP90B polypeptide or a homologue thereof, preferably wherein said genetic modification. is performed by one of: T-DNA activation, TILLING, site-directed mutagenesis, or direct evolution. 32. A method of increasing plant yield over suitable control plants, which comprises introducing and non-constitutively expressing in a plant a nucleic acid CYP90B or variant thereof, preferably wherein said variant is a portion of a nucleic acid. CYP90B, the portion of which encodes a polypeptide comprising: (a) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position, or preferably wherein said variant is a sequence capable of hybridizing to a CYP90B nucleic acid whose hybridizing sequence encodes a polypeptide comprising: (a) CYP AaD domains; (b) an N-terminal hydrophobic anchor domain; (c) a transitional domain; and (d) within domain A, the Phe-Ala-Gly-His-Glu-Thr-Ser-Ser consensus sequence, assuming an amino acid change at any position, or preferably wherein said variant encodes a protein ortholog or paralog CYP90B of SEQ ID NO: 78. A method according to claim 32, characterized in that said CYP90B nucleic acid or variant thereof is of plant origin, preferably from a monocotyledonous plant, still preferably from the Poaceae family, more preferably from the genus Oryza, most preferably from Oryza sativa. A method according to any one of claims 31 to 33, characterized in that said CYP90B nucleic acid or variant thereof is operably linked to a non-constitutive promoter, preferably wherein said non-constitutive promoter is a seed specific promoter. preferably said seed specific promoter is an endosperm specific promoter, still preferably the endosperm specific promoter is a prolamine promoter, preferably wherein said endosperm specific promoter is a rice RP6 prolamine promoter, more preferably the rice promoter specific promoter. endosperm is represented by a nucleic acid sequence substantially similar to SEQ ID NO: 109, most preferably the endosperm specific promoter is as represented by SEQ ID NO: 109, or wherein said non-constitutive promoter is a seed specific promoter, preferably said seed specific promoter is a promo embryo / aleurone specific promoter, still preferably the embryo / aleurone specific promoter is an oleosin promoter, preferably wherein said embryo / aleurone specific promoter is an 18 kDa rice oleosin promoter, more preferably the embryo specific promoter. / aleurone is represented by a nucleic acid sequence substantially similar to SEQ ID NO: 110, most preferably the embryo / aleurone specific promoter is as represented by SEQ ID NO: 110. A method according to any one of claims -29 to 34, characterized in that said increased yield is selected from one or both of: increased total seed yield or increased harvest index (HI) each with respect to each other. to appropriate control plants. A method according to any one of claims -29 to 34, characterized in that said increased yield is selected from one or more of: increased thousand seed weight (TKW), increased seed area or seed length each in relation to appropriate control plants. A method according to any one of claims -29 to 36, characterized in that said plant is a monocotyledonous plant. Plant cell, characterized in that it is obtainable by a method as defined in any one of claims 29 to 37. 39. Plant cell, characterized in that it is transformed with a construct comprising a CYP90B nucleic acid or variant thereof under the control of a RP6 prolamine promoter. 40. A method of producing a transgenic plant having an increased yield over the appropriate control plant, comprising: (a) introducing and non-constitutively introducing into a plant or plant cell a CYP90B nucleic acid or variant thereof; (b) cultivate the plant cell under conditions promoting plant growth and development. -97. Transgenic plant, characterized in that it has increased yield over the appropriate control plant, said increased yield resulting from a nucleic acid CYP90B or a variant thereof introduced and expressed non-constitutively in said plant. 41. A method of increasing plant seed count relative to suitable control plants, which comprises preferably increasing expression in the bud apical meristem tissue of a plant of a nucleic acid encoding a CDC27 polypeptide having at least one TPR domain. inactive in the NH2 terminal region of the polypeptide, and optionally select plants having increased seed numbers. Method according to claim 41, characterized in that said increased expression is effected by introducing a genetic modification preferably at the site of a gene encoding a CDC27 polypeptide having at least one inactive TPR domain in the NH2 terminal region of the polypeptide, preferably at that said genetic modification effected is site-directed mutagenesis or direct evolution. 43. A method for increasing the number of seeds in plants relative to suitable control plants, which comprises introducing and preferentially introducing into the bud apical meristem tissue of a plant a nucleic acid encoding a CDC27 polypeptide having at least one TPR domain. inactive in the NH2 terminal region of the polypeptide, and / or wherein said introduced nucleic acid is a splice variant or an allelic variant of a nucleic acid represented by SEQ ID NO: 129 or SEQ ID NO: 131, whose splice variant or variant allelic encodes a CDC27 polypeptide having at least one inactive TPR domain in the NH2 terminal region of the polypeptide, and / or wherein said introduced nucleic acid is capable of hybridizing to a nucleic acid as represented by SEQ ID NO: 129 or SEQ ID NO: 131 or with a binding variant or allelic variant as defined in claim 110, wherein said hybridizing sequence encodes a polypep CDC27 peptide having at least one inactive TPR domain in the NH2 terminal region of the polypeptide, and / or wherein said CDC27 nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the polypeptide NH2 terminal region encoding an ortholog or paralog of a polypeptide CDC27 represented by SEQ ID NO: 130 or SEQ ID NO: 132. Method according to any one of claims -41 to 43, characterized in that said CDC27 nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the NH2 terminal region of the polypeptide, or the polypeptide itself, is of origin vegetable, preferably from a dicotyledonous plant, preferably from the Brassicaceae family, still preferably from Arabidopsis thaliana. A method according to any one of claims -41 to 44, characterized in that said CDC27 nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the terminal NH2 region of the polypeptide is operably linked to an apical meristem promoter. preferably a bud early apical meristem promoter, preferably wherein said bud apical meristem promoter is an OSH1 promoter. Plant cell, characterized in that it is obtainable by a method as defined in any one of claims 41 to 45. 47. A plant cell transformed with a construct comprising: (i) a CDC27 nucleic acid encoding a CDC27 polypeptide having at least one inactive TPR domain in the NH2 terminal region of the polypeptide; and (ii) one or more control sequences capable of preferentially enhancing expression of the nucleic acid sequence of (i) in plant apical meristem tissue of a plant, preferably wherein said control sequence is an OSH1 promoter; and optionally (iii) a transcription termination sequence 48. A method of producing a transgenic plant having an increased seed number relative to appropriate control plants, comprising: (a) introducing and expressing into a plant a CDC27 nucleic acid encoding a CDC27 polypeptide having at least one TPR domain inactive at the NH2 terminal region of the polypeptide operably linked to a specific bud apical meristem promoter; and (b) cultivate the plant cell under conditions promoting plant growth and development. 49. A method for increasing seed production in a monocotyledonous plant over the production of suitable control plant seeds, characterized in that it preferably comprises increasing endosperm tissue expression of a monocotyledonous plant encoding a polypeptide domain comprising a domain. AT-hook is a DUF296 domain, preferably wherein said polypeptide further comprises one of the following reasons: Reason 1: QGQ V / I GG; or Reason 2: ILSLSGSFLPPPAPP; or Reason 3: NATYERLP; or Reason 4: SFTNVAYERLPL with none or an amino acid change at any position; or Reason 5: GRFEILSLTGSFLPGPAPPGSTGLTI YLAGGQGQVVGGSVVG with no, one or two amino acid changes at any position. A method according to claim 49, characterized in that said increased expression is effected by introducing a genetic modification preferably at the site of a gene encoding a polypeptide comprising an AT-hook domain and a DUF296 domain. 51. A method for increasing seed production in a monocotyledonous plant over the production of suitable control plant seeds, characterized in that it comprises introducing and expressing preferentially in endosperm tissue of a monocotyledonous plant of a nucleic acid encoding a polypeptide comprising a AT-hook domain and a DUF296 domain, and / or wherein said polypeptide further comprises one of the following reasons: Reason 1: QGQ V / 1 GG; or Reason 2: ILSLSGSFLPPPAPP; or Reason 3: NATYERLP; or Reason 4: SFTNVAYERLPL with none or an amino acid change at any position; or Reason 5: GRFEILSLTGSFLPGPAPPGSTGLTIY LAGGQGQVVGGSWG with no, one or two amino acid changes at any position. A method according to claim 51, characterized in that said nucleic acid is operably linked to an endosperm specific promoter, preferably to a prolamine promoter. A method according to any one of claims -127 to 133, characterized in that said nucleic acid is a portion or an allelic variant or a binding variant or a sequence capable of hybridizing to a sequence according to any of SEQ ID NO: -152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: -158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: -166, SEQ ID NO: 168 and SEQ ID NO: 170, wherein said portion, allelic variant, coupling variant, or hybridizing sequence encodes a polypeptide comprising an AT-hook domain and a DUF296 domain, and / or wherein said portion, allelic variant, splicing variant, or hybridizing sequence encodes an AT-hook protein ortholog or analog of SEQ ID NO: 153. A method according to any one of claims -49 to 53, characterized in that said nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain is of plant origin, preferably from a monocotyledonous plant, still preferably from Poaceae family, most preferably from the genus Oryza, most preferably from Oryza sativa. A method according to any one of claims -49 to 54, characterized in that said increased yield is selected from one or more of: increased total seed weight, increased full seed number, increased total seed number , increased number of flowers per panicle, increased harvest index (HI). Plant cell, characterized in that it is obtainable by a method as defined in any one of claims 49 to 55, wherein said plant or part thereof comprises a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain. whose nucleic acid is operably linked to an endosperm specific promoter. 57. A gene construct comprising: (a) a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain; (b) One or more control sequences capable of directing expression of the nucleic acid sequence of (i) in endosperm tissue of a monocotyledonous plant, preferably wherein said control sequence is a prolamine promoter; and optionally (c) a transcription termination sequence. Plant cell, characterized in that it is transformed with a construct as defined in claim 57. 59. A method for producing a monocotyledonous transgenic plant having increased seed production relative to appropriate control plants, characterized in that it comprises: (a) introducing and preferentially increasing endosperm tissue expression of a monocotyledonous plant of a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain; and (b) cultivate the plant cell under conditions promoting plant growth and development. 60. A method of increasing plant yield over suitable control plants, comprising increasing expression in a plant of a nucleic acid encoding a DOF (one-finger DNA binding) domain transcription factor polypeptide comprising ( i) as follows, and additionally or feature (ii) or (iii) as follows: (i) at least 60% sequence identity or with the DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and (ii) at least 70% sequence identity with the DOF domain represented by SEQ ID NO: 200; or (iii) Reason I: KALKKPDKILP (SEQ ID NO: 229) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or (iv) Reason II: DDPGIKLFGKTIPF (SEQ ID NO: 230) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position, and / or wherein said polypeptide comprising feature (i) and feature (iii) further comprises any, any two or all three of the (i) Reason III: SPTLGKHSRDE (SEQ ID NO: 231) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or (ii) Reason IV: LQANPAALSRSQNFQE (SEQ ID NO: 232) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or (iii) Reason V: KGEGCL WVPKTLRIDDPDEAAKSSIW TTLGIK (SEQ ID NO: 233) without any change; or with one or more conservative changes in any position; or with one, two, three, four or five non-conservative change (s) at any position, and / or wherein said polypeptide comprising feature (i) and feature (iii) comprises both Reason I and II. A method according to claim 60, characterized in that said increased expression is effected by introducing a genetic modification preferably at the site of a gene encoding a DOF transcription factor polypeptide, preferably wherein said genetic modification is effected by one of. : T-DNA activation, TILLING and homologous recombination. 62. A method of increasing plant yield over suitable control plants, comprising introducing and expressing into a plant a nucleic acid or variant thereof encoding a DOF transcription factor polypeptide comprising feature (i) as follows, and additionally or characteristic (ii) or (iii) as follows: (i) at least 60% sequence identity or with the DOF domain represented by SEQ ED NO: 200 or SEQ ID NO: 228; and (ii) at least 70% sequence identity with the DOF domain represented by SEQ ID NO: 200; or (iii) Reason I: KALKKPDKILP (SEQ ID NO: 229) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or (iv) Reason II: DDPGIKLFGKTIPF (SEQ ID NO: 230) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position, and / or wherein said polypeptide comprising feature (i) and feature (iii) further comprises any, any two or all three of the (i) Reason III: SPTLGKHSRDE (SEQ ID NO: 231) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or (ii) Reason IV: LQANPAALSRSQNFQE (SEQ ID NO: 232) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or (iii) Reason V: KGEGCLWVPKTLRIDDPDEAAKSSIWTTLIK (SEQ ID NO: 233) without any change; or with one or more conservative changes in any position; or with one, two, three, four or five non-conservative change (s) at any position, and / or wherein said polypeptide comprises feature (i) and feature (iii) comprises both Reason I and II. 63. The method of claim 62, wherein said nucleic acid or variant of the same DOF transcription factor is overexpressed in a plant. A method according to any one of claims -62 to 63, characterized in that said nucleic acid or variant thereof is of plant origin, preferably from a dicotyledonous plant, still preferably from the Brassicaceae family, more preferably the nucleic acid is of Arabidopsis thaliana. A method according to any one of claims -62 to 64, characterized in that said variant encodes a homologue of a DOF transcription factor protein of SEQ ID NO: 199 or SEQ ID NO: 227, preferably wherein homolog of a DOF transcription factor protein of SEQ ID NO: 199 is represented by any one of SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210 , SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220 and SEQ ID NO: 222, and / or wherein said homologue of a protein factor DOF transcription of SEQ ID NO: 227 is represented by any of SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 245, SEQ ID NO: 247, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253 and SEQ ID NO: 255. A method according to any one of claims -62 to 65, characterized in that said nucleic acid or variant thereof encoding a DOF transcription factor polypeptide is operably linked to a constitutive promoter, preferably wherein said constitutive promoter is. a GOS2 promoter, preferably a rice GOS2 promoter, and / or wherein said nucleic acid or variant thereof encoding a DOF transcription factor polypeptide is operably linked to a seed specific promoter, and / or wherein said seed specific promoter. The seed is an endosperm specific promoter, preferably a prolamine promoter, and / or wherein said constitutive promoter directs expression of a nucleic acid encoding a DOF transcription factor polypeptide comprising said characteristics (i) and (ii), and / or wherein said seed specific promoter directs expression of a nucleic acid encoding a polypeptide of DOF transcription comprising said features (i) and (iii). A method according to any one of claims 59 to 66, characterized in that said increased yield is selected from one or more of: increased number of full seeds, increased seed weight, increased number of flowers per panicle, increased seed fill rate, increased harvest index (HI), increased one thousand seed weight (TKW), increased root biomass, increased root length and increased root diameter. A method according to any one of claims 59 to 66, characterized in that said increased production occurs under mild dry stress conditions. Plant cell, characterized in that it is obtainable by a method as defined in any one of claims 59 to 68. 70. A composition comprising: (i) a nucleic acid or variant thereof encoding a DOF transcription factor polypeptide as defined in claim 62; (ii) one or more control sequences capable of directing expression of the nucleic acid sequence of (a) preferably wherein said control sequence is a constitutive promoter, more preferably wherein said control sequence is a seed specific promoter; and optionally (iii) a transcription termination sequence. 71. Plant cell, characterized in that it is transformed with a construct as defined in claim 70. 72. A method of producing a transgenic plant having an increased yield over the corresponding wild-type plant, comprising: (a) introducing and expressing in the plant, plant part or plant cell a nucleic acid or variant thereof encoding a DOF transcription factor polypeptide as defined in claim 62; (b) cultivate the plant cell under conditions promoting plant growth and development. 73. The method of claim 72, wherein said increased production occurs under mild dry stress conditions. 74. A method for increasing plant seed production over suitable control plants, which comprises preferentially reducing expression of an endogenous CKI gene in endosperm tissue of a plant, and / or preferentially reducing expression is effected by down-regulation. RNA-mediated gene expression, and / or wherein said RNA-mediated negative regulation is effected by co-suppression, and / or wherein said RNA-mediated negative regulation is effected by use of antisense CKI nucleic acid sequences and / or wherein said preferentially reducing expression is effected using an inverted repeat of a CKI gene or fragment thereof, preferably capable of forming a staple structure, and / or wherein said preferentially reducing expression is effected using ribozymes with specificity for one or more. CKI nucleic acid, and / or wherein said preferentially reducing expression is effected by mutagenesis by sermon. 75. The method of claim 74 wherein said preferentially reducing expression of an endogenous CKI gene in plant endosperm tissue is effected by an endosperm specific promoter, preferably a prolamine promoter. A method according to any one of claims -74 to 75, characterized in that said endogenous CKI gene is a CKI gene as found in a plant in its natural form or is an isolated CKI gene subsequently introduced into a plant. A method according to any one of claims -74 to 76, characterized in that said CKI gene / nucleic is from a plant source or artificial source, preferably that CKI nucleic acid sequences from monocotyledonous plants are used for transformation. of monocotyledonous plants, yet preferably that Poaceae CKI sequences are used for transformation into Poaceae family plants, more preferably that rice CKI nucleic acid sequences are used to transform rice plants. A method according to claim 77, characterized in that said rice CKI nucleic acid sequence comprises a sufficient length of substantially contiguous nucleotides of SEQ ID NO: 267 (OsCKI4) or comprises a sufficient length of substantially contiguous nucleotides. of a nucleic acid sequence encoding an OsCKI4 ortholog or paralog (SEQ ID NO: 267), and / or wherein said OsCKI4 ortholog or paralog is represented by SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO : 274, SEQ ID NO: 276, SEQ ID NO: 278 and SEQ ID NO: 280, and / or wherein said substantially contiguous nucleotides of a nucleic acid sequence encoding an OsCKI4 ortholog or paralog (SEQ ID NO: -267 ) are substantially contiguous nucleotides of nucleic acid sequences represented by SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: -273, SEQ ID NO: 275, SEQ ID NO: 277 and SEQ ID NO: 279. A method according to any one of claims -74 to 78, characterized in that said increased seed production is selected from one or more of the following: a) increased seed biomass; b) increased number of flowers per plant; c) increased number of seeds (full); and d) increased harvest index. Method according to any of claims -74 to 80, characterized in that said increased production occurs under mild stress conditions. Plant cell, characterized in that it is obtainable by a method according to any one of claims 74 to 80. 82. A method of producing a transgenic plant having increased seed production relative to appropriate control plants, comprising: (a) introducing and expressing in the plant, plant part or plant cell a gene construct comprising one or more more control sequences capable of preferentially directing expression of a CKI sense and / or antisense CKI nucleic acid sequence in plant endosperm tissue so as to silence an endogenous CKI gene in plant endosperm tissue; and (b) cultivate the plant, plant part or plant cell under conditions promoting plant growth and development. 83. <claim missing on the original document> 84. <claim missing on the original document> 85. <claim missing on the original document> 86. <claim missing on the original document> 87. <claim missing on the original document> 88. <claim missing on the original document> 89. <claim missing on the original document> 90. <claim missing on the original document> 91. <claim missing on the original document> 92. <claim missing on the original document> 93. <claim missing on the original document> 94. <claim missing on the original document> 95. <claim missing on the original document> 96. <claim missing on the original document> 97. <claim missing on the original document> 98. <claim missing on the original document> 99. <claim missing on the original document> 100. <claim missing on the original document> 101. <claim missing on the original document> 102. <claim missing on the original document> 103. <claim missing on the original document> 104. <claim missing on the original document> 105. <claim missing on the original document> 106. <claim missing on the original document> 107. <claim missing on the original document> 108. <claim missing on the original document> 109. <claim missing on the original document> 110. <claim missing on the original document> 111. <claim missing on the original document> 112. <claim missing on the original document> 113. <claim missing on the original document> 114. <claim missing on the original document> 115. <claim missing on the original document> 116. <claim missing on the original document> 117. <claim missing on the original document> 118. <claim missing on the original document> 119. <claim missing on the original document> 120. <claim missing on the original document> 121. <claim missing on the original document> 122. <claim missing on the original document> 123. <claim missing on the original document> 124. <claim missing on the original document> 125. <claim missing on the original document> 126. <claim missing on the original document> 127. <claim missing on the original document> 128. <claim missing on the original document> 129. <claim missing on the original document> 130. <claim missing on the original document> 131. <claim missing on the original document> 132. <claim missing on the original document> 133. <claim missing on the original document> 134. <claim missing on the original document> 135. <claim missing on the original document> A method according to any one of claims -127 to 135, characterized in that said nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain is of plant origin, preferably from a monocotyledonous plant, still preferably from Poaceae family, most preferably from the genus Oiyza, most preferably from Oryza sativa. 137. A method according to any one of claims -127 to 136, characterized in that said increased yield is selected from one or more of: increased total seed weight, increased full seed number, increased total seed number. , increased number of flowers per panicle, increased harvest index (HI). Plant or part thereof including plant cell obtainable by a method as defined in any one of claims -127 to 137, characterized in that said plant or part thereof comprises a nucleic acid encoding a polypeptide comprising an AT-hook domain. and a DUF296 domain whose nucleic acid is operably linked to an endosperm specific promoter. 139. A gene construct comprising: (a) A nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain; (b) One or more control sequences capable of directing expression of the nucleic acid sequence of (i) in endosperm tissue of a monocotyledonous plant; and optionally (c) a transcription termination sequence. Use of a construct as defined in claim -139, characterized in that it is for increasing seed production in monocotyledonous plants. 141. Construction according to claim 139, characterized in that said control sequence is a prolamine promoter. 142. Plant, plant part or plant cell, characterized in that it is transformed with a construction as defined in claim 139. 143. A method for producing a monocotyledonous transgenic plant having increased seed yield over appropriate control plants, characterized in that it comprises: (a) introducing and preferentially increasing endosperm tissue expression of a monocotyledonous plant of a nucleic acid encoding a polypeptide comprising an AT-hook domain and a DUF296 domain; and (b) cultivate the plant cell under conditions promoting plant growth and development. 144. Transgenic monocotyledonous plant, characterized in that it has increased seed yield over appropriate control plants, said increased seed yield resulting from preferential expression in endosperm tissue of a monocotyledonous plant encoding a polypeptide domain comprising a domain. AT-hook is a DUF296 domain. A monocotyledonous transgenic plant according to claim 138, 142 or 144, characterized in that said plant is a cereal, such as rice, corn, sugar cane, wheat, barley, millet, rye, sorghum, grasses. or oatmeal. 146. Harvestable parts of a plant as defined in any one of claims 138, 142, 144 or 145, characterized in that said harvestable parts are seeds. 147. Products characterized in that they are derived from a plant as defined in claim 145 and / or parts which may be harvested from a plant as defined in claim 146. 148. Use of a polypeptide-encoding nucleic acid comprising an AT-hook domain and a DUF296 domain, whose nucleic acid is operably linked to an endosperm-specific promoter, characterized in that it is for increasing seed production in a monocotyledonous plant compared with seed production in a suitable control plant. Use according to claim 148, characterized in that said increased seed yield is selected from one or more of: increased total seed weight, increased full seed, increased total seed number flowers per panicle, increased harvest index (HI). 150. A method of increasing plant yield over suitable control plants, comprising increasing expression in a plant of a nucleic acid encoding a DOF (one-finger DNA binding) domain transcription factor polypeptide comprising ( i) as follows, and additionally or feature (ii) or (iii) as follows: (i) at least 60% sequence identity or with the DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and (ii) at least 70% sequence identity with the DOF domain represented by SEQ ID NO: 200; or (iii) Reason I: KALKKPDKILP (SEQ ID NO: 229) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or Reason II: DDPGIKLFGKTIPF (SEQ ID NO: 230) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position. 151. The method of claim 150, wherein said polypeptide comprising feature (i) and feature (iii) further comprises any one, any two or all of the following reasons: - Reason III: SPTLGKHSRDE (SEQ ID NO: 231) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or - Reason IV: LQANPAALSRSQNFQE (SEQ ID NO: 232) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or - Reason V: KGEGCL WVPKTLRIDDPDEAAKSSIW TTLGIK (SEQ ID NO: 233) without any change; or with one or more conservative changes in any position; or with one, two, three, four or five non-conservative change (s) in any position. Method according to claim 150 or 151, characterized in that said polypeptide comprising feature (i) and feature (iii) comprises both Reason I and II. A method according to any one of claims 150 to 152, characterized in that said increased expression is effected by introducing a genetic modification preferably at the site of a gene encoding a DOF transcription factor polypeptide. 154. The method of claim 153 wherein said genetic modification is effected by one of: T-DNA activation, TILLING and homologous recombination. 155. A method of increasing plant yield over suitable control plants, comprising introducing and expressing into a plant a nucleic acid or variant thereof encoding a DOF transcription factor polypeptide comprising feature (i) as follows, and additionally or characteristic (ii) or (iii) as follows: (i) at least 60% sequence identity or with the DOF domain represented by SEQ ID NO: 200 or SEQ ID NO: 228; and (ii) at least 70% sequence identity with the DOF domain represented by SEQ ID NO: 200; or (iii) Reason I: KALKKPDKILP (SEQ ID NO: 229) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or Reason II: DDPGUCLFGKTIPF (SEQ ID NO: 230) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position. 156. The method of claim 155, wherein said polypeptide comprising feature (i) and feature (iii) further comprises any one, any two or all three of the following reasons: Reason III: SPTLGKHSRDE (SEQ ID NO: 231) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or - Reason IV: LQANPAALSRSQNFQE (SEQ ID NO: 232) without any change; or with one or more conservative changes in any position; or with one, two or three non-conservative change (s) at any position; and / or - Reason V: KGEGCLWVPKTLRIDDPDEAAKSSIWTTLIK (SEQ ID NO: 233) without any change; or with one or more conservative changes in any position; or with one, two, three, four or five non-conservative change (s) in any position. 157. The method of claim 155 or 156, wherein said polypeptide comprises feature (i) and feature (iii) comprises both Reason I and II. Method according to claims 155 to 157, characterized in that said nucleic acid or variant of the same transcription factor DOF is overexpressed in a plant. A method according to any one of claims -155 to 158, characterized in that said nucleic acid or variant thereof is of plant origin, preferably from a dicotyledonous plant, still preferably from the Brassicaceae family, more preferably the nucleic acid is of Arabidopsis thaliana. Method according to any of claims -155 to 159, characterized in that said variant encodes a homologue of a DOF transcription factor protein of SEQ ID NO: 199 or SEQ ID NO: 227. 161. The method of claim 160, wherein said homolog of a DOF transcription factor protein of SEQ ID NO: 199 is represented by any one of SEQ ID NO: 202, SEQ ID NO: 204, SEQ SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220 and SEQ ID NO : 222. Method according to claim 160, characterized in that said homolog of a DOF transcription factor protein of SEQ ID NO: 227 is represented by any one of SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 245, SEQ ID NO: 247, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253 and SEQ ID NO : 255. A method according to any one of claims -155 to 162, characterized in that said nucleic acid or variant thereof encoding a DOF transcription factor polypeptide is operably linked to a constitutive promoter. 164. The method of claim 163, wherein said constitutive promoter is a GOS2 promoter, preferably a rice GOS2 promoter. A method according to any one of claims -155 to 162, characterized in that said nucleic acid or variant thereof encoding a DOF transcription factor polypeptide is operably linked to a seed specific promoter. 166. The method of claim 165, wherein said seed specific promoter is an endosperm specific promoter, preferably a prolamine promoter. 167. The method of claim 163 or 164, wherein said constitutive promoter directs expression of a nucleic acid encoding a DOF transcription factor polypeptide comprising said characteristics (i) and (ii). 168. The method of claim 165 or 166, wherein said seed-specific promoter directs expression of a nucleic acid encoding a DOF transcription factor polypeptide comprising said characteristics (i) and (iii). A method according to any one of claims 150 to 168, characterized in that said increased yield is selected from one or more of: increased number of full seeds, increased seed weight, increased number of flowers per panicle. , increased seed filling rate, increased harvest index (HI), increased one thousand seed weight (TKW) 5 increased root biomass, increased root length and increased root diameter. 170. A method according to any one of claims -150 to 169, characterized in that said increased production occurs under mild dry stress conditions. 171. Plant or part thereof, characterized in that it is obtainable by a method as defined in any one of claims -150 to 170. Construction, characterized in that it comprises: (i) a nucleic acid or variant thereof encoding a DOF transcription factor polypeptide as defined in claim 155; (ii) one or more control sequences capable of directing expression of the nucleic acid sequence of (a), and optionally (iii) a transcription termination sequence. 173. Construction according to claim 172, characterized in that said control sequence is a constitutive promoter. 174. Construction according to claim 172, characterized in that said control sequence is a seed specific promoter. 175. Plant, plant part or plant cell, characterized in that it is transformed with a construction as defined in any one of claims 172 to 174. 176. A method for producing a transgenic plant having increased yield over the corresponding wild-type plant, characterized in that it comprises: (a) introducing and expressing into the plant, plant part or plant cell a nucleic acid or variant thereof encoding a DOF transcription factor polypeptide as defined in claim 155; (b) cultivate the plant cell under conditions promoting plant growth and development. 177. The method of claim 176 wherein said increased production occurs under mild dry stress conditions. 178. Transgenic plant, characterized in that it has increased yield over the corresponding wild-type plant, said increased yield resulting from a nucleic acid or variant thereof encoding a DOF transcription factor polypeptide as defined in claim 155 introduced into said plant . Transgenic plant according to claim 171, -175 or 178, characterized in that said plant is a monocotyledonous plant such as sugar cane or that the plant is a cereal such as rice, corn, wheat , barley, millet, rye, oats or sorghum. Harvestable parts of a plant, characterized in that they are according to any one of claims 171, -175, 178 or 179. 181. Harvestable parts of a plant as defined in claim 180, characterized in that said harvestable parts are seeds. Products, characterized in that they are derived from a plant as defined in claim 179 and / or parts that can be harvested from a plant as defined in claims 180 or 181. 183. Use of a nucleic acid or variant thereof encoding a polypeptide, or use of such a polypeptide, the polypeptide being of DOF transcription factor, characterized in that it is for increasing plant yield over appropriate control plants. Use according to claim 183, characterized in that said increased yield is selected from one or more of: increased number of full seeds, increased seed weight, increased number of flowers per panicle, fill rate of increased seeds, increased harvest index (HI), increased one thousand seed weight (TKW), increased root biomass, increased root length and increased root diameter. Use according to claim 183 or 184, characterized in that said increased production occurs under mild dry stress conditions. 186. Use of a nucleic acid or variant thereof encoding a polypeptide, or use of such a polypeptide, the polypeptide being a DOF transcription factor polypeptide, characterized in that it is a molecular marker. 187. A method for increasing plant seed yield over suitable control plants, which comprises preferentially reducing expression of an endogenous CKJ gene in endosperm tissue of a plant. 188. The method of claim 187, wherein said preferentially reducing expression is effected by RNA-mediated down-regulation of gene expression. 189. The method of claim 188, characterized in that said RNA-mediated down-regulation is effected by co-suppression. 190. The method of claim 188, wherein said RNA-mediated down-regulation is effected by use of antisense CKI nucleic acid sequences. 191. The method of claim 187, wherein said preferentially reducing expression is effected using an inverted repeat of a CKI gene or fragment thereof, preferably capable of forming a staple structure. 192. The method of claim 187, wherein said preferentially reducing expression is effected using ribozymes with specificity for a CKL nucleic acid. Method according to claim 187, characterized in that said preferentially reducing expression is effected by insertion mutagenesis. A method according to any one of claims -187 to 193, wherein said preferentially reducing expression of an endogenous CKI gene in plant endosperm tissue is effected by an endosperm-specific promoter, preferably a prolamine promoter. . A method according to any one of claims -187 to 194, characterized in that said endogenous CKI gene is a CKI gene as found in a plant in its natural form or is an isolated CKI gene subsequently introduced into a plant. A method according to any one of claims -187 to 197, characterized in that said CKI gene / nucleic is from a plant source or artificial source, preferably that CKI nucleic acid sequences from monocotyledonous plants are used for transformation. of monocotyledonous plants, yet preferably that Poaceae CKI sequences are used for transformation into Poaceae family plants, more preferably that rice CKI nucleic acid sequences are used to transform rice plants. A method according to claim 196, characterized in that said rice CKI nucleic acid sequence comprises a sufficient length of substantially contiguous nucleotides of SEQ ID NO: 267 (OsCKI4) or comprises a sufficient length of substantially contiguous nucleotides. of a nucleic acid sequence encoding an OsCKI4 ortholog or paralog (SEQ ID NO: 267). A method according to claim 197, characterized in that said OsCKI4 orthologs or paralogs are represented by SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 274, SEQ ID NO: 276, SEQ ID NO: 278 and SEQ ID NO: 280. 199. The method according to claim 197 or 198, wherein said substantially contiguous nucleotides of a nucleic acid sequence encoding an OsCKI4 ortholog or paralog (SEQ ID NO: 267) are substantially contiguous nucleotides of nucleic acid sequences. represented by SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 273, SEQ ID NO: 275, SEQ ID NO: 277 and SEQ ID NO: 279. Method according to any one of claims -187 to 199, characterized in that said increased seed production is selected from one or more of the following: a) increased seed biomass; b) increased number of flowers per plant; c) increased number of seeds (full); and d) increased harvest index. Method according to any one of claims -187 to 200, characterized in that said increased production occurs under mild stress conditions. Plant or part thereof, characterized in that it is obtainable by a method according to any one of claims 187 to 201. 203. A method of producing a transgenic plant having increased seed production relative to appropriate control plants, comprising: (a) introducing and expressing in the plant, plant part or plant cell a gene construct comprising one or more more control sequences capable of preferentially directing expression of a CKI sense and / or antisense CKI nucleic acid sequence in plant endosperm tissue so as to silence an endogenous CKI gene in plant endosperm tissue; and (b) cultivate the plant, plant part or plant cell under conditions promoting plant growth and development. 204. Use of CKI nucleic acid, characterized in that it is for the reduction or substantial elimination of endogenous CKI gene expression in plant endosperm tissue to increase plant seed production relative to appropriate control plants. Use according to claim 204, characterized in that said increased production is selected from one or more of: selected from one or more of the following: a) increased seed biomass; b) increased number of flowers per plant; c) increased number of seeds (full); and d) increased harvest index. 206. Use according to claim 204 or 205, characterized in that said seed production occurs under mild stress conditions.