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BRPI0605166A - método de determinação de ácido metilmalÈnico baseado na extração alquilativa associada à cromatografia lìquida acoplada à espectrometria de massas - Google Patents

método de determinação de ácido metilmalÈnico baseado na extração alquilativa associada à cromatografia lìquida acoplada à espectrometria de massas

Info

Publication number
BRPI0605166A
BRPI0605166A BRPI0605166-9A BRPI0605166A BRPI0605166A BR PI0605166 A BRPI0605166 A BR PI0605166A BR PI0605166 A BRPI0605166 A BR PI0605166A BR PI0605166 A BRPI0605166 A BR PI0605166A
Authority
BR
Brazil
Prior art keywords
methylmalonic acid
mass spectrometry
alkylative
chromatography coupled
method based
Prior art date
Application number
BRPI0605166-9A
Other languages
English (en)
Inventor
Valdemir Melechco Carvalho
Fernando Kok
Original Assignee
Inst Fleury
Valdemir Melechco Carvalho
Fernando Kok
Fleury S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Fleury, Valdemir Melechco Carvalho, Fernando Kok, Fleury S A filed Critical Inst Fleury
Priority to BRPI0605166-9A priority Critical patent/BRPI0605166A/pt
Priority to US12/312,250 priority patent/US8067242B2/en
Priority to PCT/BR2007/000302 priority patent/WO2008052299A2/en
Publication of BRPI0605166A publication Critical patent/BRPI0605166A/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/117497—Automated chemical analysis with a continuously flowing sample or carrier stream
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/20—Oxygen containing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/20—Oxygen containing
    • Y10T436/200833—Carbonyl, ether, aldehyde or ketone containing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/20—Oxygen containing
    • Y10T436/200833—Carbonyl, ether, aldehyde or ketone containing
    • Y10T436/201666—Carboxylic acid
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

MéTODO DE DETERMINAçãO DE áCIDO METILMALÈNICO BASEADO NA EXTRAçãO ALQUILATIVA ASSOCIADA à CROMATOGRAF1A LìQUIDA ACOPLADA à ESPECTROMETRIA DE MASSAS. Refere-se a presente invenção a um método para a determinação da presença de ácido metilmalónico em amostras biológicas compreendendo as etapas de extração do ácido metilmalónico da amostra; derivatização do ácido metilmalónico e; uso da espectrometria de massas e ionização a pressão atmosférica no modo negativo para determinar a presença do ácido metilmalónico. Um objetivo adicional da presente invenção diz respeito a kits diagnósticos para a determinação da presença e quantificação de ácido metilmalónico baseados no método antes mencionado.
BRPI0605166-9A 2006-11-03 2006-11-03 método de determinação de ácido metilmalÈnico baseado na extração alquilativa associada à cromatografia lìquida acoplada à espectrometria de massas BRPI0605166A (pt)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BRPI0605166-9A BRPI0605166A (pt) 2006-11-03 2006-11-03 método de determinação de ácido metilmalÈnico baseado na extração alquilativa associada à cromatografia lìquida acoplada à espectrometria de massas
US12/312,250 US8067242B2 (en) 2006-11-03 2007-11-05 Method for methylmalonic acid detemination based on alkylative extraction associated to liquid chromatography coupled to mass spectrometry
PCT/BR2007/000302 WO2008052299A2 (en) 2006-11-03 2007-11-05 Method for methylmalonic acid determination based on alkylative extraction associated to liquid chromatography coupled to mass spectrometry

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BRPI0605166-9A BRPI0605166A (pt) 2006-11-03 2006-11-03 método de determinação de ácido metilmalÈnico baseado na extração alquilativa associada à cromatografia lìquida acoplada à espectrometria de massas

Publications (1)

Publication Number Publication Date
BRPI0605166A true BRPI0605166A (pt) 2008-07-01

Family

ID=39344616

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0605166-9A BRPI0605166A (pt) 2006-11-03 2006-11-03 método de determinação de ácido metilmalÈnico baseado na extração alquilativa associada à cromatografia lìquida acoplada à espectrometria de massas

Country Status (3)

Country Link
US (1) US8067242B2 (pt)
BR (1) BRPI0605166A (pt)
WO (1) WO2008052299A2 (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10837972B2 (en) 2010-06-09 2020-11-17 Quest Diagnostics Investments Incorporated Mass spectrometric determination of derivatized methylmalonic acid
CN102539595B (zh) * 2010-12-09 2014-09-03 北京国立柏林医学科技发展有限公司 检测血液或尿液中甲基丙二酸含量的方法
CN102621248B (zh) * 2012-04-18 2013-12-18 长沙高新开发区鷁巢生物科技有限公司 一种一步衍生法同步分析碱基、核苷、有机酸、脂肪酸、氨基酸及糖类代谢产物的方法
US9732296B2 (en) 2015-03-20 2017-08-15 Authentix, Inc. Fuel markers and methods of producing and using same
CN111638287A (zh) * 2020-06-11 2020-09-08 成都益康谱科技有限公司 一种检测人体干尿滤纸片中多种有机酸的定量检测方法
CN112834682B (zh) * 2021-01-05 2023-09-22 北京和合医学诊断技术股份有限公司 检测血液中甲基丙二酸含量的方法
CN113917018B (zh) * 2021-09-30 2023-07-25 上海中科新生命生物科技有限公司 一种同时检测水溶性维生素及代谢产物的沉淀剂和方法
CN114994194B (zh) * 2022-05-09 2024-04-09 广东安纳检测技术有限公司 一种水质中丙二酸的测定方法及装置
CN115144499A (zh) * 2022-07-04 2022-10-04 华润化学材料科技股份有限公司 一种甄别可降解塑料及其制品的方法与应用
CN115508483A (zh) * 2022-09-22 2022-12-23 武汉迈特维尔生物科技有限公司 一种快速检测血清样本中甲基丙二酸的lc-ms/ms方法
CN116818966A (zh) * 2023-06-29 2023-09-29 山东英盛生物技术有限公司 Lc-ms/ms测定甲基丙二酸、同型半胱氨酸及其相关代谢产物方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4990458A (en) * 1982-04-13 1991-02-05 Mcmaster University Derivatization of organic compounds during their analysis or determination
US5457055A (en) * 1986-11-20 1995-10-10 The University Of Colorado Foundation Diagnostic method for cobalamin deficiency
US5585070A (en) * 1994-04-29 1996-12-17 Phoenix International Life Sciences Inc. Method for extraction, extraction cartridge and automated extraction processing system
US5508204A (en) * 1995-01-12 1996-04-16 Norman Clinical Laboratories, Inc. Multiple sample sequential chemical analysis
MXPA02004153A (es) * 1999-10-27 2002-10-17 Kosan Biosciences Inc Produccion heterologa de policetidos.
CA2343985C (en) * 2000-04-17 2011-01-11 Mark Kushnir Method of analyzing dicarboxylic acids
US7158903B2 (en) * 2002-03-15 2007-01-02 University Of Utah Methods for quantitative analysis by tandem mass spectrometry
US20050164402A1 (en) * 2003-07-14 2005-07-28 Belisle Christopher M. Sample presentation device
GB0423755D0 (en) * 2004-10-26 2004-11-24 Gene Bridges Gmbh Methods for heterologus expression of secondary metabolites
JP4506538B2 (ja) * 2005-03-31 2010-07-21 株式会社島津製作所 質量分析方法

Also Published As

Publication number Publication date
WO2008052299B1 (en) 2008-08-07
WO2008052299A3 (en) 2008-06-19
US8067242B2 (en) 2011-11-29
US20100304492A1 (en) 2010-12-02
WO2008052299A2 (en) 2008-05-08

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Legal Events

Date Code Title Description
B03A Publication of a patent application or of a certificate of addition of invention [chapter 3.1 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B15K Others concerning applications: alteration of classification

Ipc: G01N 33/50 (2006.01), G01N 33/82 (2006.01)

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements