[go: up one dir, main page]

BRPI0509179A - método de crescimento de célula tronco pluripotente - Google Patents

método de crescimento de célula tronco pluripotente

Info

Publication number
BRPI0509179A
BRPI0509179A BRPI0509179-9A BRPI0509179A BRPI0509179A BR PI0509179 A BRPI0509179 A BR PI0509179A BR PI0509179 A BRPI0509179 A BR PI0509179A BR PI0509179 A BRPI0509179 A BR PI0509179A
Authority
BR
Brazil
Prior art keywords
pluripotent stem
cells
growth method
cell growth
stem cell
Prior art date
Application number
BRPI0509179-9A
Other languages
English (en)
Inventor
Toshihiro Akaike
Keiichi Fukuda
Masato Nagaoka
Uichi Koshimizu
Original Assignee
Daiichi Asubio Pharma Co Ltd
Toshihiro Akaike
Keiichi Fukuda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daiichi Asubio Pharma Co Ltd, Toshihiro Akaike, Keiichi Fukuda filed Critical Daiichi Asubio Pharma Co Ltd
Publication of BRPI0509179A publication Critical patent/BRPI0509179A/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MéTODO DE CRESCIMENTO DE CéLULA TRONCO PLURIPOTENTE. Um novo método de crescimento é fornecido para células tronco pluripotentes, tais como células ES. O método da invenção é um método de crescimento de células tronco pluripotentes e um método de transferência de genes no qual as células tronco pluripotentes são cultivadas sob condições que mantêm o seu estado não-diferenciado e a sua pluripotência, o método sendo caracterizado pelo uso de um meio líquido e de um recipiente de cultivo com uma molécula imobilizada ou revestida na superfície de um substrato de fase sólida a qual é adesiva às células tronco pluripotentes em uma concentração fixa, para crescer as células pluripotentes em um estado disperso enquanto mantendo seu estado não-diferenciado e pluripotência, sem usar células alimentadoras ou para transferir e expressar um gene nesse ponto.
BRPI0509179-9A 2004-03-23 2005-03-23 método de crescimento de célula tronco pluripotente BRPI0509179A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004085393 2004-03-23
PCT/JP2005/006006 WO2005090557A1 (ja) 2004-03-23 2005-03-23 多能性幹細胞の増殖方法

Publications (1)

Publication Number Publication Date
BRPI0509179A true BRPI0509179A (pt) 2007-09-18

Family

ID=34993698

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0509179-9A BRPI0509179A (pt) 2004-03-23 2005-03-23 método de crescimento de célula tronco pluripotente

Country Status (12)

Country Link
US (1) US7892835B2 (pt)
EP (1) EP1734112B1 (pt)
JP (1) JP4688793B2 (pt)
KR (1) KR101178786B1 (pt)
CN (1) CN1934245B (pt)
AU (1) AU2005224569B2 (pt)
BR (1) BRPI0509179A (pt)
CA (1) CA2560581C (pt)
IL (1) IL178232A (pt)
RU (1) RU2375448C2 (pt)
SG (1) SG150567A1 (pt)
WO (1) WO2005090557A1 (pt)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1572984E (pt) 2002-12-16 2016-06-03 Technion Res & Dev Foundation Sistema de cultura para células estaminais embrionárias humanas sem células alimentadoras e sem xeno
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US8476070B2 (en) 2005-08-29 2013-07-02 Technion Research & Development Foundation Limited Media for culturing stem cells
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
ZA200804673B (en) 2005-12-13 2009-11-25 Univ Kyoto Nuclear reprogramming factor
US8129187B2 (en) * 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
GB0602063D0 (en) 2006-02-02 2006-03-15 Univ Manchester Cell Culture
EP2733203B1 (en) 2006-08-02 2018-10-10 Technion Research & Development Foundation Ltd. Methods of expanding embryonic stem cells in a suspension culture
WO2008087257A1 (en) * 2007-01-18 2008-07-24 Suomen Punainen Risti, Veripalvelu Novel methods and reagents directed to production of cells
CA2683056C (en) 2007-04-07 2020-03-24 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
WO2008137115A1 (en) 2007-05-03 2008-11-13 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
US8574567B2 (en) 2007-05-03 2013-11-05 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
EP2610336A1 (en) 2007-07-31 2013-07-03 Lifescan, Inc. Differentiation of human embryonic stem cells
US9683232B2 (en) * 2007-12-10 2017-06-20 Kyoto University Efficient method for nuclear reprogramming
KR101532442B1 (ko) * 2007-12-10 2015-06-29 고쿠리츠 다이가쿠 호진 교토 다이가쿠 효율적인 핵 초기화 방법
MX2010009251A (es) 2008-02-21 2010-11-25 Centocor Ortho Biotech Inc Metodos, placas de superficie modificada y composiciones para la fijacion, el cultivo y el desprendimiento celular.
US20100279404A1 (en) 2008-05-02 2010-11-04 Shinya Yamanaka Method of nuclear reprogramming
CA3060170A1 (en) 2008-06-13 2009-12-17 Whitehead Institute For Biomedical Research Programming and reprogramming of cells
ES2697798T3 (es) 2008-06-30 2019-01-28 Janssen Biotech Inc Diferenciación de células madre pluripotentes
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
CA2744227C (en) 2008-11-20 2018-10-02 Centocor Ortho Biotech Inc. Methods and compositions for cell attachment and cultivation on planar substrates
JP2012509085A (ja) 2008-11-20 2012-04-19 ヤンセン バイオテツク,インコーポレーテツド マイクロキャリア上での多能性幹細胞の培養
EP2456862A4 (en) 2009-07-20 2013-02-27 Janssen Biotech Inc DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
ES2779048T3 (es) 2009-11-12 2020-08-13 Technion Res & Dev Foundation Medios de cultivo, cultivos celulares y métodos de cultivo de células madre pluripotentes en un estado indiferenciado
DK2516625T3 (da) 2009-12-23 2024-09-09 Janssen Biotech Inc Differentiering af humane embryonale stamceller
AU2011223900A1 (en) 2010-03-01 2012-09-13 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
US9567378B2 (en) * 2010-06-03 2017-02-14 New York University In situ oriented immobilization of proteins on a support
AU2013204021B2 (en) * 2010-06-11 2015-07-30 Regeneron Pharmaceuticals, Inc. Production of fertile xy female animals from xy es cells
CA2800534C (en) 2010-06-11 2019-01-22 Regeneron Pharmaceuticals, Inc. Production of fertile xy female animals from xy es cells
SG187946A1 (en) 2010-08-31 2013-03-28 Janssen Biotech Inc Differentiation of human embryonic stem cells
SG10201509953VA (en) * 2010-09-07 2016-01-28 Technion Res & Dev Foundation Novel Methods And Culture Media For Culturing Pluripotent Stem Cells
US9951309B2 (en) * 2011-02-18 2018-04-24 Stemcyte Inc. Stem cell packaging and shipping
AU2012239183B2 (en) * 2011-04-08 2016-08-25 Otsuka Pharmaceutical Factory, Inc. Method for producing sheet-like pancreatic islet
JP5862061B2 (ja) * 2011-06-10 2016-02-16 Dic株式会社 胚性幹細胞の培養方法
RU2517112C2 (ru) * 2011-07-08 2014-05-27 Общество с ограниченной ответственностью г. Санкт-Петербург "Покровский банк стволовых клеток" Способ культивирования мезенхимных стволовых клеток, выделенных из костного мозга
RU2495124C2 (ru) * 2011-12-07 2013-10-10 Федеральное государственное бюджетное учреждение "Новосибирский научно-исследовательский институт патологии кровообращения имени академика Е.Н. Мешалкина" Министерства здравоохранения Российской Федерации (ФГБУ "ННИИПК им. акад. Е.Н. Мешалкина" Минздрава России) РЕКОМБИНАНТНАЯ ПЛАЗМИДА pSN-ZsGreen, КОДИРУЮЩАЯ БЕЛКИ SOX2 И NANOG ЧЕЛОВЕКА И ФЛУОРЕСЦЕНТНЫЙ БЕЛОК ZsGreen, ПРЕДНАЗНАЧЕННАЯ ДЛЯ ПОЛУЧЕНИЯ ИНДУЦИРОВАННЫХ ПЛЮРИПОТЕНТНЫХ СТВОЛОВЫХ КЛЕТОК ЧЕЛОВЕКА
KR102090751B1 (ko) 2011-12-22 2020-03-19 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 단일 인슐린 호르몬 양성 세포로의 분화
CN108034633B (zh) 2012-06-08 2022-08-02 詹森生物科技公司 人胚胎干细胞向胰腺内分泌细胞的分化
US9175263B2 (en) * 2012-08-22 2015-11-03 Biotime, Inc. Methods and compositions for targeting progenitor cell lines
JP2014082956A (ja) * 2012-10-19 2014-05-12 Somar Corp 細胞培養基材、およびそれを用いた細胞培養方法並びに多能性幹細胞の分化誘導方法
MX2015008578A (es) 2012-12-31 2015-09-07 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas en celulas endocrinas pancreaticas mediante el uso de relugadores de hb9.
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
CN105705634A (zh) 2012-12-31 2016-06-22 詹森生物科技公司 用于分化成胰腺内分泌细胞的人多能细胞的悬浮和群集
RU2018116647A (ru) 2012-12-31 2018-10-24 Янссен Байотек, Инк. Культивация эмбриональных стволовых клеток человека в воздушно-жидкостной зоне взаимодействия с целью их дифференцировки в панкреатические эндокринные клетки
JP6054223B2 (ja) * 2013-03-22 2016-12-27 株式会社Ihi 幹細胞培養装置、幹細胞培養方法
JP6529486B2 (ja) 2013-06-05 2019-06-12 バイオタイム インク.Biotime Inc. 哺乳動物種における誘導組織再生のための組成物および方法
JP2014036660A (ja) * 2013-10-09 2014-02-27 Viacyte Inc 幹細胞集合体懸濁液組成物、その分化方法
US11078462B2 (en) 2014-02-18 2021-08-03 ReCyte Therapeutics, Inc. Perivascular stromal cells from primate pluripotent stem cells
CN117821369A (zh) 2014-05-16 2024-04-05 詹森生物科技公司 小分子增强胰腺内分泌细胞中的mafa表达的用途
WO2015200805A2 (en) 2014-06-26 2015-12-30 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modifications and methods of use
US10240127B2 (en) 2014-07-03 2019-03-26 ReCyte Therapeutics, Inc. Exosomes from clonal progenitor cells
PT3207124T (pt) 2014-10-15 2019-08-27 Regeneron Pharma Métodos e composições para a produção ou conservação de células pluripotentes
EP3234109A4 (en) 2014-12-19 2018-06-27 Janssen Biotech, Inc. Suspension culturing of pluripotent stem cells
US11066641B2 (en) 2015-01-15 2021-07-20 Osaka University Method for inducing differentiation of corneal epithelial cells from pluripotent stem cells
CN107208064A (zh) * 2015-01-29 2017-09-26 国立大学法人东京大学 细胞的培养方法、细胞的凝集体、细胞凝集控制剂以及培养基
CN104651300B (zh) * 2015-02-11 2018-10-12 南开大学 一种三维复合细胞聚集体模型及其制备方法与应用
CA2990476C (en) 2015-07-10 2021-09-07 Heartseed Inc. Method for producing high-quality ips cells
CN108697068B (zh) 2015-09-17 2022-02-11 瑞泽恩制药公司 对用于产生能生育的xy雌性小鼠的多能细胞的选择
WO2017100313A1 (en) 2015-12-07 2017-06-15 Biotime, Inc. Methods for the re-derivation of diverse pluripotent stem cell-derive brown fat cells
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
US11306287B1 (en) * 2016-06-16 2022-04-19 Regents Of The University Of Minnesota Method of generating skeletal muscle stem cells from pluripotent cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6667176B1 (en) * 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
JP2001061470A (ja) * 1999-08-30 2001-03-13 Science & Tech Agency マウス胚性幹細胞
US7005252B1 (en) 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US20030190704A1 (en) 2002-04-04 2003-10-09 Ting Xie Methods and compositions for anchoring stem cells in a microenvironment

Also Published As

Publication number Publication date
CN1934245A (zh) 2007-03-21
EP1734112B1 (en) 2017-08-23
JPWO2005090557A1 (ja) 2008-02-07
JP4688793B2 (ja) 2011-05-25
SG150567A1 (en) 2009-03-30
CA2560581A1 (en) 2005-09-29
AU2005224569A1 (en) 2005-09-29
CN1934245B (zh) 2012-07-04
US7892835B2 (en) 2011-02-22
KR20060129486A (ko) 2006-12-15
EP1734112A4 (en) 2007-09-05
IL178232A0 (en) 2006-12-31
US20070155013A1 (en) 2007-07-05
CA2560581C (en) 2013-06-11
IL178232A (en) 2012-01-31
EP1734112A1 (en) 2006-12-20
AU2005224569B2 (en) 2011-07-14
KR101178786B1 (ko) 2012-09-07
WO2005090557A1 (ja) 2005-09-29
RU2006137300A (ru) 2008-04-27
RU2375448C2 (ru) 2009-12-10

Similar Documents

Publication Publication Date Title
BRPI0509179A (pt) método de crescimento de célula tronco pluripotente
Philipp et al. Beneficial applications of biofilms
Van Der Sanden et al. Optimizing stem cell culture
Hughes et al. Introduction: biocatalysis in industry
Tseng et al. Spontaneous osteogenesis of MSCs cultured on 3D microcarriers through alteration of cytoskeletal tension
Lü et al. Differential regulation of morphology and stemness of mouse embryonic stem cells by substrate stiffness and topography
Higuchi et al. Generation of pluripotent stem cells without the use of genetic material
Tay et al. Mechanoregulation of stem cell fate via micro-/nano-scale manipulation for regenerative medicine
MX2010002426A (es) Agente para promover la adhesion de las celulas del endotelio corneal.
WO2011159359A3 (en) Serum-free chemically defined cell culture medium
BRPI0619794B8 (pt) Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
EP4368701A3 (en) Feeder-free culture system
Liu et al. Engineering stem cell niches in bioreactors
BR112012026247A2 (pt) método de tratar uma corrente residual tendo componentes anaerobicamente biodegradáveis e biorreator de membrana anaeróbica para biodegradar anaerobicamente os componentes em uma corrente residual
BR112012009520A2 (pt) processo para a cultura de algas
WO2012052854A3 (en) Plant cell lines and methods of isolating the same
WO2011058558A3 (en) Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
SE0850111L (sv) Förfarande för differentiering av stamceller till celler av den endodermala och pankreatiska utvecklingslinjen
BRPI0922572A2 (pt) método para cultivar células pluripotentes, cultura de células de mamífero pluripotentes, meio de cultura de célula, célula animal pluripotente isolada, e, método para aumentar a pluripotência de uma célula de mamífero.
Senko et al. Biogas production from biomass of microalgae Chlorella vulgaris in the presence of benzothiophene sulfone
Doroudian et al. Cyclic strain dominates over microtopography in regulating cytoskeletal and focal adhesion remodeling of human mesenchymal stem cells
DK1720979T3 (da) Anvendelse af et serumfrit celledyrkningsmedium til fremstilling af IL-18BP I pattedyrceller
ATE517979T1 (de) Fermenter zur erzeugung von biogas aus organischem material
Ramírez‐García et al. Unveiling and initial characterization of neural crest‐like cells in mesenchymal populations from the human periodontal ligament
Byrne et al. Characterization and adaptation of Caldicellulosiruptor strains to higher sugar concentrations, targeting enhanced hydrogen production from lignocellulosic hydrolysates

Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: TOSHIHIRO AKAIKE (JP) , KEIICHI FUKUDA (JP) , DAII

Free format text: TRANSFERIDO POR INCORPORACAO DE: ASUBIO PHARMA CO., LTD.

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B25A Requested transfer of rights approved

Owner name: TOSHIHIRO AKAIKE (JP)

B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B15K Others concerning applications: alteration of classification

Ipc: C12N 5/0735 (2010.01), C12N 15/09 (2006.01), C07K

B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]