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BRPI0412535A - serina acetiltransferase mutante, dna, bactéria, e, método para produzir l-cisteìna - Google Patents

serina acetiltransferase mutante, dna, bactéria, e, método para produzir l-cisteìna

Info

Publication number
BRPI0412535A
BRPI0412535A BRPI0412535-5A BRPI0412535A BRPI0412535A BR PI0412535 A BRPI0412535 A BR PI0412535A BR PI0412535 A BRPI0412535 A BR PI0412535A BR PI0412535 A BRPI0412535 A BR PI0412535A
Authority
BR
Brazil
Prior art keywords
cysteine
bacterium
dna
producing
serine acetyltransferase
Prior art date
Application number
BRPI0412535-5A
Other languages
English (en)
Inventor
Tatsuki Kashiwagi
Yuko Kai
Kohki Ishikawa
Ei-Ichiro Suzuki
Hiroshi Takagi
Mikhail Kharisovich Ziyatdinov
Ekaterina Igorevna Redkina
Mikhail Markovich Gusyatiner
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from RU2003121601/13A external-priority patent/RU2003121601A/ru
Priority claimed from RU2003135291/13A external-priority patent/RU2279477C2/ru
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of BRPI0412535A publication Critical patent/BRPI0412535A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"SERINA ACETIlTRANSFERASE MUTANTE, DNA, BACTéRIA, E, MéTODO PARA PRODUZIR L-CISTEìNA". O-acetilserina, L-cisteína e compostos sulfurosos delas derivados, são produzidos mediante o cultivo de bactérias do gênero Escherichia retendo uma acetiltransferase variante que é uma serina acetiltransferase do tipo selvagem tendo a seqüência de aminoácidos correspondente às 89<a> a 96<a> posições substituídas por qualquer uma das seqüências de aminoácidos de SEQ ID NOS: 4 a 9 e tendo a retroinibição pela dessensibilização da L-cisteína.
BRPI0412535-5A 2003-07-16 2004-07-16 serina acetiltransferase mutante, dna, bactéria, e, método para produzir l-cisteìna BRPI0412535A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
RU2003121601/13A RU2003121601A (ru) 2003-07-16 2003-07-16 Мутантная серинацетилтрансфераза
RU2003135291/13A RU2279477C2 (ru) 2003-12-05 2003-12-05 Мутантная серинацетилтрансфераза, фрагмент днк, кодирующий мутантную серинацетилтрансферазу (варианты), бактерия, принадлежащая к роду escherichia, - продуцент l-цистеина, и способ продукции l-цистеина
PCT/JP2004/010216 WO2005007841A1 (ja) 2003-07-16 2004-07-16 変異型セリンアセチルトランスフェラーゼ及びｌ−システインの製造法

Publications (1)

Publication Number Publication Date
BRPI0412535A true BRPI0412535A (pt) 2006-09-19

Family

ID=34082460

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0412535-5A BRPI0412535A (pt) 2003-07-16 2004-07-16 serina acetiltransferase mutante, dna, bactéria, e, método para produzir l-cisteìna

Country Status (6)

Country Link
US (1) US7312058B2 (pt)
EP (1) EP1650296B1 (pt)
JP (1) JP4923573B2 (pt)
AT (1) ATE554162T1 (pt)
BR (1) BRPI0412535A (pt)
WO (1) WO2005007841A1 (pt)

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BR0200350B1 (pt) * 2001-02-13 2014-10-14 Ajinomoto Kk Bactéria transgênica produtora de l-aminoácido pertencente ao gênero escherichia, e, método para produzir l-aminoácido
RU2229513C2 (ru) * 2001-11-23 2004-05-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения l-аминокислот, штамм escherichia coli - продуцент l-аминокислоты (варианты)
JP4479283B2 (ja) * 2004-03-04 2010-06-09 味の素株式会社 Ｌ−システイン生産菌及びｌ−システインの製造法
ATE438731T1 (de) 2005-02-18 2009-08-15 Ajinomoto Kk Verfahren zur herstellung einer l-aminosäure unter verwendung eines bakteriums der familie enterobacteriaceae
WO2006123764A1 (en) * 2005-05-16 2006-11-23 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the kefb gene
WO2007086546A1 (en) * 2006-01-26 2007-08-02 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of enterobacteriaceae family with attenuated expression of the aldh gene
EP2351830B1 (en) 2006-03-23 2014-04-23 Ajinomoto Co., Inc. A method for producing an L-amino acid using bacterium of the Enterobacteriaceae family with attenuated expression of a gene coding for small RNA
WO2007119890A1 (en) * 2006-04-18 2007-10-25 Ajinomoto Co., Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE sfmACDFH-fimZ CLUSTER OR THE fimZ GENE
WO2007139219A1 (en) * 2006-06-01 2007-12-06 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the rcsa gene
RU2337961C2 (ru) * 2006-07-04 2008-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПОЛУЧЕНИЯ L-ТРЕОНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia, В КОТОРОЙ ИНАКТИВИРОВАН ОПЕРОН rspAB
RU2006143864A (ru) * 2006-12-12 2008-06-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНОВ cynT, cynS, cynX, ИЛИ cynR, ИЛИ ИХ КОМБИНАЦИИ
DE102007007333A1 (de) 2007-02-14 2008-08-21 Wacker Chemie Ag Verfahren zur Reinigung von L-Cystein
KR101162049B1 (ko) * 2007-02-15 2012-07-04 아지노모토 가부시키가이샤 디설파이드 결합 도입 트랜스글루타미나제
EP2239335B1 (en) * 2008-01-18 2013-02-27 Ajinomoto Co., Inc. Process for production of serine derivative, and protein for use in the process
EP2246420B1 (en) * 2008-02-21 2014-12-31 Ajinomoto Co., Inc. L-cysteine-producing bacterium, and method for production of l-cysteine
DE602009000714D1 (de) * 2008-03-06 2011-03-24 Ajinomoto Kk L-Zystein-produzierendes Bakterium und Verfahren zur Herstellung von L-Zystein
JP5332237B2 (ja) * 2008-03-06 2013-11-06 味の素株式会社 Ｌ−システイン生産菌及びｌ−システインの製造法
PE20110369A1 (es) 2008-09-08 2011-06-24 Ajinomoto Kk Un microorganismo que produce l-aminoacido y un metodo para producir un l-aminoacido
RU2008148283A (ru) * 2008-12-09 2010-06-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АРГИНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА artI
JP5521347B2 (ja) 2009-02-16 2014-06-11 味の素株式会社 Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法
JP5463528B2 (ja) * 2009-02-25 2014-04-09 味の素株式会社 Ｌ−システイン生産菌及びｌ−システインの製造法
JP5359409B2 (ja) 2009-03-12 2013-12-04 味の素株式会社 Ｌ−システイン生産菌及びｌ−システインの製造法
RU2009136544A (ru) 2009-10-05 2011-04-10 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) (RU) Способ получения l-цистеина с использованием бактерии семейства enterobacteriaceae
JP5817529B2 (ja) 2009-11-30 2015-11-18 味の素株式会社 Ｌ−システイン生産菌及びｌ−システインの製造法
RU2460793C2 (ru) 2010-01-15 2012-09-10 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) Способ получения l-аминокислот с использованием бактерий семейства enterobacteriaceae
WO2012036151A1 (ja) 2010-09-14 2012-03-22 味の素株式会社 含硫アミノ酸生産菌及び含硫アミノ酸の製造法
JP2014087259A (ja) 2011-02-22 2014-05-15 Ajinomoto Co Inc Ｌ−システイン生産菌及びｌ−システインの製造法
US9234223B2 (en) 2011-04-01 2016-01-12 Ajinomoto Co., Inc. Method for producing L-cysteine
CN104160024A (zh) 2011-04-01 2014-11-19 味之素株式会社 用于生产l-半胱氨酸的方法
JP2014131487A (ja) * 2011-04-18 2014-07-17 Ajinomoto Co Inc Ｌ−システインの製造法
PE20150681A1 (es) 2013-05-13 2015-05-15 Ajinomoto Kk Metodo para producir l-aminoacidos
JP2016165225A (ja) 2013-07-09 2016-09-15 味の素株式会社 有用物質の製造方法
ES2694011T3 (es) 2013-10-21 2018-12-17 Ajinomoto Co., Inc. Método para producir L-aminoácido
JP7066977B2 (ja) 2017-04-03 2022-05-16 味の素株式会社 Ｌ－アミノ酸の製造法
KR20190092950A (ko) 2018-01-31 2019-08-08 씨제이제일제당 (주) 연속식 크로마토그래피 공정을 이용한 천연 l-시스테인 염산염 수화물 결정의 제조 방법
KR20190092951A (ko) 2018-01-31 2019-08-08 씨제이제일제당 (주) 연속식 크로마토그래피 공정을 이용한 천연 l-시스테인 결정의 제조 방법
EP3861109A1 (en) 2018-10-05 2021-08-11 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation
BR112021014194A2 (pt) 2019-02-22 2021-12-28 Ajinomoto Kk Método para a produção de um l-aminoácido
BR112021017870A2 (pt) 2019-04-05 2021-12-07 Ajinomoto Kk Método para produzir um l-aminoácido
WO2021060438A1 (en) 2019-09-25 2021-04-01 Ajinomoto Co., Inc. Method for producing l-amino acids by bacterial fermentation
KR102277404B1 (ko) * 2021-01-27 2021-07-14 씨제이제일제당 주식회사 신규한 갈락토사이드 o-아세틸트랜스퍼라제 변이체 및 이를 이용한 l-글루탐산 생산 방법
WO2025072960A2 (en) * 2023-09-29 2025-04-03 Northeastern University Methods to modulate in vivo levels of hydrogen sulfide by engineered organisms

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Also Published As

Publication number Publication date
JPWO2005007841A1 (ja) 2006-11-09
EP1650296A4 (en) 2006-07-12
WO2005007841A1 (ja) 2005-01-27
EP1650296A1 (en) 2006-04-26
ATE554162T1 (de) 2012-05-15
US7312058B2 (en) 2007-12-25
JP4923573B2 (ja) 2012-04-25
EP1650296B1 (en) 2012-04-18
US20050112731A1 (en) 2005-05-26

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