BRPI0419151A - método para a produção em massa de lectina ligante de manose multimérica - Google Patents
método para a produção em massa de lectina ligante de manose multiméricaInfo
- Publication number
- BRPI0419151A BRPI0419151A BRPI0419151-0A BRPI0419151A BRPI0419151A BR PI0419151 A BRPI0419151 A BR PI0419151A BR PI0419151 A BRPI0419151 A BR PI0419151A BR PI0419151 A BRPI0419151 A BR PI0419151A
- Authority
- BR
- Brazil
- Prior art keywords
- mbl
- production
- cell line
- relates
- multimeric
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 title 1
- 102000004856 Lectins Human genes 0.000 title 1
- 108090001090 Lectins Proteins 0.000 title 1
- 239000002523 lectin Substances 0.000 title 1
- 239000003446 ligand Substances 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 4
- 101710110798 Mannose-binding protein C Proteins 0.000 abstract 3
- 102100026553 Mannose-binding protein C Human genes 0.000 abstract 3
- 101001056128 Homo sapiens Mannose-binding protein C Proteins 0.000 abstract 2
- 238000010276 construction Methods 0.000 abstract 2
- 208000035143 Bacterial infection Diseases 0.000 abstract 1
- 108091026890 Coding region Proteins 0.000 abstract 1
- 206010017533 Fungal infection Diseases 0.000 abstract 1
- 208000031888 Mycoses Diseases 0.000 abstract 1
- 208000036142 Viral infection Diseases 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 208000022362 bacterial infectious disease Diseases 0.000 abstract 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000004017 serum-free culture medium Substances 0.000 abstract 1
- 229940124597 therapeutic agent Drugs 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4726—Lectins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4724—Lectins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Biochemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
MéTODO PARA A PRODUçãO EM MASSA DE LECTINA LIGANTE DE MANOSE MULTIMéRICA. Trata-se de um método paira a produção em massa de lectina ligante de manose humana recombinante multimérica (rhMBL) em uma linhagem de células CHO transfectada com um vetor contendo a seqüência da região codificadora de MBL humana. Ainda, esta invenção refere-se à construção de uma linhagem de células recombinantes da qual MBL pode ser produzida mais que 80% como forma polimérica altamente funcional. Ainda, essa invenção refere-se à construção de uma linhagem de células recombinantes que pode produzir 50 <109>g/milhão de células/dia em um sistema de cultura em frasco para a produção de MBL. Ainda, esta invenção descreve métodos para cultura de alta densidade da célula em um meio isento de proteína usando biorreator e para a purificação seletiva da forma de alto peso molecular da MBL do meio de cultura. Essa invenção descreve também um método para a produção de rhMBL funcional que pode ser usada no desenvolvimento de um agente terapêutico para o tratamento de infecções virais, bacterianas, ou fúngicas.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/KR2004/002739 WO2006046786A1 (en) | 2004-10-28 | 2004-10-28 | Method for the mass production of multimeric mannose binding lectin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0419151A true BRPI0419151A (pt) | 2007-12-11 |
Family
ID=36228005
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0419151-0A BRPI0419151A (pt) | 2004-10-28 | 2004-10-28 | método para a produção em massa de lectina ligante de manose multimérica |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP1812459B1 (pt) |
| JP (1) | JP4592756B2 (pt) |
| AT (1) | ATE503766T1 (pt) |
| BR (1) | BRPI0419151A (pt) |
| DE (1) | DE602004032062D1 (pt) |
| DK (1) | DK1812459T3 (pt) |
| ES (1) | ES2360504T3 (pt) |
| MX (1) | MX2007005107A (pt) |
| PL (1) | PL1812459T3 (pt) |
| WO (1) | WO2006046786A1 (pt) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100836745B1 (ko) * | 2007-01-31 | 2008-06-10 | (주)두비엘 | Ｈｂｖ 백신 및 그의 제조 방법 |
| JP5071940B2 (ja) * | 2008-12-25 | 2012-11-14 | 独立行政法人産業技術総合研究所 | 抗真菌剤 |
| US9150631B2 (en) | 2010-01-19 | 2015-10-06 | President And Fellows Of Harvard College | Engineered opsonin for pathogen detection and treatment |
| EP3081937B1 (en) | 2011-07-18 | 2019-11-13 | President and Fellows of Harvard College | Engineered microbe-targeting molecules and uses thereof |
| US9632085B2 (en) | 2012-02-29 | 2017-04-25 | President And Fellows Of Harvard College | Rapid antibiotic susceptibility testing |
| EP2976642A4 (en) | 2013-03-15 | 2016-09-21 | Harvard College | METHOD AND COMPOSITIONS FOR IMPROVING THE DETECTION AND / OR RECORDING OF A TARGET UNIT |
| JP6649250B2 (ja) | 2013-05-21 | 2020-02-19 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 操作されたヘム結合性構成物およびその使用 |
| US10513546B2 (en) | 2013-12-18 | 2019-12-24 | President And Fellows Of Harvard College | CRP capture/detection of gram positive bacteria |
| EP3763378A1 (en) | 2015-08-06 | 2021-01-13 | President and Fellows of Harvard College | Improved microbe-binding molecules and uses thereof |
| CA3207352A1 (en) | 2021-02-08 | 2022-08-11 | Alberto Mantovani | Molecules for use in the treatment and/or prevention of covid-19 |
| CN118027168B (zh) * | 2024-03-19 | 2024-08-06 | 广东现代汉方科技有限公司 | 基于真核表达的msl重组植物蛋白的制备方法及用途 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6337193B1 (en) * | 1998-11-24 | 2002-01-08 | Aptagen, Inc. | Expression of manose-binding protein in methylotrophic yeast |
| US6589534B1 (en) * | 1999-09-30 | 2003-07-08 | Yeda Research And Development Co., Ltd. | Hepatitis B virus binding proteins and uses thereof |
| KR100408844B1 (ko) * | 2000-07-29 | 2003-12-06 | 한국산업기술평가원 | 동물세포 발현벡터 |
| CN1549823A (zh) * | 2001-07-23 | 2004-11-24 | 纳蒂芒公司 | 高分子量凝集素的生产 |
| JP4051030B2 (ja) * | 2001-08-31 | 2008-02-20 | 扶桑薬品工業株式会社 | ヒトマンノース結合レクチンの精製方法、ヒトマンノース結合レクチン組成物およびヒトマンノース結合レクチンの医薬用途 |
| KR100755931B1 (ko) * | 2003-06-11 | 2007-09-06 | (주)두비엘 | 고분자형 만노스 결합형 렉틴의 대량 생산방법 및 상기방법으로 제조된 만노스 결합형 렉틴 재조합 단백질의제제화 |
-
2004
- 2004-10-28 MX MX2007005107A patent/MX2007005107A/es active IP Right Grant
- 2004-10-28 JP JP2007538806A patent/JP4592756B2/ja not_active Expired - Lifetime
- 2004-10-28 WO PCT/KR2004/002739 patent/WO2006046786A1/en not_active Ceased
- 2004-10-28 DE DE602004032062T patent/DE602004032062D1/de not_active Expired - Lifetime
- 2004-10-28 PL PL04793596T patent/PL1812459T3/pl unknown
- 2004-10-28 EP EP04793596A patent/EP1812459B1/en not_active Expired - Lifetime
- 2004-10-28 AT AT04793596T patent/ATE503766T1/de not_active IP Right Cessation
- 2004-10-28 BR BRPI0419151-0A patent/BRPI0419151A/pt not_active IP Right Cessation
- 2004-10-28 ES ES04793596T patent/ES2360504T3/es not_active Expired - Lifetime
- 2004-10-28 DK DK04793596.0T patent/DK1812459T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| DE602004032062D1 (de) | 2011-05-12 |
| DK1812459T3 (da) | 2011-05-23 |
| EP1812459B1 (en) | 2011-03-30 |
| PL1812459T3 (pl) | 2011-09-30 |
| JP2008517619A (ja) | 2008-05-29 |
| WO2006046786A1 (en) | 2006-05-04 |
| JP4592756B2 (ja) | 2010-12-08 |
| EP1812459A4 (en) | 2008-10-01 |
| ES2360504T3 (es) | 2011-06-06 |
| ATE503766T1 (de) | 2011-04-15 |
| EP1812459A1 (en) | 2007-08-01 |
| MX2007005107A (es) | 2007-09-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 11A ANUIDADE. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2329 DE 25-08-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |