BR9910414A - Isolated protein, isolated nucleic acid, vector for the expression of a psidium guajava 13-hydroperoxide lyase, cell containing an exogenous nucleic acid, and processes for cleaving a 13-hydroperoxide of linoleic acid or an alpha-linolenic acid in a c6 aldehyde and a c12 oxocarboxylic acid, to prepare n-hexanal, 3- (z) -hexen-1-al, 2- (e) -hexen-1-al or its corresponding alcohols, and to express a recombinant protein - Google Patents
Isolated protein, isolated nucleic acid, vector for the expression of a psidium guajava 13-hydroperoxide lyase, cell containing an exogenous nucleic acid, and processes for cleaving a 13-hydroperoxide of linoleic acid or an alpha-linolenic acid in a c6 aldehyde and a c12 oxocarboxylic acid, to prepare n-hexanal, 3- (z) -hexen-1-al, 2- (e) -hexen-1-al or its corresponding alcohols, and to express a recombinant proteinInfo
- Publication number
- BR9910414A BR9910414A BR9910414-8A BR9910414A BR9910414A BR 9910414 A BR9910414 A BR 9910414A BR 9910414 A BR9910414 A BR 9910414A BR 9910414 A BR9910414 A BR 9910414A
- Authority
- BR
- Brazil
- Prior art keywords
- acid
- hexen
- hydroperoxide
- nucleic acid
- protein
- Prior art date
Links
- 108010064894 hydroperoxide lyase Proteins 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 240000001679 Psidium guajava Species 0.000 title abstract 3
- 235000013929 Psidium pyriferum Nutrition 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 2
- 239000002253 acid Substances 0.000 title abstract 2
- 150000001298 alcohols Chemical class 0.000 title abstract 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 title 2
- JARKCYVAAOWBJS-UHFFFAOYSA-N hexanal Chemical compound CCCCCC=O JARKCYVAAOWBJS-UHFFFAOYSA-N 0.000 title 2
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 title 1
- 150000001299 aldehydes Chemical class 0.000 title 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 title 1
- 235000020778 linoleic acid Nutrition 0.000 title 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 title 1
- 229960004488 linolenic acid Drugs 0.000 title 1
- 241000508269 Psidium Species 0.000 abstract 3
- VRAIHTAYLFXSJJ-UHFFFAOYSA-N alumane Chemical compound [AlH3].[AlH3] VRAIHTAYLFXSJJ-UHFFFAOYSA-N 0.000 abstract 1
- 235000014113 dietary fatty acids Nutrition 0.000 abstract 1
- 229930195729 fatty acid Natural products 0.000 abstract 1
- 239000000194 fatty acid Substances 0.000 abstract 1
- 150000004665 fatty acids Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
"PROTEìNA ISOLADA, áCIDO NUCLEICO ISOLADO, VETOR PARA A EXPRESSãO DE UMA 13-HIDROPERóXIDO LIASE DE PSIDIUM GUAJAVA, CéLULA CONTENDO UM áCIDO NUCLEICO EXóGENO, E, PROCESSOS PARA CLIVAR UM 13-HIDROPERóXIDO DE áCIDO LINOLEICO OU DE UM ALFA-LINOLêNICO EM UM ALDEìDO C6 E UM áCIDO OXOCARBOXìLICO C12, PARA PREPARAR N-HEXANAL, 3-(Z)-HEXEN-1-AL, 2-(E)-HEXEN-1-AL OU SEUS áLCOOIS CORRESPONDENTES, E PARA EXPRESSAR UMA PROTEìNA RECOMBINANTE". A presente invenção diz respeito a um ácido graxo da proteína 13-hidroperóxido liase de goiaba (Psidium guajava) e ao gene que codifica a protéina. Sistemas de expressão para a 13-hidroperóxido liase recombinante da goiaba e processos de usar 13-hidroperóxido liase recombinante da goiaba para a produção de caráteres verdes são fornecidos."ISOLATED PROTEIN, ISOLATED NUCLEIC ACID, VECTOR FOR THE EXPRESSION OF A 13-HYDROPEROXIDE LIASE FROM PSIDIUM GUAJAVA, CELL CONTAINING AN EXOGENOUS NUCLEIC ACID, AND PROCESSES TO CLAIM A 13-HYDROPEROXIC ALUMIN ALUMINUM LINEN C6 AND A C12 OXOCARBOXYLIC ACID, TO PREPARE N-HEXANAL, 3- (Z) -HEXEN-1-AL, 2- (E) -HEXEN-1-AL OR ITS CORRESPONDING ALCOHOLS, AND TO EXPRESS A RECOMBINANT PROTEIN ". The present invention concerns a fatty acid of guava 13-hydroperoxide lyase (Psidium guajava) and the gene encoding the protein. Expression systems for the recombinant guava 13-hydroperoxide lyase and processes for using recombinant guava 13-hydroperoxide lyase for the production of green characters are provided.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/078,173 US6200794B1 (en) | 1998-05-13 | 1998-05-13 | Guava (Psidium guajava) 13-hydroperoxide lyase and uses thereof |
| PCT/IB1999/000807 WO1999058648A2 (en) | 1998-05-13 | 1999-05-05 | Guava (psidium guajava) 13-hydroperoxide lyase and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR9910414A true BR9910414A (en) | 2001-09-04 |
| BR9910414B1 BR9910414B1 (en) | 2012-09-18 |
Family
ID=22142395
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI9910414-8A BR9910414B1 (en) | 1998-05-13 | 1999-05-05 | transgenic escherichia coli or yeast cell, and process for cleaving a 13-hydroperoxide of linoleic or alpha-linolenic acid into a c6 aldehyde and a c12 oxocarboxylic acid. |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6200794B1 (en) |
| EP (1) | EP1080205B1 (en) |
| BR (1) | BR9910414B1 (en) |
| DE (1) | DE69938343T2 (en) |
| ES (1) | ES2301238T3 (en) |
| WO (1) | WO1999058648A2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000022145A2 (en) * | 1998-10-13 | 2000-04-20 | Pioneer Hi-Bred International, Inc. | A hydroperoxide lyase gene from maize and methods of use |
| US6271018B1 (en) * | 2000-03-29 | 2001-08-07 | Vanderbilt University | Muskmelon (Cucumis melo) hydroperoxide lyase and uses thereof |
| DE10028639C2 (en) * | 2000-06-09 | 2003-11-06 | Ipk Inst Fuer Pflanzengenetik | Process for the preparation of C9 aldehydes, C9 alcohols and their esters |
| US20040010822A1 (en) * | 2002-05-10 | 2004-01-15 | Mcgonigle Brian | Hydroperoxyde lyases |
| PL2167658T3 (en) * | 2007-06-28 | 2013-04-30 | Firmenich & Cie | Modified 13-hydroperoxide lyases and uses thereof |
| CN120005901A (en) * | 2025-01-21 | 2025-05-16 | 浙江大学 | A tomato SlMET1L gene and its application in regulating fruit ripening and preservation quality |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69320302T2 (en) | 1992-05-26 | 1999-01-28 | Dean, Christopher, Grand-Lancy | Enzymatic process for the preparation of n-hexanal, 3- (Z) -hexen-1-al, 2- (E) -hexen-1-al, or corresponding alcohols using a soybean lipoxygenase and a guava lyase |
| EP0801133B1 (en) | 1996-04-15 | 2004-09-08 | Givaudan SA | Hydroperoxide lyases |
| CA2301856A1 (en) | 1998-06-26 | 2000-01-06 | Calgene Llc | Fatty acid hydroperoxide lyase nucleic acid sequences |
-
1998
- 1998-05-13 US US09/078,173 patent/US6200794B1/en not_active Expired - Lifetime
-
1999
- 1999-05-05 ES ES99915975T patent/ES2301238T3/en not_active Expired - Lifetime
- 1999-05-05 DE DE69938343T patent/DE69938343T2/en not_active Expired - Lifetime
- 1999-05-05 BR BRPI9910414-8A patent/BR9910414B1/en not_active IP Right Cessation
- 1999-05-05 EP EP99915975A patent/EP1080205B1/en not_active Expired - Lifetime
- 1999-05-05 WO PCT/IB1999/000807 patent/WO1999058648A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| DE69938343D1 (en) | 2008-04-24 |
| US6200794B1 (en) | 2001-03-13 |
| ES2301238T3 (en) | 2008-06-16 |
| EP1080205B1 (en) | 2008-03-12 |
| WO1999058648A2 (en) | 1999-11-18 |
| DE69938343T2 (en) | 2009-03-26 |
| EP1080205A2 (en) | 2001-03-07 |
| WO1999058648A3 (en) | 2000-02-24 |
| BR9910414B1 (en) | 2012-09-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CL2021002740A1 (en) | A prevotella and francisella crispr protein 1 (cpf1) isolated from acidaminococcus sp. bv3l6(ascpf1); fusion protein comprising it; nucleic acid; vector; methods for altering the genome of a cell or a dsna molecule in vitro using said proteins (divisional de sol. 201903004) | |
| NO20101157L (en) | FAD4, FAD5, FAD5-2 and FAD6, new fatty acid desaturase family members and uses thereof | |
| WO2002008388A3 (en) | Stem cell-like cells | |
| AU2220800A (en) | Compositions and methods for packaging of alphavirus vectors | |
| BR0015631A (en) | Plant, polynucleotide, vector, cell, composition, polypeptide, methods for producing a plant and for identifying a factor that is modulated by, or interacts with, a polypeptide encoded by a polynucleotide, a molecule that modulates the activity or expression of a polynucleotide or polypeptide of interest, and a sequence similar or homologous to one or more polynucleotides, and, integrated system, computerized or legible in computer | |
| IL164803A0 (en) | Means and methods for the production of adenovirusvectors | |
| BR9811865A (en) | Isolation of a new senescence factor gene, p23 | |
| AR004668A1 (en) | A PURIFIED ISOLATED NUCLEIC ACID, A RECOMBINING VECTOR OF EXPRESSION THAT UNDERSTANDS IT, A CELL THAT INCLUDES SUCH A VECTOR, A METHOD FOR THE PRODUCTION OF A H.PYLORI POLYPEPTIDE, A COMPOSITION OF A VACCINE, A PREPARED COMPOSITION PURIFIED BY H. | |
| BR0116033A (en) | Isothermal amplification of nucleic acids on a solid support | |
| MX428A (en) | REGULATORY REGION FOR EXPRESSION OF HETEROLOGY GENE IN YEAST. | |
| ES8800726A1 (en) | METHOD FOR THE MANUFACTURE OF A TEST FOR THE DETECTION OF D.N.A. OF A LYMPHADENOCYTE VIRUS. | |
| BR9813608A (en) | Isolated polypeptide, isolated and mutant nucleic acid sequences, nucleic acid construction, recombinant expression vector, recombinant host cell, processes for producing a mutant nucleic acid sequence, for producing a polypeptide, for producing a mutant of an originating cell and for produce a protein, and, mutant cell | |
| BR9910414A (en) | Isolated protein, isolated nucleic acid, vector for the expression of a psidium guajava 13-hydroperoxide lyase, cell containing an exogenous nucleic acid, and processes for cleaving a 13-hydroperoxide of linoleic acid or an alpha-linolenic acid in a c6 aldehyde and a c12 oxocarboxylic acid, to prepare n-hexanal, 3- (z) -hexen-1-al, 2- (e) -hexen-1-al or its corresponding alcohols, and to express a recombinant protein | |
| DE69933550D1 (en) | MODIFIED ADENOVIRES CONTAINING A FIBER SPARE PROTEIN | |
| BR0016867A (en) | Insulin analog precursor, polynucleotide sequence, expression vector, host cell, and processes for making an insulin analog precursor and for making an insulin analog | |
| ATE319848T1 (en) | PRODUCTION OF POLYKETIDES | |
| BR0103240A (en) | Process for the production of a target fermentation product | |
| ZA202501953B (en) | Masking polypeptide, activatable novel prodrugs and methods of use thereof | |
| DK0953644T3 (en) | Systems for mass production of proteins or peptides by microorganisms of the genus Humicola | |
| BR9811850A (en) | Recombinant expression of insulin c peptide | |
| BRPI0414670A (en) | method for preparing a cell, cell, methods for providing a cell and for generating a batch of recombinant adenovirus, recombinant molecule, assembly of at least two nucleic acid molecules, and packaging system | |
| BR0316510A (en) | Expression vector, process for the preparation of heterologous gene products and selection process for high yield recombinant cells | |
| ATE203770T1 (en) | DNA COMPOSITION AND MODIFIED YEAST | |
| Lithgow et al. | Identification of a GTP-binding protein in the contact sites between inner and outer mitochondrial membranes | |
| DE59913079D1 (en) | RECOMBINANT MISTLE LEMONS |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B15K | Others concerning applications: alteration of classification |
Free format text: ALTERADA DE C12N 9/00. Ipc: C12N 9/88 (2009.01), C12P 7/42 (2009.01) |
|
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 18/09/2012, OBSERVADAS AS CONDICOES LEGAIS. |
|
| B21A | Patent or certificate of addition expired [chapter 21.1 patent gazette] |
Free format text: PATENTE EXTINTA EM 18/09/2022 |