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BR9811542A - Identificação de linhas de células humanas para a produção de proteìnas humanas mediante ativação de genes endógena - Google Patents

Identificação de linhas de células humanas para a produção de proteìnas humanas mediante ativação de genes endógena

Info

Publication number
BR9811542A
BR9811542A BR9811542-1A BR9811542A BR9811542A BR 9811542 A BR9811542 A BR 9811542A BR 9811542 A BR9811542 A BR 9811542A BR 9811542 A BR9811542 A BR 9811542A
Authority
BR
Brazil
Prior art keywords
human
identification
production
human proteins
cell lines
Prior art date
Application number
BR9811542-1A
Other languages
English (en)
Inventor
Michael Brandt
Reinhard Franze
Ulrich Pessara
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27238256&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR9811542(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US09/113,692 external-priority patent/US6548296B1/en
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of BR9811542A publication Critical patent/BR9811542A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Patente de Invenção:<B>"IDENTIFICAçãO DE LINHAS DE CéLULAS HUMANAS PARA A PRODUçãO DE PROTEìNAS HUMANAS MEDIANTE ATIVAçãO DE GENES ENDóGENA"<D>. A invenção refere-se a processos para a escolha de células humanas para a preparação de proteínas humanas mediante ativação de gene endógena, para produzir proteínas humanas em rendimentos económicos e em uma forma, que é adequada para a preparação de uma preparado farmacêutico. Além disso, a invenção refere-se a um processo para a preparação de proteínas humanas em uma linha de células identificadas desta maneira.
BR9811542-1A 1997-07-23 1998-07-22 Identificação de linhas de células humanas para a produção de proteìnas humanas mediante ativação de genes endógena BR9811542A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP97112640 1997-07-23
EP97121073 1997-12-01
US09/113,692 US6548296B1 (en) 1997-07-23 1998-07-10 Methods for identifying human cell lines useful for endogenous gene activation, isolated human lines identified thereby, and uses thereof
PCT/EP1998/004584 WO1999005267A1 (de) 1997-07-23 1998-07-22 Identifizierung humaner zellinien zur produktion humaner proteine durch endogene genaktivierung

Publications (1)

Publication Number Publication Date
BR9811542A true BR9811542A (pt) 2000-08-22

Family

ID=27238256

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9811542-1A BR9811542A (pt) 1997-07-23 1998-07-22 Identificação de linhas de células humanas para a produção de proteìnas humanas mediante ativação de genes endógena

Country Status (14)

Country Link
US (1) US6395484B1 (pt)
EP (1) EP1000154B1 (pt)
JP (1) JP2001511342A (pt)
CN (1) CN1150319C (pt)
AT (1) ATE354654T1 (pt)
AU (1) AU737605B2 (pt)
BR (1) BR9811542A (pt)
CA (1) CA2298412C (pt)
DE (1) DE59813913D1 (pt)
DK (1) DK1000154T3 (pt)
ES (1) ES2281133T3 (pt)
TR (1) TR200000140T2 (pt)
TW (1) TWI224140B (pt)
WO (1) WO1999005267A1 (pt)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100622203B1 (ko) * 1997-07-23 2006-09-07 로셰 디아그노스틱스 게엠베하 내인성 유전자 활성화에 의해 사람 단백질을 생성시키는 사람 세포주를 확인하는 방법
GB0200977D0 (en) * 2002-01-17 2002-03-06 Lonza Biologics Plc Glutamine-auxotrophic human cells capable of producing proteins and capable of growing in a glutamine-free medium
AU2003226960B2 (en) * 2002-01-17 2007-11-22 Lonza Biologics Plc. Glutamine-auxothrophic human cells capable of producing proteins and capable of growing in a glutamine-free medium
GB0208041D0 (en) * 2002-04-08 2002-05-22 Lonza Biologics Plc Method of culturing animal cells
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
WO2005083060A1 (en) 2004-03-01 2005-09-09 Kitakyushu Foundation For The Advancement Of Industry, Science And Technology Human cell strains for protein production, provided by selecting strains with high intracellular protein and mutating with carcinogens
DE102012009849A1 (de) 2012-05-21 2013-11-21 Hartwig Schwieger Schwingungsstabilisierte Windenergieanlage-Zug-Druck-Knoten
JP6652334B2 (ja) 2014-05-31 2020-02-19 Ｊｃｒファーマ株式会社 ウリジンとｎ−アセチル−ｄ−マンノサミンとを含有する培地
US11377652B2 (en) * 2018-07-09 2022-07-05 NanoCav, LLC Micro flow-through electroporation devices and methods of cell transfection
US12188042B2 (en) 2018-07-09 2025-01-07 NanoCav, LLC Electroporation devices and methods of cell transfection

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL77081A (en) * 1984-12-04 1999-10-28 Genetics Inst Dna sequence encoding human erythropoietin process for the preparation thereof and a pharmaceutical composition of human erythropoietin
JPS62171696A (ja) 1986-01-23 1987-07-28 Sumitomo Chem Co Ltd ヒトエリスロポエチンの製造方法
US4954437A (en) 1986-09-15 1990-09-04 Integrated Genetics, Inc. Cell encoding recombinant human erythropoietin
JP2879906B2 (ja) * 1989-09-14 1999-04-05 鐘紡株式会社 プロコラゲナーゼの製造方法
ES2127458T3 (es) * 1989-11-06 1999-04-16 Cell Genesys Inc Produccion de proteinas utilizando recombinacion homologa.
US5328844A (en) * 1990-05-09 1994-07-12 University Of Colorado Foundation, Inc. Culture media for mammalian cells
PT101031B (pt) * 1991-11-05 2002-07-31 Transkaryotic Therapies Inc Processo para o fornecimento de proteinas por terapia genetica
US6270989B1 (en) 1991-11-05 2001-08-07 Transkaryotic Therapies, Inc. Protein production and delivery
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
US5449015A (en) * 1994-05-12 1995-09-12 Petkovic; Tony Shock-activated shut-off valve

Also Published As

Publication number Publication date
CA2298412C (en) 2010-10-19
AU737605B2 (en) 2001-08-23
CA2298412A1 (en) 1999-02-04
EP1000154A1 (de) 2000-05-17
JP2001511342A (ja) 2001-08-14
TR200000140T2 (tr) 2000-05-22
US6395484B1 (en) 2002-05-28
DK1000154T3 (da) 2007-06-18
CN1150319C (zh) 2004-05-19
EP1000154B1 (de) 2007-02-21
DE59813913D1 (de) 2007-04-05
AU9067498A (en) 1999-02-16
ES2281133T3 (es) 2007-09-16
WO1999005267A1 (de) 1999-02-04
TWI224140B (en) 2004-11-21
ATE354654T1 (de) 2007-03-15
CN1265144A (zh) 2000-08-30

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Legal Events

Date Code Title Description
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 04/11/2008, OBSERVADAS AS CONDICOES LEGAIS.

B21A Patent or certificate of addition expired [chapter 21.1 patent gazette]

Free format text: PATENTE EXTINTA EM 04.11.2018