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BR9807579A - Aparelhagem de fluxo contìnuo para separação de substâncias carregadas em meio lìquido por focalização isoelétrica, processo para separação ou de purificação de uma substância a partir de uma mistura aquosa, substância purificada, vacina ou kit para teste, enzima hidrolìtica purificada, e, usos de uma enzima e de fetilase. - Google Patents

Aparelhagem de fluxo contìnuo para separação de substâncias carregadas em meio lìquido por focalização isoelétrica, processo para separação ou de purificação de uma substância a partir de uma mistura aquosa, substância purificada, vacina ou kit para teste, enzima hidrolìtica purificada, e, usos de uma enzima e de fetilase.

Info

Publication number
BR9807579A
BR9807579A BR9807579A BR9807579A BR9807579A BR 9807579 A BR9807579 A BR 9807579A BR 9807579 A BR9807579 A BR 9807579A BR 9807579 A BR9807579 A BR 9807579A BR 9807579 A BR9807579 A BR 9807579A
Authority
BR
Brazil
Prior art keywords
substance
purified
separating
enzyme
flow
Prior art date
Application number
BR9807579A
Other languages
English (en)
Inventor
Gerben Foppe De Boer
Otto Sova
Original Assignee
Cerberus Enterprises Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cerberus Enterprises Bv filed Critical Cerberus Enterprises Bv
Publication of BR9807579A publication Critical patent/BR9807579A/pt

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/02—Preparations for cleaning the hair
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
    • C02F1/469—Treatment of water, waste water, or sewage by electrochemical methods by electrochemical separation, e.g. by electro-osmosis, electrodialysis, electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44769—Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2301/00—General aspects of water treatment
    • C02F2301/02—Fluid flow conditions
    • C02F2301/022—Laminar

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Veterinary Medicine (AREA)
  • Analytical Chemistry (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Epidemiology (AREA)
  • Organic Chemistry (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • General Chemical & Material Sciences (AREA)
  • Birds (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Water Treatment By Electricity Or Magnetism (AREA)

Abstract

UBSTâNCIAS CARREGADAS EM MEIO LìQUIDO POR FOCALIZAA AQUOSA, PARA FABRICAçãO DE VACINA E PARA FABRICAenção fornece uma aparelhagem de fluxo contínuo e um processo para purificação de substâncias carreg focalização isoelétrica em uma escala industrial. A aparelhagem compreende uma câmara de separação, um par de eletrodos verticais (9) localizados nos extremos da aparelhagem, uma membrana anión- ou uum dos eletrodos (9) para separar os espaços do eletrodo de uma parte central da aparelhagem, um ou mais orifícios de saída de fluxo (12) para separar as frações líquidas na parte superior da câmara, dois orifícios secundários de saída de fluxo (11) estando situados na parte superior de cada um dos espaços do eletrodo, uma ou mais parede (s) vertical (ais) (6) entre cada um dos ditos orifícios de saída de fluxo que dividem a câmara pelo menos na altura dos ditos orifícios de saída de fluxo (11, 12) e um grande número de paredes divisórias verticais permeáveis (13) para permitir um fluxo ascendente isento de convecção. A aparelhagem da invenção não requer soluções tamponadoras anfóteras adicionais para estabelecer um gradiente adequado de pH e pode ser usada para a purificação de polinucleotídeos, de aminoácidos, de peptídeos, de proteínas, de solventes orgânicos e de bebidas.
BR9807579A 1997-02-20 1998-02-20 Aparelhagem de fluxo contìnuo para separação de substâncias carregadas em meio lìquido por focalização isoelétrica, processo para separação ou de purificação de uma substância a partir de uma mistura aquosa, substância purificada, vacina ou kit para teste, enzima hidrolìtica purificada, e, usos de uma enzima e de fetilase. BR9807579A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97200489 1997-02-20
PCT/NL1998/000104 WO1998036821A1 (en) 1997-02-20 1998-02-20 Method and apparatus for continuous flow isoelectric focusing for purifying biological substances

Publications (1)

Publication Number Publication Date
BR9807579A true BR9807579A (pt) 2000-03-21

Family

ID=8228030

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9807579A BR9807579A (pt) 1997-02-20 1998-02-20 Aparelhagem de fluxo contìnuo para separação de substâncias carregadas em meio lìquido por focalização isoelétrica, processo para separação ou de purificação de uma substância a partir de uma mistura aquosa, substância purificada, vacina ou kit para teste, enzima hidrolìtica purificada, e, usos de uma enzima e de fetilase.

Country Status (16)

Country Link
US (2) US6572751B1 (pt)
EP (1) EP0963237B1 (pt)
JP (1) JP2001513013A (pt)
KR (1) KR100493975B1 (pt)
CN (1) CN1245246C (pt)
AP (1) AP9901607A0 (pt)
AT (1) ATE241422T1 (pt)
AU (1) AU739328B2 (pt)
BR (1) BR9807579A (pt)
CA (1) CA2281867A1 (pt)
DE (1) DE69815069T2 (pt)
IL (1) IL131312A0 (pt)
OA (1) OA11147A (pt)
SK (1) SK110499A3 (pt)
WO (1) WO1998036821A1 (pt)
ZA (1) ZA981370B (pt)

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* Cited by examiner, † Cited by third party
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US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
AUPQ697300A0 (en) 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
ITRM20010070A1 (it) * 2001-02-12 2002-08-12 Ct Sviluppo E Applic Tita Nio Procedimento di evoluzione controllata di vini e relativo dispositivodi attuazione.
JP4413775B2 (ja) * 2002-06-07 2010-02-10 ピコセプ アクティーゼルスカブ 液体に含まれる生体成分の分離方法、分離システムおよび分離ユニット
CN100567597C (zh) * 2003-09-03 2009-12-09 史密尔·布克施班 一种用于生物分子快速结晶的方法和装置
WO2008089120A2 (en) * 2007-01-12 2008-07-24 Integrated Environmental Technologies, Ltd. Aqueous solution for managing microbes in oil and gas production and method for their production
US20080299624A1 (en) * 2007-06-01 2008-12-04 Edward Heslop Continuous fermentation apparatus and method
CA2749272A1 (en) * 2008-01-11 2009-07-16 Linde North America, Inc. Subcritical and supercritical fluid extraction of fat and drying residual solids for protein/carbohydrate content from food industry liquid or solid waste
US9475049B2 (en) 2009-07-31 2016-10-25 Invisible Sentinel, Inc. Analyte detection devices, multiplex and tabletop devices for detection of analyte, and uses thereof
US8012770B2 (en) 2009-07-31 2011-09-06 Invisible Sentinel, Inc. Device for detection of antigens and uses thereof
WO2011044574A1 (en) 2009-10-09 2011-04-14 Invisible Sentinel Device for detection of antigens and uses thereof
CN102824854A (zh) * 2011-06-15 2012-12-19 杜权 一种电泳装置及其应用
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
WO2013067509A1 (en) 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
US9658195B2 (en) * 2012-02-15 2017-05-23 Bio-Rad Laboratories, Inc. Electronic control of pH and ionic strength
CA2866379C (en) 2012-03-09 2020-10-13 Invisible Sentinel, Inc. Methods and compositions for detecting multiple analytes with a single signal
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
CN104307367B (zh) * 2014-10-27 2016-06-01 上海交通大学 一种往复式自由流等电聚焦电泳装置及方法
CN109260231B (zh) * 2017-07-18 2021-09-03 首都儿科研究所 一种蚯蚓中止咳祛痰抗炎抗微生物提取物的制备方法
CN112940915B (zh) * 2021-03-31 2025-07-04 四川工易生物科技有限公司 一种质粒提取装置及方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4465582A (en) * 1982-05-24 1984-08-14 Mcdonnell Douglas Corporation Continuous flow electrophoresis apparatus
GB8318754D0 (en) * 1983-07-11 1983-08-10 Fagerhol M K F Human proteins anti-sera test kits
GB8412207D0 (en) * 1984-05-12 1984-06-20 Wellcome Found Antigenic preparations
FR2568485B1 (fr) * 1984-08-06 1990-03-23 Rhone Poulenc Rech Appareil de fractionnement par electrophorese de solutions contenant des proteines, utilisable notamment pour le fractionnement du plasma humain
GB8706599D0 (en) * 1987-03-19 1987-04-23 Hoffmann La Roche Plasmodium falciparum merozoite antigen peptides
US4971616A (en) * 1987-04-08 1990-11-20 Glogowski Mark E Process for preparing organic compost from municipal refuse
US5160594A (en) * 1989-03-08 1992-11-03 Board Of Regents Of The University Of Texas System Apparatus and methods for isoelectric focusing of amphoteric substances incorporating ion selective membranes in electrode chambers
US5576026A (en) * 1994-09-30 1996-11-19 Charter; Edward A. Lumbricus product and method of making same

Also Published As

Publication number Publication date
CA2281867A1 (en) 1998-08-27
AU739328B2 (en) 2001-10-11
CN1245246C (zh) 2006-03-15
JP2001513013A (ja) 2001-08-28
CN1251538A (zh) 2000-04-26
DE69815069T2 (de) 2004-04-01
AP9901607A0 (en) 1999-09-30
DE69815069D1 (de) 2003-07-03
EP0963237B1 (en) 2003-05-28
KR20000071204A (ko) 2000-11-25
KR100493975B1 (ko) 2005-06-10
US6572751B1 (en) 2003-06-03
OA11147A (en) 2003-04-22
SK110499A3 (en) 2000-05-16
IL131312A0 (en) 2001-01-28
ZA981370B (en) 1998-09-07
WO1998036821A1 (en) 1998-08-27
AU6124498A (en) 1998-09-09
ATE241422T1 (de) 2003-06-15
EP0963237A1 (en) 1999-12-15
US20030206894A1 (en) 2003-11-06

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 5A , 6A , 7A E 8A ANUIDADES.