BR0117012A - Detection of spore-forming bacteria - Google Patents
Detection of spore-forming bacteriaInfo
- Publication number
- BR0117012A BR0117012A BR0117012-0A BR0117012A BR0117012A BR 0117012 A BR0117012 A BR 0117012A BR 0117012 A BR0117012 A BR 0117012A BR 0117012 A BR0117012 A BR 0117012A
- Authority
- BR
- Brazil
- Prior art keywords
- spore
- bacteria
- forming
- nucleic acid
- probe
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 7
- 238000001514 detection method Methods 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 239000000523 sample Substances 0.000 abstract 5
- 150000007523 nucleic acids Chemical group 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 241000193403 Clostridium Species 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 108091092356 cellular DNA Proteins 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000002853 nucleic acid probe Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000028070 sporulation Effects 0.000 abstract 1
- 230000009897 systematic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
"DETECçãO DE BACTéRIAS FORMADORAS DE ESPOROS". A presente invenção refere-se a métodos divulgados, sondas e seq³ências de ácido nucléico para a identificação sistemática de genes da esporulação em bactérias formadoras de esporos. Uma sonda compreende uma seq³ência de ácido nucléico capaz de formar um híbrido detectável com regiões altamente conservadas do gene spoOA de certas espécies de bactérias de Bacillus e de Clostridium de bactérias formadoras de esporos, a dita seq³ência de ácido nucléico sendo incapaz de formar um híbrido detectável com o material genético de bactérias que não formam esporos. A amplificação de uma parte do gene spoOA do DNA celular de uma bactéria formadora de esporos através da reação em cadeia da polimerase utilizando tal sonda, iniciador ou seq³ência de ácido nucléico como um membro de um conjunto de iniciadores resulta na obtenção de um produto de DNA detectável com 346-365 nucleotídeos de comprimento. Um método divulgado compreende a) a combinação de uma sonda com ponta ou marcação com uma amostra, b) a hibridização da sonda com o gene spoOA alvo das bactérias formadoras de esporos; e c) a detecção do produto hibridizado."DETECTION OF SPORE FORMING BACTERIA". The present invention relates to disclosed methods, probes and nucleic acid sequences for the systematic identification of sporulation genes in spore-forming bacteria. A probe comprises a nucleic acid sequence capable of forming a detectable hybrid with highly conserved spoOA gene regions of certain Bacillus and Clostridium bacteria species of spore-forming bacteria, said nucleic acid sequence being unable to form a detectable hybrid. with the genetic material of non-spore bacteria. Amplification of a portion of the spoOA gene from the cellular DNA of a spore-forming bacterium by polymerase chain reaction using such a nucleic acid probe, primer or sequence as a member of a primer set results in the obtaining of a DNA product. detectable 346-365 nucleotides in length. A disclosed method comprises a) combining a tipped or labeled probe with a sample, b) hybridizing the probe to the target spoOA gene of spore-forming bacteria; and c) detecting the hybridized product.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US2001/015793 WO2002092853A1 (en) | 2001-05-15 | 2001-05-15 | Detection of spore forming bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0117012A true BR0117012A (en) | 2004-08-03 |
Family
ID=21742576
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0117012-0A BR0117012A (en) | 2001-05-15 | 2001-05-15 | Detection of spore-forming bacteria |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1390527A1 (en) |
| CN (1) | CN1507496A (en) |
| BR (1) | BR0117012A (en) |
| CA (1) | CA2443441A1 (en) |
| MX (1) | MXPA03009460A (en) |
| NO (1) | NO20035082D0 (en) |
| WO (1) | WO2002092853A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102304559B (en) * | 2010-02-05 | 2013-03-20 | 山东出入境检验检疫局检验检疫技术中心 | Fluorescence quantitative polymerase chain reaction (PCR) method for detecting bacillus coagulans quickly |
| CA3069500C (en) | 2017-07-12 | 2024-06-04 | Ecolab Usa Inc. | A method for the rapid detection of bacterial spores in an industrial process |
| CN110869510A (en) * | 2017-07-12 | 2020-03-06 | 埃科莱布美国股份有限公司 | Rapid method for detecting bacterial spores |
| CN109706238B (en) | 2017-10-26 | 2023-04-07 | 珠海岐微生物科技有限公司 | Method for detecting and treating age-related macular degeneration |
| CN110592243A (en) * | 2019-09-24 | 2019-12-20 | 湖北广济药业股份有限公司 | Vitamin B2qPCR detection method for residual live bacteria and spores of medium-producing strain, and primers and probes used in method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0786013A1 (en) * | 1994-10-14 | 1997-07-30 | The University of Wales, Aberystwyth | Method of monitoring endospore-forming bacteria |
| US5928875A (en) * | 1998-05-27 | 1999-07-27 | Betzdearborn Inc. | Primers for the detection of spore forming bacteria |
| JP3121332B1 (en) * | 1999-11-10 | 2000-12-25 | 日本甜菜製糖株式会社 | Detection of sporulating Clostridium bacteria |
-
2001
- 2001-05-15 WO PCT/US2001/015793 patent/WO2002092853A1/en not_active Ceased
- 2001-05-15 CA CA002443441A patent/CA2443441A1/en not_active Abandoned
- 2001-05-15 MX MXPA03009460A patent/MXPA03009460A/en unknown
- 2001-05-15 EP EP01939059A patent/EP1390527A1/en not_active Withdrawn
- 2001-05-15 CN CNA018232612A patent/CN1507496A/en active Pending
- 2001-05-15 BR BR0117012-0A patent/BR0117012A/en not_active IP Right Cessation
-
2003
- 2003-11-14 NO NO20035082A patent/NO20035082D0/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2443441A1 (en) | 2002-11-21 |
| EP1390527A1 (en) | 2004-02-25 |
| CN1507496A (en) | 2004-06-23 |
| WO2002092853A1 (en) | 2002-11-21 |
| NO20035082D0 (en) | 2003-11-14 |
| MXPA03009460A (en) | 2004-04-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application fees: dismissal - article 86 of industrial property law |
Free format text: REFERENTE A 6A, 7A E 8A ANUIDADE(S). |
|
| B08K | Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87) |
Free format text: REFERENTE AO DESPACHO 8.6 DA RPI 2021 DE 29/09/2009. |