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BR0011249A - Methods and compositions for high molecular weight DNA isolation from natural samples - Google Patents

Methods and compositions for high molecular weight DNA isolation from natural samples

Info

Publication number
BR0011249A
BR0011249A BR0011249-6A BR0011249A BR0011249A BR 0011249 A BR0011249 A BR 0011249A BR 0011249 A BR0011249 A BR 0011249A BR 0011249 A BR0011249 A BR 0011249A
Authority
BR
Brazil
Prior art keywords
compositions
molecular weight
methods
high molecular
weight dna
Prior art date
Application number
BR0011249-6A
Other languages
Portuguese (pt)
Inventor
Iam Mcneil
Berkley A Lynch
Kara A Loiacono
Cho Lai Tiong
Charles A Minor
Marcia S Osburne
Trudy H Grossman
Paul R August
Original Assignee
Aventis Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Inc filed Critical Aventis Pharma Inc
Publication of BR0011249A publication Critical patent/BR0011249A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C12P17/165—Heterorings having nitrogen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"MéTODOS E COMPOSIçõES PARA ISOLAMENTO DE DNA DE ELEVADO PESO MOLECULAR A PARTIR DE AMOSTRAS NATURAIS". São divulgados métodos e composições para isolamento e propagação de DNA de elevado peso molecular. Grandes segmentos de DNA isolado de amostras obtidas diretamente de fontes naturais tal como o solo, amostras submarinas, água doce e salgada, ar e outras fontes podem ser usados para examinar agrupamentos genéticos inteiros e isolar produtos de expressão de organismos anteriormente desconhecidos e não caracterizados ou mesmo extintos. O isolamento e manipulação de grandes segmentos de DNA que não era possível anteriormente é ensinado aqui."METHODS AND COMPOSITIONS FOR INSULATION OF HIGH MOLECULAR WEIGHT DNA FROM NATURAL SAMPLES". Methods and compositions for isolating and propagating high molecular weight DNA are disclosed. Large segments of DNA isolated from samples obtained directly from natural sources such as soil, underwater samples, fresh and salt water, air and other sources can be used to examine entire genetic clusters and isolate expression products from previously unknown and uncharacterized organisms or even extinct. The isolation and manipulation of large segments of DNA that was not previously possible is taught here.

BR0011249-6A 1999-06-02 2000-06-01 Methods and compositions for high molecular weight DNA isolation from natural samples BR0011249A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13706599P 1999-06-02 1999-06-02
US19160100P 2000-03-23 2000-03-23
PCT/US2000/015306 WO2000073508A1 (en) 1999-06-02 2000-06-01 Methods and compositions for isolating high molecular weight dna from natural samples

Publications (1)

Publication Number Publication Date
BR0011249A true BR0011249A (en) 2002-10-22

Family

ID=26834891

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0011249-6A BR0011249A (en) 1999-06-02 2000-06-01 Methods and compositions for high molecular weight DNA isolation from natural samples

Country Status (11)

Country Link
EP (1) EP1208229A4 (en)
JP (1) JP2003520024A (en)
KR (1) KR20020034087A (en)
AU (1) AU778943B2 (en)
BR (1) BR0011249A (en)
CA (1) CA2375873A1 (en)
IL (1) IL146728A0 (en)
MX (1) MXPA01012345A (en)
NO (1) NO20015730L (en)
NZ (1) NZ515641A (en)
WO (1) WO2000073508A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3100739A1 (en) * 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0338591A3 (en) * 1988-04-21 1991-09-04 Microprobe Corporation Nucleic acid extraction method
IT1240870B (en) * 1990-02-14 1993-12-17 Talent PROCEDURE FOR THE EXTRACTION AND PURIFICATION OF HUMAN GENOMIC DNA
WO1993010230A1 (en) * 1991-11-20 1993-05-27 Trustees Of Dartmouth College Ligand-stimulated gene expression
WO1993025711A1 (en) * 1992-06-12 1993-12-23 Gen-Probe Incorporated Preparation of nucleic acid from blood
CA2219136A1 (en) * 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways

Also Published As

Publication number Publication date
AU5178700A (en) 2000-12-18
EP1208229A4 (en) 2005-06-22
JP2003520024A (en) 2003-07-02
IL146728A0 (en) 2002-07-25
KR20020034087A (en) 2002-05-08
NO20015730L (en) 2002-02-01
MXPA01012345A (en) 2002-07-22
CA2375873A1 (en) 2000-12-07
EP1208229A1 (en) 2002-05-29
NZ515641A (en) 2003-11-28
NO20015730D0 (en) 2001-11-23
WO2000073508A1 (en) 2000-12-07
AU778943B2 (en) 2004-12-23

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7O, 8O E 9O ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 DA RPI 2008 DE 30/06/2009.