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BE1028737B1 - Essai de traduction de codons sensibles à la modification de l'ARNt comprenant un système rapporteur basé sur la luminescence - Google Patents

Essai de traduction de codons sensibles à la modification de l'ARNt comprenant un système rapporteur basé sur la luminescence

Info

Publication number
BE1028737B1
BE1028737B1 BE20215237A BE202105237A BE1028737B1 BE 1028737 B1 BE1028737 B1 BE 1028737B1 BE 20215237 A BE20215237 A BE 20215237A BE 202105237 A BE202105237 A BE 202105237A BE 1028737 B1 BE1028737 B1 BE 1028737B1
Authority
BE
Belgium
Prior art keywords
luminescence
based reporter
reporter system
trna
modification including
Prior art date
Application number
BE20215237A
Other languages
English (en)
Inventor
Pierre Close
Alain Chariot
Francesca Rapino
Original Assignee
Univ Liege
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Liege filed Critical Univ Liege
Priority to BE20215237A priority Critical patent/BE1028737B1/fr
Priority to PCT/EP2022/054902 priority patent/WO2022207206A1/fr
Priority to EP22710024.5A priority patent/EP4314299A1/fr
Priority to US18/284,924 priority patent/US20240376555A1/en
Application granted granted Critical
Publication of BE1028737B1 publication Critical patent/BE1028737B1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Les inventeurs ont découvert qu'un essai utilisant des lignées cellulaires humaines transformées de manière stable comprenant des répétitions de codons sensibles aux modifications de l'ARNt en amont d'un premier gène rapporteur basé sur la luminescence et des répétitions de leurs codons synonymes en amont d'un second gène rapporteur basé sur la luminescence peut être utile pour identifier rapidement les modulateurs des enzymes modifiant l'ARNt.
BE20215237A 2021-03-29 2021-03-29 Essai de traduction de codons sensibles à la modification de l'ARNt comprenant un système rapporteur basé sur la luminescence BE1028737B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
BE20215237A BE1028737B1 (fr) 2021-03-29 2021-03-29 Essai de traduction de codons sensibles à la modification de l'ARNt comprenant un système rapporteur basé sur la luminescence
PCT/EP2022/054902 WO2022207206A1 (fr) 2021-03-29 2022-02-27 Essai de traduction de codon sensible à la modification d'arnt comprenant un système rapporteur basé sur la luminescence
EP22710024.5A EP4314299A1 (fr) 2021-03-29 2022-02-27 Essai de traduction de codon sensible à la modification d'arnt comprenant un système rapporteur basé sur la luminescence
US18/284,924 US20240376555A1 (en) 2021-03-29 2022-02-27 Tnra modification-sensitive codon translation assay comprising luminescence-based reporter system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BE20215237A BE1028737B1 (fr) 2021-03-29 2021-03-29 Essai de traduction de codons sensibles à la modification de l'ARNt comprenant un système rapporteur basé sur la luminescence

Publications (1)

Publication Number Publication Date
BE1028737B1 true BE1028737B1 (fr) 2022-05-19

Family

ID=75441689

Family Applications (1)

Application Number Title Priority Date Filing Date
BE20215237A BE1028737B1 (fr) 2021-03-29 2021-03-29 Essai de traduction de codons sensibles à la modification de l'ARNt comprenant un système rapporteur basé sur la luminescence

Country Status (4)

Country Link
US (1) US20240376555A1 (fr)
EP (1) EP4314299A1 (fr)
BE (1) BE1028737B1 (fr)
WO (1) WO2022207206A1 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018013720A1 (fr) * 2016-07-12 2018-01-18 Washington University Incorporation d'étiquettes de séquence poly-lysine codées par polya interne et leurs variations pour la régulation accordable de la synthèse de protéines dans des cellules bactériennes et eucaryotes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2692159C (fr) 2007-06-22 2016-09-13 Barry S. Cooperman Marquage fluorescent d'arn de transfert et etude d'une synthese de proteine
WO2017011766A1 (fr) 2015-07-16 2017-01-19 Cornell University Procédés d'amélioration de la capacité de traduction de molécules d'arn, traitements, et kits
US10907165B2 (en) 2016-10-31 2021-02-02 Cornell University Methods of enhancing translation ability and stability of RNA molecules, treatments, and kits

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018013720A1 (fr) * 2016-07-12 2018-01-18 Washington University Incorporation d'étiquettes de séquence poly-lysine codées par polya interne et leurs variations pour la régulation accordable de la synthèse de protéines dans des cellules bactériennes et eucaryotes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
NANDINI MANICKAM ET AL: "Effects of tRNA modification on translational accuracy depend on intrinsic codon–anticodon strength", NUCLEIC ACIDS RESEARCH, vol. 44, no. 4, 29 February 2016 (2016-02-29), GB, pages 1871 - 1881, XP055527065, ISSN: 0305-1048, DOI: 10.1093/nar/gkv1506 *
RAPINO FRANCESCA ET AL: "Codon-specific translation reprogramming promotes resistance to targeted therapy", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 558, no. 7711, 20 June 2018 (2018-06-20), pages 605 - 609, XP037617145, ISSN: 0028-0836, [retrieved on 20180620], DOI: 10.1038/S41586-018-0243-7 *
RAPINO FRANCESCA ET AL: "Wobble tRNA modification and hydrophilic amino acid patterns dictate protein fate", NATURE COMMUNICATIONS, vol. 12, no. 1, 15 April 2021 (2021-04-15), XP009531474 *
ROSU ADELINE ET AL: "Loss of tRNA-modifying enzyme Elp3 activates a p53-dependent antitumor checkpoint in hematopoiesis", JOURNAL OF EXPERIMENTAL MEDICINE, vol. 218, no. 3, 1 March 2021 (2021-03-01), US, XP055863396, ISSN: 0022-1007, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849823/pdf/JEM_20200662.pdf> DOI: 10.1084/jem.20200662 *
SYLVAIN DELAUNAY ET AL: "Elp3 links tRNA modification to IRES-dependent translation of LEF1 to sustain metastasis in breast cancer", JOURNAL OF EXPERIMENTAL MEDICINE, vol. 213, no. 11, 17 October 2016 (2016-10-17), US, pages 2503 - 2523, XP055756388, ISSN: 0022-1007, DOI: 10.1084/jem.20160397 *

Also Published As

Publication number Publication date
EP4314299A1 (fr) 2024-02-07
US20240376555A1 (en) 2024-11-14
WO2022207206A1 (fr) 2022-10-06

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FG Patent granted

Effective date: 20220519