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AU9745298A - Method for synthesising and amplifying nucleic acids - Google Patents

Method for synthesising and amplifying nucleic acids

Info

Publication number
AU9745298A
AU9745298A AU97452/98A AU9745298A AU9745298A AU 9745298 A AU9745298 A AU 9745298A AU 97452/98 A AU97452/98 A AU 97452/98A AU 9745298 A AU9745298 A AU 9745298A AU 9745298 A AU9745298 A AU 9745298A
Authority
AU
Australia
Prior art keywords
nucleic acid
synthesising
copied
nucleic acids
initiator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU97452/98A
Inventor
Ralf Ehricht
Thomas Ellinger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INSTITUT fur MOLEKULARE BIOTECHNOLOGIE EV
Original Assignee
Institut fuer Physikalische Hochtechnologie eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut fuer Physikalische Hochtechnologie eV filed Critical Institut fuer Physikalische Hochtechnologie eV
Publication of AU9745298A publication Critical patent/AU9745298A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

The invention relates to a method for synthesising and amplifying nucleic acids by means of an enzyme-catalysed reaction wherein a nucleic acid is copied on the basis of a single-stranded initiator nucleic acid without said initiator nucleic acid hybridising with the nucleic acid being copied. According to the inventive method, a linear nucleic acid is copied on the basis of an initiator nucleic acid with a single-stranded area at least at the 3' end, through the activity of a polymerase. The initiator nucleic acid does not need to be homologous with the nucleic acid being copied.
AU97452/98A 1997-09-22 1998-09-21 Method for synthesising and amplifying nucleic acids Abandoned AU9745298A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19741714A DE19741714C2 (en) 1997-09-22 1997-09-22 Process for the synthesis and amplification of nucleic acids
DE19741714 1997-09-22
PCT/EP1998/006006 WO1999015698A2 (en) 1997-09-22 1998-09-21 Method for synthesising and amplifying nucleic acids

Publications (1)

Publication Number Publication Date
AU9745298A true AU9745298A (en) 1999-04-12

Family

ID=7843182

Family Applications (1)

Application Number Title Priority Date Filing Date
AU97452/98A Abandoned AU9745298A (en) 1997-09-22 1998-09-21 Method for synthesising and amplifying nucleic acids

Country Status (6)

Country Link
EP (1) EP1015640B1 (en)
JP (1) JP2001517458A (en)
AT (1) ATE476519T1 (en)
AU (1) AU9745298A (en)
DE (2) DE19741714C2 (en)
WO (1) WO1999015698A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6994963B1 (en) 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US20040009356A1 (en) 2002-05-03 2004-01-15 Medwick Paul A. Substrate having thermal management coating for an insulating glass unit
JP2004357502A (en) * 2003-05-30 2004-12-24 Olympus Corp Method for processing information using nucleic acid molecule
DE102004029051C5 (en) * 2004-06-11 2011-09-15 Schunk Gmbh & Co. Kg Spann- Und Greiftechnik Fastening device for attaching a top jaw to a movably mounted base jaw of a linear or centric gripper and linear or centric gripper

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2678639B1 (en) * 1991-07-03 1993-09-17 Rhone Poulenc Rorer Sa PROCESS FOR CLONING NUCLEIC ACIDS.
DE4428651C1 (en) * 1994-08-12 1996-02-29 Inst Molekulare Biotechnologie Process for the production and amplification of nucleic acids
IL111857A (en) * 1994-12-02 1999-09-22 Intelligene Ltd Detection of nucleic acid sequences
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
EP0914462A4 (en) * 1996-03-18 2002-05-22 Molecular Biology Resources Target nucleic acid sequence amplification

Also Published As

Publication number Publication date
DE59814461D1 (en) 2010-09-16
WO1999015698A3 (en) 1999-06-10
WO1999015698A2 (en) 1999-04-01
DE19741714C2 (en) 2002-03-21
EP1015640A2 (en) 2000-07-05
ATE476519T1 (en) 2010-08-15
DE19741714A1 (en) 1999-03-25
JP2001517458A (en) 2001-10-09
EP1015640B1 (en) 2010-08-04

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase