AU2018219977A1

AU2018219977A1 - K-ras mutations and anti-EGFr antibody therapy field

Info

Publication number: AU2018219977A1
Application number: AU2018219977A
Authority: AU
Inventors: Daniel Freeman; Todd Juan; Robert Radinsky
Original assignee: Amgen Inc
Current assignee: Amgen Inc
Priority date: 2007-03-13
Filing date: 2018-08-20
Publication date: 2018-09-06
Anticipated expiration: 2028-03-11
Also published as: AU2016250348A1; AU2018219977B2

Abstract

The present application relates to K-ras mutations, to polynucleotides encoding mutant K-ras polypeptides, and to methods of identifying K-ras mutations. The present application also relates to methods of diagnosing cancer; and methods and kits for predicting usefulness of anti-EGFr specific binding agents in the treatment of tumors.

Description

BACKGROUND [0003] Certain applications of monoclonal antibodies in cancer therapy rely on the ability of the antibody to specifically deliver to the cancerous tissues cytotoxic effector functions such as immune-enhancing isotypes, toxins or drugs. An alternative approach is to utilize monoclonal antibodies to directly affect the survival of tumor cells by depriving them of essential extracellular proliferation signals, such as those mediated by growth factors through their cell receptors. One of the attractive targets in this approach is the epidermal growth factor receptor (EGFr), which binds EGF and transforming growth factor a (TGFa) (see, e.g., Ullrich et al., Cell 61:203-212, 1990; Baselga et al., Pharmacol. Ther. 64: 127-154, 1994; Mendelsohn et al., in Biologic Therapy of Cancer 607-623, Philadelphia: J.B. Lippincott Co., 1995; Fan et al., Curr. Opin. Oncol. 10: 67-73, 1998). Binding of EGF orTGFato EGFr, a 170 kDa transmembrane cell surface glycoprotein, triggers a cascade of cellular biochemical events, including EGFr autophosphorylation and internalization, which culminates in cell proliferation (see, e.g., Ullrich et al., Cell 61:203-212, 1990).

[0004] Several observations implicate EGFr in supporting development and progression of human solid tumors. EGFr has been demonstrated to be overexpressed on many types of human solid tumors (see, e.g., Mendelsohn Cancer Cells 7:359 (1989),

Mendelsohn Cancer Biology 1:339-344 (1990), Modjtahedi and Dean Int’l J. Oncology 4:277296 (1994)). For example, EGF-r overexpression has been observed in certain

2018219977 20 Aug 2018 lung, breast, colon, gastric, brain, bladder, head and neck, ovarian, and prostate carcinomas (see, e.g., Modjtahedl and Dean Int’l J. Oncology4:277296 (1994)). The increase in receptor levels has been reported to be associated with a poor clinical prognosis (see, e.g., Baselga et al. Pharmacol, Ther. 64: 127-154, 1994; Mendelsohn et al., Biologic Therapy of Cancer pp. 607-623, Philadelphia: J.B. Lippincott Co., 1995; Modjtahedi etal., Inti. J. of Oncology 4:277-296, 1994; Gullick, Br. Medical Bulletin, 47:87-98, 1991; Salomon etal., Crit. Rev. Oncol. Hematoi. 19: 183-232, 1995). Both epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-a) have been demonstrated to bind to EGF-r and to lead to cellular proliferation and tumor growth. In many cases, increased surface EGFr expression was accompanied by production of TGFa or EGF by tumor cells, suggesting the involvement of an autocrine growth control in the progression of those tumors (see, e.g., Baselga et al. Pharmacol. Ther. 64: 127-154, 1994; Mendelsohn et al., Biologic Therapy of Cancer pp. 607-623, Philadelphia: J.B. Lippincott Co., 1995; Modjtahedi et al.. Inti. J. of Oncology 4:277-296, 1994; Salomon et al., Crit. Rev. Oncol. Hematoi. 19: 183-232, 1995).

[005] Thus, certain groups have proposed that antibodies against EGF, TGF-α, and EGF-r may be useful in the therapy of tumors expressing or overexpressing EGF-r (see, e.g., Mendelsohn Cancer Cells 7:359 (1989), Mendelsohn Cancer Biology 1:339-344 (1990), Modjtahedi and Dean Int’l J. Oncology 4:277-296 (1994), Tosi et al. Int’l J. Cancer62:643-650 (1995)). Indeed, it has been demonstrated that anti-EGF-r antibodies blocking EGF and TGF-α binding to the receptor appear to inhibit tumor cell proliferation. At the same time, however, anti-EGF-r antibodies have not appeared to inhibit EGF and TGF-α independent cell growth (Modjtahedi and Dean Int’l J. Oncology 4:277-296 (1994)).

[006] Monoclonal antibodies specific to the human EGFr, capable of neutralizing EGF and TGFa binding to tumor cells and of inhibiting ligandmediated cell proliferation in vitro, have been generated from mice and rats (see, e.g., Baselga etal., Pharmacol. Ther. 64: 127-154, 1994; Mendelsohn et al., in Biologic Therapy of Cancer pp. 607-623, Philadelphia: J.B. Lippincott

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Co., 1995; Fan et al., Curr. Opin. Oncol. 10: 67-73, 1998; Modjtahedi et al., Inti. J. Oncology 4: 277-296, 1994). Some of those antibodies, such as the mouse 108, 225 (see, e.g., Aboud-Pirak et al., J. Natl. Cancer Inst. 80: 16051611, 1988) and 528 (see, e.g., Baselga et al., Pharmacol. Ther. 64: 127-154, 1994; Mendelsohn et al., in Biologic Therapy of Cancer pp. 607-623, Philadelphia: J.B. Lippincott Co., 1995) or the rat ICR16, ICR62 and ICR64 (see, e.g., Modjtajedi et al., Inti. J. Oncology 4: 277-296, 1994; Modjtahedi et al., Br. J. Cancer67:247-253, 1993; Modjtahedi et al., Br. J. Cancer67: 254261, 1993) monoclonal antibodies, were evaluated extensively for their ability to affect tumor growth in xenograft mouse models. Most of the anti-EGFr monoclonal antibodies were efficacious in preventing tumor formation in athymic mice when administered together with the human tumor cells (Baselga et al. Pharmacol. Ther. 64: 127-154, 1994; Modjtahedi et al., Br. J. Cancer 67: 254-261, 1993 ). When injected into mice bearing established human tumor xenografts, the mouse monoclonal antibodies 225 and 528 caused partial tumor regression and required the co-administration of chemotherapeutic agents, such as doxorubicin or cisplatin, for eradication of the tumors (Baselga et al. Pharmacol. Ther. 64: 127-154, 1994; Mendelsohn et al., in Biologic Therapy of Cancer pp. 607-623, Philadelphia: J.B. Lippincott Co., 1995; Fan et al., Cancer Res. 53: 4637-4642, 1993; Baselga et al., J. Natl. Cancer Inst. 85: 1327-1333, 1993), A chimeric version ofthe 225 monoclonal antibody (C225), in which the mouse antibody variable regions are linked to human constant regions, exhibited an improved in vivo antitumor activity but only at high doses (see, e g., Goldstein et al., Clinical Cancer Res. 1: 1311-1318, 1995; Prewett et al., J. Immunother. Emphasis Tumor Immunol. 19: 419-427, 1996). The rat ICR16, ICR62, and ICR64 antibodies caused regression of established tumors but not their complete eradication (Modjtahedi et al., Br. J. Cancer67: 254-261, 1993). These results established EGFr as a promising target for antibody therapy against EGFr-expressing solid tumors and led to human clinical trials with the C225 monoclonal antibody in multiple human solid cancers (see, e.g., Baselga et al. Pharmacol. Ther. 64: 127-154, 1994; Mendelsohn et al., Biologic Therapy of

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Cancer pp. 607-623, Philadelphia: J.B. Lippincott Co., 1995; Modjtahedi et al., Inti. J. of Oncology 4:277-296, 1994).

[007] Certain advances in the biological arts made it possible to produce a fully human anti-EGFr antibody. Using mice transgenic for human immunoglobulin genes (Xenomouse™ technology, Abgenix, Inc.), human antibodies specific for human EGFr were developed (see, e.g., Mendez, Nature Genetics, 15: 146-156, 1997; Jakobovits, Adv. Drug Deliv. Rev., 31(12): 33-42,1998; Jakobovits, Expert Opin. Invest. Drugs, 7(4): 607-614,1998; Yang et al., Crit. Rev. Oncol. Hematol. 38(1):17-23, 2001; WO98/24893; WO 98/50433). One such antibody, panitumumab, a human lgG2 monoclonal antibody with an affinity of 5 x 10'¹¹ M for human EGFr, has been shown to block binding of EGF to the EGFr, to block receptor signaling, and to inhibit tumor cell activation and proliferation in vitro (see, e.g., WO98/50433; U.S. Patent No. 6,235,883). Studies in athymic mice have demonstrated that panitumumab also has in vivo activity, not only preventing the formation of human epidermoid carcinoma A431 xenografts in athymic mice, but also eradicating already-established large A431 tumor xenografts (see, e.g., Yang et al._r Crit. Rev. Oncol. Hematol. 38(1):17-23, 2001; Yang et al., Cancer Res. 59(6):1236-43, 1999). Panitumumab has been considered for the treatment of renal carcinoma, colorectal adenocarcinoma, prostate cancer, and non small cell squamous lung carcinoma, among other cancers (see, e.g., U.S. Patent Publication No. 2004/0033543), and clinical trials are underway with that antibody. Panitumumab has been approved by the Food & Drug Administration to treat patients with metastatic colorectal cancer.

[008] Activation of EGFr triggers at least two signalling pathways. In certain cell types, activation of EGFr prevents apoptosis by stimulation of phosphatidylinositol 3-kinase (“PI3K). PI3K activation triggers a molecular cascade leading to the downregulation ofthe central pathways controlling programmed cell death (Yao, R., Science 267:2003-2006, 1995). In certain cell types, activation of EGFr initiates the MAPK cascade through Ras/Raf. SUMMARY [009] In certain embodiments, a method of predicting whether a patient will be nonresponsive to treatment with a specific binding agent to an

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EGFr polypeptide is provided. In certain embodiments, the method comprises determining the presence or absence of a K-ras mutation in a tumor of the patient, wherein the K-ras mutation is in codon 12 or codon 13 or codon 20. In certain embodiments, if a K-ras mutation is present, the patient is predicted to be nonresponsive to treatment with a specific binding agent to an EGFr polypeptide.

[010] In certain embodiments, a method of predicting whether a tumor will be nonresponsive to treatment with a specific binding agent to an EGFr polypeptide is provided. In certain embodiments, the method comprises determining the presence or absence of a K-ras mutation in a sample of said tumor, wherein the K-ras mutation is in codon 12 or codon 13 or codon 20. In certain embodiments, the presence of the K-ras mutation indicates that the tumor will be nonresponsive to treatment with a specific binding agent to an EGFr polypeptide.

BRIEF DESCRIPTION OF THE FIGURES [011 ] Figures 1A to 11 show the cDNA and amino acid sequences for wild-type K-ras (SEQ ID NOs: 1 and 2), G12S mutant K-ras (SEQ ID NOs: 3 and 4), G12V mutant K-ras (SEQ ID NOs: 5 and 6), G12D mutant K-ras (SEQ ID NOs: 7 and 8), G12A mutant K-ras (SEQ ID NOs: 9 and 10), G12C mutant K-ras (SEQ ID NOs: 11 and 12), G13A mutant K-ras (SEQ ID NOs: 13 and 14), G13D mutant K-ras (SEQ ID NOs: 15 and 16), and T20M mutant Kras (SEQ ID NOs: 17 and 18).

DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS [012] All references cited herein, including patents, patent applications, papers, textbooks, and the like, and the references cited therein, to the extent that they are not already, are hereby incorporated herein by reference in their entirety. In the event that one or more of the documents incorporated by reference defines a term that contradicts that term’s definition in this application, this application controls. The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described.

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Definitions [013] Unless otherwise defined, scientific and technical terms used in connection with the present invention shall have the meanings that are commonly understood by those of ordinary skill in the art. Further, unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular.

[014] Generally, nomenclatures utilized in connection with, and techniques of, cell and tissue culture, molecular biology, and protein and oligo- or polynucleotide chemistry and hybridization described herein are those well known and commonly used in the art. Standard techniques are used for recombinant DNA, oligonucleotide synthesis, and tissue culture and transformation (e.g., electroporation, lipofection). Enzymatic reactions and purification techniques are performed according to the manufacturer's specifications or as commonly accomplished in the art or as described herein. The foregoing techniques and procedures are generally performed according to conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification. See e.g., Sambrook et al. Molecular Cloning: A Laboratory Manual (2d ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989)), which is incorporated herein by reference. The nomenclatures utilized in connection with, and the laboratory procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those well known and commonly used in the art. Standard techniques are used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients.

[015] In this application, the use of or means and/or” unless stated otherwise. In the context of a multiple dependent claim, the use of “or” refers back to more than one preceding independent or dependent claim in the alternative only. Furthermore, the use ofthe term including, as well as other forms, such as includes” and “included”, is not limiting. Also, terms such as element or component” encompass both elements and

2018219977 20 Aug 2018 components comprising one unit and elements and components that comprise more than one subunit unless specifically stated otherwise.

[016] As utilized in accordance with the present disclosure, the following terms, unless otherwise indicated, shall be understood to have the following meanings:

[017] The terms isolated polynucleotide and isolated nucleic acid” are used interchangeably, and as used herein shall mean a polynucleotide of genomic, cDNA, or synthetic origin or some combination thereof, which by virtue of its origin (1) is not associated with all or a portion of a polynucleotide in which the isolated polynucleotide is found in nature, (2) is operably linked to a polynucleotide which it is not linked to in nature, or (3) does not occur in nature as part of a larger sequence.

[018] The terms isolated protein and “isolated polypeptide are used interchangeably, and as referred to herein mean a protein of cDNA, recombinant RNA, or synthetic origin, or some combination thereof, which by virtue of its origin, or source of derivation, (1) is not associated with proteins found in nature, (2) is free of other proteins from the same source, e.g. free of murine proteins, (3) is expressed by a cell from a different species, or (4) does not occur in nature.

[019] The terms polypeptide and “protein” are used interchangeably and are used herein as a generic term to refer to native protein, fragments, peptides, or analogs of a polypeptide sequence. Hence, native protein, fragments, and analogs are species of the polypeptide genus.

[020] The terminology “X#Y” in the context of a mutation in a polypeptide sequence is art-recognized, where indicates the location of the mutation in terms ofthe amino acid number ofthe polypeptide, “X” indicates the amino acid found at that position in the wild-type amino acid sequence, and Ύ” indicates the mutant amino acid at that position. For example, the notation “G12S” with reference to the K-ras polypeptide indicates that there is a glycine at amino acid number 12 ofthe wild-type Kras sequence, and that glycine is replaced with a serine in the mutant K-ras sequence.

2018219977 20 Aug 2018 [021] The terms “mutant K-ras polypeptide” and “mutant K-ras protein” are used interchangeably, and refer to a K-ras polypeptide comprising at least one K-ras mutation selected from G12S, G12V, G12D, G12A, G12C, G13A, G13D, and T20M. Certain exemplary mutant K-ras polypeptides include, but are not limited to, allelic variants, splice variants, derivative variants, substitution variants, deletion variants, and/or insertion variants, fusion polypeptides, orthologs, and interspecies homologs. In certain embodiments, a mutant K-ras polypeptide includes additional residues at the C- or N-terminus, such as, but not limited to, leader sequence residues, targeting residues, amino terminal methionine residues, lysine residues, tag residues and/or fusion protein residues.

[022] The term naturally-occurring as used herein as applied to an object refers to the fact that an object can be found in nature. For example, a polypeptide or polynucleotide sequence that is present in an organism (including viruses) that can be isolated from a source in nature and which has not been intentionally modified by man in the laboratory or otherwise is naturally-occurring.

[023] The term operably linked as used herein refers to the positioning of components such that they are in a relationship permitting them to function in their intended manner. A control sequence operably linked to a coding sequence is ligated in such a way that expression of the coding sequence is achieved under conditions compatible with the control sequences.

[024] The term control sequence as used herein refers to polynucleotide sequences which are necessary to effect the expression and processing of coding sequences to which they are ligated. The nature of such control sequences differs depending upon the host organism; in prokaryotes, such control sequences generally include promoter, ribosomal binding site, and transcription termination sequences; in eukaryotes, generally, such control sequences include promoters and transcription termination sequences. The term control sequences is intended to include, at a minimum, all components whose presence is essential for expression and processing, and can also include additional components whose presence

2018219977 20 Aug 2018 is advantageous, for example, leader sequences and fusion partner sequences.

[025] The term polynucleotide as referred to herein means a polymeric form of nucleotides of at least 10 bases in length, either ribonucleotides or deoxynucleotides or a modified form of either type of nucleotide. The term includes single and double stranded forms of DNA.

[026] The term oligonucleotide referred to herein includes naturally occurring and modified nucleotides linked together by naturally occurring, and non-naturally occurring oligonucleotide linkages. Oligonucleotides are a polynucleotide subset generally comprising a length of 200 bases or fewer. Preferably oligonucleotides are 10 to 60 bases in length and most preferably 12, 13, 14, 15, 16, 17, 18, 19, or 20 to 40 bases in length. Oligonucleotides are usually single stranded, e.g. for probes, although oligonucleotides may be double stranded, e g. for use in the construction of a gene mutant. Oligonucleotides of the invention can be either sense or antisense oligonucleotides.

[027] The terms “mutant K-ras polynucleotide”, “mutant K-ras oligonucleotide, and “mutant K-ras nucleic acid” are used interchangeably, and refer to a polynucleotide encoding a K-ras polypeptide comprising at least one K-ras mutation selected from G12S, G12V, G12D, G12A, G12C, G13A, G13D, and T20M.

[028] The term naturally occurring nucleotides referred to herein includes deoxyribonucleotides and ribonucleotides. The term modified nucleotides referred to herein includes nucleotides with modified or substituted sugar groups and the like. The term oligonucleotide linkages referred to herein includes oligonucleotide linkages such as phosphorothioate, phosphorodithioate, phosphoroselenoate, phosphorodiselenoate, phosphoroanilothioate, phoshoraniladate, phosphoroamidate, and the like. See e.g., LaPlanche et al. Nucl. Acids Res. 14:9081 (1986); Stec et al. J. Am. Chem. Soc. 106:6077 (1984); Stein et al. Nucl. Acids Res. 16:3209 (1988); Zon et al. Anti-Cancer Drug Design 6:539 (1991); Zon et al. Oligonucleotides and Analogues: A Practical Approach, pp. 87-108 (F. Eckstein, Ed., Oxford University Press, Oxford England (1991));

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Stec et al. U.S. Patent No. 5,151,510; Uhlmann and Peyman Chemical

Reviews 90:543 (1990), the disclosures of which are hereby incorporated by reference. An oligonucleotide can include a label for detection, if desired.

[029] The term selectively hybridize referred to herein means to detectably and specifically bind. Polynucleotides, oligonucleotides, and fragments thereof selectively hybridize to nucleic acid strands under hybridization and wash conditions that minimize appreciable amounts of detectable binding to nonspecific nucleic acids. High stringency conditions can be used to achieve selective hybridization conditions as known in the art and discussed herein. Generally, the nucleic acid sequence homology between polynucleotides, oligonucleotides, and fragments and a nucleic acid sequence of interest will be at least 80%, and more typically with preferably increasing homologies of at least 85%, 90%, 95%, 96%, 97%, 98%, 99%, and 100%. Two amino acid sequences are homologous if there is a partial or complete identity between their sequences. For example, 85% homology means that 85% of the amino acids are identical when the two sequences are aligned for maximum matching. Gaps (in either of the two sequences being matched) are allowed in maximizing matching; gap lengths of 5 or less are preferred with 2 or less being more preferred. Alternatively and preferably, two protein sequences (or polypeptide sequences derived from them of at least 30 amino acids in length) are homologous, as this term is used herein, if they have an alignment score of more than 5 (in standard deviation units) using the program ALIGN with the mutation data matrix and a gap penalty of 6 or greater. See Dayhoff, M. 0., in Atlas of Protein Sequence and Structure, pp. 101-110 (Volume 5, National Biomedical Research Foundation (1972)) and Supplement 2 to that volume, pp. 1-10. The two sequences or parts thereof are more preferably homologous if their amino acids are greater than or equal to 50% identical when optimally aligned using the ALIGN program. The term corresponds to is used herein to mean that a polynucleotide sequence is homologous (i.e., is identical, not strictly evolutlonarily related) to all or a portion of a reference polynucleotide sequence, or that a polypeptide sequence is identical to a reference polypeptide sequence. In contradistinction, the term complementary to is used herein to mean that the

2018219977 20 Aug 2018 complementary sequence is homologous to all or a portion of a reference polynucleotide sequence. For illustration, the nucleotide sequence TATAC corresponds to a reference sequence TATAC and is complementary to a reference sequence GTATA.

[030] The following terms are used to describe the sequence relationships between two or more polynucleotide or amino acid sequences: reference sequence, comparison window, sequence identity, percentage of sequence identity, and substantial identity. A reference sequence is a defined sequence used as a basis for a sequence comparison; a reference sequence may be a subset of a larger sequence, for example, as a segment of a full-length cDNA or gene sequence given in a sequence listing or may comprise a complete cDNA or gene sequence. Generally, a reference sequence is at least 18 nucleotides or 6 amino acids in length, or at least 24 nucleotides or 8 amino acids in length, or at least 48 nucleotides or 16 amino acids in length. Since two polynucleotides or amino acid sequences may each (1) comprise a sequence (i.e., a portion of the complete polynucleotide or amino acid sequence) that is similar between the two molecules, and (2) may further comprise a sequence that is divergent between the two polynucleotides or amino acid sequences, sequence comparisons between two (or more) molecules are typically performed by comparing sequences of the two molecules over a comparison window to identify and compare local regions of sequence similarity. A comparison window, as used herein, refers to a conceptual segment of at least 18 contiguous nucleotide positions or 6 amino acids wherein a polynucleotide sequence or amino acid sequence may be compared to a reference sequence of at least 18 contiguous nucleotides or 6 amino acid sequences and wherein the portion of the polynucleotide sequence in the comparison window may comprise additions, deletions, substitutions, and the like (i.e., gaps) of 20 percent or less as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. Optimal alignment of sequences for aligning a comparison window may be conducted by the local homology algorithm of Smith and Waterman Adv. Appl. Math. 2:482 (1981), by the homology alignment

2018219977 20 Aug 2018 algorithm of Needleman and Wunsch J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson and Lipman Proc. Natl. Acad. Sci. (U.S.A.) 85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package Release 7.0, (Genetics Computer Group, 575 Science Dr., Madison, Wis.), Geneworks, or MacVector software packages), or by inspection, and the best alignment (i.e., resulting in the highest percentage of homology over the comparison window) generated by the various methods is selected.

[031] The term sequence identity means that two polynucleotide or amino acid sequences are identical (i.e., on a nucleotide-by-nucleotide or residue-by-residue basis) over the comparison window. The term percentage of sequence identity is calculated by comparing two optimally aligned sequences over the window of comparison, determining the number of positions at which the identical nucleic acid base (e.g., A, T, C, G, U, or I) or residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the comparison window (i.e., the window size), and multiplying the result by 100 to yield the percentage of sequence identity. The terms substantial identity as used herein denotes a characteristic of a polynucleotide or amino acid sequence, wherein the polynucleotide or amino acid comprises a sequence that has at least 85 percent sequence identity, preferably at least 90 to 95 percent sequence identity, more usually at least 96, 97, 98, or 99 percent sequence identity as compared to a reference sequence over a comparison window of at least 18 nucleotide (6 amino acid) positions, frequently over a window of at least 24-48 nucleotide (8-16 amino acid) positions, wherein the percentage of sequence identity is calculated by comparing the reference sequence to the sequence which may include deletions or additions which total 20 percent or less ofthe reference sequence over the comparison window. The reference sequence may be a subset of a larger sequence.

[032] As used herein, the twenty conventional amino acids and their abbreviations follow conventional usage. See Immunology - A Synthesis

2018219977 20 Aug 2018 (2^nd Edition, E.S. Golub and D.R. Gren, Eds., Sinauer Associates, Sunderland, Mass. (1991)), which is incorporated herein by reference. The term amino acid or “amino acid residue,” as used herein, refers to naturally occurring L amino acids or to D amino acids. The commonly used one- and three-letter abbreviations for amino acids are used herein (Bruce Alberts et al., Molecular Biology of the Cell, Garland Publishing, Inc., New York (4th ed. 2002)). Stereoisomers (e.g., D-amino acids) of the twenty conventional amino acids, unnatural amino acids such as α-, α-d [substituted amino acids, N-alkyl amino acids, lactic acid, and other unconventional amino acids may also be suitable components for polypeptides of the present invention. Examples of unconventional amino acids include: 4-hydroxyproline, y carboxyglutamate, ε-Ν,Ν,Ν-trimethyllysine, ε-Ν-acetyl lysine, Ophosphoserine, N-acetylserine, N-formylmethionine, 3-methylhistidine, 5hydroxylysine, σ-Ν-methylarginine, and other similar amino acids and imino acids (e.g., 4-hydroxyproline). In the polypeptide notation used herein, the lefthand direction is the amino terminal direction and the righthand direction is the carboxy-terminal direction, in accordance with standard usage and convention.

[033] Similarly, unless specified otherwise, the lefthand end of single-stranded polynucleotide sequences is the 5' end; the lefthand direction of double-stranded polynucleotide sequences is referred to as the 5' direction. The direction of 5' to 3' addition of nascent RNA transcripts is referred to as the transcription direction. Sequence regions on the DNA strand having the same sequence as the RNA and which are 5' to the 5' end of the RNA transcript are referred to as upstream sequences. Sequence regions on the DNA strand having the same sequence as the RNA and which are 3' to the 3' end of the RNA transcript are referred to as downstream sequences.

[034] As applied to polypeptides, the term substantial identity means that two peptide sequences, when optimally aligned, such as by the programs GAP or BESTFIT using default gap weights, share at least 80 percent sequence identity, preferably at least 90 percent sequence identity, more preferably at least 95, 96, 97, or 98 percent sequence identity, and most preferably at least 99 percent sequence identity. Preferably, residue positions

2018219977 20 Aug 2018 which are not identical differ by conservative amino acid substitutions. As discussed herein, minor variations in the amino acid sequences of antibodies or immunoglobulin molecules are contemplated as being encompassed by the present invention, providing that the variations in the amino acid sequence maintain at least 75%, more preferably at least 80%, 90%, 95%, and most preferably 99%. Conservative amino acid substitutions are those that take place within a family of amino acids that are related in their side chains. Genetically encoded amino acids are generally divided into families: (1) acidic=aspartate, glutamate; (2) basic=lysine, arginine, histidine; (3) nonpolar=alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan; and (4) uncharged polar=glycine, asparagine, glutamine, cysteine, serine, threonine, tyrosine. More preferred families are: serine and threonine are aliphatic-hydroxy family; asparagine and glutamine are an amide-containing family; alanine, valine, leucine and isoleucine are an aliphatic family; phenylalanine, tryptophan, and tyrosine are an aromatic family, and cysteine and methionine as a sulfur-containing side chain family. For example, it is reasonable to expect that an isolated replacement of a leucine with an isoleucine or valine, an aspartate with a glutamate, a threonine with a serine, or a similar replacement of an amino acid with a structurally related amino acid will not have a major effect on the binding or properties of the resulting molecule, especially if the replacement does not involve an amino acid within a framework site. Preferred conservative amino acid substitution groups are: valine-leucine-isoleucine, phenylalanine-tyrosine, lysine-arginine, alanine-valine, glutamic acid-aspartic acid, cysteinemethionine, and asparagine-glutamine.

[035] Preferred amino acid substitutions are those which: (1) reduce susceptibility to proteolysis, (2) reduce susceptibility to oxidation, (3) alter binding affinity for forming protein complexes, (4) alter binding affinities, and (5) confer or modify other physicochemical or functional properties of such analogs. Analogs can include various muteins of a sequence other than the naturally-occurring peptide sequence. For example, single or multiple amino acid substitutions (preferably conservative amino acid substitutions) may be made in the naturally-occurring sequence (preferably in the portion of

2018219977 20 Aug 2018 the polypeptide outside the domain(s) forming intermolecular contacts. A conservative amino acid substitution should not substantially change the structural characteristics ofthe parent sequence (e.g., a replacement amino acid should not tend to break a helix that occurs in the parent sequence, or disrupt other types of secondary structure that characterizes the parent sequence). Examples of art-recognized polypeptide secondary and tertiary structures are described in Proteins, Structures and Molecular Principles (Creighton, Ed., W. H. Freeman and Company, New York (1984)); Introduction to Protein Structure (C. Branden and J. Tooze, eds., Garland Publishing, New York, N.Y. (1991)); and Thornton et at. Nature 354:105 (1991), which are each incorporated herein by reference.

[036] The term analog as used herein refers to polypeptides which are comprised of a segment of at least 25 amino acids that has substantial identity to a portion of an amino acid sequence of a naturally occurring polypeptide and which has at least one of the activities of the naturally occurring polypeptide. Typically, polypeptide analogs comprise a conservative amino acid substitution (or addition or deletion) with respect to the naturally-occurring sequence. Analogs typically are at least 20 amino acids long, preferably at least 50 amino acids long or longer, and can often be as long as a full-length naturally-occurring polypeptide.

[037] Peptide analogs are commonly used in the pharmaceutical industry as non-peptide drugs with properties analogous to those ofthe template peptide. Those types of non-peptide compound are termed peptide mimetics or peptidomimetics. Fauchere, J. Adv. Drug Res. 15:29 (1986); Veber and Freidinger TINS p.392 (1985); and Evans et al. J. Med. Chem. 30:1229 (1987), which are incorporated herein by reference. Such compounds are often developed with the aid of computerized molecular modeling. Peptide mimetics that are structurally similar to therapeutically useful peptides may be used to produce an equivalent therapeutic or prophylactic effect. Generally, peptidomimetics are structurally similar to a paradigm polypeptide (i.e., a polypeptide that has a biochemical property or pharmacological activity), such as human antibody, but have one or more peptide linkages optionally replaced by a linkage selected from the group

2018219977 20 Aug 2018 consisting of: -CH₂NH--, --CH₂S-, -CH₂-CH₂-, --CH=CH-(cis and trans), COCH₂--, --CH(OH)CH₂--, and -CH₂SO~, by methods well known in the art. Systematic substitution of one or more amino acids of a consensus sequence with a D-amino acid of the same type (e.g., D-lysine in place of L-lysine) may be used to generate more stable peptides. In addition, constrained peptides comprising a consensus sequence or a substantially identical consensus sequence variation may be generated by methods known in the art (Rizo and Gierasch Ann. Rev. Biochem. 61:387 (1992), incorporated herein by reference); for example, by adding internal cysteine residues capable of forming intramolecular disulfide bridges which cyclize the peptide.

[038] Preferred amino- and carboxy-termini of fragments or analogs occur near boundaries of functional domains. Structural and functional domains can be identified by comparison of the nucleotide and/or amino acid sequence data to public or proprietary sequence databases. Preferably, computerized comparison methods are used to identify sequence motifs or predicted protein conformation domains that occur in other proteins of known structure and/or function. Methods to identify protein sequences that fold into a known three-dimensional structure are known (see Bowie et al. Science 253:164 (1991)), Those of skill in the art can recognize sequence motifs and structural conformations that may be used to define structural and functional domains in accordance with the invention, [039] The term “specific binding agent” refers to a natural or nonnatural molecule that specifically binds to a target. Examples of specific binding agents include, but are not limited to, proteins, peptides, nucleic acids, carbohydrates, lipids, and small molecule compounds. In certain embodiments, a specific binding agent is an antibody. In certain embodiments, a specific binding agent is an antigen binding region.

[040] The term “specific binding agent to an EGFr polypeptide” refers to a specific binding agent that specifically binds any portion of an

EGFr polypeptide. In certain embodiments, a specific binding agent to an

EGFr polypeptide is an antibody to an EGFr polypeptide. In certain embodiments, a specific binding agent to an EGFr polypeptide is an antigen binding region. In certain embodiments, a specific binding agent to an EGFr

2018219977 20 Aug 2018 polypeptide is an antibody to EGFr. In certain embodiments, a specific binding agent to an EGFr polypeptide is panitumumab.

[041] The term “specific binding agent to a mutant K-ras polypeptide” refers to a specific binding agent that specifically binds any portion of a mutant K-ras polypeptide. In certain embodiments, a specific binding agent to a mutant K-ras polypeptide is an antibody to a mutant K-ras polypeptide. In certain embodiments, a specific binding agent to a mutant Kras polypeptide is an antigen binding region.

[042] The term “specifically binds refers to the ability of a specific binding agent to bind to a target with greater affinity than it binds to a nontarget. In certain embodiments, specific binding refers to binding for a target with an affinity that is at least 10, 50, 100, 250, 500, or 1000 times greater than the affinity for a non-target. In certain embodiments, affinity is determined by an affinity ELISA assay. In certain embodiments, affinity is determined by a BIAcore assay. In certain embodiments, affinity is determined by a kinetic method. In certain embodiments, affinity is determined by an equilibrium/solution method. In certain embodiments, an antibody is said to specifically bind an antigen when the dissociation constant between the antibody and one or more of its recognized epitopes is <1 μΜ, preferably < 100 nM and most preferably < 10 nM.

[043] “Native antibodies and immunoglobulins”, in certain instances, are usually heterotetrameric glycoproteins of about 150,000 daltons, composed of two identical light (L) chains and two identical heavy (H) chains. Each light chain is linked to a heavy chain by one covalent disulfide bond, while the number of disulfide linkages varies between the heavy chains of different immunoglobulin isotypes. Each heavy and light chain also has regularly spaced intrachain disulfide bridges. Each heavy chain has at one end a variable domain (VH) followed by a number of constant domains. Each light chain has a variable domain at one end (VL) and a constant domain at its other end; the constant domain of the light chain is aligned with the first constant domain of the heavy chain, and the light chain variable domain is aligned with the variable domain of the heavy chain. Particular amino acid residues are believed to form an interface between the light- and heavy-chain

2018219977 20 Aug 2018 variable domains (Chothia et al. J. Mol. Biol. 186:651 (1985; Novotny and

Haber, Proc. Natl. Acad. Sci. U.S.A. 82:4592 (1985); Chothia et al., Nature

342:877-883 (1989)).

[044] The term antibody refers to both an intact antibody and a antigen binding fragment thereof which competes with the intact antibody for specific binding. “Antigen binding fragment thereof’ refers to a portion or fragment of an intact antibody molecule, wherein the fragment retains the antigen-binding function. Binding fragments are produced by recombinant DNA techniques, or by enzymatic or chemical cleavage of intact antibodies such as by cleavage with papain. Binding fragments include Fab, Fab', F(ab')₂, Fv, single-chain antibodies (scFv), Fd' and Fd fragments. Methods for producing the various fragments from monoclonal antibodies are well known to those skilled in the art (see, e.g., Pluckthun, 1992, Immunol. Rev. 130:151-188). An antibody other than a bispecific” or blfunctional antibody is understood to have each of its binding sites be identical. An antibody substantially inhibits adhesion of a receptor to a counterreceptor when an excess of antibody reduces the quantity of receptor bound to counterreceptor by at least about 20%, 40%, 60%, or 80%, and more usually greater than about 85%, 90%, 95%, 96%, 97%, 98%, or 99% (as measured in an in vitro competitive binding assay).

[045] An “isolated” antibody is one which has been identified and separated and/or recovered from a component of its natural environment. Contaminant components of its natural environment are materials which would interfere with diagnostic or therapeutic uses for the antibody, and may include enzymes, hormones, and other proteinaceous or nonproteinaceous solutes. In preferred embodiments, the antibody will be purified (1) to greater than 95% by weight of antibody as determined by the Lowry method, and terminal or internal amino acid sequencing by use of a spinning cup sequenator, or (2) to homogeneity by SDS-PAGE under reducing or nonreducing conditions using Coomassie blue or, preferably, silver stain. An isolated antibody includes the antibody in situ within recombinant cells since at least one component of the antibody’s natural environment will not be

2018219977 20 Aug 2018 present. Ordinarily, however, isolated antibody will be prepared by at least one purification step.

[046] The term “variable” refers to the fact that certain portions of the variable domains differ extensively in sequence among antibodies and are used in the binding and specificity of each particular antibody for its particular antigen. However, the variability is not evenly distributed throughout the variable domains of antibodies. It is concentrated in three segments called complementarity-determining regions (CDRs) or hypervariable regions both in the light-chain and heavy-chain variable domains. The more highly conserved portions of variable domains are called the framework (FR). The variable domains of native heavy and light chains each comprise four FR regions, largely adopting a β-sheet configuration, connected by three CDRs, which form loops connecting, and in some cases forming part of, the β-sheet structure. The CDRs in each chain are held together in close proximity by the FR regions and, with the CDRs from the other chain, contribute to the formation of the antigen-binding site of antibodies (see Kabat et al. (1991). The constant domains are not involved directly in binding an antibody to an antigen, but exhibit various effector functions, such as participation of the antibody in antibody-dependent cellular toxicity.

[047] “Fv is the minimum antibody fragment which contains a complete antigen-recognition and binding site. In a two-chain Fv species, this region comprises a dimer of one heavy- and one light-chain variable domain in tight, non-covalent association. In a single-chain Fv species, one heavyand one light-chain variable domain can be covalently linked by a flexible peptide linker such that the light and heavy chains can associate in a “dimeric’’ structure analogous to that in a two-chain Fv species. It is in this configuration that the three CDRs of each variable domain interact to define an antigen-binding site on the surface of the VH-VL dimer. Collectively, the six CDRs confer antigen-binding specificity on the antibody. However, even a single variable domain (or half of an Fv comprising only three CDRs specific for an antigen) has the ability to recognize and bind antigen, although at a lower affinity than the entire binding site.

2018219977 20 Aug 2018 [048] The term “hypervariable region’’ when used herein refers to the amino acid residues of an antibody which are responsible for antigenbinding. The hypervariable region generally comprises amino acid residues from a “complementarity determining region” or “CDR” (e.g. residues 24-34 (L1), 50-62 (L2), and 89-97 (L3) in the light chain variable domain and 31-55 (H1), 50-65 (H2) and 95-102 (H3) in the heavy chain variable domain; Kabat et al., Sequences of Proteins of Immunological Interest, 5^th Ed. Public Health Service, National Institutes of Health, Bethesda, MD. (1991)) and/or those residues from a “hypervariable loop” (e.g. residues 26-32 (L1), 50-52 (L2) and 91-96 (L3) in the light chain variable domain and 26-32 ((H1), 53-55 (H2) and 96-101 (H3) in the heavy chain variable domain; Chothia and Lesk J. Mol. Biol 196:901-917 (1987)), “Framework Region” or “FR” residues are those variable domain residues other than the hypervariable region residues as herein defined.

[049] The term “complementarity determining regions” or “CDRs, when used herein, refers to parts of immunological receptors that make contact with a specific ligand and determine its specificity. The CDRs of immunological receptors are the most variable part of the receptor protein, giving receptors their diversity, and are carried on six loops at the distal end of the receptor's variable domains, three loops coming from each ofthe two variable domains of the receptor.

[050] “Antibody-dependent cell-mediated cytotoxicity and “ADCC” refer to a cell-mediated reaction in which non-specific cytotoxic cells that express Fc receptors (FcRs) (e.g. Natural Killer (NK) cells, neutrophils, and macrophages) recognize bound antibody on a target cell and subsequently cause lysis ofthe target cell. The primary cells for mediating ADCC, NK cells, express FcyRIII only, whereas monocytes express FcyRI, FcyRII and FcyRIII. Fc expression on hematopoietic cells is summarized in Table 3 on page 464 of Ravetch and Kinet, Annu. Rev. Immunol 9:457-92 (1991). To assess ADCC activity of a molecule of interest, an in vitro ADCC assay, such as that described in US Patent No. 5,500,362, or 5,821,337 may be performed. Useful effector cells for such assays include peripheral blood mononuclear cells (PBMC) and Natural Killer (NK) cells. Alternatively, or

2018219977 20 Aug 2018 additionally, ADCC activity of the molecule of interest may be assessed in vivo, e.g., in an animal model such as that disclosed in Clynes et al. PNAS (USA) 95:652-656 (1988).

[051] The term “epitope” includes any protein determinant capable of specific binding to an immunoglobulin and/or T-cell receptor. Epitopic determinants usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics.

[052] The term agent is used herein to denote a chemical compound, a mixture of chemical compounds, a biological macromolecule, or an extract made from biological materials.

[053] As used herein, the terms label or labeled refers to incorporation of a detectable marker, e.g., by incorporation of a radiolabeled amino acid or attachment to a polypeptide of biotinyl moieties that can be detected by marked avidin (e.g., streptavidin containing a fluorescent marker or enzymatic activity that can be detected by optical or colorimetric methods). In certain situations, the label or marker can also be therapeutic. Various methods of labeling polypeptides and glycoproteins are known in the art and may be used. Examples of labels for polypeptides include, but are not limited to, the following: radioisotopes or radionuclides (e.g., ³H, ¹⁴C, ^1SN, ³⁵S, ⁹⁰Y, ⁹⁹Tc, ¹¹¹ln, ¹²⁵l,¹³¹1), fluorescent labels (e.g., FITC, rhodamine, lanthanide phosphors), enzymatic labels (e.g., horseradish peroxidase, β-galactosidase, luciferase, alkaline phosphatase), chemiluminescent groups, biotinyl groups, and predetermined polypeptide epitopes recognized by a secondary reporter (e.g., leucine zipper pair sequences, binding sites for secondary antibodies, metal binding domains, epitope tags). In some embodiments, labels are attached by spacer arms of various lengths to reduce potential steric hindrance.

[054] The term pharmaceutical agent or drug as used herein refers to a chemical compound or composition capable of inducing a desired therapeutic effect when properly administered to a patient. Other chemistry terms herein are used according to conventional usage in the art, as

2018219977 20 Aug 2018 exemplified by The McGraw-Hill Dictionary of Chemical Terms (Parker, S.,

Ed., McGraw-Hill, San Francisco (1985)), incorporated herein by reference).

[055] The term antineoplastic agent is used herein to refer to agents that have the functional property of inhibiting a development or progression of a neoplasm in a human, particularly a malignant (cancerous) lesion, such as a carcinoma, sarcoma, lymphoma, or leukemia. Inhibition of metastasis is frequently a property of antineoplastic agents. In certain embodiments, an antineoplastic agent is panitumumab.

[056] As used herein, substantially pure means an object species is the predominant species present (i.e., on a molar basis it is more abundant than any other individual species in the composition), and preferably a substantially purified fraction is a composition wherein the object species comprises at least about 50 percent (on a molar basis) of all macromolecular species present. Generally, a substantially pure composition will comprise more than about 80 percent of all macromolecular species present in the composition, more preferably more than about 85%, 90%, 95%, 96, 97, 98, or 99%. Most preferably, the object species is purified to essential homogeneity (contaminant species cannot be detected in the composition by conventional detection methods) wherein the composition consists essentially of a single macromolecular species.

[057] The term patient includes human and animal subjects.

[058] The terms “mammal” and “animal” for purposes of treatment refers to any animal classified as a mammal, including humans, domestic and farm animals, and zoo, sports, or pet animals, such as dogs, horses, cats, cows, etc. Preferably, the mammal is human.

[059] The term disease state” refers to a physiological state of a cell or of a whole mammal in which an interruption, cessation, or disorder of cellular or body functions, systems, or organs has occurred.

[060] The terms “treat or “treatment” refer to both therapeutic treatment and prophylactic or preventative measures, wherein the object is to prevent or slow down (lessen) an undesired physiological change or disorder, such as the development or spread of cancer. For purposes of this invention, beneficial or desired clinical results include, but are not limited to, alleviation

2018219977 20 Aug 2018 of symptoms, diminishment of extent of disease, stabilized (/. e., not worsening) state of disease, delay or slowing of disease progression, amelioration or palliation of the disease state, and remission (whether partial or total), whether detectable or undetectable. “Treatment” can also mean prolonging survival as compared to expected survival if not receiving treatment. Those in need of treatment include those already with the condition or disorder as well as those prone to have the condition or disorder or those in which the condition or disorder Is to be prevented.

[061] The term responsive as used herein means that a patient or tumor shows a complete response or a partial response after administering an agent, according to RECIST (Response Evaluation Criteria in Solid Tumors). The term “nonresponsive” as used herein means that a patient or tumor shows stable disease or progressive disease after administering an agent, according to RECIST. RECIST is described, e.g., in Therasse et al., February 2000, “New Guidelines to Evaluate the Response to Treatment in Solid Tumors,” J. Natl. Cancer Inst. 92(3): 205-216, which is incorporated by reference herein in its entirety. Exemplary agents include specific binding agents to an EGFr polypeptide, including but not limited to, antibodies to EGFr.

[062] A “disorder” is any condition that would benefit from one or more treatments. This includes chronic and acute disorders or disease including those pathological conditions which predispose the mammal to the disorder in question. Non-limiting examples of disorders to be treated herein include benign and malignant tumors, leukemias, and lymphoid malignancies, in particular breast, rectal, ovarian, stomach, endometrial, salivary gland, kidney, colon, thyroid, pancreatic, prostate or bladder cancer. A preferred disorder to be treated in accordance with the present invention is a malignant tumor, such as cervical carcinomas and cervical intraepithelial squamous and glandular neoplasia, renal cell carcinoma (RCC), esophageal tumors, and carcinoma-derived cell lines.

[063] A “disease or condition related to an EGFr polypeptide” includes one or more of the following: a disease or condition caused by an

EGFr polypeptide; a disease or condition contributed to by an EGFr

2018219977 20 Aug 2018 polypeptide; and a disease or condition that is associated with the presence of an EGFr polypeptide. In certain embodiments, a disease or condition related to an EGFr polypeptide is a cancer. Exemplary cancers include, but are not limited to, non small cell lung carcinoma, breast, colon, gastric, brain, bladder, head and neck, ovarian, and prostate carcinomas.

[064] A “disease or condition related to a mutant K-ras polypeptide” includes one or more of the following: a disease or condition caused by a mutant K-ras polypeptide; a disease or condition contributed to by a mutant K-ras polypeptide; a disease or condition that causes a mutant Kras polypeptide; and a disease or condition that is associated with the presence of a mutant K-ras polypeptide. In certain embodiments, the disease or condition related to a mutant K-ras polypeptide may exist in the absence of the mutant K-ras polypeptide. In certain embodiments, the disease or condition related to a mutant K-ras polypeptide may be exacerbated by the presence of a mutant K-ras polypeptide. In certain embodiments, a disease or condition related to a mutant K-ras polypeptide is a cancer. Exemplary cancers include, but are not limited to, non small cell lung carcinoma, breast, colon, gastric, brain, bladder, head and neck, ovarian, and prostate carcinomas.

[065] In “combined therapy,” patients are treated with a specific binding agent for a target antigen in combination with a chemotherapeutic or antineoplastic agent and/or radiation therapy. In certain embodiments, the specific binding agent is panitumumab. Protocol designs will address effectiveness as assessed by reduction in tumor mass as well as the ability to reduce usual doses of standard chemotherapy. These dosage reductions will allow additional and/or prolonged therapy by reducing dose-related toxicity of the chemotherapeutic agent.

[066] “Monotherapy” refers to the treatment of a disorder by administering immunotherapy to patients without an accompanying chemotherapeutic or antineoplastic agent. In certain embodiments, monotherapy comprises administering panitumumab in the absence of a chemotherapeutic or antineoplastic agent and/or radiation therapy.

2018219977 20 Aug 2018

Certain Embodiments [067] In certain embodiments, a method of diagnosing a disease or condition which is related to one or more K-ras mutations in a subject is provided.

[068] In certain embodiments, a method of diagnosing a disease or condition which is related to one or more K-ras mutations in a subject comprises: (a) determining the presence or amount of expression of a mutant K-ras polypeptide in a sample from the subject; and (b) diagnosing a disease or condition which is related to one or more K-ras mutations based on the presence or amount of expression of the polypeptide. In certain embodiments, a method of diagnosing a disease or condition which is related to one or more K-ras mutations in a subject comprises: (a) determining the presence or amount of transcription or translation of a mutant K-ras polynucleotide in a sample from the subject; and (b) diagnosing a disease or condition which is related to one or more K-ras mutations based on the presence or amount of transcription or translation of the polynucleotide. In certain embodiments, the disease or condition is cancer.

[069] In certain embodiments, a method of diagnosing a disease or condition which is related to one or more K-ras mutations in a subject comprises: (a) determining the presence or amount of expression of a polypeptide comprising at least one amino acid sequence selected from SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, and SEQ ID NO: 18; and (b) diagnosing a disease or condition which is related to one or more Κ-ras mutations based on the presence or amount of expression of the polypeptide. In certain embodiments, a method of diagnosing a disease or condition which is related to one or more K-ras mutations in a subject comprises: (a) determining the presence or amount of transcription or translation of a polynucleotide encoding at least one amino acid sequence selected from SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, and SEQ ID NO: 18 in a sample from the subject; and (b) diagnosing a disease or condition which is related to one or more K-ras mutations based on the presence or amount of transcription or translation of

2018219977 20 Aug 2018 the polynucleotide. In certain embodiments, the disease or condition is cancer.

[070] In certain embodiments, a method of diagnosing a susceptibility to a disease or condition which is related to one or more K-ras mutations in a subject is provided. In certain embodiments, a method of diagnosing a susceptibility to a disease or condition which is related to one or more K-ras mutations in a subject comprises: (a) determining the presence or amount of expression of a mutant K-ras polypeptide in a sample from the subject; and (b) diagnosing a susceptibility to a disease or condition which is related to one or more K-ras mutations based on the presence or amount of expression of the polypeptide. In certain embodiments, a method of diagnosing a susceptibility to a disease or condition which is related to one or more K-ras mutations in a subject comprises: (a) determining the presence or amount of transcription or translation of a mutant K-ras polynucleotide in a sample from the subject; and (b) diagnosing a susceptibility to a disease or condition which is related to one or more K-ras mutations based on the presence or amount of transcription or translation of the polynucleotide. In certain embodiments, the disease or condition is cancer.

[071] In certain embodiments, a method of diagnosing a susceptibility to a disease or condition which is related to one or more K-ras mutations in a subject comprises: (a) determining the presence or amount of expression of a polypeptide comprising at least one amino acid sequence selected from SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, and SEQ ID NO: 18 in a sample from the subject; and (b) diagnosing a susceptibility to a disease or condition which is related to one or more K-ras mutations based on the presence or amount of expression of the polypeptide. In certain embodiments, a method of diagnosing a susceptibility to a disease or condition which is related to one or more K-ras mutations in a subject comprises: (a) determining the presence or amount of transcription or translation of a polynucleotide encoding at least one amino acid sequence selected from SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, and SEQ ID NO: 18 in a

2018219977 20 Aug 2018 sample from the subject; and (b) diagnosing a susceptibility to a disease or condition which is related to one or more K-ras mutations based on the presence or amount of transcription or translation of the polypeptide. In certain embodiments, the disease or condition is cancer.

[072] In certain embodiments, a method of determining the presence or absence of a polynucleotide encoding a mutant K-ras polypeptide is provided. In certain embodiments, a method of determining the presence or absence of a polynucleotide encoding a mutant K-ras polypeptide in a sample comprises (a) exposing a sample to a probe which hybridizes to a polynucleotide encoding a region of a mutant K-ras polypeptide, wherein the region comprises at least one K-ras mutation selected from G12S, G12V, G12D, G12A, G12C, G13A, G13D, and T20M, and (b) determining the presence or absence of a polynucleotide encoding a mutant K-ras polypeptide in the sample. In certain embodiments, a method of determining the presence or absence of a mutant K-ras polypeptide in a sample comprises (a) exposing a sample to a probe which hybridizes to a polynucleotide encoding a region of a mutant K-ras polypeptide, wherein the region comprises at least one K-ras mutation selected from G12S, G12V, G12D, G12A, G12C, G13A, G13D, and T20M, and (b) determining the presence or absence of a mutant K-ras polypeptide in the sample.

[073] In certain embodiments, a method for establishing a mutant K-ras population profile in a specific population of individuals is provided comprising: (a) determining the presence of at least one K-ras mutation in a genetic profile ofthe individuals in a population; and (b) establishing a relationship between mutant K-ras genetic profiles and the individuals. In certain such embodiments, the specific characteristics of the individuals include a susceptibility to developing a disease or condition which is related to a K-ras mutation. In certain such embodiments, the specific characteristics of the individuals include exhibiting a disease or condition which is related to an K-ras mutation.

[074] In certain embodiments, a method of predicting nonresponsiveness to treatment with a specific binding agent to an EGFr polypeptide in a subject suffering from cancer is provided, comprising

2018219977 20 Aug 2018 determining the presence or absence of K-ras mutation G12S in the subject. In certain embodiments, a specific binding agent to an EGFr polypeptide is an antibody to EGFr. In certain such embodiments, the antibody is panitumumab.

[075] In certain embodiments, a method of predicting non responsiveness to treatment with a specific binding agent to an EGFr polypeptide in a subject suffering from cancer is provided, comprising determining the presence or absence of K-ras mutation G12V in the subject. In certain embodiments, a specific binding agent to an EGFr polypeptide is an antibody to EGFr. In certain such embodiments, the antibody is panitumumab.

[076] In certain embodiments, a method of predicting nonresponsiveness to treatment with a specific binding agent to an EGFr polypeptide in a subject suffering from cancer is provided, comprising determining the presence or absence of K-ras mutation G12D in the subject. In certain embodiments, a specific binding agent to an EGFr polypeptide is an antibody to EGFr. In certain such embodiments, the antibody is panitumumab.

[077] In certain embodiments, a method of predicting nonresponsiveness to treatment with a specific binding agent to an EGFr polypeptide in a subject suffering from cancer is provided, comprising determining the presence or absence of K-ras mutation G12A in the subject. In certain embodiments, a specific binding agent to an EGFr polypeptide is an antibody to EGFr. In certain such embodiments, the antibody is panitumumab.

[078] In certain embodiments, a method of predicting non responsiveness to treatment with a specific binding agent to an EGFr polypeptide in a subject suffering from cancer is provided, comprising determining the presence or absence of K-ras mutation G12C in the subject. In certain embodiments, a specific binding agent to an EGFr polypeptide is an antibody to EGFr. In certain such embodiments, the antibody is panitumumab.

2018219977 20 Aug 2018 [079] In certain embodiments, a method of predicting nonresponsiveness to treatment with a specific binding agent to an EGFr polypeptide in a subject suffering from cancer is provided, comprising determining the presence or absence of K-ras mutation G13A in the subject. In certain embodiments, a specific binding agent to an EGFr polypeptide is an antibody to EGFr. In certain such embodiments, the antibody is panitumumab.

[080] In certain embodiments, a method of predicting nonresponsiveness to treatment with a specific binding agent to an EGFr polypeptide in a subject suffering from cancer is provided, comprising determining the presence or absence of K-ras mutation G13D in the subject. In certain embodiments, a specific binding agent to an EGFr polypeptide is an antibody to EGFr. In certain such embodiments, the antibody is panitumumab.

[081] In certain embodiments, a method of predicting nonresponsiveness to treatment with a specific binding agent to an EGFr polypeptide in a subject suffering from cancer is provided, comprising determining the presence or absence of K-ras mutation T20M in the subject. In certain embodiments, a specific binding agent to an EGFr polypeptide is an antibody to EGFr. In certain such embodiments, the antibody is panitumumab.

[082] In certain embodiments, a method of predicting non responsiveness to treatment with a specific binding agent to an EGFr polypeptide in a subject suffering from cancer is provided, comprising determining the presence or absence of a K-ras mutation at amino acid 12 of K-ras and/or amino acid 13 and/or amino acid 20 of K-ras in the subject. In certain embodiments, a specific binding agent to an EGFr polypeptide is an antibody to EGFr. In certain such embodiments, the antibody is panitumumab.

[083] In certain embodiments, a kit for detecting a polynucleotide encoding a mutant K-ras polypeptide in a subject is provided. In certain such embodiments, the kit comprises a probe which hybridizes to a polynucleotide encoding a region of a mutant K-ras polypeptide, wherein the region

2018219977 20 Aug 2018 comprises at least one K-ras mutation selected from G12S, G12V, G12D, G12A, G12C, G13A, G13D, and T20M. In certain embodiments, the kit further comprises two or more amplification primers. In certain embodiments, the kit further comprises a detection component. In certain embodiments, the kit further comprises a nucleic acid sampling component.

[084] In certain embodiments, nonresponsiveness to treatment with a specific binding agent to an EGFr polypeptide is determined using RECIST (Response Evaluation Criteria in Solid Tumors). Complete response and partial response according to RECIST are both considered to be responsive to treatment with a specific binding agent to an EGFr polypeptide. Stable disease and progressive disease are both considered to be nonresponsive to treatment with a specific binding agent to an EGFr polypeptide. RECIST is known in the art and it described, e.g., in Therasse et al., February 2000, “New Guidelines to Evaluate the Response to Treatment in Solid Tumors,” J. Natl. Cancer Inst. 92(3): 205-216, which is incorporated by reference herein for any purpose.

[085] In certain embodiments, a K-ras mutation is detected. In certain embodiments, a K-ras mutation is detected by detecting the mutant Kras polynucleotide . In certain embodiments, a K-ras mutation is detected by detecting the mutant K-ras polypeptide.

[086] Certain methods of detecting a mutation in a polynucleotide are known in the art. Certain exemplary such methods include, but are not limited to, sequencing, primer extension reactions, electrophoresis, picogreen assays, oligonucleotide ligation assays, hybridization assays, TaqMan assays, SNPlex assays, and assays described, e.g., in U.S. Patent Nos. 5,470,705, 5,514,543, 5,580,732, 5,624,800, 5,807,682, 6,759,202, 6,756,204, 6,734,296, 6,395,486, and U.S. Patent Publication No. US 20030190646 A1.

[087] In certain embodiments, detecting a mutation in a polynucleotide comprises first amplifying a polynucleotide that may comprise the mutation. Certain methods for amplifying a polynucleotide are known in the art. Such amplification products may be used in any of the methods

2018219977 20 Aug 2018 described herein, or known in the art, for detecting a mutation in a polynucleotide.

[088] Certain methods of detecting a mutation in a polypeptide are known in the art. Certain exemplary such methods include, but are not limited to, detecting using a specific binding agent specific for the mutant polypeptide. Other methods of detecting a mutant polypeptide include, but are not limited to, electrophoresis and peptide sequencing.

[089] Certain exemplary methods of detecting a mutation in a polynucleotide and/or a polypeptide are described, e.g., in Schimanski et al. (1999) Cancer Res., 59: 5169-5175; Nagasaka et al. (2004) J. Clin. Oncol., 22: 4584-4596; PCT Publication No. WO 2007/001868 Al; U.S. Patent Publication No. 2005/0272083 A1; and Lievre et al. (2006) Cancer Res. 66; 3992-3994.

[090] In certain embodiments, microarrays comprising one or more polynucleotides encoding one or more mutant K-ras polypeptides are provided. In certain embodiments, microarrays comprising one or more polynucleotides complementary to one or more polynucleotides encoding one or more mutant K-ras polypeptides are provided.

[091] In certain embodiments, the presence or absence of one or more mutant K-ras polynucleotides in two or more cell or tissue samples is assessed using microarray technology. In certain embodiments, the quantity of one or more mutant K-ras polynucleotides in two or more cell or tissue samples is assessed using microarray technology.

[092] In certain embodiments, the presence or absence of one or more mutant K-ras polypeptides in two or more cell or tissue samples is assessed using microarray technology. In certain such embodiments, mRNA is first extracted from a cell or tissue sample and is subsequently converted to cDNA, which is hybridized to the microarray. In certain such embodiments, the presence or absence of cDNA that is specifically bound to the microarray is indicative of the presence or absence of the mutant K-ras polypeptide. In certain such embodiments, the expression level of the one or more mutant Kras polypeptides is assessed by quantitating the amount of cDNA that is specifically bound to the microarray.

2018219977 20 Aug 2018 [093] In certain embodiments, microarrays comprising one or more specific binding agents to one or more mutant K-ras polypeptides are provided. In certain such embodiments, the presence or absence of one or more mutant K-ras polypeptides in a cell or tissue is assessed. In certain such embodiments, the quantity of one or more mutant K-ras polypeptides in a cell or tissue is assessed.

[094] The following examples, including the experiments conducted and results achieved are provided for illustrative purpose only and are not to be construed as limiting upon the claims.

EXAMPLES

EXAMPLE 1

IDENTIFICATION OF K-RAS EXON2 MUTATIONS IN COLORECTAL ADENOCARCINOMA TUMOR SAMPLES [095] To identify mutations in K-ras exon 2 associated with colorectal adenocarcinoma (“CRC”), the K-ras exon 2 was amplified from thirty-seven CRC patient tumors. Double-blinded tumor samples from thirtyseven patients enrolled in a CRC trial were obtained prior to patient treatment with panitumumab. The study was a multi-center, open label, single-arm clinical trial. Patients were treated with 2.5 mg/kg panitumumab weekly, repeated in an 8 week cycle until disease progression. Tumor assessments were preformed by blinded central radiology review (a panel of at least 2 radiologists) using RECIST criteria and confirmed no less that 4 weeks after the response criteria were first met. Each isolated exon was sequenced to identify any alterations from the wild-type sequences for those exons.

[096] CRC tumor samples from thirty-seven patients were collected. A portion of each tumor sample was stained to identify the amount of EGFr expression of the tumor and rated for staining on a three-point scale (where 3 is the greatest degree of staining). At least 10% of each tumor sample demonstrated a staining level of three. Tumor tissue was separated from adjacent normal tissue, necrotic debris, and stroma by macro dissection of forma I in-fixed, paraffin-embedded tissue sections. Trimmed samples were fixed on microscope slides and stored at room temperature.

2018219977 20 Aug 2018

Table 1: CRC Patient Samples

Histology Number	Patient Number	Clinical Trial Patient Number
05H-5699EB	17040	5149
04H-405CSB	17043	5152
04H-406DB	17046	5139
05H-5700NM	17049	5150
05H-5698RBR	17052	5147
05H-5692SM	17055	5093
05H-5783JMY	17061	5143
04H-413EED	17066	5151
05H-5694CS	17069	5141
05H-5689RBA	17075	5072
05H-5697IAM	17078	5145
04H-746XC	18215	5101
04H-747SH	18230	5133
04H-748JA	18244	5097
04H-750GR	18275	5115
04H-752HBM	18321	5105
04H-753JR	18333	5106
04H-754BLB	18347	5113
04H-755JE	18361	5110
04H-758GDM	18396	5120
04H-759JH	18410	5123
04H-7600GM	18422	5124
04H-762GRK	18449	5131
04H-763CHK	18463	5129
04H-765DB	18491	5136
04 H-766LA	18505	5137
04H-768LGL	18526	5119
04H-771WRH	18554	5102
04H-772CEM	18569	5125
04H-773LOO	18583	5096
04H-775BM	18614	5107
04H-776LAP	18628	5108
04H-780GL	18688	5135
04H-781JLK	18702	5121
05H-5686FEC	23458	5030
05H-5690JFF	23481	5079
05H-5696REF	23S34	5144

[097] Genomic DNA was prepared from the sample slides using the Pinpoint Slide DNA Isolation System (Zymo Research, Orange, CA) according to the manufacturer’s protocol. The final isolated genomic DNA product was dissolved in 500 μL water.

[098] The wild-type K-ras polypeptide sequence is shown in Figure 1A (SEQ ID NO: 2; Genbank Accession No. NP_004976). The wild-type Kras cDNA sequence is also shown in Figure 1A (SEQ ID NO: 1; Genbank

2018219977 20 Aug 2018

Accession No. NM_004985). The genomic wild-type K-ras nucleotide sequence is found at Genbank Accession No. NM_004985. Primer sequences for each exon were designed using the intron sequences 5' and 3' to exon 2 in the wild-type K-ras cDNA sequence (SEQ ID NO: 1). The sequences corresponding to exon 2 of human K-ras were amplified by PCR using specific primers: primer 3401-41 (5’- AAGGTACTGGTGGAGTATTTG3’ SEQ ID NO. 19) and primer 3401-44 (5’GTACTCATGAAAATGGTCAGAG-3' SEQ ID NO. 20).

[099] PCR was performed using Taq DNA polymerase (Roche Diagnostics Corp) or equivalent and the following conditions: 5 μΙ_ of 10x Taq buffer, 0.5 μΙ_ of 24 mM MgCI2, 1 pL genomic DNA (approximately 0.5 ng), 7 pL of 2.5 mM dNTPs, 1 pL Taq polymerase (5U) and 29.5 pL ddH₂O were combined and mixed. 6 pL of combined primer stock (10 pM of each each) was added to each tube. The cycle protocol was 1 cycle of 4 minutes at 93 °C; 10 seconds at 93 °C, 30 seconds at 62°C, 30 seconds at 72 °C for 35 cycles; and 1 cycle of 4 minutes at 72°C. At the end of the reaction the temperature was held at 4°C.

[0100] The PCR products for each individual exon were gel-purified. The purified amplified exon sequences were subcloned into a pCR2.1 vector using a TOPO-TA Cloning Kit (Invitrogen Corp) according to the manufacturer’s instructions. E. coli colonies containing the vector and insert exon of interest were picked by a Genetix Colony Picker. Those colonies were grown overnight in liquid medium. Plasmid DNA from each overnight bacterial culture was isolated using a QIAGEN 9600, 3000, or 8000 Bio-robot (Qiagen) according to the manufacturer’s instructions.

[0101] Isolated plasmid DNA containing each exon was sequenced using a BigDye 3.1 Terminator Kit (Applied Biosystems, Inc.) according to the manufacturer’s instructions. Sequencing data was collected using a 3700, 3100, or 3730 Genetic Analyzer (Applied Biosystems, Inc,), and analyzed using the SeQuencher program (GeneCodes Corp.). The exon sequences from the patient samples were compared to the wild-type exon sequences.

[0102] The mutational analysis of the CRC patient tumor samples, identified 13 patients with a K-ras exon 2 mutation (Table 2). Tumor

2018219977 20 Aug 2018 response was assessed using CT or MRI and statistically analyzed using RECIST (Response Evaluation Criteria in Solid Tumors), which provides guidelines for identifying complete response, partial response, stable disease, or progressive disease based on tumor size (see, e.g., Therasse et al., February 2000, New Guidelines to Evaluate the Response to Treatment in Solid Tumors, J. Natl. Cancer Inst. 92(3): 205-216).

Table 2: CRC Patient Samples Result

PTS ID	Mutation	Response
5149	WT	PD
5152	G12S	PD
5139	G12V	PD
5150	WT	PD
5147	WT	PD
5093	G12D	SD
5143	G13D	PD
5151	WT	PD
5141	G12V	PD
5072	WT	PD
5145	WT	SD
5101	WT	SD
5133	WT	SD
5097	WT	PR
5115	G12A	SD
5105	WT	PR
5106	G12D	PD
5113	G12C	PD
5110	WT	SD
5120	WT	PR
5123	WT	SD
5124	WT	PD
5131	WT	PD
5129	WT	SD
5136	WT	PD
5137	G12V	PD
5119	WT	SD
5102	G12V	PD
5125	G12V, T20M	SD
5096	WT	SD
5107	G12D	PD
5108	WT	PD
5135	WT	SD
5121	WT	SD
5030	WT	PR
5079	WT	SD
5144	G12D	PD

[0103] PD stands for progressive disease, PR stands for partial response, and SD stands for stable disease.

2018219977 20 Aug 2018 [0104] Ofthe thirty-seven tumors, 13 had mutations in exon 2 of Kras (36%) and 24 were wild-type in exon 2 of K-ras (64%). Of the 13 tumors with a mutation in exon 2 of K-ras, none (0%) showed a partial response to panitumumab. In contrast, 4 of the tumors with wild-type exon 2 of K-ras (17%) showed partial response to panitumumab. Similarly, only 3 (23%) of the tumors with a mutation in exon 2 showed stable disease after panitumumab treatment, while 11 (46%) ofthe tumors with wild-type exon 2 of K-ras showed stable disease. Finally, 10 (77%) of the tumors with a mutation in exon 2 showed progressive disease after panitumumab treatment, while only 9 (37%) of the tumors with wild-type exon 2 of K-ras showed progressive disease.

[0105] Those data are summarized in Table 3.

Table 3. Summary of CRC patient mutation status and response to panitumumab

	mut+	mut-
PR	0 (0%)	4 (17%)
SD	3 (23%)	11 (46%)
RD	10(77%)	9 (37%)

[0106] In that analysis, a mutation in K-ras exon 2, especially mutations G12S, G12V, G12D, G12A, G12C, G13A, G13D, and T20M, were correlated with nonresponsiveness to panitumumab therapy.

[0107] Other embodiments will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only.

2018219977 20 Aug 2018

Claims

WE CLAIM:

1. A method of predicting whether a patient will be nonresponsive to treatment with a specific binding agent to an EGFr polypeptide, comprising determining the presence or absence of a K-ras mutation in a tumor of the patient, wherein the K-ras mutation is in codon 12 or codon 13 or codon 20; and wherein if a K-ras mutation is present, the patient is predicted to be nonresponsive to treatment with a specific binding agent to an EGFr polypeptide.
2. The method of claim 1, wherein the determining the presence or absence of a K-ras mutation in a tumor comprises amplifying a K-ras nucleic acid from the tumor and sequencing the amplified nucleic acid.
3. The method of claim 1, wherein the specific binding agent to an EGFr polypeptide is an antibody to EGFr.
4. The method of claim 3, wherein the antibody to EGFr is panitumumab.
5. The method of claim 1, wherein the determining the presence or absence of a K-ras mutation in a tumor comprises detecting a mutant K-ras polypeptide in a sample of the tumor using a specific binding agent to a mutant K-ras polypeptide.
6. The method of claim 1, wherein the K-ras mutation is selected from G12S, G12V, G12D, G12A, G12C, G13A, G13D, and T20M.
7. A method of predicting whether a tumor will be nonresponsive to treatment with a specific binding agent to an EGFr polypeptide, comprising determining the presence or absence of a K-ras mutation in a sample of said tumor, wherein the K-ras mutation is in codon 12 or codon 13 or codon 20; and wherein the presence of the K-ras mutation indicates that the tumor will be nonresponsive to treatment with a specific binding agent to an EGFr polypeptide.
8. The method of claim 7, wherein the determining the presence or absence of a K-ras mutation in a sample of said tumor comprises amplifying K-ras nucleic acid from the tumor and sequencing the amplified nucleic acid.
9. The method of claim 7, wherein the specific binding agent to an EGFr polypeptide is an antibody to EGFr.

2018219977 20 Aug 2018
10. The method of claim 9, wherein the antibody to EGFr is panitumumab.
11. The method of claim 7, wherein the determining the presence or absence of a K-ras mutation in the sample of said tumor comprises detecting a mutant K-ras polypeptide using a specific binding agent to a mutant K-ras polypeptide.

12. The method of claim 7, wherein the K-ras mutation is selected from G12S, G12V, G12D, G12A, G12C, G13A, G13D, and T20M.

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2018219977 20 Aug 2018

118 PCT seq list SEQUENCE LISTING <110> Freeman, Daniel Juan, Todd Radinsky, Robert <120> K-RAS MUTATIONS AND ΑΝΤΙ-EGFR ANTIBODY THERAPY <130> 6843.118-304 <150> 60/906,943 <151> 2007-03-13 <160> 18 <170> Patentin version 3.3 <210> 1 <211> 567 <212> DNA <213> Homo sapiens <400> 1

atgactgaat ataaacttgt ggtagttgga gctggtggcg taggcaagag tgccttgacg 60 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac 120 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 180 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 240 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 300 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 360 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 420 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt 480 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag 540 tcaaagacaa agtgtgtaat tatgtaa 567 <210> 2 <211> 188 <212> PRT <213> Homo sapiens <400> 2 Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Gly val Gly Lys 1 5 10 15 Ser Ala Leu Thr lie G1 n Leu lie Gin Asn His Phe val Asp Glu Tyr 20 25 30 Asp Pro Thr lie Glu Asp Ser Tyr Arg Lys Gin val val lie Asp Gly 35 40 45 Glu Thr Cys Leu Leu Asp lie Leu Asp Thr Ala Gly Gin Glu Glu Tyr 50 55 60 Ser Ala Met Arg Asp G1 n Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys

Page 1

2018219977 20 Aug 2018

118 PCT seq list

Val Phe Ala Ile Asn 85 Asn Thr Lys Ser Phe Glu Asp 90 Ile His His 95 Tyr Arg Glu G1 n lie Lys Arg Val Lys Asp Ser Glu Asp val Pro Met val 100 105 110 Leu Val Gly Asn Lys Cys Asp Leu Pro ser Arg Thr val Asp Thr Lys 115 120 125 Gin Al a Gin Asp Leu Ala Arg ser Tyr Gly lie Pro Phe lie Glu Thr 130 135 140 Ser Ala Lys Thr Arg Gin Gly val Asp Asp Ala Phe Tyr Thr Leu val 145 150 15 5 160 Arg Glu lie Arg Lys His Lys Glu Lys Met Ser Lys Asp Gly Lys Lys 165 170 175 Lys Lys Lys Lys ser Lys Thr Lys cys Val lie Met 180 185

<210> <211> <212> <213> 3 567 DNA Homo sapiens <400> 3

atgactgaat ataaacttgt ggtagttgga gctagtggcg taggcaagag tgccttgacg 60 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac 120 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 180 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 240 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 300 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 360 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 420 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt 480 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag 540 tcaaagacaa agtgtgtaat tatgtaa 567

<210> 4 <211> 188 <212> PRT <213> Homo sapi ens <400> 4 Met Thr Glu Tyr Lys Leu Val Val val Gly Ala Ser Gly val Gly Lys 1 5 10 15

Page 2

118 PCT seq list

2018219977 20 Aug 2018

Ser Ala Leu Thr lie Gln Leu lie Gln 25 Asn Hi s Phe Val Asp 30 Glu Tyr 20 Asp Pro Thr ile Glu Asp Ser Tyr Arg Lys Gln Val Val lie Asp Gly 35 40 45 Glu Thr cys Leu Leu Asp lie Leu Asp Thr Al a Gly Gln Glu Glu Tyr 50 55 60 Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu cys 65 70 75 80 val Phe Ala lie Asn Asn Thr Lys Ser Phe Glu Asp lie His His Tyr 85 90 95 Arg Glu Gln lie Lys Arg Val Lys Asp Ser Glu Asp val Pro Met Val 100 105 110 Leu Val Gly Asn Lys Cys Asp Leu Pro Ser Arg Thr val ASP Thr Lys 115 120 125 Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Phe lie Glu Thr 130 135 140 ser Ala Lys Thr Arg Gln Gly Val Asp Asp Ala Phe Tyr Thr Leu val 145 150 155 160 Arg Glu Ile Arg Lys His Lys Glu Lys Met Ser Lys Asp Gly Lys Lys 165 170 175 Lys Lys Lys Lys ser Lys Thr Lys cys Val lie Met 180 185

<210> <211> <212> <213> 5 567 DNA Homo sapiens <400> 5

atgactgaat ataaacttgt ggtagttgga gctgttggcg taggcaagag tgccttgacg 60 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac 120 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 180 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 240 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 300 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 360 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 420 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt 480 cgagaaattc gaaaacataa agaaaagatg agcaaagatg Page 3 gtaaaaagaa gaaaaagaag 540

2018219977 20 Aug 2018

118 PCT seq list tcaaagacaa agtgtgtaat tatgtaa <210> 6 <211> 188 <212> PRT <213> Homo sapiens <400> 6

Met 1 Thr Glu Tyr Lys Leu val 5 val val Gly 10 Ala Val Gly Val Gly 15 Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe val Asp Glu Tyr 20 25 30 Asp pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln val val Ile Asp Gly 35 40 45 G1 u Thr Cys Leu Leu Asp ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr 50 55 60 Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu cys 65 70 75 80 Val phe Ala Ile Asn Asn Thr Lys Ser phe Glu Asp lie His His Tyr 85 90 95 Arg Glu Gln Ile Lys Arg val Lys Asp Ser Glu Asp val Pro Met Val 100 105 110 Leu val Gly Asn Lys cys Asp Leu Pro Ser Arg Thr val Asp Thr Lys 115 120 125 Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly lie pro Phe Ile G1 u Thr 130 135 140 Ser Al a Lys Thr Arg Gln Gly val Asp Asp Ala Phe Tyr Thr Leu Val 145 150 155 160 Arg Glu Ile Arg Lys Hl s Lys Glu Lys Met Ser Lys Asp Gly Lys Lys 165 170 175 Lys Lys Lys Lys Ser Lys Thr Lys Cys val Ile Met 180 185

<210>7 <211>567 <212> DNA <213> Homo sapiens <400>7 atgactgaat ataaacttgt ggtagttgga gctgatggcg taggcaagag tgccttgacg atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac Page 4

567

120

2018219977 20 Aug 2018

118 pct seq list aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 180 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 240 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 300 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 360 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 420 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt 480 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag 540 tcaaagacaa agtgtgtaat tatgtaa 567

<210> 8 <211> 188 <212> PRT <213> Homo sapiens <400> 8

Met 1 Thr Glu Tyr Lys 5 Leu val val val Gly 10 Ala Asp Gly val Gly 15 Lys ser Ala Leu Thr Ile Gln Leu lie Gln Asn His Phe Val Asp Glu Tyr 20 25 30 Asp Pro Thr Ile Glu Asp ser Tyr Arg Lys Gln val val Ile Asp Gly 35 40 45 Glu Thr cys Leu Leu Asp Ile Leu Asp Thr Ala Gly Gln G1 u Glu Tyr 50 55 60 ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu cys 65 70 75 80 val phe Ala Ile Asn Asn Thr Lys ser Phe Glu Asp lie His His Tyr 85 90 95 Arg Glu Gln Ile Lys Arg val Lys Asp ser Glu Asp Val Pro Met Val 100 105 110 Leu val Gly Asn Lys cys Asp Leu Pro ser Arg Thr val Asp Thr Lys 115 120 125 Gln Ala Gln Asp Leu Ala Arg ser Tyr Gly Ile Pro Phe lie Glu Thr 130 135 140 Ser Ala Lys Thr Arg Gln Gly val Asp Asp Al a Phe Tyr Thr Leu Val 145 150 155 160 Arg G1 u lie Arg Lys His Lys Glu Lys Met Ser Lys Asp Gly Lys Lys

165 170 175

Page 5

2018219977 20 Aug 2018

118 PCT seq list

Lys Lys Lys Lys Ser Lys Thr Lys Cys Val Ile Met

180185 <210>9 <211>567 <212> DNA <213> Homo sapiens

<400> 9 atgactgaat ataaacttgt ggtagttgga gctgctggcg taggcaagag tgccttgacg 60 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac 120 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 180 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 240 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 300 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 360 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 420 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt 480 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag 540 tcaaagacaa agtgtgtaat tatgtaa 567

<210> <211> <212> <213> 10 188 PRT Homo sapiens <400> 10

Met 1 Thr Glu Tyr Lys 5 Leu Val Val val Gly 10 Ala Ala Gly val Gly 15 Lys Ser Al a Leu Thr lie Gln Leu Ile Gln Asn His phe val Asp G1 u Tyr 20 25 30 Asp Pro Thr lie G1 u Asp Ser Tyr Arg Lys Gln val val Ile Asp Gly 35 40 45 Glu Thr cys Leu Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu G1 u Tyr 50 55 60 Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu cys 65 70 75 80 Val phe Al a Ile Asn Asn Thr Lys Ser phe Glu Asp lie His His Tyr 85 90 95 Arg Glu Gln lie Lys Arg Val Lys Asp ser Glu Asp val Pro Met val 100 105 110 Leu val Gly Asn Lys cys Asp Leu Pro ser Arg Thr val Asp Thr Lys

Page 6

2018219977 20 Aug 2018

118 PCT seq list 115 120 125 Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Phe Ile Glu Thr 130 135 140 Ser Al a Lys Thr Arg Gln Gly val Asp Asp Ala Phe Tyr Thr Leu val 145 150 15 5 160 Arg G1 u lie Arg Lys His Lys Glu Lys Met Ser Lys Asp Gly Lys Lys 165 170 175 Lys Lys Lys Lys Ser Lys Thr Lys Cys Val lie Met 180 185

<210> 11 <211> 567 <212> DNA <213> Homo sapiens <400> 11

atgactgaat ataaacttgt ggtagttgga gcttgtggcg taggcaagag tgccttgacg 60 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac 120 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 180 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 240 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 300 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 360 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 420 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt 480 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag 540 tcaaagacaa agtgtgtaat tatgtaa 567

<210> 12 <211> 188 <212> PR.T <213> Homo sapiens <400> 12

Met Thr Glu Tyr Lys Leu val Val Val Gly Ala cys Gly Val Gly Lys 1 5 10 15 ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu Tyr 20 25 30 Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp Gly 35 40 45 Glu Thr Cys Leu Leu Asp lie Leu Asp Thr Ala Gly Gln Glu Glu Tyr

50 55 60

Page 7

118 PCT seq list

2018219977 20 Aug 2018

Ser Ala Met Arg Asp Gin Tyr Met Arg Thr Gly Glu Gly phe Leu cys 65 70 75 80 val phe Ala lie Asn Asn Thr Lys ser Phe Glu Asp lie His His Tyr 85 90 95 Arg Glu Gin lie Lys Arg val Lys Asp ser Glu Asp Val Pro Met val 100 105 110 Leu Val Gly Asn Lys Cys Asp Leu Pro ser Arg Thr val Asp Thr Lys 115 120 125 Gin Ala Gin Asp Leu Ala Arg Ser Tyr Gly lie Pro Phe lie Glu Thr 130 135 140 ser Ala Lys Thr Arg Gin Gly val Asp Asp Ala Phe Tyr Thr Leu Val 145 150 155 160 Arg Glu lie Arg Lys Hi s Lys Glu Lys Met Ser Lys Asp Gly Lys Lys 165 170 175 Lys Lys Lys Lys Ser Lys Thr Lys cys Val lie Met 180 185

<210> <211> <212> <213> 13 567 DNA Homo sapiens <40 0> 13

atgactgaat ataaacttgt ggtagttgga gctggtgccg taggcaagag tgccttgacg 60 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac 120 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 180 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 240 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 300 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 360 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 420 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt 480 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag 540 tcaaagacaa agtgtgtaat tatgtaa 567

<210> 14 <211> 188 <212> prt <213> Homo sapiens <400> 14

Met Thr Glu Tyr Lys Leu val Vai Vai Gly Ala Gly Ala Vai Gly Lys Page 8

2018219977 20 Aug 2018

1 5 118 PCT 10 seq list 15 Ser Ala Leu Thr lie Gin Leu lie Gin Asn His Phe val Asp Glu Tyr 20 25 30 Asp pro Thr lie Glu Asp ser Tyr Arg Lys Gin val val lie Asp Gly 35 40 45 Glu Thr Cys Leu Leu Asp lie Leu Asp Thr Ala Gly Gin Glu Glu Tyr 50 55 60 Ser Ala Met Arg Asp Gin Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys 65 70 75 80 Vai Phe Ala lie Asn Asn Thr Lys ser Phe Glu Asp lie His His Tyr 85 90 95 Arg Glu Gin lie Lys Arg val Lys Asp Ser Glu Asp val Pro Met val 100 105 110 Leu val Gly Asn Lys cys Asp Leu Pro ser Arg Thr val ASp Thr Lys 115 120 125 Gin Ala Gin Asp Leu Ala Arg ser Tyr Gly lie Pro Phe lie Glu Thr 130 135 140 ser Ala Lys Thr Arg Gin Gly val Asp Asp Ala Phe Tyr Thr Leu val 145 150 155 160 Arg Glu lie Arg Lys His Lys Glu Lys Met ser Lys Asp Gly Lys Lys 165 170 175- Lys Lys Lys Lys ser Lys Thr Lys cys val lie Met

180 185

<210> <211> <212> <213> 15 567 DNA Homo sapiens <400> 15

atgactgaat ataaacttgt ggtagttgga gctggtgacg taggcaagag tgccttgacg 60 atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac 120 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 180 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 240 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 300 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 360 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 420 tttattgaaa catcagcaaa gacaagacag ggtgttgatg Page 9 atgccttcta tacattagtt 480

2018219977 20 Aug 2018

118 PCT seq list cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag tcaaagacaa agtgtgtaat tatgtaa <210> 16 <211> 188 <212> PRT <213> Homo sapiens <400> 16

Met Thr Glu Tyr Lys Leu val val val Gly Ala 10 Gly Asp Val Gly 15 Lys 1 5 Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe val Asp G1 u Tyr 20 25 30 Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Val lie Asp Gly 35 40 45 Glu Thr cys Leu Leu Asp ile Leu Asp Thr Al a Gly Gln Glu Glu Tyr 50 55 60 Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu cys 65 70 75 80 Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu Asp Ile His Hi s Tyr 85 90 95 Arg Glu Gln Ile Lys Arg val Lys Asp Ser Glu Asp val Pro Met Val 100 105 110 Leu val Gly Asn Lys Cys Asp Leu Pro Ser Arg Thr val Asp Thr Lys 115 120 125 Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Phe lie Glu Thr 130 135 140 Ser Ala Lys Thr Arg Gln Gly val Asp Asp Al a Phe Tyr Thr Leu Val 145 150 155 160 Arg Glu lie Arg Lys His Lys Glu Lys Met Ser Lys Asp Gly Lys Lys 165 170 175 Lys Lys Lys Lys Ser Lys Thr Lys Cys val Ile Met 180 185

<210> 17 <211> 567 <212> DNA <213> Homo sapiens <400> 17 atgactgaat ataaacttgt ggtagttgga gctggtggcg taggcaagag tgccttgatg Page 10

540

567

2018219977 20 Aug 2018

118 PCT seq list atacagctaa ttcagaatca ttttgtggac gaatatgatc caacaataga ggattcctac 120 aggaagcaag tagtaattga tggagaaacc tgtctcttgg atattctcga cacagcaggt 180 caagaggagt acagtgcaat gagggaccag tacatgagga ctggggaggg ctttctttgt 240 gtatttgcca taaataatac taaatcattt gaagatattc accattatag agaacaaatt 300 aaaagagtta aggactctga agatgtacct atggtcctag taggaaataa atgtgatttg 360 ccttctagaa cagtagacac aaaacaggct caggacttag caagaagtta tggaattcct 420 tttattgaaa catcagcaaa gacaagacag ggtgttgatg atgccttcta tacattagtt 480 cgagaaattc gaaaacataa agaaaagatg agcaaagatg gtaaaaagaa gaaaaagaag 540 tcaaagacaa agtgtgtaat tatgtaa 567

<210> 18 <211> 188 <212> PRT <213> Homo sapiens <400> 18

Met 1 Thr Glu Tyr Lys 5 Leu Val val val Gly 10 Ala Gly Gly val Gly 15 Lys Ser Ala Leu Met lie Gin Leu lie Gin Asn His Phe val Asp Glu Tyr 20 25 30 Asp Pro Thr lie Glu Asp Ser Tyr Arg Lys Gin val val lie Asp Gly 35 40 45 Glu Thr cys Leu Leu Asp lie Leu Asp Thr Al a Gly Gin Glu Glu Tyr 50 55 60 Ser Ala Met Arg Asp Gin Tyr Met Arg Thr Gly Glu Gly Phe Leu cys 65 70 75 80 Val Phe Ala lie Asn Asn Thr Lys Ser Phe Glu Asp lie His His Tyr 85 90 95 Arg Glu Gin lie Lys Arg val Lys Asp Ser Glu Asp Val Pro Met val 100 105 110 Leu val Gly Asn Lys Cys Asp Leu Pro Ser Arg Thr val Asp Thr Lys 115 120 125 Gin Ala Gin Asp Leu Ala Arg Ser Tyr Gly lie Pro Phe lie Glu Thr 130 135 140 Ser Ala Lys Thr Arg Gin Gly val Asp Asp Ala Phe Tyr Thr Leu val 145 150 15 5 160 Arg Glu lie Arg Lys H1S Lys Glu Lys Met Ser Lys Asp Gly Lys Lys

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165

175

2018219977 20 Aug 2018

118 PCT seq list

170

Lys Lys Lys Lys ser Lys Thr Lys Cys Val Ile Met

180 185

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