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AU2017305399A1 - Symmetric or semi-symmetric compounds useful as immunomodulators - Google Patents

Symmetric or semi-symmetric compounds useful as immunomodulators Download PDF

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AU2017305399A1
AU2017305399A1 AU2017305399A AU2017305399A AU2017305399A1 AU 2017305399 A1 AU2017305399 A1 AU 2017305399A1 AU 2017305399 A AU2017305399 A AU 2017305399A AU 2017305399 A AU2017305399 A AU 2017305399A AU 2017305399 A1 AU2017305399 A1 AU 2017305399A1
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Prior art keywords
methyl
amino
biphenyl
methoxy
dimethyl
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AU2017305399A
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Minghua Wang
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Arising International Inc
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Arising International Inc
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Abstract

A compound having Formula I:(I), or a pharmaceutical acceptable salt thereof. A is a bivalent arene or a bivalent heteroarene; Ring B and Ring B' are independently a 6-membered aromatic hydrocarbon ring, a 6-membered heterocyclic ring, a 8- to 10-membered aromatic hydrocarbon ring, or a 8- to 10-membered heterocyclic ring; Y and Y' are independently, null (direct bond), -CHR

Description

SYMMETRIC OR SEMI-SYMMETRIC COMPOUNDS USEFUL AS IMMUNOMODULATORS
This application claims priority to US Provisional Application No. 62/370,679, filed August 3, 2016, which is incorporated by reference for all purposes as if fully set forth herein.
BACKGROUND OF THE INVENTION
Field of the Invention
The present invention generally relates to compounds useful as inhibitors of the PD1/PD-L1 protein/protein and CD80/PD-L1 protein/protein interactions.
Discussion of the Related Art
Programmed cell death-1 (PD-1, CD279) protein is a member of the CD28 superfamily that can suppress activation signals upon interaction with either of its natural ligands, program cell death-ligand 1 (PD-L1, CD274, B7-H1) and PD-L2 (CD273, B7-DC). PD-1 and its ligands are broadly expressed in immune systems and play critical roles in immune modulation including T cell activation and tolerance (Sharpe et. al., Nat. Imm. 2007). When PD-L1 expressing cells contact T cells that express PD-1, it results in attenuation of T cell activities in cytokine secretion, cytolytic activity and proliferation, in response to antigenic stimulation. Conditions of tumor progression or chronic infections could lead to chronic antigen stimulation, that results in induction of PD-1 expression and eventually reduced T cell activity (so called “T cell exhaustion”). Such suggests a biological significance of PD-1/PD-L1 interaction in attenuating infectious immunity, tumor immunity and in facilitating chronic infection and tumor progression (Keir et. al., Annu Rev Immunol. 2008). PD-L1 has also been shown to interact with CD80. The interaction between PD-L1 and CD80 on immune cells has also been shown to inhibit immune response (Deng et. al., J Immunol. 2015).
Monoclonal antibodies that block the PD-1-mediated immune checkpoint pathway can prevent T cell down regulation and promote immune responses against cancer and infectious diseases (Harvey et. al., Clin Pharmacol Ther 2014). Ample clinical and preclinical evidence has demonstrated that inhibition of PD-1/PD-L1 interaction with PD-l-or PD-L1specific antibodies can restore immune response to diverse forms of cancer and infectious diseases (Dong et. al., Nat. Med. 2002; Dong et. al., J Mol. Med. 2003). To date, several PD1/PD-L1-targeting antibodies (including pembrolizumab, nivolumab and atezolizumab) have already been approved as immuno-oncology (IO) therapeutics to treat different forms of cancer, including melanoma, non-small cell lung cancer, kidney cancer, Hodgkin lymphoma
WO 2018/026971
PCT/US2017/045185 and bladder cancer, etc. Further, studies have demonstrated that interference with PD-l/PDL1 interaction can also enhance T cell activity in chronic infection systems, thus expected to function against viral infections, like HIV (Attanasio et. al., Immunity. 2016; Porichis et. al., CurrHIV/AIDS Rep. 2012). In addition, blockage of the PD-1/PD-L1 pathway can also enhance responses to vaccination, including therapeutic vaccination in the context of viral infection (Ha et. al., J. Exp. Med. 2008). More recent discoveries also suggested therapeutic value of disrupting PD1/PD-L1 interaction for patients with certain inflammation and neurodegenerative diseases like Alzheimer’s disease (Bodhankar et. al., Stroke 2015; Baruch et. al., Nat Med. 2016).
Therefore, agents that block the interaction of PD-L1 with either PD-1 or CD80 are considered beneficial for diverse therapeutic areas, including but not limited to cancer, viral infection and vaccination. Despite recent approval of PD-1/PD-L1-targeting antibodies, there is still great demand for more potent, better selective and more easily administered therapeutics against PD-1/PD-L1 and/or CD80/PD-L1 protein-protein interactions. Recently, the elucidation of structural details of the human PD-1/PD-L1 complex has provided unique insights into the molecular mechanism of PD-1/PD-L1 protein-protein interaction (Zak et. al., Oncotarget 2016; Zak et. al., Structure 2015). Such has afforded an opportunity for structuralbased design of small molecule inhibitors to target PD-1/PD-L1 and/or CD80/PD-L1 interaction.
In this invention, applicant discovered potent small molecules that can have activity as inhibitors of the interaction of PD-L1 with PD-1 and/or CD80, and thus may be useful for therapeutic administration to enhance immunity against cancer and/or infectious diseases. These small molecules are expected to be useful as pharmaceuticals with desirable stability, bioavailability, therapeutic index, and toxicity values that are crucial to become efficient medicines to promote human health.
SUMMARY OF THE INVENTION
The inhibitors of the PD-1/PD-L1 protein/protein and CD80/PD-L1 protein/protein
Figure AU2017305399A1_D0001
interactions are a compound having Formula I:
(I), or a pharmaceutical acceptable salt thereof. A is a bivalent arene or a bivalent heteroarene; Ring B and Ring B’ are independently a 6-membered aromatic hydrocarbon
WO 2018/026971
PCT/US2017/045185 ring, a 6-membered heterocyclic ring, a 9- to 10-membered aromatic hydrocarbon ring, or a 9- to 10-membered heterocyclic ring; Y and Y’ are independently, null (direct bond), -CHRi-, -CH2-CH2-, -NR1-, -0-, -0CH2-, -CH20-, -SCH2-, -CH2S-, -SOCH2-, -CH2SO- or -SO2CH2-, and Ri is H, Ci-6 alkyl, or C3-6 cycloalkyl; R3 and R’3 are independently H, SO2NH2,
SO2NR5R6, SO2NHR7, ''' OH , / 0CHs , CH2NR5R6, or CH2NHR7, wherein R5 and Re are independently H, Ci-6 alkyl, C3.8 cycloalkyl, or heteroaryl or R5 and Rr, form a C3-8 cycloalkyl, heterocyclyl, or heteroaryl ring, and R7 is H, aryl, heteroaryl, acetyl, CH2CH2OH, CH2CH2NHCOCH3, C3-C8 alkyl carboxylic acid, C3-C8 alkyl amide, C3-C8 alkyl alcohol, CH2-Ar, or -CH2-heterocyclyl; and R4, R’4, Z, and Z’ are independently H, halogen, CHF2, CF3, CN, Ci_6 alkyl, Ci-6 alkoxy, aryl, or heteroaryl.
Further described is an isomer or tautomer thereof, a pharmaceutical acceptable solvate thereof, or a pharmaceutical acceptable prodrug thereof.
In one aspect, the Formula (I) is represented by following Formula (II):
R3\ /Χ2χ /X’2^ /R’3
Ί[©ι’
r4 Y Y r4
z ζ· (II). Ring Β and Ring Β’ are independently a 6-
membered aromatic hydrocarbon ring or a 6-membered heterocyclic ring; Xi, X’i, X2, and X’2 are independently CR2, C=O or NR2, and R2 is H, Me, CN, halogen, OMe, CHF2, CF3, Ci_6 alkoxyl, C3.7 cycloalkyl, C3.7 heterocyclyl, or OCH2Ar; and X3, and X’3 are independently C or N. Y and Y’ are independently -CHRi-, -CH2-CH2-, -NRi-, -0-, -0CH2-,
-CH20-, -SCH2-, -CH2S-, -SOCH2-, -CH2SO- or -SO2CH2-, and Ri is H, Ci_6 alkyl, or C3.6 cycloalkyl;
In one aspect, the Formula (I) is represented by following Formula (III):
Figure AU2017305399A1_D0002
z’ (III). Ring B and Ring B’are independently a 9- to 10-membered aromatic hydrocarbon ring or a 9- to 10-membered heterocyclic ring;Xi, and X’i are independently CR2, C=O or NR2, and R2 is H, Me, CN, halogen, OMe, CHF2, CF3, Ci-6 alkoxyl, C3.7 cycloalkyl, C3.7 heterocyclyl, or OCH2Ar; X2, X’2; X3, and X’3 are independently C or N; U and U’ are independently C and N; V and V’
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PCT/US2017/045185 are O, S, -(CH2)2-, -CR’-, or -N=; and Y and Y’ are independently, null (direct bond), -CHRi, -CH2-CH2-, -NR1-, -0-, -OCH2-, -CH2O-, -SCH2-, -CH2S-, -SOCH2-, -CH2SO- or -SO2CH2, and Ri is H, Ci-6 alkyl, or C3-6 cycloalkyl;
Figure AU2017305399A1_D0003
and Ra’ are independently COR’, OMe, halogen, Ci-6 alkyl, C2_6alkynyl, Ci-6 cycloalkyl, CN, CF3; CH2CF3; R’ is Ci-6 alkyl; Rb and Rb’ are independently Ci-6 alkyl, C2_6 alkynyl, Ci-6 cycloalkyl or alkyl halogen, and V is -(CH2)2-, -CR’-, or -N=,wherein Y and Y’ are independently in each occurrence -NRi-, -0-, -0CH2-, -SCH2-, -SOCH2-, or -SO2CH2-, provided that the nitrogen of ring A cannot be the connecting atom with Y and Y’.
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PCT/US2017/045185
Figure AU2017305399A1_D0004
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PCT/US2017/045185
Figure AU2017305399A1_D0005
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PCT/US2017/045185 (direct bond), -NRi-, -0-, -CH2O-, or -CH2S, provided that U in ring B and U’ in ring B’ cannot be nitrogen.
In one aspect, in Formula (I), R3 and R’3 are the same, R4 and R’4 are the same, Y and Y’ are the same, Z and Z’ are the same, or Ring B and Ring B’ are the same.
In one aspect, in Formula (I), R3 and R’3 are the same, R4 and R’4 are the same, Y and Y’ are the same, Z and Z’ are the same, and Ring B and Ring B’ are the same.
In one aspect, the inhibitors of the PD-1/PD-L1 protein/protein and CD80/PD-L1 protein/protein interactions are 2-({[4-({3'-[(4-{[(2-hydroxyethyl)amino]methyl}-2methylphenoxy)methyl]-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -yl }methoxy)-3 methylphenyl]methyl)amino)ethan-l-ol, 2-{[(4-{[3-({4-[(azetidin-l-yl)methyl]-2methylphenoxy ) methyl)-2-chlorophenyl]methoxy )-3 -methylphenyl)m ethyl] amino ) ethan-1 ol, l-[(4-{[3-({4-[(azetidin-l-yl)methyl]-2-methylphenoxy}methyl)-2chlorophenyl]methoxy)-3-methylphenyl)methyl]azetidine, (2S)-l-[[4-({3'-[(4-{[(2S)-2carboxypiperidin-l-yl]methyl)-3,5-dimethoxyphenoxy)methyl]-2,2'-dimethyl-[l,l'biphenyl]-3-yl)methoxy)-2,6-dimethoxyphenyl]methyl)piperidine-2-carboxylic acid, (2S)-1{[4-({3'-[(4-{[(2-hydroxyethyl)amino]methyl)-3,5-dimethoxyphenoxy)methyl]-2,2'dimethyl-) l,l'-biphenyl]-3-yl}methoxy)-2,6-dimethoxyphenyl]methyl)piperidine-2carboxylic acid, 2-({[4-({3[(4-{[(2-hydroxyethyl)amino]methyl )-3,5dimethoxyphenoxy)methyl]-2,2'-dimethyl-[l,l'-biphenyl]-3-yl}methoxy)-2,6dimethoxyphenyl]methyl}amino)ethan-l-ol, l-{[4-({3'-[(4-{[(2hy droxy ethyl)amino]methyl)-2-methylphenoxy)methyl]-2,2'-dimethyl-[l,l'-biphenyl]-3yl)methoxy)-2-methoxyphenyl]methyl)azetidin-3-ol, 2-{[(6-{ [3-(5-( [(5-{ [(2hy droxy ethyl)amino]methyl}-6-methoxypyridin-2-yl)oxy]methyl)-4-methylthiophen-3-yl)-2methylphenyl]methoxy}-2-methoxypyridin-3-yl)methyl]amino)ethan-l-ol, 2-(( [4-((4-((4{[(2-hydroxyethyl)amino]methyl]-3-methoxyphenoxy) methyl]-3-methylthiophen-2yl }methoxy)-2-methoxyphenyl]methyl}amino)ethan-1 -ol, 2-({4-[(azetidin-1 -yl)methyl]-2methylphenoxy }methyl)-6-{ [4-({ [(4-oxoazetidin-2yl)methyl]amino)methyl)phenoxy]methyl}benzonitrile, N-(2-([(4-([3-({4-[(azetidin-lyl)methyl]-2-methylphenoxy}methyl)-2-cyanophenyl]methoxy) phenyl)methyl]amino)ethyl)acetamide, 2-({4-[(azetidin-l-yl)methyl]-2methylphenoxy)methyl)-6-[(4-{[(2hy droxy ethyl)amino]methyl (phenoxy )methyl]benzonitrile, 2-({4-[(azetidin-l-yl)methyl]-2methylphenoxy) methyl)-6-({4-[(azetidin-l-yl)methyl]phenoxy) methyl)benzonitrile, 2-(((4((4-((4-( [(2-hydroxy ethyl)amino]methyl )-3,5-dimethoxyphenoxy (methyl]-lH-indol-1
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PCT/US2017/045185 yl }methyl)phenyl]methyl}amino)ethan-1 -ol, 2-({ [4-({1 -[(4-{[(2hydroxyethyl)amino]methyl)phenyl)methyl]-lH-indazol-4-yl)methoxy)-2,6dimethoxyphenyl]methyl)amino)ethan-l-ol, 2,6-bis[(4-{[(2-hydroxyethyl)amino]methyl}-3methoxyphenoxy)methyl]benzonitrile, N-[(4-[[3'-({4-[(cyclopropylamino)methyl]-3,5dimethoxyphenoxy}methyl)-2,2'-dimethyl-[l,r-biphenyl]-3-yl]methoxy )-2,6dimethoxyphenyl)methyl]cyclopropanamine, 4-{[({4-[(3'-{[3,5-dimethoxy-4-({[(4oxoazetidin-2-yl)methyl]amino)methyl)phenoxy]methyl)-2,2'-dimethyl-[l,r-biphenyl]-3yl)methoxy]-2,6-dimethoxyphenyl)methyl)amino]methyl)azetidin-2-one, 2-[({6-[(3'-{[(5{[(2-hydroxyethyl)amino]methyl)pyridin-2-yl)oxy]methyl)-2,2'-dimethyl-[l,r-biphenyl]-3yl)methoxy]pyridin-3-yl)methyl)amino]ethan-l-ol, 2-[({6-[(3'-{[(5-{[(2hydroxyethyl)amino]methyl} -6-methoxypyridin-2-yl)oxy]methyl} -2,2'-dimethyl-[ 1, Γbiphenyl]-3-yl)methoxy]-2-methoxypyridin-3-yl}methyl)amino]ethan-l-ol, N-{2-[({6-[(3'{[(5-{[(2-acetamidoethyl)amino]methyl}-6-methoxypyridin-2-yl)oxy]methyl}-2,2'-dimethyl[1,1 '-biphenyl]-3 -yl)methoxy]-2-methoxypyridin-3 -yl }methyl)amino] ethyl} acetamide, (2R,4R)-l-({6-[(3'-{[(5-{[(2R,4R)-2-carboxy-4-hydroxypyrrolidin-l-yl]methyl}-6methoxypyridin-2-yl)oxy]methyl} -2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -yl)m ethoxy]-2methoxypyridin-3-yl)methyl)-4-hydroxypyrrolidine-2-carboxylic acid, (2R,4R)-4-hydroxy-l({6-[(3'-{[(5-{[(2-hydroxyethyl)amino]methyl)-6-methoxypyridin-2-yl)oxy]methyl}-2,2'dimethyl-[l,r-biphenyl]-3-yl)methoxy]-2-methoxypyridin-3-yl)methyl)pyrrolidine-2carboxylic acid, (3R)-l-{[4-({3'-[(4-{[(3R)-3-hydroxypyrrolidin-l-yl]methyl}-2methylphenoxy)methyl]-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -yl }methoxy)-3 methylphenyl]methyl)pyrrolidin-3-ol, (2R,4R)-l-{[4-({3'-[(4-{[(2R,4R)-2-carboxy-4hydroxypyrrolidin-1 -yl]methyl} -2-methylphenoxy)methyl]-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 yl)methoxy)-3-methylphenyl]methyl)-4-hydroxypyrrolidine-2-carboxylic acid, 2-({[4-({3'[(4-{[(2-hydroxyethyl)amino]methyl]-3,5-dimethoxyphenoxy)methyl]-2'-methyl-[l,l·biphenyl]-3-yl)methoxy)-2,6-dimethoxyphenyl]methyl)amino)ethan-l-ol, 2-[({4-[(2-{3-[(4{[(2-hydroxyethyl)amino]methyl)-3,5-dimethoxyphenoxy)methyl]-2-methylphenyl)pyridin4-yl)methoxy]-2,6-dimethoxyphenyl)methyl)amino]ethan-l-ol, 2-[({4-[(6-{3-[(4-{[(2hydroxyethyl)amino]methyl)-3,5-dimethoxyphenoxy)methyl]-2-methylphenyl)pyridin-2yl)methoxy]-2,6-dimethoxyphenyl)methyl)amino]ethan-l-ol, 2-({[4-({2'-chloro-3'-[(4-{[(2hydroxyethyl)amino]methyl} -2-methylphenoxy)methyl]-[ 1,1 '-biphenyl]-3 -yl }methoxy)-3 methylphenyl]methyl)amino)ethan-l-ol, 3,3'-bis[(4-{[(2-hydroxyethyl)amino]methyl}-3,5dimethoxyphenoxy)methyl]-[l,r-biphenyl]-2-carbonitrile, 3,3'-bis({[(5-{[(2hydroxyethyl)amino]methyl }-6-methoxypyridin-2-yl)oxy]methyl})-[ 1,1 '-biphenyl]-2
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PCT/US2017/045185 carbonitrile, 3,3'-bis(([(5-([(2-hydroxyethyl)amino]methyl)pyridin-2-yl)oxy]methyl))-[l,l·biphenyl]-2-carbonitrile, 3,3'-bis({4-[(azetidin-l-yl)methyl]-3,5-dimethoxyphenoxy)methyl)[l,r-biphenyl]-2-carbonitrile, 3,3'-bis((4-[(3-hydroxyazetidin-l-yl)methyl]-3,5dimethoxyphenoxy}methyl)-[l,r-biphenyl]-2-carbonitrile, 3,3'-bis(([3,5-dimethoxy-4-({[(4oxoazetidin-2-yl)methyl]amino}methyl)phenoxy]methyl})-[l,r-biphenyl]-2-carbonitrile, 3,3'-bis[(4-( [(2-hy droxy ethyl)amino]methyl)-2-methylphenoxy)methyl]-[l,l'-biphenyl]-2carbonitrile, N-[2-[({6-[(2'-cyano-3'-{[(5-{[(2-acetamidoethyl)amino]methyl}-6methoxypyridin-2-yl)oxy]methyl}-[l,r-biphenyl]-3-yl)methoxy]-2-methoxypyridin-3yl}methyl)amino]ethyl}acetamide, 2-((4-( [(2-hydroxyethyl)amino]methyl)-3,5dimethoxyphenoxy)methyl]-6-[4-[(4-{[(2-hydroxyethyl)amino]methyl )-3,5dimethoxyphenoxy)methyl]pyridin-2-yl (benzonitrile, 2-((4-( [(2hydroxyethyl)amino]methyl(-2-methylphenoxy)methyl]-6-{4-[(4-{[(2hydroxyethyl)amino]methyl(-2-methylphenoxy)methyl]pyridin-2-yl(benzonitrile, 2-(((5{((2-hy droxy ethyl)amino]methyl)-6-methoxypyridin-2-yl)oxy]methyl )-6-(4-( [(5-( [(2hydroxyethyl)amino]methyl(-6-methoxypyridin-2-yl)oxy]methyl(pyridin-2-yl)benzonitrile,
2-[({4-[(2-{3-[(4-{[(2-hydroxyethyl)amino]methyl)-2-methylphenoxy)methyl]-2methylphenyl}pyridin-4-yl)methoxy]-3-methylphenyl)methyl)amino]ethan-l-ol, 3,3'bis({[(5-{[(2-hydroxyethyl)amino]methyl)pyridin-2-yl)oxy]methyl})-[l,r-biphenyl]-2,2'dicarbonitrile, 3,3'-bis[(4-{[(2-hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-[l,l·biphenyl]-2,2'-dicarbonitrile, 3,3'-bis[(4-{[(2-hydroxyethyl)amino]methyl)phenoxy)methyl][l,r-biphenyl]-2,2'-dicarbonitrile, 5,5'-bis[(4-{[(2-hydroxyethyl)amino]methyl}-2methylphenoxy)methyl]-[3,3'-bipyridine]-4,4'-dicarbonitrile, 2-(((4-((4-(3-((4-(((2hy droxy ethyl)amino]methyl} -3,5 -dimethoxyphenoxy (methyl] -2-methylphenyl )-3methylthiophen-2-yl)methoxy]-2,6-dimethoxyphenyl)methyl)amino]ethan-l-ol, 2-(((4-((4{ 3 - [(4- {[(2-hy droxy ethyl)amino]methyl) -2-methylphenoxy (methyl] -2-methylphenyl )-3methylthiophen-2-yl)methoxy]-3-methylphenyl)methyl)amino]ethan-l-ol, 2-(( [4-((5'-[(4{[(2-hy droxy ethyl)amino]methyl)-2-methylphenoxy)methyl]-4,4'-dimethyl-[3,3'bithiophene]-5-yl)methoxy)-3-methylphenyl]methyl)amino)ethan-l-ol, 2-(([4-((5'-[(4-([(2hy droxy ethyl)amino]methyl) -2-methylphenoxy)methyl]-1,1 '-dimethyl- 1H, 1 Ή-[2,2'bipyrrole]-5-yl)methoxy)-3-methylphenyl]methyl)amino)ethan-l-ol, 5-((5-([3'-((5-[(5cy anopyridin-3-yl)methoxy]-4-{ [(2-hy droxy ethyl)amino]methyl)-2-methylphenoxy (methyl)2,2'-dimethyl-[l,l'-biphenyl]-3-yl]methoxy )-2-( [(2-hy droxy ethyl)amino]methyl )-4methylphenoxy)methyl]pyridine-3-carbonitrile, 2-(([4-((3'-[(4-([(2hydroxyethyl)amino]methyl)-2-methyl-5-[(pyridin-3-yl)methoxy]phenoxy)methyl]-2,2'
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PCT/US2017/045185 dimethyl-[l,r-biphenyl]-3-yl]methoxy)-5-methyl-2-[(pyridin-3yl)methoxy]phenyl]methyl}amino)ethan-l-ol, 5-[(4-chloro-5-{[3'-({2-chloro-5-[(5cyanopyridin-3-yl)methoxy]-4-{[(2-hydroxyethyl)amino]methyl}phenoxy}methyl)-2,2'dimethyl-[l,l'-biphenyl]-3-yl]methoxy}-2-{[(2hydroxyethyl)amino]methyl}phenoxy)methyl]pyridine-3-carbonitrile, 5-[(5-{[3'-({5-[(5cyanopyridin-3-yl)methoxy]-2-methyl-4-[(methylamino)methyl]phenoxy}methyl)-2,2'dimethyl-[l,l'-biphenyl]-3-yl]methoxy}-4-methyl-2[(methylamino)methyl]phenoxy)methyl]pyridine-3-carbonitrile, 5-[(5-{[3'-({5-[(5cyanopyridin-3-yl)methoxy]-4-(hydroxymethyl)-2-methylphenoxy}methyl)-2,2'-dimethyl[l,r-biphenyl]-3-yl]methoxy}-2-(hydroxymethyl)-4-methylphenoxy)methyl]pyridine-3carbonitrile, 5-[(3-{[3'-({5-[(5-cyanopyridin-3-yl)methoxy]-2-methylphenoxy}methyl)-2,2'dimethyl-[l,r-biphenyl]-3-yl]methoxy}-4-methylphenoxy)methyl]pyridine-3-carbonitrile, 5[(5-{[3'-({5-[(5-cyanopyridin-3-yl)methoxy]-4-(hydroxymethyl)-2-methylphenoxy}methyl)-
2-methyl-[l,l'-biphenyl]-3-yl]methoxy}-2-(hydroxymethyl)-4methylphenoxy)methyl]pyridine-3-carbonitrile, 3,3'-bis({5-[(5-cyanopyridin-3-yl)methoxy]4-{[(2-hydroxyethyl)amino]methyl}-2-methylphenoxy}methyl)-[l,r-biphenyl]-2,2'dicarbonitrile, 5-({4-chloro-5-[(2-cyano-3'-{[(5-{[(2-hydroxyethyl)amino]methyl}pyridin-2yl)oxy ]methyl} - [ 1,1 '-biphenyl] -3 -yl)methoxy ] -2- {[(2hydroxyethyl)amino]methyl}phenoxy}methyl)pyridine-3-carbonitrile, 5-{[5-({2-cyano-3'[(4-{[(2-hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-[l,r-biphenyl]-3yl}methoxy)-2-{[(2-hydroxyethyl)amino]methyl}phenoxy]methyl}pyridine-3-carbonitrile, 5{[4-chloro-5-({2-cyano-3'-[(4-{ [(2-hydroxy ethyl)amino]methyl}-2-methylphenoxy)methyl][1,1 '-biphenyl]-3 -yl }methoxy)-2- {[(2hydroxyethyl)amino]methyl}phenoxy]methyl}pyridine-3-carbonitrile, 3'-[(4-{ [(2hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-3-[(4-{[(2hydroxyethyl)amino]methyl}-3-[(pyridin-3-yl)methoxy]phenoxy)methyl]-[l,r-biphenyl]-2carbonitrile, 5-{[5-({2'-cyano-3'-[(4-[[(2-hydroxyethyl)amino]methyl}-2methylphenoxy)methyl]-[ 1,1 '-biphenyl]-3-yl }methoxy)-2-{ [(2-hydroxy ethyl)amino]methyl }4-methylphenoxy]methyl}pyridine-3-carbonitrile, 5-{[5-({2-cyano-3'-[(4-{[(2hydroxyethyl)amino]methyl} -2-methylphenoxy)methyl]-[ 1,1 '-biphenyl]-3 -yl}methoxy)-2{[(2-hydroxyethyl)amino]methyl}-4-methylphenoxy]methyl}pyridine-3-carbonitrile, 5-[(4chl oro-2- {[(2-hy droxy ethyl)amino]methyl }-5-[(3'-{[(5-{[(2hydroxyethyl)amino]methyl}pyridin-2-yl)oxy]methyl}-2,2'-dimethyl-[l,r-biphenyl]-3yl)methoxy]phenoxy)methyl]pyridine-3-carbonitrile, 5-({4-chloro-5-[(2-cyano-3'-{[(5-{[(2
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PCT/US2017/045185 hydroxy ethyl)amino]methyl }pyridin-2-yl)oxy]methyl}-[1,1 '-biphenyl]-3 -yl)methoxy]-2[(cyclopropylamino)methyl]phenoxy}methyl)pyridine-3-carbonitrile, 5-{[5-({2-cyano-3'-[(4{[(2-hy droxy ethyl)amino]methyl}-3,5-dimethoxyphenoxy)methyl]-2'-methyl-[l,r-biphenyl]-
3-yl }methoxy)-2-{ [(2-hydroxy ethyl)amino]methyl}-4-methylphenoxy]methyl]pyridine-3carbonitrile, 5-[(5-{[2-cyano-3'-([5-[(5-cyanopyridin-3-yl)methoxy]-4-{[(2hydroxyethyl)amino]methyl}-2-methylphenoxy}methyl)-[l,r-biphenyl]-3-yl]methoxy}-2{[(2-hydroxyethyl)amino]methyl}-4-methylphenoxy)methyl]pyridine-3-carbonitrile, 2-({[4({3'-[(4-{ [(2-hydroxy ethyl)amino]methyl}-3-[(pyridin-3-yl)methoxy]phenoxy)methyl]-2,2'dimethyl-[ 1,1 '-biphenyl]-3-yl }methoxy)-3-methylphenyl]methyl}amino)ethan-1 -ol, 5-[(4chloro-2-{[(2-hydroxyethyl)amino]methyl}-5-({3'-[(4-{[(2-hydroxyethyl)amino]methyl}-3,5dimethoxyphenoxy)methyl]-2-methyl-[l,r-biphenyl]-3yl}methoxy)phenoxy)methyl]pyridine-3-carbonitrile, 5-[(2-{[(2hy droxy ethyl)amino]methyl}-5-({3'-[(4-{ [(2-hy droxy ethyl)amino]methyl}-2methylphenoxy)methyl]-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -yl }methoxy)-4methylphenoxy)methyl]pyridine-3-carbonitrile, 5-[(2-{[(2-hydroxyethyl)amino]methyl}-5({3'-[(4-{[(2-hydroxyethyl)amino]methyl}-3,5-dimethoxyphenoxy)methyl]-2,2'-dimethyl[l,r-biphenyl]-3-yl}methoxy)-4-methylphenoxy)methyl]pyridine-3-carbonitrile, 2-({[4-({3'[(4-{[(2-hy droxy ethyl)amino]methyl}-2-methyl-5-[(pyridin-3-yl)methoxy]phenoxy)methyl]2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -yl }methoxy)-2,6-dimethoxyphenyl]methyl} amino)ethan-1 -ol, 5 - {[(3 - {[(2-hy droxy ethyl)amino]methyl} -6- [(3{[(5 - {[(2-hydroxy ethyl)amino]methyl }-6methoxypyridin-2-yl)oxy]methyl}-2,2'-dimethyl-[l,r-biphenyl]-3-yl)methoxy]pyridin-2yl)oxy]methyl}pyridine-3-carbonitrile, 2-({ [4-({3'-[(2-chloro-4-{ [(2- hy droxy ethyl)amino]methyl}-5-[(pyridin-3-yl)methoxy]phenoxy)methyl]-2,2'-dimethyl-[ 1,1'biphenyl]-3-yl}methoxy)-3-methylphenyl]methyl}amino)ethan-l-ol, 5-[(5-{[2-cyano-3'-({5[(5-cyanopyridin-3-yl)methoxy]-4-(hydroxymethyl)-2-methylphenoxy}methyl)-2'-methyl[l,r-biphenyl]-3-yl]methoxy}-2-(hydroxymethyl)-4-methylphenoxy)methyl]pyridine-3carbonitrile, 5-({2-[(azetidin-l-yl)methyl]-5-[[3'-({4-[(azetidin-l-yl)methyl]-5-[(5cyanopyridin-3-yl)methoxy]-2-methylphenoxy}methyl)-2-cyano-[l,r-biphenyl]-3yl]methoxy}-4-methylphenoxy}methyl)pyridine-3-carbonitrile, 5-({2-[(azetidin-lyl)methyl]-5-{[3'-({4-[(azetidin-l-yl)methyl]-5-[(5-cyanopyridin-3-yl)methoxy]-2methylphenoxy}methyl)-2,2'-dimethyl-[l,r-biphenyl]-3-yl]methoxy}-4methylphenoxy}methyl)pyridine-3-carbonitrile, 2-[({2-[3'-(6-{[(2hy droxy ethyl)amino]methyl} -[ 1,2,4]tri azolof 1,5-a]pyridin-2-yl)-2,2'-dimethyl-[ 1 ,Γbiphenyl]-3-yl]-[l,2,4]triazolo[l,5-a]pyridin-6-yl}methyl)amino]ethan-l-ol, 2-[({2-[3'-(6
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PCT/US2017/045185 {[ (2-hy droxy ethyl)amino]methyl }imidazo[ 1,2-a]pyridin-2-yl)-2,2'-dimethyl-[ 1,1 '-biphenyl]-
3-yl]imidazo[l,2-a]pyridin-6-yl}methyl)amino]ethan-l-ol, 2-[({2-[3'-(6-{[(2hydroxyethyl)amino]methyl}-l,3-benzoxazol-2-yl)-2,2'-dimethyl-[l,r-biphenyl]-3-yl]-l,3benzoxazol-6-yl}methyl)amino]ethan-l-ol, 2-({2-[3'-(5-{[(2-hydroxyethyl)amino]methyl}-
1,3 -benzoxazol-2-yl)-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -y 1 ] -1,3 -benzoxazol-5-yl} amino)ethan-
1- ol, 2-[({2-[3'-(5-{[(2-hydroxyethyl)amino]methyl}-l,3-benzothiazol-2-yl)-2,2'-dimethyl[l,r-biphenyl]-3-yl]-l,3-benzothiazol-5-yl}methyl)amino]ethan-l-ol, 2-[({2-[3'-(6-{[(2- hy droxy ethyl)amino]methyl}-l,3-benzothiazol-2-yl)-2,2'-dimethyl-[l,r-biphenyl]-3-yl]-1,3benzothiazol-6-yl}methyl)amino]ethan-l-ol, 3,3'-bis(6-{[(2-hydroxyethyl)amino]methyl}- [1.2.4] triazolo[l,5-a]pyridin-2-yl)-[l,r-biphenyl]-2,2'-dicarbonitrile, 3,3'-bis(6-{[(2hydroxyethyl)amino]methyl}-l,3-benzoxazol-2-yl)-[l,r-biphenyl]-2,2'-dicarbonitrile, 2-(((2[3'-(7-{[(2-hydroxyethyl)amino]methyl}-[l,2,4]triazolo[l,5-a]pyridin-2-yl)-2,2'-dimethyl[l,r-biphenyl]-3-yl]-[l,2,4]triazolo[l,5-a]pyridin-7-yl}methyl)amino]ethan-l-ol, 2-{[(8- chl oro-2-{3'-[(4-{ [(2-hy droxy ethyl)amino]methyl}-2-methylphenoxy)methyl]-2,2'-dimethyl[1,1 '-biphenyl]-3-yl}-[ 1,2,4]triazolo[ 1,5-a]pyridin-6-yl)methyl]amino} ethan-1 -ol, 3-(8chloro-6-{[(2-hydroxyethyl)amino]methyl}-[l,2,4]triazolo[l,5-a]pyridin-2-yl)-3'-[(4-{[(2hy droxy ethyl)amino]methyl}-2-methylphenoxy)methyl]-[l,r-biphenyl]-2-carbonitrile, 3'[(4- {[(2-hy droxy ethyl)amino]methyl} -3,5 -dimethoxyphenoxy)methyl] -3 -(6- {[(2hydroxyethyl)amino]methyl} -[ 1,2,4]triazolo[ 1,5-a]pyridin-2-yl)-[ 1,1 '-biphenyl]-2carbonitrile, 3'-[(4-{ [(2-hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-3-(6-{ [(2hy droxy ethyl)amino]methyl} -[ 1,2,4]triazolo[ 1,5-a]pyridin-2-yl)-[ 1,1 '-biphenyl]-2carbonitrile, 2-{[(2-{3'-[(4-{[(2-hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-2,2'dimethyl-[l,r-biphenyl]-3-yl}-l,3-benzoxazol-6-yl)methyl]amino}ethan-l-ol, 3-(5-{[(2hy droxy ethyl)amino]methyl}-l,3-benzoxazol-2-yl)-3'-[(4-{ [(2-hy droxy ethyl)amino]methyl}-
2- methylphenoxy)methyl]-[l,r-biphenyl]-2-carbonitrile, 2-{[(2-{3'-[(4-{[(2- hy droxy ethyl)amino]methyl}-2-methylphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}-
1.3- benzoxazol-5-yl)methyl]amino}ethan-l-ol, 2-{[(2-{3'-[(4-{[(2- hy droxy ethyl)amino]methyl}-2-methylphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}-
1.3- benzothiazol-5-yl)methyl]amino}ethan-l-ol, 2-{[(2-{3'-[(4-{[(2- hy droxy ethyl)amino]methyl}-2-methylphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}-
1.3- benzothiazol-6-yl)methyl]amino}ethan-l-ol, 2-{[(2-{3'-[(4-{[(2- hy droxy ethyl)amino]methyl}-2-methylphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}- [1.2.4] triazolo[l,5-a]pyridin-6-yl)methyl]amino}ethan-l-ol, 3'-[(4-{[(2hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-3-(6-{[(2
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3-yl]imidazo[ 1,2-b] [ 1,2,4]triazin-2-yl )methyl)amino]ethan-1 -ol, 2-[({2-[3'-(6-{[(2hy droxy ethyl)amino]methyl )imidazo[ 1,2-a]pyrazin-2-yl)-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 yl]imidazo[l,2-a]pyrazin-6-yl)methyl)amino]ethan-l-ol, 2-({[4-({3-[2-(4-{[(2hy droxy ethyl)amino]methyl )phenyl)-7-methyl-l,3-benzoxazol-6-yl]-2m ethylphenyl) methoxy)-3 -methylphenyl Jmethyl) amino)ethan- l-ol,2-({[4-({3-[2-(3-{[(2hy droxy ethyl)amino]methyl )phenyl)-7-methyl-l,3-benzoxazol-6-yl]-2m ethylphenyl) methoxy)-3 -methylphenyl jmethyl) amino)ethan- 1 -ol, 2- {[(4- { 6- [2-(4- {[(2hy droxy ethyl)amino]methyl)phenyl)-7-methyl-l,3-benzoxazol-6-yl]-7-methyl-1,3benzoxazol-2-yl) phenyl)methyl] amino ) ethan- 1 -ol, 2-{[(3-{6-[2-(3-{[(2hy droxy ethyl)amino]methyl)phenyl)-7-methyl-l,3-benzoxazol-6-yl]-7-methyl-1,3benzoxazol-2-yl)phenyl)methyl]amino)ethan-l-ol, 2-[({2-[3'-(5-{[(2hy droxy ethyl)amino]methyl )-2,3-dihydro-lH-isoindol-2-yl)-2,2'-dimethyl-[l,r-biphenyl]-3yl]-2,3-dihydro-lH-isoindol-5-yl)methyl)amino]ethan-l-ol, or 2-[({6-[3'-(3-{[(2hydroxyethyl)amino]methyl)-2-methoxy-5H,6H,7H-pyrrolo[3,4-b]pyridin-6-yl)-2,2'dimethyl-[l,r-biphenyl]-3-yl]-2-methoxy-5H,6H,7H-pyrrolo[3,4-b]pyridin-3yl)methyl)amino] ethan- l-ol.
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In another aspect, described herein is a pharmaceutical composition comprising the compound of Formula (I) or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
In another aspect, described herein is a method of treating a disease associated with the modulation of PD-1/PD-L1 or CD80/PD-L1 interaction comprising administering to a patient the compound of Formula (I) or a pharmaceutically acceptable salt thereof.
In another aspect, in the method, the disease is an infection, inflammation cancer, or neurodegenerative disorders like Alzheimer’s disease.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to provide further explanation of the invention as claimed.
BRIEF DESCRIPTION OF THE DRAWINGS
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention.
In the drawings:
Figure 1 shows the docking pose of compound 1-1 in PD-L1 dimer.
Figure 2 shows the docking pose of compound I-10 in PD-L1 dimer.
DETAILED DESCRIPTION OF THE ILLUSTRATED EMBODIMENTS
Reference will now be made in detail to embodiments of the present invention, example of which is illustrated in the accompanying drawings.
Prodrugs mean any compound which releases an active parent drug according to Formula (I) in vivo when such prodrug is administered to a mammalian subject. Prodrugs of a compound of Formula (I) are prepared by modifying functional groups present in the compound of Formula (I) in such a way that the modifications may be cleaved in vivo to release the parent compound. Prodrugs may be prepared by modifying functional groups present in the compounds in such a way that the modifications are cleaved, either in routine manipulation or in vivo, to the parent compounds.
Tautomers mean compounds produced by the phenomenon wherein a proton of one atom of a molecule shifts to another atom. Tautomers also refer to one of two or more structural isomers that exist in equilibrium and are readily converted from one isomeric form to another. One of ordinary skill in the art would recognize that other tautomeric ring atom
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Isomers mean compounds having identical molecular formulae but differ in the nature or sequence of bonding of their atoms or in the arrangement of their atoms in space. Isomers that differ in the arrangement of their atoms in space are termed stereoisomers. Stereoisomers that are not mirror images of one another are termed diastereomers, and those that are nonsuperimposable mirror images of each other are termed enantiomers. When a compound has an asymmetric center, for example, it is bonded to four different groups, a pair of enantiomers is possible. A chiral compound can exist as either individual enantiomer or as a mixture thereof. Unless otherwise indicated, the description is intended to include individual stereoisomers as well as mixtures.
Certain compounds of the present disclosure can exist in unsolvated forms as well as solvated forms, including hydrated forms. Solvates refer to a complex formed by combination of solvent molecules with the compound of Formula (I). The solvent can be an organic compound, an inorganic compound, or a mixture thereof.
Pharmaceutically acceptable salts represent those salts which are, within the scope of medical judgement, suitable for use in contact for the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. They may be obtained during the final isolation and purification of the compounds of the invention, or separately by reacting the free base function with a suitable mineral acid such as hydrochloric acid, phosphoric acid, or sulfuric acid, or with an organic acid such as for example ascorbic acid, citric acid, tartaric acid, lactic acid, maleic acid, malonic acid, fumaric acid, glycolic acid, succinic acid, propionic acid, acetic acid, methanesulfonic acid, and the like. The acid function can be reacted with an organic or a mineral base, like sodium hydroxide, potassium hydroxide or lithium hydroxide.
Therapeutically effective amount means an amount of compound or a composition of the present invention effective in inhibiting the PD-1/PD-L1 protein/protein and CD80/PDL1 protein/protein interactions, and thus producing the desired therapeutic effect.
As used herein, the term alkyl refers to a monovalent straight or branched chain, saturated aliphatic hydrocarbon radical having a number of carbon atoms in the specified range. For example, Ci-6 alkyl refers to any of the hexyl alkyl and pentyl alkyl isomers as well as η-, iso-, sec- and t-butyl, n- and iso-propyl, ethyl and methyl. Alkyl also includes saturated aliphatic hydrocarbon radicals wherein one or more hydrogen atoms are replaced with deuterium, for example, CD3.
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The term branched alkyl refers to an alkyl group as defined above except that straight chain alkyl groups in the specified range are excluded. As defined herein, branched alkyl includes alkyl groups in which the alkyl is attached to the rest of the compound via a secondary or tertiary carbon. For example, isopropyl is a branched alkyl group.
The term cycloalkyl refers to any monocyclic ring of an alkane having a number of carbon atoms in the specified range. For example, C3-6cycloalkyl refers to cyclopropyl, cyclobutyl,cyclopentyl, and cyclohexyl.
The term halogen refers to fluorine, chlorine, bromine and iodine (alternatively referred to as fluoro, chloro, bromo, and iodo).
The term haloalkyl refers to an alkyl group as defined above in which one or more of the hydrogen atoms have been replaced with a halogen (i.e., F, Cl, Br and/or I). For example, Ci_6 haloalkyl refers to a Ci to C6 linear or branched alkyl group as defined above with one or more halogen substituents. The term fluoroalkyl has an analogous meaning except that the halogen substituents are restricted to fluoro. Suitable fluoroalkyls include the series (CH2)0. 4CF3.
The term C(O) or CO refers to carbonyl. The terms S(O)2 or SO2 refers to sulfonyl. The term S(O) or SO refers to sulfinyl.
The term aromatic hydrocarbons (sometimes arene or aryl hydrocarbon) refers to hydrocarbons with sigma bonds and delocalized pi electrons between carbon atoms forming rings.
The term aryl (Ar) refers to phenyl, naphthyl, tetrahydronaphthyl, idenyl, dihydroindenyl and the like. An aryl of particular interest is phenyl.
The term heterocyclic refers to heteroaryl, saturated heterocyclic, and unsaturated heterocyclic with a double bond.
The term heteroaryl refers to (i) a 5- or 6-membered heteroaromatic ring containing from 1 to 4 heteroatoms independently selected from N, O and S, or (ii) is a heterobicyclic ring selected from quinolinyl, isoquinolinyl, and quinoxalinyl. Suitable 5- and 6-membered heteroaromatic rings include, for example, pyridyl (also referred to as pyridinyl), pyrrolyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, thienyl, furanyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isooxazolyl, oxadiazolyl, oxatriazolyl, thiazolyl, isothiazolyl, and thiadiazolyl. A class of heteroaryls of interest consists of (i) 5- and 6-membered heteroaromatic rings containing from 1 to 3 heteroatoms independently selected from N, O and S, and (ii) heterobicyclic rings selected from quinolinyl, isoquinolinyl, and quinoxalinyl.
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Heteroaryls of particular interest are pyrrolyl, imidazolyl, pyridyl, pyrazinyl, quinolinyl (or quinolyl), isoquinolinyl (or isoquinolyl), and quinoxalinyl.
Examples of 4- to 7-membered, saturated heterocyclic rings within the scope of this invention include, for example, azetidinyl, piperidinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, isothiazolidinyl, oxazolidinyl, isoxazolidinyl, pyrrolidinyl, imidazolidinyl, piperazinyl, tetrahydrofuranyl, tetrahydrothienyl, pyrazolidinyl, hexahydropyrimidinyl, thiazinanyl, thiazepanyl, azepanyl, diazepanyl, tetrahydropyranyl, tetrahydrothiopyranyl, and dioxanyl. Examples of 4- to 7-membered, unsaturated heterocyclic rings within the scope of this invention include mono-unsaturated heterocyclic rings corresponding to the saturated heterocyclic rings listed in the preceding sentence in which a single bond is replaced with a double bond (e.g., a carbon-carbon single bond is replaced with a carbon-carbon double bond).
It is understood that the specific rings listed above are not a limitation on the rings which can be used in the present invention. These rings are merely representative.
Synthetic methods for preparing the compounds of the present invention are illustrated in the following Schemes, Methods, and Examples. Starting materials are commercially available or may be prepared according to procedures known in the art or as described herein. The compounds of the invention are illustrated by means of the specific examples shown below. However, these specific examples are not to be construed as forming the only genus that is considered as the invention. These examples further illustrate details for the preparation of the compounds of the present invention. Those skilled in the art will readily appreciate that known variations in the conditions and processes can be used to prepare such compounds.
Compounds of the present invention can be synthesized by following the steps outlined in General Schemes 1-7, which comprise different sequences of assembling intermediates II - XII. Starting materials are either commercially available or made by known procedures in the reported literature or as illustrated.
When A is a bivalent bi-aryl or bi-heteroaryl core, a symmetric or semi-symmetric compound of Formula (I) can be synthesized via Suzuki palladium catalyzed cross-coupling of aryl/heteroaryl halide (II) and aryl/heteroaryl boronic acid/ester (III) as shown in Scheme
1-3.
Scheme 1
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Figure AU2017305399A1_D0006
Hal = Cl, Bror I Y = CO2R, ch2oh
2. NaBH4 (Y=CO2R) orNBS (Y=CH2OH)
1. Suzuki coupling
Figure AU2017305399A1_D0007
IV
Y = CH2OH, CH2Br
Figure AU2017305399A1_D0008
Figure AU2017305399A1_D0009
R3-R4,R3’-R4’, X1-3, X’1-3, γ, Y’, z and Z’ are defined as in Formula (I).
Scheme 2
Figure AU2017305399A1_D0010
II
Hal = Cl, Bror I
Figure AU2017305399A1_D0011
Suzuki coupling
Pd cat. base, heat
Base = TEA
Figure AU2017305399A1_D0012
l-a
CS2CO3
K2CO3
III
R, R' = H, or form a cylic ester
Figure AU2017305399A1_D0013
R3—R4 R3 —Rf, X1.3, X’1.3, Y, Y’, Z and Z’ are defined as in Formula (I).
Figure AU2017305399A1_D0014
Figure AU2017305399A1_D0015
R, R' = H, or form a cylic ester
Figure AU2017305399A1_D0016
Hal = Cl, or Br
Ra
Z. / \ VHeD—\J ’“-S-V X Z
In Scheme 3, A is / Ra' ' , R3-R4. R3’-R4’, Xi-3, X’i.3, Y, Y’, Z and Z’
are defined as in Formula (I).
When A is a bi-heteroaryl core, a semi-symmetric compound of Formula (I) can be synthesized via Chan-Lam copper catalyzed C-N coupling reaction of aryl/heteroaryl boronic acid (III) with N-H containing aryl/heteroaryl (V) as shown in Scheme 3.
Scheme 4
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R.
R;
Figure AU2017305399A1_D0017
Figure AU2017305399A1_D0018
Figure AU2017305399A1_D0019
Figure AU2017305399A1_D0020
, R3-R4, R3-R4’, X1-3, X’1-3, Y, Y’, z and Z’ are defined as in Formula (I).
When A is a bivalent aryl or heteroaryl core, a symmetric or semi-symmetric compound of Formula (I) can be synthesized via Mitsunobu reaction of aryl/heteroaryl alcohol (VI) and aryl/heteroaryl phenol (VII). A compound of formula (I) can also be prepared by O-alkylation or N-alkylation of aryl/heteroaryl (VII or IX) with aryl/heteroaryl halides (VIII) under basic condition (Scheme 4 to Scheme6).
Scheme 5
Figure AU2017305399A1_D0021
VI
R = Br, OH
VII eqv.
Mitsunobu reaction or O-alkylation
R=OH, Mitsunobu reaction R= Br, O-alkylation of phenol
Figure AU2017305399A1_D0022
Symmetric compound
In Scheme 5, A is
Figure AU2017305399A1_D0023
R3-R4, R3-R4’, X1-3, X’1-3, γ, Y’, z and Z’ are defined as in Formula (I).
Scheme 6
Figure AU2017305399A1_D0024
Mitsunobu reaction or O-alkylation
Figure AU2017305399A1_D0025
NaBH4 or LAH
VIII VII
R = Br, OH
R=OH, Mitsunobu reaction R= Br, O-alkylation of phenol
Figure AU2017305399A1_D0026
Semi-symmetric compound
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In Scheme 6, A is
Ra
Figure AU2017305399A1_D0027
R3-R4, R3-R4’, X1-3, X’1-3, γ, Y’, z and Z’ are defined as in Formula (I).
Figure AU2017305399A1_D0028
I
3'
Figure AU2017305399A1_D0029
Figure AU2017305399A1_D0030
i-f
Alkylation, Base, heat
Figure AU2017305399A1_D0031
/
I
In Scheme 7, A is defined as in Formula (I).
The general ways of preparing target molecules (I) by using intermediates II - XII are outlined in Scheme 1-7. A mixture of enantiomers, diastereomers, cis/trans isomers resulted , - R3-R4, R3-R4’, X1-3, X’1-3, γ, Y’, z and Z’ are from the process can be separated into their single components by chiral salt technique, chromatography using normal phase, reverse phase or chiral column, depending on the nature of the separation.
It should be understood that in the description and formulae shown above, the various groups A, B and B’, - R3-R4, R3-R4’, X1-3, X’1-3, Y, Y’, Z and Z’ and other variables are as defined above, except where otherwise indicated. Furthermore, for synthetic purposes, the compounds of Schemes 1-7 are mere representative with elected radicals to illustrate the general synthetic methodology of the compound of Formula (I) as defined herein.
Abbreviations used in the following examples and elsewhere herein are:
Ac2O acetic anhydride
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ACN Acetonitrile
BOP ammonium 4-(3-(pyridin-3-ylmethyl)ureido)benzenesulfmate
CDCh deuterated chloroform
CS2CO3 cesium carbonate
CuSO4 copper sulfate
δ chemical shift
DCM di chloromethane or methylene chloride
DCE 1,2-di chloroethane
DEAD diethyl azodicarboxylate
DIAD diisopropyl azodicarboxylate
DIEA Λζ/V-diisopropylethylamine
DMA A; A-di methyl acetamide
DME dimethoxy ethane
DMF A; A-di methyl form am ide
DMP Dess-Martin Periodinane
DMSO dimethylsulfoxide
DMSO-t/d deuterated dimethylsulfoxide
dppf EDCI 1, l'-Bis(diphenylphosphino)ferrocene 7V-(3-dimethylaminopropyl)-7V’-ethylcarbodiimide hydrochloride
EDTA ethylenediaminetetraacetic acid
ee enantiomeric excess
EtOAc ethyl acetate
EtOH *HNMR ethanol proton nuclear magnetic resonance
HOAc acetic acid
HATU 2-(3 H-[l, 2,3]triazolo[4,5-b]pyridin-3-yl)-l, 1,3,3tetramethylisouronium hexafluorophosphate
HC1 hydrochloric acid
HOBT lH-benzo[d][ 1,2,3 ]tri azol-1 -ol hydrate
HPLC high pressure liquid chromatography
Hz hertz
IPA isopropyl alcohol
KOAc potassium acetate
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K2CO3 potassium carbonate
LAH lithium aluminum hydride
LCMS liquid chromatography/mass spectrometry
(M+l) mass + 1
m-CPBA m-chloroperbenzoic acid
MeOH methanol
MeMgBr methyl magnesium bromide
MS mass spectrometry
NaBH4 sodium borohydride
NaBH(OAc)3 sodium triacetoxyborohydride
NaBH3CN sodium cyanoborohydride
Na2SO4 sodium sulfate
NBS N-bromosuccinimide
Pd(dppf)Cl2 [l,l'-Bis(diphenylphosphino)ferrocene]dichloropalladium(II)
Palladium tetrakis Tetrakis(triphenylphosphine)palladium(0)
Rt retention time
TBAF tetrabutylammonium fluori de
TBDMS-C1 Tert-butyl dimethylsilyl chloride
TEA triethylamine
THF tetrahydrofuran
TLC thin layer chromatography
Xantphos 4,5-Bis(diphenylphosphino)-9,9-dimethylxanthene
2G Pd Xphos Chloro(2-dicyclohexylphosphino-2',4',6'-triisopropyl-l,r- biphenyl)[2-(2'-amino-1,1 '-biphenyl)]palladium(II)
t-butyl Xphos 2-di-tert-butylphosphino-2',4',6'-triisopropylbiphenyl
Representative Examples
Example 1: 2-({[4-({3'-[(4-{[(2-hydroxyethyl)amino]methyl}-2-methylphenoxy) methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}methoxy)-3-methylphenyl]methyl}amino)ethan- l-ol (1-1).
Figure AU2017305399A1_D0032
1-1
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The compound of Example 1 was synthesized via the route shown in the scheme below.
Figure AU2017305399A1_D0033
11-1
Figure AU2017305399A1_D0034
Step-2
Figure AU2017305399A1_D0035
II-2
Figure AU2017305399A1_D0036
IV-1
Pd(dppf)CI2, CsF dioxane. 110 °C Step-3
Figure AU2017305399A1_D0037
Figure AU2017305399A1_D0038
Step-7
1-1
Step-1'. methyl 3-bromo-2-methylbenzoate (II-2)
To a solution of 3-bromo-2-methylbenzoic acid II-l (10 g, 47 mmol) in 150 mL of MeOH was added concentrated H2SO4(10 mL) dropwise. The mixture was stirred at 70°C overnight. The solvent was evaporated and the remaining mixture was adjusted to pH 9 by adding saturated NaHCO3 solution. The mixture was extracted with ethyl acetate. The organic layers were dried over Na2SO4, filtered and concentrated. The residue was purified by column chromatography (petroleum ether : ethyl acetate = 150 : 1) to give the product as a yellow oil (10.1 g, 94%). 'H NMR (400 MHz, CDC13) δ 7.72 (d, J= 8.0 Hz, IH), 7.69 (d, J= 8.0 Hz, IH), 7.09 (t, J= 8.0 Hz, 7.6Hz, IH), 3.90 (s, 3H), 2.63 (s, 3H).
Step-2·. 2-methyl-3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl) benzoate (ΠΙ-1)
A mixture of methyl 3-bromo-2-methylbenzoate II-2 (3g, 13.1 mmol),
4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l,3,2-dioxaborolane (4 g, 15.7 mmol), Pd(dppf)Cl2 (670 mg, 0.9 mmol), KOAc (2.6 g, 26.2 mmol) in 150 mL of dioxane was stirred at 110°C under N2 overnight. The reaction mixture was filtered and concentrated, then extracted with ethyl acetate. The organic layer was dried over Na2SO4, filtered and concentrated. The crude was purified by column chromatography (petroleum ether : ethyl acetate = 80 : 1) to give a product as a green oil (2.9 g, 81%). 'H NMR (400 MHz, CDC13) δ 7.86-7.82 (m, 2H), 7.227.21 (m, IH), 3.88 (s, 3H), 2.74 (s, 3H), 1.36 (s, 12H).
Step-3'. dimethyl 2,2'-dimethyl-[l,r-biphenyl]-3,3'-dicarboxylate (IV-1)
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To a solution of 2-methyl-3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl) benzoate III-l (1.5 g, 5.4 mmol), methyl-3-bromo-2-methylbenzoate II-2 (1.1 g, 4.9 mmol), CsF (2.2 g, 14.7 mmol), and Pd(dppf)C12 (250 mg, 0.07 mmol) in 40 mL of dioxane was added 10 mL of H2O. The mixture was stirred at 110°C under N2 overnight. The reaction mixture was fdtered and concentrated, then extracted with ethyl acetate. The organic layer was dried over Na2SO4, fdtered and concentrated. The product was purified by column chromatography (petroleum ether : ethyl acetate = 80 : 1) to give a product as a green oil (1.5g, 92%). 'H NMR (400 MHz, CDC13) δ 7.86 (d, J= 1.6 Hz, 1H), 7.84 (d, J= 1.6 Hz, 1H), 7.29 (t, J= 7.6 Hz, 7.6 Hz, 2H), 7.24 (d, J= 1.2 Hz, 1H), 7.22 (d, J= 1.2 Hz, 1H), 3.92 (s, 6H), 2.22 (s, 6H).
Step-4·. (2,2'-dimethyl-[l,l'-biphenyl]-3,3'-diyl)dimethanol (IV-2)
To a solution of ester IV-1 (1.26 g, 4.2 mmol) in 50 ml of THF was added L1AIH4 (638 mg, 16.8 mmol) was added. The mixture was stirred at 0°C for five hours, then quenched with 0.6 mL H2O, 0.6 mL 10% NaOH and 1.8 mL H2O. The resulting mixture was filtered and extracted with ethyl acetate. The organic layer was dried over Na2SO4, filtered and concentrated. The product was purified by column chromatography (petroleum ether : ethyl acetate = 10 : 1) to give the product as a white solid (831 mg, 82%). 'H NMR (400 MHz, CDCh) δ 7.39 (d, J= 7.6 Hz, 2H), 7.23 (d, J= 7.6 Hz, 2H), 7.07 (d, J = 7.2 Hz, 2H), 4.77 (s, 4H), 2.03 (s, 6H).
Step-5'. 3,3'-bis(bromomethyl)-2,2'-dimethyl-l,l'-biphenyl, IV-3
To a solution of (2,2'-dimethyl-[l,l'-biphenyl]-3,3'-diyl)dimethanol IV-2 (300 mg, 1.2 mmol) in 50 mL of dichloromethane was added PBr3 (650 mg, 2.4 mmol) dropwise at 0°C. The mixture was stirred at 0°C overnight. It was quenched with water. The mixture was extracted with dichloromethane. The organic layer was dried over Na2SO4, filtered and concentrated. The product was purified by column chromatography (petroleum ether : ethyl acetate = 150: 1) to give the desired product IV-3 as a yellow solid (337 mg, 76%). 'H NMR (400 MHz, CDCI3) δ 7.35 (d, J= 7.6 Hz, 2H), 7.22 (t, J= 7.6 Hz, 7.2 HZ, 2H), 7.09 (d, J =
7.2 Hz, 2H), 4.59 (s, 4H), 2.09 (s, 6H).
Step-6'. 4,4'-(((2,2'-dimethyl-[l,l'-biphenyl]-3,3'-diyl)bis(methylene))bis(oxy))bis(3methyl benzaldehyde), IV-4
A mixture of 3,3'-bis(bromomethyl)-2,2'-dimethyl-l,l'-biphenyl, IV-3 (337 mg, 0.92 mmol), 4-hydroxy-3-methylbenzaldehyde (272 mg, 2.0 mmol) and K2CC>3 (386 mg, 2.8 mmol) in 20 mL of acetonitrile was stirred at room temperature overnight. The mixture was concentrated and extracted with ethyl acetate. The organic layer was dried over Na2SO4, filtered and concentrated. The product IV-4 was obtained a yellow solid (415 mg, 95%). 'H
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NMR (400 MHz, CDC13) δ 9.88 (s, 2H), 7.74-7.73 (m, 4H), 7.48 (d, J= 7.2 Hz, 2H), 7.30 (t, J = 7.6 Hz, 7.6 Hz, 2H), 7.18 (d, J= 7.6 Hz, 2H), 7.07 (d, J= 8.0 Hz, 2H), 5.21 (s, 4H), 2.33 (s, 6H), 2.07 (s, 6H).
Step-7: 2-({[4-({3[(4-{[(2-hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]2,2'-dimethyl-[l,l'-biphenyl]-3-yl}methoxy)-3-methylphenyl]methyl}amino)ethan-l-ol, 1-1.
A mixture of aldehyde IV-4 (100 mg, 0.21 mmol) and 2-aminoethanol (77 mg, 1.26 mmol) in 15 mL of EtOH was stirred at 85°C for 3 hours. When it cooled down to room temperature, NaBH4(32 mg, 0.84 mmol) was added at 0°C and stirred for two hours. It was quenched with 2 ml of saturated NaCl. The resulting mixture was concentrated, then diluted with dichloromethane, dried over Na2SO4, filtered and concentrated. The crude product was purified by column chromatography (dichloromethane : methanol = 10 : 1 ) to give the title compound 1-1 as a white solid (30 mg, 27%). 'H NMR (400 MHz, DMSO-de) δ 7.49 (d, J =
7.2 Hz, 2H), 7.29 (t, J= 7.6 Hz, 7.6 Hz, 2H), 7.10 (d, J= 9.6 Hz, 6H), 7.02 (d, J= 8.0 Hz, 2H), 5.13 (s, 4H), 4.45 (t, J = 52 Hz, 4.8 Hz, 2H), 3.60 (s, 4H), 3.45 (dd, J= 5.6 Hz, 5.2 Hz, 4H), 2.54 (d, J= 6.0 Hz, 4H), 2.17 (s, 6H), 2.01 (s, 6H). LCMS: m/z +569.52 [M+H]+.
Example 2: 2-{[(4-{[3-({4-[(azetidin-l-yl)methyl]-2-methylphenoxy}methyl)-2chlorophenyl]methoxy}-3-methylphenyl)methyl]amino}ethan-l-ol (1-2).
1-2
The compound of Example 2 was synthesized via the route shown in the scheme below.
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Figure AU2017305399A1_D0039
Figure AU2017305399A1_D0040
Step-1'. synthesis of l,3-bis(bromomethyl)-2-chlorobenzene
To the solution of 2-chloro-l,3-dimethylbenzene (10 g, 71 mmol) in CCU (200 mL) was added NBS (28 g, 157 mmol) and BOP (172 mg, 0.71 mmol). The mixture was heated to reflux for 16 hours. LC-MS indicated that the reaction was completed. The reaction mixture was cooled down, fdtered, and concentrated. The residue was crystallized with hexane to give white solid (5 g, 24%).
Step-2·. synthesis of 4,4'-(2-chloro-l,3-phenylene)bis(methylene)bis(oxy)bis(3-methyl benzaldehyde) (IV-5)
To the solution of l,3-bis(bromomethyl)-2-chlorobenzene (200 mg, 0.67 mmol) and
4-hydroxy-3-methylbenzaldehyde (192 mg, 1.4 mmol) in acetonitrile (10 mL) was added K2CO3 (195 mg, 138 mmol). The suspended solution was stirred at room temperature for 16 hours. The mixture was diluted with ethyl acetate (50 mL), and the organic layer was washed with brine. Then the organic layer was concentrated and purified by silica gel column (Petroleum ether : ethyl acetate = 2 : 1) to give the title compound as white solid (200 mg, 73%).
Step-3·. synthesis of 4-(3 -((4-(azeti din- l-ylmethyl)-2-methylphenoxy)methyl)-2chlorobenzyloxy)-3-methylbenzaldehyde (IV-6) and l-[(4-{[3-({4-[(azetidin-l-yl)methyl]-2 methylphenoxy}methyl)-2-chlorophenyl]methoxy}-3-methylphenyl)methyl]azetidine (1-3)
Figure AU2017305399A1_D0041
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To the solution of 4,4'-(2-chloro-l,3-phenylene)bis(methylene)bis(oxy)bis(3methylbenzaldehyde) IV-5 (200 mg, 0.49 mmol) in MeOH (5 mL) was added azetidine hydrochloride (46 mg, 0.49 mmol). After stirring for 30 minutes, NaBHsCN (30 mg, 0.49 mmol) was added and the mixture was stirred for 16 hours at room temperature. The reaction was quenched with the water, then extracted with ethyl acetate. The combined organic layer was dried, concentrated and purified by silica gel column (dichloromethane : MeOH = 25 : 1 to 15 : 1) to give IV-6 (60 mg) and 1-3 (50 mg).
l-[(4-{[3-({4-[(azetidin-l-yl)methyl]-2-methylphenoxy}methyl)-2chlorophenyl]methoxy}-3-methylphenyl)methyl]azetidine (1-3): 'H NMR (400MHz, CDCf ): δ 7.57 (d, J= 7.6 Hz, 2H), 7.34 (t, J= 7.6 Hz, 1H), 7.12 (s, 2H), 7.07 (d, J= 8.0 Hz, 2H), 6.84 (d, J= 7.2 Hz, 2H), 5.19 (s, 4H), 3.53 (s, 4H), 3.26 (t, J= 7.2 Hz, 8H), 2.31 (s, 6H), 2.11 (m, 4H). LCMS: m/z + 491.3 [M+H]+.
Step-4'. synthesis of 2-{[(4-{[3-({4-[(azetidin-l-yl)methyl]-2methylphenoxy } methyl)-2-chlorophenyl] methoxy } -3 -methylphenyl)methyl] amino } ethan-1 ol (1-2)
To the solution of aldehyde IV-6 (60 mg, 0.13 mmol) in MeOH (2 mL) was added 2aminoethanol (16 mg, 0.27 mmol). After stirring for 30 minutes, NaCNBH3 (17 mg, 0.27 mmol) was added and the mixture was stirred for 16 hours at room temperature. Quenched the reaction with the water, and extracted with ethyl acetate. The combined organic layer was concentrated and purified by silica gel column (dichloromethane : MeOH = 10 : l)to give 1-2 (30 mg). 1 H NMR (400 MHz, CDC13): δ 7.57 (d, J= 7.6 Hz, 2H), 7.34 (t, J= 7.6 Hz, 1H), 7.14-7.05 (m, 4H), 6.84 (t, J = 7.6 Hz, 2H), 5.19 (s, 4H), 3.73 (s, 2H), 3.66 (t, J = 4.2 Hz, 2H), 3.51 (s, 2H), 3.24 (t, J= 4.2 Hz, 4H), 2.78 (m, 2H), 2.32 (s, 3H), 2.31 (s, 3H), 2.12-2.08 (m, 2H). LCMS: m/z + 495.2 [M+H]+.
Example 3: (2S)-l-{[4-({3'-[(4-{[(2S)-2-carboxypiperidin-l-yl]methyl}-3,5dimethoxyphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}methoxy)-2,6dimethoxyphenyl]methyl}piperidine-2-carboxylic acid (1-4).
Ό COOH
Figure AU2017305399A1_D0042
The compound of Example 3 was synthesized via the route shown in the scheme below.
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Figure AU2017305399A1_D0043
Figure AU2017305399A1_D0044
COOH
HN'iSb]
NaBH3CN, MeOH
Figure AU2017305399A1_D0045
4,4'-(2,2'-dimethylbiphenyl-3,3'-diyl)bis(methylene)bis(oxy)bis(2,6dimethoxybenzaldehyde) (IV-7)
To a solution of 3,3'-bis(bromomethyl)-2,2'-dimethyl-l,r-biphenyl, IV-3 (500 mg,
1.4 mmol), 4-hydroxy-2,6-dimethoxybenzaldehyde (497 mg, 2.7 mmol), K2CO3 (447 mg, 3.2 mmol) in 10 mL of DMF was stirred at room temperature overnight. The mixture was diluted with water, filtered to give a pale solid (600 mg, 77%). The crude was used for next step without further purification. XH NMR (400 MHz, CDC13) δ 10.37 (s, 2H), 7.34-7.17 (m, 6H), 6.20 (s, 4H), 5.18 (s, 4H), 3.89 (s, 12H), 2.10 (s, 6H).
Step-2'. (S)-l-(4-((3'-((4-formyl-3,5-dimethoxyphenoxy)methyl)-2,2'dimethylbiphenyl-3-yl)methoxy)-2,6-dimethoxybenzyl)piperidine-2-carboxylic acid (IV-8) & (2S)-l-{[4-({3'-[(4-{[(2S)-2-carboxypiperidin-l-yl]methyl)-3,5-dimethoxyphenoxy)methyl]2,2'-dimethyl-[l,r-biphenyl]-3-yl}methoxy)-2,6-dimethoxyphenyl]methyl}piperidine-2carboxylic acid (1-4)
To a solution of aldehyde IV-7 (300 mg, 0.52 mmol) in MeOH (5 mL) was added (S)piperidine-2-carboxylic acid (68 mg, 0.52 mmol) and Cat. AcOH. After stirring for 30 minutes, NaBH3CN (32 mg, 0.52 mmol) was added and the mixture was stirred for 16 hours at room temperature. Quenched the reaction with the water, and extracted with ethyl acetate.
The combined organic layer was concentrated and purified by silica gel column (dichloromethane : MeOH = 25 : 1 to 10 : 1) to give IV-8 (110 mg) and 1-4 (30 mg). 1-4: 'H NMR (400MHz, DMSO-J6): 67.50 (d, J= 7.5 Hz, 2H), 7.30 (t, J= 7.5 Hz, 2H), 7.11 (d, J =
7.5 Hz, 2H), 6.42 (s, 4H), 5.20 (s, 4H), 4.10 (s, 4H), 3.79 (s, 13H), 3.12 (s, 2H), 3.04 (s, 2H),
2.63 (s, 2H), 2.02 (s, 6H), 1.81 (s, 4H), 1.55 (s, 4H), 1.39 (s, 4H). LCMS: m/z + 819.43 [M+Na]+.
Example 4: (2S)-l-{[4-({3'-[(4-{ [(2-hy droxy ethyl)amino]methyl )-3,5dimethoxyphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}methoxy)-2,6dimethoxyphenyl]methyl}piperidine-2-carboxylic acid (1-5).
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Figure AU2017305399A1_D0046
Figure AU2017305399A1_D0047
mg, 0.16 mmol) in MeOH (5 mL) was added 2aminoethanol (20 mg, 0.32 mmol) and catalytical amount of acetic acid. After stirring for 30 minutes, NaBH3CN (20 mg, 0.32 mmol) was added and the mixture was stirred for 16 hours at room temperature. The reaction was quenched with the water, and extracted with AcOEt. The combined organic layers were dried over anhydrous Na2SO4 and concentrated. The residue was purified by silica gel column (dichloromethane : MeOH = 25 : 1 to 10 : 1) to give 1-5 (40 mg). 'H NMR (400MHz, DMSO-Jri): 7.49 (d, J = 7.2 Hz, 2H), 7.29 (t, J = 7.2 Hz, 2H), 7.10 (s, 2H), 6.41 (s, 2H), 6.35 (s, 2H), 5.19 (s, 2H), 5.16 (s, 2H), 4.10 (s, 2H), 3.78 (s, 6H), 3.75 (s, 6H), 3.61 (s, 2H), 3.22-3.03 (m, 6H), 2.61 (s, 1H), 2.02 (s, 6H), 1.82 (s, 2H), 1.55 (s, 2H), 1.39 (s, 2H). LCMS: m/z + 729.45 [M+H]+.
Example 5: 2-({[4-({3'-[(4-{[(2-hydroxyethyl)amino]methyl}-3,5-dimethoxy phenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}methoxy)-2,6-dimethoxyphenyl] methyl }amino)ethan-l-ol (1-6).
Figure AU2017305399A1_D0048
h2n
NaBH3CN, MeOH
Figure AU2017305399A1_D0049
To a solution of aldehyde IV-7 (80 mg, 0.14 mmol) in MeOH (5 mL) was added 2aminoethanol (34 mg, 0.56 mmol) and catalyst AcOH. After stirring for 30 minutes, NaBH3CN(34 mg, 0.56 mmol) was added and the mixture was stirred for 16 hours at room temperature. The combined organic layers were dried over anhydrous Na2SO4 and concentrated. The residue was purified by silica gel column (dichloromethane:MeOH = 25 : 1 to 10 : 1) to give 1-6 (40 mg): 'H NMR (400 MHz, CDC13) δ 7.45 (d, J= 7.4 Hz, 2H), 7.29 (t, J= 7.6 Hz, 2H), 7.15 (d, J= 7.5 Hz, 2H), 6.24 (s, 4H), 5.09 (s, 4H), 4.43 ( brs, 2OH), 3.81 (s, 12H), 3.64-3.63 (m, 4H), 3.49-3.22 (m, 8H), 2.08 (s, 6H). LCMS: m/z + 683.35 [M+Na]+.
Example 6: l-{[4-({3'-[(4-{[(2-hydroxyethyl)amino]methyl}-2-methyl phenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}methoxy)-2methoxyphenyl]methyl}azetidin-3-ol (1-7).
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Figure AU2017305399A1_D0050
1-7
The preparation of Intermediate (Π-5): 1 -(4-((3-bromo-2-methylbenzyl)oxy)-2methoxybenzyl)azetidin-3-ol
Figure AU2017305399A1_D0051
Step-1 Step-2
Step-1'. 4-((3-bromo-2-methylbenzyl)oxy)-2-methoxybenzaldehyde (II-4)
Figure AU2017305399A1_D0052
Diisopropyl azodicarboxylate (1.1 mmol) in tetrahydrofuran (1.5 mL) is added dropwise to a cooled (0 °C) solution of 4-hydroxy-2-methoxybenzaldehyde (1.1 mmol), triphenyl phosphine (1.1 mmol) and (3-bromo-2-methylphenyl)methanol II-3 (1.0 mmol) in dry tetrahydrofuran (3 mL). The resulting reaction mixture is allowed to slowly warm to room temperature with stirring overnight. The product is filtered from the reaction mixture using a Buchner filter funnel and rinsed with tetrahydro furan (approx. 3 mL) then dried in vacuo at room temperature to yield the title compound II-4. The crude is used for next step without further purification.
Step-2'. 1-(4-((3-bromo-2-methylbenzyl)oxy)-2-methoxybenzyl)azetidin-3-ol(II-5)
Figure AU2017305399A1_D0053
A mixture of 4-((3-bromo-2-methylbenzyl)oxy)-2-methoxy benzaldehyde (1 mmol), azetidin-3-ol (3 mmol) and AcOH (5 mmol) in DMF is stirred at RT for 4-16 h. Then sodium cyanoborohydride (3 mmol) is added and the mixture is stirred at RT until reductive amination is complete (~16 h). Further purification of the crude via reverse
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II-5
The preparation of Intermediate (ΠΙ-3): 3-methyl-4-((2-methyl-3-(4,4,5,5tetramethyl-l,3,2-dioxaborolan-2-yl)benzyl)oxy)benzaldehyde o
H
A solution of 4-hydroxy-3-methylbenzaldehyde (1.1 mmol), triphenyl phosphine (1.1 mmol) and (2-methyl-3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyl)methanol (1.0 mmol) in dry THF (6 mL) is cooled in an ice bath. Diisopropyl azodi carb oxy late (1.1 mmol) in THF (6 mL) is added dropwise. The resulting yellow solution is allowed to slowly warm to room temperature while stirring overnight. The solvent is removed and the residue is purified on a silica column with a mixture of hexanes:ethyl acetate. Collected fractions to afford the desired product (ΠΙ-3).
The preparation of l-{[4-({3'-[(4-{[(2-hydroxyethyl)amino]methyl} phenoxy)methyl]-2,2'-dimethyl-[l,T-biphenyl]-3-yl}methoxy)-2-methoxyphenylmethyl} azetidin-3-ol (1-7)
2nd G Pd XPhos
OH
III-3
11-5
Step-1
ΌΗ
I-7
Step-1'. 4-((3'-((4-((3-hydroxyazetidin-l-yl)methyl)-3-methoxy phenoxy)methyl)-2,2'dimethyl-[ 1,1 '-biphenyl]-3 -yl)methoxy)benzaldehyde
ΌΗ
Tetrahydrofuran solvent and aqueous 0.5M potassium tribasic phosphate solutions were sparged with nitrogen for 15 minutes prior to dispensing for use. In a round-bottom flask charge 4-((3'-((4-((3-hydroxyazetidin-l-yl)methyl)-3-methoxy
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III-3 (1.2 mmol), 1-(4-((3-bromo-2-methylbenzyl)oxy)-2-methoxybenzyl)azetidin-3-ol (II-5) (1.0 mmol) and 2G Pd Xphos precatalyst (0.03 mmol)(CAS number 1310584-145), add previously deoxygenated tetrahydrofuran (5 mL) and 0.5 M aq potassium phosphate, tribasic solution (5 mL, 2.5 mmol), place under nitrogen and sparged with additional nitrogen for 10 minutes. The reaction is stirred under nitrogen at room temperature for 2 days. Ethyl acetate (12 mL) is added to the reaction followed by 8 mL of brine then the reaction is partitioned in a separatory funnel. The organic extract is washed ( lx) with brine and dried over magnesium sulfate, filtered and solvent removed in vacuo using a rotary evaporator. The crude reaction product is purified by silica gel chromatography eluting with an appropriate gradient of ethyl acetate/hexanes to yield a pure title compound.
Step-2·: l-{[4-({3'-[(4-{[(2-hydroxyethyl)amino]methyl}phenoxy)methyl]-2,2'dimethyl-[l,l'-biphenyl]-3-yl}methoxy)-2-methoxyphenyl]methyl}azetidin-3-ol (1-7)
A mixture of 4-((3 '-((4-((3-hydroxyazetidin-l-yl)methyl)-3 -methoxyphenoxy) methyl)-2,2'-dimethyl-[l,l'-biphenyl]-3-yl)methoxy)benzaldehyde (1 mmol), 2-aminoethan-
1- ol (3 mmol) and AcOH (5 mmol) in DMF is stirred at RT for 4-16 h. Then Sodium triacetoxyborohydride (3 mmol) is added and the mixture is stirred at RT until reductive amination is complete (typically overnight). Further purification of the crude via reverse phase preparative HPLC yields the desired product 1-7.
Example 7: 2- {[(6- {[3 -(5 - {[(5 - {[(2-hydroxyethyl)amino]methyl}-6-methoxypyridin-
2- yl)oxy]methyl}-4-methylthiophen-3-yl)-2-methylphenyl]methoxy}-2-methoxypyridin-3yl)methyl]amino}ethan-l-ol (1-8).
Figure AU2017305399A1_D0054
Figure AU2017305399A1_D0055
The Preparation of Intermediate (Π-6): (4-bromo-3-methylthiophen-2-yl)methanol bh3-thf Dr 1 * 'XSx^OH THF, -5°C; 3h ^-S
-5°C to rt
11-6
To a solution of 4-bromo-3-methylthiophenecarboxylic acid (2 g; 0.95 mmol) in THF (20 mL) was added borane-THF complex (IM, 18.19 mL; 18.19 mmol) dropwise, at 0°C. Subsequently, the reaction mixture was stirred for 1 hour at 60°C. To this
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100%) was used without further purification. (Ref: WO 2012004378).
Figure AU2017305399A1_D0056
111-2
Figure AU2017305399A1_D0057
11-6
Figure AU2017305399A1_D0058
h2n
Figure AU2017305399A1_D0059
AcOH, NaBH3CN eqv.
Step-3
Step -2
Figure AU2017305399A1_D0060
Step-1'. (4-(3-(hydroxymethyl)-2-methylphenyl)-3-methylthiophen-2-yl)methanol
Figure AU2017305399A1_D0061
Tetrahydrofuran solvent and aqueous 0.5M potassium tribasic phosphate solutions were sparged with nitrogen for 15 minutes prior to dispensing for use. In a round-bottom flask charge (2-methyl-3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2yl)phenyl)methanol III-2 (1.2 mmol), (4-bromo-3-methylthiophen-2-yl)methanol (II-6) (1.0 mmol) and 2G palladium Xphos precatalyst (0.03 mmol)(CAS number 131058414-5), add previously deoxygenated tetrahydrofuran (5 mL) and 0.5 M aq potassium phosphate, tribasic solution (5 mL, 2.5 mmol), place under nitrogen and sparged with additional nitrogen for 10 minutes. The reaction is stirred under nitrogen at room temperature for 2 days. Ethyl acetate (12 mL) is added to the reaction followed by 8 mL of brine then the reaction is partitioned in a separatory funnel. The organic extract is washed (lx) with brine and dried over magnesium sulfate, filtered and solvent removed in vacuo using a rotary evaporator. The crude reaction product is purified by silica gel chromatography eluting with an appropriate gradient of ethyl acetate/hexanes to yield a pure title compound.
Step-2'. 6-{[3-(5-{[(5-formyl-6-methoxypyridin-2-yl)oxy]methyl}-4methylthiophen-3-yl)-2-methylphenyl]methoxy}-2-methoxypyridine-3-carbaldehyde
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Figure AU2017305399A1_D0062
O Cty
Cesium carbonate (4.0 mmol, 2 eqv.), palladium(II) acetate (0.2 mmol, 0.1 eqv.),
2-di-tert-butylphosphino-2',4',6'-triisopropylbiphenyl (t-butyl Xphos) (0.4 mmol, 0.2 eqv.), 6-chloro-2-methoxynicotinaldehyde (2.6 mmol, 1.3 eqv.), and (4-(3-(hydroxymethyl)-2methylphenyl)-3-methylthiophen-2-yl)methanol (1.0 mmol, 2N) are combined in a 25 mL round bottom flask equipped with a stir bar. Toluene (12 mL) is added and the mixture is purged with a stream of argon for 5 minutes. The reaction is sealed and heated at 80 °C overnight. The soluble portion of the crude reaction is charged to a 25g silica gel column with dichloromethane and chromatographed with an appropriate gradient of ethyl acetate in hexanes. A fraction containing a compound tested positive for an aldehyde using 2,4 dinitrophenyl hydrazine stain. This aldehyde-positive fraction is isolated and used for next step reaction.
Step-3: 2-{ [(6-{[3-(5-( [(5-{[(2-hydroxyethyl)amino]methyl}-6-methoxypyridin-
2-yl)oxy ] methyl} -4-methylthiophen-3 -yl)-2-methylphenyl]methoxy } -2- methoxypyridin-3-yl)methyl]amino}ethan-l-ol (1-8)
A mixture of 6-( [3-(5-( [(5-formyl-6-methoxypyridin-2-yl)oxy]methyl}-4-methyl thiophen-3-yl)-2-methylphenyl]methoxy}-2-methoxypyridine-3-carbaldehyde (1 mmol, 2N),
2-aminoethan-l-ol (6 mmol, 3 eqv.) and AcOH (10 mmol, 5 eqv.) in DMF (5 mL) is stirred at RT for 4-16 h. Then sodium cyanoborohydride (6 mmol, 3 eqv.) is added and the mixture is stirred at RT until reductive amination is complete (typically overnight). Further purification of the crude via reverse phase preparative HPLC yields the desired product 1-8.
Example 8: 2-(( [4-((4-((4-([(2-hydroxyethyl)amino]methyl}-3-methoxy phenoxy) methyl]-3-methylthiophen-2-yl}methoxy)-2-methoxyphenyl]methyl} amino)ethan-l-ol (1-9)
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Figure AU2017305399A1_D0063
h2n~oh eqv.
Figure AU2017305399A1_D0064
Step-1
Figure AU2017305399A1_D0065
Step-2
AcOH, NaBHjCN
Step-3
Figure AU2017305399A1_D0066
I-9
Step-T. (3-methylthiophene-2,4-diyl)dimethanol
Figure AU2017305399A1_D0067
To a round-bottom flask is charged dimethyl 3-methylthiophene-2,4 dicarboxylate (1.0 mmol, 2N) and tetrahydrofuran (10 mL). The mixture is stirred until a clear solution is obtained. The solution is cooled to - 40°C and lithium borohydride (4 mmol, 2 eqv.) is added portion wise over 15 minutes. After all the lithium borohydride is added, the reaction is slowly brought to room temperature and stirred overnight. When TLC or LCMS analysis showed that the starting material is consumed, saturated aqueous ammonium chloride is charged to a multineck round-bottom flask and cooled to - 5 °C (inner temperature). Added crude reaction slowly over 15 minutes. After addition is complete the temperature is maintained at - 5 °C for 20 minutes. The reaction is diluted with dichloromethane (15 ml) and the layers are separated. The aqueous layer is extracted with dichloromethane (1 x 15 ml) and the combined organic portions are washed with 1.5 N aqueous hydrochloric acid (1 x 1.3 ml), saturated aqueous sodium chloride (1 x 1.3 ml) and dried over sodium sulfate. The solvent was removed under reduced pressure to give a crude product. The crude material was dissolved in minimum amount of di chloromethane and cooled in ice bath. Petroleum ether was added until white solid formed. The solid was collected by filtration, washed with petroleum ether and dried under vacuum to give the title compound. (Ref WO 2010125102 Al )
Step-2'. 4-({4-[(4-formyl-3-methoxyphenoxy)methyl]-3-methylthiophen-2yl}methoxy)-2-methoxybenzaldehyde
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O O
Figure AU2017305399A1_D0068
Diisopropyl azodicarboxylate (2.2 mmol, 1.1 eqv.) in tetrahydrofuran (2.5 mL) is added dropwise to a cooled (0 °C) solution of 4-hydroxy-2-methoxybenzaldehyde (2.2 mmol, 1.1 eqv.), triphenyl phosphine (2.2 mmol, 1.1 eqv.) and (3-methylthiophene-2,4diyl)dimethanol (1.0 mmol, 2N) in dry tetrahydrofuran (6 mL). The resulting reaction mixture is allowed to slowly warm to room temperature with stirring overnight. The product is filtered from the reaction using a Buchner filter funnel and rinsed with tetrahydrofuran (approx. 5 mL) then dried in vacuo at room temperature to yield the title compound. The crude is used for next step without further purification.
Step-3·. 2-({[4-({4-[(4-{[(2-hydroxyethyl)amino]methyl}-3-methoxyphenoxy) methyl]-3-methylthiophen-2-yl}methoxy)-2-methoxyphenyl]methyl}amino)ethan-l-ol (1-9)
Figure AU2017305399A1_D0069
A mixture 4-({4-[(4-formyl-3-methoxyphenoxy)methyl]-3-methylthiophen-2yl}methoxy)-2-methoxybenzaldehyde (1 mmol, 2N), 2-aminoethan-l-ol (6 mmol, 3 eqv.) and AcOH (10 mmol, 5 eqv.) in DMF (5 mL) is stirred at RT for 4-16 h. Then sodium cyanoborohydride (6 mmol, 3 eqv.) is added and the mixture is stirred at RT until reductive amination is complete (typically overnight). The crude is purified via reverse phase preparative HPLC to give the title compound 1-9.
Example 9: 2-({4-[(azetidin-l-yl)methyl]-2-methylphenoxy}methyl)-6-{[4-({[(4oxoazetidin-2-yl)methyl]amino}methyl)phenoxy]methyl}benzonitrile (1-10)
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Figure AU2017305399A1_D0070
Step-4
111-5
Figure AU2017305399A1_D0071
Step-3
1-10
Step-1: methyl 2-cyano-3-((4-formyl-2-methylphenoxy)methyl)benzoate
Figure AU2017305399A1_D0072
A solution of 4-hydroxy-3-methylbenzaldehyde (1.1 mmol), triphenyl phosphine (1.1 mmol) and methyl 2-cyano-3-(hydroxymethyl)benzoate (1.0 mmol) in dry THF (5 mL) is cooled in an ice bath. Diisopropyl azodi carb oxy late (1.1 mmol) in THF (5 mL) is added dropwise. The resulting yellow solution is allowed to slowly warm to room temperature while stirring overnight. The solvent is removed and the residue is purified on a silica column with a mixture of hexanes:ethyl acetate. Collected fractions to afford the desired product.
Step-2: methyl 3-((4-(azetidin-1 -ylmethyl)-2-methylphenoxy)methyl)-2cy anobenzoate
N
Figure AU2017305399A1_D0073
A solution of tetramethyl ammonium triacetoxyborohydride (2.0 mmol, 2 eqv.) and azetidine hydrochloride salt (2.0 mmol, 2 eqv.) in dichloromethane (12 mL) is added to a solution of methyl 2-cyano-3-((4-formyl-2-methylphenoxy)methyl)benzoate (1.0 mmol, 1 eqv.) in dichloromethane (12 mL). The reaction mixture is stirred at room temperature overnight. The reaction is quenched by the addition of saturated sodium
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Step-3. 2-((4-(azetidin-l-ylmethyl)-2-methylphenoxy)methyl)-6-
Figure AU2017305399A1_D0074
Figure AU2017305399A1_D0075
To a round-bottom flask is charged methyl 3-((4-(azetidin-l-ylmethyl)-2methylphenoxy)methyl)-2-cyanobenzoate (1.0 mmol) and tetrahydrofuran (5 mL). The mixture is stirred until a clear solution is obtained. The solution is cooled to - 40°C and lithium borohydride (2 mmol, 2 eqv.) is added portion wise over 15 minutes. After all the lithium borohydride is added, the reaction is slowly brought to room temperature and stirred overnight. When TLC or LCMS analysis showed that all starting material is consumed, saturated aqueous ammonium chloride is charged to a multineck roundbottom flask and cooled to - 5 °C (inner temperature) to which is added the crude reaction mixture slowly over 15 minutes. After addition is complete the temperature is maintained at - 5 °C for 20 minutes. The reaction is diluted with di chloromethane (15 ml) and the layers are separated. The aqueous layer is extracted with dichloromethane (1 x 15 ml) and the combined organic portions are washed with 1.5 N aqueous hydrochloric acid (1 x 1.3 ml ), saturated aqueous sodium chloride ( 1 x 1.3 ml ) and dried over sodium sulfate. The solvent was removed under reduced pressure to give a crude product. The crude material was dissolved in minimum amount of di chloromethane and cooled in ice bath. Petroleum ether was added until white solid formed. The solid was collected by filtration, washed with petroleum ether and dried under vacuum to give the title compound. (WO 2010125102 Al )
Step-4. 2-((4-(azetidin-l-ylmethyl)-2-methylphenoxy)methyl)-6-((4formylphenoxy) methyl)benzonitrile (ΠΙ-5)
Figure AU2017305399A1_D0076
A solution of 4-hydroxybenzaldehyde (1.1 mmol), triphenyl phosphine (1.1 mmol) and 2-((4-(azetidin-1 -ylmethyl)-2-methylphenoxy)methyl)-6-(hydroxymethyl)benzonitrile
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Step-5·: 2-({4-[(azetidin-l-yl)methyl]-2-methylphenoxy}methyl)-6-{[4-({[(4oxoazetidin-2-yl)methyl ] amino }methyl)phenoxy (methyl (benzonitrile (1-10)
N
Figure AU2017305399A1_D0077
A mixture 2-((4-(azetidin-1 -ylmethyl)-2-methylphenoxy)methyl)-6-((4formylphenoxy) methyl) benzonitrile (1 mmol), 4-(aminomethyl)azetidin-2-one (3 mmol, 3 eqv.) and AcOH (5 mmol, 5 eqv.) in DMF (5 mL) is stirred at RT for 4-16 h. Then sodium cyanoborohydride (3 mmol, 3 eqv.) is added and the mixture is stirred at RT until reductive amination is complete (typically overnight). The crude is purified via reverse phase preparative HPLC to give the title compound 1-10.
Examples 10 - 12
Figure AU2017305399A1_D0078
111-5 1-11,1-12 & 1-13
General procedure of reductive amination:
A mixture aldehyde (1 equiv), appropriate amine or amino acid (3 equiv) and AcOH (5 equiv) in DMF was stirred at RT for 4-16 h. Then sodium cyanoborohydride (3 equiv) was added and the mixture was stirred at RT until reductive amination is complete (typically overnight). Product was purified via preparative HPLC with the following conditions: Column: XBridge Cl8, 19 x 200 mm, 5-pm particles; Mobile Phase A: 5:95 acetonitrile: water with 10-mM ammonium acetate; Mobile Phase B: 95:5 acetonitrile: water with 10-mM ammonium acetate; Gradient: 20-60% B over 30 minutes, then a 5- minute hold at 100% B; Flow: 20 mL/min. Fractions containing the desired product were combined and dried via centrifugal evaporation.
Example 10: N-(2-{[(4-{[3-({4-[(azetidin-l-yl)methyl]-2-methyl phenoxy}methyl)-2-cyanophenyl]methoxy}phenyl)methyl]amino}ethyl)acetamide (1-11)
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Figure AU2017305399A1_D0079
Compound 1-11 is prepared in the same manner as the general procedure described above using aldehyde (ΠΙ-5) and N-(2-aminoethyl)acetamide.
Example 11: 2-({4-[(azetidin-l-yl)methyl]-2-methylphenoxy} methyl) -6-[(4{[(2-hydroxyethyl)amino]methyl}phenoxy)methyl]benzonitrile (1-12)
Figure AU2017305399A1_D0080
Compound 1-12 is prepared in the same manner as the general procedure described above using aldehyde (ΠΙ-5) and 2-aminoethan-l-ol.
Example 12: 2-({4-[(azetidin-l-yl)methyl]-2-methylphenoxy} methyl)-6-({4-[(azetidin1 -yl)methy 1] phenoxy } methyl)benzonitrile (1-13)
Figure AU2017305399A1_D0081
Compound 1-13 is prepared in the same manner as the general procedure described above using aldehyde (ΠΙ-5) and azetidine hydrochloride salt.
Example 13: 2-({[4-({4-[(4-{[(2-hydroxyethyl)amino]methyl}-3,5 dimethoxyphenoxy) methyl]-lH-indol-l-yl}methyl)phenyl]methyl} amino)ethan-l-ol (I14)
Figure AU2017305399A1_D0082
Figure AU2017305399A1_D0083
AcOH, NaBH3CN
Figure AU2017305399A1_D0084
eqv.
Step-3
Figure AU2017305399A1_D0085
Step-1'. 4-((lH-indol-4-yl)methoxy)-2,6-dimethoxybenzaldehyde
Figure AU2017305399A1_D0086
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A solution of 4-hydroxy-2,6-dimethoxybenzaldehyde (1.1 mmol), triphenyl phosphine (1.1 mmol) and (lH-indol-4-yl)methanol (1.0 mmol) in dry THF (5 mL) is cooled in an ice bath. Diisopropyl azodi carb oxy late (1.1 mmol) in THF (5 mL) is added dropwise. The resulting yellow solution is allowed to slowly warm to room temperature while stirring overnight. The solvent is removed and the residue is purified on a silica column with a mixture of hexanes:ethyl acetate. Collected fractions to afford the desired product.
Step-2'. 4-((l-(4-formylbenzyl)-lH-indol-4-yl)methoxy)-2,6-dimethoxy benzaldehyde
Figure AU2017305399A1_D0087
NaH (1 mol equiv) is dissolved in anhydrous DMF (5 mL/mmol) under argon and cooled to 0 °C. To this mixture is added 4-((lH-indol-4-yl)methoxy)-2,6-dimethoxy benzaldehyde (1 mol equiv) dissolved in anhydrous DMF (3 mL/mmol). The reaction is stirred for 15 min at 0 °C followed by the addition of 4-(bromomethyl) benzaldehyde (1 mol equiv) in anhydrous DMF (2 mL/mmol). The reaction is stirred for 2 h at 70 °C and then quenched by the addition of H2O (30 mL). The organic products are extracted with EtOAc (3 x 30 mL), washed with H2O (2 x 30 mL), brine (15 mL), dried over Na2SO4, filtered and concentrated in vacuo to yield the title compound.
Step-3:2-({[4-({4-[(4-{[(2-hydroxyethyl)amino]methyl}-3,5dimethoxyphenoxy)methy 1] -1 H-indol-1 -y 1 }methy l)pheny l]methy 1} amino)ethan-1 -ol (I14)
Figure AU2017305399A1_D0088
A mixture 4-((1 -(4-formylbenzyl)-lH-indol-4-yl)methoxy)-2,6-dimethoxy benzaldehyde (1 mmol), 2-aminoethan-1 -ol (3 mmol, 3 eqv.) and AcOH (5 mmol, 5 eqv.) in DMF (5 mL) is stirred at RT for 4-16 h. Then sodium cyanoborohydride (3 mmol) is added and the mixture is stirred at RT until reductive amination is complete (typically overnight). The crude is purified via reverse phase preparative HPLC to give the title compound 1-14.
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Example-14: 2-({[4-({l-[(4-{[(2-hydroxyethyl)amino]methyl} phenyl)methyl]lH-indazol-4-yl}methoxy)-2,6-dimethoxyphenyl]methyl} amino)ethan-l-ol (1-15)
Figure AU2017305399A1_D0089
Compound 1-15 is prepared in the same manner as the procedure described for I14 except using (lH-indazol-4-yl)methanol (III-6) and azetidine hydrochloride salt.
Example-15: 2,6-bis[(4-{[(2-hydroxyethyl)amino]methyl}-3methoxyphenoxy)methyl] benzonitrile, 1-16
Figure AU2017305399A1_D0090
o
Figure AU2017305399A1_D0091
Step-2
HO eqv.
nh2
AcOH, NaBH3CN
Step-3
Figure AU2017305399A1_D0092
1-16
The detailed procedures for step 1-3 are described in Example 9.
Example-16: preparation of compound 1-17,1-18,1-19,1-20 and 1-21.
Figure AU2017305399A1_D0093
Figure AU2017305399A1_D0094
Standard protocol for alkylation:
A mixture of 3,3'-bis(bromomethyl)-2,2'-dimethyl-l,l'-biphenyl, IV-3 (1.0 mmol), an appropriate hydroxy-aryl aldehyde(phenols) (2.17 mmol) and K2CO3 (2.5 mmol) in 20 mL of acetonitrile (or DMF) is stirred at room temperature or elevated temperature for 16 h. The mixture was concentrated and extracted with ethyl acetate. The organic layer was dried over Na2SO4, filtered and concentrated. The crude product is subjected purification by normal column chromatography or reversed phase HPLC.
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Standard protocol for reductive amination.
A mixture of aldehyde (1.0 mmol, 2N) and an appropriate amine (6.0 mmol, 3 eqv) in 60 mL of EtOH was stirred at 85°C for 3 hours. When it cooled down to room temperature, NaBHaCN (6 mmol, 3 eqv) was added at 0°C and stirred for two hours. It is quenched with 2 ml of saturated NaCl. The resulting mixture is concentrated, then diluted with di chloromethane, dried over Na2SO4, filtered and concentrated. The crude product is purified by column chromatography (dichloromethane : methanol ) to give the title compound 1-1721.
Example-17: preparation of compound 1-22,1-23,1-24 and 1-25.
Figure AU2017305399A1_D0095
Figure AU2017305399A1_D0096
NHRR', 2 eqv.
NaBH3CN, MeOH
Figure AU2017305399A1_D0097
1-22, 1-23, 1-24 & 1-25
Standard protocol for C-0 coupling reaction·.
Cesium carbonate (4.0 mmol, 2 eqv.), palladium(II) acetate (0.2 mmol, 0.1 eqv.), 2-di-tert-butylphosphino-2',4',6'-triisopropylbiphenyl (t-butyl Xphos) (0.4 mmol, 0.2 eqv.), 6-chloro-2-methoxynicotinaldehyde (2.6 mmol, 1.3 eqv.), and (2,2'-dimethyl-[l,rbiphenyl]-3,3'-diyl)dimethanol IV-2 (1.0 mmol, 2N) are combined in a 25 mL round bottom flask equipped with a stir bar. Toluene (12 mL) is added and the mixture is purged with a stream of argon for 5 minutes. The reaction is sealed and heated at 80 °C overnight. The soluble portion of the crude reaction is charged to a silica gel column with di chloromethane and chromatographed with an appropriate gradient of ethyl acetate in hexanes. A fraction containing a compound tested positive for an aldehyde using 2,4 dinitrophenyl hydrazine stain. This aldehyde-positive fraction is isolated and used for next step reaction.
Next step is followed by a standard protocol for reductive amination described for I- to 1-21.
Example-18: 2-({[4-({3'-[(4-{[(2-hydroxyethyl)amino]methyl}-3,5dimethoxyphenoxy) methyl]-2'-methyl-[l,r-biphenyl]-3-yl}methoxy)-2,643
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Figure AU2017305399A1_D0098
Figure AU2017305399A1_D0099
Figure AU2017305399A1_D0100
Step-1: dimethyl 2-methyl-[l,l'-biphenyl]-3,3'-dicarboxylate (IV-10)
To a solution of (3-(methoxycarbonyl) phenyljboronic acid III-4 (1.10 mmol), methyl-3-bromo-2-methylbenzoate II-2 (1.0 mmol), CsF (3.0 mmol), and Pd(dppf)C12(0.02 mmol) in 8 mL of dioxane is added 2 mL of H2O. The mixture is stirred at 110°C under N2 overnight. The reaction mixture is filtered and concentrated, then extracted with ethyl acetate. The organic layer is dried over Na2SO4, filtered and concentrated. The product is purified by column chromatography (petroleum ether : ethyl acetate) to yield title product IV-10.
Step-2: (2-methyl-[l,l'-biphenyl]-3,3'-diyl)dimethanol (IV-11)
To a solution of dimethyl 2-methyl-[l,l'-biphenyl]-3,3'-dicarboxylate, IV-10 (1.0 mmol) in 12 ml of THF was added LiAlH4 (4.0 mmol) is added. The mixture is stirred at 0°C for five hours, then quenched with 0.15 mL H2O, 0.15 mL 10% NaOH and 0.45 mL H2O. The resulting mixture is filtered and extracted with ethyl acetate. The organic layer is dried over Na2SO4, filtered and concentrated. The product was purified by column chromatography (petroleum ether/ethyl acetate) to yield the title molecule IV-11.
Step-3'. 3,3'-bis(bromomethyI)-2-methyl-l, 1 '-biphenyl, IV-12
To a solution of (2-methyl-[l,l'-biphenyl]-3,3'-diyl)dimethanol IV-11 (1.0 mmol) in 40 mL of dichloromethane is added PBr3 (2.0 mmol) dropwise at 0 °C. The mixture is stirred at 0°C overnight. It is quenched with water. The mixture was extracted with dichloromethane. The organic layer is dried over Na2SO4, filtered and concentrated. The product is purified by column chromatography (petroleum ether : ethyl acetate) to give the desired product IV-12.
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Step-4&5'. follow the standard alkylation and reductive amination procedures described for Example-16 to yield the title compound 1-26.
Example-19: 3,3'-bis({[(5-{[(2-hydroxyethyl)amino]methyl}-6methoxypyridin-2-yl)oxy]methyl})-[l,l'-biphenyl]-2-carbonitrile, 1-30.
Figure AU2017305399A1_D0101
Pd(dppf)CI2, KOAc dioxane, 110 °C
Step-1
Figure AU2017305399A1_D0102
IV-14
Step-2
IV-15
Figure AU2017305399A1_D0103
Step-3
Step-1: dimethyl 2-cyano-[l,T-biphenyl]-3,3!-dicarboxylate (IV-14)
To a solution of (3-(methoxycarbonyl) phenyl)boronic acid III-4 (1.10 mmol), methyl
3-bromo-2-cyanobenzoate II-9 (1.0 mmol), CsF (3.0 mmol), and Pd(dppf)C12 (0.02 mmol) in 8 mL of dioxane is added 2 mL of H2O. The mixture is stirred at 110°C under N2 overnight. The reaction mixture is filtered and concentrated, then extracted with ethyl acetate. The organic layer is dried over Na2SO4, filtered and concentrated. The product is purified by column chromatography (petroleum ether : ethyl acetate) to yield title product IV-14.
Step-2·. 3,3'-bis(hydroxymethyl)-[l,r-biphenyl]-2-carbonitrile (IV-15)
To a solution of LiBH4 (5—10 mmol) in MeOH (6 mL) is added dimethyl 2-cyano[l,l'-biphenyl]-3,3'-dicarboxylate IV-14 (1.0 mmol). The resulting mixture is stirred at rt. A TLC or LCMS analysis is conducted after 1 hour. When TLC/LCMS analyses indicate that the starting material is consumed, the solvent is removed in vacuo. The residue is dissolved in DCM (20 mL) and washed with H2O (10 mL). The organic layer is dried over Na2SO4, filtered and concentrated to afford the desired alcohol IV-15.
Step-3'. 3,3!-bis(((5-formyl-6-methoxypyridin-2-yl)oxy)methyl)-[l,r-biphenyl]-2carbonitrile, IV-16
Cesium carbonate (4.0 mmol, 2 eqv.), palladium(II) acetate (0.2 mmol, 0.1 eqv.), 2-di-tert-butylphosphino-2',4',6'-triisopropylbiphenyl (t-butyl Xphos) (0.4 mmol, 0.2 eqv.), 6-chloro-2-methoxynicotinaldehyde (2.6 mmol, 1.3 eqv.), and 3,3'-bis(hydroxymethyl)[l,l'-biphenyl]-2-carbonitrile IV-15 (1.0 mmol, 2N) are combined in a 25 mL round bottom flask equipped with a stir bar. Toluene (12 mL) is added and the mixture is
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Step-4'. follow the standard reductive amination procedures described for Example-16 to yield the title compound 1-30.
Example-20: 3,3'-bis[(4-{[(2-hydroxyethyl)amino]methyl}-3,5dimethoxyphenoxy)methyl] -[l,l'-biphenyl]-2-carbonitrile, 1-31.
Figure AU2017305399A1_D0104
IV-15
Figure AU2017305399A1_D0105
h2n^oh eqv.
NaBH3CN, EtOH
Step-3
Figure AU2017305399A1_D0106
Step-1'. 3,3!-bis(bromomethyl)-[l,l'-biphenyl]-2-carbonitrile, IV-17
To a solution of 3,3'-bis(hydroxymethyl)-[l,r-biphenyl]-2-carbonitrile IV-15 (1.0 mmol) in 40 mL of dichloromethane is added PBr3 (2.0 mmol) dropwise at 0°C. The mixture is stirred at 0°C overnight. It is quenched with water. The mixture is extracred with di chloromethane. The organic layer is dried over Na2SO4, filtered and concentrated. The product is purified by column chromatography (petroleum ether/ethyl acetate) to give the desired product IV-17.
Step-2'. 3,3'-bis((4-formyl-3,5-dimethoxyphenoxy)methyl)-[l,l'-biphenyl]-2carbonitrile, IV-18
A mixture of 3,3'-bis(bromomethyl)-[l,r-biphenyl]-2-carbonitrile, IV-17 (1.0 mmol),
4-hydroxy-2,6-dimethoxybenzaldehyde (2.17 mmol) and K2CO3 (2.5 mmol) in 7 mL of DMF is stirred at room temperature (or at 60-80 °C) for 16 h. The mixture is concentrated and extracted with ethyl acetate. The organic layer is dried over Na2SO4, filtered and concentrated. The crude product is subjected purification by normal column chromatography or reversed phase HPLC to yield the desired product IV-18.
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Step-3'. 3,3'-bis[(4-{[(2-hydroxyethyl)amino]methyl}-3,5dimethoxyphenoxy)methyl] -[l,r-biphenyl]-2-carbonitrile, 1-31.
Figure AU2017305399A1_D0107
To a solution of aldehyde IV-18 (1.0 mmol) in MeOH (30 mL) is added 2aminoethanol (4.0 mmol) and acetic acid (0.5 mmol). After stirring for 30 minutes, NaBH3CN(4.0 mmol) is added and the mixture is stirred for 16 hours at room temperature. The reaction is quenched with the water, and extracted with AcOEt. The combined organic layers is dried over anhydrious Na2SO4 and concentrated. The residue is purified by silica gel column (dichloromethane/ MeOH) to give the title compound 1-31.
The following compounds, 1-32—1-49 (in Table 1) are prepared in the same mannar as Example 18-20 from appropriate aldehyde intermediates and amines to provide the title compounds.
The compounds listed in Table 1 are prepared using methods similar to those described for the preparation of 1-1 to 1-31.
Table 1
C|mI. ID Chemical names Structure
1-1 2-({[4-({3'-[(4-{[(2- hydroxyethyl)amino]methyl}-2methy lphenoxy )methyl] -2,2'-dimethy l-[ 1,1 biphenyl]-3-yl}methoxy)-3- methy lpheny 1] methy 1} amino)ethan-1 -ol Xi J XX U °
1-2 2-{[(4-{[3-({4-[(azetidin-l-yl)methyl]-2methy lphenoxy } methy 1)-2chloropheny 1] methoxy } -3 methy lpheny l)methy ljamino }ethan-1 -ol >TX a ΌΧ
1-3 1 - [(4 - {[3 -({ 4- [(azetidin-1 -y l)methy 1] -2 methylphenoxy } methy 1)-2chloropheny 1] methoxy } -3 methy lpheny l)methy 1] azetidine ΓΝ'Χ'Χ Cl 3
1-4 (2S)-l-{[4-({3'-[(4-{[(2S)-2- carboxypiperidin-l-yl]methyl}-3,5dimethoxyphenoxy)methyl]-2,2'-dimethyl[l,l'-biphenyl]-3-yl}methoxy)-2,6dimethoxyphenyl]methyl}piperidine-2carboxylic acid COOH i Xji i FiX^nXi οΛι COOH O^
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1-5 (2S)-l-{ [4-({3'-[(4-{ [(2- hydroxyethyl)amino]methyl}-3,5dimethoxyphenoxy)methyl]-2,2'-dimethyl- [ 1, Γ-bipheny 1]-3 -yl}methoxy )-2,6dimethoxyphenyl]methyl}piperidine-2carboxylic acid COOH °x
1-6 2-({[4-({3'-[(4-{[(2- hydroxyethyl)amino]methyl}-3,5dimethoxyphenoxy)methyl]-2,2'-dimethyl[l,r-biphenyl]-3-yl}methoxy)-2,6dimethoxypheny l]methyl}amino)ethan-1 -ol ' η ι Ατν~°
1-7 l-{[4-({3'-[(4-{[(2hydroxyethyl)amino]methyl}-2methy lphenoxy )methyl] -2,2'-dimethy l-[ 1, 1 'biphenyl]-3-yl}methoxy)-2- methoxy phenyl] methyl }azetidin-3 -ol HO—Vk 1 M
1-8 2-{[(6-{ [3-(5-( [(5-{ [(2- hy dro xy ethy 1) amino ] me thy 1} -6 methoxypyridin-2-yl)oxy]methyl}-4methylthiophen-3-yl)-2methylphenyl]methoxy}-2-methoxypyridin-3y l)methyl] amino }ethan-1 -ol h fv « θχ
1-9 2-({[4-({4-[(4-{[(2hydroxyethyl)amino]methyl} -3 methoxyphenoxy) methyl]-3- methylthiophen-2-yl}methoxy)-2methoxyphenyljmethyl} amino)ethan-1 -ol
1-10 2-( { 4- [(azetidin-1 -y l)methy 1] -2 methylphenoxy }methyl)-6-{ [4-(( [(4oxoazetidin-2- y l)m ethyl] am ino } m ethy l)phenoxy ] m ethyl} benzonitrile
1-11 N-(2-{ [(4- {[3 -({4- [(azetidin-1 -yl)m ethyl] 2 -m ethylphenoxy } m ethyl) -2 cy anophenyljmethoxy } pheny l)m ethyl] amino }ethyl)acetamide Η Ν ^Νχ^Ν-^Χί^χ III Ο [A
1-12 2-( { 4- [(azetidin-1 -y l)methy 1] -2 methylphenoxy }methyl)-6-[(4-{ [(2hydroxyethyl)amino]methyl}phenoxy)meth yljbenzonitrile
1-13 2-( { 4- [(azetidin-1 -y l)methy 1] -2 - m ethylphenoxy } m ethy 1) -6 - ({ 4 - [(azetidin- 1 -yl)methyl]phenoxy } methyl)benzonitrile
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1-14 2-({[4-({4-[(4-{[(2hydroxyethyl)amino]methyl}-3,5dim ethoxyphenoxy )m ethyl]-1 H-indol-1 yl}methyl)phenyl]methyl}amino)ethan-l-ol
1-15 2-({[4-({l-[(4-{[(2- hydroxyethyl)amino]methyl}phenyl)m ethyl ] - 1 H-indazol-4-yl}methoxy)-2,6dimethoxyphenyl]methyl}amino)ethan-l-ol
1-16 2,6-bis[(4-{[(2hydroxyethyl)amino]methyl} -3 methoxy phenoxy )methyl]benzonitrile ηο'-^ν/χ^ϊ1 I|I ^V^n^oh H L £ X £ £ H
1-17 N-[(4-{[3'-({4[(cyclopropylamino)methyl]-3,5dim ethoxy phenoxy } m ethyl) -2,21 -dim ethy 1[1,1 '-biphenyl]-3 -yl]methoxy }-2,6dimethoxyphenyl)methyl]cyclopropanamin e v
1-20 N-[(4-{[3'-({4[(cyclopropylamino)methyl]-3,5dim ethoxy phenoxy } m ethyl) -2,21 -dim ethy 1[1,1 '-biphenyl]-3 -yl]methoxy }-2,6dimethoxyphenyl)methyl]cyclopropanamin e
1-19 2-[({6-[(3'-{[(5-{[(2- hydroxyethyl)amino]methyl}pyridin-2yl)oxy]methyl}-2,2'-dimethyl-[l,l'biphenyl] -3 -yl)methoxy]pyridin-3 yl}methyl)amino]ethan-l-ol iu'°
1-20 (3R)-l-{[4-({3'-[(4-{[(3R)-3hy droxypyrrolidin-1 -y l]methy 1} -2 methylphenoxy )methyl]-2,2'-dimethyl[l,l'-biphenyl]-3-yl}methoxy)-3methylphenyl]methyl }pyrrolidin-3 -ol
1-21 (2R,4R)-l-{[4-({3'-[(4-{[(2R,4R)-2carboxy-4-hy droxypyrrolidin-1 -yl]methyl} 2-m ethylphenoxy )methyl]-2,2'-dimethyl[l,l'-biphenyl]-3-yl}methoxy)-3methylphenyl]methyl} -4hydroxypyrrolidine-2-carboxylic acid c> V OH h° A i ΎΊΑ > 1 U 0H ho4o
1-22 2-[({6-[(3'-{[(5-{[(2hydroxyethyl)amino]methyl}-6methoxypyridin-2-yl)oxy]methyl}-2,2'dim ethyl- [1,1 '-biphenyl]-3 -yl)m ethoxy] -2methoxypyridin-3-yl}methyl)amino]ethanl-ol , nVn-^h
1-23 N-{2-[({6-[(3'-{[(5-{[(2- acetamidoethyl)amino]methyl}-6methoxypyridin-2-yl)oxy]methyl}-2,2'dim ethyl- [1,1 '-biphenyl]-3 -yl)m ethoxy] -2methoxypyridin-3 yl}methyl)amino]ethyl}acetamide > H H
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1-24 (2R,4R)-l-({6-[(3'-{[(5-{[(2R,4R)-2carboxy-4-hydroxypyrrolidin-1 -yl]methyl} 6-methoxypyridin-2-yl)oxy]methyl}-2,2'dim ethyl- [1,1 '-biphenyl]-3 -yl)m ethoxy] -2methoxypyridin-3-yl}methyl)-4hydroxypyrrolidine-2-carboxylic acid HO J X τ 1 ο. 'VOH X OH
1-25 (2R,4R)-4-hydroxy-l-({6-[(3'-{[(5-{[(2hydroxyethyl)amino]methyl}-6methoxypyridin-2-yl)oxy]methyl}-2,2'dim ethyl- [1,1 '-biphenyl]-3 -yl)m ethoxy] -2methoxypyridin-3-yl}methyl)pyrrolidine-2carboxylic acid HO (χ Cl Τ 1[ ο / Ο V OH OH
1-26 2-({[4-({3'-[(4-{[(2hydroxyethyl)amino]methyl}-3,5dim ethoxyphenoxy )methyl]-2'-m ethyl-[l,l'biphenyl] -3 -yl }methoxy)-2,6dimethoxyphenyl]methyl}amino)ethan-l-ol HO' H jOl X ^n~oh Τ
1-27 2-[({4-[(2-{3-[(4-{ [(2- hydroxyethyl)amino]methyl}-3,5- dim ethoxyphenoxy )methyl]-2- m ethylphenyl } py ridin-4 -y l)m ethoxy ] -2,6 diinethoxyphenyl}methyl)amino]ethan-l-ol HO' 1 0. X/X ο X ώ ο < 1
1-28 2-[({4-[(2-{3-[(4-{ [(2- hydroxyethyl)amino]methyl}-3,5- dim ethoxyphenoxy )methyl]-2- m ethylphenyl } py ridin-4 -yl)m ethoxy ] -2,6 diinethoxyphenyl}methyl)amino]ethan-l-ol HO' 1 H °' XX X X ^n~oh °
1-29 2-({[4-({2'-chloro-3'-[(4-{[(2- hydroxyethyl)amino]methyl}-2methylphenoxy)m ethyl] -[ 1, Γ-biphenyl] -3 yl}methoxy)-3- methylphenyl]methyl} amino)ethan-1 -ol Ηθ~ 1 0. X/X ο XX CI ό ο < 1
1-30 3,3'-bis({[(5-{[(2hydroxyethyl)amino]methyl}-6methoxypyridin-2-yl)oxy]methyl})-[l. 1 biphenyl] -2 -carbonitrile HO 1C Ν III υ X ---OH
1-31 3,3'-bis[(4-{[(2hydroxyethyl)amino]methyl}-3,5dim ethoxyphenoxy )methyl]-[l. 1 biphenyl] -2 -carbonitrile HO H°' ο ( 1C Ν III υ X ---OH Τ
1-32 3,3'-bis({[(5-{[(2hydroxyethyl)amino]methyl}pyridin-2y l)oxy Jmethy 1})- [ 1,1 '-biphenyl] -2 carbonitrile HO' H Ν III υ co ^n^OH H
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1-33 3,3 '-bis({4-[(azetidin-1 -yl)methyl]-3,5 dim ethoxyphenoxy }methyl)-[ 1,1 'biphenyl] -2 -carbonitrile .A N II Ό A0' A 1 NO
1-34 3,3 '-bis( { 4- [(3 -hydroxy azetidin-1 yl)methyl]-3,5- dim ethoxyphenoxy }methyl)-[ 1,1 'biphenyl] -2 -carbonitrile aA N II aA zA o / o —>H 1
1-35 3,3'-bis({[3,5-dimethoxy-4-({[(4oxoazetidin-2- y l)m ethyl] am ino } m ethy l)phenoxy ] m ethyl}) -[1,1 '-biphenyl] -2 -carbonitrile A A A N II v o < ζΑ 1
1-36 3,3'-bis[(4-{[(2hydroxyethyl)amino]methyl}-2m ethy lphenoxy)m ethyl] -[ 1, Γ-biphenyl] -2carbonitrile H0—Ά CA a N '1' u ^O^ An—°H Ji H
1-37 N-{2-[({6-[(2'-cyano-3'-{[(5-{[(2- acetamidoethyl)amino]methyl}-6methoxypyridin-2-yl)oxy]methyl} -[ 1, Γbipheny 1] -3 -yl)m ethoxy] -2 methoxypyridin-3 yl}inethyl)amino]ethyl}acetamide |j H H /oHh o ( A N III u % A H I—Y
1-38 2 - [(4- {[(2 -hydroxy ethy l)ainino]methyl} 3,5 -dim ethoxy phenoxy )m ethyl] -6- {4- [(4{[(2-hydroxyethyl)amino]methyl} -3,5 dim ethoxyphenoxy )methyl]pyridin-2yl}benzonitrile 1 H°H0-A A o < A N '1' u > o ΊζΑ° ?
1-39 2 - [(4- {[(2 -hydroxy ethy l)amino]methyl} -2 methylphenoxy )m ethyl]-6-{4-[(4-{ [(2hydroxyethyl)amino]methyl}-2methylphenoxy)methyl]pyridin-2yl}benzonitrile H0—Ά A a N '1' u
1-40 2 - {[(5 - {[(2 -hydroxy ethy l)amino]methyl} -6methoxypyridin-2-yl)oxy]methyl}-6-(4{[(5-{ [(2-hydroxyethyl)amino]methyl}-6methoxypyridin-2-yl)oxy]methyl}pyridin- 2 -y l)benzonitrile Ho— A /°Λ^ o < A N '1' u /°A o < jT^°H
1-41 2-[({4-[(2-{3-[(4-{[(2- hydroxyethyl)amino]methyl}-2methylphenoxy)methyl]-2- m ethylphenyl }pyridin-4-yl)m ethoxy] -3- m ethylphenyl }methyl)amino]ethan-1 -ol H ho~n^ ca A ό XA ^o— A—OH 1] H
1-42 3,3'-bis({[(5-{[(2- hydroxyethyl)amino]methyl}pyridin-2yl)oxy]methyl})-[l,l'-biphenyl]-2,2'dicarbonitrile H ho^N^ A a II N N '1' u -^A An—°H JI H
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1-43 3,3'-bis[(4-{[(2- hydroxyethyl)amino]methyl}-2methylphenoxy)m ethyl] -[ 1, Γ-biphenyl] 2,2' -die arbonitrile HO'*' Η i a ill N N '1' y o' z^ 0H H
1-44 3,3'-bis[(4-{[(2- hydroxyethyl)amino]methyl}phenoxy)meth y 1] - [ 1, Γ-bipheny 1] -2.2 '-die arbonitrile HO'*' H f a ill N N '1' y o' z^ 0H H
1-45 5,5'-bis[(4-{[(2- hydroxyethyl)amino]methyl}-2metliylphenoxy)methyl]-[3,3'-bipyridine]4,4' -die arbonitrile HO'*' H f (I s 1 N '1' yr N o' Ά z^ °H H
1-46 2-[({4-[(3-{5-[(4-{ [(2- hydroxyethyl)amino]methyl}-3,5dim ethoxyphenoxy )methyl]-4methy lthiophen-3 -yl } -2 methylphenyl)inetlioxy]-2,6dimethoxyphenyl}methyl)amino]ethan-l-ol HO^ 1 H0 o / v 'O'' 0 t S' ?
1-47 2-[({4-[(4-{3-[(4-{[(2- hydroxyethyl)amino]methyl}-2methylphenoxy)methyl]-2-methylphenyl}- 3 -methylthiophen-2-yl)methoxy]-3 methylphenyl }methyl)amino]ethan-1 -ol HO''*' H a0· O'' if N y h
1-48 2-({[4-({5'-[(4-{[(2hydroxyethyl)amino]methyl}-2methylphenoxy )methyl]-4,4'-dim ethylbi '-bithiophene] -5 -yl}methoxy)-3 m ethyl phony 1 |m ethyl} amino)ethan-1 -ol HO^ H ___N^· a°- xy y ~o 0 ,__/OH Z^N H
1-49 2-({[4-({5'-[(4-{[(2hydroxyethyl)amino]methyl}-2methylphenoxy)in ethyl] -1,1 '-dimethyl- 1 Η, 1Ή- [2,2 '-bipyrrole] -5 -y 1 }methoxy )-3 methylphenyl]methyl} amino)ethan-1 -ol HO^ H ___N^· a°- jry N 1 o' 0 ,__/OH Z^N H
Example-21: 5-[(5-{[3'-({5-[(5-cyanopyridin-3-yl)methoxy]-4-{ [(2-hydroxyethyl) amino]methyl}-2-methylphenoxy}methyl)-2,2'-dimethyl-[l,r-biphenyl]-3-yl]methoxy}-2{[(2-hydroxyethyl)amino]methyl}-4-methylphenoxy)methyl]pyridine-3-carbonitrile, 1-50.
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Figure AU2017305399A1_D0108
Figure AU2017305399A1_D0109
IV-2 h2n^0h eqv.
NaBH3CN, EtOH Step-3
Step-L·. 4,4'-(((2,2'-dimethyl-[l,r-biphenyl]-3,3'-diyl)bis(methylene))bis(oxy))bis(2hydroxy-5 -methylbenzaldehyde), IV-19.
A mixture of 2,4-dihydroxy-5-methylbenzaldehyde (2.2 mmol), triphenylphosphine (2.2 mmol) and (2,2'-dimethyl-[l,r-biphenyl]-3,3'-diyl)dimethanol (1.0 mmol) is dissolved in dry tetrahydro furan (14 mL) and cooled to 0 °C. Diisopropyl azodicarboxylate (2.2 mmol) in tetrahydrofuran (14 mL) is added dropwise. The resulting yellow solution is allowed to slowly warm to room temperature overnight. Solvent is removed by rotary evaporator. The crude is purified with an eluent (hexanes:ethyl acetate) on a silica gel column to afford the desired product, IV-19.
Step-2'. 5,5'-((((((2,2'-dimethyl-[l,r-biphenyl]-3,3'-diyl)bis(methylene))bis(oxy))bis(6-formyl-4-methyl-3,l-phenylene))bis(oxy))bis(methylene))dinicotinonitrile, IV-20
Cesium carbonate (3.0 mmol), 4,4'-(((2,2'-dimethyl-[l,r-biphenyl]-3,3'diyl)bis(methylene))bis(oxy))bis(2-hydroxy-5-methylbenzaldehyde) (1.0 mmol) and 5(chloromethyl)nicotinonitrile (4.0 mmol) are stirred at 75 °C for 3 hours in dimethyl formamide(5 mL). The reaction is filtered and concentrated. The residue is purified with a mixture of hexane:ethyl acetate on a silica gel column to afford the desired product IV-20.
Step-3·. 5-[(5-{[3'-({5-[(5-cyanopyridin-3-yl)methoxy]-4-{[(2-hydroxyethyl)amino]methyl}-2-methylphenoxy}methyl)-2,2'-dimethyl-[l,l'-biphenyl]-3-yl]methoxy}-2-{[(2hydroxyethyl) amino]methyl}-4-methylphenoxy)methyl]pyridine-3-carbonitrile, 1-50.
To a solution of aldehyde IV-20 (1.0 mmol) in MeOH (30 mL) is added 2aminoethanol (4.0 mmol) and acetic acid (0.5 mmol). After stirring for 30 minutes,
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NaBH3CN(4.0 mmol) is added and the mixture is stirred for 16 hours at room temperature. The reaction is quenched with the water, and extracted with ethyl acetate. The combined organic layers are dried over anhydrious Na2SO4 and concentrated. The residue is purified by silica gel column (eluent: DCM/ MeOH) to give the title compound 1-50.
Example-22: 5-[(5-{[3'-({5-[(5-cyanopyridin-3-yl)methoxy]-4-(hydroxymethyl)-2methylphenoxy}methyl)-2,2'-dimethyl-[l,r-biphenyl]-3-yl]methoxy}-2-(hydroxymethyl)-4methylphenoxy)methyl]pyridine-3-carbonitrile, 1-54.
Figure AU2017305399A1_D0110
5,5'-((((((2,2'-dimethyl-[l,r-biphenyl]-3,3'-diyl)bis(methylene)) bis(oxy))bis(6fomiyl-4-niethyl-3,l-phenylene))bis(oxy))bis(methylene))dinicotinonitrile, IV-20 (1.0 mmo) is dissolved in ethanol (15 mL), and NaBH4 (2.4 mmol) is added in small portions over 30 min to the stirred solution with ice cooling. The resulting solution is then stirred for
4-6 h at room temperature and a NaOH (1 M, 15 mL) solution was added to quench the reaction. The ethanol is removed by evaporation, the aqueous phase is extracted with ethyl acetate three times and the combined organic phase was washed with NaOH (1.0 M, 15 mL), water (15 mL) and brine (15 mL) and dried over anhydrous Na2SO4, filtered and concentrated. The residue was purified by a silica gel column chromatography (eluent:
ethyl acetate/petroleum ether) to yield the title molecule 1-54.
Example-23: 5-{[4-chloro-5-({2-cyano-3'-[(4-{[(2-hydroxyethyl)amino] methyl}2-methylphenoxy)methyl]-[l,r-biphenyl]-3-yl}methoxy)-2-{[(2-hydroxy ethyl) amino]methyl}phenoxy] methyl}pyridine-3-carbonitrile, 1-59.
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Figure AU2017305399A1_D0111
CN
Figure AU2017305399A1_D0112
Step-1
Pd(dppf)CI2, KOAc dioxane, 110 °C
Figure AU2017305399A1_D0113
Figure AU2017305399A1_D0114
CS2CO3, DMF, 75°C, 2h
Step-3
Figure AU2017305399A1_D0115
Figure AU2017305399A1_D0116
IV-23
Figure AU2017305399A1_D0117
Figure AU2017305399A1_D0118
Figure AU2017305399A1_D0119
Intermediate III-8 is prepared by the literature procedure described in Tetrahedron,
69(16), 3465-3474, 2013.
Figure AU2017305399A1_D0120
TBDMSCI
4-DMAP, THF
0°C, 24h, rt
Figure AU2017305399A1_D0121
Figure AU2017305399A1_D0122
Pd(dppf)CI2, KOAc dioxane, 110 °C
Figure AU2017305399A1_D0123
Step-1 Step-2
Step-1: 3'-(((tert-butyldimethylsilyl)oxy)methyl)-3-(hydroxymethyl)-[l,T biphenyl]-2-carbonitrile, 11-10.
To a solution of tert-butyldimethyl((3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2 yl)benzyl)oxy)silane III-8 (1.10 mmol), 2-bromo-6-(hydroxymethyl) benzonitrile (1.1 g, 1.0 mmol), KOAc (2.0 mmol), and Pd(dppf)C12 (0.07 mmol) in 8 mL of dioxane is added 2 mL of H2O. The mixture is stirred at 110°C under N2 overnight. The reaction mixture is filtered and concentrated, then extracted with ethyl acetate. The organic layer is dried over
Na2SO4, filtered and concentrated. The product is purified by column chromatography (petroleum ether : ethyl acetate) to give a desired product 11-10.
Step-2: 3'-(((tert-butyldimethylsilyl)oxy)methyl)-3-((2-chloro-4-formyl-5hydroxyphenoxy) methyl)-[ 1,1 !-biphenyl]-2-carbonitrile, IV-22.
A mixture of 5-chloro-2,4-dihydroxybenzaldehyde (1.1 mmol), triphenylphosphine (1.1 mmol) and 3!-(((tert-butyldimethylsilyl)oxy)methyl)-3
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PCT/US2017/045185 (hydroxymethyl)-[l,r-biphenyl]-2-carbonitrile, 11-10 (1.0 mmol) is dissolved in dry tetrahydro furan (7 mL) and cooled to 0 °C. Diisopropyl azodicarboxylate (1.1 mmol) in tetrahydrofuran (7 mL) is added dropwise. The resulting yellow solution is allowed to slowly warm to room temperature overnight. Solvent is removed by rotary evaporator. The crude is purified with an eluent (hexanes:ethyl acetate) on a silica gel column to afford the desired product, IV-22.
Step-3: 5-((5-((3'-(((tert-butyldimethylsilyl)oxy)methyl)-2-cyano-[l,l'-biphenyl]-3yl) methoxy)-4-chloro-2-formylphenoxy)methyl)nicotinonitrile, IV-23.
Cesium carbonate (1.5 mmol), 3'-(((tert-butyldimethylsilyl)oxy) methyl)-3-((2chloro-4-fomiyl-5-hydroxyphenoxy)methyl)-[l,r-biphenyl]-2-carbonitrile, IV-23 (1.0 mmol) and 5-(chloromethyl)nicotinonitrile (2.0 mmol) are stirred at 75 °C for 3 hours in dimethyl formamide(5 mL). The reaction is filtered and concentrated. The residue is purified with a mixture of hexane:ethyl acetate on a silica gel column to afford the desired product IV-23.
Step-4: 5-((4-chloro-5-((2-cyano-3'-(hydroxymethyl)-[l,r-biphenyl]-3yl)methoxy)-2-formyl phenoxy )methyl)nicotinonitrile, IV-24.
To a solution of 5-((5-((3'-(((tert-butyldimethylsilyl)oxy)methyl)-2-cyano-[l,rbiphenyl]-3-yl) methoxy)-4-chloro-2-formylphenoxy)methyl)nicotinonitrile, IV-23 (1.0 mmol) in THF (5 mL) is added tetrabutylammonium fluoride in THF (IM solution in THF, 2 mL, 2.0 mmol) drop wise at 0 °C. The reaction mixture is stirred at rt for 1 h and is diluted with H2O (10 mL) and extracted with EtOAc (3 κ 20 mL). The combined organic layer is dried over Na2SO4 and was concentrated under reduced pressure. The crude product was purified by column chromatography (silica gel, eluent hexane/EtOAc 30:70) to afford desired product IV-24.
Λϊβρ~5: 5-((4-chloro-5-((2-cyano-3'-((4-formyl-2-methylphenoxy )methyl)-[l,l'biphenyl]-3-yl)methoxy)-2-formylphenoxy)methyl)nicotinonitrile, IV-25.
A mixture of 4-hydroxy-3-methylbenzaldehyde (1.1 mmol), triphenylphosphine (1.1 mmol) and 5-((4-chloro-5-((2-cyano-3'-(hydroxymethyl)-[l,r-biphenyl]-3yl)methoxy)-2-formylphenoxy)methyl)nicotinonitrile, IV-24 (1.0 mmol) is dissolved in dry tetrahydrofuran (7 mL) and cooled to 0 °C. Diisopropyl azodicarboxylate (1.1 mmol) in tetrahydrofuran (7 mL) is added dropwise. The resulting yellow solution is allowed to slowly warm to room temperature overnight. Solvent is removed by rotary evaporator. The crude is purified with an eluent (hexanes:ethyl acetate) on a silica gel column to afford the desired product, IV-25.
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Step-6: 5-{[4-chloro-5-({2-cyano-3'-[(4-{[(2-hydroxyethyl)amino]methyl}-2methyl phenoxy) methyl]-[l,l'-biphenyl]-3-yl}methoxy)-2-{[(2hydroxyethyl)amino]methyl} phenoxy] methyl }pyridine-3-carbonitrile, 1-59.
A mixture of aldehyde IV-25 (1.0 mmol, 2N), 2-aminoethan-l-ol (6 mmol, 3 eqv.) and AcOH (10 mmol, 5 eqv.) in DMF (10 mL) is stirred at RT for 4-16 h. Then sodium cyanoborohydride (6 mmol, 3 eqv.) is added and the mixture is stirred at RT until reductive amination is complete (typically overnight). The crude is purified via reverse phase preparative HPLC to give the title compound 1-59.
Example 24: 5-[(4-chl oro-2-{ [(2-hydroxy ethyl)amino]methyl }-5-({3'-[(4-{ [(2hydroxyethyl) amino]methyl}-3,5-dimethoxyphenoxy)methyl]-2-methyl-[l,l'-biphenyl]-3yljmethoxy) phenoxy)methyl]pyridine-3-carbonitrile, 1-69.
The compound 1-69 can be synthesized by Method A shown below::
Figure AU2017305399A1_D0124
Figure AU2017305399A1_D0125
Pd(dppf)CI2, KOAc dioxane, 110 °C
Step-1
Figure AU2017305399A1_D0126
Step-2
Figure AU2017305399A1_D0127
Step-1-6 in Method A', follow the stardard procedures described for Example-23 to yield the tiltle compound 1-69.
The compound 1-69 can be synthesized by Method B shown below:
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Figure AU2017305399A1_D0128
Figure AU2017305399A1_D0129
Step-1
ΡΡπ3, DIAD THF, O’Ctort
Figure AU2017305399A1_D0130
Figure AU2017305399A1_D0131
Figure AU2017305399A1_D0132
Pd(dppf)CI2, KOAc dioxane, 110 °C
Step-3
Figure AU2017305399A1_D0133
Preparation of intermediate III-9:
Figure AU2017305399A1_D0134
Figure AU2017305399A1_D0135
Figure AU2017305399A1_D0136
Pd(dppf)CI2, KOAc dioxane, 110 eC
Figure AU2017305399A1_D0137
AU steps in MethodB: follow the stardard procedures described for Example-23 to yield the tiltle compound 1-69.
The compounds listed in Table 2 are prepared using methods similar to those described for the preparation of 1-50,1-54,1-59 and 1-69.
Table 2
Cpd. ID Chemical names Structure
5-[(5-{[3'-({5-[(5-cyanopyridin-3- Ν' r
yl)methoxy]-4-{ [(2-
hydroxy ethy l)amino]methyl}-2- Αχ 1 At
1-50 me thy lphenoxy } methy 1) -2,2 '-dimethy 1[1,1 '-biphenyl] -3 -y 1] methoxy } -2-{ [(2 - ¥
hy dro xy ethy 1) amino ] me thy 1}-4 - HCK·
methy lphenoxy )methyl]pyridine-3 - ¥
carbonitrile Λ
cn^n
2-({[4-({3'-[(4-{[(2- T
hy dro xy ethy l)amino] methy 1} -2 -methy 1-
5-[(pyridin-3- /A i A
1-51 yl)methoxy]phenoxy)methyl]-2,2'dimethyl-[ 1,1 '-biphenyl] -3 -yl}methoxy)- w Ay Γ H
5 -methy 1-2 - [(py ridin-3 - HO^
y l)methoxy ] pheny 1] methy 1} amino)ethan
-l-ol A
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1-52 5-[(4-chloro-5-{[3'-({2-chloro-5-[(5cyanopyridin-3-yl)methoxy]-4-{ [(2hydroxyethyl)amino]methyl}phenoxy }m ethyl)-2,2'-dimethyl-[l,l'-biphenyl]-3yl]methoxy}-2-{[(2- hy droxy ethy l)amino]methyl}phenoxy)m ethy 1] py ridine-3 -carbo nitriIc HO'^' .=+ 3 Cl T1 7
χΑ νγ ο < 1
5-[(5-{[3'-({5-[(5-cyanopyridin-3- Ν' CN
y l)methoxy ] -2 -methy 1-4 - % |
[(me thy lamino)methy 1] phenoxy } methy 1) I Π ρ r Y
1-53 -2,2 '-dimethyl- [ 1, Γ-bipheny 1] -3 - iV' A3 ΥΎ ^0' τ Ji
y 1] methoxy }-4-methyl-2- A 1
[(methy lamino)methy 1] phenoxy )methy 1] 0.
py ridine-3 -carbonitrile A cn^n
5-[(5-{[3'-({5-[(5-cyanopyridin-3- Ν' ? .CN
yl)methoxy]-4-(hydroxymethyl)-2- 1
methy lphenoxy } methy 1) -2,2 '-dimethy 1- I p Γ r OH
1-54 [1,1 '-biphenyl] -3 -y 1] methoxy } -2- γγ ΪΎ Α' τ Ji
(hy droxy methy 1)-4 - HO. 1
methy lphenoxy )methyl]py ridine-3 -
carbonitrile CN λ
5-[(3-{[3'-({5-[(5-cyanopyridin-3- CN
yl)methoxy] -2-methylphenoxy } methy 1)- Λ
1-55 2,2'-dimethy l-[ 1, Γ-bipheny 1] -3 yl] methoxy}-4- ( br 3
methy lphenoxy )methyl]py ridine-3 - Γ 0
carbonitrile CN'
5-[(5-{[3'-({5-[(5-cyanopyridin-3- Ν' CN
yl)methoxy]-4-(hydroxymethyl)-2- %
methylphenoxy } methy 1)-2 -methy l-[ 1,1'- I Pi Λ Ρ OH
1-56 biphenyl] -3 -y 1] methoxy } -2 - (i ΑγΑ, II γν τ
(hy droxy methy 1)-4 - HC\ r
methy lphenoxy )methyl]py ridine-3 - °Ί
carbonitrile CN Λ An
3,3'-bis({5-[(5-cyanopyridin-3- c r xo
yl)methoxy]-4-{ [(2- I Ρ CN
1-57 hy dro xy ethy 1) amino ] me thy 1}-2 - η if u T
methylphenoxy } methy l)-[ 1,1 '-biphenyl] - HO' / CN Μ 1
2,2'-dicarbonitrile %
λ
CNX Pn
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1-58 5-[(5-{[2-cyano-3'-({5-[(5-cyanopyridin3-yl)methoxy]-4-{ [ty- hy dro xy ethy 1) amino ] me thy 1}-2methylphenoxy }methy l)-[ 1,1 '-biphenyl] 3-yl]methoxy}-2-{[(2hydroxyethyl)amino]methyl}-4methy lphenoxy )methyl]py ridine-3 carbonitrile HO^ cn^n Cl CN ύ n-Vcn
5-{ [4-chloro-5-({2-cyano-3'-[(4-{ [(2hy dro xy ethy 1) amino ] me thy 1}-2 - n^cn Y
1-59 methylphenoxy )methyl] -[1,1 '-biphenyl] -
3-yl}methoxy)-2-{[(2- CN -s-OH
hydroxy ethy l)amino]methyl}phenoxy]m ,0.,. u
ethy 1 }py ridine-3 -carbonitrile HO^ jQ u 0 T Cl
5-({4-chloro-5-[(2-cyano-3'-{ [(5-{[(2hy dro xy ethy 1) amino ] me thy 1} py ridin-2 - N^fCN Y
1-60 y l)oxy]methyl}-[ 1,1'-biphenyl]-3 -
yl)methoxy]-2-{ [(2- CN —OH
hydroxy ethy l)amino]methyl}phenoxy }m A, JUx Y
ethy l)py ridine-3 -carbonitrile HcU H JU u 0 I Cl
5-{ [5-({2-cyano-3'-[(4-{ [(2hy dro xy ethy 1) amino ] me thy 1}-2 - u
1-61 methylphenoxy )methyl] -[1,1 '-biphenyl] - 3-yl}methoxy)-2-{[(2- CN O -s-OH
hydroxy ethy l)amino]methyl}phenoxy]m ,0., u
ethy 1 }py ridine-3 -carbonitrile HO^ H jQ u
3'-[(4-{[(2-
hy dro xy ethy 1) amino ] me thy 1}-2 - V
methylphenoxy )methyl]-3-[(4-{ [(2- J
1-62 hy dro xy ethy l)amino]methyl}-3[(pyridin-3- -Os -Os CN ./n
y l)methoxy ]phenoxy)methy 1] -[ 1,1'- H u o
biphenyl] -2-carbonitrile HO^ υυ
5-{[5-({2'-cyano-3'-[(4-{[(2- nCN
hy dro xy ethy 1) amino ] me thy 1}-2 - V
methylphenoxy )methyl] -[1,1 '-biphenyl] - J
1-63 3-yl}methoxy)-2-{[(2- -\-OH
hy dro xy ethy 1) amino ] me thy 1}-4 - 1 -Q Co
methylphenoxy]methyl}pyridine-3- H Λ -Os____ ΓΥ 'o^Y
carbonitrile HO^ CN IlsY 1
5-{ [5-({2-cyano-3'-[(4-{ [(2- nCN
hy dro xy ethy 1) amino ] me thy 1}-2 - V
methylphenoxy )methyl] -[1,1 '-biphenyl] - J
1-64 3-yl}methoxy)-2-{[(2- -s-OH
hy dro xy ethy 1) amino ] me thy 1}-4 - 1 -Q CN Co
methylphenoxy]methyl}pyridine-3- H Λ -Os____ O ^o^Y
carbonitrile HO^ 1
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1-65 5-{[5-({2'-cyano-3'-[(4-{[(2- hy droxy ethy l)amino]methyl}-2methy lphenoxy )methyl] -[1,1 '-biphenyl] 3-yl}methoxy)-2-{[(2- hy dro xy ethy 1) amino ] me thy 1} -4 methy lphenoxy]methyl}pyridine-3carbonitrile HO^ H N^fCN ί
Οχ Τ [ X/' x^J ,fy Cl
5-({4-chloro-5-[(2-cyano-3'-{ [(5-{[(2hy dro xy ethy l)amino]methyl}pyridin-2- T CN
1-66 y l)oxy Jmethy l}-[ 1, Γ-bipheny 1]-3yl)methoxy]-2- CN A
[(cyclopropylamino)methyl]phenoxy}m ethy l)py ridine-3 -carbonitrile H _Ν. ί /Οχ. JU Αγ J H
HO' Cl
2-({[4-({3'-[(4-{[(2-
hy dro xy ethy l)amino]methyl}-3- V
[(pyridin-3- L
1-67 yl)methoxy]phenoxy)methyl]-2,2'dimethyl-[ 1,1 '-biphenyl] -3 -yl}methoxy)- η - Cl O χ^-/0Η
3-methylphenyl]methyl}amino)ethan-l- H Γτ Τ Ί] A/
ol HO^ χ-jJ
5-{[5-({2'-cyano-3'-[(4-{[(2- rf
hy dro xy ethy 1) amino ] me thy 1}-2 -
methy lphenoxy )methyl] -[1,1 '-biphenyl] - L
1-68 3-yl}methoxy)-2-{[(2-
hy droxy ethy l)amino] methy 1}-4 - I A JO
methylphenoxy]methyl}pyridine-3- H ΙιΎ -Οχ___, Ο^Ρ^χ, I I
carbonitrile HO^ χ-Νχ/ Μ CN k 1
5-[(4-chloro-2-{[(2- hy droxy ethy l)amino] methy 1}-5-({ 3' - [(4 - N^fCN Ϊ
{[(2-hydroxyethyl)amino]methyl}-3,5- ko
1-69 dimethoxy phenoxy )methy 1] -2 -methy 1- Jy X^/°H
[1,1'-biphenyl]-3- 1 Οχ -°χ/ LX AO o-LL H
y 1} methoxy )phenoxy )methy 1] py ridine - x-nP υ I X U 0 T Cl
3-carbonitrile HO^ τ Οχ
5-[(2-{[(2-hydroxyethyl)amino]methyl}- rf
5-({3'-[(4-{[(2-
hy dro xy ethy 1) amino ] me thy 1}-2 - L
1-70 methy lphenoxy )methyl]-2,2'-dimethyl-
[ 1, Γ-bipheny 1]-3-yl}methoxy )-4- 1 A JO
methy lphenoxy )methyl]py ridine-3 - Η ΐίΎ -Οχ___/ AA, T 1 AP
carbonitrile HO^ Μ x^C 1
5-[(2-{[(2-hydroxyethyl)amino]methyl}- 5-({3'-[(4-{[(2- N^fCN Ϊ
hydroxy ethy l)amino]methyl}-3,5- ko
1-71 dimethoxyphenoxy )methyl]-2,2'- Jy X^/°H
dimethyl-[ 1,1 '-biphenyl] -3 -yl}methoxy)- 1 Οχ -°χ/ LX AO o-LL H
4-methy lphenoxy )methyl]py ridine-3 carbonitrile HO^ •Ο Q T I x^-L 0 T
Οχ
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2-({[4-({3'-[(4-{[(2hy droxy ethy l)amino] me thy 1} -2 -methy 15-[(pyridin-3yl)methoxy]phenoxy)methyl]-2,2'dimethyl-[ 1,1 '-biphenyl] -3 -yl}methoxy )2,6dimethoxyphenyl]methyl}amino)ethanl-ol
Figure AU2017305399A1_D0138
Figure AU2017305399A1_D0139
Example 25: 2-[({2-[3'-(6-{[(2-hydroxyethyl)amino]methyl}-[l,2,4]triazolo[l,5-
a]pyridin-2-yl)-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -y 1 ] - [ 1,2,4]tri azolof 1,5-a]pyridin-6- yl }methyl)amino]ethan-1 -ol, 1-73.
The compound 1-73 can be synthesized via the route shown in the scheme below.
Figure AU2017305399A1_D0140
ho—
AcOH, NaBH3CN
Figure AU2017305399A1_D0141
Example 26: 2-[({2-[3'-(6-{[(2-hydroxyethyl)amino]methyl}imidazo[l,2-a]pyridin2-yl)-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -yl]imidazo[ 1,2-a]pyridin-6-yl [methyl) amino]ethan-1 ol, 1-74.
The compound 1-74 can be synthesized via the route shown in the scheme below.
Figure AU2017305399A1_D0142
JohnPhos
Figure AU2017305399A1_D0143
CuBr2, AcOEt
80°C,2h
Figure AU2017305399A1_D0144
KF, Pd(OAc)2 THF, rt, >24h
Figure AU2017305399A1_D0145
OH
Figure AU2017305399A1_D0146
Example 27: 2-[({2-[3'-(6-{[(2-hydroxyethyl)amino]methyl}-l,3-benzoxazol-2-yl)2,2'-dimethyl-[l,T-biphenyl]-3-yl]-l,3-benzoxazol-6-yl}methyl)amino]ethan-l-ol, 1-75.
The compound 1-75 can be synthesized via the route shown in the scheme below.
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Figure AU2017305399A1_D0147
Figure AU2017305399A1_D0148
Dess-Martin periodinane
Figure AU2017305399A1_D0149
Example 28: 3-(5-( [(2-hydroxy ethyl)amino]methyl}-1,3-benzoxazol-2-yl)-3'-[(4{[(2-hy droxy ethyl)amino]methyl} -2-methylphenoxy)methyl]-[ 1,1 '-biphenyl]-2-carbonitrile, 1-87
The compound 1-87 can be synthesized via the route shown in the scheme below:
Figure AU2017305399A1_D0150
Figure AU2017305399A1_D0151
The compounds listed in Table 3 are prepared using methods similar to those described for the preparation of 1-73,1-74 and 1-87.
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Table 3
Cp<l. ID Chemical names Structure
1-73 2-[({2-[3'-(6-{ [(2- hy dro xy ethy 1) amino ] me thy 1} [l,2,4]triazolo[l,5-a]pyridin-2-yl)-2,2'dimethy 1- [1,1 '-biphenyl] -3 -y 1] [ 1,2,4]triazolo[ 1,5-a]py ridin-6y l}methy l)amino]ethan-1 -ol n_n-O HO^N
1-74 2-[({2-[3'-(6-{[(2- hy droxy ethy l)amino] me thy 1} imidazo [1,2- a]py ridin-2-y 1)-2,2'-dimethyl-[ 1,1 '-biphenyl] -3 yl]imidazo[l,2-a]pyridin-6- y l}methy l)amino]ethan-1 -ol __/OH / ho^n rYUCV
1-75 2-[({2-[3'-(6-{[(2- hy droxy ethy l)amino]methyl}-l,3-benzo xazol-2yl)-2,2'-dimethyl-[l,l'-biphenyl]-3-yl]-l,3benzoxazol-6-yl}methyl)amino]ethan-l-ol r===N ^/OH
1-76 2-({2-[3'-(5-{[(2-hydroxyethyl)amino]methyl}l,3-benzoxazol-2-y 1)-2,2'-dimethyl-[l, Γbipheny l]-3-y 1]-1,3-benzo xazol-5yl}amino)ethan-l-ol OH HO
1-77 2-[({2-[3'-(5-{[(2- hy droxy ethy l)amino]methyl}-l,3-benzothiazol2-y 1)-2,2'-dimethy l-[ 1, Γ-bipheny 1] -3 -y 1] -1,3 benzothiazol-5 -y 1 }methy l)amino] ethan-1 -ol OH HO
1-78 2-[({2-[3'-(6-{[(2- hy droxy ethy l)amino]methyl}-l,3-benzothiazol2-y 1)-2,2'-dimethy l-[ 1, Γ-bipheny 1] -3 -y 1] -1,3 benzothiazol-6-yl}methyl)amino]ethan-l-ol OH HO
1-79 3,3'-bis(6-{[(2-hydroxyethyl)amino]methyl}- [l,2,4]triazolo[l,5-a]pyridin-2-yl)-[l,l'biphenyl]-2,2'-dicarbonitrile /=\N'Ny^N^0H N. 4. Λ \ H H /—\ / N
1-80 3,3'-bis(6-{[(2-hydroxyethyl)amino]methyl}l,3-benzoxazol-2-yl)-[l,l'-biphenyl]-2,2'dicarbonitrile m /=Vv°'Y^^N^0H N. 4. Z>—J J H h N
1-81 2-[({2-[3'-(7-{[(2- hy dro xy ethy 1) amino ] me thy 1} - [1.2.4] triazolo[l,5-a]pyridin-2-yl)-2,2'dimethv 1- [1,1 ’-biphenyl] -3 -y 1] - [1.2.4] triazolo [ 1,5-a]py ridin-7 yl}methyl)amino]ethan- l-ol y-u) / N=ro HO
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1-82 2-{[(8-chloro-2-{3'-[(4-{[(2- hy droxy ethy l)amino]methyl}-2methy lphenoxy jmethyl] -2,2'-dimethy l-[ 1, Γbipheiiyl]-3-yl}-[l,2,4]triazolo[l,5-a]pyridin-6y l)methyl] amino }ethan-1 -ol 01
1-83 3-(8-chloro-6-{[(2hydroxyethyljaminojmethyl}[l,2,4]triazolo[l,5-a]pyridin-2-yl)-3'-[(4-{[(2hydroxyethyl)amino]methyl}-2- methy lphenoxy jmethyl] -[1,1 '-biphenyl] -2 carbonitrile vTnAJ h HO^ \ Cl
1-84 3'-[(4-{[(2-hydroxyethyl)amino]methyl}-3,5dimethoxyphenoxy jmethyl] -3 -(6-{ [(2hydroxyethyljaminojmethyl}- [l,2,4]triazolo[l,5-a]pyridin-2-yl)-[l,rbipheny 1] -2-carbonitrile
1-85 3' - [(4 - {[(2 -hy droxy ethy ljamino] methyl} -2 methylphenoxy )methyl]-3-(6-{ [(2hy droxy ethy ljaminojmethyl}[l,2,4]triazolo[l,5-a]pyridin-2-yl)-[l,l'bipheny 1] -2-carbonitrile
1-86 2-{[(2-{3'-[(4-{[(2- hy droxy ethy l)amino]methyl}-2methy lphenoxy jmethyl] -2,2'-dimethy l-[ 1, Γbiphenyl]-3-yl}-l,3-benzoxazol-6- y ljmethy l]amino }ethan-1 -ol ^OH HO% J
1-87 3-(5-{[(2-hydroxyethyl)amino]methyl}-l,3benzoxazol-2-yl)-3'-[(4-{[(2- hy droxy ethy l)amino]methyl}-2methy lphenoxy jmethyl] -[1,1 '-biphenyl] -2 carbonitrile -OH HO-, /==\ .. HN^
1-88 2-{[(2-{3'-[(4-{[(2- hy droxy ethy l)amino]methyl}-2methy lphenoxy jmethyl] -2,2'-dimethy l-[ 1, Γbiphenyl]-3-yl}-l,3-benzoxazol-5- y ljmethy ljamino }ethan-1 -ol -OH Η0-Λ ΓΆ J
1-89 2-{[(2-{3'-[(4-{[(2- hy droxy ethy l)amino]methyl}-2methy lphenoxy jmethyl] -2,2'-dimethy l-[ 1, Γbiphenyl]-3-yl}-l,3-benzothiazol-5- y ljmethy ljamino }ethan-1 -ol CHXX
1-90 2-{[(2-{3'-[(4-{[(2- hy droxy ethy l)amino]methyl}-2methy lphenoxy jmethyl] -2,2'-dimethy l-[ 1, Γbiphenyl]-3-yl}-l,3-benzothiazol-6- y ljmethy ljamino }ethan-1 -ol /=\/s^^n^oh h
1-91 2-{[(2-{3'-[(4-{[(2- hy droxy ethy l)amino]methyl}-2methy lphenoxy jmethyl] -2,2'-dimethy l-[ 1, Γbiphenyl]-3-yl}-[l,2,4]triazolo[l,5-a]pyridin-6y ljmethy ljamino }ethan-1 -ol HO^nh Λλ
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1-92 3' - [(4 - {[(2 -hy droxy ethy l)amino] methyl} -2 methylphenoxy )methyl]-3-(6-{ [(2hy droxy ethy l)amino]methyl}[l,2,4]triazolo[l,5-a]pyridin-2-yl)-2'-methyl[1,1 '-biphenyl] -2 -carbonitrile
1-93 2-{[(4-chloro-2-{3'-[(4-{[(2- hy droxy ethy l)amino]methyl}-2- methy lphenoxy )methyl] -2,2'-dimethy l-[ 1, Γbipheny l]-3-yl}-l,3-benzo xazol-6- y l)methyl] amino }ethan-1 -ol “Λ ΖΛ ' ci NH /—V )
1-94 3-(4-chloro-6-{ [(Σ- ικάτο xy ethy l)amino]methyl}-l,3-benzo xazol-2yl)-3'-[(4-{[(2-hydroxyethyl)amino]methyl}-2methy lphenoxy )methy 1] -[1,1 '-biphenyl] -2 carbonitrile HO^ /FA V Cl
1-95 3-(5-{[(2-hydroxyethyl)amino]methyl}-l,3benzoxazol-2-yl)-3'-[(4-{ [(Σ- ικάτο xy ethy l)amino]methyl}-3,5dimethoxyphenoxy )methy 1] -[ 1, Γ-bipheny 1] -2carbonitrile CHXX.ii^oh
1-96 3-(4-chloro-6-{[(2- hy droxy ethy l)amino]methyl}-l,3-benzo xazol-2yl)-3'-[(4-{[(2-hydroxyethyl)amino]methyl}-2methy lphenoxy )methyl]-2'-methyl-[ 1, Γbipheny 1] -2-carbonitrile o-aju h HO^ /““C V Cl
1-97 2-[({2-[3'-(5-{[(2hydroxyethyl)amino]methyl}-2H-indazol-2-yl)2,2'-dimethy l-[ 1, Γ-bipheny 1]-3-y l]-2H-indazol- 5 -y 1} methy l)amino] ethan-1 -ol __/OH HO^N \^2=N /
1-98 2-[({2-[3'-(6-{[(2hydroxyethyl)amino]methyl}-2H-indazol-2-yl)2,2'-dimethy l-[ 1, Γ-bipheny 1]-3-y l]-2H-indazol6-y l}methy l)amino] ethan-1 -ol __/OH HO^N f
1-99 2-[({2-[3'-(6-{[(2- hy droxy ethy l)amino] methy 1} imidazo [1,2b]pyridazin-2-yl)-2,2'-dimethyl-[l,l'-biphenyl]3 -y l]imidazo [ 1,2-b]pyridazin-6- y l}methy l)amino] ethan-1 -ol Ηο^Ν^θ=Ν
1-100 2-[({6-[3'-(2-{[(2- hydroxyethyl)amino]methyl}imidazo[l,2b][l,2,4]triazin-6-yl)-2,2'-dimethyl-[l,l'biphenyl]-3-yl]imidazo[l,2-b][l,2,4]triazin-2y l}methy l)amino] ethan-1 -ol ηο-Ά~~ν V /
1-101 2-[({2-[3'-(6-{[(2- hy droxy ethy l)amino] methy 1} imidazo [1,2a]pyrazin-2-yl)-2,2'-dimethyl-[l,l'-biphenyl]-3yl]imidazo[l,2-a]pyrazin-6- y l}methy l)amino] ethan-1 -ol ho^n /
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1-102 2-({ [4-({3-[2-(4-{ [(2- hy dro xy ethy l)ammo]methyl}pheiiyl)-7-methy 11,3 -benzoxazol-6-y 1] -2- methy lpheny 1} methoxy )-3- methy lpheny 1] methy 1} amino)ethan-1 -ol til T0H
1-103 2-({ [4-({3-[2-(3-{ [(2- hy dro xy ethy l)amino]methyl}phenyl)-7-methy 11,3 -benzoxazol-6-y 1] -2- methy lpheny 1} methoxy )-3- methy lpheny 1] methy 1} amino)ethan-1 -ol OH AN 1 A Al
1-104 2-{[(4-{6-[2-(4-{[(2- hy dro xy ethy l)amino]methyl}phenyl)-7-methy 11,3 -benzoxazol-6-y 1] -7-methyl- 1,3 -benzo xazol2-y l}pheny l)methyl] amino }ethan-1 -ol 1 ΤθΗ
1-105 2-{[(3-{6-[2-(3-{[(2- hy dro xy ethy l)amino]methyl}phenyl)-7-methy 11,3 -benzoxazol-6-y 1] -7-methyl- 1,3 -benzo xazol2-y l}pheny l)methyl] amino }ethan-1 -ol OH A+AVaA HOZ
1-106 2-[({2-[3'-(5-{ [(2- hy dro xy ethy l)amino] methy 1} -2,3 -dihy dro -1H isoindol-2-yl)-2,2'-dimethyl-[LΓ-biphenyl]-3yl]-2,3-dihydro-lH-isoindol-5- y l}methy l)amino]ethan-1 -ol __ __/OH
1-107 2-[({6-[3'-(3-{[(2- hy dro xy ethy l)amino] methy 1} -2 -methoxy 5H,6H,7H-pyrrolo[3,4-b]pyridin-6-yl)-2,2'dimethy 1- [1,1 '-biphenyl] -3 -y 1] -2 -metho xy5H,6H,7H-pyrrolo[3,4-b]pyridin-3y l}methy l)amino]ethan-1 -ol
The compounds disclosed herein, but not listed in Table 1-3 above, can be prepared in a similar manner as Compounds 1-1 to 1-105.
DOCKING EXPERIMENTS
Compounds 1-1 and 1-10 are selected as ligands to bind PD-L1 dimer. Figure 1 shows docking pose of compound 1-1 in PD-L1 dimer (Panel A). Figure 2 shows docking pose of compound 1-10 in PD-L1 dimer (Panel B)
Method of Docking: the protein model was built based on PDB structure of PD-L1 dimer (pdb code: 5j8o) in which Y56 conformation in B chain was rebuilt to allow access to both sides of the ligand core structure. Ligands were docked using rdock and docking poses were selected based on docking score and visual inspection.
Figure legend: the ligand binding site at the dimer interface was displayed in an orientation in which chain A and chain B were located above and below the ligand respectively. Ligands were displayed in ball and stick representation. Surfaces were created surrounding the ligand binding site and were clipped to allow ligand to be better displayed.
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As shown in Figures 1 and 2, compounds 1-1 and 1-5 dock well with PD-L1 dimer. In both cases, hydrophobic channel accommodates the designed novel core scaffold in the center; two pseudo symmetrical side chains attached to the core are extended to either side of the dimer interface. It is believed that such designed inhibitors can effectively induce/stabilize PD-L1 dimer formation, therefore potently disrupting PD-1/PD-L1 proteinprotein and CD80/PD-L1 protein-protein interaction. Other compounds disclosed herein are believed to exhibit the same properties in docking experiments. Thus, these compounds can also be potent and selective inhibitors of the PD-1/PD-L1 protein/protein and CD80/PD-L1 protein/protein interactions.
BIOLOGICAL ASSAY
The activity of the compounds of Formula (I) to inhibit PD-1/PD-L1 protein-protein interaction can be readily investigated using biochemical and cellular assays well accepted in the field.
Homogenous time-resolved fluorescence (HTRF) binding assay:
The ability of the designed compounds to physically disrupt PD-1/PD-L1 interaction was measured by HTRF binding assay. The interaction of PD-1 and PD-L1 was assessed using soluble, purified preparations of the extracellular domains of the two proteins. For PD1, the tag is the Fc portion of immunoglobulin (PD-l-Ig). For PD-L1, it is the 6 histidine motif (PD-Ll-His). All required fusion proteins with desired tags were obtained from commercial sources. HTRF assay buffer consists of lxPBS supplemented with 0.1% (w/v) bovine serum albumin and 0.05% (v/v) Tween-20. For the binding assay, PD-Ll-His (30 nM final) and PD1-Ig (10 nM final) in HTRF assay buffer were pre-incubated at RT for 30 min, followed by addition of inhibitors and incubated for another 30 min. HTRF detection was achieved using Tb cryptate-labeled anti-Ig antibody (1 nM final) and d2-labeled anti-His antibody (20 nM final). Antibodies were diluted in HTRF assay buffer and dispensed on top of the binding reaction. The reaction mixture was allowed to equilibrate for 60 min at RT before the resulting signal (665nm/620nm ratio) was obtained using an EnVision fluorometer. Final DMSO concentration in the solution is 0.2%. Additional binding assays can be established between PD- 1-Ig and PD-L2-His or CD80-His/PD-Ll -Ig, in a similar format. The IC50 of the designed compounds in disrupting PD-1/PD-L1 interaction will be expected to fall in the range of 0.01 nM to 100 uM, depending on the corresponding structure-activity relationship. IC50 determination was performed by fitting the curve of percent control activity versus the log of the inhibitor concentration using the GraphPad Prism 5.0 software.
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Compounds of the present disclosure, as exemplified in Examples 1-6, showed IC50 values in the following ranges: A: IC50 <0.1 uM; B: 0.1 uM < IC5o<l-O uM; C: 0.1 uM < IC50 < 25 uM
Data obtained for the representative compounds using the PD-1/PD-L1 homogenous time- resolved fluorescence (HTRF) binding assay described in Example 29 is shown in Table 4.
Table 4
Gpd ID PD-l/PD-LI HTRFICm
1-1 A
1-2 C
1-3 c
1-4 B
1-5 A
1-6 A
T-cell activation assay:
The ability of the designed compounds to functionally inhibit PD-1/PD-L1 interaction on a cellular level can be measured by T-cell activation assay. Human peripheral blood mononuclear cells (PBMC) can be isolated from blood huffy coats by commercial kits available. CD4+ T cells can be isolated with CD4 enrichment kit as per the manufacturer's instructions. Mouse Ig capture beads can be coated with anti-CD3, anti-CD28 and PD-L1 Fc fusion by incubation under rotation at 4°C. CD4+ T cells can be cultured in 96-well plates together with coated beads, with or without designed compounds at varying concentrations for 3 days at 37°C in RPMI1640 Glutamax I supplemented with 4% human AB serum. Culture supernatant can be removed to measure cytokine expression (e.g. IFNs, IL-2) by ELISA, DELFIA or Luminex technology. The amount of cytokine can be determined by comparing with a standard curve of known amounts of human cytokines. The remaining T cells can be quantified by standard cell proliferation/survival assays (e.g. Thymidine incorporation, CellTiter-Glo) according to manufacturer’s instructions. Potent inhibitor compounds will disrupt PD-L1 protein binding to PD-1 on the T cell surface, thus resulting in enhanced cytokine expression and promotion of T cell proliferation/activity.
Mixed lymphocyte reaction:
The ability of the designed compounds to functionally inhibit endogenous PD-l/PDL1 interaction and promote T cell activity can be measured by mixed lymphocyte reaction
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The compounds of Formula (I) possess activity as inhibitors of the PD-1/PD-L1 interaction, and therefore, may be used in the treatment of diseases dependent on or associated with the PD-1/PD-L1 interaction. Through inhibition of the PD-1/PD-L1 interaction, the compounds of the present disclosure may be utilized to treat infectious diseases such as Hepatitis C, as well as multiple forms of cancer.
It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention cover the modifications and variations of this invention provided they come within the scope of the appended claims and their equivalents.

Claims (4)

  1. WHAT IS CLAIMED IS:
    1.
    A compound having Formula I:
    (I), or a pharmaceutical acceptable salt thereof, wherein:
    A is a bivalent arene or a bivalent heteroarene;
    Ring B and Ring B’ are independently a 6-membered aromatic hydrocarbon ring, a 6membered heterocyclic ring, a 9- to 10-membered aromatic hydrocarbon ring, or a 9- to 10membered heterocyclic ring;
    Y and Y’ are independently null (direct bond), -CHRi-, -CH2-CH2-, -NRi-, -0-, -
    R3 and R’3 are independently H, SO2NH2, SCFNRjRr,, SO2NHR- '· OCH3 , cHtNRjRg, or CH2NHR7, wherein R5 and R6 are independently H, Ci-6 alkyl, C3. 8 cycloalkyl, or heteroaryl or R5 and R6 form a C3-8 cycloalkyl, heterocyclyl, or heteroaryl ring, and R7 is H, aryl, heteroaryl, acetyl, CH2CH2OH, CH2CH2NHCOCH3, C3-C8 alkyl carboxylic acid, C3-C8 alkyl amide, C3-C8 alkyl alcohol, -CH2-Ar, or -CH2-heterocyclyl; and
    R4, R’4, Z, and Z’ are independently H, halogen, CHF2, CF3, CN, Ci-6 alkyl, Ci-6 alkoxy, aryl, or heteroaryl.
  2. 2. The compound of claim 1, wherein the Formula (I) is represented by following
    R·
    R.
    Formula (II):
    Ζ'
    Ring B and Ring B’ are independently a 6-membered aromatic hydrocarbon ring or a
    6-membered heterocyclic ring;
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    Xi, X’i, X2, and X’2 are independently CR2, C=O or NR2, and R2 is H, Me, CN, halogen, OMe, CHF2, CF3, Ci-6 alkoxyl, C3-7 cycloalkyl, C3-7 heterocyclyl, or OCH2Ar;
    X3, and X’3 are independently C or N; and
    Y and Y’ are independently -CHRi-, -CH2-CH2-, -NRi-, -0-, -OCH2-, -CH2O-, SCH2-, -CH2S-, -SOCH2-, -CH2SO-, or -SO2CH2-, and Ri is H, Ci_6 alkyl, or C3.6 cycloalkyl.
  3. 3. The compound of claim 1, wherein the Formula (I) is represented by following
    Formula (III): z' (III),
    Ring B and Ring B’ are independently a 9- to 10-membered aromatic hydrocarbon ring or a 9- to 10 -membered heterocyclic ring;
    Xi, and XT are independently CR2, C=O or NR2, and R2 is H, Me, CN, halogen, OMe, CHF2, CF3, Ci-6 alkoxyl, C3-7 cycloalkyl, C3-7 heterocyclyl, or OCH2Ar;
    X2, and X’2, X3, and X’3 are independently C or N;
    U and U’ are independently C and N;
    V and V’ are O, S, -(CH2)2-, -CR’-, or -N=; and
    Y and Y’ are independently null (direct bond), -CHRi-, -CH2-CH2-, -NRi-, -0-, 0CH2-, -CH20-, -SCH2-, -CH2S-, -SOCH2-, -CH2SO-, or -SO2CH2-, and Ri is H, Ci_6 alkyl, or C3-6 cycloalkyl.
    WO 2018/026971
    PCT/US2017/045185 and Ra’ are independently COR’, OMe, halogen, Ci-6 alkyl, C2-6alkynyl, Ci-6 cycloalkyl, CN, CF3; CH2CF3; R’ is Ci-6 alkyl; Rb and Rb’ are independently Ci-6 alkyl, C2_6 alkynyl, Ci-6 cycloalkyl or alkyl halogen; and V is -(CH2)2-, -CR’-, or -N=.
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    6.
    The compound of claim 1, 2, 3, or 4, wherein CH2NHR7 is are
    7.
    The compound of claim
    1, 2, or 3, wherein Ring B and Ring B’
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    Z
    8. The compound of claim 2, wherein Ring B and Ring B’ are independently
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    Z Z'
    R2 R2
    7 7
    7 7 7 7 7
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    10. The compound of claim 1, 2, or 3 wherein R3 and R’3 are the same, R4 and R’4 are the same, Y and Y’ are the same, Z and Z’ are the same, or Ring B and Ring B’ are the same; or R3 and R’3 are the same, R4 and R’4 are the same, Y and Y’ are the same, Z and Z’ are the same, and Ring B and Ring B’ are the same.
    12. The compound of claim 1, 2, or 3, wherein the compound is 2-({[4-({3'-[(4{[(2-hydroxyethyl)amino]methyl} -2-methylphenoxy)methyl]-2,2'-dimethyl-[ 1,1 '-biphenyl]-
    3-yl}methoxy)-3-methylphenyl]methyl}amino)ethan-l-ol; 2-{[(4-{[3-({4-[(azetidin-lyl)methyl]-2-methylphenoxy}methyl)-2-chlorophenyl]methoxy}-3methylphenyl)methyl]amino}ethan-l-ol; l-[(4-[[3-({4-[(azetidin-l-yl)methyl]-2methylphenoxy}methyl)-2-chlorophenyl]methoxy}-3-methylphenyl)methyl]azetidine; (2S)l_{[4-({3'-[(4-{[(2S)-2-carboxypiperidin-l-yl]methyl}-3,5-dimethoxyphenoxy)methyl]-2,2'dimethyl-[l,r-biphenyl]-3-yl}methoxy)-2,6-dimethoxyphenyl]methyl}piperidine-2carboxylic acid; (2S)-l-{[4-({3'-[(4-{[(2-hydroxyethyl)amino]methyl}-3,5dimethoxyphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}methoxy)-2,6dimethoxyphenyl]methyl}piperidine-2-carboxylic acid; 2-({[4-({3'-[(4-{[(2hydroxyethyl)amino]methyl}-3,5-dimethoxyphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-
    3-yl}methoxy)-2,6-dimethoxyphenyl]methyl}amino)ethan-l-ol; l-{[4-({3'-[(4-{[(2hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3yl}methoxy)-2-methoxyphenyl]methyl}azetidin-3-ol; 2-{[(6-{[3-(5-{[(5-{[(2hydroxyethyl)amino]methyl}-6-methoxypyridin-2-yl)oxy]methyl}-4-methylthiophen-3-yl)-2methylphenyl]methoxy}-2-methoxypyridin-3-yl)methyl]amino}ethan-l-ol; 2-({[4-({4-[(4{[(2-hydroxyethyl)amino]methyl}-3-methoxyphenoxy) methyl]-3-methylthiophen-2yl}methoxy)-2-methoxyphenyl]methyl}amino)ethan-1 -ol; 2-({4-[(azetidin-1 -yl)methyl]-2methylphenoxy }methyl)-6-{ [4-( {[(4-oxoazetidin-2yl)methyl]amino}methyl)phenoxy]methyl}benzonitrile; N-(2-[[(4-{[3-({4-[(azetidin-l
    WO 2018/026971
    PCT/US2017/045185 yl)methyl]-2-methylphenoxy)methyl)-2-cyanophenyl]methoxy) phenyl)methyl]amino)ethyl)acetamide; 2-({4-[(azetidin-l-yl)methyl]-2methylphenoxy)methyl)-6-[(4-{[(2hydroxyethyl)amino]methyl} phenoxy)methyl]benzonitrile; 2-({4-[(azetidin-l-yl)methyl]-2methylphenoxy} methyl)-6-([4-[(azetidin-l-yl)methyl]phenoxy} methyl)benzonitrile; 2-({[4({4-[(4-{ [(2-hydroxy ethyl)amino]methyl }-3,5-dimethoxyphenoxy)methyl]-lH-indol-lyl}methyl)phenyl]methyl}amino)ethan-l-ol; 2-({[4-({l-[(4-{[(2hydroxyethyl)amino]methyl)phenyl)methyl]-lH-indazol-4-yl)methoxy)-2,6dimethoxyphenyl]methyl}amino)ethan-l-ol; 2,6-bis[(4-{[(2-hydroxyethyl)amino]methyl}-3methoxyphenoxy)methyl]benzonitrile; N-[(4-{[3'-([4-[(cyclopropylamino)methyl]-3,5dimethoxyphenoxy}methyl)-2,2'-dimethyl-[l, r-biphenyl]-3-yl]methoxy )-2,6dimethoxyphenyl)methyl]cyclopropanamine; 4-{[({4-[(3'-{[3,5-dimethoxy-4-({[(4oxoazetidin-2-yl)methyl]amino)methyl)phenoxy]methyl}-2,2'-dimethyl-[l,r-biphenyl]-3yl)methoxy]-2,6-dimethoxyphenyl)methyl)amino]methyl}azetidin-2-one; 2-[({6-[(3'-{ [(5{[(2-hydroxyethyl)amino]methyl)pyridin-2-yl)oxy]methyl}-2,2'-dimethyl-[l,r-biphenyl]-3yl)methoxy]pyridin-3-yl)methyl)amino]ethan-l-ol; 2-[({6-[(3'-{[(5-{[(2hydroxyethyl)amino]methyl} -6-methoxypyridin-2-yl)oxy]methyl} -2,2'-dimethyl-[ 1, Γbiphenyl]-3-yl)methoxy]-2-methoxypyridin-3-yl}methyl)amino]ethan-l-ol; N-{2-[({6-[(3'{[(5-{[(2-acetamidoethyl)amino]methyl)-6-methoxypyridin-2-yl)oxy]methyl}-2,2'-dimethyl[1,1 '-biphenyl]-3 -yl)methoxy]-2-methoxypyridin-3 -yl }methyl)amino] ethyl} acetamide; (2R,4R)-l-({6-[(3'-{[(5-{[(2R,4R)-2-carboxy-4-hydroxypyrrolidin-l-yl]methyl}-6methoxypyridin-2-yl)oxy]methyl} -2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -yl)m ethoxy]-2methoxypyridin-3-yl)methyl)-4-hydroxypyrrolidine-2-carboxylic acid; (2R,4R)-4-hydroxy-l({6-[(3'-{[(5-{[(2-hydroxyethyl)amino]methyl)-6-methoxypyridin-2-yl)oxy]methyl}-2,2'dimethyl-[l,r-biphenyl]-3-yl)methoxy]-2-methoxypyridin-3-yl)methyl)pyrrolidine-2carboxylic acid; (3R)-l-{[4-({3'-[(4-{[(3R)-3-hydroxypyrrolidin-l-yl]methyl}-2methylphenoxy)methyl]-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -yl }methoxy)-3 methylphenyl]methyl)pyrrolidin-3-ol; (2R,4R)-l-{[4-({3'-[(4-{[(2R,4R)-2-carboxy-4hydroxypyrrolidin-1 -yl]methyl} -2-methylphenoxy)methyl]-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 yl)methoxy)-3-methylphenyl]methyl)-4-hydroxypyrrolidine-2-carboxylic acid; 2-({[4-({3'[(4-{[(2-hydroxyethyl)amino]methyl}-3,5-dimethoxyphenoxy)methyl]-2'-methyl-[l,l·biphenyl]-3-yl)methoxy)-2,6-dimethoxyphenyl]methyl)amino)ethan-l-ol; 2-[({4-[(2-{3-[(4{[(2-hydroxyethyl)amino]methyl)-3,5-dimethoxyphenoxy)methyl]-2-methylphenyl)pyridin-
  4. 4-yl)methoxy]-2,6-dimethoxyphenyl)methyl)amino]ethan-l-ol; 2-[({4-[(6-{3-[(4-{ [(2
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    PCT/US2017/045185 hy droxy ethyl)amino]methyl}-3,5-dimethoxyphenoxy)methyl]-2-methylphenyl}pyridin-2yl)methoxy]-2,6-dimethoxyphenyl}methyl)amino]ethan-l-ol; 2-(( [4-((2'-chloro-3'-[(4-( [(2hy droxy ethyl)amino]methyl} -2-methylphenoxy)methyl]-[ 1,1 '-biphenyl]-3 -yl }methoxy)-3 methylphenyl]methyl}amino)ethan-l-ol; 3,3'-bis[(4-([(2-hydroxyethyl)amino]methyl)-3,5dimethoxyphenoxy)methyl]-[l,r-biphenyl]-2-carbonitrile; 3,3'-bis(( [(5-( [(2hy droxy ethyl)amino]methyl }-6-methoxypyridin-2-yl)oxy]methyl})-[ 1,1 '-biphenyl]-2carbonitrile; 3,3'-bis(([(5-([(2-hydroxyethyl)amino]methyl)pyridin-2-yl)oxy]methyl))-[l,l·biphenyl]-2-carbonitrile; 3,3'-bis({4-[(azetidin-l-yl)methyl]-3,5-dimethoxyphenoxy(methyl)[l,r-biphenyl]-2-carbonitrile; 3,3'-bis((4-[(3-hydroxyazetidin-l-yl)methyl]-3,5dimethoxyphenoxy}methyl)-[l,r-biphenyl]-2-carbonitrile; 3,3'-bis(([3,5-dimethoxy-4-(([(4oxoazetidin-2-yl)methyl]amino}methyl)phenoxy]methyl})-[l,r-biphenyl]-2-carbonitrile;
    3,3'-bis[(4-{ [(2-hydroxy ethyl)amino]methyl}-2-methylphenoxy)methyl]-[l,r-biphenyl]-2carbonitrile; N-{2-[((6-[(2'-cyano-3'-{[(5-{[(2-acetamidoethyl)amino]methyl)-6methoxypyridin-2-yl)oxy]methyl}-[l,r-biphenyl]-3-yl)methoxy]-2-methoxypyridin-3yl}methyl)amino]ethyl}acetamide; 2-[(4-{[(2-hydroxyethyl)amino]methyl)-3,5dimethoxyphenoxy)methyl] -6- { 4-[(4- {[(2-hy droxy ethyl)amino]methyl )-3,5dimethoxyphenoxy)methyl]pyridin-2-yl(benzonitrile; 2-[(4-{[(2hydroxyethyl)amino]methyl)-2-methylphenoxy)methyl]-6-{4-[(4-{[(2hydroxyethyl)amino]methyl)-2-methylphenoxy)methyl]pyridin-2-yl(benzonitrile; 2-{[(5{[(2-hy droxy ethyl)amino]methyl)-6-methoxypyridin-2-yl)oxy]methyl )-6-(4-( [(5-{ [(2hydroxyethyl)amino]methyl}-6-methoxypyridin-2-yl)oxy]methyl}pyridin-2-yl)benzonitrile; 2-[({4-[(2-{3-[(4-{[(2-hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-2methylphenyl}pyridin-4-yl)methoxy]-3-methylphenyl}methyl)amino]ethan-l-ol; 3,3'bis({[(5-{[(2-hydroxyethyl)amino]methyl}pyridin-2-yl)oxy]methyl})-[l,r-biphenyl]-2,2'dicarbonitrile; 3,3'-bis[(4-{[(2-hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-[l,l·biphenyl]-2,2'-dicarbonitrile; 3,3'-bis[(4-([(2-hydroxyethyl)amino]methyl}phenoxy)methyl][l,r-biphenyl]-2,2'-dicarbonitrile; 5,5'-bis[(4-{[(2-hydroxyethyl)amino]methyl}-2methylphenoxy)methyl]-[3,3'-bipyridine]-4,4'-dicarbonitrile; 2-[((4-[(4-(3-[(4-([(2hy droxy ethyl)amino]methyl) -3,5 -dimethoxyphenoxy)m ethyl] -2-methylphenyl )-3methylthiophen-2-yl)methoxy]-2,6-dimethoxyphenyl)methyl)amino]ethan-l-ol; 2-[((4-[(4{ 3 - [(4- ([(2-hy droxy ethyl)amino]methyl) -2-methylphenoxy)m ethyl] -2-methylphenyl )-3methylthiophen-2-yl)methoxy]-3-methylphenyl)methyl)amino]ethan-l-ol; 2-(( [4-((5'-[(4{[(2-hy droxy ethyl)amino]methyl)-2-methylphenoxy)methyl]-4,4'-dimethyl-[3,3'bithiophene]-5-yl)methoxy)-3-methylphenyl]methyl)amino)ethan-l-ol; 2-(([4-((5'-[(4-([(2
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    PCT/US2017/045185 hy droxy ethyl)amino]methyl} -2-methylphenoxy)methyl]-1,1 '-dimethyl- 1H, 1 Ή-[2,2'bipyrrole]-5-yl}methoxy)-3-methylphenyl]methyl}amino)ethan-l-ol; 5-[(5-{[3'-({5-[(5cy anopyridin-3-yl)methoxy]-4-{ [(2-hy droxy ethyl)amino]methyl}-2-methylphenoxy}methyl)2,2'-dimethyl-[l,r-biphenyl]-3-yl]methoxy}-2-{ [(2-hy droxy ethyl)amino]methyl }-4methylphenoxy)methyl]pyridine-3-carbonitrile; 2-({[4-({3'-[(4-{[(2hydroxyethyl)amino]methyl}-2-methyl-5-[(pyridin-3-yl)methoxy]phenoxy)methyl]-2,2'dimethyl-[l,r-biphenyl]-3-yl}methoxy)-5-methyl-2-[(pyridin-3yl)methoxy]phenyl]methyl}amino)ethan-l-ol; 5-[(4-chloro-5-{[3'-({2-chloro-5-[(5cy anopyridin-3-yl)methoxy]-4-{ [(2-hy droxy ethyl)amino]methyl (phenoxy }methyl)-2,2'dimethyl-[l,r-biphenyl]-3-yl]methoxy}-2-{[(2hydroxyethyl)amino]methyl(phenoxy)methyl]pyridine-3-carbonitrile; 5-[(5-{[3'-({5-[(5cyanopyridin-3-yl)methoxy]-2-methyl-4-[(methylamino)methyl]phenoxy}methyl)-2,2'dimethyl-[l,r-biphenyl]-3-yl]methoxy}-4-methyl-2[(methylamino)methyl]phenoxy)methyl]pyridine-3-carbonitrile; 5-[(5-{[3'-({5-[(5cyanopyridin-3-yl)methoxy]-4-(hydroxymethyl)-2-methylphenoxy(methyl)-2,2'-dimethyl[l,r-biphenyl]-3-yl]methoxy}-2-(hydroxymethyl)-4-methylphenoxy)methyl]pyridine-3carbonitrile; 5-[(3-{[3'-({5-[(5-cyanopyridin-3-yl)methoxy]-2-methylphenoxy(methyl)-2,2'dimethyl-[l,r-biphenyl]-3-yl]methoxy}-4-methylphenoxy)methyl]pyridine-3-carbonitrile; 5[(5-{[3'-({5-[(5-cyanopyridin-3-yl)methoxy]-4-(hydroxymethyl)-2-methylphenoxy (methyl)2-methyl-[l,r-biphenyl]-3-yl]methoxy}-2-(hydroxymethyl)-4methylphenoxy)methyl]pyridine-3-carbonitrile; 3,3'-bis({5-[(5-cyanopyridin-3-yl)methoxy]-
    4-{ [(2-hy droxy ethyl)amino]methyl}-2-methylphenoxy}methyl)-[l,r-biphenyl]-2,2'dicarbonitrile; 5-({4-chloro-5-[(2-cyano-3'-{[(5-{[(2-hydroxyethyl)amino]methyl(pyridin-2yl)oxy (methyl(-[1,1 '-biphenyl] -3 -yl)methoxy ] -2- {[(2hydroxyethyl)amino]methyl(phenoxy(methyl)pyridine-3-carbonitrile; 5-{[5-({2-cyano-3'[(4-{[(2-hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-[l,r-biphenyl]-3yl(methoxy)-2-{[(2-hydroxyethyl)amino]methyl(phenoxy]methyl(pyridine-3-carbonitrile; 5{[4-chloro-5-({2-cyano-3'-[(4-{ [(2-hydroxy ethyl)amino]methyl(-2-methylphenoxy)methyl][1,1 '-biphenyl]-3 -yl (methoxy)-2- {[(2hydroxyethyl)amino]methyl(phenoxy]methyl(pyridine-3-carbonitrile; 3'-[(4-{[(2hydroxyethyl)amino]methyl(-2-methylphenoxy)methyl]-3-[(4-{[(2- hy droxy ethyl)amino]methyl }-3-[(pyri din-3-yl)methoxy]phenoxy)methyl]-[ l,l'-biphenyl]-2carbonitrile; 5-{[5-({2'-cyano-3'-[(4-{[(2-hydroxyethyl)amino]methyl(-2methylphenoxy)methyl]-[ 1,1 '-biphenyl]-3-yl (methoxy)-2-{ [(2-hydroxy ethyl)amino]methyl (80
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    4-methylphenoxy]methyl}pyridine-3-carbonitrile; 5-{[5-({2-cyano-3'-[(4-{[(2hydroxyethyl)amino]methyl} -2-methylphenoxy)methyl]-[ 1,1 '-biphenyl]-3 -yl}methoxy)-2{[(2-hydroxyethyl)amino]methyl}-4-methylphenoxy]methyl}pyridine-3-carbonitrile; 5-[(4chl oro-2- {[(2-hy droxy ethyl)amino]methyl }-5-[(3'-{[(5-{[(2hydroxyethyl)amino]methyl}pyridin-2-yl)oxy]methyl}-2,2'-dimethyl-[l,r-biphenyl]-3yl)methoxy]phenoxy)methyl]pyridine-3-carbonitrile; 5-({4-chloro-5-[(2-cyano-3'-{[(5-{[(2hy droxy ethyl)amino]methyl }pyridin-2-yl)oxy]methyl} -[ 1,1 '-biphenyl]-3 -yl)methoxy]-2[(cyclopropylamino)methyl]phenoxy}methyl)pyridine-3-carbonitrile; 5-{[5-({2-cyano-3'-[(4{[(2-hy droxy ethyl)amino]methyl}-3,5-dimethoxyphenoxy)methyl]-2'-methyl-[l,r-biphenyl]-
    3-yl }methoxy)-2-{ [(2-hydroxy ethyl)amino]methyl}-4-methylphenoxy]methyl}pyridine-3carbonitrile; 5-[(5-{[2-cyano-3'-({5-[(5-cyanopyridin-3-yl)methoxy]-4-{[(2hy droxy ethyl)amino]methyl}-2-methylphenoxy}methyl)-[l,r-biphenyl]-3-yl]methoxy}-2{[(2-hydroxyethyl)amino]methyl}-4-methylphenoxy)methyl]pyridine-3-carbonitrile; 2-({[4({3'-[(4-{ [(2-hydroxy ethyl)amino]methyl}-3-[(pyridin-3-yl)methoxy]phenoxy)methyl]-2,2'dimethyl-[ 1,1 '-biphenyl]-3-yl}methoxy)-3-methylphenyl]methyl}amino)ethan-1 -ol; 5-[(4chl oro-2-{[(2-hy droxy ethyl)amino]methyl}-5-({3'-[(4-{ [(2-hy droxy ethyl)amino]methyl}-3,5dimethoxyphenoxy)methyl]-2-methyl-[l,r-biphenyl]-3yl}methoxy)phenoxy)methyl]pyridine-3-carbonitrile; 5-[(2-{[(2hy droxy ethyl)amino]methyl}-5-({3'-[(4-{ [(2-hydroxy ethyl)amino]methyl }-2methylphenoxy)methyl]-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -yl }methoxy)-4methylphenoxy)methyl]pyridine-3-carbonitrile; 5-[(2-{[(2-hydroxyethyl)amino]methyl}-5({3'-[(4-{[(2-hydroxyethyl)amino]methyl}-3,5-dimethoxyphenoxy)methyl]-2,2'-dimethyl[l,r-biphenyl]-3-yl}methoxy)-4-methylphenoxy)methyl]pyridine-3-carbonitrile; 2-({[4-({3'[(4-{[(2-hydroxyethyl)amino]methyl}-2-methyl-5-[(pyridin-3-yl)methoxy]phenoxy)methyl]2,2'-dimethyl-[l, 1 '-biphenyl]-3-yl }methoxy)-2,6-dimethoxyphenyl]methyl }amino)ethan-1 -ol; 5 - {[(3 - {[(2-hy droxy ethyl)amino]methyl} -6- [(3'- {[(5 - {[(2-hy droxy ethyl)amino]methyl }-6methoxypyridin-2-yl)oxy]methyl}-2,2'-dimethyl-[l,r-biphenyl]-3-yl)methoxy]pyridin-2yl)oxy]methyl}pyridine-3-carbonitrile; 2-({[4-({3'-[(2-chloro-4-{[(2hy droxy ethyl)amino]methyl}-5-[(pyridin-3-yl)methoxy]phenoxy)methyl]-2,2'-dimethyl-[ 1,1'biphenyl]-3-yl}methoxy)-3-methylphenyl]methyl}amino)ethan-l-ol; 5-[(5-{[2-cyano-3'-({5[(5-cyanopyridin-3-yl)methoxy]-4-(hydroxymethyl)-2-methylphenoxy}methyl)-2'-methyl[l,r-biphenyl]-3-yl]methoxy}-2-(hydroxymethyl)-4-methylphenoxy)methyl]pyridine-3carbonitrile; 5-({2-[(azetidin-l-yl)methyl]-5-{[3'-({4-[(azetidin-l-yl)methyl]-5-[(5cyanopyridin-3-yl)methoxy]-2-methylphenoxy}methyl)-2-cyano-[l,r-biphenyl]-3
    WO 2018/026971
    PCT/US2017/045185 yl]methoxy}-4-methylphenoxy}methyl)pyridine-3-carbonitrile; 5-({2-[(azetidin-lyl)methyl]-5-{[3'-({4-[(azetidin-l-yl)methyl]-5-[(5-cyanopyridin-3-yl)methoxy]-2methylphenoxy}methyl)-2,2'-dimethyl-[l,r-biphenyl]-3-yl]methoxy}-4methylphenoxy}methyl)pyridine-3-carbonitrile; 2-[({2-[3'-(6-{[(2hydroxyethyl)amino]methyl} -[ 1,2,4]tri azolof 1,5-a]pyridin-2-yl)-2,2'-dimethyl-[ 1 ,Γbiphenyl]-3-yl]-[l,2,4]triazolo[l,5-a]pyridin-6-yl}methyl)amino]ethan-l-ol; 2-[({2-[3'-(6{[ (2-hy droxy ethyl)amino]methyl }imidazo[ 1,2-a]pyridin-2-yl)-2,2'-dimethyl-[ 1,1 '-biphenyl]-
    3-yl]imidazo[l,2-a]pyridin-6-yl}methyl)amino]ethan-l-ol; 2-[({2-[3'-(6-{[(2hydroxyethyl)amino]methyl}-l,3-benzoxazol-2-yl)-2,2'-dimethyl-[l,r-biphenyl]-3-yl]-l,3benzoxazol-6-yl}methyl)amino]ethan-l-ol; 2-({2-[3'-(5-{ [(2-hydroxyethyl)amino]methyl}-
    1,3 -benzoxazol-2-yl)-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -y 1 ] -1,3 -benzoxazol-5-yl} amino)ethan-
    1- ol; 2-[({2-[3'-(5-{[(2-hydroxyethyl)amino]methyl}-l,3-benzothiazol-2-yl)-2,2'-dimethyl[l,r-biphenyl]-3-yl]-l,3-benzothiazol-5-yl}methyl)amino]ethan-l-ol; 2-[({2-[3'-(6-{[(2hydroxyethyl)amino]methyl]-l,3-benzothiazol-2-yl)-2,2'-dimethyl-[l,r-biphenyl]-3-yl]-l,3benzothiazol-6-yl]methyl)amino]ethan-l-ol; 3,3'-bis(6-{ [(2-hy droxy ethyl)amino]methyl}- [l,2,4]triazolo[l,5-a]pyridin-2-yl)-[l,r-biphenyl]-2,2'-dicarbonitrile; 3,3'-bis(6-{[(2hydroxyethyl)amino]methyl}-l,3-benzoxazol-2-yl)-[l,r-biphenyl]-2,2'-dicarbonitrile; 2-(((2[3'-(7-{[(2-hydroxyethyl)amino]methyl}-[l,2,4]triazolo[l,5-a]pyridin-2-yl)-2,2'-dimethyl[l,r-biphenyl]-3-yl]-[l,2,4]triazolo[l,5-a]pyridin-7-yl}methyl)amino]ethan-l-ol; 2-{[(8- chl oro-2-{3'-[(4-{ [(2-hy droxy ethyl)amino]methyl]-2-methylphenoxy)methyl]-2,2'-dimethyl[l,r-biphenyl]-3-yl}-[l,2,4]triazolo[l,5-a]pyridin-6-yl)methyl]amino}ethan-l-ol; 3-(8chloro-6-{[(2-hydroxyethyl)amino]methyl}-[l,2,4]triazolo[l,5-a]pyridin-2-yl)-3'-[(4-{[(2hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-[l,r-biphenyl]-2-carbonitrile; 3'[(4- {[(2-hy droxy ethyl)amino]methyl} -3,5 -dimethoxyphenoxy)methyl] -3 -(6- {[(2hydroxyethyl)amino]methyl} -[ 1,2,4]triazolo[ 1,5-a]pyridin-2-yl)-[ 1,1 '-biphenyl]-2carbonitrile; 3'-[(4-{ [(2-hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-3-(6-{ [(2hy droxy ethyl)amino]methyl} -[ 1,2,4]triazolo[ 1,5-a]pyridin-2-yl)-[ 1,1 '-biphenyl]-2carbonitrile; 2-{ [(2-{3'-[(4-{ [(2-hydroxyethyl)amino]methyl]-2-methylphenoxy)methyl]-2,2'dimethyl-[l,r-biphenyl]-3-yl]-l,3-benzoxazol-6-yl)methyl]amino}ethan-l-ol; 3-(5-{[(2hy droxy ethyl)amino]methyl]-l,3-benzoxazol-2-yl)-3'-[(4-{ [(2-hy droxy ethyl)amino]methyl}-
    2- methylphenoxy)methyl]-[l,r-biphenyl]-2-carbonitrile; 2-{[(2-{3'-[(4-{[(2- hy droxy ethyl)amino]methyl]-2-methylphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}- l,3-benzoxazol-5-yl)methyl]amino}ethan-l-ol; 2-{[(2-{3'-[(4-{[(2hy droxy ethyl)amino]methyl]-2-methylphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}82
    WO 2018/026971
    PCT/US2017/045185
    1.3- benzothiazol-5-yl)methyl]amino)ethan-l-ol; 2-{[(2-{3'-[(4-{[(2- hy droxy ethyl)amino]methyl)-2-methylphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl)-
    1.3- benzothiazol-6-yl)methyl]amino)ethan-l-ol; 2-{[(2-{3'-[(4-{[(2- hy droxy ethyl)amino]methyl}-2-methylphenoxy)methyl]-2,2'-dimethyl-[l,r-biphenyl]-3-yl}- [l,2,4]triazolo[l,5-a]pyridin-6-yl)methyl]amino)ethan-l-ol; 3'-[(4-{[(2hydroxyethyl)amino]methyl}-2-methylphenoxy)methyl]-3-(6-{[(2- hy droxy ethyl)amino]methyl) -[ 1,2,4]tri azolof 1, 5-a]pyridin-2-yl)-2'-methyl-[ 1,1 '-biphenyl]-2carbonitrile; 2-{ [(4-chl oro-2-{3'-[(4-{ [(2-hy droxy ethyl)amino]methyl )-2methylphenoxy)methyl]-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 -yl) -1,3 -benzoxazol-6yl)methyl] amino ) ethan-1 -ol; 3 -(4-chloro-6- {[(2-hydroxyethyl)amino]methyl )-1,3benzoxazol-2-yl)-3'-[(4-{ [(2-hy droxy ethyl)amino]methyl)-2-methylphenoxy )methyl]-[l,l'biphenyl]-2-carbonitrile; 3-(5-{[(2-hydroxyethyl)amino]methyl)-l,3-benzoxazol-2-yl)-3'-[(4{[(2-hy droxy ethyl)amino]methyl )-3,5-dimethoxyphenoxy)methyl]-[l,r-biphenyl]-2carbonitrile; 3-(4-chloro-6-{[(2-hydroxyethyl)amino]methyl)-l,3-benzoxazol-2-yl)-3'-[(4{[(2-hy droxy ethyl)amino]methyl)-2-methylphenoxy)methyl]-2'-methyl-[l,r-biphenyl]-2carbonitrile; 2-[({2-[3'-(5-{[(2-hydroxyethyl)amino]methyl)-2H-indazol-2-yl)-2,2'-dimethyl[l,l'-biphenyl]-3-yl]-2H-indazol-5-yl)methyl)amino]ethan-l-ol; 2-[({2-[3'-(6-{[(2hy droxy ethyl)amino]methyl)-2H-indazol-2-yl)-2,2'-dimethyl-[l,r-biphenyl]-3-yl]-2Hindazol-6-yl)methyl)amino]ethan-l-ol; 2-[({2-[3'-(6-{[(2hy droxy ethyl)amino]methyl )imidazo[ 1,2-b]pyridazin-2-yl)-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 yl]imidazo[l,2-b]pyridazin-6-yl)methyl)amino]ethan-l-ol; 2-[({6-[3'-(2-{[(2hy droxy ethyl)amino]methyl )imidazo[ 1,2-b] [ 1,2,4]triazin-6-yl)-2,2'-dimethyl-[ 1,1 '-biphenyl]3-yl]imidazo[l,2-b][l,2,4]triazin-2-yl)methyl)amino]ethan-l-ol; 2-[({2-[3'-(6-{[(2hy droxy ethyl)amino]methyl )imidazo[ 1,2-a]pyrazin-2-yl)-2,2'-dimethyl-[ 1,1 '-biphenyl]-3 yl]imidazo[l,2-a]pyrazin-6-yl)methyl)amino]ethan-l-ol; 2-({[4-({3-[2-(4-{[(2hy droxy ethyl)amino]methyl)phenyl)-7-methyl-l,3-benzoxazol-6-yl]-2methylphenyl)methoxy)-3-methylphenyl]methyl)amino)ethan-l-ol; 2-({ [4-({3-[2-(3-{ [(2hy droxy ethyl)amino]methyl)phenyl)-7-methyl-l,3-benzoxazol-6-yl]-2methylphenyl)methoxy)-3-methylphenyl]methyl)amino)ethan-l-ol; 2-{[(4-{6-[2-(4-{[(2hy droxy ethyl)amino]methyl)phenyl)-7-methyl-l,3-benzoxazol-6-yl]-7-methyl-1,3benzoxazol-2-yl) phenyl)methyl] amino ) ethan- 1 -ol; or 2- {[(3 - { 6-[2-(3 - {[(2hy droxy ethyl)amino]methyl)phenyl)-7-methyl-l,3-benzoxazol-6-yl]-7-methyl-1,3benzoxazol-2-yl)phenyl)methyl]amino)ethan-l-ol, 2-[({2-[3'-(5-{[(2hy droxy ethyl)amino]methyl )-2,3-dihy dro-lH-isoindol-2-yl)-2,2'-dimethyl-[l,r-biphenyl]-3
    WO 2018/026971
    PCT/US2017/045185 yl]-2,3-dihydro-lH-isoindol-5-yl}methyl)amino]ethan-l-ol, or 2-[({6-[3'-(3-{[(2hydroxyethyl)amino]methyl}-2-methoxy-5H,6H,7H-pyrrolo[3,4-b]pyridin-6-yl)-2,2'dimethyl-[l,r-biphenyl]-3-yl]-2-methoxy-5H,6H,7H-pyrrolo[3,4-b]pyridin-3yl }methyl)amino]ethan-1 -ol
    12. A pharmaceutical composition comprising the compound of claim 1, 2, or 3, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
    13. A method of treating a disease associated with the modulation of PD-1/PD-L1 or CD80/PD-L1 interaction comprising administering to a patient the compound of claim 1, 2, or 3 or a pharmaceutically acceptable salt thereof.
    14. The method of claim 13, wherein the disease is an infection, inflammation, cancer, or neurodegenerative disorder.
    15. The method of claim 14, wherein the disease is Alzheimer’s disease.
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