AU2013263801B2 - Isolated Polypeptides, Polynucleotides Useful for Modifying Water User Efficiency, Fertilizer Use Efficiency, Biotic/Abiotic Stress Tolerance, Yield and Biomass in Plants - Google Patents

Abstract

Polynucleotides, polypeptides, plant cells expressing same and methods of using same for increasing abiotic stress tolerance water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant. The method is effected by expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80 % homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544 1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant.

Description

1 ISOLATED POLYPEPTIDES, POLYNUCLEOTIDES USEFUL FOR MODIFYING WATER USER EFFICIENCY, FERTILIZER USE EFFICIENCY, BIOTIC/ABIOTIC STRESS TOLERANCE, YIELD AND BIOMASS IN PLANTS 5 This application is a divisional of Australian Patent Application No. 2008344935 filed 23 December 2008, the entire contents of which is herein incorporated by cross-reference. The subject matter of this application is related to the applicant's International Patent Application No. PCT/IL2008/001657, filed 23 December 2008, as well as United States Provisional Patent Application No. 61/009,166 filed 27 December 2007 and No. 61/136,238 filed 20 August 2008, LO each of which is herein incorporated by cross-reference. FIELD AND BACKGROUND OF THE INVENTION The present invention, in some embodiments thereof, relates to novel aquaporin polynucleotides and polypeptides, and more particularly, but not exclusively, to methods of using same for increasing abiotic stress tolerance, water use efficiency (WUE), fertilizer use L5 efficiency (FUE), biomass, vigor and/or yield of a plant. Abiotic stress conditions such as salinity, drought, flood, suboptimal temperature and toxic chemical pollution, cause substantial damage to agricultural plants. Most plants have evolved strategies to protect themselves against these conditions. However, if the severity and duration of the stress conditions are too great, the effects on plant development, growth and !0 yield of most crop plants are profound. Furthermore, most of the crop plants are highly susceptible to abiotic stress (ABS) and thus necessitate optimal growth conditions for commercial crop yields. Continuous exposure to stress causes major alterations in the plant metabolism which ultimately leads to cell death and consequently yield losses. The global shortage of water supply is one of the most severe agricultural problems 25 affecting plant growth and crop yield and efforts are made to mitigate the harmful effects of desertification and salinization of the world's arable land. Thus, Agbiotech companies attempt to create new crop varieties which are tolerant to different abiotic stresses focusing mainly in developing new varieties that can tolerate water shortage for longer periods. Studies have shown that plant adaptations to adverse environmental conditions are 30 complex genetic traits with polygenic nature. When water supply is limited, the plant WUE is critical for the survival and yield of crop. Since water scarcity is increasing and water quality is reducing worldwide it is important to increase water productivity and plant WUE. Many of the environmental abiotic stresses, such as drought, low temperature or high salt, decrease root hydraulic conductance, affect plant growth and decrease crop productivity.

2 Genetic improvement of FUE in plants can be generated either via traditional breeding or via genetic engineering. Attempts to improve FUE in transgenic plants are described in U.S. Patent Applications 20020046419 to Choo, et al.; U.S. Pat. Appl. 20030233670 to Edgerton et al.; U.S. Pat. Appl. 20060179511 to Chomet et al.; Yanagisawa et al. [Proc. Natl. Acad. Sci. 5 U.S.A. 2004, 101(20):7833-8]; Good AG et al. [Trends Plant Sci. 2004, 9(12):597-605]; and U.S. Pat. No. 6,084,153 to Good et al. Aquaporins (AQPs), the water channel proteins, are involved in transport of water through the membranes, maintenance of cell water balance and homeostasis under changing environmental and developmental conditions [Maurel C. Plant aquaporins: Novel functions and LO regulation properties. FEBS Lett. 2007, 581(12):2227-36]. These proteins are considered to be the main passage enabling transport of water and small neutral solutes such as urea and CO 2 through the membrane [Maurel C. Plant aquaporins: Novel functions and regulation properties. FEBS Lett. 2007 Jun 12; 581(12):2227-36]. In plants, AQPs are present as four subfamilies of intrinsic proteins: plasma membrane (PIP), tonoplast (TIP), small and basic (SIP) and NOD26 L5 like (NIP). The total number of AQP members in plants, as compared to animals, appears to be surprisingly high [Maurel C., 2007 (Supra)]. For instance, 35 AQP genes have been identified in the Arabidopsis genome [Quigley F, et al., "From genome to function: the Arabidopsis aquaporins". Genome Biol. 2002, 3(1):RESEARCHOOO1.1-1.17], 36 in maize [Chaumont F, et al., 2001, "Aquaporins constitute a large and highly divergent protein family in maize. Plant !0 Physiol", 125(3):1206-15], and 33 in rice [Sakurai, J., et a., 2005, Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant Cell Physiol. 46, 1568 1577]. The high number of AQPs in plants suggests a diverse role and differential regulation under variable environmental conditions [Maurel C., 2007 (Supra)]. W02004/104162 to the present inventors teaches polynucleotide sequences and methods 25 of utilizing same for increasing the tolerance of a plant to abiotic stresses and/or increasing the biomass of a plant. W02007/020638 to the present inventors teaches polynucleotide sequences and methods of utilizing same for increasing the tolerance of a plant to abiotic stresses and/or increasing the biomass, vigor and/or yield of a plant. 30 Lian HL, et al., 2006 (Cell Res. 16: 651-60) over-expressed members of the PIP1 subgroup of AQPs in rice. Aharon R., et al. 2003 (Plant Cell, 15: 439-47) over-expressed the Arabidopsis plasma membrane aquaporin, PIPib, in transgenic tobacco plants.

3 SUMMARY OF THE INVENTION According to an aspect of some embodiments of the present invention there is provided a method of increasing abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at 5 least 80 % homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544 1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259, thereby increasing the abiotic stress tolerance of the plant. LO According to an aspect of some embodiments of the present invention there is provided a method of increasing water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80 % homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:33, L5 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555 1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant. According to an aspect of some embodiments of the present invention there is provided !0 an isolated polynucleotide comprising a nucleic acid sequence at least 80 % identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs:7, 8, 4, 1-3, 5, 6, 9 26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106 1111, 1113-1116, 1118-1134, 1136, 1138-1400,2748-2764,2843-2857 and 2859-3051. According to an aspect of some embodiments of the present invention there is provided a 25 nucleic acid construct, comprising the isolated polynucleotide of the invention and a promoter for directing transcription of the nucleic acid sequence. According to an aspect of some embodiments of the present invention there is provided an isolated polypeptide, comprising an amino acid sequence at least 80 % homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 30 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052 3065 and 3067-3259.

4 According to an aspect of some embodiments of the present invention there is provided a plant cell comprising an exogenous polypeptide having an amino acid sequence at least 80 % homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555 5 1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259. According to an aspect of some embodiments of the present invention there is provided a plant cell comprising an exogenous polynucleotide comprising a nucleic acid sequence at least 80 % homologous to the nucleic acid sequence selected from the group consisting of SEQ ID LO NOs:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482 519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857 and 2859-3051. According to an aspect of some embodiments of the present invention there is provided a method of increasing abiotic stress tolerance of a plant, comprising expressing within the L5 plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence set forth by SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259, thereby increasing the abiotic stress tolerance of the plant. !0 According to an aspect of some embodiments of the present invention there is provided a method of increasing water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid sequence set forth by SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544 25 1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant. According to an aspect of some embodiments of the present invention there is provided 30 an isolated polynucleotide comprising the nucleic acid sequence set forth by SEQ ID NO:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521 1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857, 2859-3050 or 3051.

5 According to an aspect of some embodiments of the present invention there is provided a nucleic acid construct, comprising the isolated polynucleotide of the invention and a promoter for directing transcription of the nucleic acid sequence. According to an aspect of some embodiments of the present invention there is provided 5 an isolated polypeptide, comprising the amino acid sequence set forth by SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555 1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485 2746, 2765-2769, 3052-3065, 3067-3258 or 3259. According to an aspect of some embodiments of the present invention there is provided LO a plant cell comprising an exogenous polypeptide having the amino acid sequence set forth by SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259. According to an aspect of some embodiments of the present invention there is provided L5 a plant cell comprising an exogenous polynucleotide comprising the nucleic acid sequence set forth by SEQ ID NO:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857, 2859-3050 or 3051. According to some embodiments of the invention, the polynucleotide is selected from !0 the group consisting of SEQ ID NOs:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857 and 2859-3051. According to some embodiments of the invention, the amino acid sequence is selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405 25 1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453 2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259. According to some embodiments of the invention, the polypeptide is selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437 1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460 30 2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259. According to some embodiments of the invention, the abiotic stress is selected from the group consisting of salinity, water deprivation, low temperature, high temperature, heavy metal 6 toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation. According to some embodiments of the invention, the method further comprising growing the plant expressing the exogenous polynucleotide under the abiotic stress. According to some embodiments of the invention, the promoter is a constitutive promoter. According to some embodiments of the invention, the plant cell forms a part of a plant. Unless otherwise defined, all technical and/or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of embodiments of the invention, exemplary methods and/or materials are described below. In case of conflict, the patent specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be necessarily limiting. Definitions of the specific embodiments of the invention as claimed herein follow. According to a first embodiment of the invention, there is provided a method of increasing abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80 % homologous to the amino acid sequence set forth in SEQ ID NO: 32 or 2766, thereby increasing the abiotic stress tolerance of the plant. According to a second embodiment of the invention, there is provided a method of increasing water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80 % homologous to the amino acid sequence set forth in SEQ ID NO: 32 or 2766, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant. According to a third embodiment of the invention, there is provided a method of increasing abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence exhibiting at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766, thereby increasing the abiotic stress tolerance of the plant. According to a fourth embodiment of the invention, there is provided a method of increasing water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a 7 polypeptide comprising an amino acid sequence exhibiting at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant. According to a fifth embodiment of the invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence at least 80 % identical to the nucleic acid sequence set forth in SEQ ID NO: 6 or 2755. According to a sixth embodiment of the invention, there is provided an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence exhibiting at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766. According to a seventh embodiment of the invention, there is provided a nucleic acid construct comprising the isolated polynucleotide of the fifth or sixth embodiment and a promoter for directing transcription of said nucleic acid sequence in a host cell. According to an eighth embodiment of the invention, there is provided an isolated polypeptide comprising an amino acid sequence exhibiting at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766. According to a ninth embodiment of the invention, there is provided a plant cell comprising an exogenous polypeptide having an amino acid sequence exhibiting at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766. According to a tenth embodiment of the invention, there is provided a plant cell comprising the nucleic acid construct of the seventh embodiment. According to an eleventh embodiment of the invention, there is provided a transgenic plant having increased abiotic stress tolerance as compared to a control plant, comprising the nucleic acid construct of the seventh embodiment. BRIEF DESCRIPTION OF THE DRAWINGS Some embodiments of the invention are herein described, by way of example only, with reference to the accompanying drawings. With specific reference now to the drawings in detail, it is stressed that the particulars shown are by way of example and for purposes of illustrative discussion of embodiments of the invention. In this regard, the description taken with the drawings makes apparent to those skilled in the art how embodiments of the invention may be practiced.

7a In the drawings: FIG. 1 is a schematic illustration of the pGI binary plasmid used for expressing the isolated polynucleotide sequences of the invention. RB - T-DNA right border; LB - T-DNA left border; H- HindIll restriction enzyme; X - XbaI restriction enzyme; B - BamHI restriction enzyme; S - SalI restriction enzyme; Sm - SmaI restriction enzyme; R-I - EcoRI restriction enzyme; Sc - SacI/SstI/Ecl136II; (numbers) - Length in base-pairs; NOS pro nopaline synthase promoter; NPT-II = neomycin phosphotransferase gene; NOS ter = nopaline synthase terminator; Poly-A signal (polyadenylation signal); GUSintron - the GUS reporter gene (coding sequence and intron). The isolated polynucleotide sequences of some embodiments of the invention were cloned into the vector while replacing the GUSintron reporter gene. [Text continues on page 8.1 8 FIGs. 2A-B are images depicting root development of plants grown in transparent agar plates. The different transgenes were grown in transparent agar plates for 10-15 days and the plates were photographed every 2-5 days starting at day 1. FIG. 2A - An exemplary image of plants taken following 12 days on agar plates. FIG. 2B - An exemplary image of root analysis 5 in which the length of the root measured is represented by a red arrow. FIGs. 3A-F are histograms depicting the total economic fruit yield, plant biomass and harvest index for TOM-ABST36 (black bar) vs. control (white bar) plants growing in the commercial greenhouse under a 200 mM sodium chloride (NaCl) irrigation regime (FIG. 3A-C, respectively), or under two different water-stress regimes (WLI-1 and WLI-2; FIG. 3D-F, LO respectively). Yield performance was compared to plants growing under standard irrigation conditions (0 mM NaCl and WLI-0). Results are the average of the four independent events. *Significantly different at P < 0.05. FIGs. 3G-J are photographs of transgenic tomato plants or control plants grown under various conditions. FIG. 3G - TOM-ABST36 plants growing under regular irrigation L5 conditions; FIG. 3H - control plants growing under regular irrigation conditions; FIG. 31 TOM-ABST36 plants after growing under a 200-mM NaCl-irrigation regime during the entire growing season; FIG. 3J - control plants after growing under a 200-mM NaCl-irrigation regime during the entire growing season. !0 DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION The present invention, in some embodiments thereof, relates to novel aquaporin polynucleotides and polypeptides, and more particularly, but not exclusively, to methods of using same for increasing abiotic stress tolerance, water use efficiency, fertilizer use efficiency, biomass, vigor and/or yield of a plant. 25 Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not necessarily limited in its application to the details set forth in the following description or exemplified by the Examples. The invention is capable of other embodiments or of being practiced or carried out in various ways. While reducing the invention to practice, the present inventors have identified novel 30 aquaporin (AQP) polynucleotides and polypeptides encoded thereby. Thus, as shown in the Examples section which follows, the present inventors have employed a bioinformatics approach which combines digital expression analysis and cross species comparative genomics and screened 7.2 million expressed sequence tags (ESTs) from 9 1,195 relevant EST's libraries of both monocot and dicot plant species. Using this approach 1,114 different AQP genes have been identified and were further classified to 11 subgroups (Table 1). Further analysis revealed that ESTs of the TIP2 subgroup are significantly over represented in both plants' roots and in plants exposed to abiotic stress (ABS), and that 5 polypeptides (e.g., SEQ ID NOs: 27-28, 45-48, Table 2) encoded by polynucleotides of the TIP2 subgroup (e.g., SEQ ID NOs:1, 2, 19-22, Table 2) share a common consensus sequence TLXFXFAGVGS (SEQ ID NO:2826). Based on over-representation in roots, ABS conditions and tissues with low water levels (such as seed and pollen) additional polynucleotides of the aquaporin gene family were identified (SEQ ID NOs: 3-18, 23-26, Table 2), as well as LO homologues or orthologues thereof (SEQ ID NOs:53-1400, 2844-3051 for polynucleotides and SEQ ID NOs:1401-2746, 3052-3259 for polypeptides; Table 3). Moreover, quantitative RT PCR analysis demonstrated increased expression of representative AQP genes (e.g., SEQ ID NOs:5, 6 and 7) under salt stress, which was higher in plants exhibiting salt tolerance as compared to plants which are sensitive to salt stress (Table 5, Example 2 of the Examples L5 section which follows). As is further described in Examples 3-4 of the Examples section which follows, representative AQP polynucleotides were cloned (Tables 7, 8 and 9) and transgenic plants over-expressing same were generated (Example 4). These plants were shown to exhibit increased tolerance to various abiotic stresses such as osmotic stress (Tables 10-14; Example 5) and salinity stress (Tables 30-45; Example 6), increased fertilizer use efficiency (under nitrogen !0 limiting conditions, Tables 59-68, Example 7) and increased growth, biomass and yield under normal [Tables 15-29 (Example 5), 46-58 (Example 6)] or abiotic stress conditions conditions (Examples 5-8). Altogether, these results suggest the use of the AQP polynucleotides and polypeptides of the invention for increasing abiotic stress tolerance, water use efficiency, fertilizer use efficiency, biomass, vigor and/or yield of a plant. 25 It should be noted that polypeptides or polynucleotides which affect (e.g., increase) plant metabolism, growth, reproduction and/or viability under stress, can also affect the plant growth, biomass, yield and/or vigor under optimal conditions. Thus, according to one aspect of the invention, there is provided a method of increasing abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield 30 and/or vigor of a plant. The method is effected by expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising the amino acid consensus sequence TLXFXFAGVGS as set forth by SEQ ID NO:2826, wherein expression of the polypeptide promotes plants' biomass/ vigor and/or yield under normal or stress conditions.

10 It is suggested that the polypeptide's activity is structurally associated with the integrity of the above consensus sequence (SEQ ID NO:2826). In some embodiments of this aspect of the present invention, the activity is a water channel activity which typically resides in the vacuaolar membrane (tonoplast) and/or the plasma membrane of the plant cell and enables the 5 transport of water and/or small neutral solutes such as urea, nitrates and carbon dioxide (C0 2 ) through the membrane. The phrase "abiotic stress" as used herein refers to any adverse effect on metabolism, growth, reproduction and/or viability of a plant. Accordingly, abiotic stress can be induced by suboptimal environmental growth conditions such as, for example, salinity, water deprivation, LO flooding, freezing, low or high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, atmospheric pollution or UV irradiation. The implications of abiotic stress are discussed in the Background section. The phrase "abiotic stress tolerance" as used herein refers to the ability of a plant to endure an abiotic stress without suffering a substantial alteration in metabolism, growth, L5 productivity and/or viability. As used herein the phrase "water use efficiency (WUE)" refers to the level of organic matter produced per unit of water consumed by the plant, i.e., the dry weight of a plant in relation to the plant's water use, e.g., the biomass produced per unit transpiration. As used herein the phrase "fertilizer use efficiency" refers to the uptake, spread, !0 absorbent, accumulation, relocation (within the plant) and use of one or more of the minerals and organic moieties absorbed from the soil, such as nitrogen, phosphates and/or potassium. As used herein the phrase "plant biomass" refers to the amount (measured in grams of air-dry tissue) of a tissue produced from the plant in a growing season, which could also determine or affect the plant yield or the yield per growing area. 25 As used herein the phrase "plant yield" refers to the amount (as determined by weight/size) or quantity (numbers) of tissue produced per plant or per growing season. Hence increased yield could affect the economic benefit one can obtain from the plant in a certain growing area and/or growing time. As used herein the phrase "plant vigor" refers to the amount (measured by weight) of 30 tissue produced by the plant in a given time. Hence increase vigor could determine or affect the plant yield or the yield per growing time or growing area. As used herein the term "increasing" refers to at least about 2 %, at least about 3 %, at least about 4 %, at least about 5 %, at least about 10 %, at least about 15 %, at least about 20 %, 11 at least about 30 %, at least about 40 %, at least about 50 %, at least about 60 %, at least about 70 %, at least about 80 %, increase in plant abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor as compared to a native plant [i.e., a plant not modified with the biomolecules (polynucleotide or polypeptides) of the invention, 5 e.g., a non-transformed plant of the same species which is grown under the same growth conditions). As used herein, the phrase "exogenous polynucleotide" refers to a heterologous nucleic acid sequence which may not be naturally expressed within the plant or which overexpression in the plant is desired. The exogenous polynucleotide may be introduced into the plant in a LO stable or transient manner, so as to produce a ribonucleic acid (RNA) molecule and/or a polypeptide molecule. It should be noted that the exogenous polynucleotide may comprise a nucleic acid sequence which is identical or partially homologous to an endogenous nucleic acid sequence of the plant. According to some embodiments of the invention, the exogenous polynucleotide of the L5 invention encodes a polypeptide having an amino acid sequence at least about 60 %, at least about 65 %, at least about 70 %, at least about 75 %, at least about 80 %, at least about 81 %, at least about 82 %, at least about 83 %, at least about 84 %, at least about 85 %, at least about 86 %, at least about 87 %, at least about 88 %, at least about 89 %, at least about 90 %, at least about 91 %, at least about 92 %, at least about 93 %, at least about 94 %, at least about 95 %, at !0 least about 96 %, at least about 97 %, at least about 98 %, at least about 99 %, or more say 100 % homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 27-28, 45-48, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561, 2449-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2484 and 2765. Homology (e.g., percent homology) can be determined using any homology comparison 25 software, including for example, the BlastP or TBLASTN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters, when starting from a polypeptide sequence; or the tBLASTX algorithm (available via the NCBI) such as by using default parameters, which compares the six-frame conceptual translation products of a nucleotide query sequence (both strands) against a protein sequence database. 30 Homologous sequences include both orthologous and paralogous sequences. The term "paralogous" relates to gene-duplications within the genome of a species leading to paralogous genes. The term "orthologous" relates to homologous genes in different organisms due to ancestral relationship.

12 One option to identify orthologues in monocot plant species is by performing a reciprocal blast search. This may be done by a first blast involving blasting the sequence-of interest against any sequence database, such as the publicly available NCBI database which may be found at: Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih 5 (dot) gov. If orthologues in rice were sought, the sequence-of-interest would be blasted against, for example, the 28,469 full-length cDNA clones from Oryza sativa Nipponbare available at NCBI. The blast results may be filtered. The full-length sequences of either the filtered results or the non-filtered results are then blasted back (second blast) against the sequences of the organism from which the sequence-of-interest is derived. The results of the first and second LO blasts are then compared. An orthologue is identified when the sequence resulting in the highest score (best hit) in the first blast identifies in the second blast the query sequence (the original sequence-of-interest) as the best hit. Using the same rational a paralogue (homolog to a gene in the same organism) is found. In case of large sequence families, the ClustalW program may be used [Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) L5 uk/Tools/clustalw2/index (dot) html], followed by a neighbor-joining tree (Hypertext Transfer Protocol://en (dot) wikipedia (dot) org/wiki/Neighbor-joining) which helps visualizing the clustering. According to some embodiments of the invention, the exogenous polynucleotide encodes a polypeptide consisting of the amino acid sequence set forth by SEQ ID NO:27-28, !0 45-48, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555-1559, 1561, 2449 2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2484 or 2765. According to some embodiments of the invention the exogenous polynucleotide comprises a nucleic acid sequence which is at least about 60 %, at least about 65 %, at least about 70 %, at least about 75 %, at least about 80 %, at least about 81 %, at least about 82 %, at 25 least about 83 %, at least about 84 %, at least about 85 %, at least about 86 %, at least about 87 %, at least about 88 %, at least about 89 %, at least about 90 %, at least about 91 %, at least about 92 %, at least about 93 %, at least about 93 %, at least about 94 %, at least about 95 %, at least about 96 %, at least about 97 %, at least about 98 %, at least about 99 %, e.g., 100 % identical to the nucleic acid sequence selected from the group consisting of SEQ ID NOs:1, 2, 30 19, 20-22, 53-55, 57-87, 89-141, 143-147, 149-195, 197-206, 208-212, 214, 1102-1103, 1106 1111, 1113-1116, 1118-1134, 1136, 2751-2752 and 2748-2750.

13 Identity (e.g., percent homology) can be determined using any homology comparison software, including for example, the BlastN software of the National Center of Biotechnology Information (NCBI) such as by using default parameters. According to some embodiments of the invention the exogenous polynucleotide is set 5 forth by SEQ ID NO:1, 2, 19, 20-22, 53-55, 57-87, 89-141, 143-147, 149-195, 197-206, 208 212, 214, 1102-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 2751-2752, 2748-2749, or 2750. Notwithstanding the above, additional AQP polynucleotides and polypeptides encoded thereby are contemplated by the present teachings. LO According to some embodiments of the invention, the exogenous polynucleotide encodes a polypeptide having an amino acid sequence at least about 60 %, at least about 65 %, at least about 70 %, at least about 75 %, at least about 80 %, at least about 85 %, at least about 86 %, at least about 87 %, at least about 88 %, at least about 89 %, at least about 90 %, at least about 91 %, at least about 92 %, at least about 93 %, at least about 94 %, at least about 95 %, at L5 least about 96 %, at least about 97 %, at least about 98 %, at least about 99 %, e.g., 100 % homologous to SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437 1494, 1496-1542, 1544-1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259. According to some embodiments of the invention, the exogenous polynucleotide !0 encodes a polypeptide consisting of the amino acid sequence set forth by SEQ ID NO:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544-1553, 1555 1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485 2746, 2765-2769, 3052-3065, 3067-3258 or 3259. In an exemplary embodiment the exogenous polynucleotide does not encode a 25 polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NOs: 1828, 1867, 1404, 1436, 1495, 1543, 1554, 1560, 2451, 2452, 2459, 2464, 2482, 2484 and 3066. According to some embodiments of the invention, the exogenous polynucleotide is at least at least about 60 %, least at least about 65 %, least at least about 70 %, least at least about 30 75 %least at least about 80 %, at least about 85 %, at least about 86 %, at least about 87 %, at least about 88 %, at least about 89 %, at least about 90 %, at least about 91 %, at least about 92 %, at least about 93 %, at least about 94 %, at least about 95 %, at least about 96 %, at least about 97 %, at least about 98 %, at least about 99 %, e.g., 100 % identical to SEQ ID NO:7, 8, 14 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521 1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843-2857, 2859-3050 or 3051. According to some embodiments of the invention, the polynucleotide is set forth by 5 SEQ ID NO:7, 8, 4, 1-3, 5, 6, 9-26, 53-55, 57-87, 89-147, 149-195, 197-206, 208-212, 214-480, 482-519, 521-1103, 1106-1111, 1113-1116, 1118-1134, 1136, 1138-1400, 2748-2764, 2843 2857, 2859-3050 or 3051. In an exemplary embodiment the exogenous polynucleotide is not the polynucleotide set forth by SEQ ID NO: 481, 520, 56, 88, 148, 196, 207, 213, 1104, 1105, 1112, 1117, 1135, 1137 LO or 2858. As used herein the term "polynucleotide" refers to a single or double stranded nucleic acid sequence which is isolated and provided in the form of an RNA sequence, a complementary polynucleotide sequence (cDNA), a genomic polynucleotide sequence and/or a composite polynucleotide sequences (e.g., a combination of the above). L5 As used herein the phrase "complementary polynucleotide sequence" refers to a sequence, which results from reverse transcription of messenger RNA using a reverse transcriptase or any other RNA dependent DNA polymerase. Such a sequence can be subsequently amplified in vivo or in vitro using a DNA dependent DNA polymerase. As used herein the phrase "genomic polynucleotide sequence" refers to a sequence !0 derived (isolated) from a chromosome and thus it represents a contiguous portion of a chromosome. As used herein the phrase "composite polynucleotide sequence" refers to a sequence, which is at least partially complementary and at least partially genomic. A composite sequence can include some exonal sequences required to encode the polypeptide of the present invention, 25 as well as some intronic sequences interposing therebetween. The intronic sequences can be of any source, including of other genes, and typically will include conserved splicing signal sequences. Such intronic sequences may further include cis acting expression regulatory elements. According to some embodiments of the invention, the polynucleotide of the invention 30 comprises no more than 5000 nucleic acids in length. According to some embodiments of the invention, the polynucleotide of the invention comprises no more than 4000 nucleic acids in length, e.g., no more than 3000 nucleic acids, e.g., no more than 2500 nucleic acids.

15 Nucleic acid sequences encoding the polypeptides of the present invention may be optimized for expression. A non-limiting example of an optimized nucleic acid sequence is provided in SEQ ID NO:2751, which encodes an optimized polypeptide comprising the amino acid sequence set forth by SEQ ID NO:27. Examples of such sequence modifications include, 5 but are not limited to, an altered G/C content to more closely approach that typically found in the plant species of interest, and the removal of codons atypically found in the plant species commonly referred to as codon optimization. The phrase "codon optimization" refers to the selection of appropriate DNA nucleotides for use within a structural gene or fragment thereof that approaches codon usage within the LO plant of interest. Therefore, an optimized gene or nucleic acid sequence refers to a gene in which the nucleotide sequence of a native or naturally occurring gene has been modified in order to utilize statistically-preferred or statistically-favored codons within the plant. The nucleotide sequence typically is examined at the DNA level and the coding region optimized for expression in the plant species determined using any suitable procedure, for example as L5 described in Sardana et al. (1996, Plant Cell Reports 15:677-681). In this method, the standard deviation of codon usage, a measure of codon usage bias, may be calculated by first finding the squared proportional deviation of usage of each codon of the native gene relative to that of highly expressed plant genes, followed by a calculation of the average squared deviation. The formula used is: 1 SDCU = n = 1 N [ ( Xn - Yn ) / Yn ] 2 / N, where Xn refers to the frequency !0 of usage of codon n in highly expressed plant genes, where Yn to the frequency of usage of codon n in the gene of interest and N refers to the total number of codons in the gene of interest. A Table of codon usage from highly expressed genes of dicotyledonous plants is compiled using the data of Murray et al. (1989, Nuc Acids Res. 17:477-498). One method of optimizing the nucleic acid sequence in accordance with the preferred 25 codon usage for a particular plant cell type is based on the direct use, without performing any extra statistical calculations, of codon optimization Tables such as those provided on-line at the Codon Usage Database through the NIAS (National Institute of Agrobiological Sciences) DNA bank in Japan (Hypertext Transfer Protocol://World Wide Web (dot) kazusa (dot) or (dot) jp/codon/). The Codon Usage Database contains codon usage tables for a number of different 30 species, with each codon usage Table having been statistically determined based on the data present in Genbank. By using the above Tables to determine the most preferred or most favored codons for each amino acid in a particular species (for example, rice), a naturally-occurring nucleotide 16 sequence encoding a protein of interest can be codon optimized for that particular plant species. This is effected by replacing codons that may have a low statistical incidence in the particular species genome with corresponding codons, in regard to an amino acid, that are statistically more favored. However, one or more less-favored codons may be selected to delete existing 5 restriction sites, to create new ones at potentially useful junctions (5' and 3' ends to add signal peptide or termination cassettes, internal sites that might be used to cut and splice segments together to produce a correct full-length sequence), or to eliminate nucleotide sequences that may negatively effect mRNA stability or expression. The naturally-occurring encoding nucleotide sequence may already, in advance of any LO modification, contain a number of codons that correspond to a statistically-favored codon in a particular plant species. Therefore, codon optimization of the native nucleotide sequence may comprise determining which codons, within the native nucleotide sequence, are not statistically favored with regards to a particular plant, and modifying these codons in accordance with a codon usage table of the particular plant to produce a codon optimized derivative. A modified L5 nucleotide sequence may be fully or partially optimized for plant codon usage provided that the protein encoded by the modified nucleotide sequence is produced at a level higher than the protein encoded by the corresponding naturally occurring or native gene. Construction of synthetic genes by altering the codon usage is described in for example PCT Patent Application 93/07278. !0 Thus, the invention encompasses nucleic acid sequences described hereinabove; fragments thereof, sequences hybridizable therewith, sequences homologous thereto, sequences encoding similar polypeptides with different codon usage, altered sequences characterized by mutations, such as deletion, insertion or substitution of one or more nucleotides, either naturally occurring or man induced, either randomly or in a targeted fashion. 25 As mentioned, the present inventors have uncovered previously uncharacterized polypeptides which share the amino acid consensus sequence set forth by SEQ ID NO:2826. Thus, the invention provides an isolated polypeptide having an amino acid sequence at least about 60 %, at least about 65 %, at least about 70 %, at least about 75 %, at least about 80 %, at least about 81 %, at least about 82 %, at least about 83 %, at least about 84 %, at least 30 about 85 %, at least about 86 %, at least about 87 %, at least about 88 %, at least about 89 %, at least about 90 %, at least about 91 %, at least about 92 %, at least about 93 %, at least about 93 %, at least about 94 %, at least about 95 %, at least about 96 %, at least about 97 %, at least about 98 %, at least about 99 %, or more say 100 % homologous to an amino acid sequence 17 selected from the group consisting of SEQ ID NO: 27-28, 45-48, 1401-1403, 1405-1435, 1437 1494, 1496-1542, 1544-1553, 1555-1559, 1561, 2449-2450, 2453-2458, 2460-2463, 2465 2481, 2483, 2484 and 2765. According to some embodiments of the invention, the invention provides an isolated 5 polypeptide having an amino acid sequence at least about 60 %, at least about 65 %, at least about 70 %, at least about 75 %, at least about 80 %, at least about 81 %, at least about 82 %, at least about 83 %, at least about 84 %, at least about 85 %, at least about 86 %, at least about 87 %, at least about 88 %, at least about 89 %, at least about 90 %, at least about 91 %, at least about 92 %, at least about 93 %, at least about 93 %, at least about 94 %, at least about 95 %, at LO least about 96 %, at least about 97 %, at least about 98 %, at least about 99 %, or more say 100 % homologous to an amino acid sequence selected from the group consisting of SEQ ID NOs:33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544 1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065 and 3067-3259. L5 According to some embodiments of the invention, the polypeptide is set forth by SEQ ID NO: 33, 34, 30, 27-29, 31, 32, 35-52, 1401-1403, 1405-1435, 1437-1494, 1496-1542, 1544 1553, 1555-1559, 1561-1827, 1829-1866, 1868-2450, 2453-2458, 2460-2463, 2465-2481, 2483, 2485-2746, 2765-2769, 3052-3065, 3067-3258 or 3259. In an exemplary embodiment the polypeptide is not the polypeptide set forth by SEQ ID !0 NO: 1828, 1867, 1404, 1436, 1495, 1543, 1554, 1560, 2451, 2452, 2459, 2464, 2482, 2484 or 3066. The invention also encompasses fragments of the above described polypeptides and polypeptides having mutations, such as deletions, insertions or substitutions of one or more amino acids, either naturally occurring or man induced, either randomly or in a targeted 25 fashion. The term "plant" as used herein encompasses whole plants, ancestors and progeny of the plants and plant parts, including seeds, shoots, stems, roots (including tubers), and plant cells, tissues and organs. The plant may be in any form including suspension cultures, embryos, meristematic regions, callus tissue, leaves, gametophytes, sporophytes, pollen, and microspores. 30 Plants that are particularly useful in the methods of the invention include all plants which belong to the superfamily Viridiplantae, in particular monocotyledonous and dicotyledonous plants including a fodder or forage legume, ornamental plant, food crop, tree, or shrub selected from the list comprising Acacia spp., Acer spp., Actinidia spp., Aesculus spp., Agathis 18 australis, Albizia amara, Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Astelia fragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassica spp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadaba farinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicum spp., Cassia spp., Centroema pubescens, Chacoomeles spp., 5 Cinnamomum cassia, Coffea arabica, Colophospermum mopane, Coronillia varia, Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp., Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogon spp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davallia divaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogon amplectens, Dioclea spp, Dolichos spp., Dorycnium rectum, Echinochloa pyramidalis, Ehraffia LO spp., Eleusine coracana, Eragrestis spp., Erythrina spp., Eucalypfus spp., Euclea schimperi, Eulalia vi/losa, Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp, Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycine javanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtia coleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus, Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffhelia L5 dissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia, Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex, Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihot esculenta, Medicago saliva, Metasequoia glyptostroboides, Musa sapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryza spp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petunia spp., Phaseolus spp., Phoenix !0 canariensis, Phormium cookianum, Photinia spp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara, Pogonarthria fleckii, Pogonaffhria squarrosa, Populus spp., Prosopis cineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis, Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhus natalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosa spp., Rubus spp., Salix spp., Schyzachyrium sanguineum, 25 Sciadopitys vefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghum bicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides, Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themeda triandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vaccinium spp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschia aethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brussels sprouts, cabbage, canola, carrot, 30 cauliflower, celery, collard greens, flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean, straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize, wheat, barely, rye, oat, peanut, pea, lentil and alfalfa, cotton, rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, a tree, an ornamental plant, a perennial grass and a forage crop.

19 Alternatively algae and other non-Viridiplantae can be used for the methods of the present invention. According to some embodiments of the invention, the plant used by the method of the invention is a crop plant such as rice, maize, wheat, barley, peanut, potato, sesame, olive tree, 5 palm oil, banana, soybean, sunflower, canola, sugarcane, alfalfa, millet, leguminosae (bean, pea), flax, lupinus, rapeseed, tobacco, poplar and cotton. Expressing the exogenous polynucleotide of the invention within the plant can be effected by transforming one or more cells of the plant with the exogenous polynucleotide, followed by generating a mature plant from the transformed cells and cultivating the mature LO plant under conditions suitable for expressing the exogenous polynucleotide within the mature plant. According to some embodiments of the invention, the transformation is effected by introducing to the plant cell a nucleic acid construct which includes the exogenous polynucleotide of some embodiments of the invention and at least one promoter capable of L5 directing transcription of the exogenous polynucleotide in the plant cell. Further details of suitable transformation approaches are provided hereinbelow. As used herein, the term "promoter" refers to a region of DNA which lies upstream of the transcriptional initiation site of a gene to which RNA polymerase binds to initiate transcription of RNA. The promoter controls where (e.g., which portion of a plant) and/or !0 when (e.g., at which stage or condition in the lifetime of an organism) the gene is expressed. Any suitable promoter sequence can be used by the nucleic acid construct of the present invention. Preferably the promoter is a constitutive promoter, a tissue-specific, or an abiotic stress-inducible promoter. Suitable constitutive promoters include, for example, CaMV 35S promoter (SEQ ID 25 NO:2825; Odell et al., Nature 313:810-812, 1985); Arabidopsis At6669 promoter (SEQ ID NO:2823; see PCT Publication No. W004081173A2); maize Ubi 1 (Christensen et al., Plant Sol. Biol. 18:675-689, 1992); rice actin (McElroy et al., Plant Cell 2:163-171, 1990); pEMU (Last et al., Theor. Appl. Genet. 81:581-588, 1991); CaMV 19S (Nilsson et al., Physiol. Plant 100:456-462, 1997); GOS2 (de Pater et al, Plant J Nov;2(6):837-44, 1992); ubiquitin 30 (Christensen et al, Plant Mol. Biol. 18: 675-689, 1992); Rice cyclophilin (Bucholz et al, Plant Mol Biol. 25(5):837-43, 1994); Maize H3 histone (Lepetit et al, Mol. Gen. Genet. 231: 276 285, 1992); Actin 2 (An et al, Plant J. 10(1);107-121, 1996) and Synthetic Super MAS (Ni et al., The Plant Journal 7: 661-76, 1995). Other constitutive promoters include those in U.S. Pat.

20 Nos. 5,659,026, 5,608,149; 5.608,144; 5,604,121; 5.569,597: 5.466,785; 5,399,680; 5,268,463; and 5,608,142. Suitable tissue-specific promoters include, but not limited to, leaf-specific promoters [such as described, for example, by Yamamoto et al., Plant J. 12:255-265, 1997; Kwon et al., 5 Plant Physiol. 105:357-67, 1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor et al., Plant J. 3:509-18, 1993; Orozco et al., Plant Mol. Biol. 23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA 90:9586-9590, 1993], seed-preferred promoters [e.g., from seed specific genes (Simon, et al., Plant Mol. Biol. 5. 191, 1985; Scofield, et al., J. Biol. Chem. 262: 12202, 1987; Baszczynski, et al., Plant Mol. Biol. 14: 633, 1990), Brazil Nut LO albumin (Pearson' et al., Plant Mol. Biol. 18: 235- 245, 1992), legumin (Ellis, et al. Plant Mol. Biol. 10: 203-214, 1988), Glutelin (rice) (Takaiwa, et al., Mol. Gen. Genet. 208: 15-22, 1986; Takaiwa, et al., FEBS Letts. 221: 43-47, 1987), Zein (Matzke et al Plant Mol Biol, 143).323-32 1990), napA (Stalberg, et al, Planta 199: 515-519, 1996), Wheat SPA (Albanietal, Plant Cell, 9: 171- 184, 1997), sunflower oleosin (Cummins, etal., Plant Mol. Biol. 19: 873- 876, 1992)], L5 endosperm specific promoters [e.g., wheat LMW and HMW, glutenin-1 (Mol Gen Genet 216:81-90, 1989; NAR 17:461-2), wheat a, b and g gliadins (EMBO3:1409-15, 1984), Barley ltrl promoter, barley B1, C, D hordein (Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; Mol Gen Genet 250:750- 60, 1996), Barley DOF (Mena et al, The Plant Journal, 116(1): 53- 62, 1998), Biz2 (EP99106056.7), Synthetic promoter (Vicente-Carbajosa et al., Plant J. 13: !0 629-640, 1998), rice prolamin NRP33, rice -globulin Glb-1 (Wu et al, Plant Cell Physiology 39(8) 885- 889, 1998), rice alpha-globulin REB/OHP-1 (Nakase et al. Plant Mol. Biol. 33: 513 S22, 1997), rice ADP-glucose PP (Trans Res 6:157-68, 1997), maize ESR gene family (Plant J 12:235-46, 1997), sorgum gamma- kafirin (PMB 32:1029-35, 1996)], embryo specific promoters [e.g., rice OSH1 (Sato et al, Proc. Nati. Acad. Sci. USA, 93: 8117-8122), KNOX 25 (Postma-Haarsma ef al, Plant Mol. Biol. 39:257-71, 1999), rice oleosin (Wu et at, J. Biochem., 123:386, 1998)], and flower-specific promoters [e.g., AtPRP4, chalene synthase (chsA) (Van der Meer, et al., Plant Mol. Biol. 15, 95-109, 1990), LAT52 (Twell et al Mol. Gen Genet. 217:240-245; 1989), apetala- 3]. Suitable abiotic stress-inducible promoters include, but not limited to, salt-inducible 30 promoters such as RD29A (Yamaguchi-Shinozalei et al., Mol. Gen. Genet. 236:331-340, 1993); drought-inducible promoters such as maize rabl7 gene promoter (Pla et. al., Plant Mol. Biol. 21:259-266, 1993), maize rab28 gene promoter (Busk et. al., Plant J. 11:1285-1295, 1997) and 21 maize Ivr2 gene promoter (Pelleschi et. al., Plant Mol. Biol. 39:373-380, 1999); heat-inducible promoters such as heat tomato hsp8O-promoter from tomato (U.S. Pat. No. 5,187,267). The nucleic acid construct of some embodiments of the invention can further include an appropriate selectable marker and/or an origin of replication. According to some embodiments 5 of the invention, the nucleic acid construct utilized is a shuttle vector, which can propagate both in E. coli (wherein the construct comprises an appropriate selectable marker and origin of replication) and be compatible with propagation in cells. The construct according to the present invention can be, for example, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus or an artificial chromosome. LO The nucleic acid construct of some embodiments of the invention can be utilized to stably or transiently transform plant cells. In stable transformation, the exogenous polynucleotide is integrated into the plant genome and as such it represents a stable and inherited trait. In transient transformation, the exogenous polynucleotide is expressed by the cell transformed but it is not integrated into the genome and as such it represents a transient L5 trait. There are various methods of introducing foreign genes into both monocotyledonous and dicotyledonous plants (Potrykus, I., Annu. Rev. Plant. Physiol., Plant. Mol. Biol. (1991) 42:205-225; Shimamoto et al., Nature (1989) 338:274-276). The principle methods of causing stable integration of exogenous DNA into plant !0 genomic DNA include two main approaches: (i) Agrobacterium-mediated gene transfer: Klee et al. (1987) Annu. Rev. Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes, eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds. Kung, S. 25 and Arntzen, C. J., Butterworth Publishers, Boston, Mass. (1989) p. 93-112. (ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego, Calif. (1989) p. 52-68; including methods for direct uptake of DNA into protoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNA 30 uptake induced by brief electric shock of plant cells: Zhang et al. Plant Cell Rep. (1988) 7:379 384. Fromm et al. Nature (1986) 319:791-793. DNA injection into plant cells or tissues by particle bombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al. Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990) 79:206-209; by the use of 22 micropipette systems: Neuhaus et al., Theor. Apple. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant. (1990) 79:213-217; glass fibers or silicon carbide whisker transformation of cell cultures, embryos or callus tissue, U.S. Pat. No. 5,464,765 or by the direct incubation of DNA with germinating pollen, DeWet et al. in Experimental Manipulation 5 of Ovule Tissue, eds. Chapman, G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p. 197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719. The Agrobacterium system includes the use of plasmid vectors that contain defined DNA segments that integrate into the plant genomic DNA. Methods of inoculation of the plant tissue vary depending upon the plant species and the Agrobacterium delivery system. A widely LO used approach is the leaf disc procedure which can be performed with any tissue explant that provides a good source for initiation of whole plant differentiation. See, e.g., Horsch et al. in Plant Molecular Biology Manual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. A supplementary approach employs the Agrobacterium delivery system in combination with vacuum infiltration. The Agrobacterium system is especially viable in the creation of L5 transgenic dicotyledonous plants. There are various methods of direct DNA transfer into plant cells. In electroporation, the protoplasts are briefly exposed to a strong electric field. In microinjection, the DNA is mechanically injected directly into the cells using very small micropipettes. In microparticle bombardment, the DNA is adsorbed on microprojectiles such as magnesium sulfate crystals or !0 tungsten particles, and the microprojectiles are physically accelerated into cells or plant tissues. Following stable transformation plant propagation is exercised. The most common method of plant propagation is by seed. Regeneration by seed propagation, however, has the deficiency that due to heterozygosity there is a lack of uniformity in the crop, since seeds are produced by plants according to the genetic variances governed by Mendelian rules. Basically, 25 each seed is genetically different and each will grow with its own specific traits. Therefore, it is preferred that the transformed plant be produced such that the regenerated plant has the identical traits and characteristics of the parent transgenic plant. Therefore, it is preferred that the transformed plant be regenerated by micropropagation which provides a rapid, consistent reproduction of the transformed plants. 30 Micropropagation is a process of growing new generation plants from a single piece of tissue that has been excised from a selected parent plant or cultivar. This process permits the mass reproduction of plants having the preferred tissue expressing the fusion protein. The new generation plants which are produced are genetically identical to, and have all of the 23 characteristics of, the original plant. Micropropagation allows mass production of quality plant material in a short period of time and offers a rapid multiplication of selected cultivars in the preservation of the characteristics of the original transgenic or transformed plant. The advantages of cloning plants are the speed of plant multiplication and the quality and 5 uniformity of plants produced. Micropropagation is a multi-stage procedure that requires alteration of culture medium or growth conditions between stages. Thus, the micropropagation process involves four basic stages: Stage one, initial tissue culturing; stage two, tissue culture multiplication; stage three, differentiation and plant formation; and stage four, greenhouse culturing and hardening. LO During stage one, initial tissue culturing, the tissue culture is established and certified contaminant-free. During stage two, the initial tissue culture is multiplied until a sufficient number of tissue samples are produced to meet production goals. During stage three, the tissue samples grown in stage two are divided and grown into individual plantlets. At stage four, the transformed plantlets are transferred to a greenhouse for hardening where the plants' tolerance L5 to light is gradually increased so that it can be grown in the natural environment. According to some embodiments of the invention, the transgenic plants are generated by transient transformation of leaf cells, meristematic cells or the whole plant. Transient transformation can be effected by any of the direct DNA transfer methods described above or by viral infection using modified plant viruses. !0 Viruses that have been shown to be useful for the transformation of plant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus (BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation of plants using plant viruses is described in U.S. Pat. No. 4,855,237 (bean golden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese Published Application No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); and Gluzman, Y. 25 et al., Communications in Molecular Biology: Viral Vectors, Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirus particles for use in expressing foreign DNA in many hosts, including plants are described in WO 87/06261. According to some embodiments of the invention, the virus used for transient transformations is avirulent and thus is incapable of causing severe symptoms such as reduced 30 growth rate, mosaic, ring spots, leaf roll, yellowing, streaking, pox formation, tumor formation and pitting. A suitable avirulent virus may be a naturally occurring avirulent virus or an artificially attenuated virus. Virus attenuation may be effected by using methods well known in the art including, but not limited to, sub-lethal heating, chemical treatment or by directed 24 mutagenesis techniques such as described, for example, by Kurihara and Watanabe (Molecular Plant Pathology 4:259-269, 2003), Gal-on et al. (1992), Atreya et al. (1992) and Huet et al. (1994). Suitable virus strains can be obtained from available sources such as, for example, the 5 American Type culture Collection (ATCC) or by isolation from infected plants. Isolation of viruses from infected plant tissues can be effected by techniques well known in the art such as described, for example by Foster and Tatlor, Eds. "Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol 81)", Humana Press, 1998. Briefly, tissues of an infected plant believed to contain a high LO concentration of a suitable virus, preferably young leaves and flower petals, are ground in a buffer solution (e.g., phosphate buffer solution) to produce a virus infected sap which can be used in subsequent inoculations. Construction of plant RNA viruses for the introduction and expression of non-viral exogenous polynucleotide sequences in plants is demonstrated by the above references as well L5 as by Dawson, W. 0. et al., Virology (1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French et al. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990) 269:73-76; and U.S. Pat. No. 5,316,931. When the virus is a DNA virus, suitable modifications can be made to the virus itself. Alternatively, the virus can first be cloned into a bacterial plasmid for ease of constructing the !0 desired viral vector with the foreign DNA. The virus can then be excised from the plasmid. If the virus is a DNA virus, a bacterial origin of replication can be attached to the viral DNA, which is then replicated by the bacteria. Transcription and translation of this DNA will produce the coat protein which will encapsidate the viral DNA. If the virus is an RNA virus, the virus is generally cloned as a cDNA and inserted into a plasmid. The plasmid is then used to make all 25 of the constructions. The RNA virus is then produced by transcribing the viral sequence of the plasmid and translation of the viral genes to produce the coat protein(s) which encapsidate the viral RNA. In one embodiment, a plant viral polynucleotide is provided in which the native coat protein coding sequence has been deleted from a viral polynucleotide, a non-native plant viral 30 coat protein coding sequence and a non-native promoter, preferably the subgenomic promoter of the non-native coat protein coding sequence, capable of expression in the plant host, packaging of the recombinant plant viral polynucleotide, and ensuring a systemic infection of the host by the recombinant plant viral polynucleotide, has been inserted. Alternatively, the 25 coat protein gene may be inactivated by insertion of the non-native polynucleotide sequence within it, such that a protein is produced. The recombinant plant viral polynucleotide may contain one or more additional non-native subgenomic promoters. Each non-native subgenomic promoter is capable of transcribing or expressing adjacent genes or polynucleotide 5 sequences in the plant host and incapable of recombination with each other and with native subgenomic promoters. Non-native (foreign) polynucleotide sequences may be inserted adjacent the native plant viral subgenomic promoter or the native and a non-native plant viral subgenomic promoters if more than one polynucleotide sequence is included. The non-native polynucleotide sequences are transcribed or expressed in the host plant under control of the LO subgenomic promoter to produce the desired products. In a second embodiment, a recombinant plant viral polynucleotide is provided as in the first embodiment except that the native coat protein coding sequence is placed adjacent one of the non-native coat protein subgenomic promoters instead of a non-native coat protein coding sequence. L5 In a third embodiment, a recombinant plant viral polynucleotide is provided in which the native coat protein gene is adjacent its subgenomic promoter and one or more non-native subgenomic promoters have been inserted into the viral polynucleotide. The inserted non native subgenomic promoters are capable of transcribing or expressing adjacent genes in a plant host and are incapable of recombination with each other and with native subgenomic !0 promoters. Non-native polynucleotide sequences may be inserted adjacent the non-native subgenomic plant viral promoters such that the sequences are transcribed or expressed in the host plant under control of the subgenomic promoters to produce the desired product. In a fourth embodiment, a recombinant plant viral polynucleotide is provided as in the third embodiment except that the native coat protein coding sequence is replaced by a non 25 native coat protein coding sequence. The viral vectors are encapsidated by the coat proteins encoded by the recombinant plant viral polynucleotide to produce a recombinant plant virus. The recombinant plant viral polynucleotide or recombinant plant virus is used to infect appropriate host plants. The recombinant plant viral polynucleotide is capable of replication in the host, systemic spread in 30 the host, and transcription or expression of foreign gene(s) (exogenous polynucleotide) in the host to produce the desired protein. Techniques for inoculation of viruses to plants may be found in Foster and Taylor, eds. "Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in 26 Molecular Biology (Humana Pr), Vol 81)", Humana Press, 1998; Maramorosh and Koprowski, eds. "Methods in Virology" 7 vols, Academic Press, New York 1967-1984; Hill, S.A. "Methods in Plant Virology", Blackwell, Oxford, 1984; Walkey, D.G.A. "Applied Plant Virology", Wiley, New York, 1985; and Kado and Agrawa, eds. "Principles and Techniques in 5 Plant Virology", Van Nostrand-Reinhold, New York. In addition to the above, the polynucleotide of the present invention can also be introduced into a chloroplast genome thereby enabling chloroplast expression. A technique for introducing exogenous polynucleotide sequences to the genome of the chloroplasts is known. This technique involves the following procedures. First, plant cells are LO chemically treated so as to reduce the number of chloroplasts per cell to about one. Then, the exogenous polynucleotide is introduced via particle bombardment into the cells with the aim of introducing at least one exogenous polynucleotide molecule into the chloroplasts. The exogenous polynucleotides selected such that it is integratable into the chloroplast's genome via homologous recombination which is readily effected by enzymes inherent to the chloroplast. L5 To this end, the exogenous polynucleotide includes, in addition to a gene of interest, at least one polynucleotide stretch which is derived from the chloroplast's genome. In addition, the exogenous polynucleotide includes a selectable marker, which serves by sequential selection procedures to ascertain that all or substantially all of the copies of the chloroplast genomes following such selection will include the exogenous polynucleotide. Further details relating to !0 this technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507 which are incorporated herein by reference. A polypeptide can thus be produced by the protein expression system of the chloroplast and become integrated into the chloroplast's inner membrane. Since abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor in plants can involve multiple genes acting additively or in synergy 25 (see, for example, in Quesda et al., Plant Physiol. 130:951-063, 2002), the present invention also envisages expressing a plurality of exogenous polynucleotides in a single host plant to thereby achieve superior effect on abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor. Expressing a plurality of exogenous polynucleotides in a single host plant can be 30 effected by co-introducing multiple nucleic acid constructs, each including a different exogenous polynucleotide, into a single plant cell. The transformed cell can than be regenerated into a mature plant using the methods described hereinabove.

27 Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by co-introducing into a single plant-cell a single nucleic-acid construct including a plurality of different exogenous polynucleotides. Such a construct can be designed with a single promoter sequence which can transcribe a polycistronic messenger RNA 5 including all the different exogenous polynucleotide sequences. To enable co-translation of the different polypeptides encoded by the polycistronic messenger RNA, the polynucleotide sequences can be inter-linked via an internal ribosome entry site (IRES) sequence which facilitates translation of polynucleotide sequences positioned downstream of the IRES sequence. In this case, a transcribed polycistronic RNA molecule encoding the different LO polypeptides described above will be translated from both the capped 5' end and the two internal IRES sequences of the polycistronic RNA molecule to thereby produce in the cell all different polypeptides. Alternatively, the construct can include several promoter sequences each linked to a different exogenous polynucleotide sequence. The plant cell transformed with the construct including a plurality of different L5 exogenous polynucleotides, can be regenerated into a mature plant, using the methods described hereinabove. Alternatively, expressing a plurality of exogenous polynucleotides in a single host plant can be effected by introducing different nucleic acid constructs, including different exogenous polynucleotides, into a plurality of plants. The regenerated transformed plants can then be !0 cross-bred and resultant progeny selected for superior abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor traits, using conventional plant breeding techniques. Thus, the invention encompasses plants exogenously expressing (as described above) the polynucleotide(s) and/or polypeptide(s) of the invention. Once expressed within the plant 25 cell or the entire plant, the level of the polypeptide encoded by the exogenous polynucleotide can be determined by methods well known in the art such as, activity assays, Western blots using antibodies capable of specifically binding the polypeptide, Enzyme-Linked ImmunoSorbent Assay (ELISA), radio-immuno-assays (RIA), immunohistochemistry, immunocytochemistry, immunofluorescence and the like. 30 Methods of determining the level in the plant of the RNA transcribed from the exogenous polynucleotide are well known in the art and include, for example, Northern blot analysis, reverse transcription polymerase chain reaction (RT-PCR) analysis (including quantitative, semi-quantitative or real-time RT-PCR) and RNA-in situ hybridization.

28 As mentioned, the polypeptide according to some embodiments of the invention, functions as a water channel. Thus, the invention according to some embodiments encompasses functional equivalents of the polypeptide (e.g., polypeptides capable of the biological activity of a water channel) which can be identified by functional assays (e.g., being 5 capable of transporting water in a plant) using e.g., a cell-swelling assay (Meng, Q. X. et al. 2008. Cell Physiol Biochem, 21. pp. 123-128). The polynucleotides and polypeptides described hereinabove can be used in a wide range of economical plants, in a safe and cost effective manner. The effect of the transgene (the exogenous polynucleotide encoding the polypeptide) on 10 abiotic stress tolerance, water use efficiency, fertilizer use efficiency, growth, biomass, yield and/or vigor can be determined using known methods. Abiotic stress tolerance - Transformed (i.e., expressing the transgene) and non transformed (wild type) plants are exposed to an abiotic stress condition, such as water deprivation, suboptimal temperature (low temperature, high temperature), nutrient deficiency, 15 nutrient excess, a salt stress condition, osmotic stress, heavy metal toxicity, anaerobiosis, atmospheric pollution and UV irradiation. Salinity tolerance assay - Transgenic plants with tolerance to high salt concentrations are expected to exhibit better germination, seedling vigor or growth in high salt. Salt stress can be effected in many ways such as, for example, by irrigating the plants with a hyperosmotic 20 solution, by cultivating the plants hydroponically in a hyperosmotic growth solution (e.g., Hoagland solution), or by culturing the plants in a hyperosmotic growth medium [e.g., 50 % Murashige-Skoog medium (MS medium)]. Since different plants vary considerably in their tolerance to salinity, the salt concentration in the irrigation water, growth solution, or growth medium can be adjusted according to the specific characteristics of the specific plant cultivar or 25 variety, so as to inflict a mild or moderate effect on the physiology and/or morphology of the plants (for guidelines as to appropriate concentration see, Bernstein and Kafkafi, Root Growth Under Salinity Stress In: Plant Roots, The Hidden Half 3rd ed. Waisel Y, Eshel A and Kafkafi U. (editors) Marcel Dekker Inc., New York, 2002, and reference therein). For example, a salinity tolerance test can be performed by irrigating plants at different 30 developmental stages with increasing concentrations of sodium chloride (for example 50 mM, 100 mM, 200 mM, 400 mM NaCl) applied from the bottom and from above to ensure even dispersal of salt. Following exposure to the stress condition the plants are frequently monitored until substantial physiological and/or morphological effects appear in wild type plants. Thus, 29 the external phenotypic appearance, degree of wilting and overall success to reach maturity and yield progeny are compared between control and transgenic plants. Quantitative parameters of tolerance measured include, but are not limited to, the average wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. 5 Transformed plants not exhibiting substantial physiological and/or morphological effects, or exhibiting higher biomass than wild-type plants, are identified as abiotic stress tolerant plants. Osmotic tolerance test - Osmotic stress assays (including sodium chloride and mannitol assays) are conducted to determine if an osmotic stress phenotype was sodium chloride-specific or if it was a general osmotic stress related phenotype. Plants which are tolerant to osmotic LO stress may have more tolerance to drought and/or freezing. For salt and osmotic stress germination experiments, the medium is supplemented for example with 50 mM, 100 mM, 200 mM NaCl or 100 mM, 200 mM NaCl, 400 mM mannitol. See also Example 5 of the Examples section which follows. Drought tolerance assay/Osmoticum assay - Tolerance to drought is performed to L5 identify the genes conferring better plant survival after acute water deprivation. To analyze whether the transgenic plants are more tolerant to drought, an osmotic stress produced by the non-ionic osmolyte sorbitol in the medium can be performed. Control and transgenic plants are germinated and grown in plant-agar plates for 4 days, after which they are transferred to plates containing 500 mM sorbitol. The treatment causes growth retardation, then both control and !0 transgenic plants are compared, by measuring plant weight (wet and dry), yield, and by growth rates measured as time to flowering. Conversely, soil-based drought screens are performed with plants overexpressing the polynucleotides detailed above. Seeds from control Arabidopsis plants, or other transgenic plants overexpressing the polypeptide of the invention are germinated and transferred to pots. 25 Drought stress is obtained after irrigation is ceased accompanied by placing the pots on absorbent paper to enhance the soil-drying rate. Transgenic and control plants are compared to each other when the majority of the control plants develop severe wilting. Plants are re-watered after obtaining a significant fraction of the control plants displaying a severe wilting. Plants are ranked comparing to controls for each of two criteria: tolerance to the drought conditions and 30 recovery (survival) following re-watering. Cold stress tolerance - To analyze cold stress, mature (25 day old) plants are transferred to 4 'C chambers for 1 or 2 weeks, with constitutive light. Later on plants are moved back to greenhouse. Two weeks later damages from chilling period, resulting in growth retardation and 30 other phenotypes, are compared between both control and transgenic plants, by measuring plant weight (wet and dry), and by comparing growth rates measured as time to flowering, plant size, yield, and the like. Heat stress tolerance - Heat stress tolerance is achieved by exposing the plants to 5 temperatures above 34 'C for a certain period. Plant tolerance is examined after transferring the plants back to 22 'C for recovery and evaluation after 5 days relative to internal controls (non-transgenic plants) or plants not exposed to neither cold or heat stress. Germination tests - Germination tests compare the percentage of seeds from transgenic plants that could complete the germination process to the percentage of seeds from control LO plants that are treated in the same manner. Normal conditions are considered for example, incubations at 22 'C under 22-hour light 2-hour dark daily cycles. Evaluation of germination and seedling vigor is conducted between 4 and 14 days after planting. The basal media is 50 % MS medium (Murashige and Skoog, 1962 Plant Physiology 15, 473-497). Germination is checked also at unfavorable conditions such as cold (incubating at L5 temperatures lower than 10 'C instead of 22 C) or using seed inhibition solutions that contain high concentrations of an osmolyte such as sorbitol (at concentrations of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM, and up to 1000 mM) or applying increasing concentrations of salt (of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM NaCl). Water use efficiency - can be determined as the biomass produced per unit !0 transpiration. To analyze WUE, leaf relative water content can be measured in control and transgenic plants. Fresh weight (FW) is immediately recorded; then leaves are soaked for 8 hours in distilled water at room temperature in the dark, and the turgid weight (TW) is recorded. Total dry weight (DW) is recorded after drying the leaves at 60 'C to a constant weight. Relative water content (RWC) is calculated according to the following Formula I: 25 Formula I (FW - DW/TW - DW) x 100 Fertilizer use efficiency - To analyze whether the transgenic plants are more responsive to fertilizers, plants are grown in agar plates or pots with a limited amount of fertilizer, as described, for example, in Example 6, hereinbelow and in Yanagisawa et al (Proc Natl Acad 30 Sci U S A. 2004; 101:7833-8). The plants are analyzed for their overall size, time to flowering, yield, protein content of shoot and/or grain. The parameters checked are the overall size of the mature plant, its wet and dry weight, the weight of the seeds yielded, the average seed size and the number of seeds produced per plant. Other parameters that may be tested are: the 31 chlorophyll content of leaves (as nitrogen plant status and the degree of leaf verdure is highly correlated), amino acid and the total protein content of the seeds or other plant parts such as leaves or shoots, oil content, etc. Similarly, instead of providing nitrogen at limiting amounts, phosphate or potassium can be added at increasing concentrations. Again, the same parameters 5 measured are the same as listed above. In this way, nitrogen use efficiency (NUE), phosphate use efficiency (PUE) and potassium use efficiency (KUE) are assessed, checking the ability of the transgenic plants to thrive under nutrient restraining conditions. Nitrogen determination - The procedure for N (nitrogen) concentration determination in the structural parts of the plants involves the potassium persulfate digestion method to LO convert organic N to N03- (Purcell and King 1996 Argon. J. 88:111-113, the modified Cd mediated reduction of N03- to NO2 (Vodovotz 1996 Biotechniques 20:390-394) and the measurement of nitrite by the Griess assay (Vodovotz 1996, supra). The absorbance values are measured at 550 nm against a standard curve of NaNO 2 . The procedure is described in details in Samonte et al. 2006 Agron. J. 98:168-176. L5 Grain protein concentration - Grain protein content (g grain protein m-2 ) is estimated as the product of the mass of grain N (g grain N m-2 ) multiplied by the N/protein conversion ratio of k-5.13 (Mosse 1990, supra). The grain protein concentration is estimated as the ratio of grain protein content per unit mass of the grain (g grain protein kg- 1 grain). Oil content - The oil content of a plant can be determined by extraction of the oil from !0 the seed or the vegetative portion of the plant. Briefly, lipids (oil) can be removed from the plant (e.g., seed) by grinding the plant tissue in the presence of specific solvents (e.g., hexane or petroleum ether) and extracting the oil in a continuous extractor. Indirect oil content analysis can be carried out using various known methods such as Nuclear Magnetic Resonance (NMR) Spectroscopy, which measures the resonance energy absorbed by hydrogen atoms in the liquid 25 state of the sample [See for example, Conway TF. and Earle FR., 1963, Journal of the American Oil Chemists' Society; Springer Berlin / Heidelberg, ISSN: 0003-021X (Print) 1558-9331 (Online)]; the Near Infrared (NI) Spectroscopy, which utilizes the absorption of near infrared energy (1100-2500 nm) by the sample; and a method described in WO/2001/023884, which is based on extracting oil a solvent, evaporating the solvent in a gas stream which forms oil 30 particles, and directing a light into the gas stream and oil particles which forms a detectable reflected light. The plant vigor can be calculated by the increase in growth parameters such as leaf area, fiber length, rosette diameter, plant fresh weight and the like per time.

32 The growth rate can be measured using digital analysis of growing plants. For example, images of plants growing in greenhouse on plot basis can be captured every 3 days and the rosette area can be calculated by digital analysis. Rosette area growth is calculated using the difference of rosette area between days of sampling divided by the difference in days between 5 samples. Measurements of seed yield can be done by collecting the total seeds from 8-16 plants together, weighting them using analytical balance and dividing the total weight by the number of plants. Seed per growing area can be calculated in the same manner while taking into account the growing area given to a single plant. Increase seed yield per growing area could be LO achieved by increasing seed yield per plant, and/or by increasing number of plants capable of growing in a given area. Evaluation of the seed yield per plant can be done by measuring the amount (weight or size) or quantity (i.e., number) of dry seeds produced and harvested from 8-16 plants and divided by the number of plants. L5 Evaluation of growth rate can be done by measuring plant biomass produced, rosette area, leaf size or root length per time (can be measured in cm2 per day of leaf area). Fiber length can be measured using fibrograph. The fibrograph system was used to compute length in terms of "Upper Half Mean" length. The upper half mean (UHM) is the average length of longer half of the fiber distribution. The fibrograph measures length in span !0 lengths at a given percentage point (Hypertext Transfer Protocol://World Wide Web (dot) cottoninc (dot) com/ClassificationofCotton/?Pg=4#Length). Thus, the present invention is of high agricultural value for promoting the yield of commercially desired crops (e.g., biomass of vegetative organ such as poplar wood, or reproductive organ such as number of seeds or seed biomass). 25 As used herein the term "about" refers to ± 10 %. The terms "comprises", "comprising", "includes", "including", "having" and their conjugates mean "including but not limited to". The term "consisting of means "including and limited to". The term "consisting essentially of" means that the composition, method or structure 30 may include additional ingredients, steps and/or parts, but only if the additional ingredients, steps and/or parts do not materially alter the basic and novel characteristics of the claimed composition, method or structure.

33 As used herein, the singular form "a", "an" and "the" include plural references unless the context clearly dictates otherwise. For example, the term "a compound" or "at least one compound" may include a plurality of compounds, including mixtures thereof. Throughout this application, various embodiments of this invention may be presented in 5 a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have LO specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range. Whenever a numerical range is indicated herein, it is meant to include any cited numeral (fractional or integral) within the indicated range. The phrases "ranging/ranges L5 between" a first indicate number and a second indicate number and "ranging/ranges from" a first indicate number "to" a second indicate number are used herein interchangeably and are meant to include the first and second indicated numbers and all the fractional and integral numerals therebetween. As used herein the term "method" refers to manners, means, techniques and procedures !0 for accomplishing a given task including, but not limited to, those manners, means, techniques and procedures either known to, or readily developed from known manners, means, techniques and procedures by practitioners of the chemical, pharmacological, biological, biochemical and medical arts. It is appreciated that certain features of the invention, which are, for clarity, described in 25 the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable subcombination or as suitable in any other described embodiment of the invention. Certain features described in the context of various embodiments are not to be considered essential 30 features of those embodiments, unless the embodiment is inoperative without those elements. Various embodiments and aspects of the present invention as delineated hereinabove and as claimed in the claims section below find experimental support in the following examples.

34 EXAMPLES Reference is now made to the following examples, which together with the above descriptions illustrate some embodiments of the invention in a non limiting fashion. Generally, the nomenclature used herein and the laboratory procedures utilized in the 5 present invention include molecular, biochemical, microbiological and recombinant DNA techniques. Such techniques are thoroughly explained in the literature. See, for example, "Molecular Cloning: A laboratory Manual" Sambrook et al., (1989); "Current Protocols in Molecular Biology" Volumes 1-111 Ausubel, R. M., ed. (1994); Ausubel et al., "Current Protocols in Molecular Biology", John Wiley and Sons, Baltimore, Maryland (1989); Perbal, LO "A Practical Guide to Molecular Cloning", John Wiley & Sons, New York (1988); Watson et al., "Recombinant DNA", Scientific American Books, New York; Birren et al. (eds) "Genome Analysis: A Laboratory Manual Series", Vols. 1-4, Cold Spring Harbor Laboratory Press, New York (1998); methodologies as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and 5,272,057; "Cell Biology: A Laboratory Handbook", Volumes I-III Cellis, J. E., L5 ed. (1994); "Current Protocols in Immunology" Volumes I-III Coligan J. E., ed. (1994); Stites et al. (eds), "Basic and Clinical Immunology" (8th Edition), Appleton & Lange, Norwalk, CT (1994); Mishell and Shiigi (eds), "Selected Methods in Cellular Immunology", W. H. Freeman and Co., New York (1980); available immunoassays are extensively described in the patent and scientific literature, see, for example, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; !0 3,850,578; 3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521; "Oligonucleotide Synthesis" Gait, M. J., ed. (1984); "Nucleic Acid Hybridization" Hames, B. D., and Higgins S. J., eds. (1985); "Transcription and Translation" Hames, B. D., and Higgins S. J., Eds. (1984); "Animal Cell Culture" Freshney, R. I., ed. (1986); "Immobilized Cells and Enzymes" IRL Press, (1986); "A 25 Practical Guide to Molecular Cloning" Perbal, B., (1984) and "Methods in Enzymology" Vol. 1-317, Academic Press; "PCR Protocols: A Guide To Methods And Applications", Academic Press, San Diego, CA (1990); Marshak et al., "Strategies for Protein Purification and Characterization - A Laboratory Course Manual" CSHL Press (1996); all of which are incorporated by reference as if fully set forth herein. Other general references are provided 30 throughout this document. The procedures therein are believed to be well known in the art and are provided for the convenience of the reader. All the information contained therein is incorporated herein by reference.

35 EXAMPLE 1 IDENTIFICATION OF AQP GENES USING DIGITAL EXPRESSION ANALYSIS AND CROSS-SPECIES COMPARATIVE GENOMIC The large number of AQPs in plants and the contradictory results obtained when AQPs 5 were overexpressed in plants demonstrate the need to selectively identify the AQP genes which can improve water use efficiency (WUE) in plants, lead to increased yield and biomass under abiotic stress as well as under favorable conditions. Under unfavorable stress conditions, some biological activities of the plant are stopped or reduced, while others, not earlier active, initiate. Still, some of the activities, which are vital LO for plant survival, are maintained. One hypothesis is that key genes needed for plants to maintain vital activities under unfavorable conditions would be active under broad spectrum of biotic and abiotic stresses. To test this hypothesis and to identify the key AQP genes having the potential to improve plant performance under different biotic and/or abiotic stress conditions (e.g., salt or L5 drought stress) a combination of digital expression analysis (also known as Electronic Northern blot) and cross-species comparative genomics was performed. The database used was available from NCBI (Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/dbEST/) and included 7.2 million expressed sequence tags (ESTs) from 1,195 relevant EST's libraries originated from 15 different species, including both monocot and dicot species, !0 namely: Arabidopsis, barley, Brassica rapa, cotton, grape, maize, medicago, poplar, potato, rice, sorghum, soybean, sugarcane, tomato and wheat. Tomato plants were selected as a model plant based on the high quality tomato database from several tomato species which can be used for data-mining and the present inventors' experience in using the tomato genome as a model plant. In addition, the relatively high salt 25 tolerance exhibited by various tomato species makes the tomato genome an excellent candidate for identifying new stress tolerance mechanisms. Moreover, tomato is not only used as a model plant for genetic studies, it is also used as an important crop with well-defined yield parameters, which can be used to distinguish between genes affecting abiotic-stress tolerance and genes preventing yield loss under abiotic-stress conditions. 30 Gene analysis and data mining - For gene analysis and data mining the bioinformatic filtering approach used had three phases: 1. Clustering and assembly: EST and mRNA sequences of each of the 15 species were extracted from GenBank versions 157, 160, 161, 162, 164, 165, 166, clustered and assembled 36 using Compugen's LEADS clustering and assembly platform (Compugen Ltd., Tel Aviv, Israel; Yelin et. al. 2003, Nature Biotechnology 21, 379-85). Automatically extracted EST library annotations were manually accurated and classified by anatomy, developmental stage, abiotic/biotic stress treatment and cultivars. The results were loaded into Oracle database. The 5 predicted proteins were then annotated using InterPro(2) (Hypertext Transfer Protocol://World Wide Web (dot) ebi (dot) ac (dot) uk/interpro/). 2. Selection of clusters - All clusters that contained the Major intrinsic protein domain (IPR000425) were selected for further analysis (n = 1,114). 3. Obtaining expression profile of the clusters - By digital expression approach the LO expression profile of all clusters was obtained in terms of plant anatomy (i.e., in what tissues/organs the gene was expressed), developmental stage (i.e., the developmental stages at which a gene can be found) and profile of treatment (provides the physiological conditions under which a gene is expressed such as drought, cold, pathogen infection, etc). Digital expression computations was calculated as follows: over-expression fold was L5 computed as m/ (n* M/N), where "N" is total number of ESTs of specific organism; "M is number of ESTs in a given library/tissue/category; "n" is total number of ESTs in a given contig; "m" is the number of ESTs from the library/tissue/category in the contig; P-value was computed using Exact Fisher Test statistic. The combined P-value for over-expression in both Root and Abiotic stresses conditions was computed as 1 - (1-pl) x (1-p2). 1,114 different AQP !0 genes were identified in the inter species transcriptional databases. For the data mining process, the present inventors used a combination of two approaches: selection of AQP clusters showing significant over expression (EST distribution versus normal is more than two folds; statistical significance of over-expression - p Value < 0.05) either in roots compared to shoots or under various abiotic stresses (including drought, cold, salinity, heat, chemical treatments, etc.), 25 compared to non stress control. It was found that ESTs of about 9 % of the AQP genes were significantly overrepresented in roots and 3.5 % of them were induced under different abiotic stresses. AQP genes which are highly overrepresented in roots were selected since plants with an efficient root system are expected to capture more water from a drying soil. In addition, AQP genes which are overrepresented in various abiotic stresses such as nutrient deficiency, 30 heat, salinity and heavy metal stresses and biotic stresses such as application of elicitors and pathogens were selected considering that they can provide high tolerance to a wide spectrum of stresses.

37 The same set of 1,114 AQPs was classified according to the accepted groups known in the literature: first into the four major sub-groups: PIPs, TIPs, NIPs and SIPs, and a second classification divided these four sub-groups into eleven sub-groups according to their homology in amino acid sequences. A Fisher's exact test was then used to identify subgroups having 5 significant EST over-presentation both in roots and upon exposure to different abiotic stresses. As shown in Table 1, hereinbelow, from the eleven subgroups, only the TIP2 subgroup showed a significant EST overrepresentation both in roots and upon exposure to abiotic stresses (P-value 1.7 X 10- 5 and 1.6 X 10-3, respectively). LO Table 1 AQP type distribution and over-expression in roots and abiotic stresses Roots Exposure to abiotic stresses AQP Total No. of No. of over- % over- P- No. of over- % over- P type genes in expressed expressed/all value expressed expressed/all value database genes genes PIPI 243 26 10.7 0.13 10 4.1 0.34 PIP2 336 25 7.4 0.87 12 3.6 0.53 PIP3 11 0 0.0 1 0 0 1 SIP1 39 0 0.0 1 0 0 1 SIP2 16 0 0.0 1 0 0 1 TIP1 152 11 7.2 0.8 3 2 0.92 TIP2 101 22 21.8 1705E 10 9.9 . TIP3 29 0 0.0 1 0 0 1 TIP4 48 5 10.4 0.41 1 2.1 0.83 TIP5 3 0 0.0 1 0 0 1 NIP 136 8 5.9 0.93 3 2.2 0.88 Total 1114 97 39 Table 1. These results suggest that over-expression and/or protein over-accumulation of the Tip2 15 subgroup can improve plant water use efficiency, ABST and yield. Genes of the Tip2 subgroup are highly expressed in roots and in abiotic stresses - As shown in Table 1, hereinabove, the TIP2 subgroup (or subfamily) is highly expressed in roots and in abiotic stresses. The TIP2 subgroup is found in 38 plant species and other organisms (nucleic acid SEQ ID NOs: 1, 2, 19, 20-22; Table 2), available in public databases [Hypertext 20 Transfer Protocol://World Wide Web (dot) ncbi (dot) nlm (dot) nih (dot) gov/dbEST/]. In tomato, the TIP2 gene was highly expressed in roots (6 fold, p < 1.01 E-24) and in both biotic (2 fold, p < 4.6 E-02) and abiotic stresses (4.5 fold, p < E-02) (data not shown). Identification of a short consensus sequence of the Tip2 sub-family - While comparing the consensus amino-acid sequences of Aquaporins, a short consensus sequence was 25 identified which is unique to proteins of the Tip2 sub-family. The present inventors have 38 suggested that this motif has an important role in managing water use efficiency (WUE), and when over-expressed in a plant can confer ABST and improved yield. The amino-acid consensus sequence identified is TLXFXFAGVGS (SEQ ID NO:2826), wherein X stands for any amino acid. 5 In addition, other genes of the aquaporin gene family were identified by bioinformatics tools as improving ABST and yield, based on combined digital gene expression profile in roots, tissues with low water levels (such as seed and pollen) and under abiotic stress conditions. These include SEQ ID NOs: 3-18, 23-26 (Table 2). LO Table 2 Identified Aquaporin Genes (Poy ucet e) Gene name Cluster name Organism SEQ IDNO 1 MAB54 tomatolgb1641BG125449 tomato 27 2 MAB55 tomatolgb1641BG134896 tomato 28 3 MAB56 tomatolgb1641AW218990 tomato 29 4 MAB57 tomatolgb1641AA824812 tomato 30 6 MAB58 tomatolgb1641AW934056 tomato 32 7 MAB69 tomatolgb1641AI637360 tomato 33 8 MAB70 tomatolgb1641BG133531 tomato 34 9 MAB71 tomatolgb1641BG629975 tomato 35 10 MAB72 tomatolgb1641BG136017 tomato 36 11 MAB73 tomatolgb1641BG131871 tomato 37 12 MAB74 tomatolgb1641AI775489 tomato 38 13 MAB75 tomatolgb1641BG136239 tomato 39 14 MAB76 tomatolgb1641BG134058 tomato 40 15 MAB77 tomatolgb1641BG629900 tomato 41 16 MAB78 tomatolgb1641BG130774 tomato 42 17 MAB79 tomatolgb1641BG124486 tomato 43 18 MAB80 tomatolgb1641AI483521 tomato 44 19 MAB81 tomatolgb1641CO751453 tomato 45 20 MAB115 barleylgbl57.21BF626376 barley 46 22 MAB117 barleylgb157.21BE412516 barley 48 23 MAB119 tomatoIgb1641BG134199 tomato 49 24 MAB176 tomatoIgb1641CO635830 tomato 50 25 MAB177 tomatoIgb1641CO751496 tomato 51 26 MAB178 tomatoIgb1641CO751374 tomato 52 Table 2. Sequences which are homologous [showing at least 80 % protein sequence identity on 15 80 % of the global hit or query length, as calculated using BlastP and tBlastN algorithms of the National Center of Biotechnology Information (NCBI)] or orthologues of the AQP genes described in Table 2, and are expected to possess the same role in ABST and yield improvement in plants, are disclosed in Table 3 hereinbelow (SEQ ID NOs:6, 215-1101 and 1138-1400; Table 3). In addition, Table 3 also includes homologous and orthologues of the AQP TIP2 subfamily 39 (SEQ ID NOs:21, 53-214, 1102-1137) and additional homologous and orthologues (SEQ ID NOs:2844-305 1). Table 3 5 Polynucleotide and polypeptide sequences of AQP homologous and orthologous Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 53 applelgb157.3|C apple 1401 27 84 92.3387 100 blastp N883304_TI 097 54 applegb573|C apple 1402 27 83 100 100 blastp 55 3aquiegialgb157. aquilegia 1403 27 85 100 100 blastp arabidopsisgbl6 arabidop 99.1935 56 51AT3G16240T sis 1404 27 81 484 98.8 blastp 1 57 artemisialgbl641 artemisia 1405 27 80 98.7903 99.1902 blastp EY035829_TI 226 834 58 artemisiagb1641 artemisia 1406 27 85 62.9032 100 blastp 58 EY113320_TI atmsa 10 7 8 258 59 artemisialgbl641 artemisia 1407 27 81 98.7903 99.1902 blastp EY070770_TI 226 834 60 avocadolgbI641C avocado 1408 27 84 50 100 blastp V002132_TI bjuncealgb1641 61 EVGN00333108 b-juncea 1409 27 83 100 100 blastp 491419_TI bjuncealgb1641 53.6290 97.0588 62 EVGN00503709 b-juncea 1410 27 82 323 235 blastp 641655_TI bjuncealgb1641 63.7096 63 EVGN01003711 b-juncea 1411 27 84 774 100 blastp 220829_TI 64 b-oleracealgbI6 bolerac 1412 27 84 100 100 blastp 1|AM059585_TI ea 65 b-oleracealgbI6 bolerac 1413 27 82 100 100 blastp 1|AM385334_TI ea 66 b-oleracealgbI6 bolerac 1414 27 81 100 100 blastp 1|AM385915_TI ea 67 bjrapalgbI621B b rapa 1415 27 82 100 100 blastp G543171TI b 68 bjrapalgbI62|B b rapa 1416 27 83 100 100 blastp G543223_TI 69 b-rapalgbl621L3 brapa 1417 27 81 100 100 blastp 7478_TI _ 70 bananalgbI60|D banana 1418 27 83 76.6129 100 blastp N238689_TI 032 71 beanIgbI64ICB5 bean 1419 27 83 98.7903 98.7903 blastp 40614_TI 226 226 72 canolaIgbI6iIEV canola 1420 27 84 100 100 blastp 092237_TI 73 canolaIgbI6IICN canola 1421 27 81 100 100 blastp 828178_TI ___ 74 canolaIgbI6IICX canola 1422 27 82 100 100 blastp 188169_TI ________________ ___________ 40 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 75 canolaIgbI6I|CD canola 1423 27 81 100 100 blastp 840590_TI 76 cassavagbI64lC cassava 1424 27 85 100 100 blastp K650415_TI 77 castorbeanlgbI6 castorbea 1425 27 87 100 100 blastp 0IEE254645_TI n 78 centaurealgbI611 centaure 1426 27 84 95.9677 84.3971 blastp EH725826_TI a 419 631 79 centaurealgbI611 centaure 1427 27 88 89.1129 97.7876 blastp EL932474_TI a 032 106 80 cherrylgbI57 .21E cherry 1428 27 80 69.3548 100 blast E488049_TI 387 81 cichoriumlgbI61 cichoriu 1429 27 80 100 92.8571 blastp IDT211633_Ti m 429 82 cichoriumlgbI61 cichoriu 1430 27 87 100 100 blastp IEH672622_TI m 83 citruslgb157.21C citrus 1431 27 88 98.7903 99.1902 blastp X663669_TI 226 834 84 citrusgbl57.21C citrus 1432 27 88 98.7903 99.1902 blast F417983_TI 226 834 85 citruslgb157.21C citrus 1433 27 88 98.7903 99.1902 blastp K665344_TI 226 834 86 citruslgb157.21C citrus 1434 27 87 82.2580 100 blast K665344_T2 645 87 cloverIgbI621BB clover 1435 27 81 69.7580 100 blast 908328_TI 645 88 cottongbl641AF cotton 1436 27 87 100 100 blastp 89 cowpea 166F cowpea 1437 27 84 100 100 blastp 90 cowpealgbI66|F cowpea 1438 27 83 98.7903 98.7903 blastp C457059_TI 226 226 91 dandeliongbI611 dandelio 1439 27 85 100 100 blastp DY818755_TI n 92 gingerlgbI641DY ginger 1440 27 80 98.3870 99.1836 blastp 358186_TI 968 735 93 gingergbl641DY ginger 1441 27 80 98.3870 99.1836 blastp 3518661 g 968 735 94 iceplantib1641A iceplant 1442 27 81 100 100 blastp 95 i 5moealgbi57.2 ipomoea 1443 27 87 100 100 blastp Iett5c7g630 T2I 96 DW07 363_T1 lettuce 1444 27 87 100 100 blastp 97 lettuce Igbl157.21 lettuce 1445 27 85 50.8064 90.6474 blastp DW074363_T2 516 82 98 D I 132_T1 lettuce 1446 27 87 100 100 blastp 99 D 760_T1 lettuce 1447 27 87 100 100 blastp 100 D I 999_T1 lettuce 1448 27 87 100 100 blastp 101 lotus1gb5721B lotus 1449 27 84 100 100 blastp 41 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 102 lotuslgb157.21BF lotus 1450 27 86 98.7903 98.7951 blastp 177457_TI 226 807 103 medicagolgbl57. medicag 1451 27 83 89.1129 94.8497 blastp 21AI974300_T o 032 854 104 medicagogbl57. medicag 1452 27 84 100 100 blastp 21AA660400_TI o nicotiana bentha nicotiana 98.7903 98.7903 105 mianalgbi621CN _bentha 1453 27 86 226 226 blast 741988_TI miana nicotiana bentha nicotiana 106 mianalgbi621CN _bentha 1454 27 92 100 100 blastp 655366_TI miana nicotiana bentha nicotiana 98.7903 98.7903 107 mianalgb1621CN _bentha 1455 27 89 226 226 blast 741998_TI miana nicotiana bentha nicotiana 108 mianalgb1621CN _bentha 1456 27 93 100 100 blastp 742343_TI miana 109 U45214_T1|B peach 1457 27 82 100 100 blastp 110 peppergb57.21 pepper 1458 27 84 61.6935 100 blastp C0776446_TI pepr 1527 8 484 111 pepperlgb157.21 pepper 1459 27 86 63.3064 100 blastp CA518313 TI pepr 1527 8 516 10 112 periwinklelgbl6 periwink 1460 27 88 99.1935 99.1935 blastp 41EG555051_TI le 484 484 113 petunialgbl57.21 petunia 1461 27 84 63.7096 85.2517 tblastn CV296219_TI 774 986 114 1b157.21B poplar 1462 27 86 100 100 blastp 115 poplarlgb157.21A poplar 1463 27 83 61.6935 100 blastp 1166943_T2 484 116 1b15721A poplar 1464 27 85 100 100 blastp 117 poplarlgbl57.21B poplar 1465 27 89 79.0322 100 blastp 1127662_Ti 581 118 potato gb15721B potato 1466 27 97 100 100 blastp 119 potatolgbl57.21B potato 1467 27 97 50.8064 96.1832 blast Q513382_T2 516 061 120 radishlgbl641EV radish 1468 27 82 92.3387 100 blastp 538411_TI 097 121 radishlgb1641AB radish 1469 27 81 100 100 blastp 010416_TI 7259dihb145_Ti radish 1470 27 82 100 100 blastp 123 radishlgbl641EV radish 1471 27 81 100 100 blastp 527946_TI 124 roselgb157.21BQ rose 1472 27 85 85.0806 95.0450 blast 104096_TI 452 45 125 safflowergb162 safflower 1473 27 87 100 100 blastp EL374001 s o 120 0t 126 safflowerlgb1621 safflower 1474 27 83 100 100 blastp EL406178_TI ________________ ___________ 42 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 127 sesamelgbl57.21 sesame 1475 27 81 61.2903 86.3636 tblastn BU668161_TI 226 364 128 soybeanlgbl66|A soybean 1476 27 84 98.7903 98.7903 blastp W349399_TI 226 226 129 soybeanlgbl66|C soybean 1477 27 85 75.4032 100 blastp D416937_TI 258 130 soybeanlgbl66|C soybean 1478 27 84 98.7903 98.7903 blastp A786095_TI 226 226 131 soWbeanTgbl661A soybean 1479 27 81 100 100 blastp 132 sprucelgbl62|CO spruce 1480 27 80 98.7903 98.4 blast 216479_TI 226 133 strawberrylgbl6 strawberry 1481 27 82 100 100 blastp 41DV438565T1 y 134 sunflowerlgbl621 sunflowe 1482 27 82 100 100 blastp X95952_TI r 135 sunflowerlgbl621 sunflowe 1483 27 83 100 100 blastp CD847513_TI r 136 sunflowerlgbl621 sunflowe 1484 27 84 100 100 blastp CD845750_TI r 137 sunflowerlgbl621 sunflowe 1485 27 86 100 100 blastp CD848081_TI r 138 sunflowerlgbl621 sunflowe 1486 27 83 100 100 blastp CD849577_TI r 139 tobaccolgb162|C tobacco 1487 27 88 100 100 blastp V016921 TI 140 tobaccolgb162|C tobacco 1488 27 93 100 100 blastp V018684_TI 141 tobaccolgb1621C tobacco 1489 27 91 100 100 blastp V019641_TI 12 tomatolgbi64lA No88.3610 tban 142 tm g641A tomato predicted 27 83 50 1 tblastn - protein 143 triphysarialgbl6 triphysar 1490 27 81 100 100 blastp 41EX999390_TI ia 144 triphysarialgbl6 triphysar 1491 27 80 100 100 blastp 41BM356478_TI ia 145 triphysarialgbl6 triphysar 1492 27 81 81.0483 98.0487 blastp 41BM356478_T2 ia 871 805 146 3auegialgb157. aquilegia 1493 28 86 100 100 blastp arabidopsislgbl6 arabi 147 51AT5G47450_T idop 1494 28 82 98.8 98.8 blastp 1 arabidopsislgbl6 arabi 148 51AT4G17340_T idop 1495 28 85 99.6 99.6 blastp 1 149 artemisialgbl641 artemisia 1496 28 83 69.2 100 blastp EY080612_TI 150 bjrapalgb1621E b rapa 1497 28 84 95.2 99.1666 blastp X104899_TI - 667 151 canolalgb1611EE canola 1498 28 85 95.2 94.8207 blastp 430505_TI 171 152 canolalgbl611D canola 1499 28 82 98.8 98.8 blastp Y017904_TI ______________________________ 43 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 153 canolaIgb1611EL canola 1500 28 84 99.6 99.6 blastp 590702_TI 154 canolaIgbI6I|CD canola 1501 28 85 99.6 99.6 blastp 818320_-TI 155 cassavagbI641D cassava 1502 28 81 100 100 blastp B923860_TI 156 centaurealgbI611 centaure 1503 28 84 98.8 99.1935 blastp EL932179_TI a 484 157 centaurealgbI611 centaure 1504 28 82 87.6 88.9795 blastp EH718862_TI a 918 158 cichorium~gbl61 cichoriu 1505 28 86 88.8 64.7230 tblastn IEH689841_TI m 321 159 citrusgb1572C citrus 1506 28 84 100 100 blastp 160 citruslgbl57.21C citrus 1507 28 82 95.6 75.2360 tblastn 0912449_TI 965 161 cottongb564DV cotton 1508 28 88 50.8 100 blastp 162 cottongbl641CD cotton 1509 28 86 100 100 blastp 163 dandelionlgbl611 dandelio 1510 28 84 100 100 blastp DY827614_TI n 164 dandelionlgbI611 dandelio 1511 28 86 100 100 blastp DY822865_TI n 165 dandelionlgbI611 dandelio 1512 28 86 100 100 blast DY819043_TI n 166 iceplantlgbl641A iceplant 1513 28 82 82 99.5145 blastp F133533_TI 631 167lettcec54l2g85 10510 bl2t 167 tc5 721 lettuce 1514 28 85 100 100 blastp 19ete 16 28057.21 b 168 e45 7.2T lettuce 1515 28 85 100 100 blastp 172lettcec59l2g86 10510 bl2t 173520 8T etcI52 8 8 00 10 bat 169 u46157.Ti lettuce 1516 28 84 100 100 blastp 170 DWu8e 72T lettuce 1517 28 85 85.6 100 blastp 171 W0756i ,_Ti lettuce 1518 28 86 100 100 blastp 17728 lettuce 1519 28 86 100 100 blastp 173 e157.2T lettuce 1520 28 84 100 100 blastp 174 tIgI27_Ti lettuce 1521 28 85 100 100 blastp 175 lettucelgbI57 '21 lettuce 1522 28 85 100 100 blastp DW079798_TI 176 DW079egb54'_Ti lettuce 1523 28 85 100 100 blastp 177 DW14e~b737'_Ti lettuce 1524 28 85 100 100 blastp 178 letueW0 527.2Ti lettuce 1525 28 83 100 100 blastp 19 lettucelgbI57 '.21 ltue 12 8 8 0 0 19 DW105592 TI letc 156 286 00 00 bst 44 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 180 lettucegbI57 21 lettuce 1527 28 85 100 100 blastp 181 D e 15T1 lettuce 1528 28 86 100 100 blast DW155534TI 182 lettucelgbI57 '21 lettuce 1529 28 85 100 100 blastp 183 lettuce 1530 28 84 100 100 blast DW178166_TI 184 lotusgb57.21A lotus 1531 28 80 53.2 100 blastp 185 medicagolgbi57. medical 1532 28 81 98.8 99.5951 blastp 21AI974377_TI 1 417 186 melonIgbI6SIA melon 1533 28 84 100 100 blastp M725511_TI nicotiana bentha nicotiana 187 mianaIgbI62|EH _bentha 1534 28 90 70.4 100 blastp 370474_TI miana 188 onionlgbI62|BE onion 1535 28 83 99.6 99.5967 blast 205571_TI 742 189 onionIgbI62|AA onion 1536 28 86 95.2 96.3414 blast 601764_TI 634 190 papayalgbI65|E papaya 1537 28 82 99.6 99.5983 blastp X255759_TI 936 191 peanutgbl61IEH peanut 1538 28 82 99.6 99.5967 blast 043676_TI 742 192 pepper 1539 28 94 86.4 100 blastp 193 periwinklelgbI6 periwink 1540 28 86 64.4 96.4071 blastp 41FD423620_TI le 856 194 U b5721B poplar 1541 28 84 100 100 blastp 195 Aob1572C poplar 1542 28 85 86.8 100 blastp 196 potatogb157.21BT potato 1543 28 81 100 100 blastp 197 radishlgbI64|EV radish 1544 28 83 94.8 94.8 blastp 531940_TI 198 radishlgbI64|EV radish 1545 28 84 92.8 95.4732 blast 527785_TI 51 199 radishlgbI64|EV radish 1546 28 84 95.2 94.8207 blastp 550763_TI 171 200 radishlgbI64|EX radish 1547 28 85 95.2 99.5815 blastp 902593_TI 9 201 radishlgbI64|EV radish 1548 28 84 96 98.7654 blast 535199_TI 321 202 radishgbI64|EV radish 1549 28 85 96 98.7654 blastp 525705_TI 321 203 safflowerlgbI621 safflower 1550 28 86 86.8 100 blastp EL399548_TI 204 safflowergbI621 safflower 1551 28 87 100 100 blastp 205_ surEL376421_ spurge 1552 28 84 391 bs 205 spurgelgbi161 ID spurge 1552 28 85 88.4 99.1031 blastp _____ V127241 TI ____ ____________ 39 ____ 45 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. strawberrygbl6 strawberry 206 41GFXDQ17802 1553 28 82 100 100 blastp 2X1T1 207 sunflowerlgbl621 sunflower 1554 28 86 100 100 blastp X95953_TI r 208 sunflowerlgbI621 sunflowe 1555 28 85 100 100 blastp DY911049_TI r 209 sunflowerlgbl621 sunflowe 1556 28 81 88.8 98.2300 blastp DY921796_TI r 885 210 sunflowerlgbl621 sunflowe 1557 28 85 100 100 blastp DY918762_TI r 211 sunflowerlgbl621 sunflowe 1558 28 81 100 100 blastp DY906198_TI r 212 sunflowerlgbl621 sunflowe 1559 28 81 100 100 blastp DY932268_TI r tobaccolgbl62|G 213 FXS45406X1_T tobacco 1560 28 93 100 100 blastp 1 214 tobacco 1621E tobacco 1561 28 89 100 100 blastp 215 apricotlgb157.21 apricot 1562 29 81 54.5454 95.8333 blastp CB818493_TI 545 333 arabidopsisgb6 arabidop 99.2094 99.6031 216 51AT4G01470_T s 1563 29 80 862 746 blastp 1 217 avocadolgbI64C avocado 1564 29 81 54.5454 93.8775 blastp K760396_TI 545 51 218 bjrapagbI62|E b rapa 1565 29 83 69.5652 94.1176 blastp X017183_TI - 174 471 219 barleylgbl57.31B barley 1566 29 81 99.2094 99.6031 blastp E413237 Ti 862 746 220 cassavalgbI64| cassava 1567 29 90 57.3122 99.3150 blast K644827_TI 53 685 221 cassavalgbI641B cassava 1568 29 82 99.2094 99.6031 blastp M259770_TI 862 746 222 cassavalgbI64| cassava 1569 29 80 99.2094 99.6031 blastp K645124_TI 862 746 223 castorbeangbI6 castorbea 1570 29 84 99.2094 99.6031 blastp 01EG666198_TI n 862 746 224 castorbeangbI6 castorbea 1571 29 82 99.2094 99.6015 blast 0IAJ605571_TI n 862 936 225 castorbeanlgbI6 castorbea 1572 29 83 99.2094 99.6031 blastp 01AJ605570_TI n 862 746 226 centaurealgbI611 centaure 1573 29 88 74.7035 97.9274 blastp EL931525_TI a 573 611 227 cichoriumlgbI61 cichoriu 1574 29 87 65.6126 99.4011 blast IEH707617_TI m 482 976 228 citrusgbl57.21C citrus 1575 29 80 94.4664 94.4444 blastp F834233_TI 032 444 229 citrusgbl57.21B citrus 1576 29 87 99.2094 99.6031 blastp Q624227_T1 862 746 230 citrusgbl57.21B citrus 1577 29 87 97.2332 98.4 blast Q623056_T1 016 231 citruslgbl57.21B citrus 1578 29 87 99.2094 99.6031 blastp Q624617_T1 862 746 46 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 232 cottonlgbl641CD cotton 1579 29 81 99.2094 99.6031 blast 486523_TI 862 746 233 cottonlgbl641AI cotton 1580 29 82 99.2094 99.6031 blast 729919_TI 862 746 234 cottongbl641BG cotton 1581 29 86 99.2094 99.6031 blast 442315_TI 862 746 235 cottonlgbl641EX cotton 1582 29 84 56.9169 100 blast 167179_TI 96 236 cottongbl641AI cotton 1583 29 82 99.2094 99.6031 blast 726375_TI 862 746 237 cowpealgbl66|F cowpea 1584 29 84 99.2094 99.6031 blastp F384697_TI 862 746 238 dandelionlgbl611 dandelio 1585 29 88 99.2094 99.6031 blast DY825779_TI n 862 746 239 fescuelgbI61ICK fescue 1586 29 80 99.2094 99.6031 blastp 802772_TI 862 746 240 grapelgbI60IBQ grape 1587 29 84 99.2094 99.6015 blast 796848_TI 862 936 241 grapelgbl601CF6 grape 1588 29 82 99.2094 99.6031 blastp 05030_TI 862 746 242 ipomoealgbI57.2 ipomoea 1589 29 85 53.7549 100 blastp IEE883704_TI iooa 18 9 8 407 10 243 ipomoealgbI57.2 ipomoea 1590 29 85 99.2094 99.6031 blastp IBJ554617_TI 862 746 244 lettucelgbI57.21 lettuce 1591 29 87 99.2094 99.6031 blast DW074608_TI 862 746 245 lettucelgb157.21 lettuce 1592 29 87 99.2094 99.6031 blastp DY977540_TI 862 746 246 lotuslgbI57.21B lotus 1593 29 80 50.1976 100 blastp W615882_TI 285 247 maizelgbl64|DQ maize 1594 29 81 99.2094 99.6031 blastp 245749_TI 862 746 248 medicagolgbl57. medicag 1595 29 82 99.2094 99.6031 blastp 21BI266516_TI 1 862 746 nicotiana bentha nicotiana 83.0039 99.5260 249 mianaIgbI62ICN _bentha 1596 29 94 526 664 blast 743053_TI miana 250 papayalgbI65|E papaya 1597 29 80 99.2094 99.6031 blastp X256526_TI 862 746 251 papayalgbI65|E papaya 1598 29 86 99.2094 99.6031 blastp X255270_TI 862 746 252 peachlgbI57.21A peach 1599 29 84 53.7549 100 blastp F367456_TI 407 253 pepperlgbI57.21 pepper 1600 29 81 73.1225 98.9304 blast CK902019_TI 296 813 254 poplarlgbl57.21A poplar 1601 29 81 99.2094 99.6031 blastp 1163470_Ti 862 746 255 poplarlgb157.21A poplar 1602 29 82 99.2094 99.6031 blastp 1166549_Ti 862 746 256 poplarlgb157.21B poplar 1603 29 81 99.2094 99.6031 blastp U887722_TI 862 746 257 poplarlgb157.21B poplar 1604 29 83 99.2094 99.6031 blastp U875073_TI 862 746 258 poplarlgbI57.21C poplar 1605 29 88 53.3596 99.2647 blastp A823737 TI 1 838 059 47 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 259 poplarlgbI57 21A poplar 1606 29 80 99.2094 99.6031 blastp 1166136_TI 862 746 260 radishlgbl64|EV radish 1607 29 80 99.2094 99.6031 blastp 544876_TI 862 746 261 ricelgbl57.21AA rice 1608 29 80 99.2094 99.6031 blast 752956_TI 862 746 262 soybeanlgbl66|C soybean 1609 29 80 99.2094 99.6031 blastp X703984_TI 862 746 263 soybeangbl66|S soybean 1610 29 86 99.2094 99.6031 blastp OYNODB_TI 862 746 264 spurgelgb6 spurge 1611 29 84 87.3517 100 blastp V146067_TI spre 11 29 8 787 10 265 spurgelgbl61 A spurge 1612 29 80 99.2094 99.6031 blastp W990927_TI 862 746 266 sunflowerlgbl621 sunflowe 1613 29 86 99.2094 99.6031 blastp DY919534_TI r 862 746 267 tobaccolgbl62|E tobacco 1614 29 92 99.2094 99.6031 blastp B443312_TI 862 746 268 tobaccolgbI621C tobacco 1615 29 81 98.0237 99.5967 blast V019217_TI 154 742 269 tobaccolgbI62|E tobacco 1616 29 92 99.2094 99.6031 blastp B443618_TI 862 746 270 tobaccolgbI62|C tobacco 1617 29 81 98.0237 99.5967 blastp V018899_TI 154 742 271 wheatIgbI641BE wheat 1618 29 80 99.2094 99.6031 blastp 418306_TI 862 746 272 wheatIgbI641BE wheat 1619 29 82 52.9644 99.2592 blastp 404792_TI 269 593 273 wheatIgbI641BE wheat 1620 29 81 99.2094 99.6031 blastp 216922_TI 862 746 274 artesia3b64 artemisia 1621 30 80 79.6 100 blastp 275 bananalgbI601ES banana 1622 30 81 88.8 93.6708 blastp 432704_TI 861 276 bananalgbI601D banana 1623 30 80 100 100 blastp N238541_TI 277 barleylgbI57' 31B barley 1624 30 80 100 100 blast E412510_TI bre 64 3 0 10 10 bat 278 cottongbl641AI cotton 1625 30 80 98.8 99.5983 blastp 726168_T1 936 279 cottogb1641AI cotton 1626 30 80 100 100 blastp 280 co0ongbi641AI cotton 1627 30 80 100 100 blastp 281 grapelg60 Q grape 1628 30 81 100 100 blastp 282 imoealgb157.2 ipomoea 1629 30 81 98 100 blastp 283 moealgbT57.2 ipomoea 1630 30 84 100 100 blastp 284 DW45 084_T1 lettuce 1631 30 80 86.8 9821981 blast 285 DWc 621_T1 lettuce 1632 30 80 88 991031 blast 48 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 286 De 5 7 778 1 lettuce 1633 30 81 50.8 100 blast DW078778_TI 287 maizegb 41C0 maize 1634 30 82 69.6 100 blastp 288 2ai32g0T4I 288 maize 1635 30 80 52.4 100 blastp 289 maizelgbl641BI6 maize 1636 30 80 54 99.2592 blast 75058_TI 593 290 W352518_Ti maize 1637 30 80 51.2 100 blastp 291 maizegbl641AF maize 1638 30 81 100 100 blastp nicotiana bentha nicotiana 292 mianaIgbl62ICN _bentha 1639 30 90 100 100 blastp 655062_TI miana nicotiana bentha nicotiana 293 mianaIgbI62ICN _bentha 1640 30 90 100 100 blastp 741621_TI miana 294 -58almgMTi oil-palm 1641 30 80 100 100 blastp 295 payalgb165E papaya 1642 30 82 100 100 blastp 296 B pepper 1643 30 91 99.2 98.8 blastp 297 periwinklelgbI6 periwink 1644 30 82 100 100 blast 41EG554262_TI le 298 p n petunia 1645 30 89 100 100 blastp 299 potatolgbl57.21C potato 1646 30 96 92 91.3043 blastp K853059_TI 478 300 potato gb157.21C potato 1647 30 82 60.4 100 blast K852742_TI poao 1430 8 604 10 bat 301 potatolgb157.21B potato 1648 30 99 81.2 97.5961 blastp M407759_TI 538 302 potatolgb157.21C potato 1649 30 98 65.2 92.0903 blastp K718033_TI 955 303 potatolgb157.21C potato 1650 30 100 62 95.0920 blastp K717899_TI 245 304 potatogbl57.21C potato 1651 30 97 79.6 95.2153 blastp V472240_TI 11 305 potatolgb157.21B potato 1652 30 95 93.2 99.5726 blastp G098199_TI 496 306 ricelgbl57IU37 rice 1653 30 81 100 100 blastp 307 ryel426_1E49 rye 1654 30 80 100 100 blastp sorghumgbl61. 308 xenolAF037061_ sorghum 1655 30 80 100 100 blastp TI sugarcanelgbI57 can 309 .21BQ535365 T sugar 1656 30 80 80.4 100 blastp 1 310 switchgrasslgbI6 switchgr 1657 30 81 100 100 blastp 51DN141449_TI ass I III _I _ I 49 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 311 switchgrasslgbl6 switchgr 1658 30 81 100 100 blastp 51DN142089_TI ass 312 tobaccolgb62lC tobacco 1659 30 90 100 100 blastp N824866_TI 313 tobaccolgb1621lC tobacco 1660 30 90 100 100 blastp V017118_TI 314 wheatlgb1641TA wheat 1661 30 80 100 100 blastp U86762_TI 315 wheat~gb1641BE wheat 1662 30 80 72 100 blastp 499589_TI 316 DWc 170_T1 lettuce 1663 31 80 100 100 blastp 317 tobaccolgbl621E tobacco 1664 32 87 50.7692 85.1612 blastp H616288_TI 308 903 318 applelgb157.31C apple 1665 33 86 98.2638 98.9510 blastp 0068608_TI 889 49 319 applelgb157.31A apple 1666 33 83 98.2638 99.3079 blast B100869_TI 889 585 320 applelgb157.31A apple 1667 33 83 98.2638 99.3079 blastp B100870_TI 889 585 321 applelgb157.31C apple 1668 33 86 54.8611 90.2857 blastp N860225_TI 111 143 322 applelgb157.31C apple 1669 33 85 98.2638 98.9510 blastp K900645_TI 889 49 323 apricotlgb157.21 apricot 1670 33 89 51.0416 98.6577 blastp CB822297_TI 667 181 324 apricotlgb157.21 apricot 1671 33 83 98.2638 98.9655 blastp CB819647_TI 889 172 325 aquilegialgbl57. aquilegia 1672 33 86 98.2638 98.9547 blastp 31DR917005 Ti 889 038 arabidopsislgb 16 arabio 73.1astp.60 326 51AT4G0430T idop 1673 33 84 73.6111 97.2602 blast arabidopsislgb16 arabidop 88.1944 84.3853 327 51AT2G45960_T s 1674 33 86 444 821 blastp 3 arabidopsislgb16 arabidop 98.2638 98.9547 328 51AT4G23400_T s 1675 33 87 889 038 blastp 1 arabidopsislgb16 arabidop 98.2638 98.9510 329 51AT2G45960T i 1676 33 86 889 49 blastp 1 arabidopsislgb16 arabidop 98.2638 98.9547 330 51AT4G00430_T s 1677 33 87 889 038 blastp 1 arabidopsislgb16 arabidop 98.2638 98.9510 331 51ATiGs|620T aid 1678 33 88 889 49 blastp 4 arabidopsislgb16 arabidop 98.2638 98.9510 332 51AT3G61430_T 1679 33 85 889 49 blast arabidopsislgb 16 arabidop 88.1944 92.7007 333 51AT2G45960_T s 1680 33 86 44 299 blastp 4Si 34 artemisialgbI64l reii 18 3 8 98.2638 98.9547 blat EY046087 TI atmsa 18 3 8 889 038 50 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 335 artemisialgbI641 artemisia 1682 33 87 73.6111 99.0697 blastp EY046310_TI 111 674 336 artemisialgbl641 artemisia 1683 33 84 97.5694 98.2758 blastp EY032836_TI 444 621 337 artemisialgbl641 artemisia 1684 33 84 89.2361 99.2307 blast EY031810_TI 111 692 338 avocadolgbl641C avocado 1685 33 86 98.2638 98.9547 blastp K751385_TI 889 038 339 avocadolgbl641C avocado 1686 33 91 73.6111 99.0654 blastp K745633_TI 111 206 b-juncealgbl641 98.2638 98.9510 340 EVGN00081008 b-juncea 1687 33 87 889 49 blastp 450640_TI b-juncealgbl641 60.4166 96.1325 341 EVGN00515811 b-juncea 1688 33 90 667 967 blastp 862066_TI b-juncealgbl641 73.6111 99.0654 342 EVGN00230716 b-juncea 1689 33 89 111 206 blastp 760965_TI b-juncealgbl641 66.3194 96.4646 343 EVGN01776308 b-juncea 1690 33 84 444 465 blastp 261252_TI b-juncealgbl641 65.2777 98.9473 344 EVGN00910030 b-juncea 1691 33 91 778 684 blastp 360678_TI b-juncealgbl641 51.0416 98.6577 345 EVGN03812526 b-juncea 1692 33 88 667 181 blastp 911787_TI b-juncealgbl641 65.2777 98.9473 346 EVGN00227203 b-juncea 1693 33 91 778 684 blastp 510305_TI b-juncealgbl641 98.2638 98.9510 347 EVGN00462518 b-juncea 1694 33 87 889 49 blastp 410866_TI b-juncealgbl641 73.2638 99.0610 348 EVGN00248411 b-juncea 1695 33 89 889 329 blastp 120906_TI 349 b-juncealgbl641 bjuncea 1696 33 86 98.2638 98.2638 blast EF471211_TI 889 889 b-juncealgbl641 98.2638 98.9510 350 EVGN00440012 b-juncea 1697 33 86 889 49 blastp 650683_TI b-juncealgbl641 98.2638 98.9510 351 EVGN00452211 b-juncea 1698 33 86 889 49 blastp 183349_TI b-juncealgbl641 57.6388 93.2584 352 EVGN03595331 b-juncea 1699 33 89 889 27 blastp 210044_TI b-juncealgbl641 98.2638 98.9510 353 EVGN00088009 b-juncea 1700 33 87 889 49 blastp 631302_TI b-juncealgbl641 51.7361 93.9075 354 EVGN00512912 b-juncea 1701 33 85 111 63 tblastn 541009_TI 51 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. bjuncealgb1641 69.0972 90.3177 355 EVGN00756014 b-juncea 1702 33 84 222 005 tblastn 550623_TI 356 boleracealgbl6 bolerac 1703 33 86 98.2638 98.9510 blastp 1AF299051_TI ea 889 49 357 boleracealgbl6 bolerac 1704 33 87 75.3472 99.5412 blastp 1|AM391520_TI ea 222 844 358 boleracealgbl6 bolerac 1705 33 87 98.2638 98.9510 blastp 1AM058918_TI ea 889 49 359 boleracealgbl6 bolerac 1706 33 86 98.2638 98.9510 blastp 1|AF299050_TI ea 889 49 360 boleracealgbl6 bolerac 1707 33 86 98.2638 98.9510 blast 1DY029936_TI ea 889 49 361 boleracealgbl6 bolerac 1708 33 87 78.4722 100 blastp 1EH422530_TI ea 222 362 b-rapalgbl621C b rapa 1709 33 84 71.5277 99.5169 blastp V546930_TI - 778 082 363 b-rapalgbl621B b rapa 1710 33 86 95.4861 99.2779 blastp G544387_TI - 111 783 364 b-rapagbl62|C b rapa 1711 33 86 98.2638 98.9510 blastp A992432_TI - 889 49 365 b-rapalgbl621C b rapa 1712 33 83 54.8611 99.3710 blastp V546129_T2 - 111 692 366 b-rapalgb1621EE brapa 1713 33 87 98.2638 98.9510 blast 526280_TI - 889 49 367 b-rapalgb1621L3 b rapa 1714 33 86 98.2638 98.9510 blastp 3552_TI 889 49 368 b-rapalgbl621B b rapa 1715 33 84 77.0833 99.5515 blastp G543719_TI - 333 695 369 b-rapalgbl621AF b rapa 1716 33 87 98.2638 98.9510 blastp 004293_TI - 889 49 370 b-rapalgbl621B brapa 1717 33 87 98.2638 98.9510 blast G544086_TI - 889 49 371 b-rapalgbl621C b rapa 1718 33 86 98.2638 99.2982 blastp X267412_TI - 889 456 372 b-rapalgbl621C b rapa 1719 33 86 98.2638 98.2638 blastp V546129_TI - 889 889 373 b-rapalgbl621C b rapa 1720 33 83 56.5972 90.5555 blastp V545634_TI - 222 556 374 bananalgbl601D banana 1721 33 84 60.7638 99.4285 blastp N238827_TI 889 714 375 bananalgbl601ES banana 1722 33 89 63.8888 98.9247 blastp 431094_TI 889 312 376 barleylgb157.31B barley 1723 33 83 98.2638 98.9726 blast E412959_T2 889 027 b 377 barleylgb157.31A barley 1724 33 85 99.3055 99.6551 blastp L507831_TI 556 724 378 barleylgb157.31B barley 1725 33 83 98.2638 98.9726 blastp E412959_Ti 889 027 379 barleylgb157.31B barley 1726 33 82 98.2638 98.9830 blastp E412959_T5 889 508 380 barleylgb157.31B barley 1727 33 82 98.2638 98.9830 blastp E412959_T4 889 508 381 barleylgb157.31A barley 1728 33 82 98.2638 98.9726 blastp L502020 TI 889 027 52 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 382 barleylgb157. 31B barley 1729 33 85 98.2638 98.9583 blastp E412972_TI 889 333 383 basilicumlgbl57. basilicu 1730 33 88 61.8055 99.4413 blastp 31DY340092_TI m 556 408 384 basilicumlgbl57. basilicu 1731 33 87 73.9583 99.5327 blast 31DY332264_TI m 333 103 385 beanlgb1641CB5 bean 1732 33 88 98.2638 98.9547 blastp 43592_TI 889 038 386 beanlgbl641PVU bean 1733 33 84 98.2638 99.3079 blastp 97023_TI 889 585 387 beanlgb1641CB5 bean 1734 33 84 98.6111 99.6539 blast 42193_TI 111 792 388 beetlgbl621BVU beet 1735 33 84 98.2638 98.9510 blastp 60149_TI 889 49 brachypodiumig brachypo 82.9861 95.6521 389 b16I.xeno1BE44 dim 1736 33 83 11 79 blastp 3278_TI brachypodiumig brachypo 98.2638 98.9583 390 b16I.xeno1BE2I du 1737 33 85 98268 98.9583 s 6990_TI brachypodiumig brachypo 86.8055 72.7011 391 b16I.xeno1BE40O iu 1738 33 82 56 44 blastp 3307_TI 392 canolalgbl61|CN canola 1739 33 86 98.2638 98.9510 blastp 731957_TI 889 49 393 canolagbIICX anola 1740 33 86 98.2638 98.9510 blastp 194503_TI 889 49 394 canola~gbl6I1CD canola 1741 33 87 98.2638 98.9510 blastp 814405_TI 889 49 395 canolalgbl61IEG canola 1742 33 86 98.2638 98.9510 blastp 020906_TI 889 49 396 canolalgb1611H7 canola 1743 33 86 98.2638 98.9510 blast 4720_TI 889 49 397 canolalgbl61|CN canola 1744 33 86 84.0277 93.4362 blastp 831315_TI 778 934 398 canolalgbl6I1CD canola 1745 33 87 98.2638 98.9510 blastp 817408_TI 889 49 399 canolalgb1611EE canola 1746 33 89 52.7777 98.7096 blast 502121_TI 778 774 400 canolagbl61|CX canola 1747 33 87 98.2638 98.9510 blastp 187544_TI 889 49 401 canola~gbl6I1CD canola 1748 33 86 98.2638 98.9510 blastp 822064_TI 889 49 402 canola~gbl6I1CD canola 1749 33 81 92.0138 93.0313 blastp 824965_TI 889 589 403 canolalgbl61|EE canola 1750 33 87 98.2638 98.9510 blast 485551_TI 889 49 404 canolalgbl61ICB canola 1751 33 86 98.2638 98.9510 blastp 686274_TI 889 49 405 canolalgbl6I1CD canola 1752 33 83 76.3888 94.0425 blastp 814573_TI 889 532 406 canola~gbl6I1CX canola 1753 33 86 98.2638 98.2638 blastp 193398_TI 889 889 407 canola~gbl6I1CD canola 1754 33 86 98.2638 98.9510 blastp 818853_TI c 889 49 53 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 408 canolalgbl61|D canola 1755 33 85 78.4722 99.5594 blastp Y005979_TI 222 714 409 canolalgbl61IEE canola 1756 33 85 81.5972 99.5762 blastp 464964_TI 222 712 410 cassavalgbl641B cassava 1757 33 85 97.9166 99.3031 blast M260264_TI 667 359 411 cassavalgbl64| cassava 1758 33 87 73.6111 99.0697 blastp K901165_TI 111 674 412 cassavalgbl64| cassava 1759 33 87 98.2638 98.9547 blastp K642415_TI 889 038 413 cassavalgbl64|D cassava 1760 33 85 97.9166 99.3031 blastp V455398_TI 667 359 414 castorbeanlgbl6 castorbea 1761 33 89 98.2638 98.9547 blast 01T14819_TI n 889 038 415 castorbeanlgbl6 castorbea 1762 33 86 98.2638 98.9510 blastp 01EG691229_TI n 889 49 416 castorbeanlgbl6 castorbea 1763 33 87 97.9166 99.3031 blast 01AJ605566_TI n 667 359 417 castorbeanlgbi6 castorbea 98.2638 99.3055 blast 41 1OMDL29969M0 1764 33 84 889 556 bat 00266_TI 418 castorbeanlgb16 castorbea 1765 33 87 97.9166 98.9583 blastp 01AJ605574_TI n 667 333 419 centaurealgbl6l1 centaure 1766 33 82 97.5694 98.6062 blastp EH732068_TI a 444 718 420 cichoriumlgbl61 cichoriu 1767 33 87 98.2638 98.9547 blast IEH673032_TI m 889 038 421 cichoriumlgbl61 cichoriu 1768 33 90 51.7361 98.6842 blastp IEH706808_TI m 111 105 422 cichoriumlgbl61 cichoriu 1769 33 86 64.9305 95.4314 blastp IEH701938_TI m 556 721 423 citrusgbl57.21C citrus 1770 33 82 69.4444 99.5098 blast 0912471_TI 444 039 424 citruslgbl57.21B citrus 1771 33 88 98.2638 98.9547 blastp Q624312_T1 889 038 425 citruslgb157.21C citrus 1772 33 84 92.7083 94.0559 blastp N182376_TI 333 441 426 citrusgbl57.21C citrus 1773 33 89 98.2638 98.9547 blastp B291370_TI 889 038 427 citrusgbI57.21C citrus 1774 33 85 98.2638 99.3031 blast F833327_TI 889 359 428 citrusgbl57.21B citrus 1775 33 85 97.9166 99.6503 blast Q624860_T1 667 497 429 citrusgbl57.21C citrus 1776 33 81 97.9166 99.6503 blastp F508404_TI 667 497 430 citrusgbl57.21C citrus 1777 33 84 98.2638 99.3031 blast B293694_TI 889 359 431 citruslgbl57.21B citrus 1778 33 85 97.9166 99.6503 blastp Q622975_T1 667 497 432 citruslgbl57.21B citrus 1779 33 86 73.6111 99.5305 blastp E213453_TI 111 164 433 citrusgbl57.21C citrus 1780 33 85 98.6111 99.6527 blastp F828110_TI 111 778 434 citruslgbl57.21B citrus 1781 33 86 93.4027 99.6336 blastp Q623397T1 778 996 54 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 435 cloverlgbl621BB clover 1782 33 85 98.6111 99.6539 blastp 903117_TI 111 792 436 coffealgbl57.21B coffee 1783 33 88 98.9583 99.3055 blast Q449035_T1 333 556 437 coffealgbl57.21D coffee 1784 33 85 98.2638 98.9473 blast V663743_TI 889 684 438 cottongb1641CD cotton 1785 33 83 98.2638 99.3055 blast 486529_TI 889 556 439 cottonlgbl1641BE cotton 1786 33 87 98.2638 98.9547 blast 052445T1 889 038 440 cottongb1641AI cotton 1787 33 86 98.2638 98.9547 blast 726690_TI 889 038 441 cottongb1641DN cotton 1788 33 83 87.8472 98.8281 blast 803576_TI 222 25 442 cottongb1641B cotton 1789 33 81 98.2638 99.2882 blast M358242_TI 889 562 443 cottongb1641C0 cotton 1790 33 81 52.7777 99.3506 blast 085369_TI 778 494 444 cottongb1641C0 cotton 1791 33 84 98.2638 99.3055 blast 098674_TI 889 556 445 cottonlgb1641C0 cotton 1792 33 84 98.2638 99.3031 blast 070796_TI 889 359 446 cottongb1641AI cotton 1793 33 85 98.2638 99.3079 blast 729945_TI 889 585 447 cottongb1641D cotton 1794 33 86 80.5555 98.3122 blast W496760_TI 556 363 448 cowpealgb1661F cowpea 1795 33 82 98.2638 99.3031 blastp F384916_TI 889 359 449 cowpealgb1661F cowpea 1796 33 82 98.6111 99.6539 blast F555791_TI 111 792 450 cowpealgbl661F cowpea 1797 33 87 98.2638 98.9547 blastp C457489_TI 889 038 451 cowpealgbl661A cowpea 1798 33 84 98.2638 99.3079 blastp B037241_TI 889 585 cryptomerialgbl rpoe7.57 951 452 661DC429824T rme 1799 33 84 71.5277 99.5215 blastp 1 n cryptomerialgbl rpoe9.61 961 453 661AU036730T cryp 1800 33 85 98.6111 996515 blast 1 454 dandelionlgbI611 dandelio 1801 33 84 82.2916 93.7007 blastp DY814032_TI n 667 874 455 dandelionlgbI611 dandelio 1802 33 82 98.2638 99.3031 blastp DY802714_TI n 889 359 456 dandelionlgbI611 dandelio 1803 33 84 95.8333 99.6415 blastp DY822683_TI n 333 771 457 dandelionlgbI611 dandelio 1804 33 87 98.2638 98.9583 blastp DY806788_TI n 889 333 458 dandelionlgbI611 dandelio 1805 33 84 84.0277 99.5918 blastp DY808781_TI n 778 367 459 dandelionlgbI611 dandelio 1806 33 81 76.3888 94.8497 blastp DY810613_TI n 889 854 460 fescuelgbI61ICK fescue 1807 33 85 97.9166 98.6111 blastp 803261 Ti 667 111 55 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 461 fescuelgbI61IDT fescue 1808 33 84 67.3611 99.4923 blastp 682664_TI 111 858 462 fescuelgbI61IDT fescue 1809 33 83 98.2638 98.9655 blastp 677062_TI 889 172 463 fescuelgbl61|DT fescue 1810 33 86 98.2638 98.9619 blast 679061_TI 889 377 464 flaxlgb157.31CV flax 1811 33 84 71.875 99.5260 blastp 478314_TI 664 465 gingerlgbl641DY ginger 1812 33 88 98.2638 98.9510 blastp 345344_TI 889 49 466 gingerlgbl641DY ginger 1813 33 85 98.2638 98.9473 blastp 358322_TI 889 684 467 gingerlgbl641DY ginger 1814 33 84 98.6111 99.6478 blastp 360757_TI 111 873 468 gingerlgbl641DY ginger 1815 33 86 98.2638 98.9473 blastp 345596_TI 889 684 469 grapegbrape 1816 33 87 98.2638 98.9547 blast 88844_TI 889 038 470 grapelgbl601AF1 grape 1817 33 85 98.2638 98.9510 blastp 88843_TI 889 49 471 grapelgbl601AF1 grape 1818 33 85 98.2638 98.9510 blastp 88843_T3 889 49 472 grapelgbl601CB grape 1819 33 87 98.2638 99.3006 blastp 971128_TI 889 993 473 grapegb6AF rape 1820 33 84 72.2222 86.3070 blast 88843_T4 222 539 474 iceplantgb1641 iceplant 1821 33 85 98.2638 98.9473 blast MCU26537 I 889 684 475 iceplantlgb1641C iceplant 1822 33 85 98.2638 99.2957 blastp IPMIPA_Ti 889 746 476 iceplantgb1641C iceplant 1823 33 87 98.2638 98.9473 blastp IPMIPB_Ti 889 684 477 ipomoealgbl57.2 ipomoea 1824 33 87 98.9583 99.3031 blastp IBM878883_TI 333 359 478 ipomoealgbl57.2 ipomoea 1825 33 85 98.6111 99.6491 blastp IBJ553988_TI 111 228 479 ipomoealgbl57.2 ipomoea 1826 33 84 98.6111 99.6478 blast IBJ553369_T1 111 873 480 ipomoealgbl57.2 ipomoea 1827 33 89 98.9583 99.3031 blastp IBJ553198_TI 333 359 481 lettucelgbl57 .21 lettuce 1828 33 86 98.2638 97.9310 blastp DW079915_TI 889 345 482 lettucelgbl57.21 lettuce 1829 33 87 98.2638 98.9547 blastp DW043941_TI 889 038 483 Dtcg538_T1 lettuce 1830 33 87 96.875 98.2456 blastp 484 lettucelgbl57 .21 lettuce 1831 33 84 98.2638 99.3031 blastp DW104582_TI 889 359 485 lettucelgb157.21 lettuce 1832 33 84 98.2638 99.3031 blastp DWO44606_TI 889 359 486 lettucelgbl57.21 lettuce 1833 33 85 89.2361 94.1605 blastp DW148209_TI 111 839 487 lettucelgbl57.21 lettuce 1834 33 83 98.6111 95.3333 blast DW148478 TI 111 333 56 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 488 lettucelgbl57 .21 lettuce 1835 33 86 98.2638 98.9547 blastp DW108503_TI 889 038 489 lettucegb57 21 lettuce 1836 33 86 96.875 99.6441 blastp DW046100_TI lete 281 490 lettucelgbl57.21 lettuce 1837 33 85 98.2638 99.3031 blast DW075079_TI 889 359 491 lettucelgbl57 .21 lettuce 1838 33 87 98.2638 98.9547 blastp DW076402_TI 889 038 492 lettucelgbl57.21 lettuce 1839 33 86 89.5833 92.8315 blastp DW084041_TI 333 412 493 lettucelgbl57.21 lettuce 1840 33 87 98.2638 98.9547 blastp DW145601_TI 889 038 494 lettucelgbl57.21 lettuce 1841 33 86 98.2638 98.9547 blastp CV699980_TI 889 038 495 lettucelgb157.21 lettuce 1842 33 87 98.2638 98.9547 blastp DW064849_TI 889 038 496 lettucelgbl57.21 lettuce 1843 33 83 98.6111 89.0965 blast DW147179_TI 111 732 497 lettucelgbl57.21 lettuce 1844 33 83 98.6111 97.2789 blastp DW045991_TI 111 116 498 lotuslgbl57.21AF lotus 1845 33 84 98.2638 99.3079 blastp 145707_Ti 889 585 499 lotuslgbl57.21AI lotus 1846 33 86 96.875 98.2456 blastp 967594_TI 14 500 lotuslgb157.21AF lotus 1847 33 87 66.6666 100 blastp 145708_Ti 667 501 maize1gb1641EC maize 1848 33 86 54.5138 98.7421 blastp 881658_TI 889 384 502 maizelgb1641AI3 maize 1849 33 85 98.2638 98.9583 blastp 72377_TI 889 333 503 maizelgbl641AF maize 1850 33 83 60.7638 100 blast 145706_TI 889 504 maizelgbl641AI8 maize 1851 33 84 98.2638 98.9726 blastp 55222_TI 889 027 505 maizelgb1641AI6 maize 1852 33 86 98.2638 98.9619 blastp 19392_TI 889 377 506 maizelgb1641AI8 maize 1853 33 84 98.2638 98.9583 blastp 61086_TI 889 333 medicagolgb 157. medical 823 937 507 21AW684000T cag 1854 33 84 982638 99.3079 blast 508 medicagolgbl57. medicag 1855 33 83 98.2638 99.3079 blastp 21AI974398_TI 1 889 585 509 medicagolgb157. medicag 1856 33 88 97.5694 98.6062 blastp 21AL366983_TI 1 444 718 510 medicagolgbl57. medicag 1857 33 84 98.6111 99.3103 blastp 21AI737528_TI 1 111 448 511 medicagolgbl57. medicag 1858 33 84 82.2916 87.2262 blastp 21BQ151876_TI 1 667 774 512 melonlgbl651DV melon 1859 33 84 98.2638 99.3150 blastp 632745_TI 889 685 513 melonlgbl651CF melon 1860 33 83 98.2638 99.3150 blastp 674915_TI 889 685 514 melon~gbl651DV melon 1861 33 85 98.2638 98.9510 blastp 632772 TI 889 49 57 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 515 milletlgbl6I1CD millet 1862 33 83 57.2916 92.1787 blastp 724341_TI 667 709 nicotiana bentha nicotiana 66.6666 97.9487 516 mianalgbl621ES _bentha 1863 33 84 667 179 blast 885295_TI miana 517 oil-palmlgbl661 oil palm 1864 33 85 98.2638 98.9547 blastp CN600863_TI 889 038 518 oil-palmlgbl661 oil palm 1865 33 86 98.2638 98.9547 blastp CN600797_TI 889 038 519 onionlgbl621AF onion 1866 33 86 98.2638 98.9583 blastp 255796_TI 889 333 520 papayalgbl651E papaya 1867 33 84 72.2222 93.2735 blastp X228092_TI 222 426 521 papayalgbl651AJ papaya 1868 33 85 98.2638 99.3079 blastp 000031_TI 889 585 522 papayalgbl651E papaya 1869 33 83 98.2638 99.3031 blastp X257869_TI 889 359 523 papayalgbl651A papaya 1870 33 90 98.2638 98.9510 blastp M903842_TI 889 49 524 peachlgbl57.21B peach 1871 33 84 98.2638 98.9655 blastp U039203_TI 889 172 525 peachlgbl57.21B peach 1872 33 90 51.3888 98.6666 blastp U040913_TI 889 667 526 peanutlgbl61 IC peanut 1873 33 83 98.6111 99.6539 blast D038184_TI 111 792 527 peanutlgb 161 1ES peanut 1874 33 82 73.9583 99.5348 blastp 490696_TI 333 837 528 peanutlgb61| C peanut 1875 33 83 98.2638 99.3079 blastp D038104_TI 889 585 529 pepper 1876 33 97 96.875 100 blastp 530 pepperlgb157.21 pepper 1877 33 95 99.3055 100 blastp BM063708_TI ppe 87 3 556 10 531 periwinklelgbl6 periwink 1878 33 88 98.9583 99.3031 blastp 41EG554502_TI le 333 359 532 periwinklelgbl6 periwink 1879 33 87 98.9583 99.3031 blastp 41EG554518_TI le 333 359 533 periwinklelgbl6 periwink 1880 33 83 84.0277 96.8 blast 41EG556773_TI le 778 534 petunialgbl57.21 petunia 1881 33 93 99.3055 100 blastp AF452010_TI 556 535 petunialgb57.21 petunia 1882 33 92 61.8055 100 blastp CV292775_TI ptna 18 3 9 556 10 536 petunialgb157.21 petunia 1883 33 86 98.2638 99.3006 blastp AF452011_TI 889 993 537 pinelgb157.21AL pine 1884 33 83 98.2638 98.9583 blast 751335_TI 889 333 538 pinelgb157.21AA pine 1885 33 85 98.2638 98.9583 blastp 556193_TI 889 333 539 pinelgb157.21AL pine 1886 33 85 98.2638 98.9583 blastp 750485_TI 889 333 540 pineapplelgbl57. pineappi 1887 33 83 98.2638 97.9452 blast 21DT335964_TI e 889 055 541 pineapplelgbl57. pineapple 1888 33 86 98.2638 98.9583 blast 21DT338557 TI e 889 333 58 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 542 poplarlgb157 21B poplar 1889 33 83 98.2638 99.3031 blastp U817536_TI 889 359 543 poplarlgbl57 21A poplar 1890 33 88 98.2638 98.9583 blastp 1162483_TI 889 333 544 poplarlgbl57.21B poplar 1891 33 87 97.9166 99.3031 blast 1122420_Ti 667 359 545 poplarlgb157.21A poplar 1892 33 85 97.9166 99.3031 blastp 1165418_Ti 667 359 546 poplarlgb157.21B poplar 1893 33 82 88.1944 92.0863 blastp U817536_T3 444 309 547 poplarlgb157.21B poplar 1894 33 83 98.2638 99.3031 blastp U881784_TI 889 359 548 potatolgbl57.21B potato 1895 33 85 98.2638 99.3006 blastp E923816_TI 889 993 549 potatogbl57.21C potato 1896 33 91 62.5 98.9010 blastp K260061_TI 989 550 potatolgb157.21B potato 1897 33 86 98.2638 98.9473 blast E924585_TI 889 684 551 potatolgbl57.21B potato 1898 33 86 61.1111 100 blastp F153976_TI poaoI88I3 8 552 potatogbl5721A potato 1899 33 97 100 100 blastp 553 potatolgb157.21B potato 1900 33 92 99.3055 100 blastp F154021_TI 556 554 potatolgbl57.21B potato 1901 33 95 98.9583 99.3031 blast G599633_TI 333 359 555 potatolgb157.21B potato 1902 33 85 98.2638 99.3006 blastp E922307_TI 889 993 556 radishlgbl641EV radish 1903 33 86 98.2638 98.9510 blastp 536875_TI 889 49 557 radishlgbl641EW radish 1904 33 83 60.0694 96.1111 blastp 726189_TI 444 111 558 radishlgbl641EX radish 1905 33 86 98.2638 98.9510 blastp 756217_TI 889 49 559 radishlgbl641AB radish 1906 33 86 98.2638 98.9510 blastp 030696_TI 889 49 560 radishlgbl641AB radish 1907 33 87 98.2638 98.9510 blastp 030695_TI 889 49 561 radishgbl641AB radish 1908 33 85 98.2638 98.9510 blast 012044_TI 889 49 562 radishlgbl641EV radish 1909 33 87 98.2638 98.9547 blastp 567230_TI 889 038 563 radishlgbl641EY radish 1910 33 86 98.2638 98.9510 blastp 936735_TI 889 49 564 ricelgb157.21U37 rice 1911 33 86 98.2638 98.9619 blast 951_TI 889 377 565 ricelgb157.21U40 rice 1912 33 86 98.2638 98.9583 blastp 140_TI 889 333 566 ricelgb157.21BE0 rice 1913 33 82 98.2638 98.9583 blastp 39992_TI 889 333 567 ricelgb157.21U37 rice 1914 33 86 73.6111 99.0654 blastp 951_T2 111 206 568 roselgb157.21BQ rose 1915 33 83 97.5694 98.6206 blastp 104887 TI 444 897 59 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 569 roselgb157.21BQ rose 1916 33 85 98.2638 98.9547 blastp 103877_TI 889 038 570 roselgb157.21EC rose 1917 33 80 62.1527 99.4444 blastp 586734_TI 778 444 571 ryelgbl641BE58 rye 1918 33 83 98.2638 98.9726 blast 6240_TI 889 027 572 safflowerlgbl621 safflower 1919 33 86 89.5833 94.5454 blastp EL407054_TI 333 545 573 safflowerlgbl621 safflower 1920 33 85 98.2638 92.5081 blastp EL400504_TI 889 433 574 safowerlgbl621 safflower 1921 33 83 84.375 99.5934 blastp EL400004_TI 959 575 sesamelgbl57.21 sesame 1922 33 91 57.2916 100 blastp BU668587_TI seae 1233 9 667 576 sesamelgbl57.21 sesame 1923 33 87 55.2083 99.375 blastp 56 BU669929_TI seae39333 8 sorghumlgbl61. 98.2638 98.9583 577 xenoIAI372377_ sorghum 1924 33 85 889 333 blastp TI sorghumgbl61. 98.2638 98.9655 578 xenolA1861086_ sorghum 1925 33 82 889 172 blastp T1 sorghumgbl61. 98.2638 98.9619 579 xenolSBU87981 sorghum 1926 33 86 889 377 blastp TI 580 soybeanlgbl661C soybean 1927 33 82 98.2638 99.3031 blastp D401115_TI 889 359 581 soybeanlgbl661A soybean 1928 33 84 98.6111 99.6539 blastp W348556_TI 111 792 582 soybeanlgbl661B soybean 1929 33 86 98.2638 98.9547 blastp E352670 TI 889 038 583 soybeanlgbl661B soybean 1930 33 86 98.2638 98.9619 blast 1967765_TI 889 377 584 soybeanlgbl661B soybean 1931 33 82 98.2638 99.3079 blastp E661219_TI 889 585 585 soybeanlgbl661B soybean 1932 33 81 88.1944 98.4732 blastp E352747_T5 444 824 586 soybeanlgbl661C soybean 1933 33 85 97.5694 98.6013 blastp D416359_TI 444 986 587 soybeanlgbl661A soybean 1934 33 84 97.5694 98.6013 blastp W350352_TI 444 986 588 soybeanlgbl661B soybean 1935 33 82 98.2638 99.3079 blastp E352747_TI 889 585 589 soybeanlgbl661B soybean 1936 33 85 98.2638 99.2957 blastp E820629_TI 889 746 590 spikemosslgbl65 spikemos 1937 33 82 51.0416 99.3243 blast IDN838148_T4 1 667 243 591 sprucelgbl62C0 spruce 1938 33 80 96.875 98.9473 blastp 592 sprucelgbl621CO spruce 1939 33 86 98.2638 98.9726 blastp 216100_TI 889 027 593 sprucelgbl621CO spruce 1940 33 85 98.2638 98.9583 blastp 216028_TI 889 333 594 spurgelgbl61| B spurge 1941 33 87 97.9166 98.2638 blast G354070 TI 667 889 60 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 595 strawberrylgbl6 strawberry 1942 33 82 98.2638 99.3103 blastp 41CO378647_T1 y 889 448 596 strawberrylgbl6 strawberry 1943 33 84 98.2638 98.9547 blastp 41CX661400T1 y 889 038 597 strawberrylgbl6 strawberry 1944 33 83 98.2638 98.9510 blast 41DV438296T1 y 889 49 598 sugarcanelgb57 1can 60.0694 88.8888 blast 58 .21CA264801 T sua 1945 33 80 44 889 bat 1 sugarcanelgb157 suacan9823 9.53 599 .21CA086058 T sugar 1946 33 85 982638 98.9583 blast 1 600 sugarcanelgb57 1can 97.2222 98.2638 blast 60 .21CA071197_T sua 1947 33 84 222 889 bat 1 601 sugarcanelgb57 1can 91.6666 99.2537 blast 61 .21BQ530399_T sua 1948 33 84 667 313 bat 1 602 sugarcanelgb57 1can 74.3055 99.5391 blast 62 .21CA085969_T sua 1949 33 82 556 705 bat 1 603 sugarcanelgb57 sugarcan 71.1805 93.6936 blast 63 .21CA074778_T sua 1950 33 84 556 937 bat 1 604 sugarcanelgb57 1can 62.1527 99.4505 blast 64 .21CA130651_-T sua 1951 33 81 778 495 bat 1 605 sugarcanelgb57 1can 53.4722 98.7179 blast 65 .21AA525652_T sua 1952 33 88 222 487 bat 1 sugarcanel1,b 15 7 sgr 823 891 606 .21BQ536359T sugarcane 1953 33 86 98.2638 98.9619 blastp 1 607 sunflowerlgb1621 sunflowe 1954 33 86 98.2638 98.9547 blastp DY909123_TI r 889 038 608 sunflowerlgb1621 sunflowe 1955 33 85 98.2638 98.9547 blastp CD846367_TI r 889 038 609 sunflowerlgb1621 sunflowe 1956 33 86 98.2638 98.9547 blastp CD846084_TI r 889 038 610 sunflowerlgbl621 sunflowe 1957 33 84 72.2222 100 blastp DY915760_TI r 222 611 sunflowerlgbl621 sunflowe 1958 33 83 73.6111 99.5327 blastp CF080940_TI r 111 103 612 sunflowerlgbl621 sunflowe 1959 33 83 96.875 97.5694 blast CF087907_TI r 444 613 sunflowerlgbl621 sunflowe 1960 33 84 98.2638 98.6111 blast CX946986_TI r 889 111 614 sunflowerlgb1621 sunflowe 1961 33 87 73.6111 99.0697 blastp DY918780_TI r 111 674 615 switchgrasslgbl6 switchgr 1962 33 86 98.2638 98.9583 blastp 51FE619753_TI ass 889 333 616 switchgrasslgbl6 switchgr 1963 33 85 98.2638 98.9583 blastp 51DN142591_TI ass 889 333 617 switchgrasslgbl6 switchgr 1964 33 85 98.2638 98.9619 blastp 51DN141716 T1 ass 889 377 61 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 618 switchgrasslgbl6 switchgr 1965 33 86 98.2638 98.9583 blastp 51DN141343_TI ass 889 333 619 switchgrasslgbl6 switchgr 1966 33 85 98.2638 98.9619 blastp 51DN142037_TI ass 889 377 thellungiellalgbi thellungi 1967 33 85 98.2638 98.9510 blast 620 57.21DN774595 ella 197 3 5 889 49 bat TI thellungiellalgb thellungi 98.2638 98.9510 621 57.21BM986095 ela 1968 33 86 88 9 blastp -T1 thellungiellalgbl theungi 72.5694 99.5238 622 57.21BI698563 tellung 1969 33 85 44 05 bat TI 623 tobaccolgbl621E tobacco 1970 33 87 98.2638 98.2578 blastp B426225_TI 889 397 624 tobaccolgbl621C tobacco 1971 33 95 99.3055 99.6515 blastp K720591_TI 556 679 625 tobaccolgbl621C tobacco 1972 33 96 73.6111 99.0654 blastp K720595_TI 111 206 626 tobaccolgbl621E tobacco 1973 33 88 98.2638 99.2982 blastp B427872_TI 889 456 627 tobaccolgbl621C tobacco 1974 33 87 97.9166 98.9473 blastp K720593_TI 667 684 628 tobaccolgbl621A tobacco 1975 33 95 99.3055 99.6515 blastp F024511_TI 556 679 629 tobaccolgbl621C tobacco 1976 33 96 98.9583 99.3031 blastp K720596_TI 333 359 630 tobaccolgbl621N tobacco 1977 33 96 98.9583 99.3031 blastp TU62280_TI 333 359 631 tomatolgbl641A tomato 1978 33 94 98.9583 99.3031 blastp W622243 TI 333 359 632 tomatolgbl641B tomato 1979 33 94 99.3055 100 blast G123213_TI 556 633 tomatolgbl641AI tomato 1980 33 87 98.2638 98.9473 blastp 637363_TI 889 684 634 tomatolgbl641B tomato 1981 33 85 98.2638 99.3006 blastp G123955_TI 889 993 635 tomatolgbl641BP tomato 1982 33 80 55.9027 86.8705 tblastn 876517_TI 778 036 636 triphysarialgbl6 triphysar 1983 33 86 73.6111 99.5305 blastp 41BM357654_TI ia 111 164 637 triphysarialgbl6 triphysar 1984 33 90 65.625 99.4736 blastp 41EY141207_TI ia 842 638 triphysarialgbl6 triphysar 1985 33 87 69.0972 100 blastp 41DR174621_TI ia 222 639 triphysarialgbl6 triphysar 1986 33 86 88.5416 99.6108 blast 41BM356761_TI ia 667 949 640 triphysarialgbl6 triphysar 1987 33 87 98.2638 99.6466 blastp 41DR169763_TI ia 889 431 641 triphysarialgbl6 triphysar 1988 33 86 96.5277 99.6428 blastp 41BM356902_TI ia 778 571 642 triphysarialgbl6 triphysar 1989 33 86 92.7083 97.4545 blastp 41DR174271_TI ia 333 455 643 triphysarialgbl6 triphysar 1990 33 88 100 99.6539 blastp 41DR171777 TI ia 792 62 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 644 wheatIgbI641BE wheat 1991 33 83 98.2638 98.9726 blastp 406715_TI 889 027 645 wheatlgbl641BQ wheat 1992 33 83 98.2638 98.9726 blastp 838456_TI 889 027 646 wheatIgbl641BE wheat 1993 33 82 98.2638 98.9726 blast 426386_TI 889 027 647 wheatIgbl641BE wheat 1994 33 88 51.0416 98.6577 blastp 403388_TI 667 181 648 wheatIgbl641BE wheat 1995 33 83 98.2638 98.9726 blastp 403307_TI 889 027 649 wheatIgbI641BE wheat 1996 33 81 60.4166 96.7213 blast 498268_TI 667 115 650 wheatIgbl641AL wheat 1997 33 84 84.7222 96.4705 blastp 828763_TI 222 882 651 wheatIgbI641AF wheat 1998 33 83 98.2638 98.9726 blastp 139816_TI 889 027 652 wheatIgbI641BE wheat 1999 33 86 98.2638 98.9583 blastp 216990_TI 889 333 653 wheatIgbI641BE wheat 2000 33 85 99.3055 99.6551 blast 403886_TI 556 724 654 wheatIgbI641BE wheat 2001 33 85 99.3055 99.6551 blastp 430165_TI 556 724 655 wheatIgbI641CA wheat 2002 33 87 56.9444 97.6190 blastp 484202_TI 444 476 656 wheatIgbI641BE wheat 2003 33 86 98.2638 98.9583 blast 404199_TI 889 333 657 wheatIgbI641BE wheat 2004 33 83 98.2638 98.9726 blastp 406086_TI 889 027 658 wheatIgbI641BF wheat 2005 33 83 98.2638 98.9655 blastp 293776_TI 889 172 659 wheatIgbI64ICK wheat 2006 33 81 97.2222 96.6216 blastp 193386_TI 222 216 660 castorbeanlgbI6 castorbea 2007 34 80 99.1902 98.7854 blastp 01AJ605572_TI n 834 251 661 citruslgb157.21C citrus 2008 34 80 99.1902 98.7854 blast K740163_TI 834 251 662 coffe4gbl5721D coffea 2009 34 80 100 100 blastp 663 lettucelgbl57.21 lettuce 2010 34 80 99.1902 99.5934 blast DW074942_TI 834 959 664 pepperlgbI5721 pepper 2011 34 95 76.1133 100 blastp BM063938_TI ppe 01 3 603 10 665 periwinklelgbI6 periwink 2012 34 81 99.1902 98.7903 blastp 41EG558295_TI le 834 226 666 potatolgbl57.21B potato 2013 34 98 94.7368 99.5744 blastp MI 12462_TI 421 681 667 tobaccolgbI621A tobacco 2014 34 95 100 100 blastp J237751_TI 668 tobaccolgbI621E tobacco 2015 34 93 100 100 blastp B425012_TI nicotiana bentha nicotiana 74.6177 97.5903 669 mianaIgbI62ICK _bentha 2016 35 86 37 614 blast 284579_TI miana 670 potatolgbl57.21B potato 2017 35 96 100 96.4497 blastp G594926_TI 041 63 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 671 tomatolgbl641D tomato 2018 35 90 76.1467 100 blastp B679435_TI 89 672 tobaccolgbl62|C tobacco 2019 36 81 53.5580 100 blastp 62 K720588_TI tbco 21 6 8 524 673 applelgb157.3|C apple 2020 37 84 85.7142 94.7368 blast N494715_TI 857 421 674 applegbI57.3|C apple 2021 37 81 94.2857 76.1538 blastp 0066689_TI 143 462 675 castorbeanlgb 6 castorbea 95.2380 36.9003 blast 65 OIMDL30026MO n 2022 37 90 952 69 bat 01488_TI 676 citruslgb157.21C citrus 2023 37 83 99.0476 72.7272 blast X300349_TI 19 727 677 coffealgbl57.21D coffee 2024 37 80 93.3333 34.5070 blastp V663640_TI 333 423 678 cowpealgbl66|F cowpea 2025 37 82 93.3333 95.1456 blastp F395821_TI 333 311 679 cowpealgbl661F cowpea 2026 37 82 94.2857 13.2450 tblastn F395821_T2 143 331 680 ipomoealgb157.2 ipomoea 2027 37 87 100 59.3220 blastp ICJ769054_TI 339 681 lettucelgbl57 .21 lettuce 2028 37 83 98.0952 36.5248 blastp CV699989_TI 381 227 medicago 1gb 157. mcdica 95.2380 37.1747 682 2IAW208262T cag 2029 37 80 952 212 blastp 1 683 melonIgbl651A melon 2030 37 82 97.1428 36.9565 blastp M727408_TI 571 217 684 poplarlgbl57.2|C poplar 2031 37 84 96.1904 36.5942 blastp N517706_TI 762 029 685 poplarlgb157.21B poplar 2032 37 84 99.0476 37.6811 blastp U813630_TI 19 594 686 potatolgbl57.21D potato 2033 37 90 96.1904 93.5185 blastp N587628_TI 762 185 687 ricelgb157.21B18 rice 2034 37 80 97.1428 35.9154 blastp 05522_TI 571 93 688 soybeanlgbI66|F soybean 2035 37 82 54.2857 98.2758 blastp K397604_TI 143 621 689 sunflowerlgbl621 sunflowe 2036 37 82 98.0952 39.0151 blast DY951259_TI r 381 515 690 sunflowerlgbI621 sunflowe 2037 37 83 98.0952 37.3188 blastp DY942645_TI r 381 406 691 triphysarialgbl6 triphysar 2038 37 85 99.0476 37.6811 blastp 41EY130232_TI ia 19 594 arabidopsislgb 16 arabido 767 602 692 51AT4G1380 T aidop 2039 38 80 97.6271 96.0526 blast 1 Ii 18 31 bat 693 artemisiagbI64 2040 38 87 55.5932 100 blastp 63 EY089420_TI atmsa 24 8 8 203 694 artemisiagbl641 artemisia 2041 38 89 51.8644 100 blastp 64 EY113317_TI atmsa 24 8 8 068 695 b-oleracealgbI6 bolerac 2042 38 81 93.8983 95.8620 blastp 1AM391026_Ti ea 051 69 696 b-rapalgbI62|C b rapa 2043 38 81 97.6271 96.0132 blastp V545128_Ti - 186 89 64 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 697 canolaIgbI6I1ES canola 2044 38 85 52.2033 100 blastp 903871_TI 898 698 cassavalgbI641C cassava 2045 38 89 93.8983 98.9247 blastp K641734_TI 051 312 699 castorbeanlgbI6 castorbea 2046 38 85 100 100 blast 01EG668085_TI n 700 castorbeanlgbI6 castorbea 2047 38 90 62.0338 100 blastp 01EG668085_T2 n 983 701 centaurealgbI611 centaure 2048 38 83 69.1525 98.0487 blastp EH739099_TI a 424 805 702 centaurealgbI611 centaure 2049 38 89 80.6779 95.9677 blastp EH710762_TI a 661 419 703 citruslgbl57.21C citrus 2050 38 98 62.0338 100 blast X299695_TI 983 704 citrusgb 1721C citrus 2051 38 80 100 100 blastp 705 citruslgb157.21C citrus 2052 38 81 78.3050 95.5465 blastp 0912981_T2 847 587 706 cottongbl641C0 cotton 2053 38 90 77.9661 95.4356 blast 071578_TI 017 846 707 cottonlgbl641BE cotton 2054 38 88 86.4406 100 blast 052767_TI 78 708 grapegb1 60ICB grape 2055 38 86 100 100 blast 350030_TI grp 205 386 10 10 bat 709 lettucelgb157.21 lettuce 2056 38 86 97.6271 96.6555 blast DW123895_TI 186 184 710 melonIgbI651A melon 2057 38 80 78.6440 92.8 blastp M726471_TI 678 nicotiana bentha nicotiana 711 mianaIgbI62ICK _bentha 2058 38 94 100 100 blastp 281387_TI miana 712 onionIgbI62|CF4 onion 2059 38 83 86.1016 98.0988 blast 36356_TI 949 593 713 poplarlgbl57 21B poplar 2060 38 84 97.6271 96.3333 blastp U895174_TI 186 333 714 26b5721B poplar 2061 38 85 100 100 blastp 715 radishgbI64|EX radish 2062 38 87 62.0338 100 blast 772276_TI 983 716 safflowergbI62 safflower 2063 38 87 55.5932 94.2528 blastp EL376221_TI 203 736 717 soybeanlgbI66|A soybean 2064 38 83 57.2881 100 blastp W351195_TI 356 718 spurgelgb16 D spurge 2065 38 81 66.1016 100 blastp 719 strawberrylgbI6 strawberry 2066 38 87 73.5593 100 blastp 41EX663538T1 y 22 720 sunflowergbl621 sunflowe 2067 38 83 69.8305 95.0636 tblastn BQ915292_T1 r 085 943 721 tobaccolgbI62|E tobacco 2068 38 94 100 100 blastp B426773_TI 1___1_ 722 triphysarialgbl6 triphysar 2069 38 90 67.7966 100 blastp 41EY008469_TI ia 102 723 Teirgb157.21 pepper 2070 39 91 60 100 blastp 65 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 724 tobaccolgbI62|E tobacco 2071 39 88 85.8333 100 blastp 74 B445778_TI toaco 201 39 8 725 pepperlgb15721 pepper 2072 40 82 64.4628 100 blastp CA5 15996_TI pepr 2740 8 099 10 726 potatogbl57.2 B potato 2073 40 92 100 100 blastp 727 potatolgbl57.21B potato 2074 41 100 79.4238 91.4691 blastp G887984_TI 683 943 728 applelgbI57.31C apple 2075 42 86 70.9677 98.0582 blastp N898142_TI 419 524 729 applelgbI57.31C apple 2076 42 82 99.6415 98.9399 blastp N492544_TI 771 293 730 applelgbI57.31C apple 2077 42 84 99.6415 98.9547 blastp N495819_TI 771 038 731 applelgbI57 31C apple 2078 42 86 100 100 blast N869175_TI apl 208 486 10 10 lat 732 applegbl57.3|C apple 2079 42 87 100 100 blastp 733 apricotlgbI57.21 apricot 2080 42 88 69.8924 98.4848 blastp CB820380_TI 731 485 734 aquilegiagb 2081 42 85 94.9820 100 blastp 31DR921860 TI aulga 28 2 8 789 10 arabidopsisgbl6 arabidop 99.2982 735 51AT2G37170_T sis 2082 42 80 100 456 blastp 1 arabidopsis|gb6 arabidop 95.6989 94.7552 736 51AT3G54820_T s 2083 42 81 247 448 blastp 1 arabidopsislgbl6 arabidop 99.3031 737 51AT3G53420_T sis 2084 42 81 100 359 blastp 1 arabidopsis|gb16 arabidop 99.2831 97.2508 738 51AT5G60660T a ois 2085 42 80 598 1 blast 740 artemisiagb1641 artemisia 208 42 8 79.5698 100 blastp EY056827Ti artemisia 2086 42 89 925 740 artemisialgbI64| artemisia 2087 42 83 100 100 blastp EY033689_TI 741 artemisialgbI64l reii 08 4 1 10 10 bat EY032199_TI aresa 208 481 10 00 bst 742 artemisialgbi64 artemisia 2089 42 88 100 100 blastp EY042731_TI 743 artemisiagbI64 artemisia 2090 42 82 99.6415 98.9473 blastp EX980079_TI 771 684 744 avocadolgbI641C avocado 2091 42 86 51.6129 97.2972 blast K754546_ a 032 973 b-juncealgbl641 99.2982 745 EVGN00454408 b-juncea 2092 42 81 100 456 blastp 761136_TI b-juncealgbl641 98.9247 97.9020 746 EVGN00454408 b-juncea 2093 42 81 312 979 blastp 761136_T2 b-juncealgbl641 88.7850 747 EVGN00748222 b-juncea 2094 42 80 100 467 blastp 952488_T2 66 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. bjuncealgb1641 82.0788 97.8991 748 EVGN00204411 b-juncea 2095 42 84 53 597 blastp 253360_TI bjuncealgbl641 99.2982 749 EVGN00054208 b-juncea 2096 42 80 100 456 blastp 600715_TI bjuncealgb1641 64.5161 96.2566 750 EVGN00049614 b-juncea 2097 42 83 29 845 blastp 332152_TI bjuncealgb1641 58.4229 751 EVGN01023711 b-juncea 2098 42 85 391 100 blastp 071914_TI bjuncealgbl641 99.3031 752 EVGN00247216 b-juncea 2099 42 81 100 359 blastp 171316_TI bjuncealgb1641 64.1577 753 EVGN00778009 b-juncea 2100 42 88 061 100 blastp 020884_TI bjuncealgb1641 53.4050 98.6754 754 EVGN02648808 b-juncea 2101 42 85 179 967 blastp 940517_TI bjuncealgbl641 82.7956 755 EVGN00316414 b-juncea 2102 42 82 989 100 blastp 413452_TI 756 b_7unceagb164 b-juncea 2103 42 81 100 99.3031 blastp bjuncealgb1641 99.3031 757 EVGN00748222 b-juncea 2104 42 81 100 359 blastp 952488_TI 758 b-oleracealgbI6 bolerac 2105 42 80 100 100 blastp 1|AM386520_TI ea 759 b-oleracealgbI6 bolerac 2106 42 83 57.3476 91.9540 blastp 1|AM058395_TI ea 703 23 760 b-oleracealgbI6 bolerac 2107 42 81 100 99.2982 blastp 1|AM385504_TI ea 456 761 bjrapagbI62|B b rapa 2108 42 81 100 100 blastp G544498_TI 762 b_a9 622 brapa 2109 42 81 100 99.2982 blastp 763 bjrapalgbI62|B b rapa 2110 42 81 100 99.2982 blastp Q791962 TI 456 764 bjrapalgb162|E b rapa 2111 42 81 92.1146 100 blastp X065729_TI - 953 765 bjrapalgbI62|C b rapa 2112 42 83 89.9641 98.8372 blastp A992278_TI - 577 093 766 bjrapagbI62|C b rapa 2113 42 81 100 99.2982 blastp 0749284_TI - 456 767 barle2gbl5731B barley 2114 42 81 100 100 blastp 768 beanIgbI64ICB5 bean 2115 42 80 100 100 blastp 42746_TI 769 beanIgbI64ICB2 bean 2116 42 86 99.6415 98.9473 blastp 80567_TI 771 684 770 beanlgbl64IBQ4 bean 2117 42 82 100 100 blastp 81649_TI ________________ ___________ 67 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 771 beanlgbl641CV5 bean 2118 42 86 99.6415 98.9547 blastp 32291_TI 771 038 brachypodiumg brachypo 94.9820 772 b161.xenolBE41 dium 2119 42 80 789 100 blastp 6137_TI brachypodiumg brachypo 773 b161.xenolAF13 dium 2120 42 80 100 100 blastp 9814_TI 774 canolalgbl6lCN canola 2121 42 81 100 99.3031 blastp 729066_TI 359 775 c 8olalgb161D canola 2122 42 81 100 99.2982 blastp 776 canolalgbl61IAF canola 2123 42 81 100 99.3031 blastp 118382_TI 359 777 canolalgbl6 IAF canola 2124 42 81 100 100 blastp 118383_TI1 778 canolalgbl6lCD canola 2125 42 80 100 99.2982 blastp 819509_TI 456 779 can4019 671_iEE canola 2126 42 81 100 100 blastp 780 canolalgbl61IEE canola 2127 42 81 100 99.2982 blastp 459735_TI 456 781 canolalgbl61|CX canola 2128 42 80 100 100 blastp 192356_TI 782 canolalgbl61|CN canola 2129 42 81 100 99.2982 blastp 827413_TI 456 783 canolalgbl61IEE canola 2130 42 81 100 99.2982 blastp 432011_TI 456 784 cassavalgbl64|D cassava 2131 42 81 93.9068 92.5266 blastp B922106_TI 1 904 785 caK64088_TllC cassava 2132 42 83 100 100 blastp 786 cassavalgbl641C cassava 2133 42 84 99.6415 98.9510 blastp K642866_TI 771 49 787 caK64251_TllC cassava 2134 42 86 100 99.3055 blast 788 castorbeanlgb16 castorbea 2135 42 86 100 99.3055 blastp 01AJ605565_TI n 556 789 castorbeanlgbl6 castorbea 2136 42 87 99.6415 98.9399 blast 01AJ605568_TI n 771 293 790 castorbeanlgbl6 castorbea 2137 42 85 100 100 blastp 01EE259660_TI n 791 centaurealgbl6l1 centaure 2138 42 84 87.0967 92.8571 blastp EL933765_TI a 742 429 792 centaurealgbl6l1 centaure 2139 42 82 75.9856 89.2561 blast EL931761_TI a 631 983 793 cichoriumlgbl61 cichoriu 2140 42 86 100 100 blastp IDT212328_TI m 794 cit Igb157.21B citrus 2141 42 83 100 100 blastp 795 citruslgb157.21C citrus 2142 42 86 100 99.3031 blast F509045_TI 2 42 359 796 citruslgbl57.21C citrus 2143 42 80 100 84.0707 blast B291797 TI 965 68 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 797 citruslgbl57.21B citrus 2144 42 86 100 99.3031 blastp Q623325_T1 359 798 citruslgbl57.21B citrus 2145 42 88 94.9820 99.2619 blastp Q623742_T1 789 926 799 citrus gbl57.21C citrus 2146 42 83 100 99.3031 blastp F417769_T1 359 800 citruslgb157.21B citrus 2147 42 88 68.4587 98.9637 blastp Q623128_T1 814 306 801 citruslgb157.21C citrus 2148 42 82 93.1899 98.8847 blastp B293000_TI 642 584 802 citruslgb157.21B citrus 2149 42 84 96.4157 98.5559 blast Q622991_T1 706 567 803 cit 13882_T1C citrus 2150 42 83 100 100 blastp 804 cottonlgbl641BE cotton 2151 42 84 99.2831 98.5815 blast 052942_TI 541 603 805 cottonlgbl641AF cotton 2152 42 80 100 100 blast 806 cottonlgbl641BG cotton 2153 42 85 100 99.2982 blastp 443494_TI 456 807 cottonlgbl641CO cotton 2154 42 88 70.2508 100 blast 086106_TI 961 808 cottonlgbl641BQ cotton 2155 42 82 100 99.2982 blastp 406033_TI 456 809 cottonlgb1641CO cotton 2156 42 82 100 100 blastp 810 cottonlgbl641DV cotton 2157 42 80 64.1577 95.3608 blast 437970_TI 061 247 811 cottonlgbl641AI cotton 2158 42 84 100 99.2982 blast 725803_TI 456 812 cottonlgbl641CD cotton 2159 42 81 98.9247 94.0199 blast 486305_TI 312 336 813 cowpealgb166|E cowpea 2160 42 85 86.3799 93.5606 blastp S884222_T2 283 061 814 cowpealgb166|E cowpea 2161 42 86 99.6415 98.9547 blastp S884222_TI 771 038 815 cowpealgbl66|F cowpea 2162 42 89 52.6881 98 blastp F538675_TI 72 816 C45811gb166|F cowpea 2163 42 80 100 100 blastp 817 cowpealgbl66|F cowpea 2164 42 85 99.6415 98.9473 blastp C458381_TI 771 684 cryptomerialgbl rpoe9.68 369 818 661AU036821T crypome 2165 42 83 95.6989 93.6395 blastp cryptomerialgbI rpoe5.06 803 819 661BW995927T crypome 2166 42 81 53.0465 98.0132 blastp 1 820 dandelionlgbl6l1 dandelio 2167 42 87 93.9068 88.9632 blastp DY827637_TI n 1 107 821 dandelionlgbl6l1 dandeio 2168 42 85 93.5483 93.2862 blastp DY814583_TI n 871 191 822 dandelionlgbl6l1 dandelio 2169 42 85 100 100 blastp DY828216 TI n 69 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 823 dandelionlgbl611 dandelio 2170 42 80 85.3046 98.7654 blastp DY818322_TI n 595 321 824 dandelionlgbI611 dandelio 2171 42 82 98.9247 98.2456 blastp DY805523_TI n 312 14 825 fescuelgbI61IDT fescue 2172 42 82 61.2903 93.9226 blast 675934_Ti 226 519 826 gingerlgbl641DY ginger 2173 42 81 100 100 blast 345807_TI gne 13 4 1 10 10 ba 827 gingerlgbI641DY ginger 2174 42 88 69.5340 100 blastp 373920_TI 502 828 grapeIgbI60IBQ grape 2175 42 81 100 99.3031 blastp 792080_TI 359 829 grapelgbI60ICB grape 2176 42 84 100 100 blast 973593_TI grp 216 484 10 10 bat 830 grapeIgbI60IBM grape 2177 42 84 100 99.2957 blastp 437196_TI 746 831 iceplantlgbI641C iceplant 2178 42 83 100 100 blast IPMIPCTi cpat 27 2 3 10 10 bat 832 iceplantlgbI641B iceplant 2179 42 82 99.6415 98.6254 blast E035661_TI 771 296 833 ipomoealgbI57.2 ipomoea 2180 42 80 99.6415 100 blastp IBM878800_TI iooa 28 2 8 771 10 834 ipomoealgbI57.2 ipomoea 2181 42 90 86.7383 93.2330 blastp IAU224434_T2 513 827 835 ipomoealgbI57.2 ipomoea 2182 42 82 100 99.2957 blastp IBJ553793_TI 746 836 ipomoealgbI57.2 ipomoea 2183 42 89 100 100 blast IAU224434_TI ipme 213 489 10 00 bat 837 lettucelgb 157.21 lettuce 2184 42 85 99.2831 98.5964 blastp DW115660_TI 541 912 838 lettucelgbl57.21 lettuce 2185 42 80 97.8494 95.7894 blastp DW113963_TI 624 737 839 lettucelgbl57.21 lettuce 2186 42 84 99.6415 98.9399 blastp DW110249_TI 771 293 840 DW051453_T1 lettuce 2187 42 86 100 100 blastp 841 Dt 7 507_T1 lettuce 2188 42 84 100 100 blastp 84 Wltt7eg657 TI21 842 DW12617_T1 lettuce 2189 42 84 100 100 blastp 843 lettucelgbl57.21 lettuce 2190 42 80 100 100 blastp 844 lettucelgbl57.21 lettuce 2191 42 83 92.8315 98.5018 blastp DW049421_TI 412 727 845 lettucelgb157.21 lettuce 2192 42 87 99.2831 98.9473 blast DW114305_TI 541 684 846 D c 5722_T1 lettuce 2193 42 80 100 100 blastp 847 D I 1578_T1 lettuce 2194 42 81 100 100 blastp 848 lettucelgbl57.21 lettuce 2195 42 85 99.2831 98.5964 blastp DW077710_TI 541 912 849 DW070566_T1 lettuce 2196 42 84 100 100 blastp 70 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 850 lettucelgbI57 .21 lettuce 2197 42 85 94.9820 100 blast DW093078_TI 789 851 lettucelgb157.21 lettuce 2198 42 84 97.4910 96.1672 blastp DW074446_TI 394 474 852 lettucelgb157.21 lettuce 2199 42 83 99.6415 98.9399 blast DW153482_TI 771 293 853 Dc 5306_T1 lettuce 2200 42 86 100 100 blastp 854 lettucelgbl57.21 lettuce 2201 42 84 99.6415 98.9399 blastp DW043674_TI 771 293 855 lettucelgb157.21 lettuce 2202 42 83 97.1326 98.5559 blast DW095979_TI 165 567 856 Du 17206_T1 lettuce 2203 42 84 100 100 blastp 857 lettucelgb157.21 lettuce 2204 42 86 98.9247 99.6453 blastp DW047573_TI 312 901 858 Dt 9 304_T1 lettuce 2205 42 87 100 100 blastp 859 D 7 191_Ti lettuce 2206 42 81 100 100 blastp 860 lotuslgbl57.21AI lotus 2207 42 85 99.6415 98.9547 blastp 967757_Ti 771 038 861 lotuslgbl57.21AI lotus 2208 42 80 99.6415 99.6515 blastp 967387_T2 771 679 862 lotuslgbI57.21B lotus 2209 42 82 99.6415 98.9619 blastp G662315_TI 771 377 863 maizeIgbI641BE maize 2210 42 80 97.4910 95.8904 blastp 552783_TI 394 11 864 maizelgbl641AI6 maize 2211 42 83 97.4910 95.8620 blast 22334_TI 394 69 865 maizelgb1641AI8 maize 2212 42 81 96.7741 95.1557 blastp 55280_TI 935 093 medicagolgbl57. medicag 99.3031 866 21AW981259_T 0 2213 42 80 100 359 blastp 1 867 medicagolgbI57. medicag 2214 42 83 99.6415 98.9547 blastp 21AA660788_TI 0 771 038 868 melonIgbI651DV melon 2215 42 84 100 100 blastp 631824_TI 869 melonIgbI651DV melon 2216 42 83 64.5161 100 blastp 633977_Ti 29 870 melonIgbI651A melon 2217 42 81 86.0215 100 blastp M720039_TI 054 nicotiana bentha nicotiana 871 mianaIgbI62ICN _bentha 2218 42 80 100 100 blastp 743200_TI miana nicotiana bentha nicotiana 98.2078 96.8641 872 mianaIgbI62ICK _bentha 2219 42 80 853 115 blast 294539_TI miana 873 onionIgbI621AF onion 2220 42 82 97.1326 95.5326 blastp 255795_TI 165 46 874 onionIgbI62|CF4 onion 2221 42 80 99.2831 99.2957 blastp 34704_TI 541 746 875 papayalgbI65|E papaya 2222 42 82 82.0788 97.5206 blastp L784273 TI 53 612 71 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 876 papayalgbI651A papaya 2223 42 84 100 99.2882 blastp M904340_TI 562 877 peachlgb157.21A peach 2224 42 84 87.0967 100 blastp F367458_TI 742 878 peachlgb157.21B peach 2225 42 83 99.6415 98.9547 blast U040116_TI 771 038 879 peachlgbI57.21A peach 2226 42 86 87.0967 100 blastp F367460_TI 742 880 peanutlgbl61 IC peanut 2227 42 86 99.6415 98.9547 blastp D037924_TI 771 038 881 peanutlgbl61IC peanut 2228 42 85 99.6415 98.9547 blastp D038296_TI 771 038 882 peanutlgbl61 IC peanut 2229 42 86 99.6415 98.9547 blastp D037924_T2 771 038 883 peanutlgbl61IC peanut 2230 42 88 51.2544 97.9452 blastp D037884_TI 803 055 884 BMe66rgb15721 pepper 2231 42 96 100 100 blastp 885 BMe66rgb15721 pepper 2232 42 97 100 100 blastp 886 pepperlgbI57.21 pepper 2233 42 81 92.4731 97.3977 blastp BM061005_TI 183 695 887 petunialgb157.21 petunia 2234 42 83 97.4910 98.2332 blastp AF452014_TI 394 155 888 petunialgb157.21 petunia 2235 42 95 53.0465 93.6708 blastp CV295523_TI 95 861 889 peunialgb57.21 petunia 2236 42 83 55.5555 100 blastp CV293001_TI ptna 23 2 8 556 10 890 pinelgbI57.21AI8 pine 2237 42 81 96.0573 94.3262 blastp 13221_TI 477 411 891 pinelgbI57.2|CA pine 2238 42 81 56.6308 98.75 blast 844411_TI 244 892 poplarlgbI57.21B poplar 2239 42 85 100 99.2982 blastp 1130501_T3 456 893 poplarlgbI57.21A poplar 2240 42 86 99.6415 98.9473 blastp 1165755_Ti 771 684 894 poplarlgb157.21B poplar 2241 42 85 99.6415 98.9473 blastp U835712_TI 771 684 895 poplarlgbI57.21B poplar 2242 42 85 100 99.2982 blastp 1130501_TI 456 896 poplarlgbI57.21B poplar 2243 42 85 100 99.2982 blastp 1130501_T4 456 897 poplarlgb157.21A poplar 2244 42 86 99.2831 98.5964 blastp 1162288_TI 541 912 898 5b572IA poplar 2245 42 84 100 100 blastp 899 potato gb157.21 potato 2246 42 96 100 100 blastp 900 potatolgb157.21B potato 2247 42 80 98.2078 96.8641 blastp M109370_TI 853 115 901 potatolgb157.21B potato 2248 42 80 98.2078 96.8641 blastp G589618_TI 853 115 902 potatolgb157.21C potato 2249 42 83 70.2508 100 blastp K261080 TI 961 72 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 903 potato lgb57.21B potato 2250 42 97 99.2831 100 blastp G098124_TI ptt 20 4 541 10 904 potatolgbl57.21B potato 2251 42 80 98.2078 96.8641 blastp 1406400_TI 853 115 905 t2 lbl5721B potato 2252 42 90 100 100 blastp 906 potatolgb157.21B potato 2253 42 80 98.2078 96.8641 blastp M112017_T1 853 115 907 t2 67921B potato 2254 42 96 100 100 blastp 908 potatolgb157.21B potato 2255 42 80 98.2078 96.8641 blast G600158_TI 853 115 909 40tatogb157.21B potato 2256 42 80 100 100 blastp 910 potatolgb157.21C potato 2257 42 80 98.2078 96.8641 blastp K719282_TI 853 115 911 radishlgbl64|EV radish 2258 42 81 100 99.2982 blastp 545956_TI 456 912 radishlgbl64|EX radish 2259 42 80 100 100 blast 904869_TI radish 2259 42 80 100 100 blast 913 radishlgbl64|EV radish 2260 42 80 100 100 blastp 545247_TI 914 radishlgbi64lEX radish 2261 42 80 100 100 blastp 756889_TI 915 radishlgbl64|EV radish 2262 42 81 100 99.3031 blast 539533_TI 359 916 radishlgbl64|EV radish 2263 42 82 55.5555 95.6790 blastp 546186_TI 556 123 917 radishlgbl641AB radish 2264 42 81 100 99.3031 blastp 012045_TI 359 918 radishlgbl64|EV radish 2265 42 83 62.0071 98.8571 blastp 573001_TI 685 429 919 radishlgbl641AB radish 2266 42 81 100 100 blastp 030698_TI 920 radishlgbl64|EX radish 2267 42 83 78.4946 100 blastp 749049_TI 237 921 radishlgbi641AB radish 2268 42 80 100 100 blastp 030697_TI 922 radishgbl64|EW radish 2269 42 80 96.4157 95.4545 blast 735060_TI 706 455 923 radishlgbl64|FD radish 2270 42 83 50.5376 100 blastp 936119_TI 344 924 radishlgbl64|EV radish 2271 42 81 100 99.3031 blastp 543747_TI 359 925 ricelgb157.21BE0 rice 2272 42 80 86.7383 94.3820 blast 40651_T2 513 225 926 rice406517.21BE rice 2273 42 80 100 100 blastp 927 ricelgb157.21AA rice 2274 42 80 85.6630 87.7192 blast 754435_T5 824 982 928 riceb157.24 A rice 2275 42 81 100 100 blastp 929 roselgb157.21BI9 rose 2276 42 84 69.8924 91.6279 blastp 77420 TI 731 07 73 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 930 roselgbl57.21BI9 rose 2277 42 84 77.7777 100 blastp 77750_TI 778 931 roselgb157.21BI9 rose 2278 42 83 64.5161 98.3783 blastp 78110_TI 29 784 932 safflowerlgb1621 safflower 2279 42 81 98.5663 100 blastp 92 EL406648_TI sflwr 27 2 8 082 933 safflowerlgbl621 safflower 2280 42 86 92.4731 97.7611 blastp EL411110_TI 183 94 934 safflowerlgbl621 safflower 2281 42 85 100 100 blastp EL401452_TI 935 saf werlgb1621 safflower 2282 42 83 9716326 100 blastp 936 safflowerlgbl621 safflower 2283 42 85 86.7383 67.9193 tblastn EL400227_TI 513 401 sorghumlgb161. 97.4910 95.8620 937 xenoIAI622334_ sorghum 2284 42 83 394 69 blastp TI 938 soybeanlgbl661C soybean 2285 42 80 99.6415 99.6515 blastp D393286_TI 771 679 939 soybeanlgbl661G soybean 2286 42 86 99.6415 98.9473 blastp MU27347_TI 771 684 940 soybeanlgbl661A soybean 2287 42 85 99.6415 98.9473 blastp W349289_TI 771 684 941 soybeanlgbl66|B soybean 2288 42 87 99.6415 98.9436 blast E823946_TI 771 62 942 soybeanlgbl66|B soybean 2289 42 80 56.2724 100 blastp E352729_T2 soba 29 4 0 014 10 943 soybeanlgbl661A soybean 2290 42 85 99.6415 98.9547 blastp W350475_TI 771 038 944 so1 b66|B soybean 2291 42 80 100 100 blastp 945 soWbeanlgb661A soybean 2292 42 80 100 100 blastp 946 sprucelgbl621AF spruce 2293 42 81 96.0573 94.3262 blastp 051202_TI 477 411 947 sprucelgb1621CO spruce 2294 42 80 96.0573 93.6619 blast 227554_TI 477 718 948 sprucelgb1621CO spruce 2295 42 80 96.4157 94.6808 blastp 220480_TI 706 511 949 sprucelgb1621CO spruce 2296 42 80 96.0573 94.3262 blastp 217151_TI 477 411 950 spurgelgbl 61 A spurge 2297 42 80 59.8566 91.5343 blastp W990929_TI 308 915 951 spurgelgb16 B spurge 2298 42 83 100 100 blastp 952 spurgelgbl61 ID spurge 2299 42 87 83.5125 99.5780 blastp V125170_TI 448 591 953 strawberrylgbl6 strawberry 2300 42 85 99.6415 98.9473 blastp 41DV438166T1 y 771 684 954 strawberrylgbl6 strawberry 2301 42 86 100 99.2957 blastp 41EX665494_T1 y 746 955 strawberrylgbl6 strawberry 2302 42 85 100 100 blastp 41CO817390_T1 y 74 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. sugarcanelgbl57 sugarcan 70.2508 956 .21BQ536871_T s 2303 42 85 961 100 blast 2 sugarcanelgb15 7 ca 957 nU g sugarcan 2304 42 83 97.4910 92.6666 97 . 1BU058 e 234 4 3 394 667 blastp 1 958 sugarcanelgbl57 can 97.4910 95.8620 blast 98 .21BQ536871_T sua 2305 42 81 394 69 bat 1 sugarcanelgb15 7 suacan 97.4910 95.8620 959 .21BQ535332T sugar 2306 42 84 394 69 blastp 1 960 sunflowerlgbl621 sunflowe 2307 42 83 100 100 blastp CD849689_TI r 961 sunflowerlgbl621 sunflowe 2308 42 83 94.9820 95.0704 blastp CD849494_TI r 789 225 962 sunflowerlgbl621 sunflowe 2309 42 81 83.5125 98.75 blastp CF091932_TI r 448 963 sunflowerlgbl621 sunflowe 2310 42 81 100 100 blastp DY915644_TI r 964 sunflowerlgbl621 sunflowe 2311 42 81 78.4946 88.8446 blastp EL462160_TI r 237 215 965 sunflowerlgbl621 sunflowe 2312 42 87 95.6989 100 blastp DY918039 TI r 247 966 sunflowerlgbl621 sunflowe 2313 42 87 89.9641 95.5223 blastp DY951506_TI r 577 881 967 sunflowerlgbl621 sunflowe 2314 42 84 99.6415 98.9473 blastp DY933519_TI r 771 684 968 sunflowerlgbl621 sunflowe 2315 42 82 100 100 blastp DY917102_TI r 969 sunflowerlgbl621 sunflowe 2316 42 86 58.0645 100 blast DY932916_TI r 161 970 sunflowerlgbl621 sunflowe 2317 42 84 100 100 blastp CD857425_TI r 971 sunflowerlgbl621 sunflowe 2318 42 85 53.4050 97.4683 blastp CD846314_TI r 179 544 972 sunflowerlgbl621 sunflowe 2319 42 88 68.1003 89.6226 blastp CX944368_TI r 584 415 973 sunflowerlgbl621 sunflowe 2320 42 80 100 100 blastp DY908592_TI r 974 switchgrasslgbl6 switchgr 2321 42 81 96.7741 98.9208 blastp 51DN141399_TI ass 935 633 975 switchgrasslgbl6 switchgr 2322 42 86 51.9713 99.3150 blastp 51FE621421_TI ass 262 685 976 switchgrasslgbl6 switchgr 2323 42 83 97.4910 95.8620 blastp 51DN145656_TI ass 394 69 977 switchgrasslgbl6 switchgr 2324 42 81 82.4372 100 blastp 51FE637674_TI ass 76 978 switchgrasslgbl6 switchgr 2325 42 83 77.0609 94.8497 blastp 51DN141334_TI ass 319 854 979 switchgrasslgbl6 switchgr 2326 42 83 85.6630 100 blastp 51FE621578_TI ass 824 thellungiellalgbl thellungi 72.4014 980 57.21DN775526 ella 2327 42 81 337 100 blast TI IIIII 75 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 981 tobaccolgbI62|C tobacco 2328 42 95 100 100 blastp K720599 TI 982 tobaccolgbI621C tobacco 2329 42 94 100 100 blastp K720599_T2 984 tobcc1|gb162|E tobacco 2330 42 81 100 100 blastp 984 tobaccolgbI621E tobacco 2331 42 89 100 100 blastp B443112_TI 985 tobaccolgbI621A tobacco 2332 42 80 100 100 blastp F154641_TI 96 tobaccolgbl62lE toac 33 4 4 98.2078 100 blastp 96 B425288_TI toac 233 4 853 987 tomatolgbl641B tomato 2334 42 81 92.8315 97.4074 blastp G123951_T2 412 074 988 tomatolgbl641B tomato 2335 42 80 98.2078 96.8641 blastp G123951_TI 853 115 989 tomatolgbl641B tomato 2336 42 92 53.4050 98.0519 blast G713781_TI 179 481 990 tomatolgbl641A tomato 2337 42 81 97.1326 98.2142 blastp W219533_TI 165 857 991 triphysarialgbl6 triphysar 2338 42 88 99.2831 99.2857 blastp 41DR170852_TI ia 541 143 992 triphysarialgbl6 triphysar 2339 42 88 64.8745 100 blastp 41BM356582_TI ia 52 993 triphysarialgbl6 triphysar 2340 42 80 100 100 blast 41BE574767_TI ia 994 wheatIgbI641BE wheat 2341 42 82 55.5555 100 blastp 444481_TI 556 995 wheatIgbI641BE wheat 2342 42 81 100 100 blastp 398316_TIII 996 wheatIgbI641BE wheat 2343 42 82 51.6129 99.3055 blastp 404002_TI 032 556 997 applegbl573|C apple 2344 43 84 100 100 blastp 998 applel531A apple 2345 43 85 100 100 blastp 999 Taiuegialgb157. aquilegia 2346 43 84 100 100 blastp arabidopsisgbl6 arabo 1000 51AT2G16850_T idop 2347 43 84 100 100 blastp 1 arabidopsisgbl6 arabi 1001 51AT4G35100_T idop 2348 43 86 100 100 blastp 1 1002 avocadolgbI641C avocado 2349 43 85 66.4310 92.6108 blastp K753629_TI 954 374 1003 avocadolgbI641C avocado 2350 43 84 62.1908 100 blastp 103 K752541_TI aoao 253 8 127 b-juncealgbl641 67.1378 1004 EVGN01060535 b-juncea 2351 43 86 092 100 blastp 361904_TI b-juncealgbl641 85.1590 1005 EVGN00138211 b-juncea 2352 43 86 106 100 blastp 060167 TI 76 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. b-juncealgbl641 66.0777 1006 EVGN01177009 b-juncea 2353 43 86 385 100 blastp 332048_TI b-juncealgbl641 90.4593 1007 EVGN00071418 b-juncea 2354 43 86 64 100 blastp 640425_TI b-juncealgbl641 53.0035 1008 EVGN01391814 b-juncea 2355 43 92 336 100 blastp 101746_TI b-juncealgbl641 1009 EVGN00206114 b-juncea 2356 43 85 100 100 blastp 600060_TI 1010 boleracealgbl6 bolerac 2357 43 85 100 100 blastp IIAF314656_TI ea 1011 b-oleracealgbl6 bolerac 2358 43 86 100 100 blastp 1|DY029187_TI ea 1012 b-oleracealgbl6 bolerac 2359 43 87 54.7703 100 blastp 1|DY014978_TI ea 18 1013 bjrapalgbl621B b rapa 2360 43 86 77.0318 97.7375 blastp Q791230T1 - 021 566 1014 bjrapalgbl62|E b rapa 2361 43 87 100 100 blastp X033370T6 b 1015 b-rapalgb1621C brapa 2362 43 86 100 100 blastp 1016 beapalgbl62 B brapa 2363 43 85 100 100 blastp G5439061TD c 1017 beanlgbl641CBD bean 2364 43 83 100 100 blastp 39790_TI 1018 beetlgbl621BVU beet 2365 43 80 100 100 blastp 60148_TI 1019 canolalgbl61CD canola 2366 43 86 100 100 blastp 1020 canolalgbl61|CD canola 2367 43 85 100 100 blastp 815284_TI 1021 canolalgbl64CD canola 2368 43 85 100 100 blastp 817684_TI 1022 canolagbl6ICD canola 2369 43 86 100 100 blastp 821191_TI 1026 casoralgbl6837_ canola 2370 43 87 100 100 blastp 1024 cassavalgb641B cassava 2371 43 84 100 100 blastp M259748_TI 1025 castorbeanlgb 6 castorbea 2372 43 84 100 100 blastp OIAJ605569_TI n 1030 castorbeanlgbl6 castorbea 237 43 8 69.9646 95.1219 blastp 1AJ605569_T2 n4 107 cichoriumlgbi6i cichoriu 234 4 9 67.1378 100 blastp 107 IEH704748_TI m 34 3 8 092 1028 citus2b15271B citrus 2375 43 85 100 100 blastp 1029 citrs496415.Ti citrus 2376 43 84 100 100 blastp 100 cloverlgbi62IBB coe 237 482 54.0636 100 blastp 100 911260 TI clvr 2743 8 042 77 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 1031 coffealgbl57 21B coffea 2378 43 86 100 100 blastp 1032 cottonlgbl641AI cotton 2379 43 84 97.8798 88.0258 blast 726086_TI 587 9 1033 cottonlgbl641C0 cotton 2380 43 82 92.2261 95.5719 blast 098535_TI 484 557 1034 cottongbl641BE cotton 2381 43 83 100 100 blastp 1035 cottonlgbl641BQ cotton 2382 43 85 59.0106 100 blastp 414250_TI 007 1036 cottonlgbl641BF cotton 2383 43 87 90.1060 100 blast 268907_TI 071 1037 cottonlgbl641C0 cotton 2384 43 84 62.5441 100 blast 075847_TI 696 1038 cottonlgbl641BQ cotton 2385 43 82 95.4063 95.7142 blastp 405584_TI 604 857 1039 cottongbl641AI cotton 2386 43 85 100 100 blastp 1040 cowpealgbl66|F cowpea 2387 43 84 100 100 blastp 1041 dandelionlgbI611 dandelio 2388 43 85 100 100 blastp DY803814_TI n 1042 gingergb64DY ginger 2389 43 80 100 100 blastp 1043 gingerlgbI64|DY ginger 2390 43 84 94.6996 92.7335 blastp 347296_TI 466 64 1044 gingergbl64DY ginger 2391 43 81 100 100 blastp 1045 gingergbl641DY ginger 2392 43 81 100 100 blastp 1046 gingerlgbI64|DY ginger 2393 43 81 81.9787 93.9024 blastp 360032_TI 986 39 1047 grapelgbl60 M grape 2394 43 84 100 100 blastp 108 3ce79tl1641 1048 n g iceplant 2395 43 82 100 100 blastp 1049 ipomoealgbI57.2 ipomoea 2396 43 83 100 100 blast 1050 D c 59_1 lettuce 2397 43 84 100 100 blastp 1051 lettuce 2398 43 84 97.8798 100 blast 1052 D 059_Ti1 lettuce 2399 43 84 100 100 blastp 1053 lettucegbI57 21 lettuce 2400 43 83 97.1731 100 blast 101 DW1102253TI le4c42993 4 57 0 1054 7 21 lettuce 2401 43 84 100 100 blastp DW49482_TI 1055 lettucelgbI57.21 lettuce 2402 43 83 97.5265 92.8327 blast DW094572_T1 018 645 1056 lotusgb157.21A lotus 2403 43 85 54.4169 93.9024 blastp W163949_TI 611 39 1057 lotusgb157.21AI lotus 2404 43 82 98.5865 97.5352 blast 96757_T 724 113 78 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 1058 melonIgb 65IDV melon 2405 43 83 100 100 blastp 632217_-TI 1059 o a g oil-palm 2406 43 80 100 100 blastp 106007 Taaag I6l 1060 pXayalgb165|E papaya 2407 43 85 100 100 blastp 1061 peachlgbI57.21A peach 2408 43 85 85.1590 99.5833 blastp F367457_TI 106 333 1062 pepperlgbI57.21 pepper 2409 43 96 67.4911 98.9637 blastp BM066074_TI 661 306 1063 pepperlgbI5721 pepper 2410 43 88 53.7102 100 blastp CA518686_TI pepr 240 438 0 1064 periwinklelgbI6 periwink 2411 43 90 100 100 blastp 41AM232518_TI le 1065 p n petunia 2412 43 89 100 100 blastp 1066 b15721A poplar 2413 43 84 100 100 blastp 107 1opar357TIA 1067 15721A poplar 2414 43 83 100 100 blastp 1068 potatolgb157.21B potato 2415 43 89 83.7455 100 blastp F053675_TI 83 1069 potatolgbl57.21B potato 2416 43 97 100 100 blast F459952_TI ptt 46 4 7 10 10 ba 1070 radishgbl645EV radish 2417 43 86 100 100 blastp 1071 radishlgbI64|EV radish 2418 43 85 100 100 blastp 539317_TI 1072 radishlgbI64|EV radish 2419 43 85 100 100 blastp 525026_TI 1073 roselgbI57.21BQ rose 2420 43 82 100 100 blastp 103996_TI 1074 safflowerlgbI621 safflower 2421 43 83 100 100 blastp EL372747_TI 1075 sesamelgbI5721 sesame 2422 43 88 50.8833 100 blastp 105 BU669158_TI seae 22 43 8 922 1076 sesamelgbI5721 sesame 2423 43 87 51.9434 100 blastp 106 BU668646_TI seae 23 43 8 629 1077 so1beangbi661B soybean 2424 43 82 100 100 blastp 108 syEan3128 16Il 1078 soybean 1661B soybean 2425 43 82 100 100 blastp 1079 sprucelgbI62|CO spruce 2426 43 80 93.2862 93.5714 blastp 217407_TI 191 286 1080 spurgelgbl 6lA spurge 2427 43 84 100 97.2125 blastp W821924_TI 436 1081 strawberrylgbI6 strawberry 2428 43 82 100 100 blastp 41CX661107T1 y 1082 sunflowerlgbI621 sunflowe 2429 43 85 100 100 blastp CD853582_TI r 1083 sunflowerlgbI621 sunflowe 2430 43 80 97.1731 97.8339 blastp DY939653_TI r 44935 1084 sunflowerlgbI621 sunflowe 2431 43 81 100 100 blast CD849663 TI r 79 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. thellungiellalgbI thellungi 2432 43 86 72.0848 90.5829 blast 1085 57.21DN777165 ella 242 4 6 057 596 bat TI 1086 tobaccolgbI62|C tobacco 2433 43 90 99.6466 100 blast 106 K720587_TI tbco 23 3 9 431 10 bat 1087 tobaccolgbI62|C tobacco 2434 43 90 100 100 blastp K720585_TI 1088 tobaccogbI62C 2435 43 96 59.7173 100 blastp 108 V016422_TI tbco 23 3 9 145 1089 tobaccolgbI62|C tobacco 2436 43 94 100 100 blastp K720589_TI 1090 toGatolgb1641B tomato 2437 43 88 100 100 blastp 1091 triphysarialgbl6 triphysar 2438 43 83 100 100 blastp 41EY018490_TI ia 1092 triphysarialgbl6 triphysar 2439 43 84 100 100 blastp 41EY007858_TI ia 1093 applelgbI57.31C apple 2440 44 80 79.1390 93.0232 blastp N494428_TI 728 558 1094 castorbeanlgbI6 castorbea 2441 44 82 99.0066 97.7272 blastp 01EG696741_TI n 225 727 1095 citruslgb157.21C citrus 2442 44 82 99.0066 97.6973 blastp X074332_TI 225 684 1096 citruslgb157.21C citrus 2443 44 80 86.7549 85.8085 blast X674035_TI 669 809 1097 cottonlgbl641D cotton 2444 44 80 99.6688 98.3333 blast W234737_TI 742 333 1098 D 6 21 lettuce 2445 44 80 95.0331 100 blastp DW066284_TI12 1099 petunialgb57.21 petunia 2446 44 84 65.8940 100 blastp CV298254_TI pe3ia 246 478 0 1100 potatolgb157.21C potato 2447 44 94 72.1854 99.5433 blast V500020_TI 305 79 1101 tobaccolgbI621E tobacco 2448 44 89 99.0066 97.6897 blastp B426672_TI 225 69 brachypodiumg brachypo 1102 b161.xenoIBE41 dium 2449 46 92 100 100 blastp 5047_TI 1103 maizeIgbI641CF maize 2450 46 90 90.7630 100 blast 244342_TI 522 1104 maize1641AF maize 2451 46 89 100 100 blastp sorghumgbl61. 1105 xenolAF057183_ sorghum 2452 46 88 100 100 blastp TI sugarcanelgbI57 can 1106 .2CA132045_T sugar 2453 46 89 100 100 blastp 1 1107 switchgrasslgbI6 switchgr 2454 46 90 100 100 blastp 51FE617713_TI ass 1108 wheatIgbI641BE wheat 2455 46 95 100 100 blastp 430088_TI a e68 s 1109 applelgbI57.31D apl 46 4 1 87.9518 10 blastp T001281 TI ape 25 7 8 072 10 80 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. brachypodiumig brachypo 1110 b161.xenolBE40 dium 2457 47 95 100 100 blastp 2447_TI 1111 gingerlgbl64|DY ginger 2458 47 88 64.2570 98.7654 blastp 364894_TI 281 321 1112 maizeb1641AI8 maize 2459 47 89 100 100 blastp 1113 W017703_Ti maize 2460 47 85 100 100 blastp 1114 onionlgbl62|AC onion 2461 47 83 99.5983 99.5967 blastp U58207_TI 936 742 1115 onionlgbl62|CF4 onion 2462 47 85 95.9839 98.75 blast 37464_TI 357 1116 oniongbl621CF4 onion 2463 47 84 100 100 blastp 1117 ric03157.2 U rice 2464 47 91 100 100 blastp 1118 ricelgb157.2|CA rice 2465 47 87 97.5903 31.6406 blastp 756239_TI 614 25 sorghumgbl61. 1119 xenoIAI855402_ sorghum 2466 47 89 100 100 blastp TI sugarcanelgb57 2can 99.5983 99.5967 blast 1120 .21CA132045_T sua 2467 47 90 936 742 bat 2e 1121 switchgrasslgbl6 switchgr 2468 47 90 100 100 blastp 51FE624217_TI ass 1122 wheat|gbi641BE wheat 2469 47 97 100 100 blastp 404100_TI 1123 fescuelgbl6llDT fescue 2470 48 94 100 100 blastp 700572 TI 1124 gingerlgbl64|DY ginger 2471 48 80 95.1612 96.7346 blast 361836_TI 903 939 1125 ricelgbl572iU37 rice 2472 48 90 100 100 blastp 1126 ricelgb157.2|U37 rice 2473 48 89 51.6129 89.5104 blastp 952_T3 032 895 1127 ryelgb1641BE49 rye 2474 48 98 80.6451 100 blast 5605_TI 613 sorghumgbl61. 1128 xenoIAI724211 sorghum 2475 48 90 100 100 blastp TI sugarcanegb57 sugarcan 92.3387 1129 .2|CAI 10414_T s 2476 48 82 097 100 blast 1e sugarcanelgbl57 suacan9032 9651 1130 .21CA065356 T sugar 2477 48 83 03225 96.5517 blast 1 sugarcaneb57 sugarcan 71.3709 95.6756 blast 1131 .21CA143208 T sua 2478 48 83 677 757 bat 1 sugarcanelgbl57 can 1132 .21CA101765_T sugar 2479 48 91 100 100 blastp 1 81 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. sugarcanelgbl57 sugarcan 83.4677 95.8333 blast 1133 .21CA133231_T sua 2480 48 90 419 333 bat 1 1134 switchgrasslgbI6 switchgr 2481 48 91 100 100 blastp 51FE605472_TI ass 1135 wheatIgbI641BE wheat 2482 48 95 100 100 blastp 489764_TI 1136 wheatIgbl641TA wheat 2483 48 95 100 100 blastp U86763_TI 1137 wheatIgbl64IBE wheat 2484 48 95 100 100 blastp 415001_-TI b-juncealgbl641 85.0931 1138 EVGN00504508 b-juncea 2485 50 80 677 100 blastp 791211_TI b-juncealgbl641 50.9316 91.9540 1139 EVGN01684214 b-juncea 2486 50 84 77 23 blastp 261870_TI 1140 bananalgbl601D banana 2487 50 84 80.1242 91.9708 blastp N239388_TI 236 029 1141 barleylgbl57.31B barley 2488 50 91 58.3850 98.9690 blastp F253694_Ti 932 722 1142 barleylgbl57.31B barley 2489 50 95 100 62.6923 blastp E412959_T3 077 1143 beanIgb164|FD7 bean 2490 50 82 100 91.9075 blastp 93482_TI 145 1144 canolaIgbI6I|CX canola 2491 50 83 99.3788 66.9527 blastp 193398_T3 82 897 1145 canolaIgbl61IEV canola 2492 50 81 52.7950 91.2087 blastp 123336_TI 311 912 1146 centaureagbI611 centaure 2493 50 81 98.1366 62.2489 blastp EL931277 TI a 46 96 1147 cichoriumlgbI61 cichoriu 2494 50 83 64.5962 95.3271 blast IDT213939_TI m 733 028 1148 fesc3gbl6 DT fescue 2495 50 99 100 946520 blast 1149 lotusgbl57.21B1 lotus 2496 50 86 98.1366 88.7005 blastp 418499_Ti 46 65 1150 onionIgbI62IBQ onion 2497 50 86 100 57.1942 blast 579939_TI 446 1151 peachlgbI57.21B peach 2498 50 82 98.1366 56.2043 blastp U040795_T1 46 796 1152 peachlgbI57.21D peach 2499 50 83 98.1366 91.8128 blastp W351857_TI 46 655 1153 ryelgb1641BF429 rye 2500 50 88 93.1677 100 blast 463_TI 019 1154 soybeanlgbI66|B soybean 2501 50 81 98.1366 70.7207 blast E352747_T4 46 207 sugarcanelgbI57 sugarcan 98.1366 56.6787 blast 1155 .21CA194640 -T sua 2502 50 81 46 004 bat 1 sugarcanegbI57 2can 96.2732 87.0056 blast 1156 .21CA103332_T sua 2503 50 84 919 497 bat 1e sugarcanelgbI57 sugarcan 93.7888 74.2574 blast 1157 .21CA167616_T sugar 2504 50 82 199 257 1I 82 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. sugarcanelgbl57 sugarcan 68.5344 1158 .21CA103740_T s 2505 50 90 100 828 blastp 1 sugarcanelgb15 7 ca 1159 .21CA184547T sugar 2506 50 82 9725155 77.4834 tblastn 1 1160 sunflowerlgbl621 sunflowe 2507 50 87 98.1366 87.5 blastp CF089373_TI r 46 1161 wheatlgbl641CA wheat 2508 50 89 96.8944 65.9574 blastp 484201_TI 099 468 1162 apricotlgbl57.21 apricot 2509 51 86 100 65.9574 blastp CV049856_TI 468 arabidopsislgbl6 arabidop 32.6315 1163 51AT2G37180_T sis 2510 51 89 100 789 blastp 1 arabidopsislgbl6 arabidop 32.1799 1164 51AT2G39010_T sis 2511 51 92 100 308 blastp 1 b-juncealgbl641 86.9158 1165 EVGN00130608 b-juncea 2512 51 87 100 879 blastp 921231_TI b-juncealgbl641 90.3225 86.5979 1166 EVGN00605203 b-juncea 2513 51 86 806 381 blastp 140273_TI b-juncealgbl641 56.9892 91.3793 1167 EVGN21262514 b-juncea 2514 51 86 473 103 blastp 941904_TI b-juncealgbl641 85.3211 1168 EVGN00733314 b-juncea 2515 51 86 100 009 blastp 152324_TI b-juncealgbl641 89.2473 49.4047 1169 EVGN00041211 b-juncea 2516 51 86 118 619 blastp 340240_TI 1170 bjuncealgb164| b-juncea 2517 51 91 100 80.1724 blastp DT317704_TI 138 b-juncealgbl641 89.2473 79.8076 1171 EVGN00208014 b-juncea 2518 51 85 118 923 blastp 701957_TI b-juncealgbl641 36.3281 1172 EVGN00550314 b-juncea 2519 51 90 100 25 blastp 491066_TI b-juncealgbl641 90.3225 95.4545 1173 EVGN01267508 b-juncea 2520 51 90 806 455 blastp 262672_TI b-juncealgbl641 51.3812 1174 EVGN01803715 b-juncea 2521 51 89 100 155 blastp 320789_TI b-juncealgbl641 34.4019 1175 EVGN00397011 b-juncea 2522 51 91 100 729 tblastn 681539_TI 1176 bjrapalgbl62|D b rapa 2523 51 88 100 69.4029 blastp N965016_TI - 851 1177 bjrapalgb162|E b rapa 2524 51 87 100 32.5174 blastp X025548 TI 825 1178 bjapalgbi62B b rapa 2525 51 92 100 32.2916 blastp IG543764T Ti - III 1 1667,I 83 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 1179 bananalgbl601D banana 2526 51 89 100 27.8721 tblastn 119 N238638_TI baaa 26 51 8 10 279 1180 bananalgbl601D banana 2527 51 89 100 30.5921 tblastn 110 N238638_T2 bnn 2571 89 00 053 1181 barleylgbl57.31B barley 2528 51 87 100 32.7464 blastp E421292_TI 789 1182 barleylgbl57.31B barley 2529 51 83 100 64.5833 blastp J446923_TI 333 1183 beetgbl621BQ4 beet 2530 51 81 100 26.6862 blastp 88455_TI 17 1184 beetlgbl62|BVU beet 2531 51 84 100 32.2916 blast 60147_Ti 667 1185 canolalgbl6I1CX canola 2532 51 87 100 32.5174 blastp 189721_TI 825 1186 canolalgbl61ICB canola 2533 51 92 100 32.2916 blastp 686155_TI 667 1187 canolagbl6I1D canola 2534 51 87 100 325174 blast 1188 canolaIgbI61|ES canola 2535 51 86 96.7741 87.3786 blast 986486_TI 935 408 1189 canola 16 iEV canola 2536 51 82 100 3890108 tblastn 1190 caN7a4v053_T1D cassava 2537 51 93 100 62.8378 blast 1191 cas4491b6_T1D cassava 2538 51 94 100 688888 blastp 1192 cassavalgbl641B cassava 2539 51 81 100 32.8621 blastp 112 M259717_TI csaa 23 1 8 0 908 1193 castorbeanlgb16 castorbea 2540 51 81 100 46.5 blastp 01EE257493_T2 n 1194 castorbeanlgb16 castorbea 2541 51 81 100 34.4444 blastp 01EE257493_TI n 444 1195 cichoriumlgbI61 cichoriu 2542 51 94 100 80.8695 blastp IEH706421_Ti m 652 1196 cichoriumlgbI61 cichoriu 2543 51 90 100 32.5554 tblastn IEH708948_Ti m 259 1197 cichoriumlgbI61 cichoriu 2544 51 90 100 24.6684 tblastn IEH692078_TI m 35 1198 citrus4b68_TllC citrus 2545 51 82 100 86.1111 blastp 1199 cits Igb 57211 citrus 2546 51 90 100 27.5691 tblastn 1200 cloverlgbl621BB clover 2547 51 91 100 32.6315 blastp 120 903718_TI clvr 2451 9 10 789 1201 cloverlgbl621BB clover 2548 51 88 100 32.4041 blast 930902_TI 812 1202 cloverlgbl621BB clover 2549 51 91 92.4731 80.3738 blastp 911526_TI 183 318 1203 cloverlgbl621BB clover 2550 51 90 96.7741 81.0810 blastp 913405_TI 935 811 1204 cottongbl64ES cotton 2551 51 86 100 58.4905 blastp 804497_T1 66 1205 cottonlgb1641EX cotton 2552 51 89 90.3225 41.5156 tblastn 172153_TI c 2 5 806 507 84 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 1206 cowpealgb66|F cowpea 2553 51 89 90.3225 80 blastp F384339_TI copa 2551 8 806 8 1207 cowpealgbI66|F cowpea 2554 51 82 97.8494 88.3495 blastp F396241_TI 624 146 1208 cowpealgb66|E cowpea 2555 51 88 100 3284041 blastp 1209 coG857474_Ti1F cowpea 2556 51 89 100 68.8888 blastp cryptomerialgbl cryptome 78.8135 1210 661BY902595_T ria 2557 51 81 100 593 blastp 1 1211 cryptomerialgbl cryptome 2558 51 83 100 31.7406 blastp 661BJ937695_TI ria 143 cryptomerialgbl rpoe8.12 635 1212 661BW993227T rme 2559 51 82 88.1720 86.3157 blastp 1 1213 dandelionlgbI611 dandelio 2560 51 87 91.3978 75.2212 blastp DY802675_TI n 495 389 1214 fescuelgbI61IDT fescue 2561 51 82 84.9462 85.8695 blast 674412_TI 366 652 1215 fescuelgbI61IDT fescue 2562 51 84 100 76.8595 blastp 695652_TI 041 1216 fescuelgbI61IDT fescue 2563 51 83 95.6989 96.7391 blastp 702501_TI 247 304 1217 fesc8gb61 DT fescue 2564 51 83 100 8503211 blastp 1218 fesc8gbl6 DT fescue 2565 51 92 100 81.5789 blast 1219 gingergbI64|DY ginger 2566 51 87 100 32.9787 blastp 358169_TI 234 1220 gingergbI64|DY ginger 2567 51 82 93.5483 87 blast 366672_TI 871 1221 gingerlgbl643DY ginger 2568 51 91 100 27.7888 tblastn 1222 grapelgbI60IAFI grape 2569 51 82 100 32.5174 blastp 88843_T5 825 1223 ipomoealgbI57.2 ipomoea 2570 51 88 100 32.4041 blastp IBJ556470_TI 812 1224 01T57.21 lettuce 2571 51 90 100 32.6315 blastp DW158018_TI78 1225 lettuce gb157.21 lettuce 2572 51 90 100 34.7014 blastp DW091407_TI92 1226 lettuce Igbl157.21 lettuce 2573 51 89 89.2473 32.6771 blastp DW060777_TI 118 654 1227 lotuslgbI57.21A lotus 2574 51 83 100 88.5714 blast V775277_TI 286 1228 lotuslgbl57.21AI lotus 2575 51 84 100 32.4041 blastp 967387_Ti 812 1229 lotuslgbI57.21A lotus 2576 51 81 100 88.5714 blastp V774377_TI 286 1230 lotusIgbI57.21BP lotus 2577 51 80 73.1182 85 blastp 059122_Ti 796 1231 lotuslgbl57.21B1 lotu 81 94.6236 88 blastp 419853 TI 559 85 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 1232 lotuslgbl57.21BP lotus 2579 51 81 100 76.2295 blastp 049219_TI 082 1233 lotuslgbl57.21A lotus 2580 51 82 84.9462 86.8131 blastp V775053_TI 366 868 1234 lotuslgbl57.21A lotus 2581 51 85 95.6989 80.9090 blast V775249_TI 247 909 1235 maizeIgbl641AF maize 2582 51 88 100 32.4041 blast 326496_TI 812 1236 maizeIgbl641AY maize 2583 51 84 100 32.8621 blast 107589_TI 908 1237 medicagogbl57. medicag 2584 51 87 100 32.4041 blastp 21AI974409_TI o 812 1238 medicagolgbl57. medicag 2585 51 89 100 32.6315 blastp 21AI974231_TI o 789 1239 melonIgbl65|EB melon 2586 51 86 100 23.4848 tblastn 715587_TI 485 1240 oatIgb164ICN81 oat 2587 51 82 100 84.5454 blast 6056_TI 545 1241 oil-palmgb1661 oil-palm 2588 51 89 100 32.9787 blastp CN601069_TI 234 1242 oilpalmgb1661 oil-palm 2589 51 84 100 32.9787 blastp EL686181 TI 234 1243 peanutlgbl61 I peanut 2590 51 82 100 31.8493 blastp D037823_TI 151 1244 peanutlgbl6llC peanut 2591 51 88 100 32.1799 blastp D038014_TI 308 1245 periwinklelgbl6 periwink 2592 51 87 100 65.9574 blast 41AM232518_T2 le 468 physcomitrellalg physcom 33.3333 1246 b1571BI436955 itrella 2593 51 89 100 333 blastp TI physcomitrellalg physcom 32.5174 1247 b1571BJ198543 ie 2594 51 82 100 blastp TI itrella 254 5 2 10 825 physcomitrellalg physcom 33.2142 1248 b1571AW476973 itrella 2595 51 90 100 857 blastp T3 physcomitrellalg physcom 32.5259 1249 b571BJ962015 2596 51 82 100 516 blastp T1 1250 pinegbI57.2IA pine 2597 51 82 100 34.7014 blastp W870138_TI 925 1251 pinelgb157.21AI8 pine 2598 51 87 100 33.0960 blastp 13147_TI 854 1252 pinelgbI57.21AA pine 2599 51 87 100 33.0960 blastp 739836_TI 854 1253 pinelgbI57.21AL pine 2600 51 88 100 33.0960 blastp 750425_TI 854 1254 pinegbI57.21BG pine 2601 51 80 100 32.8621 blastp 038984_TI 908 1255 pinelgbI57.21A pine 2602 51 80 100 34.1911 blastp W225939_TI ____ 765 1256 pinelgbI57.21BQ pine 2603 51 81 100 32.8621 blastp 696500 TI 908 86 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 1257 pinelgbI57.21AL pine 2604 51 87 100 33.3333 blastp 751198_TI 333 1258 pinelgbl57.21BQ pine 2605 51 82 100 32.8621 blastp 695693_TI 908 1259 pinelgb157.21BF pine 2606 51 80 100 60.3896 blastp 517326_TI 104 1260 pinelgbl57.21AA pine 2607 51 81 100 34.7014 blastp 739625_TI 925 1261 pinelgb157.21BG pine 2608 51 82 100 32.8621 blastp 317873_TI 908 1262 pinelgbl57.21AI8 pine 2609 51 87 100 32.9787 blast 13147_T2 234 1263 pinelgb157.2|CF pine 2610 51 87 100 33.3333 blastp 388120_TI 333 1264 pinelgb157.21BE pine 2611 51 81 100 35.7692 blastp 662590_TI 308 1265 pinelgbI57.21BG pine 2612 51 82 100 32.8621 blast 318657_TI 908 1266 pinegbI57.21AA pine 2613 51 88 100 33.3333 blastp 557104_TI 333 1267 pinelgbI57.21A pine 2614 51 82 100 32.8621 blastp W870138 T2 908 1268 pinegbI57.21A pine 2615 51 82 100 32.8621 blastp W289749_TI 908 129 pinelgb157.211-7 pn 266 588 10 32.7464 bat 5016_TI 789 1270 pinegbI57.21AA pine 2617 51 80 100 35.0943 blastp 740005_TI 396 1271 pinegbI57.2|CA pine 2618 51 91 100 75.6097 blastp 305579_TI 561 1272 pinegbI57.21BE pine 2619 51 83 100 32.8621 blastp 187350_TI 908 1273 pinegbI57.2|CF pine 2620 51 84 100 32.9787 blastp 473539_TI 234 1274 pinegbI57.21A pine 2621 51 83 100 32.7464 blastp W290370_TI 789 1275 pinelgbI57.21A pine 2622 51 82 75.2688 88.6075 blastp W290691_TI 172 949 1276 pinelgbI57.21BG pine 2623 51 82 100 34.7014 blastp 318695_TI 925 1277 pineapplelgbI57. pineappi 2624 51 91 98.9247 78.6324 blast 21DT339628_TI e 312 786 1278 b157 21A poplar 2625 51 86 100 24.3455 tblastn 1279 8argb5721B poplar 2626 51 87 100 22.6094 tblastn U817536_T4 ppa 266 587 10 003 1280 potatogb157.21B potato 2627 51 80 98.9247 36.6533 blastp Q515617_T1 312 865 1281 potato lgb57.21B potato 2628 51 90 100 41.5178 blastp E920139_T2 571 1282 radishlgbI64|EV radish 2629 51 87 100 32.5174 blastp 526963_TI 825 1283 radishlgbI64|EV radish 2630 51 84 100 46.0396 blastp 567230_T2 , I _II_1_04 87 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 1284 radishlgbl64|EV radish 2631 51 92 100 32.2916 blastp 551004_TI 667 1285 radishlgbl64|EX radish 2632 51 86 100 48.1865 blastp 756464_TI 285 1286 radishlgbl64|EY radish 2633 51 91 100 31.9587 blast 910551_TI 629 1287 radishlgbl64|EW radish 2634 51 84 98.9247 87.6190 blastp 730466_TI 312 476 1288 radishlgbl641AF radish 2635 51 81 87.0967 48.6 tblastn 051128_TI 742 radishlgbl64lEX No4028 1289 r h64|EX radish predicted 51 86 100 40.0286 tblastn Protein 1290 ricelgbl57.21BE2 rice 2636 51 90 100 32.9787 blast 29418_TI 234 1291 ricelgbl57.21BE2 rice 2637 51 90 100 38.5892 blastp 29418_T3 116 1292 ricelgbl57.21AK rice 2638 51 91 100 68.8888 blast 107700_TI 889 1293 ricelgbl57.21BE2 rice 2639 51 90 100 54.0697 blastp 29418_T4 674 1294 ricelgbl57.21NM rice 2640 51 92 100 41.7040 blast 001066078_TI 359 1295 ricelgb157.21AW rice 2641 51 83 100 32.0689 blast 155505_TI 655 1296 ricelgbl57.21AW rice 2642 51 83 98.9247 35.7976 blastp 155505_T2 312 654 1297 ricelgbl57.21AK rice 2643 51 83 100 22.6461 tblastn 106746_TI 039 sorghumgbl61. 33.3333 1298 xenolAY107589 sorghum 2644 51 86 100 333 blastp TI sorghumlgbl61. 32.5174 1299 xenolA1947598_ sorghum 2645 51 92 100 825 blastp TI sorghumlgbl61. 31.4189 1300 xenoIAI855413_ sorghum 2646 51 80 100 189 blastp TI sorghumgbl61. 31.1418 1301 xenolAW565915 sorghum 2647 51 81 100 685 blastp TI sorghumlgbl61. 79.6610 1302 xenolCF481617_ sorghum 2648 51 90 100 169 blastp TI 1303 soybeanlgbl66|B soybean 2649 51 89 100 32.5174 blastp U549322_TI 825 1304 soybeanlgbl66|B soybean 2650 51 88 100 32.4041 blastp E352729_TI 812 1305 soybeanlgb1661B soybean 2651 51 91 100 71.5384 blastp 1974981_TI 615 1306 soybeanlgbl66|B soybean 2652 51 89 100 32.6315 blastp E658685_TI ____ 789 1307 soybeangbl66|B soybean 2653 51 81 100 20.5904 tblastn 1308 spikemosslgbl65 spikemos 2654 51 88 100 32.0689 blastp IDN838269 TI 2 655 88 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 1309 spikemosslgb165 spikemos 2655 51 88 100 32.0689 blastp IFE434019_TI s 655 1310 sprucelgb1621CO spruce 2656 51 89 100 32.8621 blast 225164_TI 908 1311 sprucelgb1621CO spruce 2657 51 81 100 33.8181 blast 258147_TI 818 1312 sprucelgb1621CO spruce 2658 51 88 100 46.0396 blast 230791_TI 04 1313 sprucelgbl62|DR spruce 2659 51 82 100 32.5 blastp 1314 sprucelgbl62|DR spruce 2660 51 81 100 34.1911 blast 560237_TI 765 1315 spurgelgb6D spurge 2661 51 83 100 86.1111 blastp V116550_TI III sugarcanelgbl57 sugarcan 88.1720 81.1881 1316 .2CA088464_T s 2662 51 87 43 188 blast 1 e sugarcanelgbl57 sugarcan 62.4161 1317 .2CA086583_T s 2663 51 92 100 074 blast 1 sugarcanelgbl57 sugarcan 62.3655 1318 .21CA234165_T e 2664 51 86 914 100 blast 1 sugarcane1gbl57 sugarcan 32.5174 1319 .2CA107998_T s 2665 51 89 100 825 blastp 1 sugarcanelgb157 sugarcan 34.1911 1320 .2CA204327_T s 2666 51 95 100 765 tblastn sugarcanelgb157 suacan8601 1.40 1321 .21CA067786 T sugar 2667 51 92 86.0215 18.6480 tblastn 1 1322 sunflowerlgb1621 sunflowe 2668 51 81 100 31.9587 blastp DY944685_TI r 629 1323 sunflowerlgbl621 sunflowe 2669 51 87 52.6881 100 blastp BQ968590_T1 r 72 1324 sunflowerlgb1621 sunflowe 2670 51 89 98.9247 41.3173 tblastn CD848850_TI r 312 653 1325 tobaccolgbl621E tobacco 2671 51 90 100 32.4041 blast B445876_TI 812 bat 1326 tobaccolgb1621E tobacco 2672 51 88 100 32.4041 blastp 136 B445188_TI tbco 27 1 8 0 812 1327 tobaccolgb621C tobacco 2673 51 81 100 34.7014 blastp 137 K720586_TI tbco 27 1 8 0 925 1328 tomatolgbl641C tomato 2674 51 92 88.1720 82.8282 blast 0750818_TI 43 828 1329 tomatolgbl641AI tomato 2675 51 81 98.9247 33.6996 blastp 772191_TI 312 337 1330 tomatolgb64A tomato 2676 51 89 100 39.2405 tblastn 130 W219533_T2 tmt 266 589 10 063 1331 triphysarialgbl6 triphysar 2677 51 88 95.6989 80.9090 blastp 41DR173305_TI ia 247 909 1332 triphysarialgbl6 triphysar 2678 51 90 100 75.6097 blastp 41EX984185_TI ia 561 1333 triphysarialgbl6 triphysar 2679 51 91 100 67.8832 blastp 41DR174019_Ti ia 1 117,I 89 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 1334 wheat~gbI641CA wheat 2680 51 94 100 67.3913 blastp 134 609068_TI wet 28 1 9 0 043 1335 wheat~gbl641BE wheat 2681 51 86 100 32.4041 blastp 430411_TI 812 1336 wheat~gb1641CK wheat 2682 51 80 100 30 blastp 208980_TI 1337 wheat~gbl641CA wheat 2683 51 96 95.6989 87.2549 blastp 620158_TI 247 02 1338 wheat~gbl641BE wheat 2684 51 88 100 32.7464 blastp 405395_TI 789 1339 wheat~gbl641CA wheat 2685 51 80 77.4193 93.5064 blast 647310_TI 548 935 1340 wheat~gbl641BE wheat 2686 51 87 100 32.7464 blastp 492099_TI 789 1341 wheat~gbl641BE wheat 2687 51 87 100 32.7464 blastp 402029_TI 789 1342 wheat~gbl641CA wheat 2688 51 86 88.1720 73.2142 blast 618130_TI 43 857 1343 wheat~gbl641CJ6 wheat 2689 51 82 100 69.4029 blastp 05707_TI 851 1344 wheat~gbl641CA wheat 2690 51 98 59.1397 98.2142 blastp 614209_Ti 849 857 1345 wheat~gbl641CA wheat 2691 51 82 100 77.5 blastp 701714_TI 1346 wheat~gbl641BE wheat 2692 51 93 96.7741 82.5688 blast 403921_TI 935 073 1347 wheat|gb1641BE wheat 2693 51 83 100 31.9587 blastp 137 217049_TI wet 29 1 8 0 629 1348 wheat gbI641CA wheat 2694 51 84 100 30.0322 tblastn 138 602649_TI wet 29 1 8 0 928 1349 wheat~gbl641CA wheat 2695 51 82 96.7741 33.3759 tblastn 486220_TI 935 591 b-juncealgb1641 64.3835 99.2957 1350 EVGN00044413 b-juncea 2696 52 85 616 746 blastp 933329_TI b-juncealgb1641 53.4246 99.1525 1351 EVGN00137910 b-juncea 2697 52 90 575 424 blastp 990746_TI 1352 barleylgb157.31B barley 2698 52 81 96.8036 73.4482 blast E413268_Ti 53 759 1353 barleylgb157.31A barley 2699 52 81 96.8036 73.4482 blastp J433979_TI 53 759 1354 barleylgbl57.31B barley 2700 52 82 96.8036 74.1258 blast E412979_Ti 53 741 brachypodiumig brachypo 96.8036 73.6111 1355 b16I.xeno1AFI3 dim 2701 52 83 53 11 blastp 9815_TI 1356 citruslgb157.21C citrus 2702 52 84 50.2283 100 blast X052950_TI 105 1357 cloverlgbl621BB clover 2703 52 93 52.5114 99.1379 blastp 913131_TI 155 31 1358 cloverlgbl621BB clover 2704 52 82 63.9269 99.2957 blastp 918704_Ti 406 746 1359 cottonlgb1641C0 cotton 2705 52 91 56.6210 93.9393 blast 103246 TI 046 939 90 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. 1360 cowpealgbl66|F cowpea 2706 52 83 51.1415 99.1150 blastp G883860_TI 525 442 1361 fescuelgbI61IDT fescue 2707 52 96 57.0776 93.2835 blastp 702489_TI 256 821 1362 fescuelgbI61IDT fescue 2708 52 96 57.0776 96.8992 blast 702846_TI 256 248 1363 ipomoealgbl57.2 ipomoea 2709 52 81 87.2146 74.7035 blastp IBU691146_TI 119 573 1364 maizelgbl641AI9 maize 2710 52 80 96.8036 73.6111 blastp 39909_TI 53 111 1365 maizelgbl641AF maize 2711 52 83 96.8036 74.3859 blast 130975_TI 53 649 1366 maizelgbl641AI9 maize 2712 52 83 96.8036 73.6111 blastp 47831_TI 53 111 1367 oil-palmgb1661 oil-palm 2713 52 80 96.8036 75.1773 blastp EL692065_TI 53 05 physcomirellalg pycm93.6073 73.4767 1368 b157IAW476973 2714 52 80 059 025 blastp -T1 physcomitrellaig physcm 96.3470 73.0103 bat 1369 b157113894596 _ e 2715 52 80 3 0 blast Ti physcomitrellaig physcom 93.6073 73.4767 bat 1370 b157IAW476973 2716 52 80 059 7 6 blast T2 1371 pinelgb157.21CF pine 2717 52 88 51.1415 99.1150 blastp 387570_TI 525 442 1372 radishgbl641EY radish 2718 52 88 54.3378 99.1666 blastp 904434_T2 995 667 1373 radishlgbl641FD radish 2719 52 84 63.0136 99.2805 blastp 960377_TI 986 755 1374 ricelgbl57.21AU rice 2720 52 81 96.8036 74.1258 blast 093957_TI 53 741 1375 ricelgb157.21BE0 rice 2721 52 92 57.0776 97.6562 blastp 39992_T2 256 5 1376 ricelgb157.21BE5 rice 2722 52 80 96.8036 73.1034 blast 30955_TI 53 483 1377 ryelgb1641BE58 rye 2723 52 82 61.1872 97.8102 blast 6469_TI 146 19 sorghumgbl61. 96.8036 72.6027 1378 xenolAW922622 sorghum 2724 52 83 53 397 blastp TI sorghumlgb161. 85.3881 1379 xenolBE344582_ sorghum 2725 52 80 279 74.8 blastp TI sorghumlgb161. 96.8036 73.3564 1380 xenoIAI855280_ sorghum 2726 52 82 53 014 blastp TI 1381 spikemosslgbl65 spikemos 2727 52 83 91.7808 71.5302 blastp IDN838148_TI 2 219 491 1382 spikemosslgbl65 spikemos 2728 52 83 91.7808 71.5302 blastp IDN838057_TI 2 219 491 sugarcanelgb157 sugarcan 90.4109 71.8978 blast 1383 .21CA139573 T sugar 2729 52 82 589 102 1 e 91 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. sugarcanelgbI57 sugarcan 57.0776 99.2063 blast 1384 .21CA145403_T sua 2730 52 90 256 492 bat 1 1385 sunflowerlgbI621 sunflowe 2731 52 80 57.0776 93.2835 blastp CF083179_TI r 256 821 1386 sunflowerlgbI621 sunflowe 2732 52 86 56.6210 89.2086 blastp BU035823_TI r 046 331 1387 switchgrasslgbI6 switchgr 2733 52 83 61.6438 99.2647 blastp 51DN140790_TI ass 356 059 1388 switchgrasslgbl6 switchgr 2734 52 81 66.2100 96.6666 blastp 51FE631354_TI ass 457 667 1389 tobaccolgbl62|D tobacco 2735 52 81 87.6712 48.7722 tblastn V159802_TI 329 269 1390 wheatIgbl641AF wheat 2736 52 82 96.8036 74.1258 blastp 139815_TI 53 741 1391 wheatIgbl641BE wheat 2737 52 81 96.8036 73.4482 blastp 406301_TI 53 759 1392 wheatIgbI641BE wheat 2738 52 81 96.8036 73.4482 blast 404904_TI 53 759 1393 wheatIgbI641BE wheat 2739 52 81 96.8036 73.4482 blastp 400219_TI 53 759 1394 wheatIgbI641CA wheat 2740 52 81 54.7945 90.1515 blastp 619093_TI 205 152 1395 wheatIgbI641BE wheat 2741 52 88 56.6210 93.2330 blast 605056_TI 046 827 1396 wheatlgbl641BQ wheat 2742 52 82 96.8036 74.1258 blastp 245211_TI 53 741 1397 wheatIgbI641BE wheat 2743 52 81 96.8036 73.4482 blastp 497487_TI 53 759 1398 wheat|gbI64IBQ wheat 2744 52 89 64.8401 100 blastp 295206_TI 826 1399 wheatIgbI641BE wheat 2745 52 80 98.6301 74.8275 blast 499954_TI 37 862 1400 wheatIgbI641BE wheat 2746 52 81 96.8036 73.4482 blastp 405794_TI 53 759 tomatolgbl641BP 5 881534_TI tomato 31 32 82 100 100 blastp barleylgbl57.31A 98.3935 98.7951 21 L501410_TI barley 47 46 87 743 8072 blastp antirrhinumgb 16 antirrhin 2844 61AJ559427_TI um 3052 25 88 100 100 blastp antirrhinumgb 16 antirrhin 2845 61AJ791214_TI um 3053 25 85 100 100 blastp bruguieralgbI661 bruguier 61.2903 2846 BP939664_TI a 3054 25 82 2258 100 blastp centaureagbl661 centaure 98.7903 2847 EL931601_TI a 3055 25 84 2258 100 blastp eucalyptusgbl6 eucalypt 98.7903 98.7903 2848 61CD668425_TI us 3056 25 85 2258 2258 blastp kiwilgb166|FG4 2849 09998_TI kiwi 3057 25 85 100 100 blastp kiwilgb166|FG4 2850 53166_TI kiwi 3058 25 86 100 100 blastp kiwilgb166|FG4 99.5967 2851 01585 TI kiwi 3059 25 87 7419 100 blastp 92 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. kiwilgbl66|FG4 2852 19790_TI kiwi 3060 25 85 100 100 blastp liriodendrongbl 661FD495170_T liriodend 99.1935 98.7951 2853 1 ron 3061 25 82 4839 8072 blastp poppyIgbl66|FG 82.6612 99.5145 2854 607362_TI POPPY 3062 25 82 9032 6311 blastp soybeanlgbl671A 2855 A660186_TI soybean 3063 25 83 100 100 blastp soybeangbl671C 95.5645 2856 A990807_TI soybean 3064 25 82 1613 100 blastp walnutsgbl66|C 2857 V196664_TI walnuts 3065 25 83 100 100 blastp antirrhinumgb 16 antirrhin 2858 61X70417_TI um 3066 26 90 100 100 blastp bananalgbl67|FF 99.4871 2859 560721_TI banana 3067 26 86 77.6 7949 blastp centaureagbl661 centaure 2860 EL932548_TI a 3068 26 86 100 100 blastp antirrhinumlgbl6 antirrhin 90.9090 2861 61AJ789802_TI um 3069 27 86 9091 100 blastp bruguieralgbI661 bruguier 69.9604 2862 BP938735_TI a 3070 27 81 7431 100 blastp eucalyptuslgbI6 eucalypt 90.9090 2863 61ES589574_TI us 3071 27 82 9091 100 blastp kiwilgb166|FG4 50.1976 99.2187 2864 27735_TI kiwi 3072 27 86 2846 5 blastp kiwilgb166|FG4 54.1501 2865 06415_TI kiwi 3073 27 81 9763 100 blastp kiwilgb166|FG4 99.2094 99.6031 2866 06885_TI kiwi 3074 27 84 8617 746 blastp liriodendrongbl 661CK744430_T liriodend 99.2094 99.6031 2867 1 ron 3075 27 81 8617 746 blastp poppyIgbI66|FG 83.0039 99.5260 2868 608493_TI poppy 3076 27 84 5257 6635 blastp soybeangbl671E 60.0790 96.2025 2869 V269611_TI soybean 3077 27 86 5138 3165 blastp amborellalgbl661 amborell 2870 CD482678_TI a 3078 28 82 100 100 blastp cenchruslgb1661 2871 BM084541_TI cenchrus 3079 28 81 100 100 blastp leymusIgbI66|E 2872 G376267_TI leymus 3080 28 80 100 100 blastp leymusgb1661E 2873 G386149_TI leymus 3081 28 80 100 100 blastp walnutslgbl661E 94.4954 2874 L895384_TI walnuts 3082 28 83 82 1284 blastp amborellalgbl661 amborell 98.2638 98.9547 2875 CD481950_TI a 3083 30 88 8889 0383 blastp antirrhinumgb 16 antirrhin 93.4027 2876 61AJ796874_TI um 3084 30 86 7778 100 blastp antirrhinumgb 16 antirrhin 73.2638 99.5260 2877 61AJ798039 TI um 3085 30 84 8889 6635 blastp 93 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. antirrhinumgb 16 antirrhin 85.0694 2878 61AJ792331_TI um 3086 30 88 4444 100 blastp antirrhinumgb 16 antirrhin 98.2638 99.3006 2879 61AJ558770_TI um 3087 30 86 8889 993 blastp bananalgbI67|FF 98.2638 98.9510 2880 558844_TI banana 3088 30 88 8889 4895 blastp beanIgbI67|CA8 98.2638 99.3031 2881 98412_TI bean 3089 30 81 8889 3589 blastp bruguieralgbI661 bruguier 52.7777 2882 BP941115_TI a 3090 30 86 7778 95 blastp bruguieralgbl661 bruguier 97.9166 99.3031 2883 BP939033_TI a 3091 30 85 6667 3589 blastp cenchruslgbI661 98.2638 98.9583 2884 EB656428_TI cenchrus 3092 30 85 8889 3333 blastp centaureagbl661 centaure 98.2638 98.9547 2885 EH767475_TI a 3093 30 86 8889 0383 blastp centaureagbl661 centaure 98.2638 98.9583 2886 EL935569_TI a 3094 30 86 8889 3333 blastp cycaslgbl66|CB 98.2638 98.9547 2887 089724_TI cycas 3095 30 84 8889 0383 blastp cycaslgbl66|CB 98.2638 98.2698 2888 088798_TI cycas 3096 30 83 8889 9619 blastp eucalyptuslgbI6 eucalypt 98.2638 99.3055 2889 61CD668044_TI us 3097 30 84 8889 5556 blastp eucalyptusgbl6 61AW191311_T eucalypt 98.2638 98.9547 2890 1 us 3098 30 87 8889 0383 blastp kiwilgb166|FG4 98.2638 99.3006 2891 03284_TI kiwi 3099 30 85 8889 993 blastp kiwilgb166|FG4 98.2638 99.3006 2892 04130_TI kiwi 3100 30 86 8889 993 blastp kiwilgb166|FG3 98.2638 98.9510 2893 96354_TI kiwi 3101 30 90 8889 4895 blastp kiwilgb166|FG4 72.2222 99.5215 2894 04890_TI kiwi 3102 30 85 2222 311 blastp kiwilgb166|FG4 62.1527 99.4444 2895 17962_TI kiwi 3103 30 87 7778 4444 blastp kiwilgb166|FG4 98.2638 96.9178 2896 03188_TI kiwi 3104 30 89 8889 0822 blastp kiwilgb166|FG4 68.0555 99.4923 2897 03647_TI kiwi 3105 30 85 5556 8579 blastp kiwilgb166|FG3 98.2638 99.3006 2898 97405_TI kiwi 3106 30 85 8889 993 blastp leymusgb1661E 99.3055 99.6551 2899 G384635_TI leymus 3107 30 85 5556 7241 blastp leymusgb1661C 98.2638 98.9726 2900 N466016_TI leymus 3108 30 83 8889 0274 blastp leymuslgb1661C 98.2638 98.9726 2901 N466006_TI leymus 3109 30 83 8889 0274 blastp leymusgb1661E 98.2638 98.9726 2902 G376500_TI leymus 3110 30 83 8889 0274 blastp liriodendrongbl 661CK749885_T liriodend 98.2638 98.9547 2903 1 ron 3111 30 89 8889 0383 blastp 94 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. liriodendrongbl 661CV002697_T liriodend 98.2638 98.9547 2904 1 ron 3112 30 88 8889 0383 blastp lovegrasslgbl671 98.2638 98.9619 2905 DN480914_TI lovegrass 3113 30 85 8889 3772 blastp nupharlgbI66|C 98.2638 98.9473 2906 D472824_TI nuphar 3114 30 86 8889 6842 blastp nupharlgbI66|C 98.2638 98.9473 2907 D472574_TI nuphar 3115 30 86 8889 6842 blastp nupharlgbI661C 51.7361 98.0263 2908 D473614_TI nuphar 3116 30 84 1111 1579 blastp peanutgbl671ES 64.2361 2909 759056_TI peanut 3117 30 83 1111 100 blastp poppyIgbI66IFE 98.6111 98.6206 2910 966578_TI poppy 3118 30 83 1111 8966 blastp pseudoroegnerial gbl671FF344096 pseudoro 98.2638 98.9726 2911 _TI egneria 3119 30 82 8889 0274 blastp pseudoroegnerial gbl671FF346975 pseudoro 98.6111 98.6254 2912 _TI egneria 3120 30 85 1111 2955 blastp pseudoroegnerial gbl671FF342094 pseudoro 98.2638 98.9726 2913 _TI egneria 3121 30 83 8889 0274 blastp soybeangbl67IC 94.4444 95.0877 2914 A898412_TI soybean 3122 30 84 4444 193 blastp switchgrasslgbl6 switchgr 52.4305 98.6928 2915 71FE621985_TI ass 3123 30 87 5556 1046 blastp switchgrasslgbl6 switchgr 98.2638 98.9583 2916 71DN141371_TI ass 3124 30 86 8889 3333 blastp tamarixlgbI66|C 78.4722 99.1228 2917 F199714_TI tamarix 3125 30 88 2222 0702 blastp tamarixlgbI66|C 98.2638 99.3031 2918 F226851_TI tamarix 3126 30 85 8889 3589 blastp walnutslgbl66|C 98.2638 99.3103 2919 B303847_TI walnuts 3127 30 84 8889 4483 blastp walnutslgbl66|C 98.2638 99.3031 2920 V194951_TI walnuts 3128 30 86 8889 3589 blastp walnutslgbl66|C 98.2638 99.3150 2921 V196162_TI walnuts 3129 30 85 8889 6849 blastp zamiaIgbI66|FD 98.2638 98.9547 2922 765004_TI zamia 3130 30 84 8889 0383 blastp antirrhinumlgbl6 antirrhin 98.7854 2923 6|AJ799752_TI um 3131 31 80 251 100 blastp kiwilgb166|FG4 74.0890 2924 00670_TI kiwi 3132 31 82 6883 100 blastp kiwilgb166|FG4 98.7854 98.3870 2925 18275_TI kiwi 3133 31 83 251 9677 blastp centaurealgbl661 centaure 88.5714 32.7402 2926 EL931588_TI a 3134 34 83 2857 1352 blastp soybeanlgbI671A 94.2857 36.2637 2927 W119586_TI soybean 3135 34 83 1429 3626 blastp soybeanlgbI671A 94.2857 36.6666 2928 W573764 TI soybean 3136 34 83 1429 6667 blastp 95 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. amborellalgbl661 amborell 65.7627 2929 FD440187_TI a 3137 35 81 1186 100 blastp centaurealgbl661 centaure 97.6271 96.6101 2930 EL931433_TI a 3138 35 82 1864 6949 blastp eucalyptuslgbI6 eucalypt 73.8983 2931 61CD668486_TI us 3139 35 85 0508 100 blastp petunialgbI66|D 2932 C243166_TI petunia 3140 36 86 100 100 blastp amborellalgbI661 amborell 99.6415 98.9399 2933 CD482946_TI a 3141 39 82 7706 2933 blastp antirrhinumgb 16 antirrhin 99.2831 98.5765 2934 61AJ559435_TI um 3142 39 88 5412 1246 blastp antirrhinumlgbl6 antirrhin 71.3261 2935 61AJ793990_TI um 3143 39 89 6487 100 blastp antirrhinumlgbl6 antirrhin 61.2903 87.2448 2936 61AJ559760_TI um 3144 39 83 2258 9796 blastp antirrhinumlgbl6 antirrhin 88.5304 2937 61AJ558545_TI um 3145 39 88 6595 100 blastp beangb167|FE6 2938 82762_TI bean 3146 39 86 100 100 blastp beanIgbI67|CV5 99.6415 98.9473 2939 31088_TI bean 3147 39 86 7706 6842 blastp beanIgbI67|CA9 99.6415 98.9473 2940 07460_TI bean 3148 39 86 7706 6842 blastp cenchruslgb1661 97.4910 95.8620 2941 EB655519_TI cenchrus 3149 39 83 3943 6897 blastp centaurealgbl661 centaure 53.7634 96.1538 2942 EL934360_TI a 3150 39 88 4086 4615 blastp centaurealgbl661 centaure 88.5304 2943 EH751120_TI a 3151 39 80 6595 100 blastp centaurealgbl661 centaure 83.5125 2944 EH752971_TI a 3152 39 87 448 100 blastp centaurealgbl661 centaure 91.7562 99.6168 2945 EH768434_TI a 3153 39 80 724 5824 blastp centaurealgbl661 centaure 83.1541 2946 EH767287_TI a 3154 39 83 2186 100 blastp cichoriumgbl66 cichoriu 89.9641 2947 IDT212008_TI m 3155 39 82 5771 100 blastp cichoriumgbl66 cichoriu 86.7383 97.6190 2948 IEL354583_TI m 3156 39 84 5125 4762 blastp eucalyptusgbl6 eucalypt 83.8709 2949 61CD668553_TI us 3157 39 87 6774 100 blastp eucalyptuslgbI6 eucalypt 69.8924 2950 61CD668534_TI us 3158 39 80 7312 100 blastp eucalyptusgbl6 eucalypt 99.3006 2951 61CD668523_TI us 3159 39 88 100 993 blastp eucalyptusgbl6 eucalypt 2952 61CD669942_TI us 3160 39 83 100 100 blastp kiwilgb166|FG4 99.2932 2953 05216_TI kiwi 3161 39 84 100 8622 blastp kiwilgb166|FG4 50.1792 2954 95821_TI kiwi 3162 39 86 1147 100 blastp kiwilgb166|FG4 64.5161 2955 17997 TI kiwi 3163 39 86 2903 100 blastp 96 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. kiwilgbl66|FG4 73.1182 2956 03725_TI kiwi 3164 39 83 7957 100 blastp kiwilgbl66|FG3 2957 97310_TI kiwi 3165 39 88 100 100 blastp kiwilgb166|FG4 99.3031 2958 08531_TI kiwi 3166 39 83 100 3589 blastp leymusgb166|E 55.5555 97.4683 2959 G381236_TI leymus 3167 39 81 5556 5443 blastp leymusgb166|E 96.7741 96.5517 2960 G376087_TI leymus 3168 39 80 9355 2414 blastp leymusIgb166|C 2961 D808804_TI leymus 3169 39 81 100 100 blastp leymusIgb166|E 51.2544 2962 G378918_TI leymus 3170 39 86 8029 100 blastp liriodendrongbl 661CK761396_T liriodend 99.6415 98.9619 2963 1 ron 3171 39 81 7706 3772 blastp nupharlgbI66|ES 61.2903 98.2758 2964 730700_TI nuphar 3172 39 81 2258 6207 blastp poppyIgbI66IFE 88.5304 2965 965621_TI poppy 3173 39 84 6595 100 blastp pseudoroegnerial gb1671FF340233 pseudoro 2966 _TI egneria 3174 39 81 100 100 blastp switchgrasslgbl6 switchgr 2967 71FE638368_TI ass 3175 39 80 100 100 blastp switchgrasslgbl6 switchgr 99.2831 98.9583 2968 71FE657460_TI ass 3176 39 80 5412 3333 blastp switchgrasslgbI6 switchgr 78.8530 86.5900 2969 71FE641178_TI ass 3177 39 82 4659 3831 blastp switchgrasslgbl6 switchgr 94.2652 95.7295 2970 71FE619224_TI ass 3178 39 82 3297 3737 blastp switchgrassgbl6 switchgr 2971 71FE657460_T2 ass 3179 39 81 100 100 blastp tamarixlgbI66|E 56.2724 98.7421 2972 H051524_TI tamarix 3180 39 82 0143 3836 blastp walnutslgbl66|C 99.3031 2973 B304207_TI walnuts 3181 39 84 100 3589 blastp walnutsgbl66|C 2974 B303561_TI walnuts 3182 39 84 100 100 blastp walnutslgbl661E 89.6057 98.8235 2975 L892579_TI walnuts 3183 39 84 3477 2941 blastp zamialgbl66|DY 96.0573 94.3262 2976 032141_TI zamia 3184 39 80 4767 4113 blastp zamiaIgbI66|FD 99.6415 98.9247 2977 764669_TI zamia 3185 39 80 7706 3118 blastp zamialgbl66|DY 94.6236 92.2535 2978 034152_TI zamia 3186 39 80 5591 2113 blastp antirrhinumgb 16 antirrhin 2979 61AJ568195_TI um 3187 40 87 100 100 blastp antirrhinumgb 16 antirrhin 2980 61AJ568110_TI um 3188 40 87 100 100 blastp bananalgbl67|FL 83.0388 92.4901 2981 657842_TI banana 3189 40 81 6926 1858 blastp 97 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Custer name m SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. bruguieralgbl661 bruguier 2982 EF126757_TI a 3190 40 84 100 100 blastp centaurealgbl661 centaure 92.5795 2983 EH765776_TI a 3191 40 84 053 100 blastp eucalyptusgbl6 eucalypt 2984 61AJ627837_TI us 3192 40 85 100 100 blastp kiwilgb166|FG4 2985 11924_TI kiwi 3193 40 86 100 100 blastp kiwilgb166|FG4 2986 00706_TI kiwi 3194 40 85 100 100 blastp kiwilgb166|FG4 71.0247 2987 18898_TI kiwi 3195 40 84 3498 98.5 blastp kiwilgb166|FG4 72.7915 2988 20187_TI kiwi 3196 40 85 1943 100 blastp kiwilgb166|FG3 2989 98010_TI kiwi 3197 40 86 100 100 blastp petuniagbI66|A 2990 F452012_TI petunia 3198 40 88 100 100 blastp tamarixgbI66|C 2991 V121772_TI tamarix 3199 40 83 100 100 blastp walnutsgbl661E 2992 L893208_TI walnuts 3200 40 84 100 100 blastp beanIgbI67|FD7 58.6092 2993 86218_TI bean 3201 41 83 7152 100 blastp citrusgbI66|CX 72.5165 99.5412 2994 074333_T2 citrus 3202 41 81 5629 844 blastp citruslgbI66|CX 96.0264 91.4285 2995 074333_TI citrus 3203 41 83 9007 7143 blastp kiwilgb166|FG4 87.0860 97.0479 2996 20468_T2 kiwi 3204 41 84 9272 7048 blastp kiwilgb166|FG4 58.9403 95.6989 2997 20468_TI kiwi 3205 41 83 9735 2473 blastp tamarixlgbI66|E 60.9756 95.2380 2998 G972096_TI tamarix 3206 42 82 0976 9524 blastp pseudoroegnerial gbl671FF353501 pseudoro 98.3935 98.7951 2999 _TI egneria 3207 43 86 743 8072 blastp switchgrassgbl6 switchgr 98.7951 99.1967 3000 71FE646459_TI ass 3208 43 86 8072 8715 blastp switchgrasslgbI6 switchgr 71.4859 93.2291 3001 71FL887003_TI ass 3209 43 84 4378 6667 blastp wheatgbl641BE 98.3935 98.7951 3002 403397_TI wheat 3210 43 86 743 8072 blastp leymusgb1661E 52.0161 3003 G381168_TI leymus 3211 44 96 2903 100 blastp pseudoroegnerial gb1671FF341567 pseudoro 3004 _TI egneria 3212 44 97 100 100 blastp switchgrasslgbl6 switchgr 3005 71FE618822_TI ass 3213 44 91 100 100 blastp switchgrasslgbI6 switchgr 78.2258 3006 71FE633786_TI ass 3214 44 92 0645 100 blastp eucalyptuslgbI6 eucalypt 97.5155 60.7843 3007 61CB967586 TI us 3215 46 84 2795 1373 blastp 98 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. liriodendrongbl 661CK762443_T liriodend 74.5341 3008 1 ron 3216 46 80 6149 100 blastp switchgrasslgbl6 switchgr 61.3899 3009 71FE615387_TI ass 3217 46 90 100 6139 blastp walnutslgbl661E 97.5155 55.4744 3010 L893973_TI walnuts 3218 46 82 2795 5255 blastp beanIgbI67|FD7 88.1720 79.6116 3011 82805_TI bean 3219 47 91 4301 5049 blastp beanIgbI671EY4 38.5892 3012 57935_TI bean 3220 47 82 100 1162 blastp cichoriumgbl66 cichoriu 67.3913 3013 IEH685648_T2 m 3221 47 80 100 0435 blastp cichoriumgbl66 cichoriu 32.1799 3014 IEH685648_TI m 3222 47 80 100 308 blastp citruslgbI66|CK 76.8595 3015 701147_TI citrus 3223 47 90 100 0413 blastp citruslgbI66|CX 79.5698 88.0952 3016 544905_TI citrus 3224 47 81 9247 381 blastp cycaslgbl66|CB 87.0967 30.6818 3017 088978_TI cycas 3225 47 82 7419 1818 blastp cycasIgbI66|EX 76.2295 3018 920749_TI cycas 3226 47 88 100 082 blastp kiwilgb166|FG4 98.9247 77.9661 3019 29765_TI kiwi 3227 47 84 3118 0169 blastp leymusgb1661C 79.4871 3020 N466394_TI leymus 3228 47 87 100 7949 blastp leymusgb1661E 32.7464 3021 G376019_TI leymus 3229 47 88 100 7887 blastp leymuslgb1661E 31.9587 3022 G390723_TI leymus 3230 47 81 100 6289 blastp leymusgb1661E 32.1799 3023 G387193_TI leymus 3231 47 81 100 308 blastp nupharlgbI661C 75.6097 3024 D473277_TI nuphar 3232 47 91 100 561 blastp nupharlgbI66|F 96.7741 81.0810 3025 D384794_TI nuphar 3233 47 86 9355 8108 blastp nupharlgbI66|C 69.9248 3026 D475538_TI nuphar 3234 47 89 100 1203 blastp nupharlgbI66|C 48.9473 3027 D472711_TI nuphar 3235 47 91 100 6842 blastp petuniagbI66|D 58.0645 98.1818 3028 C240378_TI petunia 3236 47 92 1613 1818 blastp pseudoroegnerial gb1671FF344283 pseudoro 32.7464 3029 _TI egneria 3237 47 87 100 7887 blastp sorghumgbI6I.c 32.5174 3030 rpIAI724931_T1 sorghum 3238 47 92 100 8252 blastp switchgrasslgbl6 switchgr 33.6956 3031 71FL923354_TI ass 3239 47 82 100 5217 blastp switchgrassgbl6 switchgr 98.9247 74.7967 3032 71FE657461_TI ass 3240 47 92 3118 4797 blastp switchgrasslgbl6 switchgr 72.2222 3033 71FL765830 TI ass 3241 47 82 100 2222 blastp 99 Polynuc. Organs Polypep. Hom. of % Query Algorith SEQ ID Cluster name mn SEQ ID SEQ ID Ident. cover. Subject m NO: NO: NO: cover. switchgrasslgbl6 switchgr 97.8494 3034 71FL915169_TI ass 3242 47 83 6237 87.5 blastp tamarixlgbl66|E 65.6470 3035 H054604_TI tamarix 3243 47 83 100 5882 tblastn walnutsgb166|C 80.1724 3036 B303798_TI walnuts 3244 47 91 100 1379 blastp bruguieralgbl661 bruguier 52.0547 3037 BP942548_TI a 3245 48 82 9452 100 blastp bruguieralgbl661 bruguier 56.6210 91.1764 3038 BP938825_TI a 3246 48 89 0457 7059 blastp centaurealgbl661 centaure 87.2146 3039 EH739326_TI a 3247 48 83 1187 50 tblastn eucalyptuslgbl6 eucalypt 56.1643 91.7910 3040 61CD669176_TI us 3248 48 87 8356 4478 blastp leymus1gb1661E 52.0547 90.4761 3041 G382428_TI leymus 3249 48 80 9452 9048 blastp liriodendronlgbl 661CK743477_T liriodend 94.0639 54.5454 3042 1 ron 3250 48 81 2694 5455 tblastn marchantialgb16 marchant 94.0639 72.5352 3043 61BJ840587_TI ia 3251 48 83 2694 1127 blastp marchantialgbl6 marchant 93.1506 71.5789 3044 61C96070_TI ia 3252 48 83 8493 4737 blastp nupharlgbl661C 65.7534 99.3103 3045 K748374_TI nuphar 3253 48 82 2466 4483 blastp pseudoroegnerial gbl671FF340047 pseudoro 96.8036 73.4482 3046 _T1 egneria 3254 48 81 5297 7586 blastp pseudoroegnerial gbl671FF340899 pseudoro 96.8036 73.4482 3047 _T1 egneria 3255 48 81 5297 7586 blastp pseudoroegnerial gbl671FF352644 pseudoro 96.8036 74.1258 3048 _T1 egneria 3256 48 82 5297 7413 blastp sorghumgbl61.c rpISBGWPO301 96.8036 74.1258 3049 88_TI sorghum 3257 48 80 5297 7413 blastp switchgrasslgb16 switchgr 96.8036 74.1258 3050 71FL718379_TI ass 3258 48 81 5297 7413 blastp switchgrasslgb16 switchgr 96.8036 73.1034 3051 71FE639195 TI ass 3259 48 82 5297 4828 blastp Table 3: Homologues and orthologues of the AQP proteins are provided. Homology was calculated as % of identity over the aligned sequences. Polynuc. = Polynucleotide; Polypep. = Polypeptide; Hom. = Homologues/Orthologues; % Ident. = percent identity; Cover. = coverage. 5 EXAMPLE 2 mRNA EXPRESSION OF IN-SILICO EXPRESSED POLYNUCLEOTIDES Messenger RNA levels were determined using reverse transcription assay followed by quantitative Real-Time PCR (qRT-PCR) analysis. RNA levels were compared between leaves 10 of 20 days old seedlings of tomato plants grown under salinity water. A correlation analysis 100 between mRNA levels in different experimental conditions/genetic backgrounds was performed in order to determine the role of the gene in the plant. Materials and Experimental Methods Quantitative Real Time RT-PCR (qRT-PCR) - To verify the level of expression, 5 specificity and trait-association, Reverse Transcription followed by quantitative Real-Time PCR (qRTPCR) was performed on total RNA extracted from leaves of 2 tomato varieties namely YO361 (salt tolerant variety) and FA191 (salt sensitive variety). Messenger RNA (mRNA) levels were determined for AQP genes, expressed under normal and stressed conditions. Twenty days-old tomato seedlings were grown in soil and soaked with 300 mM NaCl for LO 0, 1, 6, 24, 118 hours. Leaves were harvested and frozen in liquid nitrogen and then kept at -80 'C until RNA extraction. Total RNA was extracted from leaves using RNeasy plant mini kit (Qiagen, Hilden, Germany) and by using the protocol provided by the manufacturer. Reverse transcription was performed using 1 tg total RNA, using 200 U Super Script II Reverse Transcriptase enzyme (Invitrogen), 150 ng random deoxynucleotide hexamers (Invitrogen), 500 L5 [tM deoxynucleotide tri-phosphates (dNTPs) mix (Takara, Japan), 0.2 volume of x5 reverse transcriptase (RT) buffer (Invitrogen), 0.01 M dithiothreitol (DTT), 40 U RNAsin (Promega), diethylpyrocarbonate (DEPC) treated double distilled water (DDW) was added up to 24 [tl. Mix of RNA, random deoxynucleotide hexamers, dNTPs mix and DEPC treated DDW was incubated at 65 'C for 5 minutes, followed by 4 'C for 5 minutes. Mix of reverse !0 transcriptase (RT) buffer, dithiothreitol (DTT) and RNAsin was added to the RT reactions followed by incubation at 25 'C for 10 minutes and at 42 'C for 2 minutes afterwards. Finally, Super Script II Reverse Transcriptase enzyme was added to the RT reactions that were further incubated for 50 minutes at 42 'C, followed by 70 'C for 15 minutes. cDNA was diluted 1:20 in Tris EDTA, pH = 7.5 for MAB69 and housekeeping genes. 25 For MAB58 and MAB59 cDNA was diluted 1:2 due to very weak expression and consequently for housekeeping genes cDNA was diluted 1:8 in order to insert the Ct values in calibration curve range. 5 [tL of the diluted cDNA was used for qPCR. For qPCR amplification, primers of the AQP genes were designed, as summarized in Table 4 below. The expression level of the housekeeping genes: Actin (SEQ ID NO: 2841), 30 GAPDH (SEQ ID NO: 2842) and RPL19 (SEQ ID NO: 2747) was determined in order to normalize the expression level between the different tissues.

101 Table 4 Primers for qPCR amplification Forward Reverse Reverse primer sequence Gene prnmer Forward primer sequence primer SEQ ID (5'-*3') SEQ ID NO: NO: MAB58 2829 CTTTTGGTAGGGCCGATGA 2830 CGAAGATGAAGGTGG AG ATAAGAGCT 2831 CAGTATGAACGTCTCCGGT 2832 CAACAGCACCTAGCAA MAB59 GG CTGACC MAB69 2833 TGTCTTGGATTCCATTGAG 2834 GTTTGAGCTGCTGTCC CACT CCA Actin (SEQ 2835 CCACATGCCATTCTCCGTC 2836 GCTTTTCTTTCACGTCC ID NO: T CTGA 2841) GAPD H (SEQ 2837 TTGTTGTGGGTGTCAACGA 2838 ATGGCGTGGACAGTGG ID NO: GA TCA 2842) RPL19 (SEQ 2839 CACTCTGGATATGGTAAGC 2840 TTCTTGGACTCCCTGTA ID NO: GTAAGG CTTACGA 2747) 1 1 1 1 Table 4. 5 Experimental Results Changes in mRNA levels of AQP genes in leaves of plants under salt tolerance Steady state levels of tomato AQP genes in leaves of tolerant versus sensitive lines, under salinity conditions are summarized in Table 5 below. In all 3 cases, aquaporin gene expression was increased after plant was exposed to salt stress. Gene peak expression was higher in the salt 10 tolerance tomato line (Y0361) versus the sensitive line (FA191). The elevated gene expression demonstrates the involvement of the tested AQP genes in tomato plants tolerating high salinity.

102 Table 5 Expression levels of tomato AQP genes Well name (cDNA) MAB58 MAB59 MAB69* leaf FA191 0 h 2.86 5.97 875 leaf FA191 1 h 4.56 5.73 597 leaf FA191 6 h 5.34 8.2 945 leaf FA191 24 h 42.7 62.4 613 leaf FA191 118 h 55.4 22.2 1800 leaf Y0361 0 h 1.96 2.81 638 leaf Y0361 1 h 2.33 0.517 583 leaf Y0361 6 h 2.88 5.66 464 leaf Y0361 24 h 75.4 139 513 leaf Y0361 118 h 26.3 Not determined 2300 Table 5: Provided are the steady state levels of tomato AQP genes under salinity conditions [the incubation periods in the salt solution are provided in hours (h)]. Different dilutions of cDNA were 5 used (1:20 for MAB69 and 1:2 for MAB58 and MAB59). Numbers are given after normalization for each sample. EXAMPLE 3 GENE CLONING AND GENERATION OF BINARY VECTORS FOR PLANT 10 EXPRESSION To validate their role in improving ABST and yield, the AQP genes were over-expressed in plants, as follows. Cloning strategy Selected genes from those presented in Example 1 were cloned into binary vectors for 15 the generation of transgenic plants. For cloning, the full-length open reading frames (ORFs) were identified. EST clusters and in some cases mRNA sequences were analyzed to identify the entire open reading frame by comparing the results of several translation algorithms to known proteins from other plant species. In case where the entire coding sequence is not found, RACE kits from Ambion or Clontech (RACE = Rapid Access to cDNA Ends) were used to prepare 20 RNA from the plant samples to thereby access the full cDNA transcript of the gene. In order to clone the full-length cDNAs, Reverse Transcription followed by PCR (RT PCR) was performed on total RNA extracted from leaves, roots or other plant tissues, growing under either normal or nutrient deficient conditions. Total RNA extraction, production of cDNA and PCR amplification was performed using standard protocols described elsewhere 25 (Sambrook J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Manual., 2nd Ed. Cold Spring Harbor Laboratory Press, New York.), and are basic for those skilled in the art. PCR products were purified using PCR purification kit (Qiagen) and sequencing of the amplified PCR products was performed, using ABI 377 sequencer (Applied Biosystems).

103 Usually, 2 sets of primers were ordered for the amplification of each gene, via nested PCR (meaning first amplifying the gene using external primers and then using the produced PCR product as a template for a second PCR reaction, where the internal set of primers are used). Alternatively, one or two of the internal primers were used for gene amplification, both in 5 the first and the second PCR reactions (meaning only 2-3 primers were designed for a gene). To facilitate further cloning of the cDNAs, a 8-12 bp extension is added to the 5' primer end of each internal primer. The primer extension includes an endonuclease restriction site. The restriction sites are selected using two parameters: (a) The restriction site does not exist in the cDNA sequence; and (b) The restriction sites in the forward and reverse primers are designed so the LO digested cDNA is inserted in the sense formation into the binary vector utilized for transformation. In Table 6 below, primers used for cloning tomato and barley AQPs are provided. Table 6 L5 Primers used for cloning tomato and barley AQP genes MLAB Forward external primer Forward internal Reverse external Reverse internal MAB Foeqaexernal pr--,ime primer sequence primer sequence primer sequence gene sequence fro -3 from 5'-->3' (SEQ from 5'--.3' (SEQ from 5'-v3' (SEQ ID ID NO:) ID NO:) NO:) GGAGTCGACGACCAT GGAGTCGACTT TGAGCTCACTTC TGAGCTCCCATCC 55 CAAGTTTTAAGTGAC AAGTACATTCTT AAAACCATCCG GTTGTCAAAATGA TTC (2770) TAGTGAGAGCC TTGTC (2772) AC (2773) TTC (770)(2771) GGAGTCGACGT CGAGCTCGTAA CGAGCTCAAGAC 56 AAGAAACAATA AGCCAAGTTTTG AAACAAAGAGAA ATGCCAATTTC AAAGAC (2775) GAGGG (2776) (2774) GTTAAAAATGC GCGATATCTAA GCGATATCAGCCG 57 CGATCAACC ATAACAAAAGC TCCGAATAAACAA (2777) CGTCCG (2778) AG (2779) AATGTCGACCGAATT TATGTCGACTTC TTTCTAGAGGTC TTTCTAGAGATGT 58 GATCTCCTTCTTGATC ATTTCTTGGGTC TGGGATTATCGT GCAGGCAGCTAC (2780) ACTCG (2781) CTTG (2782) ATAC (2783) AATGTCGACTTTAAG AATGTCGACTC AATCTAGATTAGA 59 CGGTGTGTTTTGTG ACAATTATGCA CCCAAACATACAA (2784) GCCACG (2785) ACTTCAC (2786)) TAAGTCGACACAAAC AATGTCGACCTT TGAGCTCTGGAGA 69 CTTATCCTGGTCTCAT GGATTCCATTGA AAGAAAACTTTAG C (2787) GCACTC (2788) ATACA (2789) AACTGCAGAGCTGTA AACTGCAGTGT TCCCGGGCCAG TCCCGGGCTTCAA 70 CATGGTCCTCCTCC ACATGGTCCTCC ACAAAACTTCA TTTCATCTTCTGA (2790) TCCG (2791) ATTTCATC (2792) TTTC (2793) AAAGTCGACGGAAAA TTTGTCGACCTT AATCTAGACAA AATCTAGAGTACT 71 TGCATTAAAACCTTA AGTTTTCTCCCA GTAGAGGTACT AGGTAGGGACAA AG (2794) CATATGG (2795) AGGTAGGGAC TATGATATG (2797) (2796) 104 MAB Forward external primer Forward internal Reverse external Reverse internal MAB Foeqaue ternal prime primer sequence primer sequence primer sequence gene sequence fro -3 from 5'--3' (SEQ from 5'->3' (SEQ from 5'->3' (SEQ ID ID NO:) ID NO:) NO:) AATGTCGACGTGGAG AATGTCGACCTC TATCTAGAGGATG 72 GAGGAGTCTTTGATA CAACACTCTTAT CAACTACAAAGA C (2798) CAATT CA AATTG (2800) TTCTGCAGGTTT TCCCGGGGCATAG 74 GGGAGTTATTG TTCACACAGAGCA ATCTAAGATG AATC (2802) (2801) AATGTCGACCTGTAT AATGTCGACGT TTTCTAGACTAG AATCTAGATTAGA 76 CCTCTTAAGTATGAA CGTCTTGTATGT TGGTATAGATC GCTGGAGAATGA TCG (2803) ATTTGTACTACT ATTTTATGGTGA ACTGAAGC (2806) G (2804) C (2805) AACTGCAGCTTCTTTC AACTGCAGCTTT ACCCGGGAATT TCCCGGGTCCAAC 77 ACCGAGTGGGAG CACCGAGTGGG TATGATTC TAGCTGTTATGAT (2807) AGAG (2808) 2809) TCTG (2810) AAGATATCAAAAAAA AAGATATCAAC AAGATATCGACCA 79 TGTCGAAGGACGTG AATGTCGAAGG CCAACTCTAGTCT (2811) ACGTGATTGA CATACC (2813) (2812) AGATTCGAATCTTTA AATCTAGAGAA AGAGCTCTTAAGG 115 GCCTG GTCACAGAGAA GAAATTCATCACA (2814) AACAGTCGAG CAAGG (2816) (2815) AAAGTCGACCTCATC TTTGTCGACCAT TATCTAGAATTG TTTCTAGAGACCG 116 AGTGTTAAAGCCATA AAGCCCTCTTTG AATCGAAAGGG TGACACACCATTT AG (2817) AGTGTG (2818) AAACAC (2819) GTAC (2820) TTTCTAGACTCA TGAGCTCCAGATA 117 GCGACAACATT GAGAAGCATGCA TCATCTC (2821) TCATC (2822) Table 6. PCR products were purified (PCR Purification Kit, Qiagen, Germany) and digested with the restriction endonucleases (Roche, Switzerland) according to the sites design in the primers 5 (Tables 8 and 9 below). Each of the digested PCR products was cloned first into high copy plasmid pBlue-script KS [Hypertext Transfer Protocol://World Wide Web (dot) stratagene (dot) com/manuals/212205 (dot) pdf] which was digested with the same restriction enzymes. In some cases (Table 8, below) the Nopaline Synthase (NOS) terminator originated from the binary vector pBI101.3 [nucleotide coordinates 4417-4693 in GenBank Accession No. U12640 (SEQ 10 ID NO:2824)] was already cloned into the pBlue-script KS, between the restriction endonuclease sites Sac and EcoRI, so the gene is introduced upstream of the terminator. In other cases (Table 9, below) the At6669 promoter (SEQ ID NO: 2823) is already cloned into the pBlue-script KS, so the gene is introduced downstream of the promoter. The digested PCR products and the linearized plasmid vector were ligated using T4 DNA ligase enzyme (Roche, 105 Switzerland). Sequencing of the inserted genes was conducted, using ABI 377 sequencer (Applied Biosystems). Sequences of few of the cloned AQP genes, as well as their encoded proteins are listed in Table 7, below. 5 Table 7: Cloned sequences Serial Gene Name Polynucleotide SEQ ID Polypeptide SEQ ID No NO: NO: 1 MAB115 2748 2765 2 MAB116 2749 47 3 MAB117 2750 48 4 MAB54 2751 27 5 MAB55 2752 28 6 MAB56 2753 29 7 MAB57 2754 30 8 MAB58 2755 2766 9 MAB59 2756 2767 10 MAB69 2757 33 11 MAB70 2758 34 12 MAB71 2759 2768 13 MAB72 2760 2769 MAB72 14 (Optimized for expression in Arabidopsis,Tomato 2843 2769 and Maize) 15 MAB74 2761 38 16 MAB76 2762 40 17 MAB77 2763 41 18 MAB79 2764 43 Table 7. The genes were digested again and ligated into pPI or pGI binary plasmids, harboring the At6669 promoter (between the HindIII and SalI restriction endonucleases site) (Table 8). In 10 other cases the At6669 promoter together with the gene are digested out of the pBlue-script KS plasmid and ligated into pPI or pGI binary plasmids, using restriction endonucleases as given in Table 8.

106 Table 8 Restriction enzyme sites used to clone the MAB AQP genes into pKS+NOS terminator high copy plasmid, followed by cloning into the binary vector pGI+At6669 promoter Restriction Restriction Restriction Restriction Restriction MAB enzymes used enzymes used enzymes used for enzymes used enzymes used gene No. for cloning for cloning into cloning into for cloning for digesting (SEQ ID into high copy high copy binary vector- into binary the binary NO:) plasmid- plasmid- FORWARD vector- vector FORWARD REVERSE REVERSE 54 (SEQ ID XbaI SacI SalI EcoRI SalI/EcoRI NO:275 1) 55 (SEQ ID SalI SacI SalI EcoRI SalI/EcoRI NO:2752) 56 (SEQ ID SalI SacI SalI EcoRI SalI/EcoRI NO:2753) 58 (SEQ ID SalI XbaI SalI EcoRI SalI/EcoRI NO:2755) 59 (SEQ ID SalI XbaI SalI EcoRI SalI/EcoRI NO:2756) 69 (SEQ ID SalI SacI SalI EcoRI SalI/EcoRI NO:2757) 71 (SEQ ID SalI XbaI SalI EcoRI SalI/EcoRI NO:2759) 72 (SEQ ID SalI XbaI SalI EcoRI SalI/EcoRI NO:2760) 76 (SEQ ID SalI XbaI SalI EcoRI SalI/EcoRI NO:2762) 115 (SEQ ID XbaI SacI SalI EcoRI SalI/EcoRI NO:2748) 116 (SEQ ID SalI XbaI SalI EcoRI SalI/EcoRI NO:2749) 117 (SEQ ID XbaI SacI SalI EcoRI SalI/EcoRI NO:2750)_ Table 8: MAB AQP genes cloned into pKS+NOS terminator high copy plasmid, followed by cloning into 5 the binary vector pGI+At6669 promoter.

107 Table 9 Restriction enzyme sites used to clone the MAB AQP genes into pKS+At6669 promoter high copy plasmid, followed by cloning promoter+gene into pGI binary vector Restriction Restriction Restriction Restriction Restriction MAB enzymes used enzymes used enzymes used for enzymes used enzymes used gene No. for cloning for cloning into cloning into for cloning for digesting (SEQ ID into high high copy binary vector- into binary the binary NO:) copy plasmid- plasmid- FORWARD vector- vector FORWARD REVERSE REVERSE 57 (SEQ ID Blunt EcoRV SalI EcoRV SalI /Ecl136 II NO:2754 70 (SEQ NOI758 PstI SmaI BamHI SmaI /Ecl136II ) /Ec113611 74 (SEQ NOI761 PstI SmaI BamHI SmaI /Ecl136II ) /Ec113611 77 (SEQ NOI763 PstI SmaI BamHI SmaI /Ecl136II 79 (SEQ NOI764 EcoRI EcoRV SalI SmaI SalI /Ecl136 II Table 9: MAB AQP genes cloned into pKS+At6669 promoter high copy plasmid, followed by cloning 5 promoter+gene into pGI binary vector. The pPI plasmid vector was constructed by inserting a synthetic poly-(A) signal sequence, originating from pGL3 basic plasmid vector (Promega, Acc. No. U47295; bp 4658 4811) into the HindlIl restriction site of the binary vector pBI101.3 (Clontech, Acc. No. 10 U12640). pGI (Figure 1) is similar to pPI, but the original gene in the back bone is GUS-Intron, rather than GUS. The cloned genes were sequenced. Synthetic sequences (such as of MAB54, nucleotide SEQ ID NO: 2751, which encodes protein SEQ ID NO: 27; or MAB72 SEQ ID NO:2843, which encodes SEQ ID NO:2769) of some of the cloned polynucleotides were ordered from a commercial supplier (GeneArt, 15 GmbH). To optimize the coding sequence, codon-usage Tables calculated from plant transcriptomes were used [example of such Tables can be found in the Codon Usage Database available online at Hypertext Transfer Protocol://World Wide Web (dot) kazusa (dot) or (dot) jp/codon/]. The optimized coding sequences were designed in a way that no changes were introduced in the encoded amino acid sequence while using codons preferred for expression in 20 dicotyledonous plants mainly tomato and Arabidopsis; and monocotyledonous plants such as maize. Such optimized sequences promote better translation rate and therefore higher protein 108 expression levels. To the optimized sequences flanking additional unique restriction enzymes sites were added to facilitate cloning genes in binary vectors. Promoters used: CaMV 35S promoter (SEQ ID NO: 2825) and Arabidopsis At6669 promoter (SEQ ID NO: 2823; which is SEQ ID NO:61 of W004081173 to Evogene Ltd.). 5 EXAMPLE 4 GENERATION OF TRANSGENIC PLANTS EXPRESSING THE AQP GENES Experimental Results Arabidopsis transformation- Arabidopsis transformation of the following MAB genes LO and orthologues: MAB 115, MAB54, MAB55, MAB56, MAB57, MAB58, MAB59 (ortholog of MAB58), MAB69, MAB70, MAB71, MAB72, MAB74, MAB76, MAB77, MAB79, MAB116 (ortholog of MAB115 and MAB55), and MAB117 (the sequence identifiers of the cloned polynucleotides and their expressed polypeptides are provided in Table 7 above) was performed according to Clough SJ, Bent AF. (1998) "Floral dip: a simplified method for Agrobacterium L5 mediated transformation of Arabidopsis thaliana." Plant J. 16(6): 735-43; and Desfeux C, Clough SJ, Bent AF. (2000) "Female reproductive tissues are the primary targets of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method." Plant Physiol. 123(3): 895-904; with minor modifications. Briefly, Arabidopsis thaliana Columbia (Col0) To Plants were sown in 250 ml pots filled with wet peat-based growth mix. The pots were covered !0 with aluminum foil and a plastic dome, kept at 4 'C for 3-4 days, then uncovered and incubated in a growth chamber at 18-24 'C under 16/8 hours light/dark cycles. The To plants were ready for transformation six days prior to anthesis. Single colonies of Agrobacterium carrying the binary vectors harboring the AQP genes are cultured in LB medium supplemented with kanamycin (50 mg/L) and gentamycin (50 mg/L). The cultures were incubated at 28 'C for 48 25 hours under vigorous shaking and centrifuged at 4000 rpm for 5 minutes. The pellets comprising Agrobacterium cells were resuspended in a transformation medium which contained half strength (2.15 g/L) Murashige-Skoog (Duchefa); 0.044 p.M benzylamino purine (Sigma); 112 pg/L B5 Gambourg vitamins (Sigma); 5 % sucrose; and 0.2 ml/L Silwet L-77 (OSI Specialists, CT) in double-distilled water, at pH of 5.7. 30 Transformation of To plants was performed by inverting each plant into an Agrobacterium suspension such that the flowering stem was submerged for 3-5 seconds. Each inoculated To plant was immediately placed in a plastic tray, then covered with clear plastic dome to maintain humidity and kept in the dark at room temperature for 18 hours to facilitate 109 infection and transformation. Transformed (transgenic) plants were then uncovered and transferred to a greenhouse for recovery and maturation. The transgenic To plants were grown in the greenhouse for 3-5 weeks until siliques maturation, and then seeds were harvested and kept at room temperature until sowing. 5 For generating T1 and T 2 transgenic plants harboring the genes, seeds collected from transgenic To plants are surface-sterilized by soaking in 70 % ethanol for 1 minute, followed by soaking in 5 % sodium hypochlorite and 0.05 % Triton X-100 for 5 minutes. The surface sterilized seeds are thoroughly washed in sterile distilled water then placed on culture plates containing half-strength Murashige-Skoog (Duchefa); 2 % sucrose; 0.8 % plant agar; 50 mM LO kanamycin; and 200 mM carbenicylin (Duchefa). The culture plates are incubated at 4 'C for 48 hours then transferred to a growth room at 25 'C for an additional week of incubation. Vital T 1 Arabidopsis plants are transferred to fresh culture plates for another week of incubation. Following incubation the T 1 plants are removed from culture plates and planted in growth mix contained in 250 ml pots. The transgenic plants were allowed to grow in a greenhouse to L5 maturity. Seeds harvested from T 1 plants are cultured and grown to maturity as T 2 plants under the same conditions as used for culturing and growing the T 1 plants. At least 10 independent transformation events are created from each construct for which T2 seeds are collected. The introduction of the gene is determined for each event by PCR performed on genomic DNA extracted from each event produced. !0 Transformation of tomato (Var M82) plants with putative cotton genes- Tomato (Solanum esculentum, var M82) transformation and cultivation of transgenic plants is effected according to: "Curtis I.S, Davey M.R, and Power J.B. 1995. "Leaf disk transformation". Methods Mol. Biol. 44, 59-70 and Meissner R, Chague V, Zhu Q, Emmanuel E, Elkind Y, Levy A.A. 2000. "Technical advance: a high throughput system for transposon tagging and promoter 25 trapping in tomato". Plant J. 22, 265-74; with slight modifications. EXAMPLE 5 EVALUATING TRANSGENIC ARABIDOPSIS PLANT GROWI'H UNDER ABIOTIC STRESS AS WELL AS UNDER FAVORABLE CONDITIONS IN TISSUE CULTURE 30 ASSAY Assay 1: plant growth under osmotic stress [poly (ethylene glycol) (PEG)] in tissue culture conditions - One of the consequences of drought is the induction of osmotic stress in the area surrounding the roots; therefore, in many scientific studies, PEG (e.g., 25 % PEG8000) is 110 used to simulate the osmotic stress conditions resembling the high osmolarity found during drought stress. Surface sterilized seeds were sown in basal media [50 % Murashige-Skoog medium (MS) supplemented with 0.8 % plant agar as solidifying agent] in the presence of Kanamycin 5 (for selecting only transgenic plants). After sowing, plates were transferred for 2-3 days for stratification at 4 'C and then grown at 25 'C under 12-hour light 12-hour dark daily cycles for 7 to 10 days. At this time point, seedlings randomly chosen were carefully transferred to plates containing 25 % PEG: 0.5 MS media or Normal growth conditions (0.5 MS media). Each plate contained 5 seedlings of the same transgenic event, and 3-4 different plates (replicates) for each LO event. For each polynucleotide of the invention at least four independent transformation events were analyzed from each construct. Plants expressing the polynucleotides of the invention were compared to the average measurement of the control plants (empty vector or GUS reporter gene under the same promoter) used in the same experiment. Digital imaging - A laboratory image acquisition system, which consists of a digital L5 reflex camera (Canon EOS 300D) attached with a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which included 4 light units (4x150 Watts light bulb) and located in a darkroom, was used for capturing images of plantlets sawn in agar plates. The image capturing process was repeated every 2-5 days starting at day 1 till day 10-15 (see for example the images in Figures 2A-B) !0 An image analysis system was used, which consists of a personal desktop computer (Intel P4 3.0 GHz processor) and a public domain program - ImageJ 1.39 (Java based image processing program which was developed at the U.S. National Institutes of Health and freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/). Images were captured in resolution of 10 Mega Pixels (3888 x 2592 pixels) and stored in a low 25 compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data was saved to text files and processed using the JMP statistical analysis software (SAS institute). Seedling analysis - Using the digital analysis seedling data was calculated, including leaf area, root coverage and root length. The relative growth rate for the various seedling parameters was calculated according to 30 the following formulas II, III and IV. Formula II: Relative growth rate of leaf area = (A rosette area / At) * (1/ rosette area ti) 111 A rosette area is the interval between the current rosette area (measured at t 2 ) and the rosette area measured at the previous day (Area ti) At is the time interval (t 2 -ti, in days) between the current analyzed image day (t 2 ) and the previous day (ti). 5 Thus, the relative growth rate of leaf area is in units of 1/day. Formula III: Relative growth rate of root coverage = (A root coverage area / At) * (1/ root coverage area ti) LO A root coverage area is the interval between the current root coverage area (measured at t 2 ) and the root coverage area measured at the previous day (Area ti) At is the time interval (t 2 -ti, in days) between the current analyzed image day (t 2 ) and the previous day (ti). Thus, the relative growth rate of root coverage area is in units of 1/day. L5 Formula IV: Relative growth rate of root length = (A root length / At) * (1/ root length ti) A root length is the interval between the current root length (measured at t 2 ) and the root length measured at the previous day (Area ti) 0 At is the time interval (t 2 -ti, in days) between the current analyzed image day (t 2 ) and the previous day (ti). Thus, the relative growth rate of root length is in units of 1/day. At the end of the experiment, plantlets were removed from the media and weighed for the determination of plant fresh weight. Plantlets were then dried for 24 hours at 60 'C, and 25 weighed again to measure plant dry weight for later statistical analysis. Growth rate was determined by comparing the leaf area coverage, root coverage and root length, between each couple of sequential photographs, and results were used to resolve the effect of the gene introduced on plant vigor, under osmotic stress, as well as under optimal conditions. Similarly, the effect of the gene introduced on biomass accumulation, under osmotic stress as well as under 30 optimal conditions, was determined by comparing the plants' fresh and dry weight to that of control plants (containing an empty vector or the GUS reporter gene under the same promoter). From every construct created, 3-5 independent transformation events were examined in replicates.

112 Statistical analyses - To identify genes conferring significantly improved tolerance to abiotic stresses or enlarged root architecture, the results obtained from the transgenic plants were compared to those obtained from control plants. To identify outperforming genes and constructs, results from the independent transformation events tested were analyzed separately. 5 To evaluate the effect of a gene event over a control the data was analyzed by Student's t-test and the p value was calculated. Results wer considered significant if p < 0.1. The JMP statistics software package was used (Version 5.2.1, SAS Institute Inc., Cary, NC, USA). Experimental Results - The polynucleotide sequences of the invention were assayed for a number of desired traits. LO Tables 10-14 depict analyses of the above mentioned growth parameters of seedlings overexpressing the polynucleotides of the invention under the regulation of the At6669 promoter (SEQ ID NO:2823) when grown under osmotic stress (25 % PEG) conditions. Table 10 L5 MAB70 -25 %PEG Event No. Control 7971.3 7972.1 7974.1 A P A P A P RGR of Roots Coverage between day 5 and 10 0.16 0.29 0.00 0.31 0.00 0.30 0.02 RGR of Roots Length between day 1 and 5 0.07 0.12 0.05 0.13 0.03 Table 10: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under 25 % PEG. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. !0 Table 11 MAB76 - 25 %PEG Event No. Control 7635.4 7635.1 A IP A I P RGR of Roots Coverage between day 5 and 10 0.16 0.22 0.02 0.21 0.09 Table 11: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under 25 % PEG. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. 25 Table 12 MAB79 - 25 %PEG Event No. Control 7324.1 7961.1 A P A P Dry Weight [gr] 0.01 0.01 0.06 Fresh Wight [gr] 0.08 0.13 0.07 Leaf Area on day 5 0.15 0.19 0.05 RGR of Roots Coverage between day 5 and 10 0.16 0.32 0.05 RGR of Roots Length between day 1 and 5 0.07 0.11 0.02 Table 12: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under 25 % PEG. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer 30 to days from planting.

113 Table 13 MAB56 - 25 %PEG Event No. Control 6802.10 A P Roots Coverage on day 7 2.46 3.38 0.09 Roots Length on day 7 2.81 3.43 0.07 Table 13: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under 25 % PEG. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer 5 to days from planting. Table 14 MAB58 - 25 %PEG Event No. Control 6783.30 A P Leaf Area on day 7 0.31 0.41 0.03 Leaf Area on day 14 0.80 1.09 0.02 Fresh Weight 0.18 0.31 0.00 Dry Weight [gr] 0.01 0.013 0.01 Table 14: Provided are the growth and biomass parameters of transgenic or control plants as measured in LO Tissue Calture growth under 25 % PEG. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Tables 15-29 depict analyses of the above mentioned growth parameters of seedlings overexpressing the polynucleotides of the invention under the regulation of the At6669 L5 promoter in Normal Growth conditions (0.5 MS medium). Table 15 MAB70 - Normal Growth Conditions Event No. Control 7971.3 7972.1 7974.1 7974.3 A P A P A P A P Dry Weight [gr] 0.01 0.01 0.03 0.01 0.05 0.02 0.04 0.01 0.07 Fresh Wight [gr] 0.14 0.24 0.04 0.22 0.10 Leaf Area on day 10 0.46 0.59 0.00 Leaf Area on day 5 0.21 0.30 0.10 RGR of Roots Coverage between 0.18 0.42 0.00 0.37 0.00 0.32 0.01 day 5 and 10 RGR of Roots Length between day 0.06 0.15 0.01 0.16 0.00 0.15 0.00 1 and 5 RGR of Roots Length between day 0.22 0.32 0.09 5 and 10 Table 15: Provided are the growth and biomass parameters of transgenic or control plants as measured in 20 Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

114 Table 16 MAB71 - Normal Growth Conditions Event No. Control 7331.5 7332.2 7333.5 A P A P A P Dry Weight [gr] 0.01 0.01 0.05 Fresh Wight [gr] 0.14 0.20 0.08 RGR of Roots Coverage between day 5 and 10 0.18 0.28 0.04 0.26 0.07 RGR of Roots Length between day l and 5 0.06 0.11 0.02 0.11 0.01 Table 16: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. 5 The indicated days refer to days from planting. Table 17 MAB74 - Normal Growth Conditions Event No. Control 7981.1 7982.4 7983.9 A P A P A P Dry Weight [gr] 0.01 0.01 0.09 0.02 0.05 0.01 0.06 Fresh Wight [gr] 0.14 0.21 0.01 Leaf Area on day 10 0.46 0.67 0.01 Leaf Area on day 5 0.21 0.29 0.04 0.29 0.01 0.27 0.00 RGR of Roots Coverage between day 5 and 10 0.18 0.31 0.07 RGR of Roots Coverage between day 1 and 5 0.73 1.70 0.07 RGR of Roots Length between day 1 and 5 0.06 0.12 0.09 0.14 0.03 RGR of Roots Length between day 5 and 10 0.22 0.45 0.05 0.58 0.00 Table 17: Provided are the growth and biomass parameters of transgenic or control plants as measured in LO Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 18 MAB76 - Normal Growth Conditions Event No. Control 7633.3 7635.4 7635.1 A P A P A P RGR Leaf Area between day 5 and 10 0.24 0.34 0.04 0.33 0.07 RGR of Roots Coverage between day 5 and 10 0.18 30.3800.08 RGR of Roots Length between day l and 5 0.06 0.13 0.02 15 Table 18: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 19 20 MAB77 - Normal Growth Conditions Event No. Control 7931.11 8211.2 8212.2 A P A P A P Dry Weight [gr] 0.01 0.01 0.08 0.01 0.10 Roots Coverage on day 10 2.77 4.33 0.07 RGR Leaf Area between day 5 and 10 0.24 0.38 0.10 0.35 0.04 RGR of Roots Coverage between day 5 and 10 0.18 0.27 0.09 0.29 0.09 RGR of Roots Length between day 1 and 5 0.06 0.10 0.03 Table 19: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. 25 115 Table 20 MAB79 - Normal Growth Conditions Event No. Control 7323.3 7324.1 7961.1 7962.2 A P A P A P A P Dry Weight [gr] 0.01 0.02 0.03 0.02 0.08 0.01 0.00 Fresh Wight [gr] 0.14 0.34 0.04 0.31 0.09 Leaf Area on day 10 0.46 0.92 0.01 0.90 0.01 0.81 0.00 Leaf Area on day 5 0.21 0.29 0.02 0.28 0.00 Roots Coverage on day 10 2.77 5.31 0.02 4.59 0.07 3.81 0.00 3.71 0.01 RGR Leaf Area between day 5 and 0.24 0.36 0.02 10 RGR Leaf Area between day l and 0.82 1.34 0.00 5 RGR of Roots Coverage between 0.18 0.45 0.06 0.39 0.02 day 5 and 10 RGR of Roots Length between day 0.06 0.12 0.02 0.17 0.06 0.14 0.00 1 and 5 Table 20: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. 5 The indicated days refer to days from planting. Table 21 MAB 15 - Normal Growth Conditions Event No. Control 8561.2.. 8564.1.. 8564.2.. 8565.1.. A P A P A P A P Dry Weight [gr] 0.005 0.006 0.00 0.009 0.00 Leaf Area on day 10 0.24 0.27 0.00 Leaf Area on day 5 0.35 0.38 0.01 Roots Coverage on day 10 1.67 2.13 0.02 Roots Coverage on day 5 3.38 4.80 0.03 Roots Length on day 10 2.39 2.74 0.00 Roots Length on day 5 3.46 4.22 0.02 RGR Leaf Area between day 5 0.37 0.43 0.00 0.48 0.00 and 10 RGR Leaf Area between day 1 0.16 0.19 0.00 and 5 RGR of Roots Coverage 1.89 3.21 0.00 2.24 0.02 2.23 0.02 3.47 0.01 between day 5 and 10 RGR of Roots Coverage 0.33 0.35 0.00 0.41 0.00 0.43 0.00 0.44 0.00 between day 1 and 511 111 RGR of Roots Length between 0.37 0.56 0.02 0.53 0.06 0.68 0.00 day 1 and 5 RGR of Roots Length between 0.15 0.18 0.00 0.17 0.00 0.18 0.00 day 5 and 10 Table 21: Provided are the growth and biomass parameters of transgenic or control plants as measured in 10 Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

116 Table 22 MAB54 - Normal Growth Conditions Event No. Control 8181.2.. 8182.2.. 8184.3.. 8185.3.. A P A P A P A P Dry Weight [gr] 0.005 0.009 0.00 0.008 0.00 0.008 0.00 0.007 0.00 Roots Coverage on day 10 1.67 1.80 0.04 Roots Coverage on day 5 3.38 4.27 0.02 3.40 0.00 Roots Length on day 10 2.39 2.52 0.01 Roots Length on day 5 3.46 4.11 0.02 3.50 0.00 RGR Leaf Area between day 5 0.37 0.45 0.00 and 10 RGR Leaf Area between day 1 0.16 0.17 0.04 0.19 0.00 and 5 RGR of Roots Coverage 1.89 3.59 0.00 3.60 0.01 2.28 0.01 2.20 0.01 between day 5 and 10 RGR of Roots Coverage 0.33 0.52 0.00 0.55 0.00 0.39 0.00 0.36 0.00 between day 1 and 5 RGR of Roots Length between 0.37 0.70 0.00 0.73 0.00 0.48 0.08 0.51 0.02 day 1 and 5 RGR of Roots Length between 0.15 0.21 0.01 0.22 0.01 0.17 0.00 0.17 0.00 dayS5 and_10 ______________________ Table 22: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. 5 The indicated days refer to days from planting. Table 23 MAB55 - Normal Growth Conditions Event No. 6802.1 6802.11 6802.8 6805.3 A P A P A P A P Roots Coverage on day 7 3.67 5.93 0.04 Roots Coverage on day 14 7.40 13.26 0.04 Roots Length on day 7 3.99 5.32 0.01 Roots Length on day 14 6.14 7.80 0.04 7.65 0.04 RGR of Roots Coverage between day 0.53 1.30 0.00 1.11 0.02 0.93 0.02 1 and 7 RGR of Roots Length between day 1 0.29 0.48 0.00 0.45 0.03 0.43 0.02 and 7 Fresh Weight 0.19 0.10 0.00 0.12 0.01 Dry Weight [gr] 0.01 0.01 0.00 0.01 0.00 Table 23: Provided are the growth and biomass parameters of transgenic or control plants as measured in 10 Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

117 Table 24 MAB56 - Normal Growth Conditions Event No. 6691.2 6691.3 6693.2 6693.5 A P A P A P A P Roots Coverage on day 7 3.67 5.81 0.00 5.67 0.01 Roots Length on day 7 3.99 6.21 0.00 5.39 0.00 Roots Length on day 14 6.14 8.32 0.01 8.01 0.02 RGR of Roots Length between day 7 0.09 0.05 0.01 0.05 0.06 0.06 0.08 and 14 Fresh Weight 0.19 0.14 0.03 0.14 0.03 Dry Weight [gr] 0.01 0.01 0.02 0.01 0.02 0.00 0.00 Table 24: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 25 MAB57 - Normal Growth Conditions Event No. 6912.14 6912.2 6912.6 6914.1 A P A P A P A P Roots Coverage on day 7 3.67 6.40 0.00 6.71 0.00 Roots Coverage on day 14 7.40 17.33 0.00 15.27 0.05 12.30 0.02 Roots Length on day 7 3.99 5.54 0.00 6.12 0.00 6.34 0.00 Roots Length on day 14 6.14 8.76 0.00 8.48 0.01 7.97 0.02 7.85 0.04 RGR of Roots Length between day 0.29 0.39 0.06 1 and 7 RGR of Roots Length between day 0.09 0.04 0.09 7 and 14 Fresh Weight 0.19 0.24 0.09 0.11 0.01 0.11 0.01 Dry Weight [gr] 0.01 0.01 0.01 0.01 0.01 Table 25: Provided are the growth and biomass parameters of transgenic or control plants as measured in LO Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 26 MAB58 - Normal Growth Conditions Event No. 6783.1 6783.2 6783.3 A P A P A P Roots Coverage on day 7 3.67 6.18 0.00 Roots Coverage on day 14 7.40 12.65 0.02 12.00 0.09 Roots Length on day 7 3.99 5.20 0.02 Roots Length on day 14 6.14 7.38 0.09 7.51 0.07 7.59 0.08 RGR of Roots Length between day 1 and 7 0.29 0.35 0.07 Fresh Weight 0.19 0.13 0.03 Dry Weight [gr] 0.01 0.01 0.00 15 Table 26: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

118 Table 27 MAB59 - Normal Growth Condition Event No. 6791.4 6793.4 6794.4 A P A P A P Roots Coverage on day 7 3.67 5.61 0.04 5.23 0.09 Roots Coverage on day 14 7.40 9.65 0.09 10.28 0.09 Roots Length on day 7 3.99 5.47 0.08 4.95 0.09 Roots Length on day 14 6.14 7.70 0.07 RGR of Roots Coverage between day 1 and 7 0.53 0.80 0.09 RGR of Roots Length between day 7 and 14 0.09 0.05 0.10 Fresh Weight 0.19 0.09 0.00 Dry Weight [gr] 0.01 0.004 0.00 0.005 0.02 Table 27: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. 5 The indicated days refer to days from planting. Table 28 MAB69 - Normal Growth Conditions Event No. 6651.1 6651.12 6651.13 6651.8 A P A P A P A P Roots Length on day 7 3.99 4.97 0.10 Roots Length on day 14 6.14 8.01 0.02 RGR of Roots Length between day 0.29 0.35 0.09 1 and 7 Fresh Weight 0.19 0.12 0.02 0.12 0.01 Dry Weight [gr] 0.01 0.007 0.09 0.005 0.00 0.006 0.00 Table 28: Provided are the growth and biomass parameters of transgenic or control plants as measured in LO Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 29 MAB72 - Normal Growth Conditions Event No. Control 8552.1.. 8552.4.. 8553.2.. A P A P A P Dry Weight [gr] 0.005 0.008 0.00 0.008 0.00 0.007 0.00 Leaf Area on day 10 0.24 0.24 0.01 Roots Coverage on day 10 1.67 1.84 0.01 1.93 0.02 1.89 0.03 Roots Coverage on day 5 3.38 3.60 0.04 3.90 0.06 4.50 0.00 Roots Length on day 10 2.39 2.48 0.01 Roots Length on day 5 3.46 3.70 0.04 3.65 0.04 3.84 0.00 RGR Leaf Area between day 5 and 10 0.37 0.47 0.00 0.39 0.00 RGR Leaf Area between day 1 and 5 0.16 0.20 0.09 RGR of Roots Coverage between day 5 and 10 1.89 1.93 0.03 2.29 0.06 3.04 0.01 RGR of Roots Coverage between day 1 and 5 0.33 0.35 0.00 0.52 0.00 RGR of Roots Length between day 1 and 5 0.37 0.55 0.01 RGR of Roots Length between day 5 and 10 0.15 0.16 0.00 0.18 0.00 0.22 0.01 15 Table 29: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under normal growth conditions. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

119 EXAMPLE 6 EVALUATING TRANSGENIC ARABIDOPSIS PLANT GROWTH UNDER ABIOTIC STRESS AS WELL AS FAVORABLE CONDITIONS IN GREENHOUSE ASSAY ABS tolerance: Yield and plant growth rate at high salinity concentration under 5 greenhouse conditions - This assay followed the rosette area growth of plants grown in the greenhouse as well as seed yield at high salinity irrigation. Seeds were sown in agar media supplemented only with a selection agent (Kanamycin) and Hoagland solution under nursery conditions. The T 2 transgenic seedlings were then transplanted to 1.7 trays filled with peat and perlite. The trays were irrigated with tap water (provided from the pots' bottom). Half of the LO plants were irrigated with a salt solution (40-80 mM NaCl and 5 mM CaCl 2 ) so as to induce salinity stress (stress conditions). The other half of the plants was irrigated with tap water (normal conditions). All plants were grown in the greenhouse until mature seeds, then harvested (the above ground tissue) and weighted (immediately or following drying in oven at 50 'C for 24 hours). High salinity conditions were achieved by irrigating with a solution L5 containing 40-80 mM NaCl ("ABS" growth conditions) and compared to regular growth conditions. Each construct was validated at its T2 generation. Transgenic plants transformed with a construct including the uidA reporter gene (GUS) under the AT6669 promoter or with an empty vector including the AT6669 promoter were used as control. !0 The plants were analyzed for their overall size, growth rate, flowering, seed yield, weight of 1,000 seeds, dry matter and harvest index (HI- seed yield/dry matter). Transgenic plants performance was compared to control plants grown in parallel under the same conditions. Mock- transgenic plants expressing the uidA reporter gene (GUS-Intron) or with no gene at all, under the same promoter were used as control. 25 The experiment was planned in nested randomized plot distribution. For each gene of the invention three to five independent transformation events were analyzed from each construct. Digital imaging - A laboratory image acquisition system, which consists of a digital reflex camera (Canon EOS 300D) attached with a 55 mm focal length lens (Canon EF-S series), mounted on a reproduction device (Kaiser RS), which included 4 light units (4 x 150 Watts 30 light bulb) was used for capturing images of plant samples. The image capturing process was repeated every 2 days starting from day 1 after transplanting till day 16. Same camera, placed in a custom made iron mount, was used for capturing images of larger plants sawn in white tubs in an environmental controlled 120 greenhouse. The tubs were square shape include 1.7 liter trays. During the capture process, the tubs were placed beneath the iron mount, while avoiding direct sun light and casting of shadows. An image analysis system was used, which consists of a personal desktop computer 5 (Intel P4 3.0 GHz processor) and a public domain program - ImageJ 1.39 (Java based image processing program which was developed at the U.S National Institutes of Health and freely available on the internet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/). Images were captured in resolution of 10 Mega Pixels (3888 x 2592 pixels) and stored in a low compression JPEG (Joint Photographic Experts Group standard) format. Next, analyzed data LO was saved to text files and processed using the JMP statistical analysis software (SAS institute). Leaf analysis - Using the digital analysis leaves data was calculated, including leaf number, area, perimeter, length and width. Vegetative growth rate: the relative growth rate (RGR) of leaf number and rosette area were calculated formulas V and VI, respectively. L5 Formula V: Relative growth rate of leaf number = (A leaf number / At) * (1/ leaf number ti) A leaf number is the interval between the current leaf number (measured at t 2 ) and the leaf number measured at the previous day (Area ti) At is the time interval (t 2 -ti, in days) between the current analyzed image day (t 2 ) and !0 the previous day (ti). Thus, the relative growth rate of leaf number is in units of 1/day. Formula VI: Relative growth rate of rosette area = (A rosette area / At) * (1/ rosette area ti) 25 A rosette area is the interval between the current rosette area (measured at t 2 ) and the rosette area measured at the previous day (Area ti) At is the time interval (t 2 -ti, in days) between the current analyzed image day (t 2 ) and the previous day (ti). Thus, the relative growth rate of rosette area is in units of 1/day. 30 Seeds average weight - At the end of the experiment all seeds were collected. The seeds were scattered on a glass tray and a picture was taken. Using the digital analysis, the number of seeds in each sample was calculated.

121 Dry weight and seed yield - On about day 80 from sowing, the plants were harvested and left to dry at 30 'C in a drying chamber. The biomass and seed weight of each plot were measured and divided by the number of plants in each plot. Dry weight = total weight of the vegetative portion above ground (excluding roots) after drying at 30 'C in a drying chamber; 5 Seed yield per plant = total seed weight per plant (gr). 1000 seed weight (the weight of 1000 seeds) (gr.) . Harvest Index (HI) - The harvest index was calculated using Formula VII. Formula VII: Harvest Index = Average seed yield per plant/ Average dry weight LO Statistical analyses - To identify genes conferring significantly improved tolerance to abiotic stresses, the results obtained from the transgenic plants were compared to those obtained from control plants. To identify outperforming genes and constructs, results from the independent transformation events tested were analyzed separately. Data was analyzed using Student's t-test and results were considered significant if the p value was less than 0.1. The JMP L5 statistics software package is used (Version 5.2.1, SAS Institute Inc., Cary, NC, USA). Experimental Results Tables 30-45 depict analyses of plant parameters as describe above overexpressing the polynucleotides of the invention under the regulation of the At6669 promoter under salinity irrigation conditions [NaCl 40-80 mM; NaCl Electrical conductivity (E.C.) of 7-10]. !0 Table 30 MAB 115 - Salt irrigation (40-80 mM NaCl) Event No. Control 8564.1 8565.1 A P A P Rosette Diameter on day 3* 1.70 1.80 0.09 1.75 0.04 Rosette Diameter on day 5 2.36 Rosette Diameter on day 8 3.77 4.00 0.09 Rosette Area on day 3 0.90 Rosette Area on day 5 1.56 1.70 0.09 Rosette Area on day 8 4.06 4.38 0.06 Plot Coverage on day 5 12.30 13.61 0.06 Plot Coverage on day 8 31.90 35.07 0.02 Leaf Number on day 3 5.08 5.94 0.00 Leaf Number on day 5 6.86 7.38 0.05 RGR of Leaf Number between day 3 and 5 0.18 RGR of Leaf Number between day 5 and 8 0.09 RGR of Rosette Area between day 5 and 8 0.53 0.62 0.01 Biomass DW [gr] 3.24 Harvest Index 0.11 0.15 0.07 0.16 0.03 Table 30: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. 25 122 Table 31 MAB54 - Salt irrigation (40-80 mM NaC) Event No. Control 8181.2 8182.2 8183.2 8185.4 A P A P A P A P Rosette Diameter on day 3* 1.70 1.95 0.04 Rosette Diameter on day 5 2.36 2.70 0.02 2.64 0.00 Rosette Diameter on day 8 3.77 4.00 0.08 Rosette Area on day 3 0.90 1.19 0.04 Plot Coverage on day 3 7.04 9.52 0.04 7.49 0.08 Leaf Number on day 3 5.08 6.19 0.06 Leaf Number on day 8 8.66 9.31 0.00 9.38 0.00 RGR of Leaf Number between day 0.18 3 and 5 RGR of Rosette Area between day 0.45 0.52 0.01 1 and 3 1000 Seeds weight [gr] 0.02 0.02 0.00 0.02 0.00 Yield [gr]/Plant 0.04 0.06 0.05 Harvest Index 0.11 0.17 0.01 Table 31: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 32 MAB55 - Salt irrigation (40-80 mM NaCl) Event No. Control 6802.10 6805.3 6805.4 A P A P A P Rosette Diameter on day 5 2.36 2.81 0.03 Rosette Diameter on day 8 3.77 4.29 0.05 Rosette Area on day 3 0.90 1.35 0.01 Rosette Area on day 5 1.56 1.70 0.03 Rosette Area on day 8 4.06 5.91 0.05 Plot Coverage on day 3 7.04 10.76 0.01 Plot Coverage on day 5 12.30 13.60 0.02 Plot Coverage on day 8 31.90 47.28 0.06 Leaf Number on day 3 5.08 6.25 0.00 Leaf Number on day 5 6.86 7.94 0.03 Leaf Number on day 8 8.66 9.50 0.01 RGR of Rosette Area between day 1 and 3 0.45 0.51 0.05 Yield [gr]/Plant 0.04 0.06 0.03 Harvest Index 0.11 0.15 0.05 Table 32: Provided are the growth, biomass and yield parameters of transgenic or control plants as 10 measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

123 Table 33 MAB56 - Salt irrigation (40-80 mM NaCl) Event No. Control 6691.2 6691.3 6693.2 6695.6 A P A P A P A P Rosette Diameter on day 3 1.70 1.89 0.05 1.97 0.07 Rosette Diameter on day 5 2.36 2.55 0.09 2.58 0.01 Rosette Area on day 3 0.90 1.06 0.00 1.15 0.02 1.23 0.08 Rosette Area on day 5 1.56 1.94 0.02 1.94 0.03 Rosette Area on day 8 4.06 4.63 0.02 Plot Coverage on day 3 7.04 8.45 0.00 9.21 0.01 9.82 0.07 Plot Coverage on day 5 12.30 15.49 0.01 15.53 0.02 Plot Coverage on day 8 31.90 37.03 0.01 34.82 0.05 Leaf Number on day 3 5.08 5.50 0.00 5.81 0.00 6.00 0.02 Leaf Number on day 5 6.86 7.38 0.01 7.50 0.02 1000 Seeds weight [gr] 0.02 0.02 0.08 Harvest Index 0.11 0.18 0.01 Table 33: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 34 MAB57 - Salt irrigation (40-80 mM NaCl) Event No. Control 6912.1 6912.13 6914.5 A P A P A P Leaf Number on day 3 5.08 5.69 0.00 Leaf Number on day 5 6.86 8.13 0.06 Leaf Number on day 8 8.66 9.56 0.06 RGR of Leaf Number between day 5 and 8 0.09 0.14 0.01 RGR of Rosette Area between day 5 and 8 0.53 0.62 0.02 0.58 0.10 1000 Seeds weight [gr] 0.02 0.02 0.00 Yield [gr]/Plant 0.04 0.06 0.01 0.06 0.03 0.06 0.01 Harvest Index 0.11 0.19 0.06 0.23 0.00 Table 34: Provided are the growth, biomass and yield parameters of transgenic or control plants as LO measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

124 Table 35 MAB58 - Salt irrigation (40-80 mM NaCl) Event No. Control 6783.3 7522.10 7522.3 7523.6 A P A P A P A P Rosette Diameter on day 3 1.70 2.11 0.00 Rosette Diameter on day 5 2.36 2.57 0.00 Rosette Diameter on day 8 3.77 4.06 0.07 4.54 0.00 Rosette Area on day 3 0.90 1.36 0.00 Rosette Area on day 5 1.56 2.29 0.00 Rosette Area on day 8 4.06 5.98 0.00 Plot Coverage on day 3 7.04 10.90 0.00 Plot Coverage on day 5 12.30 18.32 0.00 Plot Coverage on day 8 31.90 47.88 0.00 Leaf Number on day 3 5.08 5.63 0.06 6.06 0.00 Leaf Number on day 8 8.66 9.25 0.04 9.81 0.04 RGR of Leaf Number between day 0.09 0.12 0.03 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.08 Yield [gr]/Plant 0.04 0.06 0.09 Table 35: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 36 MAB59 - Salt irrigation (40-80 mM NaCl) Event No. Control 6791.6 6794.4 6794.5 A P A P A P Rosette Diameter on day 3 1.70 2.38 0.05 Rosette Diameter on day 5 2.36 2.73 0.06 Rosette Area on day 3 0.90 1.27 0.06 1.65 0.03 Plot Coverage on day 3 7.04 10.15 0.06 13.19 0.03 Leaf Number on day 3 5.08 6.44 0.05 6.00 0.02 6.75 0.01 Leaf Number on day 5 6.86 8.38 0.00 7.69 0.05 8.00 0.07 Leaf Number on day 8 8.66 9.38 0.02 Yield [gr]/Plant 0.04 0.06 0.06 Harvest Index 0.11 0.16 0.04 Table 36: Provided are the growth, biomass and yield parameters of transgenic or control plants as 10 measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

125 Table 37 MAB69 - Salt irrigation (40-80 mM NaCl) Event No. Control 6651.11 6651.12 6651.2 8342.1 A P A P A P A P Rosette Diameter on day 3 1.70 1.92 0.01 Rosette Area on day 3 0.90 1.09 0.00 Rosette Area on day 8 4.06 5.05 0.04 Plot Coverage on day 3 7.04 8.74 0.00 Plot Coverage on day 8 31.90 40.41 0.04 Leaf Number on day 3 5.08 5.69 0.00 Leaf Number on day 8 8.66 8.94 0.08 RGR of Rosette Area between day 0.45 0.49 0.02 0.51 0.04 1 and 3 1000 Seeds weight [gr] 0.02 0.02 0.00 0.02 0.01 Yield [gr]/Plant 0.04 0.05 0.09 0.06 0.02 0.07 0.01 Harvest Index 0.11 0.17 0.09 0.22 0.01 1 1 Table 38: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 39 MAB70 - Salt irrigation (40-80 mM NaCl) Event No. Control 7971.3 7972.1 7972.3 A P A P A P Rosette Diameter on day 3 1.70 1.94 0.00 Rosette Diameter on day 5 2.36 2.62 0.04 Rosette Diameter on day 8 3.77 4.40 0.00 Rosette Area on day 3 0.90 1.20 0.07 1.12 0.01 Rosette Area on day 5 1.56 2.06 0.05 1.87 0.00 Rosette Area on day 8 4.06 5.86 0.06 Plot Coverage on day 3 7.04 9.61 0.06 9.00 0.01 Plot Coverage on day 5 12.30 16.46 0.04 14.93 0.00 Plot Coverage on day 8 31.90 46.87 0.06 Leaf Number on day 3 5.08 5.94 0.09 Leaf Number on day 8 8.66 9.31 0.00 9.75 0.09 RGR of Rosette Area between day 5 and 8 0.53 0.62 0.01 Yield [gr]/Plant 0.04 0.08 0.00 0.07 0.01 Harvest Index 0.11 0.25 0.00 Table 39: Provided are the growth, biomass and yield parameters of transgenic or control plants as 10 measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

126 Table 40 MAB71 - Salt irrigation (40-80 mM NaCl) Event No. Control 7331.4 7331.5 7333.5 7334.5 A P A P A P A P Rosette Diameter on day 5 2.36 3.03 0.00 2.52 0.02 Rosette Diameter on day 8 3.77 5.01 0.05 4.33 0.01 Plot Coverage on day 5 12.30 19.90 0.09 14.25 0.08 Leaf Number on day 3 5.08 6.44 0.05 5.47 0.06 Leaf Number on day 5 6.86 8.13 0.00 7.56 0.00 Leaf Number on day 8 8.66 10.38 0.05 RGR of Leaf Number between 0.09 0.12 0.01 day 5 and 8 RGR of Rosette Area between 0.53 0.61 0.03 0.61 0.04 day 5 and 8 Yield [gr]/Plant 0.04 0.05 0.10 Harvest Index 0.11 0.21 0.06 Table 40: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 41 MAB72 - Salt irrigation (40-80 mM NaCl) Event No. Control 8552.1 8553.2 8553.3 8555.3 A P A P A P A P Rosette Diameter on day 3 1.70 1.90 0.05 1.83 0.00 Rosette Diameter on day 5 2.36 2.67 0.00 2.55 0.01 Rosette Diameter on day 8 3.77 4.06 0.08 4.15 0.10 Rosette Area on day 3 0.90 1.20 0.00 1.02 0.00 1.08 0.02 Rosette Area on day 5 1.56 1.88 0.07 2.00 0.00 1.87 0.00 Rosette Area on day 8 4.06 4.68 0.00 5.05 0.00 4.82 0.00 Plot Coverage on day 3 7.04 9.64 0.00 8.13 0.00 8.67 0.02 Plot Coverage on day 5 12.30 15.05 0.05 16.04 0.00 14.98 0.00 Plot Coverage on day 8 31.90 37.48 0.00 40.39 0.00 38.57 0.00 Leaf Number on day 3 5.08 5.81 0.00 5.88 0.00 5.56 0.00 5.50 0.00 Leaf Number on day 8 8.66 9.38 0.02 RGR of Leaf Number between 0.12 0.17 0.01 day 1 and 3 Table 41: Provided are the growth, biomass and yield parameters of transgenic or control plants as 10 measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

127 Table 42 MAB74 - Salt irrigation (40-80 mM NaCl) Event No. Control 7982.1 7983.6 7983.9 A P A P A P Rosette Diameter on day 3 1.70 2.06 0.10 1.94 0.00 Rosette Diameter on day 5 2.36 2.62 0.06 Rosette Diameter on day 8 3.77 4.05 0.02 Rosette Area on day 3 0.90 1.14 0.02 Rosette Area on day 5 1.56 1.83 0.05 1.85 0.10 Rosette Area on day 8 4.06 4.46 0.03 Plot Coverage on day 3 7.04 9.13 0.02 Plot Coverage on day 5 12.30 14.62 0.03 14.79 0.08 Plot Coverage on day 8 31.90 35.66 0.01 Leaf Number on day 3 5.08 5.75 0.04 5.31 0.07 Leaf Number on day 5 6.86 7.25 0.04 RGR of Rosette Area between day 3 and 5 0.37 0.42 0.07 1000 Seeds weight [gr] 0.02 0.02 0.02 0.02 0.05 Yield [gr]/Plant 0.04 0.06 0.05 Harvest Index 0.11 0.20 0.06 Table 42: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 43 MAB76 - Salt irrigation (40-80 mM NaCl) Event No. Control 7633.1 7633.2 A P A P Leaf Number on day 3 5.08 5.44 0.02 Leaf Number on day 8 8.66 9.13 0.07 Table 43: Provided are the growth, biomass and yield parameters of transgenic or control plants as LO measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 44 MAB77 - Salt irrigation (40-80 mM NaCl) Event No. Control 7931.11 8212.2 A P A P Leaf Number on day 8 8.66 9.75 0.09 RGR of Rosette Area between day 1 and 3 0.45 0.52 0.10 Harvest Index 0.11 0.15 0.07 15 Table 44: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

128 Table 45 MAB79 - Salt irrigation (40-80 mM NaCl) Event No. Control 7323.10, 7961.1, 7962.2, 7962.2, A P A P A P A P Rosette Diameter on day 3 1.70 1.84 0.10 1.93 0.05 Rosette Diameter on day 5 2.36 2.53 0.01 Rosette Diameter on day 8 3.77 4.32 0.03 4.01 0.04 Rosette Area on day 3 0.90 1.08 0.09 Rosette Area on day 8 4.06 5.29 0.03 4.78 0.05 Plot Coverage on day 3 7.04 8.63 0.08 Plot Coverage on day 8 31.90 42.32 0.03 38.27 0.05 Leaf Number on day 3 5.08 5.63 0.00 5.75 0.04 Leaf Number on day 5 6.86 7.44 0.01 RGR of Leaf Number between 0.09 0.11 0.01 day 5 and 8 RGR of Rosette Area between 0.53 0.59 0.01 day 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.00 Harvest Index 0.11 0.19 0.00 Table 45: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under salinity irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Tables 46-58 depict analyses of plant parameters (as describe above) overexpressing the polynucleotides of the invention under the regulation of the 6669 promoter under Normal Growth conditions [Normal irrigation included NaCl Electrical conductivity (E.C.) of 1-2]. LO Table 46 MAB115 - Normal Growth Conditions Event No. Control 8564.1 8565.1 8565.2 A P A P A P Rosette Diameter on day 3 1.67 1.80 0.09 1.75 0.04 1.95 0.04 Rosette Diameter on day 8 3.60 4.00 0.09 Rosette Area on day 5 1.54 1.70 0.09 Rosette Area on day 8 3.98 4.38 0.06 Plot Coverage on day 5 12.30 13.61 0.06 Plot Coverage on day 8 31.82 35.07 0.02 Leaf Number on day 3 5.25 5.94 0.00 Leaf Number on day 5 6.52 7.38 0.05 Leaf Number on day 8 8.92 9.31 0.00 RGR of Leaf Number between day 3 and 5 0.12 0.12 0.04 RGR of Rosette Area between day 5 and 8 0.53 0.62 0.01 Table 46: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 15 indicated days refer to days from planting.

129 Table 47 MAB54 - Normal Growth Conditions Event No. Control 8181.2 8182.2 8184.3 8185.4 A P A P A P A P Rosette Diameter on day 5 2.24 2.70 0.02 2.64 0.00 2.81 0.03 Rosette Diameter on day 8 3.60 4.00 0.08 4.29 0.05 Rosette Area on day 3 0.89 1.19 0.04 1.35 0.01 Rosette Area on day 8 3.98 5.91 0.05 Plot Coverage on day 3 7.10 9.52 0.04 7.49 0.08 10.76 0.01 Plot Coverage on day 8 31.82 47.28 0.06 Leaf Number on day 3 5.25 6.19 0.06 6.25 0.00 Leaf Number on day 5 6.52 7.94 0.03 Leaf Number on day 8 8.92 9.38 0.00 9.50 0.01 RGR of Leaf Number between day 0.12 0.13 0.08 3 and 5 RGR of Rosette Area between day 0.46 0.52 0.01 0.51 0.05 1 and 3 1000 Seeds weight [gr] 0.02 0.02 0.00 0.02 0.00 Table 47: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 48 MAB55 - Normal Growth Conditions Event No. Control 6802.5 6805.4 A P A P Rosette Area on day 5 1.54 1.70 0.03 Rosette Area on day 8 3.98 4.63 0.02 Plot Coverage on day 5 12.30 13.60 0.02 Plot Coverage on day 8 31.82 37.03 0.01 Table 48: Provided are the growth, biomass and yield parameters of transgenic or control plants as LO measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

130 Table 49 MAB56 - Normal Growth Conditions Event No. Control 6691.2 6691.3 6693.2 6695.7 A P A P A P A P Rosette Diameter on day 3 1.67 1.89 0.05 1.97 0.07 Rosette Diameter on day 5 2.24 2.55 0.09 2.58 0.01 Rosette Area on day 3 0.89 1.06 0.00 1.15 0.02 1.23 0.08 Rosette Area on day 5 1.54 1.94 0.02 1.94 0.03 Plot Coverage on day 3 7.10 8.45 0.00 9.21 0.01 9.82 0.07 Plot Coverage on day 5 12.30 15.49 0.01 15.53 0.02 Plot Coverage on day 8 31.82 34.82 0.05 Leaf Number on day 3 5.25 5.50 0.00 5.81 0.00 6.00 0.02 Leaf Number on day 5 6.52 7.38 0.01 7.50 0.02 RGR of Leaf Number between 0.12 0.13 0.06 day 3 and 5 RGR of Leaf Number between 0.12 0.14 0.01 day 5 and 8 RGR of Rosette Area between 0.53 0.62 0.02 day 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.08 Table 49: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 50 MAB57 - Normal Growth Conditions Event No. Control 6912.1 6912.6 6912.9 A P A P A P Rosette Area on day 8 3.98 4.48 0.02 Plot Coverage on day 8 31.82 35.81 0.01 Leaf Number on day 3 5.25 5.69 0.00 Leaf Number on day 5 6.52 8.13 0.06 8.13 0.06 Leaf Number on day 8 8.92 9.56 0.06 RGR of Rosette Area between day 5 and 8 0.53 0.58 0.10 1000 Seeds weight [gr] 0.02 0.02 0.00 Table 50: Provided are the growth, biomass and yield parameters of transgenic or control plants as 10 measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

131 Table 51 MAB58 - Normal Growth Conditions Event No. Control 6783.2 7522.10 7522.3 7523.6 A P A P A P A P Rosette Diameter on day 3 1.67 2.11 0.00 Rosette Diameter on day 5 2.24 2.57 0.00 Rosette Diameter on day 8 3.60 4.06 0.07 4.54 0.00 Rosette Area on day 3 0.89 1.36 0.00 Rosette Area on day 5 1.54 2.29 0.00 Rosette Area on day 8 3.98 5.98 0.00 Plot Coverage on day 3 7.10 10.90 0.00 Plot Coverage on day 5 12.30 18.32 0.00 Plot Coverage on day 8 31.82 47.88 0.00 Leaf Number on day 3 5.25 6.06 0.00 6.44 0.05 Leaf Number on day 5 6.52 8.38 0.00 Leaf Number on day 8 8.92 9.25 0.04 9.81 0.04 RGR of Leaf Number between day 0.12 0.12 0.03 5 and 8 1000 Seeds weight [gr] 0.02 0.02 0.08 Table 51: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 52 MAB59 - Normal Growth Conditions Event No. Control 6793.4 6794.4 6794.5 A P A P A P Rosette Diameter on day 3 1.67 2.38 0.05 Rosette Diameter on day 5 2.24 2.73 0.06 Rosette Area on day 3 0.89 1.27 0.06 1.65 0.03 Plot Coverage on day 3 7.10 10.15 0.06 13.19 0.03 Leaf Number on day 3 5.25 6.00 0.02 6.75 0.01 Leaf Number on day 5 6.52 7.69 0.05 8.00 0.07 Leaf Number on day 8 8.92 9.38 0.02 RGR of Rosette Area between day 1 and 3 0.46 0.49 0.02 1000 Seeds weight [gr] 0.02 0.02 0.00 Table 52: Provided are the growth, biomass and yield parameters of transgenic or control plants as 10 measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

132 Table 53 MAB69 - Normal Growth Conditions Event No. Control 6651.11 6651.12 8341.1 8342.1 A P A P A P A P Rosette Diameter on day 3 1.67 1.92 0.01 Rosette Area on day 3 0.89 1.09 0.00 Rosette Area on day 8 3.98 5.05 0.04 Plot Coverage on day 3 7.10 8.74 0.00 Plot Coverage on day 8 31.82 40.41 0.04 Leaf Number on day 3 5.25 5.69 0.00 5.94 0.09 Leaf Number on day 8 8.92 8.94 0.08 9.31 0.00 RGR of Rosette Area between day 0.46 0.51 0.04 1 and 3 1000 Seeds weight [gr] 0.02 0.02 0.01 Table 53: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 54 MAB70 - Normal Growth Conditions Event No. Control 7971.3 7972.1 7974.3 A P A P A P Rosette Diameter on day 3 1.67 1.94 0.00 2.27 0.00 Rosette Diameter on day 5 2.24 2.62 0.04 3.03 0.00 Rosette Diameter on day 8 3.60 4.40 0.00 5.01 0.05 Rosette Area on day 3 0.89 1.20 0.07 1.12 0.01 1.52 0.04 Rosette Area on day 5 1.54 2.06 0.05 1.87 0.00 2.49 0.10 Rosette Area on day 8 3.98 5.86 0.06 7.03 0.05 Plot Coverage on day 3 7.10 9.61 0.06 9.00 0.01 12.12 0.04 Plot Coverage on day 5 12.30 16.46 0.04 14.93 0.00 19.90 0.09 Plot Coverage on day 8 31.82 46.87 0.06 56.23 0.05 Leaf Number on day 3 5.25 6.44 0.05 Leaf Number on day 5 6.52 8.13 0.00 Leaf Number on day 8 8.92 9.75 0.09 10.38 0.05 RGR of Rosette Area between day 5 and 8 0.53 0.62 0.01 0.61 0.03 Table 54: Provided are the growth, biomass and yield parameters of transgenic or control plants as 10 measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

133 Table 55 MAB71 - Normal Growth Conditions Event No. Control 7331.4 7332.2 7333.5 7334.5 A P A P A P A P Rosette Diameter on day 5 2.24 2.52 0.02 Rosette Diameter on day 8 3.60 4.33 0.01 Rosette Area on day 5 1.54 1.88 0.07 Rosette Area on day 8 3.98 4.68 0.00 Plot Coverage on day 5 12.30 14.25 0.08 15.05 0.05 Plot Coverage on day 8 31.82 37.48 0.00 Leaf Number on day 3 5.25 5.47 0.06 5.81 0.00 Leaf Number on day 5 6.52 7.56 0.00 RGR of Leaf Number between 0.16 0.17 0.01 day 1 and 3 RGR of Leaf Number between 0.12 0.12 0.10 day 3 and 5 RGR of Leaf Number between 0.12 0.12 0.01 day 5 and 8 RGR of Rosette Area between 0.53 0.61 0.04 day 5 and 8 Table 55: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 54 MAB72 - Normal Growth Conditions Event No. Control 8552.4 8553.2 8553.3 A P A P A P Rosette Diameter on day 3 1.67 1.90 0.05 1.83 0.00 Rosette Diameter on day 5 2.24 2.67 0.00 2.55 0.01 Rosette Diameter on day 8 3.60 4.06 0.08 4.15 0.10 Rosette Area on day 3 0.89 1.20 0.00 1.02 0.00 1.08 0.02 Rosette Area on day 5 1.54 2.00 0.00 1.87 0.00 Rosette Area on day 8 3.98 5.05 0.00 4.82 0.00 Plot Coverage on day 3 7.10 9.64 0.00 8.13 0.00 8.67 0.02 Plot Coverage on day 5 12.30 16.04 0.00 14.98 0.00 Plot Coverage on day 8 31.82 40.39 0.00 38.57 0.00 Leaf Number on day 3 5.25 5.88 0.00 5.56 0.00 5.50 0.00 Leaf Number on day 8 8.92 9.38 0.02 Table 54: Provided are the growth, biomass and yield parameters of transgenic or control plants as 10 measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

134 Table 55 MAB74 - Normal Growth Conditions Event No. Control 7981.1 7982.4 7983.6 7983.9 A P A P A P A P Rosette Diameter on day 3 1.67 2.06 0.10 1.94 0.00 Rosette Diameter on day 5 2.24 2.62 0.06 Rosette Diameter on day 8 3.60 4.05 0.02 Rosette Area on day 3 0.89 1.14 0.02 Rosette Area on day 5 1.54 1.83 0.05 1.85 0.10 Rosette Area on day 8 3.98 4.46 0.03 Plot Coverage on day 3 7.10 9.13 0.02 Plot Coverage on day 5 12.30 14.62 0.03 14.79 0.08 Plot Coverage on day 8 31.82 35.66 0.01 Leaf Number on day 3 5.25 5.75 0.04 5.31 0.07 Leaf Number on day 5 6.52 6.26 0.02 7.25 0.04 Leaf Number on day 8 8.92 9.13 0.07 RGR of Leaf Number between 0.12 0.13 0.08 0.12 0.03 day 3 and 5 RGR of Rosette Area between 0.46 0.33 0.00 day 1 and 3 RGR of Rosette Area between 0.36 0.42 0.07 day 3 and 5 Biomass DW [gr] 3.07 1000 Seeds weight [gr] 0.02 0.02 0.02 0.02 0.05 Table 55: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. Table 56 MAB76 - Normal Growth Conditions Event No. Control 7633.1 7635.16 A P A P Rosette Diameter on day 3 1.67 1.93 0.04 Leaf Number on day 3 5.25 5.44 0.02 Leaf Number on day 5 6.52 7.25 0.04 Table 56: Provided are the growth, biomass and yield parameters of transgenic or control plants as 10 measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 57 MAB77 - Normal Growth Conditions Event No. Control 8212.1 8212.2 A P A P Leaf Number on day 3 5.25 5.63 0.00 Leaf Number on day 8 8.92 9.75 0.09 15 Table 57: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

135 Table 58 MAB79 - Normal Growth Conditions Event No. Control 7324.1 7961.1 7962.2 A P A P A P Rosette Diameter on day 3 1.67 1.84 0.10 1.93 0.05 Rosette Diameter on day 5 2.24 2.53 0.01 Rosette Diameter on day 8 3.60 4.32 0.03 4.01 0.04 Rosette Area on day 3 0.89 1.08 0.09 Rosette Area on day 8 3.98 5.29 0.03 4.78 0.05 Plot Coverage on day 3 7.10 8.63 0.08 Plot Coverage on day 8 31.82 42.32 0.03 38.27 0.05 Leaf Number on day 3 5.25 5.75 0.04 Leaf Number on day 5 6.52 7.44 0.01 RGR of Rosette Area between day 5 and 8 0.53 0.59 0.01 1000 Seeds weight [gr] 0.02 0.02 0.00 Table 58: Provided are the growth, biomass and yield parameters of transgenic or control plants as measured in Green House under normal irrigation. A = average; P = p value; RGR = Relative Growth Rate. The 5 indicated days refer to days from planting. EXAMPLE 7 IMPROVED FERTILIZER USE EFFICIENCY IN ARABIDOPSIS TISSUE CULTURE ASSAY LO Plants transgenic to the following MAB genes were assayed for fertilizer use efficiency in a tissue culture assay: MAB115, MAB54, MAB55, MAB56, MAB57, MAB58, MAB59, MAB69, MAB70, MAB71, MAB72, MAB74, MAB76, MAB77, MAB79, MAB116, and MAB117 (the sequence identifiers of the cloned polynucleotides and their expressed polypeptides are provided in Table 7 above). L5 Assay 1: plant growth at nitrogen deficiency under tissue culture conditions The present inventors have found the nitrogen use efficiency (NUE) assay to be relevant for the evaluation of the ABST candidate genes, since NUE deficiency encourages root elongation, increase of root coverage and allows detecting the potential of the plant to generate a better root system under drought conditions. In addition, there are indications in the literature 20 that biological mechanisms of NUE and drought tolerance are linked (Wesley et al., 2002 Journal of Experiment Botany Vol 53, No.366, pp. 13-25). Surface sterilized seeds were sown in basal media [50 % Murashige-Skoog medium (MS) supplemented with 0.8 % plant agar as solidifying agent] in the presence of Kanamycin (for selecting only transgenic plants). After sowing, plates were transferred for 2-3 days for 25 stratification at 4 'C and then grown at 25 'C under 12-hour light 12-hour dark daily cycles for 7 to 10 days. At this time point, seedlings randomly chosen were carefully transferred to plates with nitrogen-limiting conditions: 0.5 MS media in which the combined nitrogen concentration

(NH

4

NO

3 and KNO 3 ) is 0.75 mM (nitrogen deficient conditions) or 3 mM [Normall (optimal) 136 nitrogen concentration]. Each plate contains 5 seedlings of same event, and 3-4 different plates (replicates) for each event. For each polynucleotide of the invention at least four independent transformation events were analyzed from each construct. Plants expressing the polynucleotides of the invention were compared to the average measurement of the control plants (empty vector 5 or GUS reporter under the same promoter) used in the same experiment. Digital imaging and statistical analysis - Parameters were measured and analyzed as previously described in Example 5, Assay 1 above. Tables 59-68 depict analyses of seedling parameters (as describe above) overexpressing the polynucleotides of the invention under the regulation of At6669 promoter under Nitrogene LO Deficiency conditions. Table 59 MAB70 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. Control 7971.3 7972.1 7974.1 7974.3 A P A P A P A P Dry Weight [gr] 0.01 0.01 0.00 Fresh Wight [gr] 0.10 0.18 0.04 Leaf Area on day 10 0.36 0.47 0.04 0.55 0.00 Leaf Area on day 5 0.14 0.24 0.04 Roots Coverage on day 10 5.58 7.93 0.01 7.45 0.06 Roots Coverage on day 5 1.75 2.41 0.00 2.58 0.06 Roots Length on day 10 5.19 6.16 0.00 Roots Length on day 5 2.86 3.16 0.05 RGR of Roots Coverage between 0.45 0.67 0.05 day 5 and 10 RGR of Roots Coverage between 0.81 2.35 0.02 2.01 0.06 2.10 0.05 2.23 0.02 day 1 and 5 RGR of Roots Length between day 0.16 0.24 0.04 1 and 5 RGR of Roots Length between day 0.20 0.50 0.00 0.48 0.00 0.56 0.00 0.58 0.00 5 and 10 1 1 1 1 1 1 1 1 Table 59: Provided are the growth and biomass parameters of transgenic or control plants as measured in 15 Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

137 Table 60 MAB71 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. Control 7331.5 7332.2 7333.5 7334.4 A P A P A P A P Dry Weight [gr] 0.01 0.01 0.00 0.01 0.01 0.01 0.01 0.01 0.01 Fresh Wight [gr] 0.10 0.22 0.00 0.18 0.02 0.13 0.09 Leaf Area on day 10 0.36 0.55 0.00 0.52 0.00 Leaf Area on day 5 0.14 0.28 0.00 0.21 0.00 Roots Coverage on day 10 5.58 8.19 0.05 9.47 0.03 Roots Coverage on day 5 1.75 2.33 0.10 2.99 0.02 Roots Length on day 10 5.19 6.69 0.03 Roots Length on day 5 2.86 3.84 0.01 RGR of Roots Length between day 0.16 0.20 0.07 1 and 5 RGR of Roots Length between day 0.20 0.66 0.05 0.26 0.08 5 and 10 Table 60: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative 5 Growth Rate. The indicated days refer to days from planting. Table 61 MAB74 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. Control 7982.4 7983.9 A P A P Roots Coverage on day 10 5.58 9.76 0.06 Roots Length on day 10 5.19 6.70 0.00 RGR Leaf Area between day 5 and 10 0.30 0.44 0.09 RGR of Roots Coverage between day 5 and 10 0.45 0.63 0.08 Table 61: Provided are the growth and biomass parameters of transgenic or control plants as measured in LO Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 62 MAB76 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. Control 7635.4 A P RGR of Roots Coverage between day 5 and 10 0.45 0.70 0.07 RGR of Roots Length between day 1 and 5 0.16 0.26 0.07 15 Table 62: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 63 20 MAB77 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. Control 7931.11 8211.8 8212.2 A P A P A P Dry Weight [gr] 0.01 0.01 0.00 Fresh Wight [gr] 0.10 0.13 0.03 0.14 0.01 Roots Coverage on day 5 1.75 2.26 0.03 RGR of Roots Coverage between day 5 and 10 0.45 0.71 0.01 0.75 0.05 RGR of Roots Length between day 1 and 5 0.16 0.24 0.03 RGR of Roots Length between day 5 and 10 0.20 0.31 0.05 0.99 0.04 0.86 0.08 Table 63: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

138 Table 64 MAB79 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. Control 7323.3 7324.1 7961.1 A P A P A P Dry Weight [gr] 0.01 0.01 0.00 0.01 0.03 0.01 0.01 Fresh Wight [gr] 0.10 0.16 0.00 0.11 0.10 RGR of Roots Coverage between day 5 and 10 0.45 0.71 0.09 RGR of Roots Coverage between day 1 and 5 0.81 3.11 0.00 RGR of Roots Length between day 5 and 10 0.20 0.67 0.00 0.49 0.09 Table 64: Provided are the growth and biomass parameters of transgenic or control plants as measured in 5 Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 65 MAB115 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. Control 8561.2 8564.2 8565.1 A P A P A P Dry Weight [gr] 0.005 0.006 0.00 0.010 0.00 0.009 0.00 Leaf Area on day 10 0.24 0.27 0.00 Leaf Area on day 5 0.35 0.38 0.01 Roots Coverage on day 10 1.67 2.13 0.02 Roots Coverage on day 5 3.38 4.80 0.03 Roots Length on day 10 2.39 2.74 0.00 Roots Length on day 5 3.46 4.22 0.02 RGR Leaf Area between day 5 and 10 0.37 0.43 0.00 0.48 0.00 RGR Leaf Area between day 1 and 5 0.16 0.19 0.00 RGR of Roots Coverage between day 5 and 1.89 3.21 0.00 2.23 0.02 3.47 0.01 10 RGR of Roots Coverage between day 1 and 5 0.33 0.35 0.00 0.43 0.00 0.44 0.00 RGR of Roots Length between day 1 and 5 0.37 0.56 0.02 0.53 0.06 0.68 0.00 RGR of Roots Length between day 5 and 10 0.15 0.17 0.00 0.18 0.00 LO Table 65: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 66 15 MAB54 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. Control 8181.2 8182.2 8185.3 A P A P A P Dry Weight [gr] 0.005 0.009 0.00 0.008 0.00 0.007 0.00 Roots Coverage on day 10 1.67 1.80 0.04 Roots Coverage on day 5 3.38 4.27 0.02 3.40 0.00 Roots Length on day 10 2.39 2.52 0.01 Roots Length on day 5 3.46 4.11 0.02 3.50 0.00 RGR Leaf Area between day 5 and 10 0.37 0.45 0.00 RGR Leaf Area between day 1 and 5 0.16 0.17 0.04 0.19 0.00 RGR of Roots Coverage between day 5 and 1.89 3.59 0.00 3.60 0.01 2.20 0.01 10 RGR of Roots Coverage between day 1 and 5 0.33 0.52 0.00 0.55 0.00 0.36 0.00 RGR of Roots Length between day 1 and 5 0.37 0.70 0.00 0.73 0.00 0.51 0.02 RGR of Roots Length between day 5 and 10 0.15 0.21 0.01 0.22 0.01 0.17 0.00 Table 66: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting.

139 Table 67 MAB57 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. 6912.14 6912.20 6912.60 Control A P A P A P Roots Coverage on day 7 5.55 6.71 0.10 Roots Coverage on day 14 15.02 17.33 0.05 Roots Length on day 7 4.93 5.54 0.10 6.12 0.02 6.34 0.02 Roots Length on day 14 7.83 8.76 0.02 Fresh Weight 0.19 0.24 0.09 Table 67: Provided are the growth and biomass parameters of transgenic or control plants as measured in 5 Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. Table 68 MAB72 - Nitrogene Deficiency (0.75 mM Nitrogen) Event No. Control 8552.1 8552.4 8553.2 A P A P A P Dry Weight [gr] 0.005 0.01 0.00 0.01 0.00 0.01 0.00 Leaf Area on day 10 0.24 0.24 0.01 Roots Coverage on day 10 1.67 1.84 0.01 1.93 0.02 1.89 0.03 Roots Coverage on day 5 3.38 3.60 0.04 3.90 0.06 4.50 0.00 Roots Length on day 10 2.39 2.48 0.01 Roots Length on day 5 3.46 3.70 0.04 3.65 0.04 3.84 0.00 RGR Leaf Area between day 5 and 10 0.37 0.47 0.00 0.39 0.00 RGR Leaf Area between day 1 and 5 0.16 0.20 0.09 RGR of Roots Coverage between day 5 and 10 1.89 1.93 0.03 2.29 0.06 3.04 0.01 RGR of Roots Coverage between day 1 and 5 0.33 0.35 0.00 0.52 0.00 RGR of Roots Length between day 1 and 5 0.37 0.55 0.01 RGR of Roots Length between day 5 and 10 0.15 0.16 0.00 0.18 0.00 0.22 0.01 LO Table 68: Provided are the growth and biomass parameters of transgenic or control plants as measured in Tissue Calture growth under Nitrogene Deficiency (0.75 mM N). A = average; P = p value; RGR = Relative Growth Rate. The indicated days refer to days from planting. EXAMPLE 8 15 TRANSGENIC TOMATO AND ARABIDOPSIS PLANTS SHOW IMPROVED TOLERANCE TO SALT AND WATER-DEFICIENCY STRESSES UNDER FIELD CONDITIONS To test the impact of AQP TIP2 genes on plant's stress tolerance, the present inventors have previously cloned and overexpressed a polynucleotide which comprises the nucleic acid 20 sequence set forth by SEQ ID NO:2827 (also known as ABST36 set forth by SEQ ID NO:13 in W02004/104162; or SlTIP2;2) and which encodes the TIP2 polypeptide set forth by SEQ ID NO:2828 (which comprises the consensus sequence TLXFXFAGVGS; SEQ ID NO:2826). The nucleic acid constructs which comprises the ABST36 polynucleotide under the regulation of the constitutive Arabidopsis At6669 promoter (SEQ ID NO: 2823) (further referred to as the 25 At6669::ABST36 construct) was further transformed into tomato (Solanum lycopersicum) as a 140 model crop plant (Tom-ABST36), as well as into Arabidopsis thaliana. Four independent, T 2 transgenic tomato genotypes, overexpressing ABST36 in heterozygous form, were evaluated for their tolerance to salt and water deficiency in two different salt-stress field trials and one water deficiency-stress field trial consisting of two water-deficiency regimes. Transgenic genotypes in 5 each field trial were compared to their null-segregant counterparts as controls. Materials and Experimental Methods Tomato Salt-stress field trial - All field trials were performed in a light soil, in an open field (net-house) near Rehovot, Israel. The F1 hybrids of four independent events of the cross between ABST36-transgenic MicroTom plants and M82 tomato plants were grown for the first 3 LO weeks in a nursery under normal irrigation conditions. The seedlings were then transplanted into rows and grown in a commercial greenhouse. The salt-stress trial was divided into four blocks. In each block, two different irrigation systems were established: a normal water regime for tomato cultivation and a continuous irrigation with saline water (addition of 180 to 200 mM NaCl). Each block consisted of a total of 60 plants divided as follows: six plants per event and L5 six seedling null segregants were planted in the control row and a similar number of plants were planted in the salt-stressed row. At the stage of about 80 % red fruits in planta, fruit yield, plant fresh weight, and harvest index were calculated. Harvest index was calculated as yield/plant biomass. Tomato Water-deficiency-stress field trial - All field trials were performed in a light !0 soil, in an open field (net-house) near Rehovot, Israel. The F1 hybrids of the four independent events were initially grown as described above. Three-week-old seedlings were transplanted to a net-greenhouse. The experiment was structured in four blocks containing three rows irrigated with different water levels and intervals (WLI-0, WLI- 1, WLI-2). In each block, six transgenic plants per event analyzed and six non transgenic plants were transplanted in each row. 25 Seedlings were transplanted after 4 weeks into wet soil. The amount of water used to uniformly irrigate before transplanting reached maximum water capacity [20 % weight per weight (w/w)] at 60 cm depth, but without the creation of water overload. Each plant was transplanted near a dripper, with a 30-cm distance between plants, giving a total density of 2,600 plants per 1,000 2 m , according to a commercial growth protocol. Soil water capacity was measured using the 30 standard procedures by sampling soil from the following three depths: 0 to 20 cm, 20 to 40 cm, and 40 to 60 cm. The water content in these soil layers was measured routinely every week. The soil contained 5 % hygroscopic water while the maximum water capacity of the soil was 20 %. All fertilizers were applied in the soil prior to plant transplantation. The amount of both 141 phosphorus and potassium was calculated to be sufficient for all seasons. Nitrogen was applied as recommended, equally to all treatments, through the irrigation system. Each row contained 2 three dripping irrigation lines creating a coverage of nine drippers per 1 m . The water control was performed separately for each treatment. The soil was dried completely before the 5 beginning of the experiment. The different water regimes were begun only 4 weeks after transplanting when plants initiated the flowering stage. The amount of water supplied every week during the assay was calculated at the beginning of every week following the recommendations of standard growth protocols. WLI-0 treatment (control) received the recommended total weekly irrigation volume divided into three irrigations. WLI- 1 was irrigated LO three times a week, but the amount of water supplied was half that supplied to WLI-0. At the end of every week, WLI- 1 plants received the amount of water required to reach maximum soil water capacity. WLI-2 plants were irrigated only once a week, at the beginning of the week. The water-stress experiment lasted throughout the flowering period (23 days), corresponding to four cycles of the above-described stresses. Afterwards, all treatments received the L5 recommended amount of water. The calculated water amount was equal to the difference between the water contents in dry soil and in soil with maximum water capacity. At the end of each stress cycle, the water amounts were compared between treatments according to actual water content in the soil (S3). During the stress period, treatments WLI-1 and WLI-2 received a total of 75 % less water than the controls (WLI-0). !0 Experimental Results Transgenic plants exhibit increased tolerance to salt stress - To induce salt-stress, transgenic and control tomato plants were continuously irrigated in field trials with 180 to 200 mM NaCl. As shown in Figures 3a-c, 3g-j and Table 69 below, Tom-ABST36 plants appeared to be more vigorous in all of the experiments than the control plants, which were smaller and 25 showed severe symptoms of leaf and shoot necrosis (see for example, Figure 3j). This was also associated with higher fruit yield in Tom-ABST36 plants relative to controls (Figure 3a). Table 69 Salt-stress field trial Control 180 mM NaCl Plant FW Fruit yield Harvest Plant FW Fruit yield Harvest (tn/acre) (tn/acre) index (tn/acre) (tn/acre) index SlTIP2;2 ND 24.0 ND 2.8a 8.0 a 110% 2.8 WT ND 24.0 ND 1.4 3.8 0% 2.7 30 Table 69: Total yield (ton fruit/acre), plant fresh weight (FW), and harvest index were calculated for TOM ABST36 vs. control plants growing in the field under salt-stress conditions (180 mM NaCl). Results are the average of four independent events. a, b- Values in a column followed by different superscript letters are significantly different.

142 Transgenic plants exhibit increased tolerance to water-deficiency stress - Transgenic plants subjected to water-deficiency stress exhibited a significantly higher (26 %, p < 0.05) plant biomass compared to control plants (Figure 3e). Moreover the Tom-ABST36 plants showed a 5 significant (up to 21 %, p < 0.05) increment of fruit yield under water-deficient regimes (water level intervals WLI-1), while under normal irrigation, the yield improvement was even higher (27 %, p < 0.05; Figure 3d). The harvest index of the Tom-ABST36 plants was also higher when plants grew under regular and WLI- 1 conditions while it remained similar to control when the water-deficient regime consisted of once-a-week irrigation (WLI-2) (Figure 3f). LO The results from the three field trials provided strong evidence that the tomato Tom ABST36 plants show improved tolerance to salt and water-deficiency stress relative to the control plants, which is translated into significant increments in plant biomass and more importantly, fruit yield. Arabidopsis Salt-stress green house trial - A complementary experiment with L5 transgenic Arabidopsis plants expressing the ABST36 construct showed increased tolerance to a salt stress of 150 mM NaCl compared to control plants, as reflected in 42 % higher fresh biomass and 60 % higher dry biomass (Table 70 below). In-vitro salt-stress assay - Seeds of transgenic Arabidopsis plants harboring the At6669::ABST36 construct or 35S::GUS construct (which was used as control) were sown in !0 1/2 MS media containing 40 mg/l kanamycin for selection. Selected seedlings were sub cultured to 1/2 MS media with 0 or 150 mM NaCl. Plants were grown for a period of 3 weeks. Results are the average of four independent events that were analyzed in four repeats. For the determination of shoot dry weight, shoot plants were collected and dried for 24 hours at 60 'C and then weighed. 25 Table 70 Arabidopsis salt-stress assay 0 mM NaCl 150 mM NaCl Plant FW Plant DW Plant FW Plant DW Lines (mg) (mg) (mg) (mg) SlTIP2;2 408.28a 23.52a 68.55a 44a WT 394.36 22.63a 48.12 2.7 Table 70. Arabidopsis seedlings were grown in 0 and 150 mM NaCl under tissue-culture conditions. Shown are the fresh weight (FW) and total dry weight (DW) (both measured in milligrams) of ABST36 (SEQ ID NO: 30 2827) transgenic or wild type controls under normal conditions (0 mM NaCl) or salinity stress (150 mM NaCl). a, b Values in a column followed by different superscript letters are significantly different at P < 0.05 143 Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims. 5 All publications, patents and patent applications mentioned in this specification are herein incorporated in their entirety by reference into the specification, to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference. In addition, citation or identification of any reference in this application shall not be construed as an admission that such reference is 10 available as prior art to the present invention. To the extent that section headings are used, they should not be construed as necessarily limiting. Any reference to publications cited in this specification is not an admission that the disclosures constitute common general knowledge in Australia.

Claims (20)

1. A method of increasing abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80 % homologous to the amino acid sequence set forth in SEQ ID NO: 32 or 2766, thereby increasing the abiotic stress tolerance of the plant.

2. A method of increasing water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence at least 80 % homologous to the amino acid sequence set forth in SEQ ID NO: 32 or 2766, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant.

3. A method of increasing abiotic stress tolerance of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence exhibiting at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766, thereby increasing the abiotic stress tolerance of the plant.

4. A method of increasing water use efficiency (WUE), fertilizer use efficiency (FUE), biomass, vigor and/or yield of a plant, comprising expressing within the plant an exogenous polynucleotide encoding a polypeptide comprising an amino acid sequence exhibiting at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766, thereby increasing the water use efficiency (WUE), the fertilizer use efficiency (FUE), the biomass, the vigor and/or the yield of the plant.

5. An isolated polynucleotide comprising a nucleic acid sequence at least 80 % identical to the nucleic acid sequence set forth in SEQ ID NO: 6 or 2755.

6. An isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence exhibiting at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766. 145

7. A nucleic acid construct comprising the isolated polynucleotide of claim 5 or claim 6 and a promoter for directing transcription of said nucleic acid sequence in a host cell.

8. An isolated polypeptide comprising an amino acid sequence exhibiting at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766.

9. A plant cell comprising an exogenous polypeptide having an amino acid sequence exhibiting at least 80 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766.

10. The nucleic acid construct of claim 7, wherein said nucleic acid sequence is heterologous to said host cell.

11. A plant cell comprising the nucleic acid construct of claim 7 or claim 10.

12. A transgenic plant having increased abiotic stress tolerance as compared to a control plant, comprising the nucleic acid construct of claim 7 or claim 10.

13. The method of any one of claims 1 to 4, the isolated polynucleotide of claim 6, the nucleic acid construct of claim 7 or claim 10, the isolated polypeptide of claim 8, the plant cell of claim 9 or claim 11, or the transgenic plant of claim 12, wherein said amino acid sequence exhibits at least 85 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766.

14. The method of any one of claims 1 to 4, the isolated polynucleotide of claim 6, the nucleic acid construct of claim 7 or claim 10, the isolated polypeptide of claim 8, the plant cell of claim 9 or claim 11, or the transgenic plant of claim 12, wherein said amino acid sequence exhibits at least 90 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766.

15. The method of any one of claims 1 to 4, the isolated polynucleotide of claim 6, the nucleic acid construct of claim 7 or claim 10, the isolated polypeptide of claim 8, the plant cell of claim 9 or claim 11, or the transgenic plant of claim 12, wherein said amino acid sequence 146 exhibits at least 95 % sequence identity to the amino acid sequence set forth in SEQ ID NO: 32 or 2766.

16. The method of any one of claims 1 to 4, the isolated polynucleotide of claim 6, the nucleic acid construct of claim 7 or claim 10, the isolated polypeptide of claim 8, the plant cell of claim 9 or claim 11, or the transgenic plant of claim 12, wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 32, 2766, 31 and 2767.

17. The method of any one of claims 1 to 4, the isolated polynucleotide of claim 5 or claim 6, the nucleic acid construct of claim 7 or claim 10, the plant cell of claim 11, or the transgenic plant of claim 12, wherein said polynucleotide is selected from the group consisting of SEQ ID NOs: 6, 5, 2755 and 2756.

18. The method of any one of claims 1, 3 and 13 to 17, wherein the abiotic stress is selected from the group consisting of salinity, water deprivation, low temperature, high temperature, heavy metal toxicity, anaerobiosis, nutrient deficiency, nutrient excess, atmospheric pollution and UV irradiation.

19. The method of any one of claims 1, 3 and 13 to 17, further comprising growing the plant expressing said exogenous polynucleotide under the abiotic stress.

20. The nucleic acid construct of any one of claims 7, 10 and 13 to 17, or the plant cell of claim 11, wherein said promoter is a constitutive promoter, a tissue-specific promoter or an abiotic stress-inducible promoter. Dated: 25 August 2015