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AU2011266953A8 - Production of Biodiesel by yeast from lignocellulose and glycerol - Google Patents

Production of Biodiesel by yeast from lignocellulose and glycerol

Info

Publication number
AU2011266953A8
AU2011266953A8 AU2011266953A AU2011266953A AU2011266953A8 AU 2011266953 A8 AU2011266953 A8 AU 2011266953A8 AU 2011266953 A AU2011266953 A AU 2011266953A AU 2011266953 A AU2011266953 A AU 2011266953A AU 2011266953 A8 AU2011266953 A8 AU 2011266953A8
Authority
AU
Australia
Prior art keywords
esters
microorganism
fatty acids
lignocellulose
biodiesel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU2011266953A
Other versions
AU2011266953A1 (en
AU2011266953B2 (en
Inventor
Ambareesh Govind Phadnavis
Peter Ruhdal Jensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danmarks Tekniske Universitet
Original Assignee
Danmarks Tekniske Universitet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danmarks Tekniske Universitet filed Critical Danmarks Tekniske Universitet
Publication of AU2011266953A1 publication Critical patent/AU2011266953A1/en
Publication of AU2011266953A8 publication Critical patent/AU2011266953A8/en
Application granted granted Critical
Publication of AU2011266953B2 publication Critical patent/AU2011266953B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention is directed to a genetically modified microorganism for the extracellular production of free fatty acids and esters thereof, wherein said microorganism is characterised by a modified lipid biosynthesis metabolic pathway: for example reduced fatty acyl-coA synthetase activity that enables the microorganism to overproduce and secrete of esters of fatty acids (Biodiesel) into the surrounding medium, using one or more of: glucose, starch, lignocellulose and a glycerol-based substrate, as a carbon source. The invention further provides a method for the extracellular production of free fatty acids and esters thereof, comprising the use of said genetically modified organism, and a growth medium adapted for said method.
AU2011266953A 2010-06-18 2011-06-20 Production of Biodiesel by yeast from lignocellulose and glycerol Ceased AU2011266953B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US35634410P 2010-06-18 2010-06-18
US61/356,344 2010-06-18
EP10169839.7 2010-07-16
EP10169839 2010-07-16
PCT/EP2011/060237 WO2011157848A1 (en) 2010-06-18 2011-06-20 Production of biodiesel by yeast from lignocellulose and glycerol

Publications (3)

Publication Number Publication Date
AU2011266953A1 AU2011266953A1 (en) 2013-01-17
AU2011266953A8 true AU2011266953A8 (en) 2013-02-21
AU2011266953B2 AU2011266953B2 (en) 2014-09-18

Family

ID=43067173

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2011266953A Ceased AU2011266953B2 (en) 2010-06-18 2011-06-20 Production of Biodiesel by yeast from lignocellulose and glycerol

Country Status (6)

Country Link
EP (1) EP2582798A1 (en)
CN (1) CN103080305B (en)
AU (1) AU2011266953B2 (en)
BR (1) BR112012032279A2 (en)
CA (1) CA2802478A1 (en)
WO (1) WO2011157848A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2899431T3 (en) 2010-08-06 2022-03-11 Melt&Marble Ab Methods and products for the production of wax esters
CN103975070A (en) 2011-09-27 2014-08-06 艾克森美孚研究与工程公司 ACYL-ACP wax ester synthases
US8962299B2 (en) 2012-02-29 2015-02-24 Exxonmobil Research And Engineering Company Four-gene pathway for wax ester synthesis
EP2820029A4 (en) * 2012-02-29 2015-12-23 Exxonmobil Res & Eng Co FOUR GENES PATHWAY FOR THE WAIST ESTER SYNTHESIS
DE102012207921A1 (en) 2012-05-11 2013-11-14 Evonik Industries Ag Multi-stage synthesis process with synthesis gas
WO2015013674A2 (en) 2013-07-25 2015-01-29 The Regents Of The University Of California A yeast cell modified to overproduce fatty acid and fatty acid-derived compounds
SI3110930T1 (en) * 2014-02-28 2020-07-31 Delft Advanced Biofuels B.V. Process for the recovery of lipids or hydrocarbons
CN105543301B (en) * 2016-01-08 2019-07-05 武汉科技大学 The method of crude glycerine and ligno-cellulose hydrolysate cotransformation production microbial oil
CN107384923B (en) * 2017-08-01 2020-12-01 齐鲁工业大学 The promoter pYLG and its application in the construction of high-yielding long-chain dibasic acid in Candida tropicalis
CN118374367B (en) * 2024-06-19 2025-09-23 昆明理工大学 A kind of Zygosaccharomyces bisporus F3404 and its application in reducing trans fatty acids

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2000234587A1 (en) * 2000-03-31 2001-10-15 Idemitsu Petrochemical Co. Ltd. Process for producing lipids and lipid-secreting microorganisms
JP5933909B2 (en) * 2006-05-19 2016-06-15 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー Production of fatty acids and their derivatives
CN101490241A (en) * 2006-05-19 2009-07-22 Ls9公司 Preparation of fatty acids and derivatives thereof
WO2008080124A2 (en) * 2006-12-21 2008-07-03 Gevo, Inc. Butanol production by metabolically engineered yeast
WO2008113041A2 (en) * 2007-03-14 2008-09-18 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
US20100159546A1 (en) * 2007-03-30 2010-06-24 Aristos Aristidou Metabolic engineering of yeasts for the production of 1-butanol
WO2009143495A2 (en) * 2008-05-22 2009-11-26 President And Fellows Of Harvard College Fast-growing yeast

Also Published As

Publication number Publication date
AU2011266953A1 (en) 2013-01-17
CN103080305B (en) 2014-12-24
AU2011266953B2 (en) 2014-09-18
CN103080305A (en) 2013-05-01
BR112012032279A2 (en) 2018-08-28
WO2011157848A1 (en) 2011-12-22
EP2582798A1 (en) 2013-04-24
CA2802478A1 (en) 2011-12-22

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Legal Events

Date Code Title Description
TH Corrigenda

Free format text: IN VOL 27 , NO 2 , PAGE(S) 347 UNDER THE HEADING PCT APPLICATIONS THAT HAVE ENTERED THE NATIONAL PHASE - NAME INDEX UNDER THE NAME TECHNICAL UNVIERSITY OF DENMARK, APPLICATION NO. 2011266953, UNDER INID (71) CORRECT THE NAME TO TECHNICAL UNIVERSITY OF DENMARK

FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired