AU2009212783A1 - Soy depigmenting and skin care compositions - Google Patents

Description

- 1 AUSTRALIA PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT ORIGINAL Name of Applicant: Johnson & Johnson Consumer Companies, Inc. Actual Inventors: Miri Seiberg and Jue-Chen Liu and Stanley S Shapiro and John Kung and Rachel Grossman and Jonathan Miller Address for Service is: SHELSTON IP 60 Margaret Street Telephone No: (02) 9777 1111 SYDNEY NSW 2000 Facsimile No. (02) 9241 4666 CCN: 3710000352 Attorney Code: SW Invention Title: SOY DEPIGMENTING AND SKIN CARE COMPOSITIONS Details of Original Application No. 2006200823 dated 27 Feb 2006 The following statement is a full description of this invention, including the best method of performing it known to me/us: File: 32230AUP02 SOY DEPIGMENTING AND SKIN CARE COMPOSITIONS The present application is a divisional application of Australian Application No. 14433/01, which is incorporated in its entirety herein by reference. 5 Field of the Invention This invention relates to non denatured soy product-containing compositions that can be used for depigmentation, evening out skin tone and skin texture, skin firming, and care of the skin. More particularly, this invention relates to the use of soybean 10 containing compositions for depigmentation, producing skin of even tone and colour, increasing skin elasticity and reducing skin aging, increasing skin firming and promoting a younger look of the skin, for the prevention and treatment of sun-induced damage; for shine control, for the treatment and prevention of acne, treating cellulite and for inducing aesthetically pleasing skin feel. 15 Background of the Invention The understanding of the chemical and enzymatic basis of melanogenesis is heavily documented. Melanocytes migrate from the embryonal neural crest into the skin to produce secretory granules, melanosome, which produce melanin. Melanogenesis occurs within the melanosome, and the melanin is later distributed to keratinocytes via 20 the melanocyte dendrites. The key enzyme in melanogenesis is tyrosinase, which initiates a cascade of reactions that convert tyrosine to the biopolimer melanin. Two tyrosinase-related proteins (TRPs) are known, TRP-1 and TRP-2. These proteins share with tyrosinase about 40% homology and have catalytic activities as well as regulatory roles in melanogenesis. TRP-1 is the most abundant glycoprotein in melanocytes. 25 Skin colouring has been of concern to human beings for many years. In particular, the ability to remove hyperpigmentation, such as found in age spots, freckles or ageing skin, is generally of interest to individuals who desire a uniform complexion. In certain areas of the world, general body whitening is desirable. There have been many methods proposed to accomplish depigmentation. For example, kojic acid, hydroquinone, 30 retinoids and other chemical compounds have been used for depigmentation. Many of these previous solutions have not been found acceptable. There is often a distinct line of demarcation between the areas of skin to which such previous compositions have been applied. Therefore, precise application of allhese poundss is necessary in order t achieve he dsired result. Man of these compounds have also been found :o be quite irritating to the ski nand therefore uttdesirable for use. 5. Post-inflimmarory hyperpigmentation is a frequent pro lexm Fepresena virs .luteous disorders as wll'as therapeutic inmans Iowever the urderlyin ehanms and the vaiiability individuals show for developing st-iflanmmatory hper a pigmentation areiot well undersioodu.RlsedI-aser trearmentiae n effective for compound intradernial, or post-inflarninatory piginintati?4,and anot a ways

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0 M~ blfhrdahle H{ydz'oquinone and broad-ipectrun s reen are ouney e f's irapeuric treatment, but they 46 not completely prvr r elimI ate the } per pigmntaiy lesioris, A safe effctive and 'affordable treaiie p namraty her-pigmentationi is hII ird. Eyed £o-e desired is the nrpora treatment into a dailV skiti care pr to aehalht ad better obkihg Agingothe skin is a co lex pixiominehn rsukiug mh rtefe several intrinsic and ewtrie facos. Skin chaagesass iated with agir ofe i manifest as cosmetic disabilities, Due to its psychological impact agin of the skin' 201 has become an issue of great social significance and concern. Wirh baby boomers aging, the era of cosmetic :dae, cosn ierc maintenance and- tejvenadton gais . increased awgreness..Methods for preventing and tregitng skin a ing are highly dds~ire4.1 1aninsic aging is an inevitable gnt y programmed proceslxioug extriasic influences (windhear, cigarette smoke, chtemicals, :et.) ultraiolet S 25 radiation appears to be the single most important fatcir associated with aging o.he skin. Photoaging is induced by cumulative exposir to ultraviole radiation (UYR) increased recreational sun exposure, including excessive sunbathing, the depletion of stratospheric ozone, and the use of UVR in the treatment of various skn, diseases, have led to increased prevalence of photGoaging during the st'decade. Phordam ae 30 can be prevented by sun avoidance and proper sun protection, and cduld be reversed by the use of topical retinoids, which could be irritating and expensive 2 Overexposureto dihravioler and visible radiation also caUses sunburan.Th use of aspirin d other nonsteroidal ani-inflarnmatory drugs, cool baths aid topical stro a is offeronly mild relief his desired to have a single topical treatment that could prevn or revise skin aging'and provide yrotecrion or relief from snburn. It inod eired tb v a1s4ngIe and affoidable topital treameht for daily skin cIre, hichillprovide th Benefits, with no itriraton or neganine side effets 7 and which will further provide the desired homogeneousksin complexion, even out skit teure and tohe, and 1 nduce ski irming and a healthier younger look. c n- lt .w -e Acjdii isainfamm~tory dertuarologiicca disrde frequendy in' adolesceace andwith soe regulty in olde adu 'ofthe hna spe. Th too of ace can include skin lesions ranging fromihe coined in a Pi pioseceous foce o more severe copo-inflarmatory synpt ois such as pustnds papule cysts and nodules. The condition is noT only: unconfor tble for the victim, bu also embarrassing, and can result in disfiguremiennt ndcarr g. Many different approaches to arneliorAting this disorder have been stemipted 29 in the past, with nibme tearines more effective than others Atacks rnin or sirmpe washing and cleansing to pharmaceuticals have been employed. It is dditaed to have a ngle topical treatment that could prevent or reverse acne, which does nort require a pharmaceutical prescription, It is more'desired to 25 have a single and affordable topical treatnie o daily skin c-e, which will provide these benefits, with no irritadon or negative side effecCs and Which will further pvide the desired homogeneous skin complexion, even out skin texture and cone, ad induce skin firming and a heahhier look. 30 Dimpling of the skini of the thighs and buttocks is a phenomnnon commonly refrred to as dellulite, which affect women much more frequently than men. The -4 basic pathophysiology of cellulite has not been clearly identified. Theoretically, cellulite could reflect differences in adipose tissue biochemistry or connective tissue structure of affected versus unaffected individuals or regions within an individual. There is no evidence of any primary role for adipose tissue physiology, blood flow, or biochemistry 5 in the etiology of cellulite, although the connective tissue of the female thigh is structured to accentuate differences in small sub-dermal adipose tissue depots. Products aimed to cure cellulite (e.g. Cellasene) have been marketed all over the world, but their efficacy is in doubt. Methods to treat cellulite like the solid-probe ultrasound-assisted lipoplasty technique or syringe liposculpture result in some body contouring but are 10 painful and expensive, and needed repeated treatments. It is desired to have a topical affordable treatment that could prevent or reverse cellulite and induce skin firming, resulting in a healthier, younger look of the skin, and provide daily skin care with no irritation or negative side effects. In our previously filed PCT Patent Application WO 99/04752, the use of non 15 pasteurized soybean-derived extracts for the purpose of depigmenting skin was described. We have now found new stabilized and cosmetically superior compositions containing such soy products that contain less than 0.1% surfactants, have greater skin depigmenting activity and are less expensive to manufacture. We have unexpectedly found that soybean-containing compositions have anti-aging activity, they better 20 increase skin elasticity and firmness, and they are useful in the prevention and in the treatment of sun-induced damage and acne. Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common general knowledge in the field. 25 It is an object of the present invention to overcome or ameliorate at least one of the disadvantages of the prior art, or to provide a useful alternative. Summary of the Invention According to a first aspect, the invention provides a skin care composition for the topical delivery of a soy product comprising a non-denatured soy product and a 30 stabilizing system, said stabilizing system comprising a member selected from the group -5 consisting of an antioxidant, a chelating agent, or a preservative. Preferably the delivery of the soy product is to a mammal, such as a human. Preferably the non-non-denatured soy product is non-denatured soy milk or powder. Preferably, the compositions of this invention do not contain more than about 0.1% of a surfactant. In preferred 5 embodiments, the composition further comprises one or more other skin-related active agents such as, but not limited to, depigmenting agents, thickening agents, emollients, antioxidants or sunscreens. According to a second aspect, the invention provides a skin care composition for the topical delivery of a soy product consisting essentially of a non-denatured soy 10 product and a stabilizing system, said stabilizing system consisting of one or more of the members selected from the group consisting of an antioxidant, a chelating agent, and a preservative. According to a third aspect, the invention provides a skin care composition for the topical delivery of a soy product consisting essentially of a non-denatured soy product, a 15 thickening agent, and a stabilizing system, said stabilizing system consisting of one or more of the members selected from the group consisting of an antioxidant, a chelating agent, and a preservative. According to a fourth aspect, the invention provides a skin care composition for the topical delivery of a legume product comprising a non-denatured legume product and 20 a stabilizing system, said stabilizing system comprising a member selected from the group consisting of an antioxidant, a chelating agent, or a preservative. The invention also provides for use of the compositions of the invention for or a method of evening skin tone of a mammal; treating or preventing acne in the skin of a mammal; depigmenting the skin of a mammal; evening the texture of the skin of a mammal; 25 increasing the elasticity and firmness of the skin of a mammal; reducing the shine and oiliness of the skin of a mammal; treating the cellulite in the skin of a mammal; treating and preventing sunburn on the skin of a mammal. Unless the context clearly requires otherwise, throughout the description and the claims, the words "comprise", "comprising", and the like are to be construed in an 30 inclusive sense as opposed to an exclusive or exhaustive sense; that is to say, in the sense of "including, but not limited to".

- 5a The present invention also features a method of depigmenting the skin of a mammal, said method comprising the step of applying to the skin the above compositions. The present invention also describes a method to enhance the care of the skin. We 5 unexpectedly found that application of soymilk or soybean-derived compositions increase skin firmness and elasticity, even tone and texture, reduce skin aging, and are useful in the prevention and in the treatment of sun-induced damage and acne. This invention provides examples of soy-derived compositions useful for skin care. Other features and advantages of the present invention will be apparent from the 10 detailed description of the invention and from the claims. Brief Description of the Figures Figure 1 shows three graphs, demonstrating that soymilk can inhibit trypsin, inhibit PAR-2 cleavage, and inhibit keratinocyte phagocytosis. Figure 2 shows historical sections of swine skin treated with soy formulations and 15 stained to demonstrate pigment deposition. Figure 3 shows a picture of a flank of a dark skinned swine, treated with soymilk and DHLA formulations. Figure 4 shows histological sections of mice skins treated with soymilk and stained to document elastin fibers. 20 Figure 5 shows pictures of an individual excessively exposed to the sun, following treatment of a half of his face with soymilk.

F shows a magnificat on of the skin f an individual excess xzosd tot sn following treatrent f alof his face wit scymilk S figure 7 shows results of an acnegenicity and irr ancy stud n grphic fo M'iure a show i W en blot of soy formulations probed o j S n Pain I 8, -. - -, ..- 1.. W & Ihibitor). o Detailed Descripti of the Preferred Embodiment Iris believed that on i kiled in the art can based upon h descipdin herein, utilize d reni iventcion to its fullest exten The l lon sp nmb'ci to be Clonstrued a merely illustrated aad not iiidtttve f e 5 ander of the dislosuie in anyway whatsoever. Unless dened e se n and ientific terrs used herein have the same meing as cormnAly under ood by oiteof ordinary skill in the an to which dhe inyen on belo Also publications patent applicstios patents, and other referenes mentioed herein are ncorporated b#eii by reference. The navel doipoitios of this ivenion contain legume product, and preferably soy products, that may be in the form of a fluid (e0ym) or a solid (e.g., a soybean powder or soyzhilk powder). What is meant by "soy product" is a * substance de ied from the soybean, containing the ingredients narurally found in 2 soybeans at the relative concentrations as found in th beans. In one enibodimeht, the soy product is a non-denatured soy produit. "Denaturatin. is defined in the Bantam Medical Dictionary (1990 edition) as "the change in the physical and i physiology cal properties of a protein, that are brought about by hat; X orcheials These changes include fkss of activity (in the case of enzymes) and loss (or alteration) of 30 antigeniciry (in the case of antigens)". What is meant by "non-denatured soy product" is a soy product in which the processing for the derivation of such soy product (e, 6 the jtiperazure extraction ipedia) did got eliminate its protease hibitory amiry 6n ~ ~ t em)cbfIX -one eminbodiient the non-denatured state of the soy product of this jvention s measured by the presence of an intact soybean trypsin inhibitor (5Th prtein 5 In another embodiment, the soy product is soymilk. One way t make soyrnilk isto oak the soybean in deionited or purified water for several hor sah d them e ey were fully hydiated with the addition o raallquants of smal qunee itt ter(The griIag process allows thesoybean milk toe erected) cjlectin, the soybean 1ilk may be filtered to remove afty residual parts ofthe beAn 10,- husi The soyrailk used in this incrnio can be fresh soymilkas desiibed d o iab made from $oybean powdeandwaer. T sobAn wr i Soybeans .xd rna# also be Ijophilletd spray dr, or freezer ere a soyn ilk may or may ht be ie Such prepared soynilk y f abu 90 t dy e another exam s i f 15 dy k pdwdeu hde frbx lyophilized, spray dried or freezer h The a4dtioof ware' and finished with or without filtration or homogenzano Other metidds of soybean extraction could also be used to create the a tai ingredjens used i this invenion. Por e5ple, but not limid the aetie i gredients could be extracted from groundsoybeans using ethanol/water mixtues foliowd by the removal of the ethanol froi the extract, in such ways that the tease inhibitory activity of the soybean will be retained. *The compositions of the present invention maj contain froi about 1% to 25 bout 99%, by weight, bf the soy product. For example, when aiquid soy product (e g., oymilk) is sed, the composition may contain from about 500W to about 99% by weight; (e.g., from about 70% to about 99%) of the liquid soy product For examp when a solid soy product (e.g., soybean powder or soymilk powder) is used, the 'composition ray contain from about 1% to about 50%, by weight (e-g., froniabout 30 2% to about 30%,.by weight) of the solid soy product, Composition which comprises solid soy products may also comprise water (e.g., distilled water or water comained 7 within oymilk) to form-a liquid base to the composition (e.g., to forhi.a cretin, lotion, njectable solution or ge). Such composition may comprises from about 50% to Abo6t 98%, byweight (eg, froin about 70% to about 98 %, by weight) of waier. While not limited to these methods of administration, the compositions of this invention rmay be $ delivered topically, orally, or parenterally The soy products useful in this invention #y be produdid fro a soybean pieces, regardless of their gdographic origin, suh exposure 1 harvest ki nd the like However; specific strains, geographic origins or growth conditions ndghr be preferred 10' Foraniple, but not limiting to, oyean strains aotheeiu gains ritiirly hnheir t-yps ininhibitor (e1g SIf; I 3iBI) content or godito cha resulii trypsin nhibifor enrichinent in the bea_ might be pfrfefrd. Io ul~b * n'ted that the leg produ ts useful in the composi ions of this invtiahye' d irictive dr, which aybe tolerable in soine cues but iiiisd in od 1' niecessary the odor df the compositions of this invention may be reduced by using soybean products derived frain specific strains of soybeans known to produce reduced ador including but not limited to, lipoxypnase-2-deficient beans and those t ing modified sugar profile and the like. A press to reduee oxygen levels in the frmulation may also reduce the odor. Various masking agents or fagrances may 20 be used to rmask the odor. --In one denbodimenr, the compositions of this invention cotan aone or more preservatives. Due to the fact that the composition of thi invention are non . denatured (e compositions in which the protease inhibitory activity is reraid) 25 they may be more favorable as a medium for microbial growth. Preservatives are uief for substantially preventing microbial decomposition. Examples of preservatives inclode phenoxyerhanol and parabens such as methyl-paraben, ethyl-paraben, and propylparaben Other examples of preservatives are listed 6n pages 1654-55 fthe International Cosmetic Ingredient Dictionary and Handbbok, eds. Wenningci and '30 McEwen (CTA 7*" ed., 1997). hereinafter referred to as the "Cosmetic Handbook." The composition ay comprise from about 0.01% to about 20%, by weight (more aB referably' from about 0.5% to about 5%, by weight) of preservative. Micobial contamination caalso be eliminated by ammairradiatin or microkrarion or by brief bear treatmem that do not rest in the elimination of protease inhibitory 5 - ' Anrioxida ts and/or th acting agents may also be used to incr6ee h Ifc d stabiy of the CompoiStions. Artioxidants may be added both fQr fotmnu laon baibadlo nd fo cal effcaLy tioxidn cornpoind adrir d eri I." i ude bi are no te , w rolublc atiodants ch asulfi l 0 co pands and the rivatives (eug. sodium merabisulfite and Nacetylhystei4 poic aidad dihydrolipoiacid resveratra!, acavicysteine (ife e) latorn aid astorbic acid and acbrbicacid detivaives (Lg ascoby! palmit an as orby polyipeptid4 Oi 1661SOW It ddants suitib for use in the composions f tis invention iede butc, are not limited to, burylaed hydroxytolu&ne rqnoids (g. 15 retinpl and retinyl palmitate) toco ierols (e.g.) socopherol acetate),ocorrienols n ubiguinone. Natural extracts containing antioxidant suitable for use in the coinpositions 6f this iavention, include, but not limited to extracts containing noids and isfavdnids and their derivatives (e.g., gcniszeifi and diaizein) extracts cpntaiing resveratrol and the Jike-; Eiampls of such natural extracts include grped 20 seed, green tea, pirie bark,' propoliskiad legume extracts. Other xanples of iidan may be found on pages 1612-13 of the Cosmetic Haribbok- The conposmions of the present iennon may comprises the antioxdanrin amount o from about 0.001% t ab6ut 2O%, by weight (e.g,, from about 0.01% to about 10%.by weight) of the composition. 25 Cheating agents re also useful in asisting the stab iztion of the compositions of this invention. Examples of chelating agents include EDTA and derivatives thereof (e.g. disodiu EDTA and dipotassiunmEDTA), Idferie , lacto0crin, and citric aci 1 Other sampes of gheating agents are listed on page 1626 of the Cosmetic Handbook: 30 The compositions of the present invention may comprise the chelating agent in an 9 amount ofAfrom about 0.001% to abodt 20%, by weight (e.g from about 0.01% to ibout 10% by weight) of the composition. Thickening agents (e.g,, thickeners or viscosity enhancing ge udhlized in theonpositions pf this ihventa to alter their viscosiy he desired nj ...pro PC~dn(eg scsiy ops ill dpend upondte intended asu afl bath prod c - cream lriqn, or gel) For exanpidi appHcdn~esuc as barbd ahpoms h iscosity of the cnsiion should be relativel2low similar to an A pp a a cream, lotion, ot gel will have slightly hi her isos ( 10 abut 10c sid 100,000 ps) Thickening agent That cabe add[d t the coposiion o is inveto t ar siryilucd polymers chas polyacrylaes eg oyry d thr exnp f viscosir modifyins aree op pages 6929 ft Costic 15 Hardbopk To hieve the approprie viscosiry, compositions of theet inyendonmay compris froM about 0,01% to about 20%, by weigh ( from about 0.1% to about 5%, by weight) of a thickening agent. Thecompostions of this invention may also contain other dpigmenting agen MQ In addidon to the soy product. Examples of such depigmenting agents include but a noliani ed to, ipoic acid, dihvdrolipoic acid, rsveratroi co rbic a'id ko d hydroquinone; isoflavones, retinoids (g retiol retinoic acid, and retinA. panitate, ryrsinase inhibitors, melanosome transfer inhibitors, and selective cytroxic agents for rmelanocytes, or natural exracs containing these activities The amount of the 25 depigmenting agent used will depend gn the activity of the compound, and will typically range frotn about 0.001% to about 10%, by weght (e.g.

1 from about 0.01% to -about 10%, by weight) of the composition. The compositin of the present invention may also contam compounds that 30. enhance The feel of the composition on the skin of the user Examples of such compounds include, but are riot limited to, oils, silicones (eg, siloxane polymers such 10 -as diniehicone) and skin-conditioning agents such as emolients, and humecrants. gxznples of such skin conditioning agents may be found.of pages 1656-1670 of the Cosmetic Handbook. The composiions of this invention may also advant eously contain other cellulieornba aen inuding but not limited to, caffeinerehiol and their reinoids'and the like. Of course, other topical agents that may rve acvyn the skin, including anti-inflammatory agents such as c6rticdsteroids andi-pru ic agent TOPic analgesics, agens whch fight ae/ such as bezoyl eoxide a de y 10 uci as salicylic acid, anti-aging products include g retinoids rating alph hydidyv acids, co-enzyme-Q and others, antibigdcs axd antimycotics and shine contr61 products includin niconaze, keoconazole bio traconazoe and the like, and others known to those of ordinary skillin the be combed #i the cpn psitions of thi iunvention d used in the methods o this invention. Compositions of the present invention may be prepared by ming de d ngrediers For riple so Jk is mixed wih the cheating agent preserade ad/oi antioxidant. A thickeder is then added to the syst and Ie irreis further mixed uni iteaches honogeneiyc the desred viscosiy, The compoiqdS\ 0 of the present invertion may be prepared under an argon or nitrogen (or other inert gadous) blanket in order to enhance foimulation stability and/or to educ oybean odor. The compositions of thisimyention may be packaged in a tube, a sealed packet, a jar, pump, a botle, a can, a pledget, a toweler, a wipe or the lik n airtight package such as an aluninunx tube, aluminum pocket, puip, laminate rube, can also be used to 25 further enhance product stability. The cornposicins- of the present invention may be used to depigmaent the skin of a uammal (e.g., huian). What is meant by depigmentarion is the lightenig of the color of an area of skin, induding but not limited to, the global lightning of the user s 30 skin zone/comnplexion (e-g, the face, hands, or whold body, which is uneven as a resuul of aging skin, or darker than desired because of ethniciry or pathology, and the like), the evening of sl.nor tone, orthe specific Ughtenjng of age spots ree or darker PIented areas such as, but no limited to, post-iflamnaory hyper-pigmentary lesions. We unexpectedly found that the compositions of this invention ''lay be used to thumanskiniesulind reduced skin oiliess and hine The compo0i'ns of this nvenion icluding bu notlimired to soy-containing compos itions, wich contain protease inhibitory activity, when appled topically to the ski including i is a baft additive, or in a wip e educed the oAy and shin lok faci i ia on 10 prferd ermbolime.t t tin ei e admnisered i lea e d fo s th user wants the desied effet Coimosiridgs ofh present in veiin may also be ed to vnrtthe -condzrioi df abne. The compositiona$ of th s invention iay b pglie prfably 5 we per day for a period f rime preferably at least about 45 daysdeoinstrae an mproverient in te appearance of the skin and an arielirio'f the condliio However, it is thought that application of the composition for at ltabouttwo t fouiweek should provide an amelioration of prevention of the condition depend ng on the ndividuas sebaceous gland activity. 20 f another embod ment we found that soy products, suchs but not limiting to the compositions of tis invention, which contain prqrease inhibitory activity, when applied topically to the skin, including its use as i bath additive, or in a patch, assis in crearig a soothing cooling, and/or relaxing effect for the user, and an aesthedia 25.pleasing skin feeL The amount of composiion administered and the fregju:licy of administration will depend upon the couentration of the soy agent in thecomposition (eg, the soymrik), the condition of the skin aiea being treated, and the desired effect and, thus, will ultimately be determined by the user of the composition. In one embddimient, the composition is administered at least- once daily for as long as the user 30 wants; the desired effect, =Mo FovF we unexpectedly found that soy-milk treatment of hunan skin fo -wing t extessi&e exposure to the sun, relieved the pain and burning feelings and -duced the skin saliness associated with sunburn This invention provides a method for the soothing, cooling and pain reducing effects following sunbuin or excessive sun The -eh6 Aosk f'lil 0L-" 5 ethod of dal topical treatment at least ace per dy fr as long as the user wants the desired effect, of soyinilk containing compositions that obtain protease inhibitory activity In anotherehbodiment we unexpectedly found that pre-treatentof u an 'snihogrmilk (e g topical treatment use as bath additive) toulId protect from sun : bedsagnge. Skin ber-exposed to xhe sun following soynilk et tmnt as nots 6d, scaly, dry nd painful s he untrdeated slin of the same individual-Thi jvent provides a method for t prevention of sin scaling ednessand pai following excessive ;un exposure The method onsists of daily topical rregrment a least once 15 e day fpr alg athe uSer wants the desird effect of soynik contnng c osidons that contain protease inhibitory acaivity Thus, soymbilk and its derivatives can-provide skin care properties, icludin elasticity, prevent scaliness/tadness/pain of over exposure to the sun, after 20~O suh/sbuknt cooling sensation, bath; depignentation, even tone and texture of the skin post :inflamntory hyperpignentaion, acne and cellulite. As evidenced by the examples herein, we have unexpectedly found that the topical use of soynmilk results in an increase in the elastic properties of the treated ski. 25 Therefore sitable topical use for legume extras containng protease inhibitory activity, and in particular for soymilk and soybean formulation is to increase skin elasticity and firning 9 by affecting Elasrin and collagen fibers and skin plastic properies We unxiectedly fouhd that the topical application of soy products, icluding but not limiting to the compositions of this invention and of oUr previouis Invenon (PCT Patent Application WO 99/04752), which contain proeasAinhibitory activity, results in skin firing, increased skin elasticity, and anti-aging effects, and 13 induce an aestheically pleasing skia feel. This invention provides method for incresig s kinfirndag and skin elasticity, and reducing signs of skin agi hic u§ful fo r bodj parts, and preferably for the face, upper body, and of the skin of thighs and buttocks. The method consists of daily topical treatment at least once S daily for as long as the user wants the desired effect, of soymik contamig mpositifas that cona prptrea inhibitory anty The folf6wing examples illustrate §veraLorbodiqients of the COaposions of this invenion. Thehould not be utilized to limit or narrow the scope o mention A any way. nle ii Preparation of svmilk fron Soybean Powder 16 g of bean powder (Sunligh FoooJs, Taip Tai wa to abou 440 g of deoni zed watei. The nqacore was srir r qoi rempert 15 aout I hour. The midure was then filtered through a sieve having holes of 75! d amneter. The filtrate reslted in About I.1akg 6f soymilk. ExaMple 2; Pteparaion af'Soymilk Gel from Saymilk The following compositions of this invention were prepared as follows. The 20 weight percentages of each ingredient in The compositions are indicated below in. Tble and Tle e 2 First the soymilk, as prepared in example 1 wAi placed inta; st beaker. The preservaive Phenonip'| (a mixture of the preservatives methyL paraben, propyl-paraben, ethyl-paraben, and phenoxy-ethnol sold by NIPA, ilmington, Delaware) or the preservative phenoxyethanol were added to the - 25 soymilk. Nec, the chelating agent Disodiurn EDTA and in some examples the * umectant glycerin were added to the first beaker and mixed with the soymilk. It is ilW isuib1-tsfurter add tyclonethicoie;, or dimethicone-(radeaame-DoW- Corning 200 Fluid e), or PblySorbate 20, or Aluminum Starch Octyl Succinate, or Sucrose Cocoate, or PEG-6 Capric/Caprylic Triglycerides to the soynilk mixiure at 30 this step as required in some examples in Table 1 and Table 2. A mixture of the thickener polyacrylanide, laureth-7, and C13-14 isoparaffins (sold by Seppic, Paris 14 France under the Tradenae Sepigel*) was added to a second beaker along with the ni-oxidant BHT. The ingredients in the second beaker were then added to the ingredients of the first beaker and mixed until homogenous: The antid-xidants scorbic acid, sodium ascorby] phosphate, lactoferin, or tocopherol were then added S1 to the beaker and homogeneously raised io form the resulting 'el &E*0 le 3:5 Pr~aratks of ymilk Gel f om So bean dtive The fdllotvingomposonsmof this invention w&re pre aed as follows The weight prcitmage of each invm 4 iesieach athe prepatiois iid ted below in Table 2. first, %he sovrdik powder (Dev;ainsoy Farms, Carroll IA)orhe oybea der (Saligh Fodds, Taiwan)r the oyban Etied water were p acdd into aist leakr and, mied o reconsttut th p der h presevative Phendip an4dte cheliing agent Disodiub EDTA were cetn dd to 15 the first beaked mixed with he soymilk A mixrre of polyacrylarakle laureth 7, and C2l4soparaffxs was addd to a s acpnd beaker along with the anti-oxidant aT. The iagrediears in the second beaker Were then added to the ingredients 6f the first beaker and rixed untilhooao. 15 Table.1 vtt1 -15. Jr 20 Sb J 7.42% 89.04% 96.09% 96.05% .95,70% F~hen*,cthrro10.73% 77nx~n~adPr~n 1.00% 1.00%/ 1.00b% 1.00% Il y c p' in -- 2 6 % 2 0 AhmnuMn tsrch OcetA.Succlnat6.e 0.75%1 Sucrose Cocoete 1.00 1.00%. - PEG-anpo zp,4$iru r jpes, .. 3&Q% 3.00% _ _ DitoditiRr EDTA . 0.10 0.10% - .5 Po Oyaielt ue-/ 11 isopjarai 2.50% 2_751W 2.9090 o 2Z90% 3_20% PoIysorbate 20 . -0150% O01/ 00% 0053 100% 100%V 100%' 100%' 100 Table 2 24 .26 27 *.2f - 3. 34 35 Siykjij 04.40%. 92.40 9d30% 94.70% Phei6 Ptranbera s ati.n. 1O0% -voo% 1.00$ 100% 100% 1.n0 1.00% gi i) t. 5.00%.

'n5 00%% U5 Dis~dirin EDTA. 00e5% 0.0% . 05'h 0.O5% 005% 0d5% 0.05% P6alibsiiadLar7 4 [soprrafin6 3.50% 35*/ 3.20% A.20" 3.0% 320/ tdHdroxtoene 00S/5 0.05. 0.5% 005% 05/0 0 % A 05 O% %\6nfed Wi&: - 9070% 0s 9% 57"0b aoeP 1: % 1.00% CV eCiitge20O FKid 1.00/ $y9ilk P& ~der - 50 oyb6~dEitiz&t i sq ni enowdater Mbctura -. 1 TOTAL00% 100% 100% Example 4 ilk ihi bthe PAR 2 Pathway ur pa taplicationWO 95/04752 describes the- imporiabte * the PA pathwy in pignao a0 Shows that the soybean-derived protease inhibitor STI 10 inhibits t and nduces deigmentario Our patertappication WO 99/30729 fuither describes the role of PAIR in inducing keratinocyte phagocytosis and its effect on melahoso ei rza er and pigmentation. To test the possblity that soymilk induces depigtnentatio by inhibiang PAR-2 inducedphaocyosis the following experiment were performed First, ST aid soymrlk Were shown to act as S serine protease inhibit s, by inhibiting the trypsin-induced cleavage of a fltorescent casein peptide in a dose-dependent manner (Figure Ia). Total proease activity was measured using the EnzChekTmprotease assay kit, following manuLactuzer s instructions (Mvoleaulir Probes). STI and soymu were diluted ini PBS (Life Technologies) and incubated at 1 (v/v) with 1000 units of trypsin r pepared in PBS. 20 Following icubation with BODIPY fluorescent casein substrate at room temperature for one hour, fluorescece was measured (excitation 505/emission 515) ot-a SpecrraMax Gemini nicrorirer place reader (Molecular Dsvices Coiporadon) using SofLmax* Pro 3.0 software (Molecular Devices Corporation). Each experiment 171 was performed in six replicates and was repeated three times. The percent inhibition of trpsin cleavage of the substrate by S71ad soymilkwas calculated using Microsbft Excel and graphed in Sigma Plot. As shown in Figure la, soymilk can inhibit rypsin-induced cletvage in a dose response manner. designed tou tes thea insil 5 hIn a second experiment, a similar as ws designed to testh possible specific inhibition of soynilk on PAR- cleavage in this assay a synthei hrscet peptide comprising the cleavage sire of the hvi xan PAR2, S$GR I (a iilar assay is described in: Lourbakos A, Chinni C, Thoripson P; Ptemapa J 4! s, ackie Ej, Pike RN, Cleavage and activation of proteinase-activate eceptor- 2 on human nurraphih by gidgipain-R from Porphytoionas gin;vi PS Let 435: , 45-8, Sep 11, 1998), replaced the casein peptide as asnbsiate for tryp e peg a labeled with the fluotoph re pair Ed ncpg Montreal Canada , and was ed as a substrate fo A-2 sen pr 5 Itors a f this eptide with PAR 2 activators spchs yp co detected fluoresey (ex'itation 335 /rmision 515). The inhibition of inpsia induced PAR-2 pptide leavage by STI or So-ymilk was measured by incubating 100 ngirornolar peptide with10.units of trypsin (in 100mM TIS (hydroxymethyl]aminoinethane buffer, pH 80, bigene, Beltsville MD) with or S withour the test material, for one hour at room temperature, protected from light. Fluorescence was measured on Spectrifx* Germini microtker plate reader (MAleeglar Devices Corporati6n) using Softmax* Pro 3.0 software (MIolecular Devices Corporation). Each experiment was performed i six replicates and was repeated three times. The percent inhibition of trypsin cleavage ws calculated ng 25 Microsoft Excel and graphed in Sigma Piot. As shown in Figure lb, ihe deavage of this PAR-2 peptide by trypsin was completely inhibited in he presence of STL Soymilk retained the ability to inhibit this trypsin-induced PAR-2 cleavage (Fiure *2b), suggest that similar to STI, soybean extracts could inhibir PAR-2 activation. 30 To examine the possibility that soymilk could inhibit PAR-2 induced keratinocyre phagocytosis, we used the VybranfTm Phagocytdsis Assay (Molecular - 18 Probes). HaCaT keratinocytes were treated with SLIGRL, the PAR-2 activaring peptide, and increasing cncetratios of STI or soyilk for 24 hours, following by incubation with 100 microliter of fliresceit-labeled S. coli K-12 bioparricls for four hours. Itrracellular fluorecence, indicative of ingested E, coli particles, was S measured after ,rpari Ulue quenching of the non-internalized fluorescepce, Flnerescene was measured (485 nm exciration/538nm emission) ising a SpecttaMax Gemin nicrotier plate reader (Molecular Devices Corporation, Sunnyvale, CA). Data was collected ing Softrna* Pro 3.0 software (Molecular Devices Corporation), andas analyzed using SigTIaPlot 5.0 (SPSS Science 10 Chicago, IL) and SigmaStar 2.0 (SPSS Scince) software. 'Each experiment was petormed in six replicates and was repeated at least three times As shown in Figure e, SLIGK, increased keratinocyte-phagocytosis, and SI preveted thI nere s~ein a dose depedenr manner. Interestingly, freshly prepared soymilk 7ras to inii SLIGRL indtced phagocyrosis in keratinocytes to a similar degre. 5 *hese three experiment indicate that soynilk contains a prooase inhibitory activity that inhibits PAW-2 activation, which leads to reduced kerainocyte phagocytosis. This data suggest that the depigmenting activity 6f the soy products is due, in part, to theit PAR-2 inhibitory activity. ib -tI Famnle 5 The depiymnting acivity of new soymilk fornulaion is psnnrior Our patent application WO 99/04752 docUments the depigmenting active y. of soyniilk, and presents soyanilk-conraining formulations for te use of depigmemation, An example ofsuch formulation is Soy Essence I; as described in 5 Table 1 above. The present invention describes depigmenting compositions ecmprsi a soy produce and a stabilizng system but with n noie than 0,1% surdatant. An examplee of such foruiaion is Soy Essejice 235 as described in Table 1 abo To demnsrrate that Soy Essence 23is super to Esence 1 i its de pgenting actvi rhe filo&ring experinnt was performed.

I o Dark skinned Yucatan microswine (Charled iver, Maine) ere housedinappopriately sit6d cages in An envirdnnenhl y contlled room With'a 1 iur light- 16hi datka hoth tr supplied ith food and water ad ibitum Arimaj care& as ag 1s the "Guide for the Car and Use of Laboratory AnirnaldNon Publication No. 85-23 Twenty microliter of test compounds were applied topically, twice a'ay five dayshleek, fofeight rre weeks, on tedors'umto ahp day i das/eek o ih rnn the dof the swie, Treatments of individual swine were always arranged in a head total order on one side, and in a 20 tail to head order on the other side of the animal Biopsies were taken usingstandard techniques. All swine studies shoed no visual irritation, and histological analyses revealed no markers of iritation or other pathological signs. 25 Sections -oin biopsies Were stained with Fontana-Mason (FiM), using stadard procedures (Sheenan and Hrapckak, 1980), F&v staining identifies silver nitrate reducing molecules, In skin, this nn-specific stain identiferimarily melanin. At least hree sections per biopsy were processed, Each experiment was repeated at least three times -30 . Figure io~Ehr6-Jo l sectuis from dwo such exper;&ii-s.4he sectis -- were stained with fl4M, to mark the melanin deposition in the treaed. sites. The top panel shows the control (a) and the Soy Essence23 (b) treated sites of one swine. The lower panel shows the control (c) and the Soy Essence! (d) treated sites of 20 anothers*ine. It is obvious from ;h figure that the, both the relate and the absolute reduction in pigrnent deposition induced by Soy Essence 23 is superior to thie level induced by Soy Essencel. 5 xam ple i The deig mentin acrirvi ofsvmilk- i enhandid 'vhen combined with d ntioxidants Our parent applicadon WO 99/04752 documents the depib t active oynilk As we show here his dpigntingcivhy is due i t the inhibition of PAR-2 induced keratinocye phagocytosis To tst the hypothsis t improved depignentation could be achieved by combining mnre than e ~chismathat lead to depigmnentaion, we tested the depignenting c soymilk and anti-oidant combingons Swine were treated wihsqyrnik or Soymil. Essence foriulations 1% Is d hydrolipoic acid (DIILA), and M Reveratrol (Res) twice daily, Or with combination treatments of soymilk once daily and DHLA or R s once a day Vistsa akin lightening wa observed after seven or eight weeks of tear nt An exaniple of such experiments is demonstrated in Figure 3, showing a flank of a 4ark inned swne following 4ight weeks treatment with saymilk Esscuce auid DHLA 20 combination.. The left site(l) is treated with Soyrmilk Essenca23 a soyrn k formulation described above; The right site (3) is treated with DHILA, and the middle site (2) is treated with both Soymilk Essence 23 and DHLA This figure clearly demonstrates increased depigmentation following the combnation treatment fSoymilk essence 23 and DFUHA, relative to treatments with each ingredient alone. 25 Similar results were obtained using soyrnilk oroymilk essence, coribined with DHLA or Ret This example clearly demonstrates that additives of different mechanism can improve the depigmenting effect of soymnilk. Exampe 7: Sonilk affects skin elastic, 30 While perforning the soymilk studies described in our applications JBP-430, JBP-464 and JBP467, we unexpeczedly noticed that the skin of soymilk treated mice, u p palpatfelt more elastic. To test the possibility that soywilk has a positive ffec on skin elsticity, we performed two sets of experiments: non-invaslve elsticity measurements (ctaimeter measurements) and staining of skinection for Ehstit fibers. These stUdies indicate that soymilk treatment increases Ehstin fibers n skin, and the treated skin has increased elastic properties C3H and CMiB]6 mice were either induced for a new hItaiycl as described ,otapplicationts JB1430,JBP464 and J)P467, or shaved. Mict'vreeted wid oyndik daily as described in the.abive applications. At the scanfhexperiment (day 1 before fitst treaten) and at day 21, a cutaAete meaurement was perforne to measure skin elasticity. We used a cucometer avalale from Adaderr (MenloPark Califoria)pad exnployed the methods describedtin C;ouuraud ea, a"Skin Bimeebanidl1r Ppeies: In iva Evaluation of Influence of Age andBody Site by Nn-ivAsive Method," I Skin Res. and Techiol. 6-73 (1995)1,d Elier et a, M hanical Fropedties of iumanjForearin and Vulvar Skiin," 122 Br. J. Dermat.L P7-614 (1990) which aro'both incorporated herein by reference in their en rery Suction was applied through a 2 mm aperture and the corresponding skin displacement and recovery after release of the negadve pressure were measured. In huwtan studies, an improvement in- the nioS of defcrtatn parameters Ua/Uf (skin fatigue, or total 20 recovery fromthe load), Ur/Uf (biological elasticity, or elastic recovery ofrer loading and Ur/Ue (firr aess, or improkeienr in the deformation resistance of the siin) adicates better ronicity and elasticity of the skin. The defotmation parameters WU Ua, and Ur are dependent, in part, on skin thickness- Consequently, ratios were used for evaluation as described in Barel et al, "Suction Method for Measurement of Skin 5 MechanicalProperties The Cutorneter," Handbook of Non-Invasive Mlethods and the Skin 335-340 (1995) which is incorporated herein by reference in its entirety. An example of one such-experiment is shown in Tablk 3 demonstrating that soyznik treatment increases these paraiderers, which reflect iinproved skin elasticity. 30 While variations between animals were significant, the increase in ctnometric propenies was consistent, reflecting the beneficial effect of soyrnilk to skin elasticity 22 Table 3: Mechanical Properties of shaved soymilk-treated C3H m ice skin Bp hysica Day i Day 21 Parameter Uitreaied SoYiilk Untreated Soymilk Control Treated Cohrol Tread U 060 4d 0815 f/ 0139 44; 0,044 0.064 0,154 0.068 0.043 UrUe - '0943 4/A 0.964 +/- 0.6V - 1402+1 -)2 0. . 0&47 0.115 16 6 b 0067 To fiurthe tud this elasticity eff et s bicpsies werer n h c experintnc, an section* re staned fo Eltii h accordacc w h mnethods set, fdrch iiAKlignn, L., tuna'ss Teehuiiue, A Beaifui ti o s b(2) D opaud ote19900 1 h rtd herein by/ iefience in its entirety As shown in Figure 4. Elastin fiers (stained dark blue.purple) wee Ca d in thickness and eity around the skii appendages and the 'upper denmis o1he soymk- treated OCH mice (right panel) when compared to the untreated controls eftpanel)~ This increase was detectable as early as at the fonh day of treatment and 15 vVas consistent throughout the sidy. Sarne results *ee also obtained using the C57BI/6 mice. This example demonstrates that the use of soymilk and soymilk formiulations for skin care would increase skin elasticity and reduce Skin aging. hA separate expeIimefn, the effect of soy powder on collagen synthes s 20 studied in -viro. The effect of soy powder to enhance the rate of collagen ynthes in normal hunn dermal fibroblasts was assessed by evaluating the ixcorporarion of radioactive proline in extracelljlar collages after three days of treatment. As shown in Table 4, soy powder enhanced the rate of collagen synthesis in ormal human dermal-fibroblasts at I pg/znl by 58%. At lower concentrations, no signifcant 23 stimulaton was seen withsoy powder. Thus, the skin appeared more firm, full and more youthful Table.4; Effect 8f Soy powder on era-celulat collagen synthesis in normal human dermal fibroblasts. 5 coc ob 4. Collaptpyn 211568 1 6;3 1 5 7 0 .01: Scen -iif -iri S4 NS io sinal icam. Example 8: Se ilk cents s Iinduc -dmaa e. 10 One hunnaiindividual was treated with a og poitionccordng h avntion coraimag soynbilk on the righthalf of his faceonce dail shavin; After eight weeks of soyrnilk treatment, this ind duwas excessively exposed to the suna 4 ng recreational aiity, without de us of a screen The 15 individuals skin exhibited the symptoms o "sun-burn hs face tune ed and painful, and scaliness was developed by the second and third day after sun exposure. Vepectedly, the right side of his face, having been etreatad with a soymilk cbrhposition according to this invention, was not red painful b-scaly. igure 5 shows the two sides of the individual face The diferences in skin redness and in the degree 20 of surn damage are obvious from this figure. Figure 6 shows Hi-scope images of the individual's skint, demonstrating the scaliness of the untreated side aid the smoothness of the treated side. This exaoiple demtonstie that the routine use of soyrnilk compositions according to this inention, could protect the skin from unexpected strong sun-exposure, and reduce redness, scalihess and pain associated with "sun 25 burns' Example 9: Sovmilk cAn treat srat-induced d4ma. The individual described in Example 7 also used a soyrnilk composition on a part on his untreated side, only at 24 and 48 hours post sun-exposure. The applicaoibn 30 of soymnilk afr sun exposure served no; only to moisrarize the dramged skir; hwas 24 Soud io induce cooling and soothing sensation, to relieve the pain associated with the excesve sun expostire; and to reduce ridness, and scaliess while leaving A "fresh" eelin This ex ple demonstrates that topical application of soynilk or soymilk 6 niulatibns could relieve the pain and reduce the redness and scliness resulted from 5 ecessive sun exposure, The use oi soyrnilk on sun-exposed skin 1e*ds to a 6dol and fresh sensation. C Enmle 1C ntwainiConii tons Can Ridke Abkne Lesoins . 0 A clinical sandy was conduted lacider to study the effecof applying a- soy coXmuLping comgosiioktwice yet day for forty-five days upon subect hain Ald acne (ie 4 havg greater Ihan 10 non-inflamatormmator lesions at e art of the study) and uposubjeas haing to a A evat r a esio counts an assessment of eryrhena and a photograph assessment were made SO 15 Esnce23 containing 0.CQ% isofladie w7t applied to both groups o subjects In mild acne subjects, there was a highly significant decrease front baseine in the number of iaflaMMinatory papules (p6-0.001), a 419% decrease. Therewas also a dirtciral-t-rend toward a decrease in erythena (pe0.0 6 )) from baseline In ro acne 20 subjects, there was rosignificaut increase in comedones, papules or pustes This decreased nuniber of papules is illustrard graphically in Figure 7. F iM l l: gSonilk-containing Compositions of This fnvention CnaiZ Intact 2$ The scymillk-containing coipositions of this invention are no-denatured, and have protease-inhibitory activity. These compositions are uni ue because'they contain mtact porteains, and in particular intact ST, a serine ptorease inhibitor. To denionstrate this uniqueness, equal volumes of Essence 23, described above in* Ecnmple 3, and of a comrmercially available soy-based product (Helena Rubinstein's 0 FurureWhite'Essence, claiming "soja proteins" in their ingredients), were compared (or their STI content using Western blos, according to Bonlfacino J.S. (ad.) (1999) 25 Irmmunofluoresence Staining In Current Prdtocofl in Cell Biology on CD-ROM. John Wilej & Sons, Inc. (Teron Data Systemns, Jackson, WY). Samples were lysed in RIPA buffer[1% nonider-P40i 0.5% sodium deoxychclte, 01% sodiuni docedy sulfite, and Comuplete t protease inhibitors (Boehringer Mannheini, Indianapolis, hIN PBS, RIPA lysares (10 p.g per lane) were elecrophoresed in 10% SDS-PA GE gels and proteins wre analyzed by'ehhancedchenr niinescice (ECL) Wetmern S s Arnto Heights, I). Aniboies to st were from 0 heicon and were used at a 1:500 dilution. 2 As shown i.Figure 8, out soy formiulation Essne26otisnat ST 10 which is equl In Iz to an ST[ rer (hich is run in a parallel ne) In cdnrrast nether, the placebo of Essenc 23, nor the tbmmerciakoy-based product cnaans i-nat STI hi s understood the while the in entiohas been desc bed in cdnjuncticn ;th the detailed description thereof, that the foreg'-'ig desaiprion siteddt sC n1 -sr eres in Cdd - istr e and nor limit the scope of the which i define of the appended claims. Other aspects, advantages, and modifications are wi hin the c ams 26

Claims (27)

1. A skin care composition for the topical delivery of a soy product comprising a non-denatured soy product and a stabilizing system, said stabilizing system comprising a member selected from the group consisting of an antioxidant, a chelating agent, or a 5 preservative.

2. A composition according to claim 1, where said composition does not contain more than about 0.1% of a surfactant.

3. A composition according to claim 1 or claim 2, wherein said composition comprises an antioxidant. 10

4. A composition of any one of claims 1 to 3, wherein said composition comprises a chelating agent.

5. A composition according to any one of claims 1 to 4, wherein said composition comprises a preservative.

6. A composition according to any one of claims 3 to 5, wherein said antioxidant is a 15 member selected from the group consisting of BHT.

7. A composition according to any one of claims 4 to 6, wherein said chelating agent is selected from the group consisting of EDTA and derivatives thereof.

8. A composition of any one of claims 5 to 7, wherein said preservative is a paraben.

9. A composition of any one of the preceding claims, wherein said stabilizing system 20 comprises BHT, EDTA or a derivative thereof, and a paraben.

10. A composition of any one of the preceding claims, wherein said non-denatured soy product is soy milk.

11. A composition of any one of claims 1 to 10, wherein said non-denatures soy product is soy bean powder. - 28

12. A composition of any one of the preceding claims, wherein said composition further comprises a thickening agent.

13. A skin care composition for the topical delivery of a soy product consisting essentially of a non-denatured soy product and a stabilizing system, said stabilizing 5 system consisting of one or more of the members selected from the group consisting of an antioxidant, a chelating agent, and a preservative.

14. A skin care composition for the topical delivery of a soy product consisting essentially of a non-denatured soy product, a thickening agent, and a stabilizing system, said stabilizing system consisting of one or more of the members selected from the 10 group consisting of an antioxidant, a chelating agent, and a preservative.

15. A method of evening skin tone of the skin of a mammal, said method comprising the step of applying to the skin a composition of any one of claims 1 to 14.

16. A method of treating or preventing acne in the skin of a mammal, said method comprising the step of applying to the skin a composition of any one of claims 1 to 14. 15

17. A method of depigmenting the skin of a mammal, said method comprising the step of applying to the skin a composition of any one of claims 1 to 14.

18. A method of evening the texture of the skin of a mammal, said method comprising the step of applying to the skin a composition of any one of claims 1 to 14.

19. A method of increasing the elasticity and firmness of the skin of a mammal, said 20 method comprising the step of applying to the skin a composition of any one of claims 1 to 14.

20. A method of reducing the shine and oiliness of the skin of a mammal, said method comprising the step of applying to the skin a composition of any one of claims 1 to 14.

21. A method of treating cellulite in the skin of a mammal, said method comprising 25 the step of applying to the skin a composition of any one of claims 1 to 14. - 29

22. A method of treating and preventing sun burn on the skin of a mammal, said method comprising the step of applying to the skin a composition of any one of claims 1 to 14.

23. A composition according to any one of claims I to 14 further comprising retinol. 5

24. A composition according to any one of claims 1 to 14 further comprising retinol and caffeine.

25. A skin care composition for the topical delivery of a legume product comprising a non-denatured legume product and a stabilizing system, said stabilizing system comprising a member selected from the group consisting of an antioxidant, a chelating 10 agent, or a preservative.

26. Use of a composition according to any one of claims I to 14 for evening skin tone of the skin of a mammal; treating or preventing acne in the skin of a mammal; depigmenting the skin of a mammal; evening the texture of the skin of a mammal; increasing the elasticity and firmness of the skin of a mammal; reducing the shine and 15 oiliness of the skin of a mammal; treating the cellulite in the skin of a mammal; treating and preventing sunburn on the skin of a mammal.

27. A skin care composition for the topical delivery of a soy product; a method of evening skin tone of the skin of a mammal, treating or preventing acne in the skin of a mammal; depigmenting the skin of a mammal, evening the texture of the skin of a 20 mammal; increasing the elasticity and firmness of the skin of a mammal, reducing the shine and oiliness of the skin of a mammal, or treating cellulite in the skin of a mammal; use of a composition substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples.